CN113812524A - Preparation method of sesame seed meal fermented feed - Google Patents
Preparation method of sesame seed meal fermented feed Download PDFInfo
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- CN113812524A CN113812524A CN202111097490.8A CN202111097490A CN113812524A CN 113812524 A CN113812524 A CN 113812524A CN 202111097490 A CN202111097490 A CN 202111097490A CN 113812524 A CN113812524 A CN 113812524A
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- saccharomyces cerevisiae
- lactobacillus plantarum
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- 235000003434 Sesamum indicum Nutrition 0.000 title claims abstract description 75
- 235000012054 meals Nutrition 0.000 title claims abstract description 72
- 241000207961 Sesamum Species 0.000 title claims abstract description 71
- 238000002360 preparation method Methods 0.000 title claims description 14
- 238000000855 fermentation Methods 0.000 claims abstract description 99
- 230000004151 fermentation Effects 0.000 claims abstract description 99
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 51
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims abstract description 51
- 240000006024 Lactobacillus plantarum Species 0.000 claims abstract description 28
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims abstract description 28
- 229940072205 lactobacillus plantarum Drugs 0.000 claims abstract description 28
- 238000004880 explosion Methods 0.000 claims abstract description 23
- 239000007788 liquid Substances 0.000 claims abstract description 22
- 238000004945 emulsification Methods 0.000 claims abstract description 8
- 238000000034 method Methods 0.000 claims abstract description 5
- 239000000463 material Substances 0.000 claims description 31
- 239000002245 particle Substances 0.000 claims description 31
- 239000000839 emulsion Substances 0.000 claims description 26
- 238000011081 inoculation Methods 0.000 claims description 20
- 238000002156 mixing Methods 0.000 claims description 20
- 239000000203 mixture Substances 0.000 claims description 14
- 230000003749 cleanliness Effects 0.000 claims description 11
- 238000012258 culturing Methods 0.000 claims description 10
- 238000011049 filling Methods 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 239000008213 purified water Substances 0.000 claims description 9
- 238000003860 storage Methods 0.000 claims description 7
- 238000007789 sealing Methods 0.000 claims description 6
- 238000005422 blasting Methods 0.000 claims description 5
- 239000011521 glass Substances 0.000 claims description 5
- 235000016709 nutrition Nutrition 0.000 abstract description 4
- 239000002994 raw material Substances 0.000 abstract description 4
- 239000000796 flavoring agent Substances 0.000 abstract description 2
- 235000019634 flavors Nutrition 0.000 abstract description 2
- 230000035764 nutrition Effects 0.000 abstract description 2
- 102000010911 Enzyme Precursors Human genes 0.000 abstract 1
- 108010062466 Enzyme Precursors Proteins 0.000 abstract 1
- 239000000047 product Substances 0.000 description 16
- 235000018102 proteins Nutrition 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 6
- 241001465754 Metazoa Species 0.000 description 4
- 244000000231 Sesamum indicum Species 0.000 description 4
- 240000006240 Linum usitatissimum Species 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000000835 fiber Substances 0.000 description 3
- 230000000813 microbial effect Effects 0.000 description 3
- 239000008159 sesame oil Substances 0.000 description 3
- 235000011803 sesame oil Nutrition 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 235000019750 Crude protein Nutrition 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 235000004431 Linum usitatissimum Nutrition 0.000 description 2
- 108010064851 Plant Proteins Proteins 0.000 description 2
- 239000003674 animal food additive Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000003921 oil Substances 0.000 description 2
- 235000019198 oils Nutrition 0.000 description 2
- 238000004806 packaging method and process Methods 0.000 description 2
- 235000021118 plant-derived protein Nutrition 0.000 description 2
- 241000272525 Anas platyrhynchos Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 241000286209 Phasianidae Species 0.000 description 1
- 241000282898 Sus scrofa Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 125000003275 alpha amino acid group Chemical group 0.000 description 1
- 235000021120 animal protein Nutrition 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 239000008157 edible vegetable oil Substances 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000000944 linseed oil Substances 0.000 description 1
- 235000021388 linseed oil Nutrition 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000008239 natural water Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 108010027322 single cell proteins Proteins 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Animal Husbandry (AREA)
- Health & Medical Sciences (AREA)
- Food Science & Technology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Sustainable Development (AREA)
- Botany (AREA)
- Mycology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides a method for preparing sesame seed meal fermented feed, which takes sesame seed meal as a raw material and prepares the sesame seed meal fermented feed through crushing, steam explosion, emulsification, preparing zymogen liquid and two-stage fermentation. The invention utilizes the facultative anaerobic characteristic of saccharomyces cerevisiae and the strict anaerobic characteristic of lactobacillus plantarum to carry out two-stage fermentation, so that the sesame seed meal fermented feed has the nutrition and flavor of saccharomyces cerevisiae and lactobacillus plantarum.
