CN102334601A - Guar meal fermented protein feed additive, preparation method and feed containing guar meal fermented protein feed additive - Google Patents
Guar meal fermented protein feed additive, preparation method and feed containing guar meal fermented protein feed additive Download PDFInfo
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Abstract
The invention provides a guar meal fermented protein feed additive. The content of crude protein in a guar meal fermented protein feed is 52-54wt%. The guar meal fermented protein feed additive also contains lactobacillus and saccharomyces cerevisiae, wherein the content of lactobacillus is 8.5*1010cfu/g and the content of saccharomyces cerevisiae is 4.6*106cfu/g. In comparison with the prior art, the invention has the following beneficial effects: the anti-nutritional factor oligosaccharide in guar meal can be almost completely degraded; the additive mainly contains micromolecular proteins and simultaneously contains a certain amount of lactobacillus plantarum, saccharomyces cerevisiae and streptococcus faecalis so as to obviously raise the nutritional value of the additive and promote animal digestion and absorption. The additive provided by the invention is added into laying hen mixed feed and used for a laying hen nursing experiment. The result shows that the additive can be used to obviously reduce feed-egg ratio, decrease production cost and raise economic benefit.
Description
Technical field
The present invention relates to a kind of fermentation protein feedstuff additive and preparation method thereof, belong to the microbial technique application.
Background technology
Protein feeds is one of necessary important source material of animal husbandry development.The cluster bean dregs of rice are as a kind of novel protein feeds raw material; The adverse effect that can alleviate that present domestic protein feed resource is not enough, the dregs of beans selling at exorbitant prices causes for feedstuff industry and animal husbandry, countries such as India, Pakistan and Iran are also using the cluster bean dregs of rice as protein feeds at present.
The cluster bean dregs of rice are the byproducts after cluster bean (Cyamopsis tetragonoloba) is extracted bean gum, and nutritive value is very high.The aminoacid ingredient that it contains and other composition are shown in table 1 and table 2.The crude protein content of the cluster bean dregs of rice is between 35-47.5%, and the same with all assorted dregs of rice, the relative molecular weight of protein is bigger; Amino acid imbalance; Non-digestible, digestibility is merely 50-80%, and the ANFs that mainly contains is trypsin ihhibitor and residual guar gum.The main component of guar gum is: galactomannans, and its content in the common cluster bean dregs of rice is 18-20%, just the someone studied report as far back as 1964, in feeding of broiler, fed the growth performance that the daily ration that contains 1% guar gum can influence fryer; The growth performance that contains the diet feeding group of 2% guar gum can only reach the 61-67% of control group.So under the not enzyme-added situation of the cluster bean dregs of rice, contain in the daily ration of the 5% cluster bean dregs of rice and can contain the very guar gum of high-load, can influence the growth performance of fryer so greatly.The crude fiber content of the cluster bean dregs of rice is also very high in addition, be unfavorable for digesting and assimilating, and its smell is more special, if being used in directly that pig goes up maybe be responsive, and through scent of still after the enzyme external digestion, but taste is light sweet.
Table 1 aminoacid ingredient
| Composition | Percentage (%) |
| My god (door) winter propylhomoserin | 4.56-5.79 |
| Threonine | 1.46-1.52 |
| Serine | 2.08-2.16 |
| Glutamic acid | 9.58-11.68 |
| L-lysine | 2.50-2.75 |
| Alanine | 1.76-2.02 |
| Valine | 1.68-2.07 |
| Isoleucine | 1.34-1.68 |
| Leucine | 2.62-3.15 |
| Tyrosine | 1.76-1.55 |
| Benzene-third-amine-acid | 1.95-2.09 |
| Lysine | 2.15-2.31 |
| Histidine | 1.21-1.45 |
| Arginine | 6.50-7.84 |
| Synthetic Cystine | 0-0.66 |
| Methionine | 0.55-0.63 |
| Proline | 1.54-2.05 |
Table 2 cluster bean dregs of rice component table
| Component | Percentage (%) |
| Native protein | 48.9-55.16 |
| Water-solubility protein | 31.10-33.00 |
| Natural fat | 6.75-7.00 |
| Natural fiber | 2.85-5.00 |
| Ash content | 5.42-5.98 |
| |
90 milligrams/100 grams |
| Calcium | 410 milligrams/100 grams |
| Carbohydrate | 27.43 gram/100 grams |
Summary of the invention
An object of the present invention is to be to provide a kind of cluster bean dregs of rice fermentation protein feedstuff additive, to solve non-digestible, the defective that digestibility is low of existing protein feed.
