CN102301923A - Method for raising seedlings of golden bough goldenrain tree by grafting - Google Patents

Method for raising seedlings of golden bough goldenrain tree by grafting Download PDF

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Publication number
CN102301923A
CN102301923A CN201110226203A CN201110226203A CN102301923A CN 102301923 A CN102301923 A CN 102301923A CN 201110226203 A CN201110226203 A CN 201110226203A CN 201110226203 A CN201110226203 A CN 201110226203A CN 102301923 A CN102301923 A CN 102301923A
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scion
stock
grafting
branch
cut
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CN201110226203A
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窦全琴
董筱昀
张敏
蒋泽平
蒋春
黄利斌
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Jiangsu Forestry Academy
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Jiangsu Forestry Academy
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Abstract

The invention discloses a method for raising seedlings of golden bough goldenrain tree by grafting. The method comprises the following steps: (1) selecting scions; (2) storing the scions; (3) making the scions; (4) selecting and cutting a tree stock; (5) jointing or inserting; and (6) managing grafted seedlings. The method for raising the seedlings by grafting disclosed by the invention has the advantages that: firstly, the problem of difficult vegetative propagation of the golden bough goldenrain tree can be solved; secondly, excellent ornamental property of leaves and branches of the golden bough goldenrain tree is maintained, and the growth adaptability and stress resistance of the golden bough goldenrain tree are improved; and thirdly, seedling formation speed is fast, the tree stock is available, and only a small segment of branch is taken as one scion, thus propagation period is short, propagation coefficient is high and massive seedlings can be obtained. The method for raising the seedling of golden bough goldenrain tree disclosed by the invention has important popularization value in rapid propagation of the ornamental nursery stock of a goldenrain tree.

Description

A kind of gold branch Luan Shu grafting seedlings-growing method
Technical field
The present invention relates to a kind of woody plant asexual reproduction method, specifically relate to a kind of gold branch Luan Shu grafting seedlings-growing method.
Background technology
Gold branch Luan Shu is a mutation of the natural bud mutation of Luan Shu (Koelreuteria integrifoliola), and its young leaves is orange colour, and autumn leaves is completely set golden yellow brilliance, and winter, branch also was golden yellow, had much ornamental value.In order to keep the distinctive ornamental value of gold branch Luan Shu, must use asexual reproduction method and breed its seedling, gold branch Luan Shu branch cutting and root are inserted the very difficulty of taking root all, and reproductive efficiency is low.Through gold branch Luan Shu grafting with the propagation method of Luan Shu as stock; The merit that can keep gold branch Luan Shuye and branch to view and admire; Improve the adaptability and the resistance of gold branch Luan Shu growth, shortened the breeding cycle, greatly improved the reproduction coefficient of golden branch Luan Shu.
Summary of the invention
In order to solve the quick breeding of gold branch Luan Shu, the object of the present invention is to provide a kind of easy and simple to handle, can keep good characteristic that a gold Luan Shu views and admires and the propagation method of rapid Cheng Miao.
The present invention realizes through following technical scheme:
A kind of gold branch Luan Shu grafting method is characterized in that it is made up of the following step:
(1) choosing of scion: winter to early spring is the clip branch on robust growth, the gold branch Luan Shu elite stand that do not sprout as yet, and branch is cut into 15~25cm, wraps up with wet cloth, and is subsequent use;
(2) storage of scion: place the bulk container that is filled with water to heat in the container of splendid attire paraffin; When treating water temperature rise to the 100 ℃ left and right sides; One in the branch of step (1) is immersed taking-up immediately in the paraffin that melts; Turn around again and dip in the other end, make the appearance of whole scion all cover the very thin paraffin of one deck, be placed on the low temperature place refrigeration about 5 ℃ then.Subsequent use;
(3) making of scion: the branch after will preserve spring is cut into the long scion of 5~8cm, and the scion bottom is whittled into wedge shape, and according to the rugosity of stock, when stock was thick, scion can be cut thickly slightly, and when stock was thin, scion can be cut thin.Subsequent use;
(4) selection of stock with cut anvil: 3~4cm cuts off at the place Luan Shu stock of will not sprout apart from ground, scabbles section, and the downward vertical cut at the edge of xylem will impose uniformity without examining individual cases, and kerf width and scion equal diameters reach 2~3cm deeply; Begun to sprout like stock, 3~4cm cuts off at the place apart from ground with stock, scabbles section and gets final product, and is for use;
(5) engage or insert: the stock of not sprouting can be used cut-grafting, step (3) scion inserting step (4) stock otch, uses the bottom-up whipping of plastic tape tight then; For the stock that begins to sprout, the stock cortex is vertically cut an osculum, scion is inserted from the stock otch along xylem and in the middle of the phloem, whips closely with plastic tape again.