Description
Technical Field
The invention belongs to the technical field of feed processing, and relates to a preparation method of a sesame seed meal fermented feed.
Background
Sesame, also known as flax or flax (the scientific name: Sesamum indicum), is a seed of the annual upright herbaceous plant flax. Sesame has an oil content of 55% and is an important edible oil source in China, and the squeezed oil is called sesame oil, linseed oil and sesame oil and is characterized by mellow smell and high nutritional value. The sesame seed meal is a byproduct of sesame oil extraction, the main component of the sesame seed meal is sesame protein, the content of crude protein of the sesame seed meal reaches 40-50%, the composition of amino acid is similar to that of bean meal with equal protein content, and the sesame seed meal is rich in amino acid required by various animal organisms and is a high-nutrition plant protein resource.
The protein feed is a single feed or a compound feed with natural water content of less than 45%, crude fiber of less than 18% and crude protein content of 20% or more. Protein feeds can be divided into four categories, namely plant protein feeds, animal protein feeds, single-cell protein feeds and non-protein nitrogen feeds according to different main sources. Sesame seed meal is a protein feed with high nutritional value, but the use of the sesame seed meal in compound feed is influenced due to high crude fiber content, poor palatability and the like.
The fermented feed takes microorganism and complex enzyme as biological feed starter strains, and the feed raw materials are converted into microbial mycoprotein, bioactive small peptide amino acid, microbial active probiotics and complex enzyme preparation as an integrated biological fermented feed. The product not only can make up amino acid which is easy to be lacked in conventional feed, but also can quickly convert the nutrient components of other raw feed materials, thereby achieving the effect of enhancing digestion, absorption and utilization. In the feed additive variety catalog released by the Ministry of agriculture in 2013 in China, microbial varieties allowed to be used in feed production are specified, and lactobacillus plantarum and saccharomyces cerevisiae belong to microbes allowed to be used in feed production.
Disclosure of Invention
The invention aims to provide a method for preparing a sesame seed meal fermented feed.
