CN102188332B - Cosmetic containing oceanic shellfish active peptide and preparation method and application thereof - Google Patents
Cosmetic containing oceanic shellfish active peptide and preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses a cosmetic containing oceanic shellfish active peptide and a preparation method and application thereof. The cosmetic contains 0.1 to 10 percent of oceanic shellfish active peptide, or 0.1 to 10 percent of oceanic shellfish active peptide and 1 to 10 percent of hyaluronic acid, or 0.1 to 10 percent of oceanic shellfish active peptide, 1 to 10 percent of hyaluronic acid and 1 to 25 percent of vitamin C (VC), and the balance is cosmetic matrix components. The peptide is formed by boiling, ultrasonic, enzymolysis and ultra-filtration of oceanic shellfish meat; and besides the active peptide, the oceanic shellfish meat also contains free amino acid, glycosaminoglycan, polyunsaturated fatty acid, vitamins, trace elements and the like. The problems of application and the like of the oceanic shellfish active peptide in the cosmetic are solved; and the prepared cream, emulsion, aqueous agent, gel, aerosol or mask has more complete and remarkable skin-care or hair-care effect. After the hyaluronic acid and the VC are added, the effects of preserving moisture, increasing the elasticity and the smoothness of the skin, removing flaws and improving the skin color are remarkably enhanced.
Description
Technical field
The present invention relates to cosmetic field, be specifically related to a kind of cosmetics that contain oceanic shellfish active peptide and its preparation method and application.
Background technology
Since each large international cosmetic brand releases one after another " ocean series " cosmetics, contain the cosmetics of natural, safe marine bioactivity peptide components with its good moisturizing and skin moistening effect, cause rapidly the concern on beauty treatment circle and the favor of consumer, become the new focus of beauty treatment consumption.Yet the marine bioactivity peptide kind of adding in these cosmetics is very limited, and majority is the marine collagen protein peptide, and other marine bioactivity peptide application in cosmetics is also very rare.
Owing to contain micro elements needed by human and the mineral such as abundant high quality protein, bioactive peptide, aminoacid, polyunsaturated fatty acid, polysaccharide and selenium, zinc, calcium, phosphorus, manganese, so that seashells has high nutrition and medical value.Wherein, oceanic shellfish active peptide has especially the immunity of adjusting, suppresses the multiple important function such as tumor, slow down aging, resisting fatigue, radioprotective, be widely used in food and the medicine, but its application in cosmetics is also few.
At present, existing patent of invention discloses the preparation method of seashells anti-oxidation peptide, and points out that it can be used for cosmetics, but unactual technique for applying technology and prescription do not relate to its true skin effect in cosmetics series yet.Simultaneously, because anti-oxidation peptide relative molecular weight or molecular weight ranges are defined, its effect only has antioxidant activity, also need with other active component work in coordination with composite, the beauty and skin care effect that just can realize ideal.And in the oceanic shellfish active peptide extraction process, be taken as the composition that impurity throws away and also contain abundant free amino acid, glycosaminoglycans, polyunsaturated fatty acid, vitamin and trace element, they are except having significant antioxidant activity, also have the restraint of tyrosinase activity, reduce UV-induced skin injury, check melanin generates, promote skin metabolism and the effects such as wound healing, promotion hair growth, and the antioxidant activity of peptide is also had significant potentiation.Yet these compositions but together are not applied in the cosmetics.
Summary of the invention
The object of the invention is to the problems such as rare for the contained marine bioactivity peptide kind that exists in the existing cosmetics, that the bioactive peptide effect is single or not obvious, the composition that has the beauty and skin care effect in the seashells is not fully utilized, be engaged in for a long time in the extraction and biological effectiveness research of marine bioactivity material through the inventor, what provide that a kind of preparation technology is easy, active component enriches, effect is remarkable, numerous in variety contains the oceanic shellfish active peptide cosmetics.
Another purpose of the present invention is to simplify the oceanic shellfish active peptide extraction process, adopt the ultrafilter membrane of 5000Da to carry out ultrafiltration, extract the mixture of bioactive peptide and free amino acid, glycosaminoglycans, polyunsaturated fatty acid, vitamin, trace element, and be made into cosmetics as raw material.Simplify active peptide extraction, cost-effective while, because its beauty and skin care effect is more comprehensive and remarkable, thereby also simplifying the process composite with other active component stabilisation.
