CN101375703A - Method for producing fermented bean pulp with high content of lysine - Google Patents
Method for producing fermented bean pulp with high content of lysine Download PDFInfo
- Publication number
- CN101375703A CN101375703A CNA200710045287XA CN200710045287A CN101375703A CN 101375703 A CN101375703 A CN 101375703A CN A200710045287X A CNA200710045287X A CN A200710045287XA CN 200710045287 A CN200710045287 A CN 200710045287A CN 101375703 A CN101375703 A CN 101375703A
- Authority
- CN
- China
- Prior art keywords
- soybean meal
- dregs
- lysine
- beans
- fermented bean
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Fodder In General (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention relates to a method for producing high lysine fermented soybean meal. The method comprises the following steps: a, preparing a soybean meal raw material; b, adding water into the soybean meal raw material and stirring up uniformly to obtain aqueous soybean meal slurry; c, carrying out germicidal treatment for the aqueous soybean meal slurry obtained from step b; d, adding urea and ammonium sulphate into the aqueous soybean meal slurry treated through step c, and stirring up uniformly to obtain a soybean meal culture medium; e, accessing microbial strains capable of generating lysine into the soybean meal culture medium obtained from step d; f, conducting temperature control fermentation for the soybean meal culture medium treated through step e to obtain fermented soybean meal; and g, drying the fermented soybean meal to obtain the final product. The invention has the advantages that the preparation method brings out an apparent increase in the nutrient substances present in the fermented product with the content of soybean meal protein rising by more than 5%, and an obvious boost in the content of lysine, which increases to over 4.0% under the action of microzyme, from an initial 2.8%.
Description
Technical field
The present invention relates to technical field of biological fermentation, particularly a kind of production method of fermented bean pulp with high content of lysine.
Background technology
Amino acid is the base unit that constitutes protein.Protein contained in the feed can not directly be digested and assimilated by animal, must be through the effect of enzyme, protein is decomposed into amino acid after, could enter blood by digestive system, reformulate the protein of self in animal body again.Therefore, animal is to the needs of protein, in fact just to amino acid whose needs.
Amino acid can be divided into two classes, and a class is an essential amino acid, and another kind of is nonessential amino acid.Every amino acid that can synthesize voluntarily in animal body not necessarily absorbs from feed, is called nonessential amino acid.And can not synthesize in the body or synthetic quantity can not satisfy the nutritional need of animal, and be essential for growth, must from feed, supply with, be called essential amino acid.
Protein decomposes back amino acid in the common feedstuffs kind more than 20 approximately, and it is the needed essential amino acids of animal that 10 seed amino acids are wherein arranged.Limiting amino acid is meant those essential amino acids that can not satisfy the animal needs in feed or the diet.Their shortage has limited the utilization of other amino acid in the livestock and poultry body in the diet, thereby has reduced the nutritive value of whole diet protein.Usually the amino acid that lacks most in the feed becomes first limiting amino acid.
Lysine is the maximum essential amino acid of animal body requirement.Animal body fully can not oneself be synthetic, and the animal that particularly grows is to the requirement height of lysine, and the speed of growth is fast more, and growth pressure is high more, and the lysine that then needs is also many more.Content is lower in most of animal common feedstuffs raw materials, so be first or second limiting amino acid.Its effect is to improve a poor appetite, promote to grow; Promote the healing of wound, fracture etc.; Enhancing is to the resistance of various infectious diseases.
Soybean cake (dregs of rice) dregs of beans is the byproduct of the different processing modes of oils preparation industry with soya-bean cake.Dregs of beans is the accessory substance after extraction or precompressed extraction are got oil.Gross protein value is 43%～46%, and the processing technology of soya-bean cake is through the mechanical expression immersion oil, gross protein value is generally more than 40%, and lysine content is 2.4～2.8%.The content of lysine far can not satisfy the needs of growth of animal in the mixed feed that with the dregs of beans is the major protein source, need add lysine in addition.So the content that improves lysine in the dregs of beans has very large economic worth.