In order to achieve the above objects and other related objects, the present invention provides the following technical solutions: a preparation method of a sesame seed meal fermented feed comprises the following steps:
step 1: crushing sesame seed meal into sesame seed meal particles with the fineness of 20-40 meshes;
step 2: placing the crushed sesame seed meal particles in steam explosion equipment, and performing pressure maintaining treatment for 5-7min by using 2.0-2.5MPa steam; then closing the high-pressure steam and removing the steam pressure; collecting the materials after blasting treatment;
and step 3: mixing part of the sesame seed meal particles subjected to steam explosion treatment with purified water according to the mass ratio of 1:1.5-2.5, and then preparing into emulsion;
and 4, step 4: inoculating the saccharomyces cerevisiae into the emulsion according to the inoculation amount of 0.1-1.0%; putting the emulsion inoculated with the saccharomyces cerevisiae into a clean glass container, and culturing for 36-48h under the conditions that the cleanliness is higher than 1 ten thousand grade and the temperature is 28-30 ℃ to obtain a saccharomyces cerevisiae seed solution;
and 5: inoculating lactobacillus plantarum into the emulsion according to the inoculation amount of 0.25-1.0%; putting the emulsion inoculated with the lactobacillus plantarum into a clean anaerobic culture device, and culturing for 24-30h at the temperature of 37-40 ℃ to obtain lactobacillus plantarum seed liquid;
step 6: transferring the remaining sesame seed meal particles subjected to steam explosion treatment into a fermentation chamber, uniformly sprinkling the saccharomyces cerevisiae seed liquid on the sesame seed meal particles subjected to steam explosion treatment according to the inoculation amount of 0.25-1.5%, turning and uniformly mixing to obtain a saccharomyces cerevisiae fermentation material; stacking the saccharomyces cerevisiae fermentation material for fermentation, wherein the stacking slope is 30-45 degrees, and the stacking center height is 40-60 cm; keeping the cleanliness of the fermentation environment higher than 1 ten thousand grade and at the temperature of 25-30 ℃, and fermenting for 3-4 days to obtain a saccharomyces cerevisiae fermentation product;
and 7: uniformly sprinkling the lactobacillus plantarum seed liquid on the saccharomyces cerevisiae fermentation product according to the inoculation amount of 0.25-1.5%, turning over and uniformly mixing to obtain a fermentation material; filling the fermentation material into a fermentation barrel, filling and sealing the opening of the barrel; placing the sealed fermentation barrel filled with the fermentation material into a fermentation chamber, controlling the temperature of the fermentation chamber to be 33-37 ℃, and fermenting for 5-7d to obtain a second-stage fermentation product;
and 8: directly storing the fermentation barrel in a shady and cool environment without opening the cover after the second-stage fermentation is finished.
The preferable technical scheme is as follows: mixing the sesame seed meal particles with purified water, putting the mixture into emulsification equipment, treating the mixture for 15-30min at the rotation speed of 8000-.
The preferable technical scheme is as follows: in step 8, the storage environment is: storing at 0-6 deg.C for 21-28 days; storing for 7-10 days at 6-15 deg.C.
Due to the application of the technical scheme, compared with the prior art, the invention has the advantages that:
1. the invention utilizes the facultative anaerobic characteristic of saccharomyces cerevisiae and the strict anaerobic characteristic of lactobacillus plantarum to carry out two-stage fermentation, so that the sesame seed meal fermented feed has the nutrition and flavor of saccharomyces cerevisiae and lactobacillus plantarum.
2. The sesame seed meal is treated by steam explosion, so that the effect of killing bacteria is achieved, crude fibers in the sesame seed meal can be softened, and fermentation and metabolism of saccharomyces cerevisiae are facilitated.
Detailed Description
The following description of the embodiments of the present invention is provided for illustrative purposes, and other advantages and effects of the present invention will become apparent to those skilled in the art from the present disclosure.
Example 1: preparation method of sesame seed meal fermented feed
A method for preparing protein feed by using sesame seed meal is characterized by comprising the following technical steps.
(1) Crushing treatment of sesame seed meal
The sesame seed meal raw material should meet the requirements of GB/T22477-2008 sesame seed meal.
The sesame seed meal is processed by a pulverizer and pulverized into uniform sesame seed meal particles with the fineness of 20-40 meshes.
(2) Steam explosion treatment
Placing the crushed sesame seed meal particles in the step (1) in steam explosion equipment, and performing pressure maintaining treatment for 6min by using 2.2MPa steam; then, closing the high-pressure steam, and quickly removing the steam pressure; and collecting the materials after blasting treatment for later use.
(3) Emulsification
Taking the sesame seed meal particles subjected to steam explosion treatment in the part (2), mixing the sesame seed meal particles with food-grade purified water according to the mass ratio of 1:2, putting the mixture into emulsification equipment, stirring for 20min at the rotation speed of 10000r/min until the mixture is in an emulsified state, and collecting an emulsion for later use. Preferably, the emulsification operation is carried out in a room with a cleanliness higher than 1 ten thousand.