(6) management of grafting: be carried out to biopsy after the grafting in 10~15 days, the bud in the scion keeps fresh or germination and growth then shows and become alive, and survival rate can reach 80~90%.In time wipe out branch and the root sprout tillers branch sprouted on the grafting stock, guarantee the nutrition supply and the growth of scion.The scion sprout growth is removed the bondage of plastic tape after 1~2 month.Should keep the moistening of field soil between the grafting healing stage, grafting can carry out management such as normal field weeding, fertilising after becoming to live.
Further, 1 year living diameter that the said scion of step (1) is taked in tree crown, clip growth in top is enriched is healthy and strong at 1~2.5cm, the full branch of sleeping bud;
The time of the said grafting of step (3) 15 days~March 25 March in spring the Luan Shu nursery stock do not sprout as yet or carry out when being about to sprout; Scion cut get be with the scion bottom from apart from the nearest back side, bud position of lower cut, with grafting knife parallel end that is cut to the scion axis downwards when inscribe reaches xylem, tangent plane is grown 3~5cm, subsequently scion bevel offside is splayed into 0.5~1.0cm inclined-plane.Scion cut thickness generally at 0.3~0.5cm;
The said stock of step (4) is selected 1~2 year living Luan Shu seedling of the leading thread 2~4cm of robust growth, no damage by disease and insect;
The mode of the said grafting of step (5) is the big bevel of scion inwards, inserts the stock otch, and is inserted into the end, makes scion aim at snap with the cambium of stock.If anvil, fringe do not wait slightly, can aim at cambium on one side; For beginning the stock of leading thread more than 2cm of sprouting; The stock cortex is vertically cut an osculum; Length is 1/2~2/3 of scion length, inserts facing to xylem the long bevel of scion from the stock otch along xylem and in the middle of the phloem, and makes the scion back side aim at stock otch center; White 0.5~1.0cm is stayed on bevel top during insertion, is beneficial to the healing of callus growth and otch.
Beneficial effect of the present invention: adopt grafting seedlings-growing method of the present invention to solve the vegetative problem of golden branch Luan Shu, the merit that keeps gold branch Luan Shuye and branch to view and admire improves the adaptability and the resistance of gold branch Luan Shu growth; Because the stock ratio is easier to obtain, and a bit of branch is only used in scion, takes this stock grafting method; The survival rate of seedling propagation is up to more than 85%, and breeding period is short, and reproduction coefficient is high; Can emerge in a large number, the quick breeding of Luan Shu being viewed and admired nursery stock has important promotional value.;
Embodiment
Embodiment 1:
Winter to early spring, in tree crown, 1 year livings diameter enriching of top clip growth, sleeping bud full branch healthy and strong at 1~2.5cm do scion, and branch is cut into 15~25cm, cloth wrapped up with wetting on robust growth, the gold branch Luan Shu elite stand that do not sprout as yet; Place the bulk container that is filled with water to heat in the container of splendid attire paraffin; When treating water temperature rise to the 100 ℃ left and right sides; Taking-up immediately in the paraffin that melts is immersed in one scion; Turn around again and dip in the other end, make the appearance of whole scion all cover the very thin paraffin of one deck, be placed on the low temperature place refrigeration about 5 ℃ then; Grafting when March, the left and right sides on the 15th Luan Shu nursery stock did not sprout as yet in spring; The branch of preserving is cut into the long section of 5cm does scion; From apart from the nearest back side, bud position of lower cut; With grafting knife downward parallel end that is cut to the scion axis when inscribe reaches xylem, the long 3cm of tangent plane splays into the 0.5cm inclined-plane with scion bevel offside subsequently.Scion cut thickness be 0.3cm; Select 1 year living Luan Shu seedling of the leading thread 2cm of robust growth, no damage by disease and insect to make stock; 3cm cuts off at the place apart from ground with stock, scabbles section, is used for the then downward vertical cut at the edge of xylem of cut-grafting, impose uniformity without examining individual cases, and kerf width and scion equal diameters reach 2cm deeply; Promptly inwards, insert the stock otch, and be inserted into the end, make scion aim at snap with the cambium of stock the big bevel of scion.If anvil, fringe do not wait slightly, can aim at cambium on one side, use the bottom-up whipping of plastic tape tight then; Be carried out to biopsy after the grafting in 10~15 days, the bud in the scion keeps fresh or germination and growth then shows and become alive.In time wipe out branch and the root sprout tillers branch sprouted on the grafting stock, guarantee the nutrition supply and the growth of scion.The scion sprout growth is removed the bondage of plastic tape after 1~2 month.Should keep the moistening of field soil between the grafting healing stage, grafting can carry out management such as normal field weeding, fertilising after becoming to live, and the method graft survival rate can reach 86.8%.