The present invention solves the technical scheme that its technical problem takes:
Cluster bean dregs of rice fermentation protein feedstuff additive; It is characterized in that; By weight percentage; Crude protein content is 52-54% in the said cluster bean dregs of rice fermentation protein feedstuff, also contains Lactobacillus plantarum (Lactobacillaceae) and saccharomyces cerevisiae (Saccharomycescerevisiae) in the said cluster bean dregs of rice fermentation protein feedstuff additive, and the content of said Lactobacillus plantarum is 8.5 * 10
10Cfu/g, the content of said saccharomyces cerevisiae are 4.6 * 10
6Cfu/g.
Also contain streptococcus fecalis (Streptococcus faecalis) in the said cluster bean dregs of rice fermentation protein feedstuff additive, the content of said streptococcus fecalis is 6.6 * 10
9Cfu/g.
Another object of the present invention is the preparation method who is to provide said cluster bean dregs of rice fermentation protein feedstuff additive, it is characterized in that, and be raw material with the cluster bean dregs of rice that pulverize or not process pulverizing, adopt probiotics fermention, may further comprise the steps:
(1) cluster bean dregs of rice process is pulverized or not process pulverizing, and carried out sterilization treatment;
(2) seed culture:
A. the seed culture of Lactobacillus plantarum and streptococcus fecalis:
The preparation seed culture medium;
From refrigerator, take out the freezing glycerine pipe bacterial classification of Lactobacillus plantarum and streptococcus fecalis, behind the active dissolution, get 2ml bacterium liquid respectively and insert respectively and be equipped with in the triangular flask of 100ml seed culture medium, leave standstill in 40 ℃ and cultivate 12h;
B. the cultivation of saccharomyces cerevisiae:
1h begins the activation of active dried saccharomyces cerevisiae before the inoculation: the consumption of yeast is 0.02% of a fermentation materials, the sugar aqueous solution with 2%, 40 ℃ of activation 1h;
(3) inoculation and fermentation: will cultivate in the said cluster bean dregs of rice that ripe Lactobacillus plantarum and saccharomyces cerevisiae seed liquor access step (1) makes; The inoculum concentration of said Lactobacillus plantarum is 5-20%; The inoculum concentration of said saccharomyces cerevisiae is 2-5%; Add running water and make that the water content of fermentation raw material is 50%, mixing is under anaerobic in 40 ℃ of constant temperature culture 72h.
Said preparation method's inoculation step comprises also streptococcus fecalis is inserted in the said cluster bean dregs of rice that the inoculum concentration of said streptococcus fecalis is 10-15%.
Said seed culture medium comprises 3% dregs of beans, 1% glucose, running water.
Another object of the present invention is to provide a kind of protein feed, it is characterized in that, by weight percentage, said protein feed contains the said additive of 3-5%.
Lactobacillus plantarum used in the present invention, saccharomyces cerevisiae, streptococcus fecalis are all purchased in Chinese industrial microorganism fungus kind preservation center, and the cluster bean dregs of rice that adopted can be bought from commercial sources and obtain.
The beneficial effect that the present invention has is: contain ANFs in the said additive hardly; ANFs oligosaccharides in the cluster bean dregs of rice can be by almost completely degraded; Contain high molecular weight protein in the said additive hardly, mainly contain the small molecular protein that is easy to digest, also contain a certain amount of Lactobacillus plantarum, saccharomyces cerevisiae, streptococcus fecalis in the said additive simultaneously; Thereby make the nutritive value of said additive be improved significantly, and help animal digestion and absorption.By weight percentage; Is that the ratio of 3-5% is added in the laying-hen compound feed and is used for laying hen and feeds experiment with said additive in addition; The result is illustrated in the laying-hen compound feed and adds said additive and can obviously reduce feedstuff-egg ratio, reduces production costs, and increases economic efficiency.