The report that utilizes dregs of beans to prepare lysine is also arranged at present, as the Chinese patent publication number is the disclosed L-fermenting lysine method of utilizing the acid-treated product of dregs of beans as organic nitrogen source of 1977048A, this method is included in before the preparation culture medium, and the pH value of the acid hydrolysis products of dregs of beans is adjusted to 2-4.Compare with other pH value, this pH value through regulating makes fermentation level and polysaccharide yield increase at least 8% and at least 7% of L-lysine respectively.
Summary of the invention
Technical problem to be solved by this invention is that the method by microbiological treatment improves the lysine content in the fermented bean dregs, to solve the existing low problem of bean-dregs feed lysine content.
Technical problem to be solved by this invention can be achieved through the following technical solutions:
A kind of production method of fermented bean pulp with high content of lysine may further comprise the steps:
A, preparation dregs of beans raw material;
B, in the dregs of beans raw material, add entry and stir moisture soybean meal slurry, expect that wherein water weight ratio is: 1:0.2～1.5;
C, the moisture soybean meal slurry that step b is obtained are carried out sterilization treatment, and sterilising temp is 60～125 ℃, and sterilization time is 20-60min; Naturally cool to normal temperature after the sterilization;
D, will add urea and ammonium sulfate through the moisture soybean meal slurry after the step c sterilization, each addition is the 0.1-1% of dregs of beans weight, and stir the dregs of beans compost;
E, insert the microorganism fungus kind can produce lysine in the dregs of beans compost that obtains through steps d, bacterial classification access amount is 5～15% of a dregs of beans weight;
F, the dregs of beans compost after step e inserts bacterial classification carry out temperature controlled fermentation and obtain fermented bean dregs, and wherein fermentation temperature is 28～45 ℃; Fermentation time is 60～96 hours;
G, will carry out dry finished product through the fermented bean dregs after the step f fermentation.
Microorganism fungus kind of the present invention is selected from a kind of in photosynthetic bacteria, lactic acid bacteria, saccharomycete, actinomyces or the bacillus.Wherein be preferably saccharomycete.
In the present invention, among the step b, the material water weight ratio of dregs of beans raw material and water is preferably: 1:1.2.
In the present invention, among the step c, sterilising temp is preferably 121 ℃.
In the present invention, among the step e, the access amount of bacterial classification is preferably 20%.
In the present invention, among the step f, fermentation temperature is preferably 30 ℃.
In the present invention, in the step g, drying mode is a rotary flashing drying.
Use the resulting fermented product nutriment of the present invention and be significantly improved, the dregs of beans protein content has improved more than 5%, and lysine content obviously improves: by saccharomycetic effect the lysine content in the dregs of beans is brought up to more than 4.0% by initial 2.8%.
Simultaneously, after dregs of beans is handled by biofermentation, make various antigenic components, ANFs in the dregs of beans effectively be reduced removal, the protein in the dregs of beans is decomposed to form the little peptide of a large amount of plants.The little peptide absorptivity of the plant of this no antigen height can be used as weanling pig, poult, shrimp crab, the good protein source of especially many high-grade economic animals.Applying of this product with reducing the dependence of feed industry to animal fodders such as fish meal greatly, produces huge economic benefit simultaneously.
The specific embodiment
Below in conjunction with specific embodiment, further illustrate the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in and limit the scope of the invention.The experimental technique of unreceipted actual conditions in the following example, usually according to normal condition, or the condition of advising according to manufacturer.Ratio and percentage are based on weight, unless stated otherwise.
Embodiment 1
Prepare dregs of beans raw material 100Kg; In raw material, add 30Kg water and stir; Sterilization, 125 ℃, 20min; Add 0.1Kg urea and 0.1Kg ammonium sulfate, mixing; Inoculation yeast bacterium 15Kg; Temperature controlled fermentation, fermentation temperature are 45 ℃, 60 hours time; Rotary flashing drying, finished product.
Embodiment 2
Prepare dregs of beans raw material 100Kg; In raw material, add 50Kg water and stir; Sterilization, 100 ℃, 30min; Add 0.5Kg urea and 0.5Kg ammonium sulfate; Inoculation yeast bacterium 10Kg; Temperature controlled fermentation, fermentation temperature are 30 ℃, 75 hours time; Rotary flashing drying, finished product.