(4) Preparation of Saccharomyces cerevisiae seed liquid
The saccharomyces cerevisiae is a clean packaging dry product with the moisture content not higher than 6.0 percent, and meets the requirement of active dry yeast (saccharomyces cerevisiae) for feeding of GB/T22547-2008 feed additives.
Inoculating the saccharomyces cerevisiae into the emulsion in the step (3) according to the inoculation amount of 0.1-1.0%; and (3) putting the emulsion inoculated with the saccharomyces cerevisiae into a clean glass container, and naturally culturing for 42h under the conditions that the cleanliness is higher than 1 ten thousand grade and the temperature is 29 ℃ to obtain the saccharomyces cerevisiae seed solution. The obtained saccharomyces cerevisiae seed liquid is used for subsequent fermentation in time.
(5) Preparation of Lactobacillus plantarum seed solution
The lactobacillus plantarum is a clean packaging dry product with the water content not higher than 8.0 percent, and meets the requirements of' T/CSWSL 005-.
Inoculating lactobacillus plantarum into the emulsion in the step (3) according to the inoculation amount of 0.5%; and (3) putting the emulsion inoculated with the lactobacillus plantarum into a clean anaerobic culture device, and culturing for 26 hours at the temperature of 38 ℃ to obtain lactobacillus plantarum seed liquid. The obtained lactobacillus plantarum seed liquid is used for subsequent fermentation in time.
(6) First stage fermentation
And (3) taking the sesame seed meal particles subjected to steam explosion treatment in the part (2) for later use.
Transferring the sesame seed meal particles subjected to the steam explosion treatment in the step (2) into a fermentation chamber, uniformly sprinkling the saccharomyces cerevisiae seed liquid in the step (4) on the sesame seed meal particles according to the inoculation amount of 1%, turning and uniformly mixing to obtain a saccharomyces cerevisiae fermentation material; stacking the saccharomyces cerevisiae fermentation material for fermentation, wherein the stacking gradient is 35 degrees, and the stacking center height is 50 cm; keeping the cleanliness of the fermentation environment higher than 1 ten thousand grade, at the temperature of 27 ℃, and fermenting for 3.5 days to obtain the saccharomyces cerevisiae fermentation product.
(7) Second stage fermentation
Uniformly sprinkling the lactobacillus plantarum seed liquid in the step (5) on the saccharomyces cerevisiae fermentation product in the step (6) according to the inoculation amount of 0.8%, turning over and uniformly mixing to obtain a fermentation material; timely filling the fermentation material into a plastic fermentation barrel, filling and sealing the opening of the barrel; and (3) putting the sealed plastic fermentation barrel filled with the fermentation material into a fermentation chamber, controlling the temperature of the fermentation chamber to be 34 ℃, and fermenting for 6d to obtain a second-stage fermentation product. The plastic fermentation barrel is made of plastic with the sanitary requirement of the compound feed, and can realize good sealing after being covered; the capacity of the plastic fermentation barrel is suitably 20L.
The second stage of fermentation product is the sesame seed meal fermented feed.
(8) Storage and use
And (4) storing. The storage mode of the sesame seed meal fermented feed is that the plastic fermentation barrel after the second-stage fermentation in the step (7) is finished is directly stored in a cool environment without opening a cover; preferably, 25d can be stored in an environment at 2 ℃; in 10 ℃ environment, the storage time can be 8 d.
The preparation is used. Opening the plastic fermentation barrel after the second-stage fermentation in the step (7), taking out the sesame seed meal fermented feed, and directly feeding the animal; or mixing the sesame seed meal fermented feed with other feed raw materials according to a certain proportion, and feeding animals; particularly, when in use, the sesame seed meal fermented feed in the whole plastic fermentation barrel is used up at one time.
Preferably, the animal is suitable for feeding, including pig, chicken, duck, quail, etc.