Description of drawings
Further specify the present invention below in conjunction with the accompanying drawing and the specific embodiment.
Fig. 1 is the SDS-PAGE collection of illustrative plates of the protein molecular weight size distribution of the said additive of the present invention of employing polyacrylamide gel electrophoresis (SDS-PAGE) assay determination.
Fig. 2 is the thin plate chromatography collection of illustrative plates of said additive of the present invention.
The curve map that the protein feed that Fig. 3 shows the additive according to the invention contain different content changes with the nursing time the influence of laying rate of laying hen.
The curve map that the protein feed that Fig. 4 shows the additive according to the invention contain different content changes with the nursing time the influence of total egg size.
The specific embodiment
Present embodiment is being to implement under the prerequisite with technical scheme of the present invention, and below in conjunction with specifically illustrating and provide detailed embodiment and process, but protection scope of the present invention is not limited to following embodiment.
Embodiment 1
1, with after the pulverizing of 200g cluster bean dregs of rice process, crosses 20 order analyses sieve, in 121 ℃ of sterilizations 30 minutes, cool off subsequent use then; Other gets the 200g cluster bean dregs of rice and sterilized 30 minutes at 121 ℃ without pulverizing directly, cools off subsequent use.
2, seed culture:
A, preparation seed culture medium: get 3% dregs of beans, 1% glucose and running water,, cool off subsequent use in 121 ℃ of sterilizations 30 minutes;
The cultivation of b, Lactobacillus plantarum and streptococcus fecalis: the freezing glycerine pipe bacterial classification that from refrigerator, takes out Lactobacillus plantarum and streptococcus fecalis; Behind the active dissolution; Get 2ml bacterium liquid respectively and insert respectively and be equipped with in the triangular flask of 100ml seed culture medium, leave standstill in 40 ℃ and cultivate 12h.
C, saccharomyces cerevisiae are cultivated:
1h begins the activation of active dried saccharomyces cerevisiae before the inoculation: the consumption of yeast is 0.02% of a fermentation materials, the sugar aqueous solution with 2%, 40 ℃ of activation 1h;
3, inoculation and fermentation
Bacterial classification combination and inoculum concentration by different are divided into four kinds of inoculation fermentation groups and obtain the different additives finished product:
First group; (inoculum concentration is 20%, 40ml), (inoculum concentration is 10% to saccharomyces cerevisiae, 20ml), (inoculum concentration is 20% to streptococcus fecalis with cultivating ripe Lactobacillus plantarum; 40ml) insert respectively in the cluster bean dregs of rice without pulverizing of the said sterilization treatment of step 1; Add an amount of running water and make that total moisture content is 40%, under anaerobic, obtain finished product after stirring in 40 ℃ of constant temperature culture 72h;
Second group; (inoculum concentration is 20% with cultivating ripe Lactobacillus plantarum; 40ml), (inoculum concentration is 10% to saccharomyces cerevisiae, 20ml) inserts respectively in the cluster bean dregs of rice without pulverizing of the said sterilization treatment of step 1, adds an amount of running water and makes that total moisture content is 40%; Under anaerobic, obtain finished product after stirring in 40 ℃ of constant temperature culture 72h;
The 3rd group; (inoculum concentration is 20% with cultivating ripe Lactobacillus plantarum; 40ml), (inoculum concentration is 10% to saccharomyces cerevisiae, 20ml) inserts respectively in the cluster bean dregs of rice (crossing 20 order analyses sieve) of the sterilization treatment of the said warp pulverizing of step 1, adds an amount of running water and makes that total moisture content is 40%; Under anaerobic, obtain finished product after stirring in 40 ℃ of constant temperature culture 72h;
The 4th group; (inoculum concentration is 10% with cultivating ripe Lactobacillus plantarum; 20ml), (inoculum concentration is 10% to saccharomyces cerevisiae, 20ml) inserts respectively in the cluster bean dregs of rice (crossing 20 order analyses sieve) of the sterilization treatment of the said warp pulverizing of step 1, adds an amount of running water and makes that total moisture content is 40%; Under anaerobic, obtain finished product after stirring in 40 ℃ of constant temperature culture 72h.
