CN108324591B - A hair composition containing marine bioactive substances - Google Patents

Abstract

The invention discloses a hair composition containing marine biological active substances, which specifically contains D-glucosamine hydrochloride, marine shellfish peptides, fish protein peptides, algal polysaccharides and piroctone olamine, and can be further supplemented with olive oil and panthenol. The hair composition of the present invention can be used as a shampoo product having a specific anti-dandruff function, such as an anti-dandruff shampoo, and the stratum corneum structure and normal barrier function of the scalp can be restored after use. The hair composition has multiple effects of keeping moisture for a long time, balancing oil secretion, inhibiting bacteria, diminishing inflammation, removing dandruff, relieving itching, etc., and can also significantly improve hair quality, reduce bifurcation, relieve alopecia, and promote dandruff removal and hair nourishing.

Description

A hair composition containing marine bioactive substances

Technical Field

The invention belongs to the field of cosmetics, and particularly relates to a composition for hair, in particular to a composition which takes D-glucosamine hydrochloride, marine shellfish peptide, fish protein peptide, algal polysaccharide and piroctone olamine as active ingredients and has the functions of bacteriostasis, inflammation diminishing, dandruff removing, itching relieving, scalp stratum corneum barrier repairing and hair quality improving.

Background

According to incomplete statistics, more than half of the population at the late adolescence stage of the world is plagued by dandruff. Typical dandruff is flaky, pruritic dandruff with a sticky scalp surface, and is a white or gray scale appearing when the scalp is abnormally diseased. The scales have larger particles, and are easy to fall off to shoulders when combing or scratching, so that the appearance is not only influenced, but also the trouble is brought to the work, the life and the social contact of people.

Dandruff formation is influenced by various factors such as malassezia colonization, sebum secretion and individual susceptibility, a defective stratum corneum barrier is a key factor causing dandruff, and a fragile epidermal penetration barrier is more easily damaged due to frequent use of shampoo. The surfactants in the shampoo can reduce the activity of water-soluble natural moisturizing factors and cutinase, and the strong irritant surfactants can aggravate the fragile epidermal penetration barrier with reduced lipid level, so that the scalp can often have inflammatory reactions such as tight feeling, dryness, irritation, pruritus and the like after washing. Severe or chronic stratum corneum damage can cause rapid water loss, abnormal epidermal hyperplasia, hyperkeratosis, lipid loss and disordered arrangement of stratum corneum structure, and disorder of skin natural barrier function, thereby generating a great amount of dandruff.

At present, most of the commercially available anti-dandruff products have a single treatment way and cannot directly repair the damaged horny layer of the dandruff-excessive scalp. And a large amount of antidandruff agents such as salicylic acid, zinc pyrithione, climbazole and the like are used to achieve the aims of bacteriostasis and sterilization, and hair scales are easy to be damaged. After long-term use, the hair becomes dry and split, and severe allergic reaction can occur, so that dandruff occurs periodically or continuously, and even hair follicles of the scalp are damaged, and permanent alopecia is caused. Therefore, the existing products are not enough to meet the requirements in the aspects of bacteriostasis, inflammation diminishing, dandruff removing and itching relieving. The current shampoo field is lack of effective products and researches in improving the barrier function of the scalp stratum corneum.

The marine biological active substance is a functional product prepared by green technologies such as enzymolysis and the like of marine organisms, contains rich active ingredients, and is proved by researches to be beneficial to moisturizing, diminishing inflammation and improving barrier function of skin. For example, patent ZL201510777169.2 discloses that D-glucosamine hydrochloride can be used in facial masks, which have various degrees of inhibitory effects on bacteria, yeasts, fungi, etc.; the patent ZL201110079404.0 uses marine shellfish peptide in cosmetics, and has effects of promoting collagen generation and wound healing, and improving skin natural barrier function; patent ZL201410294532.0 uses marine shellfish peptide in hair care product to reduce hair loss; patent ZL201110288716.2 uses algal polysaccharides in cosmetics, and can form a film on the skin surface, and has good moisturizing and repairing effects.

Disclosure of Invention

One object of the present invention is to provide a hair care composition.

It is another object of the present invention to provide a composition effective in improving the stratum corneum structure and normal barrier function of the scalp.

Another object of the present invention is to provide a composition having effects of maintaining moisture and balancing secretion of fats and oils for a long time.

The invention also aims to provide a composition with the effects of inhibiting bacteria, diminishing inflammation, removing dandruff and relieving itching.

It is another object of the present invention to provide a composition for improving hair quality, reducing split ends, and alleviating hair loss.

The invention realizes the purpose through the following technical scheme:

in one aspect, the present invention provides a composition comprising D-glucosamine hydrochloride for use in protecting hair or in the manufacture of a product for protecting hair.

As a preferred embodiment, the composition is used for preparing a product for protecting hair, and the content of D-glucosamine hydrochloride in the product is 0.01-0.1% by mass. As a more preferred embodiment, the content of D-glucosamine hydrochloride in the product is 0.01-0.05% by mass.

D-glucosamine hydrochloride is known to have good curative effects on treating rheumatic arthritis and gastric ulcer, has the effects of moisturizing, bacteriostasis and inflammation diminishing, and can be used for facial skin care. But research on their hair care efficacy and products is currently less involved. Through research, the inventor discovers for the first time that the D-glucosamine hydrochloride has the effects of film forming and hair protection, can reduce the combination of a surfactant and hair protein, helps to establish an antibacterial barrier, and enables malassezia metabolites and other microbial toxins to be difficult to invade.

The D-glucosamine hydrochloride can be obtained by any known method. For example, obtained through commercial purchase. Or, it may be extracted or prepared by existing methods, for example, by extraction from natural chitin.

As an alternative, the D-glucosamine hydrochloride is prepared by hydrolyzing chitosan with hydrochloric acid. For example, a certain amount of chitosan is weighed, diluted hydrochloric acid with the volume concentration of 3-5 times of 10% -15% is added, the temperature is 80-90 ℃, the reaction is carried out for 3-4 h, the filtration is carried out, activated carbon (the mass is 1% -2% of the volume of the solution) is added into the filtrate, the temperature is 65-70 ℃, the reaction is carried out for 2-3 h, the filtration is carried out, anhydrous ethanol with a certain volume is added into the filtrate until the final concentration of the ethanol is 65-85%, the mixture is kept stand for 8-12 h at the temperature of 0-8 ℃, and the D-glucosamine hydrochloride crystal is obtained by the filtration.

More specifically, chitosan is hydrolyzed with hydrochloric acid, for example. For example, a certain amount of chitosan is weighed, diluted hydrochloric acid with the volume concentration of 5 times of 15% is added, the temperature is 90 ℃, the reaction is carried out for 4 hours, the filtration is carried out, activated carbon (the mass is 2% of the volume of the solution) is added into the filtrate, the temperature is 70 ℃, the reaction is carried out for 2 hours, the filtration is carried out, a certain volume of absolute ethyl alcohol is added into the filtrate until the final concentration of the ethyl alcohol is 75%, the mixture is kept stand for 12 hours at the temperature of 4 ℃, and D-glucosamine hydrochloride crystals are obtained through.

Further, another aspect of the present invention provides a composition comprising marine bioactives, the composition comprising D-glucosamine hydrochloride, marine shellfish peptides, fish protein peptides, algal polysaccharides and piroctone olamine.

As a preferred embodiment, the composition of the present invention comprises the following components in parts by weight: 1-10 parts of D-glucosamine hydrochloride, 1-10 parts of marine shellfish peptide, 1-10 parts of fish protein peptide, 1-10 parts of algal polysaccharide and 10-100 parts of piroctone olamine; as a more preferred embodiment, the composition of the present invention comprises the following components in parts by weight: 1-5 parts of D-glucosamine hydrochloride, 1-5 parts of marine shellfish peptide, 1-5 parts of fish protein peptide, 1-5 parts of algal polysaccharide and 10-75 parts of piroctone olamine.

Wherein the preparation method and the efficacy of the D-glucosamine hydrochloride are as described above.

