CN103205373A - Quality clostridium butyricum for breeding piglets - Google Patents
Quality clostridium butyricum for breeding piglets Download PDFInfo
- Publication number
- CN103205373A CN103205373A CN2013100826568A CN201310082656A CN103205373A CN 103205373 A CN103205373 A CN 103205373A CN 2013100826568 A CN2013100826568 A CN 2013100826568A CN 201310082656 A CN201310082656 A CN 201310082656A CN 103205373 A CN103205373 A CN 103205373A
- Authority
- CN
- China
- Prior art keywords
- clostridium
- clostridium butyricum
- butylicum
- feed
- butyricum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Abstract
Disclosed is quality clostridium butyricum for breeding piglets. The quality clostridium butyricum is capable of maintaining and regulating micro-ecological balance and improving immune functions of the piglets. The technical scheme includes that the clostridium butyricum HM-18 is preserved in the China General Microbiological Culture Collection Center on 29th October, 2012, and the preservation number of the clostridium butyricum is CGMCC No.6731. The invention further discloses an application method for the quality clostridium butyricum.
Description
Technical field
The present invention relates to a strain clostridium butylicum, specifically is that the good clostridium butylicum that suckling piglet is cultured that is used for microecosystem regulation balance, enhancing body immunologic function is kept in a strain.
Background technology
Along with improving constantly of constant development of economy and standard of living, people more and more recognize the basic meaning of " green ".But because the driving of economic interests, antibiotic use has begun to spread unchecked, and particularly uses in a large number in feed, the formation that causes normal microflora imbalance in the animal body and suppress organism immune response, and the serious superinfection that causes, even can cause death; Secondly, microbiotic residual in the livestock and poultry body and in the food such as meat, egg, milk can not make its deactivation fully through heat treated, and the people is edible this based food for a long time, may cause chronic poisoning etc.If in feed, use probiotics, will reduce antibiotic consumption greatly, solve the problem that microbiotic can't resolve, enhance productivity, advance to the direction of green agriculture.
Clostridium butyricum active bacteria formulation in the market is all from Japanese import, and price is higher, and clostridium butyricum has wide application prospect as medicine, protective foods, fodder additives and microbial fertilizer.If clostridium butyricum as fodder additives, can make our livestock products develop towards green direction, whole livestock industry and feed already there is profound significance.
The present invention is directed to above-mentioned problem, develop the good clostridium butylicum HM-18(Clostridium butyricum HM-18 that a kind of suckling piglet is cultured), feed behind the suckling piglet as fodder additives, can be in the suckling piglet enteron aisle antagonism animal pathogen, keep and the microecosystem regulation balance, the enhancing body immunologic function, and produce amylase, VITAMIN, promote animal digesting and assimilating and trophism feed.
Summary of the invention
The object of the present invention is to provide a strain to keep the good clostridium butylicum that suckling piglet is cultured that is used for microecosystem regulation balance, enhancing body immunologic function.
The present invention is achieved through the following technical solutions.
One strain is used for the good clostridium butylicum that suckling piglet is cultured, it is characterized in that: described clostridium butylicum HM-18(Clostridium butyricum HM-18) on October 29th, 2012, be Da Tun road, Chaoyang District, BeiJing, China city in the address, China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation, preserving number: CGMCC No.6731.
Clostridium butyricum HM-18 separates in the health pig enteron aisle, Clostridium butyricum HM-18 can be in animal intestinal the antagonism animal pathogen, keep and the microecosystem regulation balance, strengthen the immunologic function of animal, and produce amylase, VITAMIN, promote animal digesting and assimilating and trophism feed.
A kind of application of clostridium butylicum is characterized in that: be used for improving suckling piglet production performance and diarrhoea aspect.
A kind of activation of clostridium butylicum and cultivation, it is characterized in that: the Clostridium butyricum HM-18 of freezing preservation is inoculated in the GPY liquid nutrient medium, cultivate 20h down for 37 ℃ in temperature, so going down to posterity to cultivate obtains described activation Clostridium butyricum HM-18 bacterial classification 2-3 time; Described GPY liquid nutrient medium is composed as follows: peptone 10.0g, yeast extract paste 10.0g, glucose 6.0g, MgSO
47H
2O1.0g, K
2HPO
42.0g, MnSO
450mg adds 1L distilled water, regulates pH to 6.8,121 ℃ of sterilization 15min.
The using method of a kind of clostridium butylicum in feed is characterized in that: the bacterium powder after strain fermentation, freeze-drying, the pulverizing and basal diet are stirred stand-by, the clostridium butyricum active bacteria number reaches 1 * 10 in the feed
5Cfu/g.