Embodiment 3
Prepare dregs of beans raw material 100Kg; In raw material, add 150Kg water and stir; Sterilization, 90 ℃, 50min; Add 0.75Kg urea and 0.75Kg ammonium sulfate; Inoculation yeast bacterium 8Kg; Temperature controlled fermentation, fermentation temperature are 35 ℃, 80 hours time; Sun dehydration, finished product.
Embodiment 4
Prepare dregs of beans raw material 100Kg; In raw material, add 120Kg water and stir; Sterilization, 60 ℃, 60min; Add 1Kg urea and 1Kg ammonium sulfate; Inoculation yeast bacterium 5Kg; Temperature controlled fermentation, fermentation temperature are 35 ℃, 95 hours time; Rotary flashing drying, finished product.
Embodiment 5
Lysine content is measured
Adopting by reference international standard ISO 5510-1984 " the effective lysine assay method of feed " detects the lysine content of the prepared fermented bean dregs of embodiment 1-4 respectively, is respectively 4.2%, 4.5%, 4.6%, 5.0%.
All quote in this application as a reference at all documents that the present invention mentions, just quoted as a reference separately as each piece document.Should be understood that in addition those skilled in the art can make various changes or modifications the present invention after having read above-mentioned instruction content of the present invention, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
Claims (7)
1, a kind of production method of fermented bean pulp with high content of lysine is characterized in that, may further comprise the steps:
A, preparation dregs of beans raw material;
B, in the dregs of beans raw material, add entry and stir soybean meal slurry, expect that wherein water weight ratio is: 1:0.2～1.5;
C, the soybean meal slurry that step b is obtained are carried out sterilization treatment, and sterilising temp is 60～125 ℃, and sterilization time is 20-60min; Naturally cool to normal temperature after the sterilization;
D, will add urea and ammonium sulfate through the soybean meal slurry after the step c sterilization, each addition is the 0.1-1% of dregs of beans weight, and stir the dregs of beans compost;
E, insert the microorganism fungus kind can produce lysine in the dregs of beans compost that obtains through steps d, bacterial classification access amount is 5～15% of a dregs of beans weight;
F, the dregs of beans compost after step e inserts bacterial classification carry out temperature controlled fermentation and obtain fermented bean dregs, and wherein fermentation temperature is that 28～45 ℃, fermentation time are 60～96 hours;
G, will carry out dry finished product through the fermented bean dregs after the step f fermentation.
2, the production method of fermented bean pulp with high content of lysine according to claim 1 is characterized in that, described microorganism fungus kind is selected from a kind of in photosynthetic bacteria, lactic acid bacteria, saccharomycete, actinomyces or the bacillus.
3, the production method of fermented bean pulp with high content of lysine according to claim 1 is characterized in that, described microorganism fungus kind is a saccharomycete.
4, the production method of fermented bean pulp with high content of lysine according to claim 1 is characterized in that, among the step b, the material water weight ratio of dregs of beans raw material and water is: 1:1.2.
5, the production method of fermented bean pulp with high content of lysine according to claim 1 is characterized in that, among the step c, sterilising temp is 120 ℃.
6, the production method of fermented bean pulp with high content of lysine according to claim 1 is characterized in that, among the step f, fermentation temperature is 30 ℃.
