Preventing piglet diarrhea height exempts from shell egg powder capsule and preparation method and application
Technical field
The invention belongs to veterinary medicine technical field, more particularly to a kind of preventing piglet diarrhea height to exempt from shell egg powder capsule and its system
Preparation Method and application.
Background technology
Newborn piglet diarrheic syndromes (NPDS) are the illnesss occurred frequently occurred under the conditions of current intensive pig production, particularly firm
Grice diarrhoea once occurs for the piglet of birth, its incidence may be up to 50%~80%, and the death rate is more up to 80%~
100%.Cause the cause of disease of grice diarrhoea varied, wherein it is most commonly seen with bacterial infection and viral infection, grind at present
Study carefully confirmation production enterotoxin escherichia coli (ETEC), Porcine epidemic diarrhea virus (PEDV), transmissible gastro-enteritis virus
(TGEC) and rotavirus (RV) is the main cause of disease for causing grice diarrhoea, and clinically they usually exist with mixed infection.
NPDS usually triggers epidemic spreads, is mainly prevented and treated at present by vaccine immunity and antibiotic, although vaccine immunity
To a certain extent can effectively prevention and control NPDS, but since the NPDS causes of disease are complicated, plus NPDS vaccines because a variety of technical barriers are difficult to dash forward
It is broken, cause NPDS immune effect of vaccine unsatisfactory, and antibiotic is invalid to virus diarrhea, and largely produced using antibiotic
Drug resistance and easily residual, therefore be highly desirable to find a kind of effective NPDS prevention and control treatment means.
Preventing piglet diarrhea height exempts from dried whole-egg (201110094936.1 publication date 2012-12-19 of the patent No.) containing efficient anti-
Grice diarrhoea special yolk antibody (IgY), because its preparation is simple, security is good, the curative effect advantage such as clearly, is added as feed
Agent is with a wide range of applications in the prevention and treatment of swine disease.However, since Yolk antibody is a kind of biological activity protein,
Activity is administered orally easily by the acid in high intensity stomach and high protein enzyme environmental disruption, it is therefore necessary to which it is chemically modified
Or physical protection.Vitamin A is required a kind of liposoluble vitamin in animal body, has and promotes Production of Livestock and Poultry, improves body
Immunity, improve the effect such as animal products quality;But vitamin A is easily influenced by environmental conditions such as illumination, oxygen and accelerates to drop
Solution, makes its application effect be affected;Clostridium butyricum is a kind of new probiotics, is added frequently as animal probiotics preparation
It is added in the feed of livestock and poultry, can effectively keeps animal intestinal tract micropopulation normal and treat relevant animal disease, improve animal
Production performance, improve body's resistance to disease, function, the clostridium butyricum such as immunity mainly play a role in the enteron aisle of animal.
Encapsulation is the most popular method for carrying out physical protection to medicine or IgY in recent years, it is different by application
Material parcel, protect medicine or IgY to hide the destruction of hydrochloric acid in gastric juice, make its fixed point release, reach and improve curative effect and reduce bad anti-
The purpose answered.At present, it yet there are no that preventing piglet diarrhea height exempts from dried whole-egg and vitamin A and clostridium butyricum assembles parcel microcapsules and makees
It is applied to grice diarrhoea and the report for the treatment of for feed addictive.
The content of the invention
The technical problem to be solved in the present invention is to provide a kind of anti-piglet good with anti-hydrochloric acid in gastric juice, slow release effect, stability
Diarrhea height exempts from shell egg powder capsule and preparation method and application.
To solve above-mentioned technical problem, the present invention uses following technical scheme:
Preventing piglet diarrhea height exempts from shell egg powder capsule, and the core of microcapsules exempts from dried whole-egg, vitamin A by preventing piglet diarrhea height
Formed with clostridium butyricum.
The weight ratio that preventing piglet diarrhea height exempts from dried whole-egg, vitamin A and clostridium butyricum is 25: 1: 1.
The wall material of microcapsules is made of chitosan, polyvinylpyrrolidone and cornstarch.
Chitosan, polyvinyl pyrrolidone and cornstarch weight ratio are 1: 1: 7~10.
The core and wall material of microcapsules account for the 30%~60% of capsules weight, 40%~70% respectively.