Example 2: preparation method of sesame seed meal fermented feed
A preparation method of a sesame seed meal fermented feed comprises the following steps:
step 1: crushing sesame seed meal into sesame seed meal particles with the fineness of 20-40 meshes;
step 2: placing the crushed sesame seed meal particles in steam explosion equipment, and performing pressure maintaining treatment for 5min by using 2.0MPa steam; then closing the high-pressure steam and removing the steam pressure; collecting the materials after blasting treatment;
and step 3: mixing part of the sesame seed meal particles subjected to steam explosion treatment with purified water according to the mass ratio of 1:1.5, and then preparing into emulsion;
and 4, step 4: inoculating the saccharomyces cerevisiae into the emulsion according to the inoculation amount of 0.1 percent; putting the emulsion inoculated with the saccharomyces cerevisiae into a clean glass container, and culturing for 36h under the conditions that the cleanliness is higher than 1 ten thousand grade and the temperature is 28 ℃ to obtain saccharomyces cerevisiae seed liquid;
and 5: inoculating lactobacillus plantarum into the emulsion according to the inoculation amount of 0.25%; putting the emulsion inoculated with the lactobacillus plantarum into a clean anaerobic culture device, and culturing for 24 hours at the temperature of 37 ℃ to obtain lactobacillus plantarum seed liquid;
step 6: transferring the remaining sesame seed meal particles subjected to steam explosion treatment into a fermentation chamber, uniformly sprinkling saccharomyces cerevisiae seed liquid on the sesame seed meal particles subjected to steam explosion treatment according to the inoculation amount of 0.25%, turning and uniformly mixing to obtain a saccharomyces cerevisiae fermentation material; stacking the saccharomyces cerevisiae fermentation material for fermentation, wherein the stacking slope is 30 degrees, and the stacking center height is 40 cm; keeping the cleanliness of the fermentation environment higher than 1 ten thousand grade and the temperature of 25 ℃, and fermenting for 3d to obtain a saccharomyces cerevisiae fermentation product;
and 7: uniformly sprinkling the lactobacillus plantarum seed liquid on the saccharomyces cerevisiae fermentation product according to the inoculation amount of 0.25%, turning and uniformly mixing to obtain a fermentation material; filling the fermentation material into a fermentation barrel, filling and sealing the opening of the barrel; putting the sealed fermentation barrel filled with the fermentation material into a fermentation chamber, controlling the temperature of the fermentation chamber to be 33 ℃, and fermenting for 5d to obtain a second-stage fermentation product;
and 8: directly storing the fermentation barrel in a shady and cool environment without opening the cover after the second-stage fermentation is finished.
The preferred embodiment is: mixing the sesame seed meal particles with purified water, putting the mixture into emulsification equipment, treating the mixture for 15min at a rotating speed of 8000r/min until the mixture is in an emulsified state, and collecting the emulsified liquid.
The preferred embodiment is: in step 8, the storage environment is: storing at 0 deg.C for 28 deg.C; storing for 10 days in 6 ℃ environment.
Example 3: preparation method of sesame seed meal fermented feed
A preparation method of a sesame seed meal fermented feed comprises the following steps:
step 1: crushing sesame seed meal into sesame seed meal particles with the fineness of 40 meshes;
step 2: placing the crushed sesame seed meal particles in steam explosion equipment, and performing pressure maintaining treatment for 7min by using 2.5MPa steam; then closing the high-pressure steam and removing the steam pressure; collecting the materials after blasting treatment;
and step 3: mixing part of the sesame seed meal particles subjected to steam explosion treatment with purified water according to the mass ratio of 1:2.5, and then preparing into emulsion;
and 4, step 4: inoculating saccharomyces cerevisiae into the emulsion according to the inoculation amount of 1.0 percent; putting the emulsion inoculated with the saccharomyces cerevisiae into a clean glass container, and culturing for 48 hours under the conditions that the cleanliness is higher than 1 ten thousand grade and the temperature is 30 ℃ to obtain saccharomyces cerevisiae seed liquid;
and 5: inoculating lactobacillus plantarum into the emulsion according to the inoculation amount of 1.0%; putting the emulsion inoculated with the lactobacillus plantarum into a clean anaerobic culture device, and culturing for 30 hours at the temperature of 40 ℃ to obtain lactobacillus plantarum seed liquid;