A further object of the invention provides the preparation method and application of above-mentioned cosmetics, and by oceanic shellfish active peptide and hyaluronic acid and V
CCollaborative composite, make its moisturizing, increase skin elasticity, dispel wrinkle, dispel the flaws such as erythema/pimple, improve the effects such as the colour of skin and been significantly enhanced, thereby develop skin protection, the cosmetic hair care that is applicable to different skin quality.
Above-mentioned purpose is achieved by the following technical programs:
With ocean shellfish meat carry out homogenate, boil, enzyme-added rear ultrasonic pretreatment, enzymolysis, ultrafiltration, lyophilization, make pulverous molecular weight less than the mixture (being designated hereinafter simply as oceanic shellfish active peptide) of the bioactive peptide of 5000Da and free amino acid, glycosaminoglycans, polyunsaturated fatty acid, vitamin, trace element etc.It directly is configured to or with hyaluronic acid, V
CAfter collaborative composite, make cream frost, emulsion, water preparation, gel, aerosol or facial film, be used for skin protection (face, lip, neck neck, trunk, extremity) or hair care.
By weight percentage, contain oceanic shellfish active peptide 0.1%-10% in the cosmetics of the present invention, perhaps contain oceanic shellfish active peptide 0.1%-10% and hyaluronic acid 1%-10%, perhaps contain oceanic shellfish active peptide 0.1%-10%, hyaluronic acid 1%-10% and V
C1%-25%, remainder are the cosmetic base compositions
Described oceanic shellfish active peptide preparation method is preferably following steps: get edible seashells and shell and add 2-3 times of volume water after dirty, homogenate 3-5min, boil 5-10min, add protease 1000-3000U/g after being cooled to room temperature, 170w ultrasonic Treatment 5-8min under the room temperature, pH is 6.5-7.5, temperature is 40-60 ℃, behind the enzymolysis 15-60min, boiling water bath enzyme denaturing 6-8min, the centrifugal 10-15min of 8000r/min, supernatant are that the ultrafilter membrane of 5000Da carries out ultrafiltration by molecular cut off, make the oceanic shellfish active peptide powder after the lyophilization.Wherein, described seashells derives from one or more the combination in the common seashells such as Paphia Undulata, Corbiculida, Margarita, scallop, Concha Ostreae.Described protease is preferably one or more the combination in bromelain, papain, neutral protease, alkaline protease, pepsin, trypsin, the subtilisin.
Main component to oceanic shellfish active peptide powder of the present invention is analyzed, and the results are shown in Table 1.
Table 1 oceanic shellfish active peptide powder main component is analyzed
Contain abundant free amino acid in the oceanic shellfish active peptide powder of the present invention, wherein serine, glycine, threonine and alanine are Moisture factor natural in the horny layer, methionine and cysteine are keratin important composition compositions, and glycine, proline and alanine are collagen protein important composition compositions.Arginine energy vasodilator promotes skin blood to flow and metabolism.Glutamatergic promotes cell proliferation and differentiation, promotes collagen protein to generate and wound healing.Ornithine can improve blood flow, promotes cell proliferation and skin regeneration.Methionine and histidine have antioxidant activity, and glutamic acid and cysteine not only have antioxidant activity, also have restraint of tyrosinase active, thereby can protect skin to avoid ultraviolet injury, and can generate by check melanin.
Also contain abundant glycosaminoglycans, polyunsaturated fatty acid, V in the oceanic shellfish active peptide powder of the present invention
A, V
B1, V
B2, V
B6, V
C, V
E, Zn and Se.The glycosaminoglycans moistening effect is good, and its antioxidant activity is highly significant also.Polyunsaturated fatty acid can make skin become delicate moist, and can promote hair growth, improves hair quality.V
ABut the chafe enzymatic activity improves skin natural cover for defense function and elasticity, and epidermis is thickened, and repairs UV-induced damage, delay skin aging.V
BBe amino acid metabolism and keep the requisite composition of skin health, have the effect of regulating fat secretion, promoting skin oil-water balance.V
CCan promote collagen protein synthesis, improve skin elasticity, with V
ECoupling can protect skin to avoid ultraviolet injury, but and bright color.V
EBe a kind of good antioxidant, can increase horny layer humidity, improve the skin smooth degree, eliminate scytitis, erythema and swelling, strengthen skin to the resistance of uv damage, protection skin is avoided radical damage.Zn is indispensable composition in the superoxide dismutase, can improve ulcer, reduce inflammation, and can promote wound healing, reduces pigmentation.Se is the active center of glutathion peroxidase, can resist UV-induced apoptosis, to acne and dermatitis to improve effect very obvious.