In the active ingredients, the marine shellfish peptide is a product obtained by carrying out protease enzymolysis on marine shellfish meat. The inventor uses the compound in skin care and hair care research to obtain good effect. However, due to the difference in physiological state and structure between the scalp when dandruff is generated and the skin of other parts of the human body, there is no report that a substance for general skin care can be used for the care of the scalp in a pathological state. The inventor firstly applies the marine shellfish peptide with the molecular weight range of 1KDa-3KDa to the nursing of the scalp under the scurf pathological state, and finds that the marine shellfish peptide can reduce the water loss on the surface of the scalp, stimulate hair follicles, improve microcirculation, improve the activities of SOD, CAT and GPx in skin tissues after being absorbed by the scalp, simultaneously can reduce the stimulation of an antidandruff agent to the scalp, relieve inflammatory reaction, repair the damaged skin structure and normal barrier function, improve the resistance of the scalp to bacteria and fungi, and further reduce the generation of the scurf.

These marine shellfish may include, but are not limited to, one or a combination of Paphia undulata, Pearl oyster, scallop, mussel, and oyster. The protease used may be an enzyme capable of hydrolyzing the protein, including but not limited to one or a combination of papain and alkaline protease.

As an alternative embodiment, the marine shellfish peptide is prepared by hydrolyzing shellfish meat with protease, and separating peptide with molecular weight less than 3 KDa; more preferably greater than 1KDa and less than 3 KDa; the method comprises the following specific steps: removing shell and viscera of marine shellfish, adding 3-5 times volume of purified water, homogenizing, adding protease (2000U/g shellfish meat-3000U/g shellfish meat), inactivating enzyme in boiling water bath at pH of 6.5-7.5, temperature of 40-50 deg.C, performing enzymolysis for 4-6 h, centrifuging for 8-12min, 8000r/m-10000r/m for 10-15 min, ultrafiltering supernatant with 3KDa ultrafiltration membrane, ultrafiltering filtrate with 1KDa ultrafiltration membrane, and spray drying the filtrate.

For example, more specifically, marine shellfish is subjected to shelling and viscera removal, then 4 times volume of purified water is added for homogenate, 3000U/g shellfish meat of protease is added, enzymolysis is carried out for 4h at the pH value of 7 and the temperature of 50 ℃, enzyme deactivation is carried out in boiling water bath for 10min, centrifugation is carried out for 15min at 8000r/m, supernatant is subjected to ultrafiltration by an ultrafiltration membrane with the cut-off molecular weight of 3KDa, filtrate is subjected to ultrafiltration by an ultrafiltration membrane with the cut-off molecular weight of 1KDa, and the cut-off solution is subjected to spray drying to obtain.

In the active ingredients, the fish protein peptide is a product obtained by carrying out enzymolysis on marine fish meat by protease, and can relieve skin tension, reduce stimulation of a surfactant to scalp and damage of protein and lipid membranes, and maintain stratum corneum integrity and water balance. These marine fish species may include, but are not limited to, one or a combination of tuna and salmon. The protease used may be an enzyme capable of enzymatically hydrolyzing a protein, including but not limited to alkaline protease.

As an embodiment, the fish protein peptide is prepared by hydrolyzing fish with protease, and separating peptide with molecular weight less than 10 KDa; the method comprises the following specific steps: adding purified water with volume of 3-5 times of that of marine fish meat, homogenizing, adding alkaline protease, adding enzyme at 3000U/g fish meat-4000U/g fish meat, inactivating enzyme in boiling water bath at pH of 6.5-7.5 and temperature of 40-55 deg.C for 4-6 h, centrifuging at 8000 ion 10000r/m for 10-15 min, ultrafiltering supernatant with ultrafiltration membrane with molecular weight cutoff of 10KDa, and spray-drying filtrate.

For example, more specifically, the preparation method comprises adding 3 times volume of purified water into marine fish meat, homogenizing, adding alkaline protease, adding 4000U/g of fish meat, performing enzymolysis at pH 7 and 50 deg.C for 5h, inactivating enzyme in boiling water bath for 10min, centrifuging at 8000r/m for 15min, ultrafiltering supernatant with ultrafiltration membrane with molecular weight cutoff of 10KDa, and spray-drying filtrate.

Algal polysaccharides are common raw materials in the fields of cosmetics, foods, medicines and the like. Algal polysaccharides are products obtained by enzymatic hydrolysis of algae with cellulase and pectinase. These seaweeds may include, but are not limited to, one or a combination of several of laver, sargassum fusiforme, kelp, spirulina, kelp, fucus, and the like. In the composition, the algal polysaccharides can reduce the water loss on the skin surface, balance the oil secretion (reduce the breeding of malassezia lipotropica due to the reduction of the nutrient source of the malassezia lipotropica), maintain the moisture content and the intrinsic enzyme activity of the horny layer, promote the penetration and absorption of nutrient substances, and maintain the integrity and the health of the scalp.

Algal polysaccharides are commercially available from sources or may be prepared by enzymatic hydrolysis.

For example, as an alternative embodiment, algal polysaccharides are prepared by hydrolyzing algae with cellulase and pectinase, and isolating an enzymatic hydrolysate having a molecular weight greater than 5 kDa; the method comprises the following specific steps: cleaning seaweed, mincing, adding 4-6 times volume of purified water, adding cellulase (3000U/g seaweed-4000U/g seaweed) and pectinase (3000U/g seaweed-4000U/g seaweed) at pH of 6.5-7.5 and temperature of 60-75 deg.C, performing enzymolysis for 3-5 h, inactivating enzyme in boiling water bath for 8-12min, and centrifuging at 8000r/m-10000r/m for 10-15 min. Ultrafiltering the supernatant with ultrafiltration membrane with cut-off molecular weight of 5KDa, concentrating the cut-off solution at 50-80 deg.C to original volume of 1/4, cooling, adding pre-cooled 0-8 deg.C anhydrous ethanol to final ethanol concentration of 65-85%, vacuum filtering, precipitating, and lyophilizing to obtain algal polysaccharide.

More specifically, for example, the seaweed is washed and minced, 5 times the volume of purified water is added, cellulase (4000U/g seaweed) and pectinase (3000U/g seaweed) are added at pH 7 and temperature of 70 ℃, the enzymolysis is carried out for 5h, the enzyme is inactivated in boiling water bath for 10min, and the centrifugation is carried out for 15min at 8000 r/m. Ultrafiltering the supernatant with ultrafiltration membrane with cut-off molecular weight of 5KDa, concentrating the cut-off solution at 80 deg.C to original volume of 1/4, cooling, adding pre-cooled 4 deg.C anhydrous ethanol to final ethanol concentration of 75%, vacuum filtering, precipitating, and lyophilizing to obtain algal polysaccharide.

Piroctone olamine is an anti-dandruff agent which can relieve scalp inflammation and reduce dandruff flaking, and is also a broad-spectrum antibacterial and bacteriostatic agent. The piroctone olamine salt is safe, non-toxic and non-irritant, can effectively kill yeasts generating dandruff, inhibits the growth of gram-positive and gram-negative fungi and moulds, has excellent solubility and compounding performance, cannot precipitate or layer after being mixed with cosmetic raw materials, and has the effects of thickening, corrosion prevention, deodorization and the like.

As a preferred embodiment, the composition of the present invention further comprises 10 to 100 parts by weight of olive oil and 10 to 100 parts by weight of panthenol in order to enhance the effects of repairing the barrier of the stratum corneum of the scalp and improving the hair quality. Preferably 10-50 parts by weight of olive oil, 10-50 parts by weight of panthenol.

The olive oil can permeate into stratum corneum, resist surfactant micelle to extract skin lipid, supplement endogenous fatty acid lost on the skin surface in the cleaning process, improve the level of structural lipid, repair the skin tissue structure and help to improve the skin barrier function. The panthenol is easy to be absorbed by the hair roots, enhances the hair follicle vitality, keeps moisture for a long time, promotes hair growth, reduces dandruff, is easy to be absorbed and attached to the hair, prevents the hair from being forked and damaged, increases the hair density, improves the hair quality, and enables the hair to be smooth and glossy.

In the above components, the D-glucosamine hydrochloride, marine shellfish peptide, fish protein peptide, algal polysaccharide, piroctone olamine, olive oil and panthenol can be prepared by the existing method or purchased from the market.