Embodiment 2:
Winter to early spring, in tree crown, 1 year livings diameter enriching of top clip growth, sleeping bud full branch healthy and strong at 1~2.5cm do scion, and branch is cut into 15~25cm, cloth wrapped up with wetting on robust growth, the gold branch Luan Shu elite stand that do not sprout as yet; Place the bulk container that is filled with water to heat in the container of splendid attire paraffin; When treating water temperature rise to the 100 ℃ left and right sides; Taking-up immediately in the paraffin that melts is immersed in one scion; Turn around again and dip in the other end, make the appearance of whole scion all cover the very thin paraffin of one deck, be placed on the low temperature place refrigeration about 5 ℃ then; Grafting when March, the left and right sides on the 15th Luan Shu nursery stock did not sprout as yet in spring; The branch of preserving is cut into the long section of 8cm does scion; From apart from the nearest back side, bud position of lower cut; With grafting knife downward parallel end that is cut to the scion axis when inscribe reaches xylem, the long 5cm of tangent plane splays into the 1cm inclined-plane with scion bevel offside subsequently.Scion cut thickness be 0.8cm; Select 2 years living Luan Shu seedlings of the leading thread 4cm of robust growth, no damage by disease and insect to make stock; 4cm cuts off at the place apart from ground with stock, scabbles section, is used for the then downward vertical cut at the edge of xylem of cut-grafting, impose uniformity without examining individual cases, and kerf width and scion equal diameters reach 3cm deeply; Promptly inwards, insert the stock otch, and be inserted into the end, make scion aim at snap with the cambium of stock the big bevel of scion.If anvil, fringe do not wait slightly, can aim at cambium on one side, use the bottom-up whipping of plastic tape tight then; Be carried out to biopsy after the grafting in 10~15 days, the bud in the scion keeps fresh or germination and growth then shows and become alive.In time wipe out branch and the root sprout tillers branch sprouted on the grafting stock, guarantee the nutrition supply and the growth of scion.The scion sprout growth is removed the bondage of plastic tape after 1~2 month.Should keep the moistening of field soil between the grafting healing stage, grafting can carry out management such as normal field weeding, fertilising after becoming to live, and the method graft survival rate can reach 95.6%.
Embodiment 3:
Winter to early spring, in tree crown, 1 year livings diameter enriching of top clip growth, sleeping bud full branch healthy and strong at 1~2.5cm do scion, and branch is cut into 15~25cm, cloth wrapped up with wetting on robust growth, the gold branch Luan Shu elite stand that do not sprout as yet; Place the bulk container that is filled with water to heat in the container of splendid attire paraffin; When treating water temperature rise to the 100 ℃ left and right sides; Taking-up immediately in the paraffin that melts is immersed in one scion; Turn around again and dip in the other end, make the appearance of whole scion all cover the very thin paraffin of one deck, be placed on the low temperature place refrigeration about 5 ℃ then; Grafting when March 25, the Luan Shu nursery stock was about to sprout in spring; The branch of preserving is cut into the long section of 5cm does scion; From apart from the nearest back side, bud position of lower cut; With grafting knife downward parallel end that is cut to the scion axis when inscribe reaches xylem, the long 3cm of tangent plane splays into the 0.5cm inclined-plane with scion bevel offside subsequently.Scion cut thickness generally at 0.3cm; Select robust growth, no damage by disease and insect 1 year living Luan Shu seedling leading thread 2cm make stock; 3cm cuts off at the place apart from ground with stock; Scabble section, the stock cortex is vertically cut an osculum, length is 1/2 of scion bevel length; Insert facing to xylem the long bevel of scion from the stock otch along xylem and in the middle of the phloem; And making the scion back side aim at stock otch center, white 0.5cm is stayed on bevel top during insertion, whips closely with plastic tape again; Be carried out to biopsy after the grafting in 10~15 days, the bud in the scion keeps fresh or germination and growth then shows and become alive.In time wipe out branch and the root sprout tillers branch sprouted on the grafting stock, guarantee the nutrition supply and the growth of scion.The scion sprout growth is removed the bondage of plastic tape after 1~2 month, simultaneously to becoming grafting alive to carry out helping, in case windbreak etc.Should keep the moistening of field soil between the grafting healing stage, grafting can carry out management such as normal field weeding, fertilising after becoming to live.The method graft survival rate can reach 82.5%.