step 6: transferring the remaining sesame seed meal particles subjected to steam explosion treatment into a fermentation chamber, uniformly sprinkling the saccharomyces cerevisiae seed liquid on the sesame seed meal particles subjected to steam explosion treatment according to the inoculation amount of 1.5%, turning and uniformly mixing to obtain a saccharomyces cerevisiae fermentation material; stacking the saccharomyces cerevisiae fermentation material for fermentation, wherein the stacking slope is 45 degrees, and the stacking center height is 60 cm; keeping the cleanliness of the fermentation environment higher than 1 ten thousand grade and the temperature of 30 ℃, and fermenting for 4d to obtain a saccharomyces cerevisiae fermentation product;
and 7: uniformly sprinkling the lactobacillus plantarum seed liquid on the saccharomyces cerevisiae fermentation product according to the inoculation amount of 1.5%, turning and uniformly mixing to obtain a fermentation material; filling the fermentation material into a fermentation barrel, filling and sealing the opening of the barrel; putting the sealed fermentation barrel filled with the fermentation material into a fermentation chamber, controlling the temperature of the fermentation chamber to be 37 ℃, and fermenting for 7d to obtain a second-stage fermentation product;
and 8: directly storing the fermentation barrel in a shady and cool environment without opening the cover after the second-stage fermentation is finished.
The preferred embodiment is: mixing the sesame seed meal particles with purified water, putting the mixture into emulsification equipment, treating the mixture for 30min at a rotating speed of 12000r/min until the mixture is in an emulsified state, and collecting the mixture to obtain an emulsion.
The preferred embodiment is: in step 8, the storage environment is: storing for 21d in an environment at 6 ℃; and storing for 7d in an environment at 15 ℃.
The foregoing is illustrative of the preferred embodiment of the present invention and is not to be construed as limiting thereof in any way, and any modifications or variations thereof that fall within the spirit of the invention are intended to be included within the scope thereof.
Claims (3)
1. A preparation method of a sesame seed meal fermented feed is characterized by comprising the following steps: comprises the following steps:
step 1: crushing sesame seed meal into sesame seed meal particles with the fineness of 20-40 meshes;
step 2: placing the crushed sesame seed meal particles in steam explosion equipment, and performing pressure maintaining treatment for 5-7min by using 2.0-2.5MPa steam; then closing the high-pressure steam and removing the steam pressure; collecting the materials after blasting treatment;
and step 3: mixing part of the sesame seed meal particles subjected to steam explosion treatment with purified water according to the mass ratio of 1:1.5-2.5, and then preparing into emulsion;
and 4, step 4: inoculating the saccharomyces cerevisiae into the emulsion according to the inoculation amount of 0.1-1.0%; putting the emulsion inoculated with the saccharomyces cerevisiae into a clean glass container, and culturing for 36-48h under the conditions that the cleanliness is higher than 1 ten thousand grade and the temperature is 28-30 ℃ to obtain a saccharomyces cerevisiae seed solution;
and 5: inoculating lactobacillus plantarum into the emulsion according to the inoculation amount of 0.25-1.0%; putting the emulsion inoculated with the lactobacillus plantarum into a clean anaerobic culture device, and culturing for 24-30h at the temperature of 37-40 ℃ to obtain lactobacillus plantarum seed liquid;
step 6: transferring the remaining sesame seed meal particles subjected to steam explosion treatment into a fermentation chamber, uniformly sprinkling the saccharomyces cerevisiae seed liquid on the sesame seed meal particles subjected to steam explosion treatment according to the inoculation amount of 0.25-1.5%, turning and uniformly mixing to obtain a saccharomyces cerevisiae fermentation material; stacking the saccharomyces cerevisiae fermentation material for fermentation, wherein the stacking slope is 30-45 degrees, and the stacking center height is 40-60 cm; keeping the cleanliness of the fermentation environment higher than 1 ten thousand grade and at the temperature of 25-30 ℃, and fermenting for 3-4 days to obtain a saccharomyces cerevisiae fermentation product;
and 7: uniformly sprinkling the lactobacillus plantarum seed liquid on the saccharomyces cerevisiae fermentation product according to the inoculation amount of 0.25-1.5%, turning over and uniformly mixing to obtain a fermentation material; filling the fermentation material into a fermentation barrel, filling and sealing the opening of the barrel; placing the sealed fermentation barrel filled with the fermentation material into a fermentation chamber, controlling the temperature of the fermentation chamber to be 33-37 ℃, and fermenting for 5-7d to obtain a second-stage fermentation product;
and 8: directly storing the fermentation barrel in a shady and cool environment without opening the cover after the second-stage fermentation is finished.