Clostridium butyricum HM-18 provided by the invention has following positively effect:
Experimental result shows, adds 1 * 10 in the weanling pig feed
5The clostridium butylicum of cfu/g can significantly improve the speed of growth of weanling pig, has saved feed intake, thereby can inference, adds clostridium butyricum, can improve the economic benefit of weanling pig production.
Test-results shows, adds 1 * 10 in the weanling pig feed
5The clostridium butylicum of cfu/g can reduce the incidence of diarrhea of weaned piglets, and then can reduce the generation of intestines source property disease, improves feed digestibility, improves the pig starter feed utilization ratio.
Description of drawings
Accompanying drawing is that diarrhea of weaned piglets improves the situation synoptic diagram.
Embodiment
The invention will be further described below in conjunction with embodiment; Following embodiment is illustrative, does not limit protection scope of the present invention.
Experimental technique among the following embodiment if no special instructions, is ordinary method.
The good clostridium butylicum that suckling piglet is cultured that is used for microecosystem regulation balance, enhancing body immunologic function is kept in one strain, it is characterized in that: described clostridium butylicum HM-18Clostridium butyricum HM-18 is on October 29th, 2012, be Da Tun road, Chaoyang District, BeiJing, China city in the address, China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation, preserving number: CGMCC No.6731.
Clostridium butyricum HM-18 separates in the health pig enteron aisle, Clostridium butyricum HM-18 can be in animal intestinal the antagonism animal pathogen, keep and the microecosystem regulation balance, strengthen the immunologic function of animal, and produce amylase, VITAMIN, promote animal digesting and assimilating and trophism feed.
A kind of application of clostridium butylicum is characterized in that: be used for improving suckling piglet production performance and diarrhoea aspect.
A kind of activation of clostridium butylicum and cultivation, it is characterized in that: the Clostridium butyricum HM-18 of freezing preservation is inoculated in the GPY liquid nutrient medium, cultivate 20h down for 37 ℃ in temperature, so going down to posterity to cultivate obtains described activation Clostridium butyricum HM-18 bacterial classification 2-3 time; Described GPY liquid nutrient medium is composed as follows: peptone 10.0g, yeast extract paste 10.0g, glucose 6.0g, MgSO
47H
2O1.0g, K
2HPO
42.0g, MnSO
450mg adds 1L distilled water, regulates pH to 6.8,121 ℃ of sterilization 15min.
The using method of a kind of clostridium butylicum in feed is characterized in that: the bacterium powder after strain fermentation, freeze-drying, the pulverizing and basal diet are stirred stand-by, the clostridium butyricum active bacteria number reaches 1 * 10 in the feed
5Cfu/g.
The application of described clostridium butylicum aspect the weanling pig production performance, concrete grammar is as follows:
Testing program: select kind for long white and Large White hybridize 1 generations 28 20 of age in days healthy piglets of weaning, be divided into 2 groups at random, every group of 10 piglets, 35 days test periods; Control group fed typical case corn dregs of beans type basis day, test group is fed and is added the basal diet of clostridium butyricum.Basal diet and trophic level see Table 1.
Table 1 basal diet prescription and trophic level
Index determining: average daily ingestion amount: accurate weighing every day and record test group and control group feeding coal, the average daily ingestion amount of every piglet is calculated in weighing and record clout amount during off-test; Average daily gain: 8 of first day (beginnings) of test, the 35th day (end) morning claim piglet heavy on an empty stomach, the average daily gain of calculating every piglet; Material anharmonic ratio: calculate test group and control group material anharmonic ratio according to average daily ingestion amount and average daily gain; Diarrhea rate: observe piglet ight soil situation every day after on-test, record test group and control group diarrhoea piglet head, and a last number of times that diarrhoea takes place with every group of piglet calculates diarrhea rate divided by total raising number of times.
The experimental animal management: the test piglet adopts high bed to raise, semi-open pig house, and natural ventilation, the piglet free choice feeding, the nipple water dispenser is freely drunk water; Immunity and expelling parasite program are carried out synchronously by the pig farm routine.In time receiving tank external loss material is put back in the groove, observes at any time, records and search for food and healthy state.
Experiment 1: the effect of raising weanling pig production performance clostridium butylicum HM-18(Clostridium butyricum HM-18).
Select kind for long white and Large White hybridize 1 generations 28 20 of age in days healthy piglets of weaning, be divided into 2 groups at random, every group of 10 piglets, control group fed basal diet, test group is fed and is added the basal diet of clostridium butylicum, clostridium butyricum viable count 〉=1 * 10 in the feed
5Cfu/g, 35 days test periods.Wean suckling piglet production performance improvement situation sees Table 2.
Table 2 weanling pig production performance is improved situation
As seen from the experiment, add butyric acid shuttle shape gemma bar in the daily ration, the weanling pig speed of growth significantly improves than control group, and the average daily gain amount is 70 grams.
