CN102119222B - 多核苷酸引物 - Google Patents

多核苷酸引物 Download PDF

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Publication number
CN102119222B
CN102119222B CN200880110038.7A CN200880110038A CN102119222B CN 102119222 B CN102119222 B CN 102119222B CN 200880110038 A CN200880110038 A CN 200880110038A CN 102119222 B CN102119222 B CN 102119222B
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China
Prior art keywords
primer
sequence
seq
dna
forward primer
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Expired - Fee Related
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CN200880110038.7A
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English (en)
Chinese (zh)
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CN102119222A (zh
Inventor
R·宝德
J·弗格逊
P·F·帕维托
N·J·德尔威尔
D·怀特科比
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DXS Ltd
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DXS Ltd
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Priority to CN201310752451.6A priority Critical patent/CN103695558B/zh
Priority to CN201310752306.8A priority patent/CN103834724B/zh
Publication of CN102119222A publication Critical patent/CN102119222A/zh
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Publication of CN102119222B publication Critical patent/CN102119222B/zh
Expired - Fee Related legal-status Critical Current
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN200880110038.7A 2007-09-28 2008-09-29 多核苷酸引物 Expired - Fee Related CN102119222B (zh)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN201310752451.6A CN103695558B (zh) 2007-09-28 2008-09-29 多核苷酸引物
CN201310752306.8A CN103834724B (zh) 2007-09-28 2008-09-29 多核苷酸引物

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0719034A GB2453173A (en) 2007-09-28 2007-09-28 Polynucleotide primers
GB0719034.1 2007-09-28
PCT/GB2008/003306 WO2009040557A2 (en) 2007-09-28 2008-09-29 Polynucleotide primers for detecting pik3ca mutations

Related Child Applications (3)

Application Number Title Priority Date Filing Date
CN201310752245.5A Division CN103952466B (zh) 2007-09-28 2008-09-29 多核苷酸引物
CN201310752306.8A Division CN103834724B (zh) 2007-09-28 2008-09-29 多核苷酸引物
CN201310752451.6A Division CN103695558B (zh) 2007-09-28 2008-09-29 多核苷酸引物

Publications (2)

Publication Number Publication Date
CN102119222A CN102119222A (zh) 2011-07-06
CN102119222B true CN102119222B (zh) 2015-11-25

Family

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Family Applications (4)

Application Number Title Priority Date Filing Date
CN200880110038.7A Expired - Fee Related CN102119222B (zh) 2007-09-28 2008-09-29 多核苷酸引物
CN201310752245.5A Expired - Fee Related CN103952466B (zh) 2007-09-28 2008-09-29 多核苷酸引物
CN201310752306.8A Expired - Fee Related CN103834724B (zh) 2007-09-28 2008-09-29 多核苷酸引物
CN201310752451.6A Expired - Fee Related CN103695558B (zh) 2007-09-28 2008-09-29 多核苷酸引物

Family Applications After (3)

Application Number Title Priority Date Filing Date
CN201310752245.5A Expired - Fee Related CN103952466B (zh) 2007-09-28 2008-09-29 多核苷酸引物
CN201310752306.8A Expired - Fee Related CN103834724B (zh) 2007-09-28 2008-09-29 多核苷酸引物
CN201310752451.6A Expired - Fee Related CN103695558B (zh) 2007-09-28 2008-09-29 多核苷酸引物

Country Status (13)