Set up control group simultaneously, promptly do not add any bacterial classification, only adding running water, to adjust the water content of the said cluster bean dregs of rice be 40%, under anaerobic, obtains the control group finished product in 40 ℃ of constant temperature culture 72h after stirring.
4, the said additive for preparing is detected and testing result and conclusion
(1) mensuration of pH value
Take by weighing said additive samples 10g, adding distil water 90ml behind the stirring 30min, uses corrected in advance pH meter to measure its pH value, and the result is as shown in table 1.
(2) mensuration of viable count (cfu)
After said additive samples suitably diluted with sterilized water, adopt the tilt-pour process counting, cultivate 48h in 40 ℃.Testing result is as shown in table 3.
The pH of the said additive samples of table 3 and the mensuration of cfu
Annotate: LAB representes Lactobacillus plantarum; FLQJ representes streptococcus fecalis; Y representes saccharomyces cerevisiae.
Result from table 3 can find out that the pH difference of all experimental group samples is little, all between 4.4-4.5, explain add streptococcus fecalis after, the pH range of decrease of fermentation materials is also not obvious.Along with the increase of inoculum concentration, pH slightly descends, and clump count increases, and explains that the combination bacterial classification that is inserted can grow on the solid state substrate of the cluster bean dregs of rice, and it is had certain assimilation.
(3) the protein molecular weight size distribution of said additive samples is measured
Additive samples after 70 ℃ of oven dry, is pulverized 20 mesh sieves, adopt polyacrylamide gel electrophoresis (SDS-PAGE) to detect the protein molecular weight size distribution, observed the degraded situation of albumen.(said polyacrylamide gel electrophoresis is referring to Shen Ping, Fan Xiurong, Li Guangwu. microbiology experiment [M]. and Beijing: Higher Education Publishing House, 1996.) result that obtains is as shown in Figure 1.Among Fig. 11 is first group of additive, and 2 is second group of additive, and 3 is the 3rd group of additive, and 4 is the 4th group of additive, and GM is the preceding cluster bean dregs of rice sample of fermentation.As can beappreciated from fig. 1; Protein molecular mainly is distributed between the 12-98kDa in the preceding cluster bean dregs of rice of fermentation; After the solid state fermentation processing; The macro-molecular protein of additive has obtained thorough degraded described in four experimental group, generates a certain amount of small molecular protein, and the protein band brightness that shows as on the gel disappears; Contrasting first group and second group can find out, it is identical with first group not add in second group of streptococcus fecalis the protein molecular palliating degradation degree; The SDS-PAGE collection of illustrative plates that contrasts first group, second group and the 3rd group, the 4th group can find out that the cluster bean dregs of rice are pulverized or do not pulverized behind overmulling bacterium solid state fermentation, and high molecular weight protein has obtained degraded fully; Can infer that in conjunction with experimental result in the past the main cause that influences the high molecular weight protein degraded in the cluster bean dregs of rice is a fermentation time.From this experimental result, the cluster bean dregs of rice are behind 40 ℃ of mixed bacterium anaerobic fermentation 72h, and high molecular weight protein wherein can be degraded fully.