In a preferred embodiment of the invention, the composition is used for the preparation or use as a hair care product; preferably, for the preparation or use as a shampoo; is especially suitable for being used as shampoo with special function of removing dandruff. In addition, the composition of the invention can obviously improve the skin conditioning performance of the product; balance the secretion of scalp oil, such as obviously improving the glossiness of hair, making the hair smooth and easier to manage, effectively removing the tangle when the hair is washed, and effectively preventing the hair from frizzy, etc.

When the composition is used for preparing or being used as shampoo, the weight percentage of each component in the shampoo is 0.01-0.1 percent of D-glucosamine hydrochloride, 0.01-0.1 percent of marine shellfish peptide, 0.01-0.1 percent of fish protein peptide, 0.01-0.1 percent of algal polysaccharide and 0.1-1.0 percent of piroctone olamine.

As a preferred embodiment, the weight percentage of each component in the shampoo in the composition is as follows: 0.01-0.05% of D-glucosamine hydrochloride, 0.01-0.05% of marine shellfish peptide, 0.01-0.05% of fish protein peptide, 0.01-0.05% of algal polysaccharide and 0.1-0.75% of piroctone olamine salt.

Alternatively, as another preferred embodiment, when the composition is used for preparing or using as a shampoo, the weight percentage of each component in the shampoo is 0.01-0.1% of D-glucosamine hydrochloride, 0.01-0.1% of marine shellfish peptide, 0.01-0.1% of fish protein peptide, 0.01-0.1% of algal polysaccharide, 0.1-1.0% of piroctone olamine, 0.1-1.0% of olive oil and 0.1-1.0% of panthenol.

Or as another preferred embodiment, when the composition is used for preparing or using as shampoo, the weight percentage of each component in the shampoo is 0.01-0.05% of D-glucosamine hydrochloride, 0.01-0.05% of marine shellfish peptide, 0.01-0.05% of fish protein peptide, 0.01-0.05% of algal polysaccharide, 0.1-0.75% of piroctone olamine salt, 0.1-0.5% of olive oil and 0.1-0.5% of panthenol.

The above composition, used for preparing or as shampoo, can also comprise base component of shampoo such as surfactant, and the surfactant can provide cleaning effect and foaming property for the composition. The surfactant is not particularly limited, and may be a surfactant commonly used in shampoos in the prior art. Since the composition provided by the present invention is used as a functional ingredient, it is effective in reducing irritation of a surfactant and an antidandruff agent to the scalp, and in addition, has various special functions such as moisturizing, balancing oil secretion, and the like. Therefore, the surfactant type is selected broadly, and may be at least one selected from the group consisting of an anionic surfactant, a zwitterionic surfactant, and a nonionic surfactant.

Examples of the anionic surfactant group include, but are not limited to, sulfate, sulfonate, sarcosinate, and sarcosine derivatives. Preferred anionic surfactants are alkyl and alkyl ether sulfates. Alkyl sulfates such as ammonium lauryl sulfate, ammonium cocoyl sulfate, potassium lauryl sulfate, potassium cocoyl sulfate, sodium lauryl sulfate, sodium cocoyl sulfate, monoethanolamine lauryl sulfate, monoethanolamine cocoyl sulfate, diethanolamine lauryl sulfate, triethanolamine lauryl sulfate, triethylamine lauryl sulfate, and mixtures thereof; alkyl ether sulfates such as ammonium laureth sulfate, potassium laureth sulfate, sodium laureth sulfate, monoethanolamine laureth sulfate, diethanolamine laureth sulfate, triethanolamine laureth sulfate, triethylamine laureth sulfate, and mixtures thereof. Sulfonate anionic surfactants such as sodium tridecylbenzene sulfonate, sodium dodecylbenzene sulfonate, lauryl sulfosuccinate and the like. Sarcosine and sarcosine derivatives include, for example, sodium lauryl sarcosinate, cocoyl sarcosinate and mixtures thereof, with sodium lauryl sarcosinate being preferred. Other useful anionic surfactants are those derived from amino acids, including but not limited to N-acyl-L-glutamic acid, N-acyl sarcosine, salts thereof, and the like. In addition, suitable anionic surfactants may also include taurine derivatives, such as 2-aminoethanesulfonic acid, N-acyl-N-methyltaurine. Further suitable anionic surfactants are the products of fatty acids, for example fatty acids derived from coconut or palm oil, or fatty acid amides or potassium salts of methyl taurate, esterified with isethionic acid, neutralized with sodium hydroxide. Other suitable anionic surfactants are succinates such as disodium N-octadecyl sulfosuccinate, diamyl esters of sodium sulfosuccinate, dioctyl esters of sodium sulfosuccinate, and the like.

Among them, the nonionic surfactants are well known in the art, such as compounds having a hydrophobic group and a reactive hydrogen atom, for example, polyoxyethylene ethers of alkylphenols, fatty alcohol-polyoxyethylene ethers, spans, tweens, hydrogenated castor oils, and the like.

Wherein amphoteric surfactants are well known in the art, including, but not limited to, betaine zwitterionic compounds including alkyl betaines, sulfobetaines, amidoalkyl betaines, amidosulfobetaines, hydroxysulfobetaines, cocamidopropyl betaine, cocamidopropyl hydroxysulfobetaine, cocacyldimethylcarboxymethylbetaine, cocayldimethylsulfopropylbetaine, stearyldimethylsulfopropylbetaine, laurylamidobis- (2-hydroxyethyl) propyl sulfobetaine; or imidazoline type amphoteric surfactants such as 2-cocoyl-N-carboxymethyl-N-hydroxyethyl imidazolinebetaine, 2-lauryl-N-carboxymethyl-N-hydroxyethyl imidazolinebetaine, and the like.

When the composition is used in the preparation or as a shampoo, it may also include a cationic polymer to provide conditioning, particularly when the hair is wet, by assisting in the deposition of conditioning agents to the hair and scalp. The cationic polymer in the composition of the present invention may comprise the following: polymeric quaternary ammonium salts, cationic polysaccharides, vinyl monomer copolymers, vinyl pyrrolidone copolymers, and cationically modified proteins.

In addition to the above components, the composition of the present invention for preparing or for use as a shampoo may optionally comprise one or more of the following components: suspending agents, hair growth regulators, antistatic agents, foam boosters, viscosity modifiers and thickeners, pH adjusters, opacifiers or pearlescers, dyes, organic solvents or diluents, and the like. These components and their contents may be appropriately selected according to the actual requirements of the product, and added to the composition of the present invention.

All components used in the present invention should be dermatologically acceptable and not interfere with the performance of the original components of the invention in that they do not cause undue toxicity, incompatibility, instability, allergic response, and the like when contacted with human skin or otherwise compounded with other components.

In addition to the components described above, the compositions of the present invention may also contain an appropriate amount of water. The "proper amount of water" in the present invention means that the amount of water is adjusted according to actual needs. In the absence of other components, an "appropriate amount of water" may mean "the balance water".

The composition disclosed by the invention can effectively reduce the stimulation of a surfactant and an antidandruff agent to the scalp, keep the skin moist, balance the secretion of grease, effectively repair the stratum corneum structure and the normal barrier function of the scalp, and also has the effects of bacteriostasis, inflammation diminishing and the like. After the hair-care product is used, the phenomena of itching and scraps of scalp, greasy hair, bifurcation, falling off and the like are improved, and the hair becomes soft, smooth and full of luster.

The solution of the invention is based on the inventor's understanding of the key factors such as biophysics, biochemistry and ultrastructure change of scalp stratum corneum cell of dandruff patients, etiology and susceptibility mechanism influencing dandruff generation and development, and corresponding treatment method, and the efficacy of each functional active substance, and combining the research achievement of modern cosmetics and pharmacology, based on D-glucosamine hydrochloride, marine shellfish peptide, fish protein peptide, algal polysaccharide and piroctone olamine, olive oil and panthenol with synergistic effect are preferably selected from many active substances, scientific formula and synergistic compounding are carried out, and shampoo formula and process with remarkable dandruff removing effect are preferably selected.

The invention has the following outstanding advantages:

(1) aiming at the special physiological structure of the scalp stratum corneum of a dandruff patient, the anti-dandruff shampoo disclosed by the invention preferably selects pure natural marine shellfish peptides, fish protein peptides and safe, nontoxic and non-irritant anti-dandruff agent piroctone olamine, utilizes the film forming property of D-glucosamine hydrochloride and the moisturizing property of algal polysaccharide, promotes the transdermal absorption of nutrient substances and balances the secretion of grease while reducing the damage of the surfactant and the anti-dandruff agent to the hair protein and the scalp stratum corneum, and plays excellent anti-dandruff and itching relieving effects by establishing an antibacterial barrier and repairing the structure and function of the damaged stratum corneum.