7, the production method of fermented bean pulp with high content of lysine according to claim 1 is characterized in that, in the step g, drying mode is a rotary flashing drying.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA200710045287XA CN101375703A (en) | 2007-08-27 | 2007-08-27 | Method for producing fermented bean pulp with high content of lysine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA200710045287XA CN101375703A (en) | 2007-08-27 | 2007-08-27 | Method for producing fermented bean pulp with high content of lysine |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101375703A true CN101375703A (en) | 2009-03-04 |
Family
ID=40419561
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA200710045287XA Pending CN101375703A (en) | 2007-08-27 | 2007-08-27 | Method for producing fermented bean pulp with high content of lysine |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101375703A (en) |
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102334601A (en) * | 2010-07-15 | 2012-02-01 | 上海源耀生物科技有限公司 | Guar meal fermented protein feed additive, preparation method and feed containing guar meal fermented protein feed additive |
| CN102763769A (en) * | 2012-08-06 | 2012-11-07 | 广东希普生物科技股份有限公司 | Method for preparing lysine-rich fermented soybean meal |
| CN103340306A (en) * | 2013-06-22 | 2013-10-09 | 河南歌瑞农牧股份有限公司 | Additive for growing and fattening beef cattle, and preparation method thereof |
| CN104557154A (en) * | 2013-10-29 | 2015-04-29 | 中国海洋大学 | Method for producing amino acid fertilizer from soybean meal through solid-state fermentation |
| CN104839443A (en) * | 2015-05-28 | 2015-08-19 | 河南双成生物科技有限公司 | Production method of functional protein feed rich in four essential amino-acids |
| CN104839445A (en) * | 2015-05-28 | 2015-08-19 | 河南双成生物科技有限公司 | Production method of functional protein feed rich in two kinds of essential amino-acids |
| CN104839444A (en) * | 2015-05-28 | 2015-08-19 | 河南双成生物科技有限公司 | Production method of functional protein feed rich in three essential amino-acids |
| CN105211637A (en) * | 2014-06-25 | 2016-01-06 | 都江堰惠农生物技术有限责任公司 | Livestock biological feed nourishing additive agent and production method thereof |
| CN106343161A (en) * | 2016-08-30 | 2017-01-25 | 惠州市瑰拉科技有限公司 | Fermentation treatment process for bean pulp |
| CN106343153A (en) * | 2016-08-30 | 2017-01-25 | 惠州市瑰拉科技有限公司 | Peanut meal fermenting treatment method |
| CN106359851A (en) * | 2016-08-30 | 2017-02-01 | 惠州市瑰拉科技有限公司 | Fermentation treatment technology of feeding rapeseed meal |
| CN107927397A (en) * | 2017-12-14 | 2018-04-20 | 佛山市高明通威饲料有限公司 | A kind of weanling pig religion groove Feed Manufacturing fermented bean dregs and its processing method |
| CN108041267A (en) * | 2017-12-14 | 2018-05-18 | 佛山市高明通威饲料有限公司 | Anti- enteritis albumen powder and its processing method in a kind of piglet religion slot feed processing |
| CN108541824A (en) * | 2018-04-13 | 2018-09-18 | 安徽天邦生物技术有限公司 | A kind of high bioactivity fermented bean dregs and the preparation method and application thereof |
-
2007
- 2007-08-27 CN CNA200710045287XA patent/CN101375703A/en active Pending
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102334601A (en) * | 2010-07-15 | 2012-02-01 | 上海源耀生物科技有限公司 | Guar meal fermented protein feed additive, preparation method and feed containing guar meal fermented protein feed additive |
| CN102334601B (en) * | 2010-07-15 | 2013-04-17 | 上海源耀生物科技有限公司 | Guar meal fermented protein feed additive, preparation method and feed containing guar meal fermented protein feed additive |
| CN102763769A (en) * | 2012-08-06 | 2012-11-07 | 广东希普生物科技股份有限公司 | Method for preparing lysine-rich fermented soybean meal |
| CN102763769B (en) * | 2012-08-06 | 2013-08-07 | 广东希普生物科技股份有限公司 | Method for preparing lysine-rich fermented soybean meal |
| CN103340306A (en) * | 2013-06-22 | 2013-10-09 | 河南歌瑞农牧股份有限公司 | Additive for growing and fattening beef cattle, and preparation method thereof |
| CN104557154A (en) * | 2013-10-29 | 2015-04-29 | 中国海洋大学 | Method for producing amino acid fertilizer from soybean meal through solid-state fermentation |
| CN105211637A (en) * | 2014-06-25 | 2016-01-06 | 都江堰惠农生物技术有限责任公司 | Livestock biological feed nourishing additive agent and production method thereof |
| CN104839444A (en) * | 2015-05-28 | 2015-08-19 | 河南双成生物科技有限公司 | Production method of functional protein feed rich in three essential amino-acids |