The distribution of oceanic shellfish active peptide relative molecular weight and antioxidant activity among the present invention are analyzed, be the results are shown in Table 2.
Table 2 oceanic shellfish active peptide relative molecular weight distributes and antioxidant activity (%)
Free radical scavenging activity is measured under 5mg/mL
In oceanic shellfish active peptide powder of the present invention, molecular weight is less than 50% of 500Da-5000Da bioactive peptide content.It is lower to limit molecular weight or molecular weight ranges bioactive peptide content, and its corresponding antioxidant activity does not increase and proportional raising with purity.This explanation is also being brought into play significant antioxidant activity at compositions such as oceanic shellfish active peptide powder Free Amino Acids, glycosaminoglycans.
Prepare in accordance with the following steps the cosmetics that contain oceanic shellfish active peptide among the present invention: oceanic shellfish active peptide directly is pre-configured to concentration as the 10%-50% aqueous solution take the form of powdery or with deionized water, makes cream frost, emulsion, water preparation, gel, aerosol or facial film with the cosmetic base homogenizing or after mixing again.Cosmetics exquisite quality among the present invention, glossy, good stability, coating is even, spreadability is good, and skin easily absorbs, can be irritated.
Choose 80 of healthy volunteers, man, woman half and half, age 20-60 year, without serious systemic disease, without immunodeficiency or autoimmune disease, without the activeness anaphylactic disease, to skin-care cosmetics without the severe allergy history, do not used hormone medicine or immunosuppressant in nearly 1 month, do not participated in other clinical trials, do not smear the external preparation such as any cosmetics.The cosmetics that only contain oceanic shellfish active peptide that the present invention is made, only contain oceanic shellfish active peptide and hyaluronic cosmetics, contain oceanic shellfish active peptide, hyaluronic acid and V
CCosmetics carry out moisturizing, skin effect evaluation.
Smear 1,2,4,6 and 8h after, use the hydration status of moisture of skin measuring instrument Corneometer CM825 test skin, the water loss amount of percutaneous moisture loss measuring instrument Tewameter TM300 test skin the results are shown in Table 3.After using for 8 weeks continuously, relatively to the improvement situation of skin, the results are shown in Table 4.
The moistening effect of table 3 pair skin
Table 4 uses rear improvement situation to skin of 8 weeks
The 500-1000Da seashells peptide cosmetics the highest with containing antioxidant activity are compared, the cosmetics that only contain oceanic shellfish active peptide among the present invention can significantly increase moisture of skin, and minimizing moisture loss, after using for 8 weeks, skin elasticity, wrinkle, degree of compacting, drying, pruritus, erythema/pimple and the colour of skin have all obtained obvious improvement, its beauty and skin care effect is more comprehensive, be applicable to that hydropenia, elasticity are not enough, wrinkle obviously, slightly there is the skin quality of the flaw such as erythema/pimple in face.
Hyaluronic cosmetics have been added among the present invention, it increases skin elasticity and degree of compacting, smooth wrinkle, improve the dry and dry effects such as skin pruritus that cause has all obtained remarkable enhancing, other beauty and skin care effect also increases, be applicable to that hydropenia is serious, skin pruritus, wrinkle obviously, the more skin quality of flaw such as erythema/pimple.
Hyaluronic acid and V have been added among the present invention
CCosmetics, except it increases skin elasticity and degree of compacting, smooths wrinkle, improves drying and skin pruritus effect be improved, it improves skin coarseness and the colour of skin, dispel the effects such as skin blemishes such as erythema/pimple also significantly strengthens, and is applicable to pachylosis, long erythema/pimple, the obscure skin quality that serious skin problem is arranged of the colour of skin is arranged.