Embodiment 4:
Winter to early spring, in tree crown, 1 year livings diameter enriching of top clip growth, sleeping bud full branch healthy and strong at 1~2.5cm do scion, and branch is cut into 15~25cm, cloth wrapped up with wetting on robust growth, the gold branch Luan Shu elite stand that do not sprout as yet; Place the bulk container that is filled with water to heat in the container of splendid attire paraffin; When treating water temperature rise to the 100 ℃ left and right sides; Taking-up immediately in the paraffin that melts is immersed in one scion; Turn around again and dip in the other end, make the appearance of whole scion all cover the very thin paraffin of one deck, be placed on the low temperature place refrigeration about 5 ℃ then; Grafting when March 25, the Luan Shu nursery stock was about to sprout in spring; The branch of preserving is cut into the long section of 5cm does scion; From apart from the nearest back side, bud position of lower cut; With grafting knife downward parallel end that is cut to the scion axis when inscribe reaches xylem, the long 5cm of tangent plane splays into the 1.0cm inclined-plane with scion bevel offside subsequently.Scion cut thickness generally at 0.5cm; Select the seedling of 2 years living Luan Shu leading thread 4cm of robust growth, no damage by disease and insect to make stock; 4cm cuts off at the place apart from ground with stock; Scabble section, the stock cortex is vertically cut an osculum, length is 2/3 of scion bevel length; Insert facing to xylem the long bevel of scion from the stock otch along xylem and in the middle of the phloem; And making the scion back side aim at stock otch center, white 1.0cm is stayed on bevel top during insertion, whips closely with plastic tape again; Be carried out to biopsy after the grafting in 10~15 days, the bud in the scion keeps fresh or germination and growth then shows and become alive.In time wipe out branch and the root sprout tillers branch sprouted on the grafting stock, guarantee the nutrition supply and the growth of scion.The scion sprout growth is removed the bondage of plastic tape after 1~2 month, simultaneously to becoming grafting alive to carry out helping, in case windbreak etc.Should keep the moistening of field soil between the grafting healing stage, grafting can carry out management such as normal field weeding, fertilising after becoming to live.The method graft survival rate can reach 91.3%.
The above is a preferred embodiments of the present invention.Be noted that for those skilled in the art under the prerequisite that does not break away from the principle of the invention, can also make some replacement or equivalent transformations of being equal to, these changes or replacement also should be regarded as protection scope of the present invention.

Claims (5)

1. gold branch Luan Shu grafting seedlings-growing method is characterized in that it is made up of the following step:
(1) choosing of scion: winter to early spring is the clip branch on robust growth, the gold branch Luan Shu elite stand that do not sprout as yet, and branch is cut into 15~25cm, wraps up with wet cloth, and is subsequent use;
(2) storage of scion: place the bulk container that is filled with water to heat in the container of splendid attire paraffin; When treating water temperature rise to the 100 ℃ left and right sides; One in the branch of step (1) is immersed taking-up immediately in the paraffin that melts; Turn around again and dip in the other end, make the appearance of whole scion all cover the very thin paraffin of one deck, be placed on the low temperature place refrigeration about 5 ℃ then.Subsequent use;
(3) making of scion: the branch after will preserve spring is cut into the long scion of 5~8cm, and the scion bottom is whittled into wedge shape, and according to the rugosity of stock, when stock was thick, scion can be cut thickly slightly, and when stock was thin, scion can be cut thin.Subsequent use;
(4) selection of stock with cut anvil: 3~4cm cuts off at the place Luan Shu stock of will not sprout apart from ground, scabbles section, and the downward vertical cut at the edge of xylem will impose uniformity without examining individual cases, and kerf width and scion equal diameters reach 2~3cm deeply; Begun to sprout like stock, 3~4cm cuts off at the place apart from ground with stock, scabbles section and gets final product, and is for use;
(5) engage or insert: the stock of not sprouting can be used cut-grafting, step (3) scion inserting step (4) stock otch, uses the bottom-up whipping of plastic tape tight then; For the stock that begins to sprout, the stock cortex is vertically cut an osculum, scion is inserted from the stock otch along xylem and in the middle of the phloem, whips closely with plastic tape again.