| CN104839445A (en) * | 2015-05-28 | 2015-08-19 | 河南双成生物科技有限公司 | Production method of functional protein feed rich in two kinds of essential amino-acids |
| CN104839443A (en) * | 2015-05-28 | 2015-08-19 | 河南双成生物科技有限公司 | Production method of functional protein feed rich in four essential amino-acids |
| CN106343161A (en) * | 2016-08-30 | 2017-01-25 | 惠州市瑰拉科技有限公司 | Fermentation treatment process for bean pulp |
| CN106343153A (en) * | 2016-08-30 | 2017-01-25 | 惠州市瑰拉科技有限公司 | Peanut meal fermenting treatment method |
| CN106359851A (en) * | 2016-08-30 | 2017-02-01 | 惠州市瑰拉科技有限公司 | Fermentation treatment technology of feeding rapeseed meal |
| CN107927397A (en) * | 2017-12-14 | 2018-04-20 | 佛山市高明通威饲料有限公司 | A kind of weanling pig religion groove Feed Manufacturing fermented bean dregs and its processing method |
| CN108041267A (en) * | 2017-12-14 | 2018-05-18 | 佛山市高明通威饲料有限公司 | Anti- enteritis albumen powder and its processing method in a kind of piglet religion slot feed processing |
| CN108541824A (en) * | 2018-04-13 | 2018-09-18 | 安徽天邦生物技术有限公司 | A kind of high bioactivity fermented bean dregs and the preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101375703A (en) | Method for producing fermented bean pulp with high content of lysine | |
| CN101107970B (en) | Bion cow concentrated feed and method for making same | |
| CN1279835C (en) | Method of eliminating antinutrition fuctor in soya dreg using fermentation method | |
| CN103478405B (en) | Method used for preparing probiotic preparations by using vitamin B2 fermentation liquid waste | |
| CN104920806A (en) | Bran protein feed preparing method by using mixed bacteria multi-step fermentation | |
| CN105410410A (en) | Pig complete fermented feed and preparation method thereof | |
| CN102719505A (en) | Method for preparing vegetable protein small peptide by microoprganism fermentation | |
| CN104000021B (en) | The working method of a kind of vegetalitas fermented feed | |
| CN101589774A (en) | High-performance environment-friendly pig feed and preparation method thereof | |
| CN101791064A (en) | High-performance environment-friendly shrimp fodder and preparation method thereof | |
| CN101744149A (en) | High-performance environment-friendly cattle feed and preparation method thereof | |
| CN109744365A (en) | A kind of mulberry leaf fermented feed and preparation method thereof | |
| CN108552408A (en) | A kind of cattle feed and preparation method thereof made of vinasse | |
| CN105379947A (en) | Animal feed and preparation method thereof | |
| CN101843297A (en) | Biological fermentation process for improving protein content of cottonseed meal and detoxicating cottonseed meal | |
| CN102934737A (en) | Preparation method of protein feed | |
| CN101194668B (en) | Process for preparing blood meal biological modified peptide protein and application of the same | |
| CN106260589A (en) | A kind of method that recycling wheat bran prepares albumen feedstuff | |
| CN110301526A (en) | Complex micro organism fungicide and its method for preparing bioactive feed | |
| CN101632411B (en) | Method for preparing high-quality rapeseed protein containing conjugated linoleic acid by multi-strain fermentation | |
| CN105076690A (en) | Production method for fermented soybean meal high in lysine and methionine | |
| CN101653189A (en) | Natural plant small molecular group substance concentrate and production method thereof | |
| CN103168914B (en) | Preparation technology of microbial glycolysis active peptide containing haematochrome | |
| CN105724762A (en) | Biologically fermented sow feed and preparation method thereof | |
| CN115176898A (en) | Traditional Chinese medicine fermented feed for sheep and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C53 | Correction of patent for invention or patent application | ||
| CB02 | Change of applicant information |
Address after: 201316 Pudong New Area City, the town of Shanghai, the first floor of the barley Bay Industrial Zone, No. 118, No. 5842 Applicant after: SHANGHAI YUANYAO BIOLOGICAL CO., LTD. Address before: 201316 Shanghai Airlines Nanhui District Road No. 109 Applicant before: Shanghai Yuanyao Biotechnology Co., Ltd. |
|
| COR | Change of bibliographic data |
Free format text: CORRECT: APPLICANT; FROM: SHANGHAI YUANYAO BIOLOGICAL TECHNOLOGY CO., LTD. TO: SHANGHAI YUANYAO BIOTECHNOLOGY CO., LTD. |
|
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20090304 |