(2) The composition of the invention plays roles in effectively reducing the stimulation of a surfactant and an anti-dandruff agent to the scalp, moisturizing water, balancing the secretion of grease, repairing the structure of the horny layer of the scalp and the normal barrier function, inhibiting bacteria, diminishing inflammation and the like, and has good hair quality improving effect while relieving itching and removing dandruff.

(3) The invention utilizes the stabilizing synergistic interaction among D-glucosamine hydrochloride, marine shellfish peptide, fish protein peptide, algal polysaccharide, piroctone olamine salt, olive oil and panthenol, so that the effects of balancing grease secretion, repairing cuticle barrier and improving hair quality are obviously improved.

(4) The composition of the invention is composed of natural components, is mild and non-irritating, and overcomes the problems of scalp itch, dandruff periodic recurrence, alopecia and the like caused by scalp and hair injury of the existing anti-dandruff shampoo.

Drawings

FIG. 1 is a graph of the zone of inhibition of sterile distilled water;

FIG. 2 is a piroctone olamine salt aqueous solution zone of inhibition assay;

FIG. 3 is a graph of the zone of inhibition assay for sample A1;

FIG. 4 is a zone of inhibition assay for sample A2;

FIG. 5 is a zone of inhibition assay for sample A3;

FIG. 6 is a zone of inhibition assay for sample B1;

FIG. 7 is a zone of inhibition assay for sample B2;

FIG. 8 is a plot of the zone of inhibition for sample B3.

Detailed Description

The invention is further illustrated but the embodiments of the invention are not limited by the following examples, and equivalent variations or modifications of the method according to the invention are to be considered within the scope of the invention. The starting materials used below are all commercially available, unless otherwise indicated.

Raw material information used in the examples:

the Chinese name is D-glucosamine hydrochloride

The English name is D-gluconamine Hydrochloride

CAS:66-84-2；

The Chinese name is piroctone olamine salt

English name of picoctone oligomer

CAS:68890-66-4。

In one embodiment of the invention, after the dandruff removing shampoo taking 0.01 to 0.1 percent of D-glucosamine hydrochloride, 0.01 to 0.1 percent of marine shellfish peptide, 0.01 to 0.1 percent of fish protein peptide, 0.01 to 0.1 percent of algal polysaccharide and 0.1 to 1.0 percent of piroctone olamine salt as active ingredients cleans the hair, compared with the reference substance without D-glucosamine hydrochloride, marine shellfish peptide, fish protein peptide, algal polysaccharide and piroctone olamine, the result shows that after the anti-dandruff shampoo containing D-glucosamine hydrochloride, marine shellfish peptide, fish protein peptide, algal polysaccharide and piroctone olamine is used, improved dandruff score by 2.83-3.13 times (about 17.4-19.0), improved itching score by 2.68-2.97 times (about 15.3-17.5), and improved Malassezia colony number by 5.58-7.77 times (25.2/cm).²26.5 pieces/cm²)。

In a preferred embodiment of the invention, the antidandruff shampoo is prepared by taking 0.01-0.1% of D-glucosamine hydrochloride, 0.01-0.1% of marine shellfish peptide, 0.01-0.1% of fish protein peptide, 0.01-0.1% of algal polysaccharide, 0.1-1.0% of piroctone olamine, 0.1-1.0% of olive oil and 0.1-1.0% of panthenol as main ingredients, and can be matched with other conventional shampoo matrixes to serve as an experimental sample; the control sample was a shampoo base without D-glucosamine hydrochloride, marine shellfish peptide, fish protein peptide, algal polysaccharide, piroctone olamine, olive oil, panthenol. The results show an improvement in dandruff score of 3 for the scalp treated with the test sample containing these ingredients compared to the control sample.73-3.96 times (about 23.4-24.9), improved pruritus score 3.59-3.83 times (about 21.5-20.2), and improved Malassezia colony number 5.03-7.88 times (25.0/cm)²-28.2 pieces/cm²)。

To further illustrate the protocol of the present invention, and to facilitate its use, the following examples are given by way of example to the preparation of anti-dandruff shampoos containing conventional cosmetic bases. It will be appreciated by those skilled in the art that the components contained in these examples are not intended as limitations on the practice of the invention, but are merely one alternative that may be practiced. The anti-dandruff shampoo can be prepared by adopting main active ingredients (D-glucosamine hydrochloride, marine shellfish peptide, fish protein peptide, algal polysaccharide, piroctone olamine, olive oil and panthenol) adopted by the invention and any multiple shampoo matrix components through a conventional process to be homogenized or mixed. For example, other materials than D-glucosamine hydrochloride, marine shellfish peptide, fish protein peptide, algal polysaccharide, piroctone olamine, olive oil and panthenol of examples 4-6, which are routinely substituted, would have similar beneficial effects when formulated into other types or the same type of shampoo products.

Example 1

Weighing chitosan meeting the use standard of relevant marine products in China, adding diluted hydrochloric acid with the volume concentration of 5 times being 15%, reacting for 4 hours at the temperature of 90 ℃, filtering, adding activated carbon (the mass is 2% of the volume of the solution), reacting for 2 hours at the temperature of 70 ℃, filtering, adding a certain volume of absolute ethyl alcohol into the filtrate until the final concentration of the ethyl alcohol is 75%, standing for 12 hours at the temperature of 4 ℃, and filtering to obtain D-glucosamine hydrochloride crystals.

Taking edible paphia undulata soft body part which meets the use standard of related marine products in China, removing shells and internal organs, adding 4 times of volume of purified water for homogenate, adding papain (3000U/g of shellfish meat), carrying out enzymolysis at the temperature of 50 ℃ for 4h, carrying out enzyme deactivation in boiling water bath for 10min, centrifuging at 8000r/m for 15min, carrying out ultrafiltration on supernatant through an ultrafiltration membrane with the cut-off molecular weight of 3KDa, carrying out ultrafiltration on filtrate through an ultrafiltration membrane with the cut-off molecular weight of 1KDa, and carrying out spray drying on the cut-off liquid to obtain the paphia undulata peptide.

Taking edible tuna meat meeting the use standard of related marine products in China, adding 3 times of purified water by volume, homogenizing, adding alkaline protease (4000U/g of fish meat), carrying out enzymolysis for 5h at the pH of 7 and the temperature of 50 ℃, inactivating enzyme in boiling water bath for 10min, centrifuging at 8000r/m for 15min, carrying out ultrafiltration on supernatant through an ultrafiltration membrane with the molecular weight cutoff of 10KDa, and carrying out spray drying on filtrate to obtain the tuna protein peptide.

Respectively weighing edible thallus Porphyrae and herba Zosterae Marinae meeting use standard of related marine products in China, cleaning, mincing, adding 5 times volume of purified water, adding cellulase (4000U/g Sargassum) and pectinase (3000U/g Sargassum) at pH of 7 and temperature of 70 deg.C, performing enzymolysis for 5 hr, inactivating enzyme in boiling water bath for 10min, and centrifuging at 8000r/m for 15 min. Ultrafiltering the supernatant with ultrafiltration membrane with cut-off molecular weight of 5KDa, concentrating the cut-off solution at 80 deg.C to original volume of 1/4, cooling, adding pre-cooled 4 deg.C anhydrous ethanol to final ethanol concentration of 75%, vacuum filtering, precipitating, and lyophilizing to obtain porphyran polysaccharide and laminarin, respectively.

The anti-dandruff shampoo comprises the following components in percentage by mass:

d-glucosamine hydrochloride 0.01%, Paphia undulata peptide 0.05%, tuna protein peptide 0.025%, porphyran polysaccharide 0.01%, laminarin 0.02%, piroctone olamine salt 0.5% purchased from Shanghai Hepu industries, Inc., myristic acid 4%, cocamide MEA 6%, ethylene glycol distearate 3%, sodium cocoamphoacetate 10%, sodium laureth-3 sulfate, decyl glucoside 5%, cocamidopropyl betaine 2.5%, polyquaternium-70.2%, DMDM hydantoin 0.4%, vanillin 1%, PEG-6 caprylic/capric glycerides 3%, glycerin 3%, and the balance of deionized water.