(4) mensuration of the oligosaccharides of said additive samples
The thin plate chromatographic analysis is adopted in the detection of oligosaccharides: accurately take by weighing fermentation protein feedstuff sample 5g in the 250ml triangular flask, add the ethanolic solution of 50ml 80%, 70 ℃ of water-bath lixiviate 1h.Get the 2ml leaching liquor, the centrifugal 10min of 10000rpm gets supernatant point sample on silica gel plate, and the point sample amount is 5 μ l, in developping solution (normal propyl alcohol: acetate: water=1: 1: 0.1, volume ratio), launches after the point sample drying, is expanded to from 2cm place, silica gel plate forward position.Naturally after drying, and spray colour developing liquid (liquid that develops the color consists of: 1mL1-naphthols+10mL phosphoric acid+989ml ethanol) drenched to whole flat board, and the preservation of after 140 ℃ baking 5min develops the color down, taking pictures immediately.Adopt the thin plate chromatography to analyze the oligosaccharides in the said additive samples, the result is as shown in Figure 2.Among Fig. 21 is first group of additive, and 2 is second group of additive, and 3 is the 3rd group of additive, and 4 is the 4th group of additive, and GM is the preceding cluster bean dregs of rice sample of fermentation, and SBM is dregs of beans (as size of comparing oligosaccharides among the GM and a content).TLC collection of illustrative plates through the contrast protein raw materials can find out that the oligosaccharides before the fermentation in the cluster bean dregs of rice is mainly trisaccharide and disaccharides, and concentration is higher, and the brightness that shows as spot is brighter.Especially the content of trisaccharide will be apparently higher than the content of raffinose in the dregs of beans.Behind overmulling bacterium solid state fermentation, all trisaccharide and disaccharides are almost completely degraded, and the oligosaccharides degraded of the 3rd group and the 4th group is thorough than first group and second group, shows as immaculate vestige in the swimming lane, and is cleaner.The fermentation strain ANFs of in the solid ferment process of the cluster bean dregs of rice, degrading effectively is described.Can find out that in conjunction with experimental result in the past the size of fermentation time and bacterial classification combination and inoculum concentration is little to the degraded situation influence of oligosaccharides in the cluster bean dregs of rice.The cluster bean dregs of rice are behind 40 ℃ of mixed bacterium (Lactobacillus plantarum+yeast) anaerobic fermentation 48h, and oligosaccharides wherein just can be degraded fully.
(5) conclusion
Lactobacillus plantarum, saccharomyces cerevisiae and streptococcus fecalis all can grow on cluster bean dregs of rice solid state substrate, show as material pH and descend, and the clump count in the wet stock of fermentation back can reach 10
8More than the cfu/g;
The result shows that the oligosaccharides in the cluster bean dregs of rice is mainly trisaccharide and disaccharides, and concentration is higher from thin plate chromatography (TLC) collection of illustrative plates.After overmulling bacterium solid state fermentation was handled, all trisaccharide and disaccharides were almost completely degraded;
Gel electrophoresis spectrum (SDS-PAGE) is the result show, protein molecular mainly concentrates between the 31-66kDa in the cluster bean dregs of rice, and after handling through solid state fermentation, macro-molecular protein has obtained partly degraded; And the protein molecular of molecular weight between 31-50kDa fails to degrade fully; Through the adjustment fermentation time, promptly behind 40 ℃ of mixed bacterium (Lactobacillus plantarum+saccharomyces cerevisiae) anaerobic fermentation 72h, high molecular weight protein is wherein degraded fully.
Contain small molecular protein and a certain amount of probio in the protein raw materials after the fermentation, the nutritive value of protein raw materials is significantly improved.
Embodiment 2
To the protein feed that the has added said additive investigation that experimentizes, investigate the feed intake of said additive to the egg-laying peak laying hen, laying rate, egg is heavy, eggshell color, the influence of production targets such as the incidence of disease and death rate.
Said protein feed comprises mixed feed, by weight percentage, also contains the said additive of 3-5%.
Said mixed feed is 846s, and feed corporation,Ltd provides by the credit of source, Hai'an.
(1) experiment is divided into groups: the single-factor experimental design is adopted in experiment, chooses health, age in days that body weight is approximate and be 360 of Hainan ash laying hens in 32 weeks, is divided into three groups at random; Wherein one group is control group; Two groups is experimental group, every group of 120 chickens, and each test group is established three repetitions.The feed consumption that the principle of experimental design is followed experimental group and control group use equates.
(2) experiment place and time: credit birds, beasts and eggs Co., Ltd carries out in the chicken farm in the source, Jiangsu, and be 8 weeks experimental period.
(3) experiment is with feed ingredient and prescription
Control group: mixed feed (846s), be also referred to as layer diets in this article, wherein contain dregs of beans 17%, barley 9.7%;
Test one group: added the protein feed of 3% additive,, also contained dregs of beans 13%, barley 10.7% comprising mixed feed;
Test two groups: added the protein feed of 5% additive,, also contained dregs of beans 10.5%, barley 11.2% comprising mixed feed.