Country Link
US (4) US8901285B2 (https=)
EP (7) EP2505673B1 (https=)
JP (1) JP5719593B2 (https=)
KR (4) KR101543215B1 (https=)
CN (4) CN102119222B (https=)
AU (1) AU2008303400B2 (https=)
BR (1) BRPI0817444A2 (https=)
CA (2) CA2700710C (https=)
ES (6) ES2532141T3 (https=)
GB (1) GB2453173A (https=)
MX (1) MX2010003486A (https=)
RU (1) RU2491289C2 (https=)
WO (1) WO2009040557A2 (https=)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2453173A (en) 2007-09-28 2009-04-01 Dxs Ltd Polynucleotide primers
CA2786696C (en) 2010-01-12 2021-05-25 Jill Detmer Oligonucleotides and methods for detecting pik3ca mutations
JP2013081450A (ja) * 2011-09-27 2013-05-09 Arkray Inc 多型検出用プローブ、多型検出方法、薬効判定方法及び多型検出用試薬キット
CN102453765B (zh) * 2011-11-03 2013-07-24 厦门艾德生物医药科技有限公司 Pik3ca基因驱动突变的检测探针、引物及试剂盒
AU2013241752B2 (en) 2012-03-29 2016-07-07 Novartis Ag Pharmaceutical diagnostic
CN102816839A (zh) * 2012-07-06 2012-12-12 杭州艾迪康医学检验中心有限公司 用于检测结直肠癌pik3ca基因热点突变位点的试剂盒
EP2711432A1 (en) * 2012-09-21 2014-03-26 Genomica S.A.U. Method for detection of BRAF and PI3K mutations
EP2971075B1 (en) * 2013-03-13 2018-08-15 Roche Diagnostics GmbH Methods and compositions for detecting mutations in the human pi3kca (pik3ca) gene
CN103773837A (zh) * 2013-06-25 2014-05-07 宁波有成生物医药科技有限公司 一种pik3ca基因突变荧光定量pcr检测试剂盒及检测方法
RU2549682C1 (ru) * 2013-10-21 2015-04-27 Общество с ограниченной ответственностью "БИОЧИП-ИМБ" Способ анализа соматических мутаций в гене pi3k с использованием lna-блокирующей мультиплексной пцр и последующей гибридизацией с олигонуклеотидным биологическим микрочипом (биочипом)
WO2015161274A1 (en) 2014-04-18 2015-10-22 Blueprint Medicines Corporation Pik3ca fusions
CN105274188A (zh) * 2014-05-29 2016-01-27 北京雅康博生物科技有限公司 Pik3ca基因突变检测试剂盒
DK3198026T3 (da) * 2014-08-07 2020-01-20 Pharmassist Ltd Fremgangsmåde til bestemmelse af pik3ca-mutationsstatus i en prøve
US12391985B2 (en) 2014-08-07 2025-08-19 Pharmassist Ltd Method of determining PIK3CA mutational status in a sample
US10276072B2 (en) * 2014-09-03 2019-04-30 Washme Properties, Llc Illuminated sign
CN105441533B (zh) * 2014-11-29 2019-07-05 上海赛安生物医药科技有限公司 Pik3ca基因突变检测体系及其试剂盒
CN104531875A (zh) * 2014-12-30 2015-04-22 宁波有成生物医药科技有限公司 一种MyD88基因突变荧光定量PCR检测试剂盒及检测方法
WO2025110738A1 (ko) * 2023-11-24 2025-05-30 주식회사 캐니캐티케어 반려동물의 pik3ca 유전자 돌연변이성 종양 검출용 조성물 및 이를 이용한 반려동물의 pik3ca 유전자 돌연변이성 종양 진단방법
KR20250078789A (ko) 2023-11-24 2025-06-04 주식회사 캐니캐티케어 반려동물의 pik3ca 유전자 돌연변이성 종양 검출용 조성물 및 이를 이용한 반려동물의 pik3ca 유전자 돌연변이성 종양 진단방법

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004048511A2 (en) * 2002-11-26 2004-06-10 Rosetta Genomics Ltd. Bioinformatically detectable group of novel viral regulatory genes and uses thereof
WO2007050465A2 (en) * 2005-10-24 2007-05-03 The Johns Hopkins University Improved methods for beaming

Family Cites Families (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IE61148B1 (en) 1988-03-10 1994-10-05 Ici Plc Method of detecting nucleotide sequences
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US6274327B1 (en) 1992-04-13 2001-08-14 Ludwig Institute For Cancer Research Polypeptides having kinase activity, their preparation and use
GB9208135D0 (en) 1992-04-13 1992-05-27 Ludwig Inst Cancer Res Polypeptides having kinase activity,their preparation and use
US5846824A (en) 1994-02-07 1998-12-08 Ludwig Institute For Cancer Research Polypeptides having kinase activity, their preparation and use
US20030225013A1 (en) * 2002-05-31 2003-12-04 Isis Pharmaceuticals Inc. Antisense modulation of phosphoinositide-3-kinase, regulatory subunit 4, p150 expression
US5847972A (en) 1993-09-24 1998-12-08 Eick; Stephen Gregory Method and apparatus for graphically analzying a log-file
US6043062A (en) 1995-02-17 2000-03-28 The Regents Of The University Of California Constitutively active phosphatidylinositol 3-kinase and uses thereof
US5948664A (en) 1996-02-29 1999-09-07 The Regents Of The University Of California PI 3-kinase polypeptides
WO1998020126A1 (en) * 1996-11-01 1998-05-14 Onyx Pharmaceuticals, Inc. Nucleotide sequences that encode phosphatidylinositol-3' kinase associated proteins and uses thereof
US6277563B1 (en) 1997-01-16 2001-08-21 The Regents Of The University Of California Genetic alterations associated with cancer
US5955277A (en) 1997-05-05 1999-09-21 Novo Nordisk A/S Mutant cDNA encoding the p85α subunit of phosphatidylinositol 3-kinase
US5994076A (en) * 1997-05-21 1999-11-30 Clontech Laboratories, Inc. Methods of assaying differential expression
US8101349B2 (en) 1997-12-23 2012-01-24 Novartis Vaccines And Diagnostics, Inc. Gene products differentially expressed in cancerous cells and their methods of use II
US6537751B1 (en) 1998-04-21 2003-03-25 Genset S.A. Biallelic markers for use in constructing a high density disequilibrium map of the human genome
EP1105474A2 (en) 1998-05-14 2001-06-13 Chiron Corporation Human genes and gene expression products v
GB9812768D0 (en) * 1998-06-13 1998-08-12 Zeneca Ltd Methods
US6274237B1 (en) * 1999-05-21 2001-08-14 Chisso Corporation Potentially crimpable composite fiber and a non-woven fabric using the same
CA2388924A1 (en) * 1999-10-26 2001-05-03 Joan M. Robbins Ribozyme therapy for the treatment of proliferative skin and eye diseases
US6812339B1 (en) * 2000-09-08 2004-11-02 Applera Corporation Polymorphisms in known genes associated with human disease, methods of detection and uses thereof
WO2002027028A1 (en) 2000-09-28 2002-04-04 Atairgin Technologies, Inc. A method of determining tumor characteristics by determining abnormal copy number or expression level of lipid-associated genes
USH2191H1 (en) * 2000-10-24 2007-06-05 Snp Consortium Identification and mapping of single nucleotide polymorphisms in the human genome
CN1358850A (zh) * 2000-12-13 2002-07-17 上海博德基因开发有限公司 一种新的多肽——人磷脂酰肌醇3(PtdIns 3)－激酶17.6和编码这种多肽的多核苷酸
CA2372542A1 (en) 2001-02-20 2002-08-20 Pfizer Products Inc. Transgenic animals containing a dominant negative mutant form of the p85 subunit of pi-3 kinase
US20030162174A1 (en) * 2001-06-11 2003-08-28 Sutherland John W. Detecting nucleic acid deletion sequences
WO2003025175A2 (fr) * 2001-09-17 2003-03-27 Molecular Engines Laboratories Sequences impliquees dans les phenomenes de suppression tumorale, reversion tumorale, apoptose et/ou resistance aux virus et leur utilisation comme medicaments
US20030182669A1 (en) 2002-03-19 2003-09-25 Rockman Howard A. Phosphoinositide 3-kinase mediated inhibition of GPCRs
US7955800B2 (en) 2002-06-25 2011-06-07 Advpharma Inc. Metastasis-associated gene profiling for identification of tumor tissue, subtyping, and prediction of prognosis of patients
ATE470722T1 (de) * 2002-07-19 2010-06-15 Althea Technologies Inc Strategien zur genexpressionsanalyse
RU2240350C1 (ru) * 2003-03-11 2004-11-20 НИИ онкологии им. проф. Н.Н.Петрова Минздрава РФ Способ идентификации генных мутаций и полиморфизмов
US20070054278A1 (en) 2003-11-18 2007-03-08 Applera Corporation Polymorphisms in nucleic acid molecules encoding human enzyme proteins, methods of detection and uses thereof
JP2007530017A (ja) 2004-03-02 2007-11-01 ザ ジョンズ ホプキンス ユニバーシティ ヒトの癌におけるｐｉｋ３ｃａ遺伝子の変異
JP2009523006A (ja) * 2004-05-07 2009-06-18 アプレラ コーポレイション 脈管疾患に関連する遺伝的多型、その検出方法および使用
US20050287570A1 (en) * 2004-05-26 2005-12-29 Wyeth Probe arrays for expression profiling of rat genes
MX2007009963A (es) * 2005-02-24 2007-09-26 Amgen Inc Mutaciones del receptor del factor de crecimiento epidermico.
WO2006094149A2 (en) 2005-03-01 2006-09-08 Exact Sciences Corporation Methods and compositions for detecting adenoma
WO2006097899A1 (en) * 2005-03-14 2006-09-21 Koninklijke Philips Electronics, N.V. MEASURING AND MONITORING QoS IN SERVICE DIFFERENTIATED WIRELESS NETWORKS
GB2424886A (en) * 2005-04-04 2006-10-11 Dxs Ltd Polynucleotide primers against epidermal growth factor receptor and method of detecting gene mutations
JP4477575B2 (ja) * 2005-12-14 2010-06-09 株式会社日立製作所 大腸がんの検査に使用する遺伝子セット
AU2007225238A1 (en) * 2006-03-10 2007-09-20 Wyeth Microarray for monitoring gene expression in multiple strains of Streptococcus pneumoniae
GB2453173A (en) 2007-09-28 2009-04-01 Dxs Ltd Polynucleotide primers
KR20100105648A (ko) 2007-12-03 2010-09-29 엔즌 파마슈티칼스, 인코포레이티드 Ｐｉｋ３ｃａ 발현 조절용 ｒｎａ 길항제 화합물