Test 2: clostridium butylicum HM-18(Clostridium butyricum HM-18) improve the effect of diarrhea of weaned piglets.
Select kind for long white and Large White hybridize 1 generations 28 20 of age in days healthy piglets of weaning, be divided into 2 groups at random, every group of 10 piglets.30 days test periods, test adopts basal diet to form and trophic level sees Table 1.Observe piglet ight soil situation every day after on-test, record test group and control group diarrhoea piglet head, and a last number of times that diarrhoea takes place with every group of piglet calculates diarrhea rate divided by total raising number of times.Test grice diarrhoea rate is seen accompanying drawing.
As seen from the experiment, add clostridium butylicum in the daily ration, the diarrhea of weaned piglets rate is significantly increased than control group, and diarrhea rate hangs down 34.7%.
Claims (4)
1. a strain is used for the good clostridium butylicum that suckling piglet is cultured, and it is characterized in that: described clostridium butylicum HM-18(Clostridium butyricum HM-18) separate obtaining from the chitling road; This bacteria strain has been preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, preserving number: CGMCC No.6731, preservation date are on October 29th, 2012.
2. the application of a clostridium butylicum is characterized in that: be used for improving suckling piglet production performance and diarrhoea aspect.
3. the activation of a clostridium butylicum and cultivation, it is characterized in that: the Clostridium butyricum HM-18 of freezing preservation is inoculated in the GPY liquid nutrient medium, cultivate 20h down for 37 ℃ in temperature, so going down to posterity to cultivate obtains described activation Clostridium butyricum HM-18 bacterial classification 2-3 time; Described GPY liquid nutrient medium is composed as follows: peptone 10.0g, yeast extract paste 10.0g, glucose 6.0g, MgSO
47H
2O1.0g, K
2HPO
42.0g, MnSO
450mg adds 1L distilled water, regulates pH to 6.8,121 ℃ of sterilization 15min.
4. the using method of a clostridium butylicum in feed is characterized in that: the bacterium powder after strain fermentation, freeze-drying, the pulverizing and basal diet are stirred stand-by, the clostridium butyricum active bacteria number reaches 1 * 10 in the feed
5Cfu/g.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310082656.8A CN103205373B (en) | 2013-03-15 | 2013-03-15 | Quality clostridium butyricum for breeding piglets |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310082656.8A CN103205373B (en) | 2013-03-15 | 2013-03-15 | Quality clostridium butyricum for breeding piglets |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103205373A true CN103205373A (en) | 2013-07-17 |
| CN103205373B CN103205373B (en) | 2014-09-03 |
Family
ID=48752782
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310082656.8A Active CN103205373B (en) | 2013-03-15 | 2013-03-15 | Quality clostridium butyricum for breeding piglets |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103205373B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106479924A (en) * | 2016-10-31 | 2017-03-08 | 湖北绿雪生物科技有限公司 | The preparation of a kind of Clostridium butyricum and clostridium butyricum active bacteria preparation and application |
| CN106819487A (en) * | 2017-03-10 | 2017-06-13 | 天津市畜牧兽医研究所 | A kind of low albumen mixed feed of piglet containing clostridium butyricum and application |
| CN106922987A (en) * | 2017-03-16 | 2017-07-07 | 安徽省新康饲料有限公司 | A kind of complete feed preparation method based on clostridium butyricum |
| CN107970446A (en) * | 2017-10-31 | 2018-05-01 | 广西壮族自治区兽医研究所 | Preventing piglet diarrhea height exempts from shell egg powder capsule and preparation method and application |
| CN115211492A (en) * | 2022-06-07 | 2022-10-21 | 圣道生物技术(山东)有限公司 | Stress relieving medicine for resisting stress and promoting growth of livestock and poultry and preparation method and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102226162A (en) * | 2011-05-23 | 2011-10-26 | 江苏省微生物研究所有限责任公司 | Preparation method and application of composite microbial feed additive |
-
2013
- 2013-03-15 CN CN201310082656.8A patent/CN103205373B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102226162A (en) * | 2011-05-23 | 2011-10-26 | 江苏省微生物研究所有限责任公司 | Preparation method and application of composite microbial feed additive |
Non-Patent Citations (5)
| Title |
|---|
| 刘婷婷 等: "谷氨酰胺与丁酸梭菌对断奶仔猪生长性能、免疫功能、小肠形态和肠道菌群的影响", 《动物营养学报》, vol. 23, no. 6, 31 December 2011 (2011-12-31), pages 998 - 1005 * |