The anti-dandruff shampoo prepared by the embodiment has mild effect and abundant foam, can effectively clean hair and scalp, feels comfortable and relaxed after washing, and greatly relieves the symptoms of grease secretion and pruritus. After 2 weeks of continuous use, there was a significant reduction in dandruff.

Example 2

D-glucosamine hydrochloride was prepared according to the procedure of example 1.

Taking edible soft body parts of pearl oyster meeting the use standard of relevant marine products in China, removing shells and internal organs, adding 4 times of volume of purified water for homogenate, adding alkaline protease (3000U/g oyster meat), carrying out enzymolysis at 50 ℃ and pH 7 for 4h, carrying out enzyme deactivation in boiling water bath for 10min, centrifuging at 8000r/m for 15min, carrying out ultrafiltration on supernatant by using an ultrafiltration membrane with molecular weight cutoff of 3KDa, carrying out ultrafiltration on filtrate by using an ultrafiltration membrane with molecular weight cutoff of 1KDa, and carrying out spray drying on the retentate to obtain the pearl oyster peptide.

Salmon protein peptide was obtained by the method of example 1.

The sargassum fusiforme polysaccharide was prepared according to the method of example 1.

The anti-dandruff shampoo comprises the following components in percentage by mass: 0.05% of D-glucosamine hydrochloride, 0.01% of pearl oyster peptide, 0.01% of salmon protein peptide, 0.02% of sargassum fusiforme polysaccharide, 0.1% of piroctone olamine salt, 100.25% of polyquaternary ammonium salt, 1.5% of PEG-120 methyl glucose dioleate, 2.5% of disodium laureth sulfosuccinate (mass fraction of 40%), 7.7% of sodium cocoamphoacetate (mass fraction of 39%), 25% of sodium laureth-3 sulfate (mass fraction of 28%), 1% of ethylene glycol distearate/laureth-4/cocamidopropyl betaine, 1.5% of propylene glycol, 0.45% of sodium chloride, 0.03% of citric acid, 0.2% of imidazolidinyl urea, 0.5% of lauryl aldehyde and the balance of deionized water.

The anti-dandruff shampoo prepared by the embodiment has the effects of cleaning and conditioning, can effectively clean hair and scalp, and can remove dandruff, relieve itching and repair damaged scalp cuticle and hair scales. After 2 weeks of continuous use, the hair was no longer greasy, became soft, easy to comb, glossy, and the dandruff disappeared.

Example 3

Taking edible scallop soft body meeting the use standard of related marine products in China, removing shells and internal organs, adding 4 times of volume of purified water for homogenate, adding papain (3000U/g shellfish meat) and alkaline protease (3000U/g shellfish meat), carrying out enzymolysis for 4h at the pH of 7 and the temperature of 50 ℃, inactivating enzymes in boiling water bath for 10min, centrifuging at 8000r/m for 15min, carrying out ultrafiltration on supernatant through an ultrafiltration membrane with the molecular weight cutoff of 3KDa, carrying out ultrafiltration on filtrate through an ultrafiltration membrane with the molecular weight cutoff of 1KDa, and carrying out spray drying on the retentate to obtain the scallop peptide.

Tuna protein peptide and salmon protein peptide were prepared according to the method of example 1.

Spirulina polysaccharide was prepared according to the method of example 1.

The anti-dandruff shampoo comprises the following components in percentage by mass: 0.03% of D-glucosamine hydrochloride, 0.02% of scallop peptide, 0.02% of tuna protein peptide, 0.01% of salmon protein peptide, 0.01% of spirulina polysaccharide, 0.75% of piroctone olamine salt, 3% of PEG-150 distearate, 2% of glycerol, 0.1% of guar hydroxypropyltrimonium chloride, 0.05% of citric acid, 13% of PEG-80 sorbitan laurate, 0.5% of gamma-ionone, 150.1% of quaternary ammonium salt, 100.3% of polyquaternary ammonium salt, 25% of cocamidopropyl betaine, 20% of tridecyl alcohol sulfate sodium, 5.5% of disodium lauroamphodiacetate, 0.4% of tetrasodium EDTA, ethylene glycol distearate/laureth-4/cocamidopropyl betaine 8%, 0.75% of cyclopolydimethylsiloxane, and the balance of deionized water, which are available from Hubei Jusheng.

The anti-dandruff shampoo prepared by the embodiment has mild effect and small irritation, and can inhibit the growth of malassezia adiposa and relieve inflammation while effectively cleaning hair and scalp. After 2 weeks of continuous use, dandruff and pruritus symptoms disappear, and the hair becomes soft and easy to comb.

Example 4

Oyster peptide was obtained by the method of example 1.

The anti-dandruff shampoo comprises the following components in percentage by mass: d-glucosamine hydrochloride 0.02%, oyster peptide 0.025%, salmon protein peptide 0.04% purchased from Guangdong Xingyi ocean bioengineering GmbH, Hizikia fusiforme polysaccharide 0.02% purchased from Qingdao Bozhihui Biotech GmbH, Spirulina polysaccharide 0.02% purchased from SiAnbaichuan Biotech GmbH, piroctone olamine 0.2%, olive oil 0.5% purchased from Guangzhou Chengyi trade GmbH, panthenol 0.3% purchased from Hebei Baiwei Biotech GmbH, decyl glucoside 2%, PEG-160 sorbitan isostearate 1%, PEG-121%, glyceryl decanoate 1%, PEG-60 hydrogenated castor oil 0.5%, disodium laureth sulfosuccinate 8.2%, sodium PEG-4 laureth carboxylate 8%, Cocamet 3%, sodium carboxymethyl cocoyl polyallylamine 1%, sodium, 0.2% of bis (hydroxymethyl) imidazolidinyl urea, 0.2% of cis-jasmone, 0.01% of citric acid, 60.5% of polyquaternium and the balance of deionized water.

The anti-dandruff shampoo prepared by the embodiment has mild effect, good hair combing property after washing, no dryness and moderate cleaning power. After 2 weeks of continuous use, the scalp was no longer itchy and no dandruff was produced.

Example 5

Paphiaundulata peptide and oyster peptide were obtained by the method of example 1.

The anti-dandruff shampoo comprises the following components in percentage by mass: 0.04% of D-glucosamine hydrochloride, 0.01% of Paphia undulata peptide, 0.02% of oyster peptide, 0.05% of tuna protein peptide purchased from Shaanxi brocade bioengineering Co., Ltd, 0.03% of porphyra polysaccharide purchased from Qingdao Bozhi Biotech Co., Ltd, 0.02% of Hizikia fusiforme polysaccharide, 0.4% of piroctone olamine salt, 0.1% of olive oil, 0.5% of panthenol, 16% of ammonium lauryl sulfate, 6% of ammonium lauryl polyether-3 sulfate, 0.1% of BHT, 0.8% of carbomer, 13% of cocamide MEA, 2.1% of oleyl polyether-3 phosphate, 0.3% of guar hydroxypropyltrimonium chloride, 0.2% of phenoxyethyl isobutyrate, 8% of polysiloxane emulsion, 0.3% of mica/titanium dioxide, 0.1% of ethylene glycol distearate, 1% of propylene glycol, and the balance of deionized water.

The anti-dandruff shampoo prepared by the embodiment has mild effect and moderate foam, and the scalp feels comfortable and refreshing after washing. After 2 weeks of continuous use, the scalp was no longer greasy and itchy.

Example 6

Mussel peptide and pearl oyster peptide were obtained by the method of example 1.

The anti-dandruff shampoo comprises the following components in percentage by mass: d-glucosamine hydrochloride 0.025%, mussel peptide 0.02%, pearl oyster peptide 0.02%, tuna protein peptide 0.01%, salmon protein peptide 0.01%, laminarin 0.015% purchased from Sienna Baichuan Biotech Ltd, spirulina polysaccharide 0.01%, piroctone olamine salt 0.6%, olive oil 0.3%, panthenol 0.1%, sodium laureth-3 sulfate (28% by mass), essence 0.3%, quaternary ammonium salt-802%, hexyldecanol/ceramide-30.5%, phenoxyethanol/methylparaben/hydroxypropyl/hydroxybutyl ester 0.5%, cocamidopropyl betaine 7.3%, cocamidopropyl betaine/lauryl glucoside 5%, propylene glycol/PEG-55 propylene glycol oleate 0.8%, ethylene glycol stearate/steareth-43%, sodium chloride 1%, sodium chloride, 0.2 percent of p-tert-butyl cyclohexanol and the balance of deionized water.

The anti-dandruff shampoo prepared by the embodiment can effectively clean hair and scalp, and the scalp is comfortable and good in hair combing property after washing. After 2 weeks of continuous use, no dandruff is produced, and the hair quality is obviously improved.

Example 7

The anti-dandruff shampoos prepared in examples 1 to 6 were evaluated for anti-dandruff efficacy.

The test subjects were sample 1(A, a), sample 2(B, b), sample 3(C, c), sample 4(D, d), sample 5(E, e), sample 6(F, f). Samples a-F were in turn the anti-dandruff shampoos prepared in examples 1-6, and samples a-F were in turn control samples of samples a-F (samples a-F replaced the equivalent amount of deionized water for the mass percentages of D-glucosamine hydrochloride, marine shellfish peptide, fish protein peptide, algal polysaccharide, piroctone olamine, olive oil and panthenol in samples a-F, i.e. samples a-F did not contain D-glucosamine hydrochloride, marine shellfish peptide, fish protein peptide, algal polysaccharide, piroctone olamine, olive oil and panthenol).

36 volunteers with dandruff affliction, aged 18-48 years, were selected and randomly divided into 12 groups (i.e., samples A, a, B, B, C, C, D, D, E, E, F, F) of 3 individuals each. The corresponding samples were used to shampoo 1 time every 2 days for 4 consecutive weeks. And recording dandruff scores and pruritus scores on 0 day and 28 days after the samples are used, and determining the colony number of malassezia scalp so as to detect whether the samples have the effects of inhibiting bacteria, diminishing inflammation, removing dandruff and relieving itching.

Dividing scalp of each volunteer into 6 regions (front, middle and back regions on left and right sides respectively), and stickingThe ten-grade grading method for the adhesive scurf is used for grading the scurf, namely, the 0 grade represents no scurf, the 1-2 grade is small powdery grey coarse scale, the 3-4 grade is small to medium-sized chip, the 5-6 grade is large and thin chip connected with the loose scalp, the 7-8 grade is large adhesive chip, and the 9-10 grade is white to yellow thicker scale attached with the scalp tightly. And grading the pruritus by adopting an 8-grade pruritus grading method, wherein the grade 0 indicates no pruritus, the grade 1-2 indicates mild pruritus, the grade 3-4 indicates moderate pruritus, the grade 5-6 indicates severe pruritus, and the grade 7-8 indicates abnormal pruritus and inflammatory effusion. Scrubbing method is adopted, and the area of each zone is about 4cm²The area of the dandruff is taken, the dandruff is inoculated to a culture medium of a Sha dish containing 5 percent of sesame oil, the culture is carried out for 8 days at the constant temperature of 37 ℃, and the colony number of the malassezia is recorded. The dandruff scores of the volunteers are obtained by adding the dandruff scores of the regions, the pruritus scores of the volunteers are obtained by adding the pruritus scores of the regions, and the number of the malassezia colonies of the volunteers is obtained by adding the number of the malassezia colonies of the regions. The dandruff score and the pruritus score of each sample are average values of scores of corresponding groups of volunteers, and the number of malassezia colonies of each sample is the average value of the number of the malassezia colonies of the corresponding volunteers. The results of the experiment are shown in table 1.

Table 1: testing of bacteriostatic, anti-inflammatory, anti-dandruff and antipruritic efficacy of each sample

Compared with samples a, b, c, D, e and f, the results show that the dandruff score is improved by 2.83-3.13 times (about 17.4-19.0), the pruritus score is improved by 2.68-2.97 times (about 15.3-17.5), and the colony number of malassezia is improved by 5.58-7.77 times (25.2/cm) after the antidandruff shampoo containing D-glucosamine hydrochloride, marine shellfish peptide, fish protein peptide, algal polysaccharide and piroctone olamine is used²26.5 pieces/cm²). Scalp treated with test samples containing D-glucosamine hydrochloride, marine shellfish peptide, fish protein peptide, algal polysaccharide, piroctone olamine, olive oil, panthenol showed a 3.73-3.96-fold improvement in dandruff score (about 23.4-24.9), a 3.59-3.83-fold improvement in itch score (about 21.5-20.2), and horseThe colony number of the chromobacterium is improved by 5.03-7.88 times (25.0 colonies/cm)²-28.2 pieces/cm²). The composition contains oleum Olivarum and panthenol, and can be used for removing dandruff and relieving itching.

The anti-dandruff shampoo has the effects of inhibiting bacteria, diminishing inflammation, removing dandruff and relieving itching, the malassezia adiposa on the scalp is obviously reduced after the shampoo is used for 28 days, and the pruritus, the inflammation and the dandruff are obviously improved.

Example 8

The following 3 samples were subjected to antistatic and hair surface friction tests:

a hair composition base: ammonium laureth-3 sulfate 6.5%, ammonium laureth-3 sulfate 8.1%, sodium laureth sulfate 1.4%, cocamide MEA 1%, cetyl alcohol 0.35%, lauryl alcohol 0.2%, methyl [ bis- (hydrogenated tallow amidoethyl) ] -2-hydroxyethyl methylammonium sulfate 0.4%, trihydroxystearin 0.25%, sodium chloride 0.3%, ammonium xylene sulfonate 0.2%, citric acid 0.04%, sodium citrate 0.4%, sodium benzoate 0.25%, oleyl amidopropyl dimethylamine 0.1%, polydecene 0.4%, trimethylolpropane 0.1%, the balance of deionized water.

Sample a: contains D-glucosamine hydrochloride 0.01%, and hair composition matrix in balance.

③ sample B: contains D-glucosamine hydrochloride 0.05%, and hair composition matrix in balance.

D-glucosamine hydrochloride is purchased from Hubei Jusheng science and technology Co., Ltd, the relative humidity of the testing environment is 40% -60%, the tested hairs are 20cm long and all come from the same female volunteer, are aged 30 years, have long hairs and naturally have straight hairs (are not dyed).

The surface specific resistance tester is adopted, and the method for measuring the fabric softener by referring to national standards GB/T16801-1997 and GB/T14342-93 is adopted to evaluate the antistatic property of the hair by using the Delta lg rho s (surface specific resistance drop), wherein the larger the Delta lg rho s is, the better the antistatic property is.

The hair antistatic performance is evaluated by using an LDY type antistatic instrument according to an electrostatic performance measuring method of FZ/T01042-1996 textile material and using decay time T1/2(s), wherein the shorter the half-life period is, the better the antistatic performance is.

The friction factor mu of the hair after using the sample is measured by a Y151 type fiber friction factor measuring instrument, the friction resistance and the positive pressure thereof are called static friction factor mu s, and the average kinetic friction force and the positive pressure thereof are called kinetic friction factor mu d. The smaller the friction factor, the better the smoothness of the hair and the better the combing property.

Each sample was repeated 3 times and the average was taken. The results of the antistatic and hair surface friction tests for each sample are shown in table 2.

TABLE 2 antistatic and Hair surface Friction tests for each sample

After using the samples containing D-glucosamine hydrochloride (sample a, sample B), the hair surface specific resistance drop significantly increased, while the static half-life, the static friction factor and the kinetic friction factor significantly decreased. This shows that, after the addition of D-glucosamine hydrochloride, the antistatic properties of the hair are significantly improved and the surface friction is significantly reduced. That is, D-glucosamine hydrochloride has excellent film forming property on hair, can reduce static electricity generated by hair friction, can protect hair, repair damaged hair flakes, and improve smoothness, so that the hair is easy to comb.

Example 9

The following 7 samples were subjected to evaluation of bacteriostatic efficacy.

Malassezia furfur ATCC44344 was purchased from guangzhou norok instruments ltd (from guangdong collection of microbial cultures) and sterile distilled water was purchased from guangzhou drochen food and beverage ltd.

D-glucosamine hydrochloride, marine shellfish peptides, fish protein peptides and algal polysaccharides were prepared as in example 1.

Piroctone olamine salt aqueous solution (the content is 0.5 percent by mass, and the balance is sterile distilled water)

② aqueous solution containing D-glucosamine hydrochloride, marine shellfish peptide, fish protein peptide, algal polysaccharide, olive oil and panthenol (namely sample A, the total content is 0.5 percent by mass, the rest is sterile distilled water)

A1: 0.05% of D-glucosamine hydrochloride, 0.05% of oyster peptide, 0.05% of tuna protein peptide, 0.02% of porphyra polysaccharide, 0.03% of laminarin, 0.2% of olive oil, 0.1% of panthenol and the balance of sterile distilled water;

a2: 0.01% of D-glucosamine hydrochloride, 0.01% of Paphia undulata peptide, 0.01% of pearl oyster peptide, 0.02% of salmon protein peptide, 0.01% of sargassum fusiforme polysaccharide, 0.14% of olive oil, 0.3% of panthenol and the balance of sterile distilled water;

a3: 0.03% of D-glucosamine hydrochloride, 0.01% of scallop peptide, 0.01% of mussel peptide, 0.02% of tuna protein peptide, 0.02% of salmon protein peptide, 0.05% of spirulina polysaccharide, 0.16% of olive oil, 0.2% of panthenol and the balance of sterile distilled water.

③ D-glucosamine hydrochloride, marine shellfish peptide, fish protein peptide, algal polysaccharide, piroctone olamine, olive oil and panthenol aqueous solution (namely sample B, the total content is 0.5 percent by mass, the balance is sterile distilled water)

B1: 0.01% of D-glucosamine hydrochloride, 0.05% of oyster peptide, 0.03% of tuna protein peptide, 0.01% of porphyra polysaccharide, 0.02% of laminarin, 0.18% of piroctone olamine, 0.1% of olive oil, 0.1% of panthenol and the balance of sterile distilled water;

b2: 0.05% of D-glucosamine hydrochloride, 0.01% of Paphia undulata peptide, 0.01% of pearl oyster peptide, 0.01% of salmon protein peptide, 0.02% of sargassum fusiforme polysaccharide, 0.1% of piroctone olamine salt, 0.2% of olive oil, 0.1% of panthenol and the balance of sterile distilled water;

b3: 0.03% of D-glucosamine hydrochloride, 0.02% of scallop peptide, 0.03% of mussel peptide, 0.02% of tuna protein peptide, 0.03% of salmon protein peptide, 0.05% of spirulina polysaccharide, 0.12% of piroctone olamine salt, 0.1% of olive oil, 0.1% of panthenol and the balance of sterile distilled water.

Preparing a sample: and (3) taking the sterile dry filter paper, respectively dropwise adding 20 mu L of sample solution, flatly placing the sterile dry filter paper in a clean sterile empty plate, and naturally drying the sterile dry filter paper at room temperature in a biological safety cabinet for later use. The negative control group was distilled water.

Bacteriostatic experiments: inoculating the revived and identified Malassezia furfur ATCC44344 generation 3 to a Leeming-Hotman culture medium plate, and culturing at 30 ℃ for 24-48 h. Selecting bacterial colony, preparing into bacterial suspension with sterile normal saline, and dipping with sterile cotton swab to obtain 1 × 10⁴cfu/mL-9×10⁴cfu/mL Malassezia furfur ATCC44344 suspension was evenly spread on the surface of a Leeming-Hotman medium plate 3 times. Each application was done 1 time, the plate was rotated 60 °, and finally a cotton swab was applied around the edge of the plate for 1 week. The plate was covered and dried in a biosafety cabinet for 5 min. Subsequently, a sample piece was attached to the surface of the plate with sterile forceps, with the center of each piece being more than 25mm and the periphery of the plate being more than 15mm apart. After the sample is placed, the sample is lightly pressed by using sterile tweezers to make the sample tightly attached to the surface of the flat plate. And covering the plate, placing the plate in a mould incubator at 30 ℃ for culturing for 48 hours, and measuring the diameter of the bacteriostatic ring by using a vernier caliper and recording. Each sample was tested in 3 replicates and the results are shown in table 3.

Table 3: diameter of bacteriostatic circle (mm) for each sample

As can be seen from table 3, the diameter of the zone of inhibition of sample B is also 5% of the active ingredient, which is significantly larger than the diameter of the zone of inhibition of the bacteriostatic agent piroctone olamine salt aqueous solution, indicating that although piroctone olamine salt is often used as an antidandruff agent to inhibit the growth of microorganisms, its bacteriostatic effect is limited when it is used alone, but the invention adopts D-glucosamine hydrochloride, marine shellfish peptide, fish protein peptide, algal polysaccharide, olive oil and panthenol to significantly improve the bacteriostatic activity of piroctone olamine salt, suggesting that there is a good synergistic effect between them. In addition, in the active combination, the mass percentage of the piroctone olamine salt in the system is less than 0.2%, and the mass percentage of the piroctone olamine salt in the system is less than half of that of 0.5% of the piroctone olamine salt aqueous solution serving as the bacteriostatic agent, which also laterally reflects that in the composition, not only the piroctone olamine salt exerts bacteriostatic action, but also various components such as D-glucosamine hydrochloride, marine shellfish peptide, fish protein peptide and algal polysaccharide exert bacteriostatic action cooperatively as a whole.

Example 10

The following samples were subjected to evaluation of scalp and hair quality improving efficacy.

D-glucosamine hydrochloride, marine shellfish peptides, fish protein peptides and algal polysaccharides were prepared as in example 1.

Shampoo matrix: ammonium laureth-3 sulfate 6.5%, ammonium laureth-3 sulfate 8.1%, sodium laureth sulfate 1.4%, cocamide MEA 1%, cetyl alcohol 0.35%, lauryl alcohol 0.2%, methyl [ bis- (hydrogenated tallow amidoethyl) ] -2-hydroxyethyl methylammonium sulfate 0.4%, trihydroxystearin 0.25%, sodium chloride 0.3%, ammonium xylene sulfonate 0.2%, citric acid 0.04%, sodium citrate 0.4%, sodium benzoate 0.25%, oleyl amidopropyl dimethylamine 0.1%, polydecene 0.4%, trimethylolpropane 0.1%, the balance of deionized water.

② containing piroctone olamine salt (the content is 0.5 percent by mass, and the rest is shampoo matrix)

③ D-glucosamine hydrochloride, marine shellfish peptide, fish protein peptide and algal polysaccharide (namely sample A, the total content is 0.5 percent by mass, the rest is shampoo matrix)

A1: 0.05% of D-glucosamine hydrochloride, 0.15% of oyster peptide, 0.1% of tuna protein peptide, 0.1% of porphyra polysaccharide, 0.1% of laminarin and the balance of shampoo matrix;

a2: 0.05% of D-glucosamine hydrochloride, 0.1% of Paphia undulata peptide, 0.1% of pearl oyster peptide, 0.15% of salmon protein peptide, 0.1% of sargassum fusiforme polysaccharide and the balance of shampoo matrix;

a3: 0.1% of D-glucosamine hydrochloride, 0.1% of scallop peptide, 0.1% of mussel peptide, 0.05% of tuna protein peptide, 0.05% of salmon protein peptide, 0.1% of spirulina polysaccharide and the balance of shampoo matrix.

Fourthly, containing D-glucosamine hydrochloride, marine shellfish peptide, fish protein peptide, algal polysaccharide and piroctone olamine salt (namely sample B, the total content is 0.5 percent of mass fraction, and the balance is shampoo matrix)

B1: 0.01% of D-glucosamine hydrochloride, 0.05% of oyster peptide, 0.03% of tuna protein peptide, 0.01% of porphyra polysaccharide, 0.02% of laminarin, 0.38% of piroctone olamine salt and the balance of shampoo matrix;

b2: 0.05% of D-glucosamine hydrochloride, 0.01% of Paphia undulata peptide, 0.01% of pearl oyster peptide, 0.01% of salmon protein peptide, 0.02% of sargassum fusiforme polysaccharide, 0.4% of piroctone olamine salt and the balance of shampoo matrix;

b3: 0.03% of D-glucosamine hydrochloride, 0.02% of scallop peptide, 0.03% of mussel peptide, 0.02% of tuna protein peptide, 0.03% of salmon protein peptide, 0.05% of spirulina polysaccharide, 0.32% of piroctone olamine salt and the balance of shampoo matrix.