Compared with prior art, the present invention has following beneficial effect:
The raw materials used relevant marine product Application standard of China that meets, source safety is in liberal supply.In extraction process, adopt homogenate, decocting in water, ultrasonic pretreatment, make the small-molecule substance rates of release such as aminoacid, polyunsaturated fatty acid, vitamin and trace element and gain in yield more than 2 times.Ultrasound wave improves enzymolysis efficiency by changing enzyme texture image, will foreshorten in the 1h in the response time.Ultrafiltration technology is simple, easy to operate, and the active substance loss is few, and yield is more than 90%.
Simplified the oceanic shellfish active peptide extraction process that is added in the cosmetics, except containing bioactive peptide, also contain the composition that free amino acid, glycosaminoglycans, polyunsaturated fatty acid, vitamin and trace element etc. have remarkable beauty and skin care effect in the Gly-His-Lys that extracts.Because its beauty and skin care effect is more comprehensive and remarkable, has also simplified itself and other active substance and worked in coordination with composite technique.
Utilize hyaluronic acid and V
CThe oceanic shellfish active peptide skin-care effect is had the effect of remarkable enhancing, in cosmetics, add hyaluronic acid and V
C, make its moisturizing, wrinkle removal, increase skin elasticity, dispel face's flaw, improve the effects such as the colour of skin and greatly improve.
The specific embodiment
Following examples are to further specify of the present invention, are not limitations of the present invention.
Embodiment 1:
Get the edible Paphia Undulata that meets the relevant marine product Application standard of China, add 2 times of volume pure water homogenate 4min behind the internal organs that shell, boil 5min, add papain after being cooled to room temperature, enzyme concentration is 1000U/g, and behind the 170w ultrasonic Treatment 8min, pH is 7 under the room temperature, temperature is 50 ℃ of enzymolysis 60min, boiling water bath enzyme denaturing 6min, the centrifugal 15min of 8000r/m, supernatant is the ultrafiltration of 5000Da ultrafilter membrane by molecular cut off, make the oceanic shellfish active peptide powder after the lyophilization, stand-by.
The preparation of oil phase thing: by the shared mass fraction of each raw material, stearic acid 2, lanoline 1, white oil 6, dimethicone 2, isopropyl palmitate 2.Choose stearic acid, lanoline, white oil, dimethicone and isopropyl palmitate and mix, under the condition that constantly stirs, be heated to 85-90 ℃, keep the 20min sterilization, get the oil phase thing, stand-by.
The aqueous-phase material preparation: by the shared mass fraction of each raw material, glycerol 2, oceanic shellfish active peptide 10, distilled water 68.Glycerol and marine bioactivity peptide are added in the distilled water, be heated with stirring to 90-95 ℃, keep the 20min sterilization, be chilled to 85-90 ℃ and get aqueous-phase material, stand-by.
Emulsifying agent adds: by the shared mass fraction of each raw material, and oil phase thing 15.9, aqueous-phase material 80, monoglyceride 2, sodium lauryl sulphate 2, antiseptic 0.1.Monoglyceride is dissolved in the oil phase thing gets substance A, with the sodium lauryl sulphate substance B that is soluble in the aqueous phase in the thing to get; Heating also mixes substance A and substance B, is 3000r/min with homogenizing agitator adjusting rotary speed, stir 8min after, be cooled to room temperature, add while stirring antiseptic, it is white to make the cream that contains 10% oceanic shellfish active peptide.
The cream frost of present embodiment configuration is glossy, exquisite quality, be evenly distributed, profit do not separate mutually.Be coated in face, lip, trunk or extremity, easily absorb, continue to use one month, xerosis cutis, cracking before using, water profit, smooth after using.
Embodiment 2:
Getting the edible Corbiculida shells and adds 2.5 times of volume pure water homogenate 5min behind the internal organs, boil 10min, be cooled to and add bromelain and millet straw bacillus protease (1:1) after the room temperature, enzyme concentration is 2000U/g, under the room temperature behind the 170w ultrasonic Treatment 6min, pH is 6.5, and temperature is 40 ℃ of enzymolysis 30min, boiling water bath enzyme denaturing 7min, the centrifugal 12min of 8000r/m, supernatant is the ultrafiltration of 5000Da ultrafilter membrane by molecular cut off, makes the oceanic shellfish active peptide powder after the lyophilization, and is stand-by.
