CN1660408A - Medicine to be taken after being mixed in liquor of possessing bearutified and-faced effects - Google Patents
Medicine to be taken after being mixed in liquor of possessing bearutified and-faced effects Download PDFInfo
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- CN1660408A CN1660408A CN 200410104430 CN200410104430A CN1660408A CN 1660408 A CN1660408 A CN 1660408A CN 200410104430 CN200410104430 CN 200410104430 CN 200410104430 A CN200410104430 A CN 200410104430A CN 1660408 A CN1660408 A CN 1660408A
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Abstract
The present invention discloses a kind of liquor especially a liquor made from biological preparation and chinese medicine possessing beautified and faced effects.The compositiion of this injection includes Alpha-mannan peptides 10-40%, regulating metabolism chinese medicine 60-90%.That narrate components includes Alpha-mannan peptides10-40%,regulating metabolism chinese medicine 10-50%, antioxidant and scavenging free radicals chinese medicine 10-60%.That narrate components includes Alpha-mannan peptides 10-35%,regulating metabolism chinese medicine 10-40%,antioxidant and scavenging free radicals chinese medicine 10-50%,inhibition blach chromatogenesis chinese medicine 10-50%.This liquor makes the biological preparation and chinese medicine into a compound preparation and delays the aging of the skin by means of regulating body whole metabolism function,endocrine function,nourishing five viscera,conditioning blood-Qi essence and body fluid,dredging meridian etc.It plays the beautified and faced effects.
Description
Affiliated technical field
The invention belongs to a kind of electuary, particularly about the electuary with effects of beautification and nourishing face of a kind of biological preparation and Chinese medicine ingredients with effects of beautification and nourishing face.
Background technology
Aging is the irresistible natural law in the vital movement process of all organisms.The aging of human body skin, particularly often the aging of exposed skin of face is the easiest external presentation of seeing of examining of whole machine body.Motherland's traditional medicine is thought, at interior the five internal organs and body official key outside is an organic whole, the physiological function of the five internal organs and pathological change can reflect indirectly by the external various symptom and signs of human body, the shades of colour of human body skin, the change of state also can disclose the functional status of the five internal organs in the body, thus the degree of reflection whole machine body aging.Develop oral cosmetics according to this medicine principle, put forth effort to capture the inherent cause of disease that influences appearance, thereby make QI and blood full, skin is nourished, for looks improving and the skin nourishing is opened up a new approach.
Summary of the invention
People's skin and QI and blood are the mirrors of body interior, are all endocrine regulation as freckle, chloasma, acne and the pachylosis etc. of face, and the tyrosine Developmental and Metabolic Disorder is due to zang-fu disharmony and meridians are smooth.Under the normal condition, vital organs of the human body function is normal, equilibrium between yin and yang, and QI and blood is filled Sheng, and meridians are unobstructed, then make skin soft fine and smooth, ruddy gloss, hair is glossy black, and is radiant.The various skin disease can take place in deficiency of the liver and kindey, and its symptom is chronic process mostly, and xerosis cutis is plump coarse, desquamation, or alopecia, pigmentation, or association verruca, vascular nevus, pigmented spots, look unfamiliar dark etc., and often with hepatic and renal YIN deficiency syndrome.
The objective of the invention is to make a kind of electuary with effects of beautification and nourishing face, it makes a kind of compound granules with biological preparation and Chinese medicine are synthetic, by regulating the animal economy metabolic function, endocrine function, and nourish the aging that approach such as the five internal organs, the smart Tianjin of QI and blood regulating, dredging the meridian come delaying skin, play the effect of looks improving and the skin nourishing.
Technical scheme of the present invention is a kind of electuary with effects of beautification and nourishing face of design, it is characterized in that: its composition mainly comprises α-mannatide 10-40%, regulates metabolic Chinese medicine 60-90%.
Described composition includes α-mannatide 10-40%, regulates the Chinese medicine 10-60% of metabolic Chinese medicine 10 50%, oxidation and removing free radicals.
Described composition includes α-mannatide 10-35%, regulates the Chinese medicine 10-50% of metabolic Chinese medicine 10-40%, oxidation and removing free radicals, suppresses the Chinese medicine 10-50% that melanin forms.
Described composition includes α-mannatide 10-30%, regulates the Chinese medicine 10-40% of metabolic Chinese medicine 10-30%, oxidation and removing free radicals, suppresses Chinese medicine 10-50%, the Chinese medicine 10-60% of whitening function that melanin forms.
Described composition includes α-mannatide 10-20%, regulates metabolic Chinese medicine 10-30%, the Chinese medicine 10-30% of oxidation and removing free radicals, suppress that Chinese medicine 10-40%, the Chinese medicine 10-50% of whitening function, QI invigorating that melanin forms are enriched blood, the Chinese medicine 10-40% of nourishing the liver and kidney.
The metabolic Chinese medicine of described adjusting has natural pollen, Ganoderma, Folium Mori, Flos Ginseng, Flos Rosae Rugosae; The Chinese medicine of oxidation and removing free radicals has Radix Ginseng, Fructus Schisandrae Chinensis, Radix Angelicae Sinensis, Cordyceps, green tea, Semen Ginkgo, Radix Salviae Miltiorrhizae, Cortex Magnoliae Officinalis, Fructus Jujubae, Radix Pseudostellariae, Semen Ziziphi Spinosae, Radix Rehmanniae; The Chinese medicine that suppresses melanin formation has: ligustrazine, Fructus Corni, Radix Angelicae Sinensis, Aloe; The Chinese medicine of whitening function has: the Flos Sophorae Immaturus, Radix Notoginseng, Rhizoma Chuanxiong, pink, Radix Scutellariae, the Radix Angelicae Dahuricae, Margarita; The Chinese medicine of QI invigorating has: Radix Ginseng, Radix Codonopsis, the Radix Astragali, the Rhizoma Atractylodis Macrocephalae, Poria; The Chinese medicine of enriching blood has: Radix Angelicae Sinensis, Radix Rehmanniae Preparata, Radix Polygoni Multiflori, Fructus Lycii, Colla Corii Asini, Rhizoma Polygonati, Caulis Spatholobi; The Chinese medicine of nourishing the liver and kidney has: Cordyceps, Herba Dendrobii, the Radix Paeoniae Alba, Fructus Corni, Fructus Mori, Fructus Ligustri Lucidi, Herba Ecliptae, Radix Rehmanniae Preparata, Radix Achyranthis Bidentatae, Radix Polygoni Multiflori, Semen Cuscutae, Fructus Psoraleae, Poria.
Contain α-mannatide 400g, natural pollen 150g, Ganoderma 100g, Folium Mori 150g, Flos Ginseng 100g, Flos Rosae Rugosae 100g among the every 1000g of described electuary.