(4) experimental result and analysis
In 8 experimental periods in week, the additive of different additions is as shown in Figure 3 to the influence of laying rate of laying hen.As can be seen from Figure 3; In mixed feed, add 3% and 5% additive, the laying rate of two experimental group will be higher than control group in preceding 5 weeks, and especially the laying rate of 3% experimental group improves more obvious; Since the 5th week; 5% experimental group laying rate almost remains unchanged about 86%, but no conspicuousness difference (p＞0.05) between three groups, explains that in the daily ration of laying hen, adding 5% additive does not have the conspicuousness influence to the laying rate of laying hen.
The additive of different additions is as shown in Figure 4 to the influence of total egg size.As can be seen from Figure 4, along with the increase of the addition of additive in the layer diets, total egg size rises gradually, and to the 5th week reaching peak, total egg size of experimental group begins to descend afterwards, and the variation tendency of two experimental group is very approaching; The egg size of two experimental group is higher than control group; Show with statistical software SPSS significance analysis; Total egg size does not have conspicuousness difference (p＞0.05) in three groups, and total egg size that the addition that additive in the daily ration of laying hen be described reaches 5% pair of laying hen does not have conspicuousness to be influenced.
In 8 experimental periods in week, can find out that from table 4 additive of interpolation 3% and 5% all will be higher than control group to egg number and the average egg weight of laying hen in layer diets, explain that the said additive of interpolation does not have a negative impact to the growth performance of laying hen; Significance analysis shows, no matter is that egg number or average egg weight all do not make significant difference between three groups, explains that the addition of additive in the daily ration of laying hen can reach 5% at least.
The additives that add different amounts in table 4 layer diets are to the laying hen influence with average egg weight of laying eggs
1: all data are mean value ± SD in the table;
2: when feeding the experiment beginning, piece number of on average laying eggs of each experimental group laying hen maintains about 102 as far as possible, each test index basically identical of each group, and difference is not remarkable.
In 8 experimental periods in week, can find out that from table 5 3% experimental group laying rate will be higher than control group, the laying rate of 5% experimental group is a little less than control group; The feedstuff-egg ratio of 3% experimental group and 5% experimental group is all low than control group; The broken abnormal rate of two experimental group is a little more than control group.The additive of above analysis result explanation interpolation 5% in the daily ration of laying hen does not all have influence to lay eggs the total egg number of the average daily output of laying hen, laying rate, day total amount, an average egg size etc., and feedstuff-egg ratio descends, and has improved economic benefit.
Table 5 additive is to the influence of laying hen production performance
In 30 days experimental period, the price of deed, Economic and Efficiency Analysis have been carried out.Visible by table 6, under all identical situation of water, electricity and labour's expenditure, to compare through each difference of organizing the food consumption cost and the income of laying eggs, every chicken of 3% experimental group is overcharged 0.17 yuan of benefit than control group, and increasing income reaches 5.38%; The income of every chicken of 5% experimental group is a little less than control group, compares only to have descended 0.6%; Explanation has been added additive and can have been increased economic efficiency in the daily ration of laying hen.
Table 6 test chicken feed consumption rate, feedstuff-egg ratio and economic benefit are relatively
More than show and described basic principle of the present invention, principal character and advantage of the present invention.The technical staff of the industry should understand; The present invention is not restricted to the described embodiments; That describes in the foregoing description and the specification just explains principle of the present invention; The present invention also has various changes and modifications under the prerequisite that does not break away from spirit and scope of the invention, and these variations and improvement all fall in the scope of the invention that requires protection.The present invention requires protection domain to be defined by appending claims and equivalent thereof.
Claims (6)
1. cluster bean dregs of rice fermentation protein feedstuff additive; It is characterized in that; By weight percentage; Crude protein content is 52-54% in the said cluster bean dregs of rice fermentation protein feedstuff additive, also contains lactobacillus, saccharomyces cerevisiae in the said cluster bean dregs of rice fermentation protein feedstuff additive, and the content of said lactobacillus is 8.5 * 10
10Cfu/g, the content of said saccharomyces cerevisiae are 4.6 * 10
6Cfu/g.