Above-mentioned preventing piglet diarrhea height exempts from the preparation method of shell egg powder capsule, is made through low temperature spray drying.
Above-mentioned preventing piglet diarrhea height exempts from the preparation method of shell egg powder capsule, comprises the following steps:
(1) prepared by core;
Independently weighed preventing piglet diarrhea height by proportioning and exempted from dried whole-egg, vitamin A, clostridium butyricum bacterium mud and be mixed into core,
Even be dissolved in distilled water prepares core material solution, and the percent weight in volume of solution is 30%;
(2) prepared by wall material;
Chitosan, polyvinylpyrrolidone and cornstarch are independently weighed by proportioning and is mixed into wall material, and uniform dissolution is in steaming
Wall material solution is prepared in distilled water, the percent weight in volume of solution is 30~40%;
(3) prepared by microcapsules;
Core material solution is uniformly mixed with wall material solution by 1: 1 volume ratio, prepares mixing suspension;By mix suspending
Liquid, low-temp. spraying is carried out under the conditions of 140 DEG C~150 DEG C of import, 40~50mL/min of charging rate, 70 DEG C~80 DEG C of outlet temperature
Mist drying prepares microcapsules.
Preventing piglet diarrhea height exempts from dried whole-egg by following operation preparation:By tri- kinds of different serotypes large intestines of K88, K99 and 987P
Bacillus inactivated vaccine and PEDV, TGEV, RV (G types) three Attenuate vaccines carry out fundamental immunity to laying hen, after being spaced 14d and 30d, use phase
Same vaccine carries out the 2nd and the 3rd booster immunization, then uses PEDV-GXLZ strains inactivated vaccine (immunizing dose PEDV >=105.0/
The 4th booster immunization only) is carried out, detects PEDV, IgY the and Ecoli agglutination titers of TGEV collect high-immunity egg system when reaching requirement
Standby height exempts from dried whole-egg.
Clostridium butyricum bacterium mud presses following operation preparation:Clostridium butyricum strain is activated, prepares clostridium butyricum zymotic fluid, prepares fourth
Sour clostridium bacterium mud.
Above-mentioned preventing piglet diarrhea height exempts from the application of shell egg powder capsule, and microcapsules are fed together with feed, will before feeding
Microcapsules are mixed with creep feed or pig starter feed by 1: 100 mass ratio is applied to feeding piglet.
For the problem of newborn piglet diarrheic syndromes are occurred frequently, medication effect is bad, inventor designs and is prepared for one kind
Preventing piglet diarrhea height exempts from shell egg powder capsule, and the core of microcapsules exempts from dried whole-egg, vitamin A and butyric acid shuttle by preventing piglet diarrhea height
Bacterium forms.Accordingly, corresponding preparation method has been also set up --- low temperature spray drying technique.Present invention system is first by anti-piglet abdomen
Rush down height and exempt from dried whole-egg, vitamin A and clostridium butyricum mixture progress encapsulation, technique is simple, and gained microcapsules footpath grain is
Even, encystation is good, and intestinal sustained releasing works well, and Yolk antibody gross activity is maintained at more than 80%, and clostridium butyricum is minimum to be deposited
Motility rate is 62.3%.Experiment shows, the microcapsules of the present invention are made an addition in pig starter feed, can effectively prevent and treat by
Grice diarrhoea disease caused by ETEC, PEDV, TGEC and RV, effectively reduces grice diarrhoea rate, significantly improves intestines function of piglings,
Improve nutritional ingredient to digest and assimilate, improve piglet feed intake and daily gain, improve immunity, alleviate wean to piglet stress
Property, improves the production performance of piglet.Therefore, prevention and treatment with grice diarrhoea are applied using the present invention as feed addictive,
It is of great significance to the livestock and poultry cultivation of research safety green.
Embodiment
Embodiment 1
(1) by the tri- kinds of different serotypes strain Escherichia colis of K88, K99 and 987P activated respectively by 2% inoculum concentration
It is inoculated into martin's bouillon culture medium and carries out 37 DEG C of culture 24h to logarithm production period about 3 × 109CFU/ml is then routinely square
Method prepares Escherichia coli inactivated vaccine;
(2) the 10th generation PEDV-GXLZ strain is recovered and is inoculated with Vero cells and carry out PEDV propagation, reached to cytopathy
When 75%, by cell multigelation 3 times, 15min is centrifuged with 12000r/min, removes cell fragment, collect cell conditioned medium, measure
Its virus titer, and press TCID50About 105/ 0.1mL prepares PEDV inactivated vaccines.