(6) management of grafting: be carried out to biopsy after the grafting in 10~15 days, the bud in the scion keeps fresh or germination and growth then shows and become alive, and survival rate can reach 80~90%.In time wipe out branch and the root sprout tillers branch sprouted on the grafting stock, guarantee the nutrition supply and the growth of scion.The scion sprout growth is removed the bondage of plastic tape after 1~2 month.Should keep the moistening of field soil between the grafting healing stage, grafting can carry out management such as normal field weeding, fertilising after becoming to live.
2. the described a kind of gold branch Luan Shu grafting method of claim 1, it is characterized in that: 1 year livings diameter that the said scion of step (1) is taked in tree crown, clip growth in top is enriched is at 1~2.5cm stalwartness, the full branch of sleeping bud;
3. the described a kind of gold branch Luan Shu grafting method of claim 1 is characterized in that: the time of the said grafting of step (3) 15 days~March 25 March in spring the Luan Shu nursery stock do not sprout as yet or carry out when being about to sprout; The cutting of scion get be with the scion bottom from apart from the nearest back side, bud position of lower cut, with grafting knife parallel end that is cut to the scion axis downwards when inscribe reaches xylem, tangent plane is grown 3~5cm, subsequently scion bevel offside is splayed into 0.5~1.0cm inclined-plane.Scion cut thickness generally at 0.3~0.5cm;
4. the described a kind of gold branch Luan Shu grafting method of claim 1 is characterized in that: 1~2 year living Luan Shu seedling of the leading thread 2~4cm of the said stock selection of step (4) robust growth, no damage by disease and insect;
5. the described a kind of gold branch Luan Shu grafting method of claim 1, it is characterized in that: the mode of the said grafting of step (5) is inserted the stock otch, and is inserted into the end for the big bevel of scion inwards, makes scion aim at snap with the cambium of stock.If anvil, fringe do not wait slightly, can aim at cambium on one side; For beginning the stock of leading thread more than 2cm of sprouting; The stock cortex is vertically cut an osculum; Length is 1/2~2/3 of scion length, inserts facing to xylem the long bevel of scion from the stock otch along xylem and in the middle of the phloem, and makes the scion back side aim at stock otch center; White 0.5~1.0cm is stayed on bevel top during insertion, is beneficial to callus growth and wound healing.