Contain α-mannatide 350g, natural pollen 50g, Ganoderma 50g, Folium Mori 120g, Flos Ginseng 80g, Flos Rosae Rugosae 100g, Radix Ginseng 30g, Fructus Schisandrae Chinensis 20g, Radix Angelicae Sinensis 20g, Cordyceps 20g, green tea 25g, Semen Ginkgo 30g, Radix Salviae Miltiorrhizae 10g, Cortex Magnoliae Officinalis 10g, Fructus Jujubae 35g, Radix Pseudostellariae 10g, Semen Ziziphi Spinosae 20g, Radix Rehmanniae 20g among the every 1000g of described electuary.
Contain α-mannatide 300g, natural pollen 50g, Ganoderma 50g, Folium Mori 50g, Flos Ginseng 50g, Flos Rosae Rugosae 100g, Radix Ginseng 30g, Fructus Schisandrae Chinensis 20g, Cordyceps 20g, green tea 25g, Semen Ginkgo 30g, Radix Salviae Miltiorrhizae 10g, Cortex Magnoliae Officinalis 10g, Fructus Jujubae 35g, Radix Pseudostellariae 10g, Semen Ziziphi Spinosae 20g, Radix Rehmanniae 20g, ligustrazine 30g, Fructus Corni 30g, Radix Angelicae Sinensis 50g, Aloe 60g among the every 1000g of described electuary.
Contain α-mannatide 200g among the every 1000g of described electuary, natural pollen 50g, Ganoderma 50g, Folium Mori 50g, Flos Ginseng 50g, Flos Rosae Rugosae 50g, Radix Ginseng 30g, Fructus Schisandrae Chinensis 20g, Cordyceps 20g, green tea 25g, Semen Ginkgo 30g, Radix Salviae Miltiorrhizae 10g, Cortex Magnoliae Officinalis 10g, Fructus Jujubae 35g, Radix Pseudostellariae 10g, Semen Ziziphi Spinosae 20g, Radix Rehmanniae 20g, ligustrazine 30g, Fructus Corni 30g, Radix Angelicae Sinensis 50g, Aloe 60g, Flos Sophorae Immaturus 20g, Radix Notoginseng 10g, Rhizoma Chuanxiong 10g, pink 20g, Radix Scutellariae 20g, Radix Angelicae Dahuricae 20g, Margarita 50g.
Contain α-mannatide 100g among the every 1000g of described electuary, natural pollen 50g, Ganoderma 30g, Folium Mori 20g, Flos Ginseng 30g, Flos Rosae Rugosae 50g, Fructus Schisandrae Chinensis 20g, Cordyceps 20g, green tea 25g, Semen Ginkgo 30g, Radix Salviae Miltiorrhizae 10g, Cortex Magnoliae Officinalis 10g, Fructus Jujubae 35g, Radix Pseudostellariae 10g, Semen Ziziphi Spinosae 20g, Radix Rehmanniae 20g, ligustrazine 20g, Radix Angelicae Sinensis 30g, Aloe 50g, Flos Sophorae Immaturus 20g, Radix Notoginseng 10g, Rhizoma Chuanxiong 10g, pink 20g, Radix Scutellariae 20g, Radix Angelicae Dahuricae 20g, Margarita 50g, Radix Ginseng 10g, Radix Codonopsis 10g, Radix Astragali 10g, Rhizoma Atractylodis Macrocephalae 10g, Poria 10g, Radix Angelicae Sinensis 10g, Radix Rehmanniae Preparata 10g, Radix Polygoni Multiflori 10g, Fructus Lycii 10g, Colla Corii Asini 10g, Rhizoma Polygonati 10g, Caulis Spatholobi 10g, Cordyceps 10g, Herba Dendrobii 10g, Radix Paeoniae Alba 10g, Fructus Corni 20g, Fructus Mori 10g, Fructus Ligustri Lucidi 10g, Herba Ecliptae 10g, Radix Rehmanniae Preparata 10g, Radix Achyranthis Bidentatae 10g, Radix Polygoni Multiflori 10g, Semen Cuscutae 10g, Fructus Psoraleae 10g, Poria 10g.
Characteristics of the present invention are: α-mannatide is a kind of biological response modifier, can regulate the various physiological functions of body and make it to be in poised state, and the resistance against diseases of energy enhancing body is in body and coordinates healthy state simultaneously; Natural pollen has enhancing and regulates metabolic effect, contain the nutritional labeling of a large amount of equilibrated needed by human body of comprehensive nutrition in the pollen, and all belong to natural activity, easily absorbed by human consumption, help regulating the metabolism of human body, have regulatory function in unique enhancing body.It can play dual regulation to hormonal system, not only enhance metabolism, the epiderm skin cell is upgraded to be accelerated, seem young, vigor is arranged, and inhibition promotes the activity of the tryrosinase that melanin forms, subtract light existing pigmentation, and positive preventive effect is arranged for the generation of new mottle.Natural pollen also has multivitamin effect, syngignoscism and defecating feces excretion etc.; Radix Ginseng has the inhibition lipid peroxidation, removes free radical, postpones effects such as cell ageing; Ligustrazine has and suppresses that melanocyte propagation, melanocyte are synthetic, the effect of tyrosinase activity; The Flos Sophorae Immaturus has the effect of blood-activating and qi-promoting, stasis-dispelling and pain-killing, is the whitening medicaments of using always; The sweet temperature QI invigorating of the Radix Astragali, benefit is defended consolidating superficial resistance, QI invigorating and blood producing; The Radix Polygoni Multiflori Preparata nourishing the liver and kidney, benefiting essence-blood complements each other with the Radix Astragali, can strengthen QI invigorating and enrich blood, the merit of nourishing kidney skin care; The Herba Schizonepetae dispelling wind for relieving itching, the treating blood disorders hemostasis; The Radix Glycyrrhizae invigorating the spleen and replenishing QI, coordinating the actions of various ingredients in a prescription; This four Chinese medicine concurrence QI invigorating is enriched blood, the effect of nourishing the liver and kidney, wind dispelling speckle removing.
The specific embodiment
Because the present invention is carried recoverable space craft (spacecraft or retrievable satellite) with the Alpha-hemolytic streptococcus strain, utilize the comprehensive function of factors such as little in the space (zero) gravity, cosmic ray, alternating magnetic field, fine vacuum, hyperpyrexia deep cooling, make the dna double chain structure fracture of bacterial strain, genome is reset, thereby produces new bacterial strain.After returning ground, bacterial strain through space treatment is cultivated and screened, by studying contents such as its morphological characteristic, cultural characteristic, biochemical reactions, metabolite, hereditary character and protein expression, hereditary stability, pilot scale production target, optimize the stable strain of plus variant, inherited character wherein, the medicine of the ulcerative colitis that after cultivation and fermentation, obtains medical treatment.This strain in China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation, is numbered CGMCCNo.1082.