The A phase: by the shared mass fraction of each raw material, cyclopentasiloxane 1, dicaprylyl carbonate 1.Choose cyclopentasiloxane and dicaprylyl carbonate and mix, under the condition that constantly stirs, be heated to 80-95 ℃, keep the 20min sterilization, get A phase thing, stand-by.
The B phase: by the shared mass fraction of each raw material, glycerol 1, oceanic shellfish active peptide 6, hyaluronic acid 3, distilled water 69, xanthan gum 1.In glycerol, marine bioactivity peptide, hyaluronic acid, the former adding distilled water of Xanthan gum, be heated to 90-95 ℃ under stirring, keep the 20min sterilization, be chilled to 85-90 ℃ and make the B phase, stand-by.
The C phase: by the shared mass fraction of each raw material, Mel 2, perfume 1, antiseptic 1.With mixing under Mel, perfume, the antiseptic room temperature, make the C phase, stand-by.
By the shared mass fraction of each raw material, A phase 10, B phase 88, C phase 2.In the time of 70 ℃, the A phase is mixed homogenizing 20min mutually with B, be cooled to 35 ℃ and add while stirring the C phase, make and contain 6.6% oceanic shellfish active peptide, 3.3% hyaluronic emulsion.
The emulsion feel of present embodiment configuration is fine and smooth, coating is even, profit does not separate, easily absorption.Be coated in face, near the eyes or the neck neck, continue to use one month, skin had microgroove before user used, and the microgroove stricture of vagina that shoals, expresses one's feelings disappears after using.
Embodiment 3:
Get edible scallop, Margarita and shell and add 3 times of volume pure water homogenate 3min behind the internal organs, boil 8min, add alkaline protease after being cooled to room temperature, enzyme concentration is 3000U/g, behind the 170w ultrasonic Treatment 5min, be 7.5 in pH under the room temperature, temperature is under 60 ℃ of conditions, enzymolysis 15min, boiling water bath enzyme denaturing 8min, the centrifugal 10min of 8000r/m, supernatant are that the ultrafilter membrane of 5000Da carries out ultrafiltration by molecular cut off, make the oceanic shellfish active peptide powder after the lyophilization, stand-by.
The preparation of aqueous-phase material: by the shared mass percent of each raw material, oceanic shellfish active peptide 0.125, hyaluronic acid 1.25, V
C15, glycerol 3, potassium hydroxide 0.3, ethylenediaminetetraacetic acid 0.2, deionized water 80.125.At room temperature with oceanic shellfish active peptide, hyaluronic acid, V
C, glycerol, potassium hydroxide, ethylenediaminetetraacetic acid is dissolved in the deionized water, stirs to get aqueous-phase material.
The preparation of alcohol phase: by the shared mass fraction of each raw material, spice 0.1, antiseptic 0.1, polyvinylpyrrolidone 0.5, ethanol 5.3.At room temperature essence, antiseptic, polyvinylpyrrolidone are dissolved in the ethanol, stir to get alcohol phase.
Biphase mixing: by the shared mass fraction of each raw material, aqueous-phase material 80, alcohol phase 20.Under quick manual stirs, slowly alcohol phase is added in the aqueous-phase material and fully mix, homogenizing stirs and makes its dissolving evenly.After still aging, its supernatant is for containing 0.1% oceanic shellfish active peptide, 1% hyaluronic acid, 12%V
CWater preparation.
The water preparation of present embodiment configuration is without precipitation, high, the easily absorption, non-stimulated of transparency.Spread upon face, lip or neck neck, continue to use one month, xerosis cutis decortication before user uses, cheilogramma and neck stricture of vagina obviously, have erythema, the colour of skin obscure, skin moisturizing, flexible after using, lip and neck neck place skin texture shoal, and erythema disappears, and facial skin is pale, bright.
Embodiment 4:
According to the method for preparing the oceanic shellfish active peptide powder in the embodiment 1, used protease is pepsin and trypsin 1:1), make the oyster active peptides powder, stand-by.
Take by weighing each component according to following percentage by weight, oceanic shellfish active peptide powder 5%, hyaluronate sodium 0.3%, allantoin 0.8%, glycyrrhizic acid dipotassium 0.3%, beta glucan 1.5%, propylene glycol 8%, sodium hydroxide 0.4%, antiseptic 0.2%, deionized water 83.05%, acrylic acid (ester) class/alkylol acrylate (class) cross linked polymer 0.45%.