(3) by the ETEC inactivated vaccines and PEDV TGEV of preparation, RV (G types) three Attenuate vaccines by 1mL/ and 0.5mL/
Two wings for injecting laying hen respectively carry out first time fundamental immunity, and 14d presses same vaccine dose, same injection site into
Second of booster immunization of row, 44d are carried out the booster immunization of third time by same vaccine dose and same injection site, the
74d carries out the booster immunization of the 4th time with PEDV inactivated vaccines.
(4) at interval of 7d after being immunized for the second time, collect egg and carry out PEDV, IgY the and Ecoli agglutination titers of TGEV are held
Continuous monitoring, works as PEDV, TGEV IgY potency 0D values reach 1.5, Ecoli agglutination titers and reach 28During the above, high-immunity egg system is collected
Standby height exempts from dried whole-egg.
(5) the clostridium butyricum strain activated is accessed into phosphoric acid buffer 37 DEG C of Anaerobic culturels of liquid culture medium by 3% inoculum concentration
24h is to logarithm production period about 1 × 109CFU/ml, 12 000r/min centrifugation 2min prepare clostridium butyricum bacterium mud.
(6) height is weighed for 25: 1: 1 by weight and exempt from dried whole-egg, vitamin A and clostridium butyricum, be dissolved in 55 DEG C of steamings
Stirring and dissolving in distilled water, it is 30% solution to be made into percent weight in volume, is core.
(7) chitosan, polyvinylpyrrolidone and cornstarch are weighed by weight 1: 1: 8, first by chitosan and poly- second
Alkene pyrrolidone is dissolved in stirring and dissolving in 80 DEG C of distilled water, then adds cornstarch, and stirring and emulsifying is uniform, is made into weight
Percent by volume is 30% solution, is wall material.
(8) it will be mixed, stirred by 1: 1 volume ratio after the core material solution obtained by (6) and (7) and wall material solution cooling
Uniformly make system emulsion dispersion homogeneous, solid content proportion is 30% in solution.
(9) by emulsion obtained by (8) in 140 DEG C of inlet air temperature, charging rate 50mL/min, drying time 5s, outlet temperature
It is spray-dried under the conditions of 70 DEG C of degree, rewinding mouth is collected the material after spray drying, is that the anti-piglet height of gained is exempted from
Shell egg powder capsule.
(10) after measured, the preventing piglet diarrhea height of the implementation case is exempted from anti-ETEC, PEDV, TGVE and RV IgY of dried whole-egg and is resisted
Body activity be respectively 100%, 85.3%, 87.1% and 85.7%, Yolk antibody gross activity more than 85%, deposit by clostridium butyricum
Motility rate is 68.6%, is 8.62% in the release rate of simulated gastric fluid 2h, simulated intestinal fluid 4h release rates are 81.57%.
Embodiment 2
(1)~(6) are same as Example 1
(7) chitosan, polyvinylpyrrolidone and cornstarch are weighed by weight 1: 1: 10, first by chitosan and poly- second
Alkene pyrrolidone is dissolved in stirring and dissolving in 80 DEG C of distilled water, then adds cornstarch, and stirring and emulsifying is uniform, is made into weight
Percent by volume is 40% solution, is wall material.
(8) it will be mixed, stirred by 1: 1 volume ratio after the core material solution obtained by (6) and (7) and wall material solution cooling
Uniformly make system emulsion dispersion homogeneous, solid content proportion is 35% in solution.
(9) by emulsion obtained by (8) in 150 DEG C of inlet air temperature, charging rate 40mL/min, drying time 5s, outlet temperature
It is spray-dried under the conditions of 80 DEG C of degree, rewinding mouth is collected the material after spray drying, is that the anti-piglet height of gained is exempted from
Shell egg powder capsule.
(10) after measured, the preventing piglet diarrhea height of the implementation case is exempted from anti-ETEC, PEDV, TGVE and RV IgY of dried whole-egg and is resisted
Body activity is respectively 100%, 80.9%, 84.5% and 82.7%, and Yolk antibody gross activity is more than 80%, clostridium butyricum survival
Rate is 62.3%, and the release rate of simulated gastric fluid 2h is 8.43%, and simulated intestinal fluid 4h release rates are 80.84%.