CN201110226203A 2011-08-09 2011-08-09 Method for raising seedlings of golden bough goldenrain tree by grafting Pending CN102301923A (en)

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CN103004477A (en) * 2012-09-28 2013-04-03 北京林业大学 Fast-growth cultivation method of top-grafting gold-leaf goldenrain tree
CN103141312A (en) * 2013-04-03 2013-06-12 南京林业大学 Grafting and seedling raising method for European hornbeam
CN104025848A (en) * 2014-05-26 2014-09-10 安徽恩龙园林股份有限公司 Koelreuteria bipinnata franchet cultivation method
CN104126571A (en) * 2014-07-18 2014-11-05 山东农业大学 Ginkgo biloba scion fresh-keeping method
CN104285682A (en) * 2014-08-22 2015-01-21 北京林业大学 Koelrenteria Paniculata var. grafting method
CN104737904A (en) * 2014-10-16 2015-07-01 江苏省林业科学研究院 Culture medium for tissue culture of Koelreuteria bipinnata var. integrifoliola 'Jinyan'
CN105724256A (en) * 2015-05-07 2016-07-06 江苏省林业科学研究院 Mini-type grafting method of Koelreuteria bipinnata var. integrifoliola 'Jinyan'
CN106234110A (en) * 2016-07-29 2016-12-21 广西壮族自治区林业科学研究院 A kind of Phoebe bournei grafting seedlings-growing method
CN106305280A (en) * 2016-08-19 2017-01-11 江苏艺轩园林景观工程有限公司 Cutting propagation method for koelreuteria paniculata
CN106900355A (en) * 2017-02-18 2017-06-30 天长市圣丰生态农业科技有限公司 One seed pod Pu is big, seed big, the method for the Kiwi berry seedling grafting of dimensionally stable
CN107371828A (en) * 2017-09-05 2017-11-24 北京中农富通园艺有限公司 A kind of method with overlength scion grafting renovation fruit tree
CN110178563A (en) * 2019-06-25 2019-08-30 四川七彩林科股份有限公司 Acer palmatum cut-grafting cultural method
CN110278787A (en) * 2019-06-25 2019-09-27 四川七彩林科股份有限公司 Fringe bar pretreating process in Acer palmatum cut-grafting cultivation
CN111034485A (en) * 2019-12-14 2020-04-21 山西搏宇农林开发有限公司 Artificial Chinese honeylocust grafting method
CN111567246A (en) * 2020-05-20 2020-08-25 北京都润生态环境工程有限公司 Method for grafting autumn purple Chinese ash in garden seedling breeding
CN111937611A (en) * 2020-08-24 2020-11-17 牛红霞 Koelreuteria paniculata grafting method

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Cited By (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103004477A (en) * 2012-09-28 2013-04-03 北京林业大学 Fast-growth cultivation method of top-grafting gold-leaf goldenrain tree
CN103004477B (en) * 2012-09-28 2014-02-19 北京林业大学 Fast-growth cultivation method of top-grafting gold-leaf goldenrain tree
CN103141312A (en) * 2013-04-03 2013-06-12 南京林业大学 Grafting and seedling raising method for European hornbeam
CN104025848A (en) * 2014-05-26 2014-09-10 安徽恩龙园林股份有限公司 Koelreuteria bipinnata franchet cultivation method
CN104126571A (en) * 2014-07-18 2014-11-05 山东农业大学 Ginkgo biloba scion fresh-keeping method
CN104285682A (en) * 2014-08-22 2015-01-21 北京林业大学 Koelrenteria Paniculata var. grafting method
CN104737904A (en) * 2014-10-16 2015-07-01 江苏省林业科学研究院 Culture medium for tissue culture of Koelreuteria bipinnata var. integrifoliola 'Jinyan'
CN105724256A (en) * 2015-05-07 2016-07-06 江苏省林业科学研究院 Mini-type grafting method of Koelreuteria bipinnata var. integrifoliola 'Jinyan'
CN106234110A (en) * 2016-07-29 2016-12-21 广西壮族自治区林业科学研究院 A kind of Phoebe bournei grafting seedlings-growing method
CN106305280A (en) * 2016-08-19 2017-01-11 江苏艺轩园林景观工程有限公司 Cutting propagation method for koelreuteria paniculata
CN106900355A (en) * 2017-02-18 2017-06-30 天长市圣丰生态农业科技有限公司 One seed pod Pu is big, seed big, the method for the Kiwi berry seedling grafting of dimensionally stable
CN107371828A (en) * 2017-09-05 2017-11-24 北京中农富通园艺有限公司 A kind of method with overlength scion grafting renovation fruit tree
CN110178563A (en) * 2019-06-25 2019-08-30 四川七彩林科股份有限公司 Acer palmatum cut-grafting cultural method
CN110278787A (en) * 2019-06-25 2019-09-27 四川七彩林科股份有限公司 Fringe bar pretreating process in Acer palmatum cut-grafting cultivation
CN111034485A (en) * 2019-12-14 2020-04-21 山西搏宇农林开发有限公司 Artificial Chinese honeylocust grafting method
CN111567246A (en) * 2020-05-20 2020-08-25 北京都润生态环境工程有限公司 Method for grafting autumn purple Chinese ash in garden seedling breeding
CN111937611A (en) * 2020-08-24 2020-11-17 牛红霞 Koelreuteria paniculata grafting method

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Application publication date: 20120104