Drop into deionized water, acrylic acid (ester) class/alkylol acrylate (class) cross linked polymer in vacuum emulsification pot, 50r/m stirs and heats up; Hyaluronate sodium, mixed with propylene glycol are uniformly dispersed in the rear input vacuum emulsification pot, are warming up to 80 ℃, stir 20min; When being cooled to 60 ℃, add allantoin and glycyrrhizic acid dipotassium; When temperature is reduced to 45 ℃, add beta glucan and marine bioactivity peptide; Be evacuated to-0.05MPa 1500r/m homogenizing 10min; Add antiseptic, stir 10min; To be that 10% sodium hydroxide solution sucks in the vacuum emulsification pot with the concentration of deionized water configuration, stir 10min; Get rid of vacuum, discharging makes the gel that contains 5% marine bioactivity peptide.
The gained gel is transparent in the present embodiment, tissue is fine and smooth, without caking, under-10 ℃ and 40 ℃ of conditions, without paste separation.Be coated in face or neck neck, continue used one month, cutis laxa, dry hydropenia, corse sweat pore before user uses, skin-tightening after using, high resilience, glossy, pore dwindle to some extent.
Embodiment 5:
According to the method for preparing the oceanic shellfish active peptide powder in the embodiment 2, used protease is papain and neutral protease (1:1), makes the Paphia Undulata Gly-His-Lys, and is stand-by.
By the shared weight percent meter of each raw material, oceanic shellfish active peptide powder 4%, hyaluronic acid 10%, V
C1%, concentration is 75% ethanol 9%, deionized water 74.9%, perfume 1%, antiseptic 0.1%.The oceanic shellfish active peptide powder is dissolved in the deionized water, makes its whole dissolvings, add hyaluronic acid, V under the room temperature
C, concentration is 75% ethanol, perfume, antiseptic, stirs, make and contain 4% marine bioactivity peptide, 10% hyaluronic acid, 1% V
CAerosol.
The aerosol good stability of present embodiment configuration, fine and smooth without the globule of precipitation, formation spraying.Be sprayed on face, trunk, hair and root of hair place, continue to use one month, user uses the normal bellding light of front face, face and back length to have comedo, forehead place hair comparatively rare, fuel-displaced minimizing after using, comedo reduce, erythema is thin out, skin becomes bright and clean, and the alopecia symptom also alleviates to some extent, new piliation is comparatively sturdy, flexible.
Embodiment 6:
According to the method for preparing oceanic shellfish active peptide in the embodiment 3, select bromelain, make the mussel Gly-His-Lys.
By the shared weight percent meter of each raw material, oceanic shellfish active peptide powder 10%, hyaluronic acid 5%, V
C25%, nanometer Margarita powder 10%, Semen Armeniacae Amarum powder 11%, Mel 5%, V
E2%, deionized water 31%, antiseptic 1%.The oceanic shellfish active peptide powder is dissolved in the deionized water, makes it all after the dissolving, add in advance nanometer Margarita powder and the Semen Armeniacae Amarum powder of mixing under the room temperature, 50r/m stirring and evenly mixing 5min adds hyaluronic acid, V again
C, Mel, V
EAnd antiseptic, stirring and evenly mixing 5min makes and contains 10% marine bioactivity peptide, 5% hyaluronic acid, 25% V
CFacial film.
The facial film of present embodiment configuration is direct-edible, mouthfeel is good, also can directly spread on face, replenishes nutrient and moisture to skin.Continue to use one month, and pachylosis, dry pruritus before user uses, relaxed, have erythema, the colour of skin inhomogeneous, skin smooth after using, moist, delicate, flexible, erythema is thin out, whitening skin, stops using rear symptom not rebound.
Embodiment 7:
After the Paphia Undulata active peptide powder of embodiment 1 preparation is configured to aqueous solution that concentration is 10%-50% with deionized water, join in the common shampoo of buying in the supermarket, make the shampoo that contains 0.1% oceanic shellfish active peptide behind the homogenizing 10min.