Embodiment 3
(1)~(6) are same as Example 1
(7) chitosan, polyvinylpyrrolidone and cornstarch are weighed by weight 1: 1: 8, first by chitosan and poly- second
Alkene pyrrolidone is dissolved in stirring and dissolving in 80 DEG C of distilled water, then adds cornstarch, and stirring and emulsifying is uniform, is made into weight
Percent by volume is 40% solution, is wall material.
(8) it will be mixed, stirred by 1: 1 volume ratio after the core material solution obtained by (6) and (7) and wall material solution cooling
Uniformly make system emulsion dispersion homogeneous, solid content proportion is 35% in solution.
(9) by emulsion obtained by (8) in 150 DEG C of inlet air temperature, charging rate 40mL/min, drying time 5s, outlet temperature
It is spray-dried under the conditions of 80 DEG C of degree, rewinding mouth is collected the material after spray drying, is that the anti-piglet height of gained is exempted from
Shell egg powder capsule.
(10) after measured, the preventing piglet diarrhea height of the implementation case is exempted from anti-ETEC, PEDV, TGVE and RV IgY of dried whole-egg and is resisted
Body activity is respectively 100%, 84.6%, 85.3% and 84.8%, and Yolk antibody gross activity is more than 84%, clostridium butyricum survival
Rate is 63.6%, is 8.46% in the release rate of simulated gastric fluid 2h, simulated intestinal fluid 4h release rates are 82.23%.
Application test
Two feeding management differences of selection (opposite to manage preferable A and manage poor B fields relatively), PETV positive pigs
Field carries out the application test that health grice diarrhoea height exempts from shell egg powder capsule.
(28 ± 2) age in days weanling pig 50 similar in each pig farm selection parity, weight, random point 5 groups, every group 10
Head, respectively tests I groups (addition 1% embodiment, 1 height exempts from shell egg powder capsule), experiment II groups (exempt from by addition 1% embodiment, 2 height
Shell egg powder capsule), experiment III groups (addition 1% embodiment, 3 height exempts from shell egg powder capsule), (addition 0.5% is high for experiment IV groups
Exempt from dried whole-egg), experiment V groups (blank control group).Test duration 28d, in two stages, the first stage for wean after 0~
14d, with reference to (1998) 3~5kg grower pigs feeding standards of NRC configuration basal diet, preceding 7d is grouped on Basic drawing by experiment
Add each preparation feeding, latter 7 days unified feeding basal diets.Second stage is 15~28d after wean, with reference to reference to NRC
(1998) 5~10kg grower pigs feeding standards configure basal diet, unified to feed basal diet.Record piglet excrement situation daily
(recorded according to 0~3 level Four, 0 grade-dry, 1 grade-soft, 2 grades-dilute, 3 grades-water sample, wherein 0 and 1 grade is normal, 2 grades and 3 grades are abdomen
Rush down), calculate diarrhea rate, diarrhea frequency, additionally feed intake, daily gain and the material growth performance index ratio such as meat are recorded and
Evaluation.
As a result as shown in Table 1 and Table 2, in the PERV positives pig farm of different management conditions, by the feeding experiment of 28 days,
On the relatively good A pig farms of management condition, each height exempt from shell egg powder capsule addition group without grice diarrhoea occur, management compared with
The B pig farms of difference, each height exempt from shell egg powder capsule addition group compared with control group and dried whole-egg directly addition group, diarrhea rate and diarrhea
Index significantly reduces, and even its course of disease of generation diarrhea is also shorter;In addition each height exempts from the micro- glue of dried whole-egg in two pig farms of A, B
The average daily feed intake of capsule addition group piglet and average daily gain are obvious compared with control group and height exempt from dried whole-egg directly addition group
Improve, feed-weight ratio reduces.As it can be seen that 1% dried whole-egg microcapsule formulation is added in weanling pig daily ration can effectively reduce grice diarrhoea
Rate, improves piglet daily gain, reduces feed-weight ratio.
1 preventing piglet diarrhea height of table exempts from preventive effect of the dried whole-egg to grice diarrhoea
2 preventing piglet diarrhea height of table exempts from influence of the dried whole-egg to weaned piglets