Particularly Alpha-hemolytic streptococcus D33 bacterial strain behind ground screening, separation and the purification, selects higher, the secreted mannan peptide content of fermentation unit higher high yield, quality strains T33 strain through space treatment mutation.By Institute of Microorganism, Academia Sinica whole-cell protein SDS-polyacrylamide gel (SDS-PAGE) analysis of T33 bacterial strain and D33 bacterial strain and the genomic DNA restricted enzyme cutting analysis (RFLP/PFGE) of bacterial strain are compared, can prove that its gene of T33 bacterial strain that space mutagenesis and ground filter out has variation.This mutant strain is stable in heredity, stronger production adaptability is arranged, the mannan peptide content that is produced by its fermentation improves, content of peptides improves 3 to 5 times than national standard (80%), institute for drug control, Xi'an sample presentation is measured and is reached 271.6%, and fermentation content on average exceeds more than three times than matched group product content.This strain is through the spaceship-carried space flight of Shenzhou series of spacecraft, and further screening and culturing breeding forms stable hereditary property, the tangible high-yield highly-effective strain of variation features to strain by BeiJing ZhongKe Institute of Micro-biology of institute and biology department of Northwest University.The mannan peptide content of α in the every fermentation unit of this strain-space flight strain production has improved more than several times.(the material 1 of seeing Appendix: science and technology bureau Shaanxi Province, Shaanxi Province scientific and technological achievement assay certificate material
Adnexa material 2: the mannatide that the space flight strain is produced is produced bacterial strain and is carried notarization through " No. three, divine boat " airship.
Adnexa material 3: the mannatide that the space flight strain is produced is produced bacterial strain and is returned ground screening report through the lift-launch of " No. three, divine boat " airship
Adnexa material 4: Microbe Inst., Chinese Academy of Sciences produces the form Physiology and biochemistry probation report book of bacterial strain and ground contrast bacterium about the mannatide that " No. three, divine boat " airship carries the production of space flight strain
Adnexa material 5: Microbe Inst., Chinese Academy of Sciences produces SDS-PAGE and the RFLP/PFGE Analysis and Identification statement of bacterial strain and ground contrast bacterium about the mannatide that " No. three, divine boat " airship carries the production of space flight strain
Adnexa material 6: the mannatide oral liquid examining report that institute for drug control, Shaanxi Province " No. three, divine boat " space flight strain is produced
Adnexa material 7: scientific and technological novelty assessment report copy
Adnexa material 8: notice is accepted in China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation culture presevation)
Strain improvement of the present invention: on the basis of Alpha-hemolytic streptococcus growth rhythm, selected than the proper growth strain in period, adopt the plate growth bacterium colony, immerse methods such as the bacteria suspension in other material, germ-carrying sand, carry " No. three, divine boat " spacecraft on March 25th, 2002.Carried out 6 days 0 18 hours flying at space track (200 kilometers of perigee altitudes, 350 kilometers of altitude of the apogees) at rail.The specific condition of space mainly is presented as special environments such as microgravity, fine vacuum, high radiation, alternating magnetic field.The strain of outer-space flight be placed in one with the diverse environment of tellurian ecological environment in, the electronics, proton and the mental retardation heavy particle that mainly comprise coming self-magnetic field to capture; Cosmic ray such as proton, particle and heavier high energy heavy particle from the milky way galaxy; From the proton of sun magnetic storm and heavy particle etc.These particles act on the dna double chain structure in the microbial cell nuclear, can cause double-strand break.Microgravity, high vacuum environment can make the base sequence of DNA recombinate, promptly genomic reorganization and variation.The new bacterial strain that the variation back produces, variation in various degree all can take place in its morphological characteristic, cultural characteristic, biochemical reactions, metabolite, hereditary character and protein expression, pilot scale production target etc.After ground is returned in spacecraft, through further screening, only optimize wherein 0.2% plus variant and the stable bacterial strain of inherited character, make the production strain through cultivation.This strain that after spaceship-carried mutation, selects December in 2003 29 days by China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation, be numbered CGMCCNo.1082.
Experiment and middle trial production result show, stable on inherited character through the novel space strain behind the space mutagenesis, stronger production adaptability is arranged, the mannan peptide content that is produced by its fermentation improves, content of peptides improves 3 to 5 times than national standard (80%), institute for drug control, Xi'an sample presentation is measured and is reached 271.6%, and fermentation content on average exceeds more than three times than matched group product content, and fermentation period shortens greatly.
Preparation method:
The space flight strain is produced the preparation process of mannatide:
The mannatide of space flight strain of the present invention production is by following method preparation: the space strain preparation--purify, and final production goes out mannatide by one grade fermemtation--second order fermentation-----fermentation liquid.
1. space strain preparation:
With through the Alpha-hemolytic streptococcus of space mutagenesis breeding meticulous selection-breeding as producing strain.
A. inclined-plane seed culture medium: Carnis Bovis seu Bubali cream 0.5%, yeast extract 0.6%, peptone 0.5%, glucose 0.4%, sodium chloride 0.5%, agar 3%, sheep blood 8%; PH7.4.
121 ℃ of inclined-plane seed culture medium sterilising temps, 30 minutes time, steam pressure 0.12Mpa.
Unpacking strain cryovial with aseptic broth bouillon dilution, inserts the blood inclined-plane under aseptic condition, the rearmounted 38 ℃ of constant temperature culture of inoculation 30 hours.
B. meat soup seed culture medium: Carnis Bovis seu Bubali cream 0.5%, yeast extract 0.6%, peptone 0.5%, glucose 0.4%, sodium chloride 0.5%; PH7.4.
121 ℃ of meat soup seed culture medium sterilising temps, 30 minutes time, steam pressure 0.12Mpa.
With cultured slant strains under aseptic condition by 9% inoculum concentration, insert in the meat soup seed culture medium 38 ℃ of constant temperature culture 30 hours.
2. sweat:
Fermentation medium: Carnis Bovis seu Bubali cream 0.4%, yeast extract 0.5%, peptone 0.5%, glucose 0.4%, sodium chloride 0.5%.
121 ℃ of fermentation medium sterilising temps, 30 minutes time, steam pressure 0.12Mpa.
A. one grade fermemtation jar: with good meat soup strain under aseptic condition by 10% inoculum concentration, insert first class seed pot, 29 ℃ of constant temperature culture 30 hours, tank pressure was no more than 0.02Mpa, ventilation is advisable can stir culture fluid, fully stirs continuously.
B. second order fermentation jar: with the one grade fermemtation culture fluid under aseptic condition by 20% inoculum concentration, insert fermentation tank, 29 ℃ of constant temperature culture 70 hours, tank pressure is 0.02Mpa not, jar is put in deactivation.Deactivation is adopted to heat and is made the fermentation liquid temperature reach 100 ℃, is incubated 60 minutes, and cooling is left standstill.
3. leaching process:
A, fermentation liquid concentrate, and make concentrated solution volume and fermentating liquid volume ratio be controlled at 1: 15-1: 20 or be determined by circumstances.
The concentrated solution of b, fermentation liquid adds 80-99% ethanol, and the volume ratio is controlled at 1: 1.5-1: 5.5 or be determined by circumstances.Fully stir leave standstill after, centrifugal removal supernatant, the precipitation dissolved in distilled water, accent pH5.0 obtains lysate and lysate is left standstill.
C, the more centrifugal removal impurity of lysate is obtained supernatant, accurately measure the clear liquid volume, calculate required amount of alcohol by the supernatant stereometer, adjust pH slowly joins ethanol in the supernatant, and fully stirs, and leaves standstill the centrifugal removal supernatant in back and obtains precipitate.
D, precipitate reuse dissolved in distilled water are transferred pH, and centrifugal, supernatant adds ethanol precipitation again.Such technology repeatable operation to intermediate detection qualified till, the precipitate that obtains is the mannatide crude product that the space flight strain is produced.Vacuum drying 3-8 hour, promptly obtain the mannan peptide product that the space flight strain is produced.
The check of the product that said method obtains:
[character] this product is white or little yellow amorphous powder; Odorless, tasteless.
This product is easily molten in water, and is insoluble in ethanol, chloroform and acetone.
Specific optical rotation: get this product, accurate claim surely, be dissolved in water and be diluted to the solution that contains 10mg among every 1ml approximately, measure (two appendix vIE of Chinese Pharmacopoeia version in 2000) in accordance with the law, specific optical rotation is+70 ℃ to+80 ℃.
[discriminating] 1, get this product 10mg, add water 0.5ml and make dissolving, add a-naphthols alcoholic solution (5-100) 1ml, shake up, slowly add sulphuric acid 0.5ml along tube wall, after several minutes, the interface is aubergine.
2, get this product, add water and make the solution that contains 1mg among every 1ml, get about 10 μ l points on filter paper, dry, fix with dehydrated alcohol, put high salpeter solution (get periodic acid 1.2g, add water 30ml dissolving after.Add 0.2mol/L sodium acetate solution 1.5ml and ethanol 100ml, mixing promptly.Put the dark place and preserve, can use the several months) the middle immersion 5 minutes, take out, wash with 70% alcoholic solution, (get potassium iodide 5g, sodium thiosulfate 5g adds water 100ml and makes dissolving to put reducing solution, add ethanol 150ml, 2mol/L hydrochloric acid solution 2.5ml again, with adding, face the time spent and join with stirring) the middle immersion 5 minutes, take out, wash with 70% alcoholic solution, put in the pinkish red industry sulphuric acid test solution and soaked about 30 minutes, take out, (get sodium pyrosulfite 0.4g with sodium metabisulfite solution, add hydrochloric acid 1ml, being dissolved in water makes into 100ml, promptly), and flushing, dry, the place should be aubergine at the filter paper point sample.
3, get test sample and reference substance is an amount of, add respectively the chlorination sodium injection make contain 1mg among every 1ml solution as need testing solution and reference substance solution, press the test of mannatide immunogenicity determining method, test sample and contrast QC should all not have haemolysis to be taken place.
[inspection] 1, acidity: get this product, add water and make the solution that contains 10mg among every 1ml, measure (two appendix VIH of Chinese Pharmacopoeia version in 2000) in accordance with the law, pH value should be 3.0-5.0.
2, trap: get this product, add water and make the solution that contains 0.4mg among every 1ml, according to spectrophotography (two appendix VIA of Chinese Pharmacopoeia version in 2000), wavelength place at 260nm, its trap must not be greater than 0.25, and at the wavelength place of 280mm, its trap must not be greater than 0.20.
3, total nitrogen: get this product, measure according to N2 method (two appendix VIID second methods of Chinese Pharmacopoeia version in 2000).Press dry product and calculate, contain total nitrogen and should be 0.8-2.0%.
4, immunogenicity: get test sample and reference substance is an amount of, add the chlorination sodium injection respectively and make the solution that contains 10mg among every 1ml, make 1: 2,1: 4,1: 8,1: 16,1: 32,1: 64,1: 128,1: 256 diluent as need testing solution and reference substance solution with phosphate buffer respectively again, check in accordance with the law (attached mannatide immunogenicity determining method) that the least concentration of the insoluble blood vessel of test sample should be higher than more than a times of reference substance respective concentration.
5, loss on drying is got this product, is dried to constant weight at 105 ℃, subtracts weight loss and must not cross 5.0% (two appendix VIIIL of Chinese Pharmacopoeia version in 2000).
6, heavy metal is got this product, at 1.0g, checks that in accordance with the law (Chinese Pharmacopoeia version VIIIH in 2000) contains heavy metal and must not cross 20/1000000ths.
7, the undue toxicity gets this product, adds the chlorination sodium injection and makes the solution that contains 0.5mg among every 1ml, checks (Chinese Pharmacopoeia version appendix in 2000 XIC) in accordance with the law.Press the intravenous injection administration, should (injection) up to specification.
[assay]
1. the preparation of reference substance solution
Precision takes by weighing through 105 ℃ of D-mannose reference substance 0.1g that are dried to constant weight and puts in the 100ml measuring bottle, is dissolved in water and is diluted to scale.Shake up; Precision is measured 5ml, puts in the measuring bottle of 100ml, adds water to scale, shakes up.Contain mannose 50ug among every 1ml.
2. need testing solution is equipped with
It is an amount of to get this product, and accurate the title decides, and is dissolved in water and makes the solution that contains 40ug among every 1ml.
3. the preparation of standard curve
Precision takes by weighing reference substance solution 0,0.2,0.4,0.6,0.8,1.0ml, puts respectively in the tool plug test tube, respectively adds water to 1.0ml, adds 3% phenol solution 1.0ml again, shakes up, and pours sulphuric acid 4.5ml, shakes up, and is positioned over room temperature, is blank with 0 pipe.Measure trap according to spectrophotography (two appendix IVA of Chinese Pharmacopoeia version in 2000) at the wavelength place of 490nm.To corresponding trap, calculate regression equation with mannose ug number.
4. algoscopy
Precision is measured need testing solution 1.0ml, and from " adding 3% phenol solution 1.0ml again ", operation is in accordance with the law measured trap, by the content of regression equation calculation mannose under the sighting target directrix curve preparation.
Mannatide immunogenicity determining method (complement combined techniques);
1, test solution
A. phthalate buffer (pH7.2)
Get sodium chloride 8.5g, sodium hydrogen phosphate 0.565g and potassium dihydrogen phosphate 0.135g, add water to 1000ml and make its dissolving, add 10% Adlerika 1ml, shake up.
B.1% sheep erythrocyte suspension
The preparation of sheeps blood erythrocyte: (get glucose 20.5g, sodium citrate 8.0g, sodium chloride 4.2g in filling equivalent A Shi liquid by sheep jugular vein sterile blood sampling, citric acid 5.5g, add water to 1000ml and make dissolving, 100 ℃ the sterilization 30 minutes) sterile chamber in, 4 ℃ of preservations.
The preparation of 1% sheeps blood erythrocyte suspension: it is an amount of to get above-mentioned sheeps blood erythrocyte, and with sodium chloride injection washing three times, each centrifugal 5 minutes (2000 rev/mins), sheeps blood erythrocyte is amassed in pressure, makes 1% sheeps blood erythrocyte suspension with sodium chloride injection.Get suspension, make need testing solution for 20 times with the sodium chloride injection dilution, other gets 20 times of equivalent suspension thin ups as blank solution, according to spectrophotography (two appendix IVA of Chinese Pharmacopoeia version in 2000), wavelength place at 541nm measures, its trap should be 0.65-0.70, should regulate the concentration of 1% sheep erythrocyte suspension as overrun.
C. hemolysin and sensitization sheeps blood erythrocyte
The preparation of hemolysin: get above-mentioned hematocrit sheeps blood erythrocyte, make 25% sheeps blood erythrocyte suspension with sodium chloride injection.Get 1 of rabbit, the above-mentioned sheeps blood erythrocyte suspension of intravenous injection, once a day, and totally seven times, first three time 3ml, back three 5ml, last inject blood sampling in back seven days.Separation of serum, 56 ℃.Deactivation in 30 minutes, packing is preserved below 0 ℃.
The mensuration of amboceptor unit: it is an amount of to get hemolysin, add phosphate buffer and make 1: 1000,1: 2000,1: 3000,1: 4000,1: 5000,1: 6000,1: 7000,1: 8000,1: 9000,1: 10000 diluent respectively, respectively getting 0.1ml puts in the test tube, add 1% Sanguis caprae seu ovis cell suspension 0.1ml, shake up, add dilution factor and be 1: 30 guinea pig serum (complement) 0.2ml and phosphate buffer 0.2ml, shake up, 37 ℃ of insulations 30 minutes, the high dilution of complete hemolysis pipe is 1 unit hemolysin.
The preparation of sensitization sheeps blood erythrocyte: before facing usefulness, the sheeps blood erythrocyte suspension with 2% mixes with 2 unit haemolysis rope equal-volumes, and 37 ℃ are incubated 15 minutes promptly.
Complement: get the Cavia porcellus more than three, the heart blood sampling, centrifugalize serum is preserved below 0 ℃.
The mensuration of complement unit: it is an amount of to get complement, add phosphate buffer, make 1: 40,1: 60,1: 80,1: 100,1: 120,1: 140,1: 160,1: 180 diluent respectively, respectively get 0.2ml and put in the test tube, add the 0.2ml phosphate buffer, shake up, 37 ℃ of insulations are after 30 minutes, add sensitization sheeps blood erythrocyte 0.2ml respectively, shake up, 37 ℃ are incubated 30 minutes again.The high dilution of complete hemolysis pipe is the complement of 1 unit.
Antibody: it is an amount of to get the mannatide reference substance, add the chlorination sodium injection and make the reference substance solution immunizing rabbit that contains 10mg among every 1ml, the next day adopt ear vein injection reference substance solution once.Inject for the first time 0.2ml, for the second time respectively inject 0.5ml to the 5th time, respectively inject 1.0ml the 6th time to the tenth time, the tenth once respectively injects 2.0ml to the 15 time, and last is injected blood sampling in back three days, centrifugalize serum, 56 ℃ of deactivations in following 30 minutes, (before facing usefulness, needing 56 ℃ of deactivations once more in 30 minutes) preserved in packing below 0 ℃.
The mensuration of antibody unit: it is an amount of to get antibody, add phosphate buffer and make 1: 2,1: 4,1: 8,1: 16,1: 32,1: 64,1: 128 diluent respectively, respectively getting 0.1ml puts in the test tube, add mannatide reference substance solution 0.1ml and the 2 complement 0.2ml of unit, shake up, place more than 4 hours in 4-8 ℃, put 37 ℃ of insulations 30 minutes, the high dilution of insoluble blood vessel is 1 unit antibody.Establish antigen simultaneously and (do not add antibody, replace with the 0.1ml phosphate buffer), antibody (do not add antigen, replace with the 0.1ml phosphate buffer), complement (do not add antigen, antibody, replace with the 0.2ml phosphate buffer) control tube, more than three kinds of control tube haemolysis entirely; The sensitization sheeps blood erythrocyte that other establishes (do not add antigen, antibody and complement, replace with the 0.4ml phosphate buffer) control tube is haemolysis not.
2, immunogenicity determining method
It is an amount of to get rapid glycopeptide reference substance of manna and test sample, and the regulation under the photograph medicine item is made the reference substance solution and the need testing solution of variable concentrations, respectively gets 0.1ml and puts in the test tube, adds 2 antibody 0.1ml of unit and the 2 complement 0.2ml of unit.Shake up, more than 4 hours, put 37 ℃ of insulations 30 minutes in 4-8 ℃ of placement, add sensitization sheeps blood erythrocyte 0.2ml, shake up, 37 ℃ are incubated 30 minutes again.Observe the haemolysis situation of each pipe, the least concentration of insoluble blood vessel is represented the immunogenicity of mannatide.Establish antigen simultaneously and (do not add antibody, replace with the 0.1ml phosphate buffer), antibody (do not add antigen, replace with the 0.1ml phosphate buffer), complement (do not add antigen, antibody, replace) control tube with the 0.2ml phosphate buffer, more than three kinds of control tube haemolysis entirely: the sensitization sheeps blood erythrocyte that other establishes (do not add antigen, antibody and complement, replace with the 0.4ml phosphate buffer) control tube is haemolysis not.
Embodiment 1: get above-mentioned α-mannatide 100mg, natural pollen 50g, Radix Ginseng 120g, ligustrazine 120g, Flos Sophorae Immaturus 100g, Radix Astragali 150g, Radix Polygoni Multiflori 100g, Herba Schizonepetae 100g, Radix Glycyrrhizae 100g, totally 10 bags of amounts, every bag of 10g.Preparation process: Chinese medicine is dense fries in shallow oil twice with above back eight flavors, and decocting liquid filters, and it is standby that filtrate is concentrated into relative density 1.32-1.35 (80 ℃).Powdered natural pollen, α-mannatide and concentrated solution mixing, it is an amount of to add dextrin, and it is an amount of to add ethanol again, makes granule, oven dry, the qualified back packing of quality inspection finished product.Specifically produce one-tenth and equipment and saw " preparation technologies of 2000 editions electuaries of Pharmacopoeia of People's Republic of China " (Chinese Pharmacopoeia Commission compiles, and Chemical Industry Press publishes)
Other embodiment is identical with embodiment 1 production technology, just the component difference of medicine.
Contain α-mannatide 400g, natural pollen 150g, Ganoderma 100g, Folium Mori 150g, Flos Ginseng 100g, Flos Rosae Rugosae 100g among the every 1000g of the drug component of embodiment 2.
Contain α-mannatide 350g, natural pollen 50g, Ganoderma 50g, Folium Mori 120g, Flos Ginseng 80g, Flos Rosae Rugosae 100g, Radix Ginseng 30g, Fructus Schisandrae Chinensis 20g, Radix Angelicae Sinensis 20g, Cordyceps 20g, green tea 25g, Semen Ginkgo 30g, Radix Salviae Miltiorrhizae 10g, Cortex Magnoliae Officinalis 10g, Fructus Jujubae 35g, Radix Pseudostellariae 10g, Semen Ziziphi Spinosae 20g, Radix Rehmanniae 20g among the every 1000g of the drug component of embodiment 3.
Contain α-mannatide 300g, natural pollen 50g, Ganoderma 50g, Folium Mori 50g, Flos Ginseng 50g, Flos Rosae Rugosae 100g, Radix Ginseng 30g, Fructus Schisandrae Chinensis 20g, Cordyceps 20g, green tea 25g, Semen Ginkgo 30g, Radix Salviae Miltiorrhizae 10g, Cortex Magnoliae Officinalis 10g, Fructus Jujubae 35g, Radix Pseudostellariae 10g, Semen Ziziphi Spinosae 20g, Radix Rehmanniae 20g, ligustrazine 30g, Fructus Corni 30g, Radix Angelicae Sinensis 50g, Aloe 60g among the every 1000g of the drug component of embodiment 4.
Contain α-mannatide 200g among the every 1000g of the drug component of embodiment 5, natural pollen 50g, Ganoderma 50g, Folium Mori 50g, Flos Ginseng 50g, Flos Rosae Rugosae 50g, Radix Ginseng 30g, Fructus Schisandrae Chinensis 20g, Cordyceps 20g, green tea 25g, Semen Ginkgo 30g, Radix Salviae Miltiorrhizae 10g, Cortex Magnoliae Officinalis 10g, Fructus Jujubae 35g, Radix Pseudostellariae 10g, Semen Ziziphi Spinosae 20g, Radix Rehmanniae 20g, ligustrazine 30g, Fructus Corni 30g, Radix Angelicae Sinensis 50g, Aloe 60g, Flos Sophorae Immaturus 20g, Radix Notoginseng 10g, Rhizoma Chuanxiong 10g, pink 20g, Radix Scutellariae 20g, Radix Angelicae Dahuricae 20g, Margarita 50g.
Contain α-mannatide 100g among the every 1000g of the drug component of embodiment 6, natural pollen 50g, Ganoderma 30g, Folium Mori 20g, Flos Ginseng 30g, Flos Rosae Rugosae 50g, Fructus Schisandrae Chinensis 20g, Cordyceps 20g, green tea 25g, Semen Ginkgo 30g, Radix Salviae Miltiorrhizae 10g, Cortex Magnoliae Officinalis 10g, Fructus Jujubae 35g, Radix Pseudostellariae 10g, Semen Ziziphi Spinosae 20g, Radix Rehmanniae 20g, ligustrazine 30g, Radix Angelicae Sinensis 30g, Aloe 50g, Flos Sophorae Immaturus 20g, Radix Notoginseng 10g, Rhizoma Chuanxiong 10g, pink 20g, Radix Scutellariae 20g, Radix Angelicae Dahuricae 20g, Margarita 50g, Radix Ginseng 10g, Radix Codonopsis 10g, Radix Astragali 10g, Rhizoma Atractylodis Macrocephalae 10g, Poria 10g, Radix Angelicae Sinensis 10g, Radix Rehmanniae Preparata 10g, Radix Polygoni Multiflori 10g, Fructus Lycii 10g, Colla Corii Asini 10g, Rhizoma Polygonati 10g, Caulis Spatholobi 10g, Cordyceps 10g, Herba Dendrobii 10g, Radix Paeoniae Alba 10g, Fructus Corni 20g, Fructus Mori 10g, Fructus Ligustri Lucidi 10g, Herba Ecliptae 10g, Radix Rehmanniae Preparata 10g, Radix Achyranthis Bidentatae 10g, Radix Polygoni Multiflori 10g, Semen Cuscutae 10g, Fructus Psoraleae 10g, Poria 10g.
The toxicological study of looks improving and the skin nourishing electuary of the present invention: 1. acute toxicity test: rats by intraperitoneal injection 5ml: the 100g concentrated solution, not seeing has dead and any untoward reaction to take place.2. subacute toxicity test: oral 10g/100g/ day of rat, continuous 1 month; Oral 10g/100g/ day of rabbit, continuous 1 month, check liver, renal function, all no abnormal variation of hemogram and each organs and tissues.3. the Cavia porcellus hypersensitive test is negative.4. the deadly test of teratogenesis is all negative.
The animal pharmacodynamic study
[summary] purpose: study the melanocytic inhibitory action of five kinds of compound granules of the present invention to In vitro culture.Method: adopt mtt assay to measure the cell proliferation situation; It is synthetic that the NaOH cracking process is measured melanocyte; The Takahashi method is measured tryrosinase content.The result: five kinds of compound granules of the present invention have inhibitory action to melanocytic propagation, can make cell number obviously reduce (P＜0.05), and can make the synthetic significantly decline (P＜0.01) of melanocyte, make tyrosinase activity weaken (P＜0.01) gradually.Conclusion: five kinds of compound granules of the present invention can suppress melanocyte propagation, synthetic, the tyrosinase activity of melanocyte.Therefore, five kinds of compound granules of the present invention have the effect of looks improving and the skin nourishing.
1 materials and methods
1.1 material five kinds of compound granules of the present invention, α-mannatide provides by our company, vitamin C is the commercial goods, DMEMF12 dehydrated medium (U.S. GBCO company), 12-O-myristoyl phorbol-13-acetate (TPA, U.S. GBCO company), cholera mycin (CT, Sigma company), tetrazolium bromide (MTT, U.S. AMERSCO company), the basic fibroblast factor (bFGF, Hainan Hua Kang institute of biological products), levodopa (L-DOPA, U.S. Sigma company), the visible photometer of 753B1 microcomputer type ultraviolet (Shanghai), enzyme immunoassay instrument (ELASA analyser, the U.S.).
1.2 method
1.2.1 the specimen of peritomizing is got in the melanocytic cultivation of normal person, subcutaneous tissue is removed in the repeated multiple times flushing, is cut into little of 1mm * 2mm, 4 ℃ of refrigerator overnight digestion of DispaseII enzyme.Separate epidermis, muddy shape is blown and beaten in corium 0.25% trypsinization, and centrifugal back counting is with 5 * 10
6/ ml is inoculated in the culture bottle that contains the DMEMF12 culture medium, and its composition is 10% new-born calf serum, hydrocortisone 0.5 μ g/ml, insulin 0.5 μ g/ml, TPA10 μ g/ml, CT10 0.5 μ g/ml, bFGF 10 μ g/L, CT10 μ g/L.2-3 goes down to posterity standby after week.
1.2.2 DOPA dyeing is inoculated in passage cell on the coverslip, after adherent 48 hours, 10% formalin is fixed 2 hours, presses the dyeing of DOPA melanocyte reaction method, and visible melanocyte slurry is taupe brown or dark brown under light microscopic, turns out to be melanocyte.
The trophophase cell 1.2.3 the mensuration that melanocyte propagation suppresses is taken the logarithm, conventional digestion, regulating cell concentration is 5 * 10
5/ ml, every hole 100 μ l add 96 orifice plates, add five kinds of different compound granules (0.1g/L of the present invention after spending the night respectively, 0.1g/L, 0.1g/L, 0.1g/L, 0.1g/L) α-mannatide (0.1mg/L) and vitamin C (0.5mg/L) conditioned medium, cultivate after 48 hours, every hole adds the MTT (10 μ l) of 5mg/ml, continue to cultivate 3 hours abandoning supernatant, add dimethyl sulfoxide (DM-SO) 150 μ l, vibrated 10 minutes, adopt the enzyme immunoassay instrument at 490nm place survey light absorption value, the same period observation of cell under light microscopic metamorphosis and carry out cell counting.
1.2.4 the synthetic mensuration of melanocyte adopts the Hunt method to measure the melanocyte melanocyte synthetic influence of five kinds of compound granules of the present invention to In vitro culture.The take the logarithm cell of trophophase is adjusted to 2 * 10 with cell concentration
5/ ml, add 2ml in the culture bottle of 25ml, totally 8 bottles, random packet adds different conditioned mediums (the same) after 12 hours, continue to cultivate 72 hours, abandon culture fluid, 0.25% pancreatin Qinghua is with the centrifugal 10min of 1000r/min, abandon supernatant, added in l to 37 ℃ of water-bath of 1mol/L NaOH 100 μ 1 hour, distilled water 400 μ l dilution is at 475nm place survey light absorption value.
1.2.5 the mensuration of tyrosinase activity adopts the Takahashi method to measure the influence of five kinds of compound granules of the present invention to the melanocyte tyrosinase activity of In vitro culture.The cell inoculation method is the same.
1.2.6 this experiment statistics of statistical procedures data are all with mean ± standard deviation (X ± S) expression.Relatively adopt T check in groups between group.
2 results
2.1 the MTT measurement result sees Table 1.
The various medicines of table 1 are to the influence of the melanocyte propagation of In vitro culture
| Group 10 5/ml) | Drug level | Cell number (* |
| Blank group first kind of electuary group second electuary of the present invention group the third electuary group of the present invention the 4th kind of electuary group of the present invention the 5th kind of electuary group α of the present invention-mannatide group vitamin C group of the present invention | ????—— ????????0.1g/L ????0.1g/L ????0.1g/L ????0.1g/L ????0.1g/L ????0.1mg/L ????0.5g/L | ????4.2 ????2.32* ????2.07* ????1.7** ????1.68** ????1.50** ????3.53 ????3.01 |
Annotate: compare with the blank group
*P＜0.05,
*P＜0.01.
The experimental data of table 1 shows: five kinds of compound granules of the present invention all demonstrate the inhibitory action tendency to the melanocyte of In vitro culture propagation, and along with the compound granules prescription constantly comprehensively, this inhibitory action is stronger.α-mannatide and vitamin C are bred the inhibitory action that also demonstrates to a certain degree to the melanocyte of In vitro culture, but effect is not remarkable.This shows that five kinds of compound granules of the present invention significantly are better than single with α-mannatide and vitamin C to the melanocyte inhibition of proliferation effect of In vitro culture.
2.2 the synthetic measurement result of melanocyte adds all obviously decline (P＜0.01) of ability of the synthetic melanocyte of experimental group of five kinds of compound granules of the present invention, melanocyte has the prescription dependency to five kinds of compound granules of the present invention, along with five kinds of compound granules prescription of the present invention comprehensively, melanocyte is synthetic to descend gradually.α-mannatide and vitamin C are to the synthetic inhibitory action that also demonstrates to a certain degree of melanocyte, but effect is not remarkable.This shows that five kinds of compound granules of the present invention significantly are better than single with α-mannatide and vitamin C to the synthetic effect that suppresses of melanocyte.
2.3 the measurement result of tyrosinase activity adds all obviously decline (P＜0.01) of activity of the experimental group tryrosinase of five kinds of compound granules of the present invention, the activity of tryrosinase has the prescription dependency to five kinds of compound granules of the present invention, along with five kinds of compound granules prescription of the present invention comprehensively, the activity of tryrosinase descends gradually.α-mannatide and vitamin C also demonstrate to a certain degree inhibitory action to the activity of tryrosinase, but effect is not remarkable.This shows that five kinds of compound granules of the present invention significantly are better than single with α-mannatide and vitamin C to the activity inhibition effect of tryrosinase.
The checking of looks improving and the skin nourishing electuary curative effect of the present invention: Lee, the woman, 32 years old, the lamellar brown spot appearred in 3 years causes gestation face, use over 3 years variously to remove the speckle product always, but poor effect.Take electuary of the present invention 10 days (course of treatment), the thin out and conscious health each side of facial colour spot color function is than before for well.Take again 1 the course of treatment facial colour spot color by the brown stain Huang till now for yellowish, add up three place's mottle diameters and narrow down to 2.1cm from 3cm respectively, 2.3cm narrow down to 1cm, 1.2cm narrow down to 0.7cm, and facial ruddy, the skin smooth softness, high resilience, the hair color and luster is vivid submissive, the sleep quality height, and the mental status is good.After continuing to follow the tracks of 4 courses of treatment, the facial colour spot complete obiteration, skin is ruddy delicate, does not have recurrence half a year.
Looks improving and the skin nourishing electuary large-scale crowd curative effect of the present invention statistics: looks improving and the skin nourishing electuary orally taken for curing of the present invention 368 routine nongenetic hyperpigmentation disease patients, obtain better curative effect, report as follows: totally 368 examples are participated in experimenter, male 110 examples, woman's 258 examples, men and women's ratio is 1: 2.4; Age is 30 examples below 14 years old, 15-20 year 60 examples, and 21-30 year 101 examples, 31-40 year 99 examples, 41-50 year 50 examples, 28 examples more than 51 years old are maximum with 15-40 year person.These patients are carried out etiological analysis, irritated 62 examples, cosmetics stimulate 58 examples, menoxenia 48 examples, Colophonium or coal tar or coal gas contact 46 examples, oral contraceptive 29 examples, gestation 25 examples, menopause endocrine regulation 24 examples, each 20 example of medicine for external use, thermostimulation, other (comprising vitamin deficiency, anaphylactogen, dust etc.) totally 56 examples.Criterion of therapeutical effect: observe in day, measure transformations such as pigment shape, size, color and luster and surrounding skin are delicate, ruddy, judge and divide 4 grades.Promptly cure: the pigmented spots complete obiteration, to observe and do not recur half a year, institute is with transference cure; Produce effects: pigmented spots disappear more than 50%, and observation non-pigment half a year increases phenomenon again, with transference cure; Effectively: pigmented spots disappear in below 50%, or pigment speckle color is thin out, and it is delicate or ruddy that surrounding skin changes: invalid: pigmentation does not have the variation of improvement before and after the treatment.After treating two courses of treatment, cure 51 examples, produce effects 73 examples, effective 108 examples, invalid 136 examples, total effective rate is 63.0%; After treating four courses of treatment, cure 196 examples, produce effects 24 examples, effective 63 examples, invalid 85 examples, total effective rate is 76.9%.
Claims (10)
1, a kind of electuary with effects of beautification and nourishing face is characterized in that: its composition mainly comprises α-mannatide 10-40%, regulates metabolic Chinese medicine 60-90%.