Shampoo to the present embodiment preparation carries out human body experiment on probation, detects its hair care effect.20 of trial volunteers, the men and women half and half, age 20-45 year, crown hypotrichosis, jewelry, expensive clothing and other valuables.Before the test, statistics alopecia number every day, and measure newborn hair diameter and elasticity, add up continuously 10 days.Bring into use shampoo the 1st, 2,3,5, after 7 weeks, adding up the alopecia number, and measuring diameter and the extent of elasticity of newborn hair.Test finds, the shampoo that has added oceanic shellfish active peptide in use is after 5 weeks, and the alopecia number obviously reduces, with use before compare, average energy reduces by 20/d.Newborn hair thickness changes not obvious, but elasticity significantly strengthens, and illustrate that the shampoo that has added oceanic shellfish active peptide has good Anti-hair loss, educates an effect.
Embodiment 8:
According to the preparation method of embodiment 2, configuration contains 0.1% oceanic shellfish active peptide and 10% hyaluronic water preparation.The water preparation of present embodiment configuration is without precipitation, high, the easily absorption, non-stimulated of transparency.Spread upon face or neck neck, continue to use one month, xerosis cutis pruritus before user uses, wrinkle are obvious, and skin moisturizing, pruritus disappear after using, and wrinkle shoals.
Embodiment 9:
According to the preparation method of embodiment 1, configuration contains 10% oceanic shellfish active peptide and 1% hyaluronic cream frost.The cream frost of present embodiment configuration is glossy, exquisite quality, be evenly distributed.Be coated in face, lip, trunk or extremity, continue use one month, use front pachylosis, pigmentation is arranged, skin smooth, plain color calmness disappear after using.
Claims (7)
1. cosmetics that contain oceanic shellfish active peptide is characterized in that its raw material by percentage, contains oceanic shellfish active peptide 0.1%-10%, and remainder is the cosmetic base composition;
Described oceanic shellfish active peptide preparation method is: get shellfish and shell and add 2-3 times of volume water after dirty, homogenate 3-5min, boil 5-10min, add protease 1000-3000U/g after being cooled to room temperature, 170W ultrasonic Treatment 5-8min under the room temperature, pH is 6.5-7.5, temperature is 40-60 ℃, behind the enzymolysis 15-60min, boiling water bath enzyme denaturing 6-8min, the centrifugal 10-15min of 8000r/min, supernatant are that the ultrafilter membrane of 5000Da carries out ultrafiltration by molecular cut off, make the oceanic shellfish active peptide powder after the lyophilization;
Described protease is one or more the combination in bromelain, papain, neutral protease, alkaline protease, pepsin, trypsin, the subtilisin;
Described seashells derives from one or more the combination in Paphia Undulata, Corbiculida, Margarita, scallop, the Concha Ostreae.
2. the cosmetics that contain as claimed in claim 1 oceanic shellfish active peptide is characterized in that its raw material by percentage, contain oceanic shellfish active peptide 0.1%-10% and hyaluronic acid 1%-10%, and remainder is the cosmetic base composition.
3. the cosmetics that contain as claimed in claim 2 oceanic shellfish active peptide is characterized in that its raw material by percentage, contains oceanic shellfish active peptide 0.1%-10%, hyaluronic acid 1%-10% and V
C1%-25%, remainder are the cosmetic base compositions.
4. claim 1, the 2 or 3 described preparation methoies that contain the oceanic shellfish active peptide cosmetics, it is characterized in that oceanic shellfish active peptide is to be pre-configured to concentration as the 10%-50% aqueous solution take the form of powdery or with deionized water, with the cosmetic base homogenizing or mix.
5. as containing the cosmetics of oceanic shellfish active peptide as described in the claim 1,2 or 3, it is characterized in that comprising bioactive peptide, free amino acid, glycosaminoglycans, polyunsaturated fatty acid, vitamin and trace element in the described oceanic shellfish active peptide powder.
6. as containing the cosmetics of oceanic shellfish active peptide as described in the claim 1,2 or 3, it is characterized in that described cosmetics are cream frost, emulsion, water preparation, gel, aerosol or facial film.
7. claim 1, the 2 or 3 described application that contain the cosmetics of oceanic shellfish active peptide is characterized in that described used for cosmetic in skin protection or hair care, and described skin protection position refers to face, lip, neck neck, trunk or extremity.
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| CN102366383B (en) * | 2011-09-26 | 2012-12-26 | 佛山科学技术学院 | Marine organism functional cosmetic for nourishing skin around eyes |
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