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004048511A2 (en) * 2002-11-26 2004-06-10 Rosetta Genomics Ltd. Bioinformatically detectable group of novel viral regulatory genes and uses thereof
WO2007050465A2 (en) * 2005-10-24 2007-05-03 The Johns Hopkins University Improved methods for beaming

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Publication number Publication date
GB0719034D0 (en) 2007-11-07
ES2531055T3 (es) 2015-03-10
MX2010003486A (es) 2010-08-09
US20160032408A1 (en) 2016-02-04
KR101543215B1 (ko) 2015-08-07
US20110027779A1 (en) 2011-02-03
CN103695558B (zh) 2016-01-20
EP2505671A1 (en) 2012-10-03
RU2010116772A (ru) 2011-11-10
EP2236627A8 (en) 2010-12-01
EP2505673A1 (en) 2012-10-03
CN102119222A (zh) 2011-07-06
US9863003B2 (en) 2018-01-09
EP2508624A1 (en) 2012-10-10
KR20150038660A (ko) 2015-04-08
CN103952466B (zh) 2016-07-06
KR20100101070A (ko) 2010-09-16
WO2009040557A2 (en) 2009-04-02
US8901285B2 (en) 2014-12-02
US10190171B2 (en) 2019-01-29
KR101543216B1 (ko) 2015-08-07
EP2239341A8 (en) 2010-12-01
EP2239341A1 (en) 2010-10-13
EP2205760A2 (en) 2010-07-14
CA3010064A1 (en) 2009-04-02
EP2508624B1 (en) 2015-01-14
EP2239341B1 (en) 2015-01-14
EP2236627A1 (en) 2010-10-06
ES2532141T3 (es) 2015-03-24
KR20150038661A (ko) 2015-04-08
US20140141425A1 (en) 2014-05-22
EP2505672A1 (en) 2012-10-03
ES2532225T3 (es) 2015-03-25
AU2008303400A1 (en) 2009-04-02
EP2505673B1 (en) 2017-02-01
AU2008303400B2 (en) 2013-09-26
US10196692B2 (en) 2019-02-05
RU2491289C2 (ru) 2013-08-27
CA2700710A1 (en) 2009-04-02
EP2205760B1 (en) 2012-06-27
JP2010539920A (ja) 2010-12-24
JP5719593B2 (ja) 2015-05-20
US20160040255A1 (en) 2016-02-11
KR20150038659A (ko) 2015-04-08
KR101548758B1 (ko) 2015-09-01
WO2009040557A3 (en) 2009-05-14
ES2531994T3 (es) 2015-03-23
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EP2505672B1 (en) 2015-01-14
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KR101543214B1 (ko) 2015-08-07
BRPI0817444A2 (pt) 2015-06-16
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