Fifthly, olive oil and panthenol (sample C, the total content is 0.5 percent by mass, and the balance is shampoo matrix)

C1: olive oil 0.3%, panthenol 0.2%, and shampoo matrix in balance;

c2: olive oil 0.4%, panthenol 0.1%, and shampoo matrix in balance.

Sixthly, the shampoo matrix contains D-glucosamine hydrochloride, marine shellfish peptide, fish protein peptide, algal polysaccharide, piroctone olamine salt, olive oil and panthenol (namely, the sample D, the total content is 0.5 percent of the mass fraction, and the balance is the shampoo matrix)

D1: 0.01% of D-glucosamine hydrochloride, 0.05% of oyster peptide, 0.03% of tuna protein peptide, 0.01% of porphyra polysaccharide, 0.02% of laminarin, 0.18% of piroctone olamine, 0.1% of olive oil, 0.1% of panthenol and the balance of shampoo matrix;

d2: 0.05% of D-glucosamine hydrochloride, 0.01% of Paphia undulata peptide, 0.01% of pearl oyster peptide, 0.01% of salmon protein peptide, 0.02% of sargassum fusiforme polysaccharide, 0.1% of piroctone olamine salt, 0.2% of olive oil, 0.1% of panthenol and the balance of shampoo matrix;

d3: 0.03% of D-glucosamine hydrochloride, 0.02% of scallop peptide, 0.03% of mussel peptide, 0.02% of tuna protein peptide, 0.03% of salmon protein peptide, 0.05% of spirulina polysaccharide, 0.12% of piroctone olamine salt, 0.1% of olive oil, 0.1% of panthenol and the balance of shampoo matrix.

39 volunteers, 21 men and 18 women, aged 25-52 years, without psoriasis on head skin, atopic dermatitis and other clinically diagnosed diseases were selected as subjects. The group was randomly divided into 13 groups, i.e., shampoo base group, piroctone olamine salt group, sample a group (sample a1-A3, containing D-glucosamine hydrochloride, marine shellfish peptide, fish protein peptide and algal polysaccharide), sample B group (sample B1-B3, containing D-glucosamine hydrochloride, marine shellfish peptide, fish protein peptide, algal polysaccharide and piroctone olamine), sample C group (sample C1-C2, containing olive oil and panthenol), sample D (sample D1-D3, containing D-glucosamine hydrochloride, marine shellfish peptide, fish protein peptide, algal polysaccharide, piroctone olamine, olive oil and panthenol), and 3 names per group. The hair is washed 1 time every 2 days, if other hair cosmetics are used, the hair-dandruff-resisting effect cannot be achieved, and the hair-dandruff-resisting shampoo is continuously used for 4 weeks.

Comparing the samples used for 0 day and 28 days, the oil content and the water content of the cuticle, the hair friction factor and the hair unit glossiness difference are detected to detect the improvement effect of each sample on the scalp and the hair. Oil and moisture contents of scalp of the testee were measured by using a multi-probe multi-functional skin tester MPA 10 (produced by CK company, germany), respectively. The hair friction factor mu is measured by a Y151 type fiber friction factor measuring instrument, and the smaller the value, the better the hair smoothness and the better the combing property. The hair gloss is measured using a hair glosser, with higher values indicating better hair gloss.

Each sample was repeated 3 times and the average was taken. Sample A is the average of samples A1-A3, sample B is the average of samples B1-B3, sample C is the average of samples C1-C2, and sample D is the average of samples D1-D3. The test results for each sample are shown in table 4.

Table 4: evaluation of scalp and hair improving efficacy of each sample

Compared to the shampoo base group, after using the other samples for 4 weeks, the moisture content of the scalp cuticle was increased, the secretion of oil was decreased, and the hair became smooth and glossy.

Compared with the sample A (containing D-glucosamine hydrochloride, marine shellfish peptides, seaweed polysaccharides and piroctone olamine) containing the pyrrolone ethanolamine salt group, the sample B (containing D-glucosamine hydrochloride, marine shellfish peptides, fish protein peptides, seaweed polysaccharides and piroctone olamine) has more remarkable effects of increasing the moisture content of the stratum corneum of the scalp, reducing the secretion of oil and improving the hair quality. The result shows that the piroctone olamine salt, D-glucosamine hydrochloride, marine shellfish peptide, fish protein peptide and algal polysaccharide have good synergistic effect, and the anti-dandruff shampoo containing the substances has the effects of keeping moisture for a long time, balancing oil secretion and improving hair quality.

Compared with sample B (containing D-glucosamine hydrochloride, marine shellfish peptide, fish protein peptide, algal polysaccharide, and piroctone olamine) and sample C (containing olive oil and panthenol), sample D (containing D-glucosamine hydrochloride, marine shellfish peptide, fish protein peptide, algal polysaccharide, piroctone olamine, olive oil, and panthenol) increased the moisture content of the scalp cuticle, and improved the hair softness and luster effects most significantly. The results show that D-glucosamine hydrochloride, marine shellfish peptide, fish protein peptide, algal polysaccharide, piroctone olamine salt, olive oil and panthenol have good synergistic effects, and the anti-dandruff shampoo containing the substances has remarkable effects of lasting moisture retention, balancing oil secretion, repairing scalp stratum corneum barrier and improving hair quality.

Therefore, the anti-dandruff shampoo has obvious effects of moisturizing, balancing grease secretion, inhibiting bacteria, diminishing inflammation, removing dandruff, relieving itching, repairing stratum corneum barrier and improving hair quality.

The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention are intended to be included within the scope of the present invention.

Claims (11)

1. The composition is characterized by comprising the following components in parts by weight:

d-glucosamine hydrochloride 1-10

Marine shellfish peptides 1-10

Fish protein peptide 1-10

Algal polysaccharide 1-10

10-100 parts of piroctone olamine salt

Olive oil 10-100

Panthenol 10-100.

2. The composition according to claim 1, wherein the marine shellfish peptide is derived from one or a combination of Paphia undulata, Pteria margaritifera, scallop, mussel, and oyster.

3. A composition according to claim 1, wherein the fish protein peptide is derived from tuna and/or salmon.

4. The composition of claim 1, wherein the algal polysaccharides are derived from one or more of laver, hizikia fusiforme, kelp, spirulina, kelp, ascochyta, and fucus vesiculosus.

5. A composition according to any of claims 1 to 4, wherein the composition is used in the preparation of or as a hair care product.

6. The composition according to any one of claims 1 to 4, wherein said composition is used for the preparation or use as a shampoo.

7. A composition according to any one of claims 1 to 4, wherein the composition is for use in the preparation or as an anti-dandruff shampoo.

8. The composition of claim 6, wherein the shampoo comprises 0.01-0.1% by weight of D-glucosamine hydrochloride, 0.01-0.1% by weight of marine shellfish peptide, 0.01-0.1% by weight of fish protein peptide, 0.01-0.1% by weight of algal polysaccharide, and 0.1-1.0% by weight of piroctone olamine.

9. The composition according to claim 6, wherein the weight percentage of each component in the shampoo is as follows: 0.01-0.05% of D-glucosamine hydrochloride, 0.01-0.05% of marine shellfish peptide, 0.01-0.05% of fish protein peptide, 0.01-0.05% of algal polysaccharide and 0.1-0.75% of piroctone olamine salt.

10. The composition of claim 6, wherein the shampoo comprises 0.01-0.1 wt% of D-glucosamine hydrochloride, 0.01-0.1 wt% of marine shellfish peptide, 0.01-0.1 wt% of fish protein peptide, 0.01-0.1 wt% of algal polysaccharide, 0.1-1.0 wt% of piroctone olamine, 0.1-1.0 wt% of olive oil, and 0.1-1.0 wt% of panthenol.

11. The composition of claim 6, wherein the shampoo comprises 0.01-0.05% by weight of D-glucosamine hydrochloride, 0.01-0.05% by weight of marine shellfish peptide, 0.01-0.05% by weight of fish protein peptide, 0.01-0.05% by weight of algal polysaccharide, 0.1-0.75% by weight of piroctone olamine, 0.1-0.5% by weight of olive oil, and 0.1-0.5% by weight of panthenol.

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