2. cluster bean dregs of rice fermentation protein feedstuff additive according to claim 1 is characterized in that, also contains streptococcus fecalis in the said cluster bean dregs of rice fermentation protein feedstuff additive, and the content of said streptococcus fecalis is 6.6 * 10
9Cfu/g.
3. preparing the method for described cluster bean dregs of rice fermentation protein feedstuff additive, it is characterized in that, is raw material with the cluster bean dregs of rice that pulverize or not process pulverizing, adopts probiotics fermention, may further comprise the steps:
(1) cluster bean dregs of rice process is pulverized or not process pulverizing, and carried out sterilization treatment;
(2) seed culture:
A. the seed culture of lactobacillus and streptococcus fecalis:
The preparation seed culture medium;
From refrigerator, take out the freezing glycerine pipe bacterial classification of Lactobacillus plantarum and streptococcus fecalis, behind the active dissolution, get 2ml bacterium liquid respectively and insert respectively and be equipped with in the triangular flask of 100ml seed culture medium, leave standstill in 40 ℃ and cultivate 12h;
B. the cultivation of saccharomyces cerevisiae:
1h begins the activation of active dried saccharomyces cerevisiae before the inoculation: the consumption of yeast is 0.02% of a fermentation materials, the sugar aqueous solution with 2%, 40 ℃ of activation 1h;
(3) inoculation and fermentation: will cultivate in the said cluster bean dregs of rice that ripe Lactobacillus plantarum and saccharomyces cerevisiae seed liquor access step (1) makes; The inoculum concentration of said Lactobacillus plantarum is 5-20%; The inoculum concentration of said saccharomyces cerevisiae is 2-5%; Add running water and make that the water content of fermentation raw material is 50%, mixing is under anaerobic in 40 ℃ of constant temperature culture 72h.
4. method according to claim 1 is characterized in that, said preparation method's inoculation step comprises also streptococcus fecalis is inserted in the said cluster bean dregs of rice that the inoculum concentration of said streptococcus fecalis is 10-15%.
5. method according to claim 1 is characterized in that, said seed culture medium is made up of 3% dregs of beans, 1% glucose, running water.
6. a protein feed that contains cluster bean dregs of rice fermentation protein feedstuff additive as claimed in claim 1 is characterized in that, by weight percentage, said protein feed contains the said additive of 3-5%.
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| CN104140989A (en) * | 2013-05-10 | 2014-11-12 | 上海源耀生物股份有限公司 | Method for preparing fish soluble pulp protein and soybean meal mixed raw material through solid state fermentation of many strains |
| CN104140989B (en) * | 2013-05-10 | 2016-11-30 | 上海源耀生物股份有限公司 | A kind of multi-strain solid-state fermentation preparation fish molten slurry albumen and the method for bean cake mixing raw material |
| CN103385359A (en) * | 2013-08-07 | 2013-11-13 | 上海源耀生物科技有限公司 | Feeding attractant for piglets and production method thereof |
| CN103385359B (en) * | 2013-08-07 | 2014-09-24 | 上海源耀生物股份有限公司 | Feeding attractant for piglets and production method thereof |
| CN103652487A (en) * | 2013-12-11 | 2014-03-26 | 济南凯因生物科技有限公司 | Method for preparing sow feed through microbial fermentation |
| CN103652487B (en) * | 2013-12-11 | 2015-07-22 | 广西九翔农牧有限责任公司 | Method for preparing sow feed through microbial fermentation |
| CN105410364A (en) * | 2015-10-30 | 2016-03-23 | 湖北邦之德牧业科技有限公司 | Compound small peptide preparation technology and product and feeding protein raw materials thereof |
| CN110545672A (en) * | 2017-02-28 | 2019-12-06 | Cj第一制糖株式会社 | Method for producing fermented guar flour |
| EP3590353A4 (en) * | 2017-02-28 | 2020-08-12 | CJ Cheiljedang Corporation | Method for manufacturing fermented guar meal |
| CN111317060A (en) * | 2020-03-06 | 2020-06-23 | 杨小武 | Puffed guar meal and preparation process and application thereof |
| CN113812524A (en) * | 2021-09-18 | 2021-12-21 | 安徽省凤阳县御膳油脂有限公司 | Preparation method of sesame seed meal fermented feed |
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