2. The method for preparing the sesame seed meal fermented feed according to claim 1, which is characterized in that: mixing the sesame seed meal particles with purified water, putting the mixture into emulsification equipment, treating the mixture for 15-30min at the rotation speed of 8000-.
3. The method for preparing the sesame seed meal fermented feed according to claim 1, which is characterized in that: in step 8, the storage environment is: storing at 0-6 deg.C for 21-28 days; storing for 7-10 days at 6-15 deg.C.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2024139079A1 (en) * | 2022-12-28 | 2024-07-04 | 天津博菲德科技有限公司 | Lactobacillus plantarum and use thereof in preparation of sesame meal with low phytic acid, high acid-soluble protein and high organic acid |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102334601A (en) * | 2010-07-15 | 2012-02-01 | 上海源耀生物科技有限公司 | Guar meal fermented protein feed additive, preparation method and feed containing guar meal fermented protein feed additive |
| CN103283961A (en) * | 2013-05-10 | 2013-09-11 | 浙江工业大学 | Method for preparing health-benefiting fermented soybean meal |
| CN103535511A (en) * | 2013-09-25 | 2014-01-29 | 江南大学 | Method for producing feed with rich peptide and rich prebiotics by fermenting high-temperature soybean meal |
| CN104839457A (en) * | 2015-05-28 | 2015-08-19 | 河南双成生物科技有限公司 | Method for preparing small peptide-enriched protein feed material based on steam explosion in combination with solid enzymatic hydrolysis |
| CN105010758A (en) * | 2015-06-30 | 2015-11-04 | 河南双成生物科技有限公司 | Method of producing protein feed by liquid-solid two-step fermentation method |
| CN111436526A (en) * | 2020-04-01 | 2020-07-24 | 浙江大学 | Preparation method and application of fermented rice bran meal with bacterium enzyme for improving growth performance of fattening pigs |
-
2021
- 2021-09-18 CN CN202111097490.8A patent/CN113812524A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102334601A (en) * | 2010-07-15 | 2012-02-01 | 上海源耀生物科技有限公司 | Guar meal fermented protein feed additive, preparation method and feed containing guar meal fermented protein feed additive |
| CN103283961A (en) * | 2013-05-10 | 2013-09-11 | 浙江工业大学 | Method for preparing health-benefiting fermented soybean meal |
| CN103535511A (en) * | 2013-09-25 | 2014-01-29 | 江南大学 | Method for producing feed with rich peptide and rich prebiotics by fermenting high-temperature soybean meal |
| CN104839457A (en) * | 2015-05-28 | 2015-08-19 | 河南双成生物科技有限公司 | Method for preparing small peptide-enriched protein feed material based on steam explosion in combination with solid enzymatic hydrolysis |
| CN105010758A (en) * | 2015-06-30 | 2015-11-04 | 河南双成生物科技有限公司 | Method of producing protein feed by liquid-solid two-step fermentation method |
| CN111436526A (en) * | 2020-04-01 | 2020-07-24 | 浙江大学 | Preparation method and application of fermented rice bran meal with bacterium enzyme for improving growth performance of fattening pigs |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2024139079A1 (en) * | 2022-12-28 | 2024-07-04 | 天津博菲德科技有限公司 | Lactobacillus plantarum and use thereof in preparation of sesame meal with low phytic acid, high acid-soluble protein and high organic acid |
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