2, a kind of electuary with effects of beautification and nourishing face according to claim 1 is characterized in that: described composition includes α-mannatide 10-40%, regulates the Chinese medicine 10-60% of metabolic Chinese medicine 10-50%, oxidation and removing free radicals.
3, a kind of electuary with effects of beautification and nourishing face according to claim 1 is characterized in that: described composition includes α-mannatide 10-35%, regulates the Chinese medicine 10-50% of metabolic Chinese medicine 10-40%, oxidation and removing free radicals, suppresses the Chinese medicine 10-50% that melanin forms.
4, a kind of electuary with effects of beautification and nourishing face according to claim 1 is characterized in that: described composition includes α-mannatide 10-30%, regulates the Chinese medicine 10-40% of metabolic Chinese medicine 10-30%, oxidation and removing free radicals, suppresses Chinese medicine 10-50%, the Chinese medicine 10-60% of whitening function that melanin forms.
5, a kind of electuary with effects of beautification and nourishing face according to claim 1 is characterized in that: described composition includes α-mannatide 10-20%, regulates metabolic Chinese medicine 10-30%, the Chinese medicine 10-30% of oxidation and removing free radicals, suppress that Chinese medicine 10-40%, the Chinese medicine 10-50% of whitening function, QI invigorating that melanin forms are enriched blood, the Chinese medicine 10-40% of nourishing the liver and kidney.
6, a kind of electuary according to claim 1 with effects of beautification and nourishing face, it is characterized in that: the metabolic Chinese medicine of described adjusting has natural pollen, Ganoderma, Folium Mori, Flos Ginseng, Flos Rosae Rugosae; The Chinese medicine of oxidation and removing free radicals has Radix Ginseng, Fructus Schisandrae Chinensis, Radix Angelicae Sinensis, Cordyceps, green tea, Semen Ginkgo, Radix Salviae Miltiorrhizae, Cortex Magnoliae Officinalis, Fructus Jujubae, Radix Pseudostellariae, Semen Ziziphi Spinosae, Radix Rehmanniae; The Chinese medicine that suppresses melanin formation has: ligustrazine, Fructus Corni, Radix Angelicae Sinensis, Aloe; The Chinese medicine of whitening function has: the Flos Sophorae Immaturus, Radix Notoginseng, Rhizoma Chuanxiong, pink, Radix Scutellariae, the Radix Angelicae Dahuricae, Margarita; The Chinese medicine of QI invigorating has: Radix Ginseng, Radix Codonopsis, the Radix Astragali, the Rhizoma Atractylodis Macrocephalae, Poria; The Chinese medicine of enriching blood has: Radix Angelicae Sinensis, Radix Rehmanniae Preparata, Radix Polygoni Multiflori, Fructus Lycii, Colla Corii Asini, Rhizoma Polygonati, Caulis Spatholobi; The Chinese medicine of nourishing the liver and kidney has: Cordyceps, Herba Dendrobii, the Radix Paeoniae Alba, Fructus Corni, Fructus Mori, Fructus Ligustri Lucidi, Herba Ecliptae, Radix Rehmanniae Preparata, Radix Achyranthis Bidentatae, Radix Polygoni Multiflori, Semen Cuscutae, Fructus Psoraleae, Poria.
7, a kind of electuary with effects of beautification and nourishing face according to claim 1 is characterized in that: contain α-Lu polysaccharide peptide 400g, natural pollen 150g, Ganoderma 100g, Folium Mori 150g, Flos Ginseng 100g, Flos Rosae Rugosae 100g among the every 1000g of described electuary.
8, a kind of electuary with effects of beautification and nourishing face according to claim 1 is characterized in that: contain α-mannatide 350g, natural pollen 50g, Ganoderma 50g, Folium Mori 120g, Flos Ginseng 80g, Flos Rosae Rugosae 100g, Radix Ginseng 30g, Fructus Schisandrae Chinensis 20g, Radix Angelicae Sinensis 20g, Cordyceps 20g, green tea 25g, Semen Ginkgo 30g, Radix Salviae Miltiorrhizae 10g, Cortex Magnoliae Officinalis 10g, Fructus Jujubae 35g, Radix Pseudostellariae 10g, Semen Ziziphi Spinosae 20g, Radix Rehmanniae 20g among the every 1000g of described electuary.
9, a kind of electuary with effects of beautification and nourishing face according to claim 1 is characterized in that: contain α-mannatide 300g, natural pollen 50g, Ganoderma 50g, Folium Mori 50g, Flos Ginseng 50g, Flos Rosae Rugosae 100g, Radix Ginseng 30g, Fructus Schisandrae Chinensis 20g, Cordyceps 20g, green tea 25g, Semen Ginkgo 30g, Radix Salviae Miltiorrhizae 10g, Cortex Magnoliae Officinalis 10g, Fructus Jujubae 35g, Radix Pseudostellariae 10g, Semen Ziziphi Spinosae 20g, Radix Rehmanniae 20g, ligustrazine 30g, Fructus Corni 30g, Radix Angelicae Sinensis 50g, Aloe 60g among the every 1000g of described electuary.
10, a kind of electuary with effects of beautification and nourishing face according to claim 1 is characterized in that: contain α-mannatide 100g among the every 1000g of described electuary, natural pollen 50g, Ganoderma 30g, Folium Mori 20g, Flos Ginseng 30g, Flos Rosae Rugosae 50g, Fructus Schisandrae Chinensis 20g, Cordyceps 20g, green tea 25g, Semen Ginkgo 30g, Radix Salviae Miltiorrhizae 10g, Cortex Magnoliae Officinalis 10g, Fructus Jujubae 35g, Radix Pseudostellariae 10g, Semen Ziziphi Spinosae 20g, Radix Rehmanniae 20g, ligustrazine 30g, Radix Angelicae Sinensis 30g, Aloe 50g, Flos Sophorae Immaturus 20g, Radix Notoginseng 10g, Rhizoma Chuanxiong 10g, pink 20g, Radix Scutellariae 20g, Radix Angelicae Dahuricae 20g, Margarita 50g, Radix Ginseng 10g, Radix Codonopsis 10g, Radix Astragali 10g, Rhizoma Atractylodis Macrocephalae 10g, Poria 10g, Radix Angelicae Sinensis 10g, Radix Rehmanniae Preparata 10g, Radix Polygoni Multiflori 10g, Fructus Lycii 10g, Colla Corii Asini 10g, Rhizoma Polygonati 10g, Caulis Spatholobi 10g, Cordyceps 10g, Herba Dendrobii 10g, Radix Paeoniae Alba 10g, Fructus Corni 20g, Fructus Mori 10g, Fructus Ligustri Lucidi 10g, Herba Ecliptae 10g, Radix Rehmanniae Preparata 10g, Radix Achyranthis Bidentatae 10g, Radix Polygoni Multiflori 10g, Semen Cuscutae 10g, Fructus Psoraleae 10g, Poria 10g.
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| CN1089250C (en) * | 1998-11-27 | 2002-08-21 | 杨延珍 | Anticancer oral liquid contg. lipolytic enzyme |
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