| 张彩云 等: "酪酸芽孢杆菌对断奶仔猪生产性能和血清生化指标的影响", 《营养饲料》, vol. 45, no. 13, 31 December 2009 (2009-12-31), pages 43 - 45 * |
| 张金生 等: "酪酸菌对弱仔猪生长性能的影响", 《饲料工业》, vol. 30, no. 8, 31 December 2009 (2009-12-31) * |
| 王保正 等: "酪酸菌对猪生长特性的影响", 《饲料工业》, vol. 28, no. 16, 31 December 2007 (2007-12-31), pages 39 - 40 * |
| 邓斐月: "丁酸梭菌与谷氨酰胺对断奶仔猪生长性能的影响及其机理研究", 《中国优秀硕士学位论文全文数据库 农业科技辑》, no. 12, 15 December 2010 (2010-12-15) * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106479924A (en) * | 2016-10-31 | 2017-03-08 | 湖北绿雪生物科技有限公司 | The preparation of a kind of Clostridium butyricum and clostridium butyricum active bacteria preparation and application |
| CN106479924B (en) * | 2016-10-31 | 2018-06-12 | 湖北绿雪生物科技有限公司 | The preparation and application of a kind of clostridium butyricum and clostridium butyricum active bacteria preparation |
| CN106819487A (en) * | 2017-03-10 | 2017-06-13 | 天津市畜牧兽医研究所 | A kind of low albumen mixed feed of piglet containing clostridium butyricum and application |
| CN106922987A (en) * | 2017-03-16 | 2017-07-07 | 安徽省新康饲料有限公司 | A kind of complete feed preparation method based on clostridium butyricum |
| CN106922987B (en) * | 2017-03-16 | 2018-08-07 | 安徽省新康饲料有限公司 | A kind of complete feed preparation method based on clostridium butyricum |
| CN107970446A (en) * | 2017-10-31 | 2018-05-01 | 广西壮族自治区兽医研究所 | Preventing piglet diarrhea height exempts from shell egg powder capsule and preparation method and application |
| CN115211492A (en) * | 2022-06-07 | 2022-10-21 | 圣道生物技术(山东)有限公司 | Stress relieving medicine for resisting stress and promoting growth of livestock and poultry and preparation method and application thereof |
| CN115211492B (en) * | 2022-06-07 | 2024-04-19 | 圣道生物技术(山东)有限公司 | Stress-resistant growth-promoting stress-relieving medicine for livestock and poultry, and preparation method and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103205373B (en) | 2014-09-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102168045B (en) | Bacillus subtilis preparation and preparation method thereof | |
| CN101580799B (en) | Micro-ecological preparation and application thereof | |
| CN103039699B (en) | Mixed fermentation feed and preparation method and application method of mixed fermentation feed | |
| CN102204632B (en) | Microbial feed additive for increasing livestock and poultry product quality and improving meat egg milk flavor and preparation method thereof | |
| CN102226162B (en) | Preparation method and application of composite microbial feed additive | |
| CN101463329B (en) | Saccharomyces cerevisiae, yeast preparation including the same, feed, and preparation and use thereof | |
| CN107173522A (en) | A kind of fermented feed containing probiotics and its preparation method and application | |
| CN103966124A (en) | Compound microecological preparation and additive, as well as premix thereof | |
| CN104472879A (en) | Tea residue feed additive and preparation method thereof | |
| CN105475624A (en) | Microbial fermentation feed as well as production method and application thereof | |
| CN103798540A (en) | Multi-strain microorganism pig feed additive and production method thereof | |
| CN104397323A (en) | Microbial feed additive | |
| CN103981118A (en) | Bacillus subtilis feed additive and preparation method and application thereof | |
| CN103181461A (en) | Composite microecological preparation for feed and preparation method thereof | |
| CN101974463B (en) | Lactobacillus reuteri and composite viable bacteria preparation thereof | |
| CN103205373B (en) | Quality clostridium butyricum for breeding piglets | |
| CN101946850A (en) | Lactobacillus reuteri fermented liquid feed, preparation method and application thereof | |
| CN103907749A (en) | Biological fermentation active carrier type composite premix feed as well as preparation method and application thereof | |
| CN103205375B (en) | Quality clostridium butyricum for breeding egg-laying poultry | |
| CN101971920A (en) | Porcine lactobacillus reuteri lyophilized preparation and preparation method thereof | |
| CN103181460A (en) | Preparation method and application of lactobacillus plantarum for feed | |
| CN103255080B (en) | Excellent bacillus coagulans for breeding piglets and application thereof | |
| CN111647541A (en) | Clostridium butyricum viable bacteria preparation, production method thereof and animal feed additive | |
| CN101933558B (en) | Compound microecological preparation and application thereof | |
| CN103255082B (en) | One plant of excellent bacillus coagulans and its application for growing and fattening pigs cultivation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |