CN102144462A - Cutting propagation method of Miscanthus giganteus - Google Patents
Cutting propagation method of Miscanthus giganteus Download PDFInfo
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- CN102144462A CN102144462A CN 201010575257 CN201010575257A CN102144462A CN 102144462 A CN102144462 A CN 102144462A CN 201010575257 CN201010575257 CN 201010575257 CN 201010575257 A CN201010575257 A CN 201010575257A CN 102144462 A CN102144462 A CN 102144462A
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Abstract
The invention provides a cutting propagation method of Miscanthus giganteus, which comprises the steps of: (1) cutting treatment of a mature stem of the Miscanthus giganteus: stripping the stem from a leaf sheath and cutting into segments of 10-12cm as shoots for cutting, and obliquely cutting into a substrate after induced hormone treatment of axillary bud; (2) germination and cultivation of the axillary bud: controlling the temperature of the substrate at high temperature through warming geothermal lines for inducing the axillary bud to germinate and come out of the soil, and cultivating the growth of the bud by controlling the day temperature/night temperature at 20-30 DEG C/15-20 DEG C respectively; and (3) rooting cultivation of the bud: transplanting the bud to a nutrition pot after the bud grows to 15-20 cm or more, paving the geothermal lines to control the temperature of the substrate and promoting the rooting of the bud. The method has the advantages of high propagation efficiency, robust cuttage root system and high transplanting survival rate, and is a mature technology suitable for commercial propagation of the Miscanthus giganteus.
Description
Technical field
(the cottage propagation technology of Miscanthus * giganteus) specifically is to utilize the ripe stem stalk in strange hilllock to expedite the emergence of axillalry bud and take root to the present invention relates to strange hilllock.By this propagation technique, solve the awns platymiscium and utilize the lower problem of division propagation efficient, every Cong Qi hilllock (15 stems) can obtain 100-120 strain cuttage seeding, expands that numerous reproductive efficiency is higher more than 100 times, realizes scale, the commercialization production of strange hilllock seedling.Belong to biological technical field.
Background technology
Strange hilllock is the grass family herbaceos perennial, happiness light, cold-resistant, anti-morning, waterlogging, strong stress resistance, in garden landscape, roadside greening, in checking winds and fixing drifting sand huge application potential is arranged, also can be used for (Ercoli such as feed, construction material, papermaking, generating, Field Crops Research, 1999,63:3-11).Strange hilllock is a triploid plant, and its parent is Miscanthus sacchariflorus (reed) and Miscanthus sinensis (awns) (Huisman, Industry Crop Production, 1997,6 (3): 353-366).The elongated sword shape that is of blade, long 70-96cm, wide 2.1-2.8cm, color is bright green; Stem is nodular, and hollow has 12-13 joint when ripe; Give birth on the panicle top, umbrella, and white, the late September heading, winter, deposited the place, does not tie seed (Michael Bullard, Landwards, 1996,51 (2): 12-15); Acrial part is withered at the beginning of 11 months.Under natural environment, strange hilllock is by the root-like stock breeding, and annual 4-5 month, 8-9 month plant height reached 3-4 rice from the root-like stock director plant that makes new advances.
Root-like stock and division propagation are adopted at present strange hilllock, and every clump only can be expanded numerous 3-4 doubly, can not satisfy market demand.Cuttage is the simple and easy to do vegetative propagation technique of widely using in producing, but gramineous herbaceous plant's stem stalk hollow, cuttage is difficult for successfully, the report of not odd so far hilllock cottage propagation success both at home and abroad.
Summary of the invention:
The objective of the invention is to set up strange hilllock cottage propagation technical system.
Content of the present invention comprises the processing method of inducing and taking root with strange hilllock ripe stem stalk axillalry bud.
The present invention sets up strange hilllock cottage propagation technical system, the reproductive efficiency height, and cuttage seeding root system stalwartness, the transplanting survival rate height is to be suitable for the commercial mature technology that expands numerous strange hilllock.
Cuttage breeding method of the present invention comprises:
(1) gather strange hilllock stem stalk September after, divide different parts (top, middle part and base portion) to be cut into segment cuttage in greenhouse by solar heat, matrix is vermiculite.
(2) strange hilllock stem stalk axillalry bud is induced and is treated to following method and selects one: A. methyl NAA 10-80mgL
-1Soak plugged ear base portion 24h; B. indolebutyric acid IBA 10-80mgL
-1, soak plugged ear base portion 24h.
(3) the control substrate temperature is selected one below being after the plugged ear cuttage: A. day temperature/night temperature is 20-25 ℃/15-20 ℃; B. day temperature/night temperature is 25-30 ℃/15-20 ℃; C. day temperature/night temperature is 30-35 ℃/15-20 ℃; D. day temperature/night temperature is 35-38 ℃/15-20 ℃.
(4) the newborn young shoot in strange hilllock grows to 15cm and carries out root induction when above, and substrate temperature is selected one below being: A. day temperature/night temperature is 20-25 ℃/15-20 ℃; B. day temperature/night temperature is 25-30 ℃/15-20 ℃; C. day temperature/night temperature is 30-35 ℃/15-20 ℃; D. day temperature/night temperature is 35-38 ℃/15-20 ℃.
The scheme of technical solution problem of the present invention divides following three steps to finish:
(1) shear treatment of the ripe stem stalk in strange hilllock: peel off leaf sheath, the segment that the stem stalk is cut into long 10-12cm is as plugged ear, and every section is stayed 1 joint, and joint stays 2-3cm down, is cut into angle, stays 7-8cm on the joint, is cut into flat mouth.The different parts that carries out cane during as plugged ear cane 9 joints are arranged, be divided into 3 sections of top, middle part and base portions.
(2) axillalry bud brings out: cuttage after axillalry bud is induced HORMONE TREATMENT, floor file hot line control substrate temperature.
(3) young shoot is cultivated: young shoot is unearthed and grows to 3cm when above, controll day temperature 20-30 ℃ of temperature/night/15-20 ℃, natural lighting, the maintenance relative air humidity is more than 70%.
(4) young shoot root induction: young shoot grows to 15-20cm when above, and it is moved to the last bore that the matrix of taking root is housed is in the nutritive cube of 8cm, substrate temperature in the floor file hot line control nutritive cube.Natural lighting keeps relative air humidity more than 70%.
The advantage of the inventive method is
1. be the cuttage breeding method that plugged ear has been set up the strange hilllock of awns platymiscium with ripe stem stalk, each stem stalk of strange hilllock can be cut into the 8-9 section, but every section equal Cheng Miao, and the division propagation method that adopts expands numerous efficient raising more than 30 times at present.
2. the strange hilllock cuttage breeding method of the method foundation is to utilize newborn young shoot to carry out root induction again after inducing axillalry bud to grow to 10-15cm earlier, and difference is taken root earlier with the other plant cuttage, the method that back induced bud is sprouted.
3. the method has promoted strange hilllock axillary bud sprouting and has been unearthed axillary bud sprouting rate height fast by 30-38 ℃ of control high temperature.
4. the strange hilllock cuttage breeding method set up of the method, cuttage seeding root system stalwartness, the transplanting survival rate height is to be suitable for commercial numerous mature technology that expands.
Embodiment:
Further illustrate the present invention in the following embodiments, this does not limit the scope of the invention.
Embodiment 1
On September 6th, 2009 is 2 years strange hilllocks of life of clip in base, Beijing Grass Industry ﹠. Environment Research Development Center Xiao Tang mountain, choose stalwartness, no damage by disease and insect, and stem is slightly originated as plugged ear for the healthy stem stalk of 1-1.5cm band axillalry bud.The segment that the stem stalk is cut into long 10-12cm is as plugged ear, and every section is stayed 1 joint, and joint stays 2-3cm down, is cut into angle, stays 7-8cm on the joint, is cut into flat mouth.Adopt water, NAA and IBA to soak vernalization and handle, soak 24h.Use 15mgL during July 13 cuttage
-1The NAA immersion treatment.Cutting medium is a vermiculite, before the cuttage vermiculite is irrigated, and guarantees that at duration of test the cuttage environment is moistening, and the cuttage spacing in the rows is 5-6cm, line-spacing 7-8cm.Covering plastic film on the cutting bed preserves moisture.By underground heat line traffic control matrix day temperature/night temperature be 30-35 ℃/15-20 ℃, relative air humidity is 60%-80%.Plugged ear is peeled off leaf sheath before soaking, and every processing 20 plugged ears repeat 3 times.After 80% stem stalk young shoot is unearthed behind the 10d, controll day, temperature/night temperature was 20-25 ℃/15 ℃, after cultivating 15-20d, young shoot grows to 15-20cm when above, it is moved to the last bore that the matrix of taking root is housed is in the nutritive cube of 8cm, in the floor file hot line control nutritive cube matrix day degree 20-25 ℃ of temperature/night/15-20 ℃, natural lighting keeps relative air humidity more than 70%.Investigating strange hilllock stem stalk axillary bud sprouting rate during last basin is 85.3%.The root induction temperature is that a day temperature/night temperature be 20-25 ℃/15-20 ℃, and to investigate the plant rooting rate be 88.9% to disposable lifting behind the cultivation 20d.
Embodiment 2
Carried out different parts stem segment cuttage on September 15th, 2009.Cane 9 joint is cut into 3 sections of top, middle part and base portions, and the cutting method of each plugged ear is with embodiment 2.Investigating strange hilllock stem stalk axillary bud sprouting rate during basin on the stem section of top is 62.1%.Disposable lifting investigation plant rooting rate is 92.5% behind the root induction 20d.Investigating strange hilllock stem stalk axillary bud sprouting rate during basin on the stem section of middle part is 92.3%.Disposable lifting investigation plant rooting rate is 92.5% behind the root induction 20d.Investigating strange hilllock stem stalk axillary bud sprouting rate during basin on the base portion stem section is 100%.Disposable lifting investigation plant rooting rate is 100% behind the root induction 20d.
Embodiment 3
The cuttage program is all undertaken by embodiment 1, and institute's difference is that the day temperature that axillalry bud induces/night temperature is 35-38 ℃/15-20 ℃.Investigating strange hilllock stem stalk axillary bud sprouting rate during last basin is 93.4%.Disposable lifting investigation plant rooting rate is 100% behind the root induction 20d.
Embodiment 4
The cuttage program is all undertaken by embodiment 1, and institute's difference is that the strange hilllock of the clip stem stalk time is on September 30th, 2009, and investigating strange hilllock stem stalk axillary bud sprouting rate during last basin is 98.4%.Disposable lifting investigation plant rooting rate is 100% behind the root induction 20d.
Embodiment 5
The cuttage program is all undertaken by embodiment 1, and institute's difference is that the clip strange hilllock cane time is on September 30th, 2009, and investigating strange hilllock stem stalk axillary bud sprouting rate during last basin is 98.4%.Disposable lifting investigation plant rooting rate is 100% behind the root induction 20d.
Embodiment 6
The cuttage program is all undertaken by embodiment 1, and institute's difference is that axillalry bud is induced and is treated to 30mgL
-1NAA.Investigating strange hilllock stem stalk axillary bud sprouting rate during last basin is 89.4%.Disposable lifting investigation plant rooting rate is 91.5% behind the root induction 20d.
Embodiment 7
The cuttage program is all undertaken by embodiment 1, and institute's difference is that axillalry bud is induced and is treated to 30mgL
-1IBA.Investigating strange hilllock stem stalk axillary bud sprouting rate during last basin is 94.6%.Disposable lifting investigation plant rooting rate is 97.5% behind the root induction 20d.
Embodiment 8
The cuttage program is all undertaken by embodiment 2, and institute's difference is that a young shoot day temperature/night temperature of taking root is 25-30 ℃/15-20 ℃.Disposable lifting investigation plant rooting rate is 98.3% behind the root induction 20d.
Claims (3)
1. the cuttage breeding method on the strange hilllock of awns platymiscium, this method comprises: the shear treatment of the ripe stem stalk in (1) strange hilllock; (2) strange hilllock ripe stem stalk axillalry bud is induced.
2. in accordance with the method for claim 1, it is characterized in that the ripe stem stalk in the strange hilllock of lifes in 2 years of late September clip, the HORMONE TREATMENT method that axillalry bud is induced is selected one below being: A. methyl NAA 10-80mg/L, soak 24; B. indolebutyric acid IBA 10-80mg/L soaks 24.
3. in accordance with the method for claim 1, it is characterized in that strange hilllock ripe stem stalk axillalry bud induces substrate temperature to select one below being: A. day temperature/night temperature is 30-35 ℃/15-20 ℃; B. day temperature/night temperature is 35-38 ℃/15-20 ℃.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103875430A (en) * | 2014-04-08 | 2014-06-25 | 中国科学院成都生物研究所 | Method for vegetative propagation seedling cultivation of Miscamthusszechuanesis Keng rhizomes in arid region |
| CN109699323A (en) * | 2019-01-29 | 2019-05-03 | 滨州职业学院 | A kind of method of Huanghe delta autumn Chinese silvergrass breeding |
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| CN101248756A (en) * | 2008-01-23 | 2008-08-27 | 北京市农林科学院 | Genus Miscanthus Miscanthus giganteus tissue culture propagations method |
| CN101889552A (en) * | 2010-08-23 | 2010-11-24 | 湖南农业大学 | Rapid breeding method of miscanthus sinensis |
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2010
- 2010-12-07 CN CN 201010575257 patent/CN102144462A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101248756A (en) * | 2008-01-23 | 2008-08-27 | 北京市农林科学院 | Genus Miscanthus Miscanthus giganteus tissue culture propagations method |
| CN101889552A (en) * | 2010-08-23 | 2010-11-24 | 湖南农业大学 | Rapid breeding method of miscanthus sinensis |
Non-Patent Citations (3)
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| 《Plant Cell, Tissue and Organ Culture》 19950531 Nielsen JM 等 Synergism of thidizauron and benzyladenine in axillary shootformation depends on sequence of application in MiscanthusX ogiformis ＇Giganteus＇ 165-170 1-3 第41卷, * |
| 《中国优秀博硕士学位论文全文数据库（硕士）》 20050915 陈慧萍 奇岗无性繁殖技术研究 第12,30页 1-3 , 第5期 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103875430A (en) * | 2014-04-08 | 2014-06-25 | 中国科学院成都生物研究所 | Method for vegetative propagation seedling cultivation of Miscamthusszechuanesis Keng rhizomes in arid region |
| CN103875430B (en) * | 2014-04-08 | 2016-06-01 | 中国科学院成都生物研究所 | A kind of arid district river awns root stock asexual multiplication seedling method |
| CN109699323A (en) * | 2019-01-29 | 2019-05-03 | 滨州职业学院 | A kind of method of Huanghe delta autumn Chinese silvergrass breeding |
| CN109699323B (en) * | 2019-01-29 | 2021-07-02 | 滨州职业学院 | Method for breeding miscanthus sinensis in autumn of yellow river delta |
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Application publication date: 20110810 |