EP2236627A8 - Polynucleotide primers for detecting PIK3CA mutations - Google Patents
Polynucleotide primers for detecting PIK3CA mutations Download PDFInfo
- Publication number
- EP2236627A8 EP2236627A8 EP10158519A EP10158519A EP2236627A8 EP 2236627 A8 EP2236627 A8 EP 2236627A8 EP 10158519 A EP10158519 A EP 10158519A EP 10158519 A EP10158519 A EP 10158519A EP 2236627 A8 EP2236627 A8 EP 2236627A8
- Authority
- EP
- European Patent Office
- Prior art keywords
- polynucleotide primers
- pik3ca mutations
- detecting
- detecting pik3ca
- primers
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000035772 mutation Effects 0.000 title abstract 3
- 239000002157 polynucleotide Substances 0.000 title abstract 2
- 102000040430 polynucleotide Human genes 0.000 title abstract 2
- 108091033319 polynucleotide Proteins 0.000 title abstract 2
- 101000605639 Homo sapiens Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform Proteins 0.000 title 1
- 102100038332 Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform Human genes 0.000 title 1
- 101150063858 Pik3ca gene Proteins 0.000 abstract 1
- 241000239226 Scorpiones Species 0.000 abstract 1
- 230000000295 complement effect Effects 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 102200085639 rs104886003 Human genes 0.000 abstract 1
- 102200085641 rs121913273 Human genes 0.000 abstract 1
- 102200085788 rs121913279 Human genes 0.000 abstract 1
- 102200085789 rs121913279 Human genes 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Pathology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Hospice & Palliative Care (AREA)
- Oncology (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
A method of detecting the presence or absence of a mutation in the PIK3CA gene, wherein the mutation is one of H1047R, H1047L, E542K and E545K, and preferably ARMS primers are combined with Scorpion primers.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB0719034A GB2453173A (en) | 2007-09-28 | 2007-09-28 | Polynucleotide primers |
EP08806456A EP2205760B1 (en) | 2007-09-28 | 2008-09-29 | Polynucleotide primers |
Related Parent Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP08806456A Division EP2205760B1 (en) | 2007-09-28 | 2008-09-29 | Polynucleotide primers |
EP08806456.3 Division | 2008-09-29 |
Publications (3)
Publication Number | Publication Date |
---|---|
EP2236627A1 EP2236627A1 (en) | 2010-10-06 |
EP2236627A8 true EP2236627A8 (en) | 2010-12-01 |
EP2236627B1 EP2236627B1 (en) | 2015-01-14 |
Family
ID=38701889
Family Applications (7)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP12169604.1A Not-in-force EP2508624B1 (en) | 2007-09-28 | 2008-09-29 | Polynucleotide primers for detecting pik3ca mutations |
EP10158519.8A Not-in-force EP2236627B1 (en) | 2007-09-28 | 2008-09-29 | Polynucleotide primers for detecting PIK3CA mutations |
EP08806456A Not-in-force EP2205760B1 (en) | 2007-09-28 | 2008-09-29 | Polynucleotide primers |
EP12169594.4A Not-in-force EP2505672B1 (en) | 2007-09-28 | 2008-09-29 | Polynucleotide primers for detecting pik3ca mutations |
EP12169599.3A Not-in-force EP2505673B1 (en) | 2007-09-28 | 2008-09-29 | Polynucleotide primers for detecting PIK3CA mutations |
EP10158523.0A Not-in-force EP2239341B1 (en) | 2007-09-28 | 2008-09-29 | Polynucleotide primers for detecting PIK3CA mutations |
EP12169586.0A Not-in-force EP2505671B1 (en) | 2007-09-28 | 2008-09-29 | Polynucleotide primers for detecting pik3ca mutations |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP12169604.1A Not-in-force EP2508624B1 (en) | 2007-09-28 | 2008-09-29 | Polynucleotide primers for detecting pik3ca mutations |
Family Applications After (5)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP08806456A Not-in-force EP2205760B1 (en) | 2007-09-28 | 2008-09-29 | Polynucleotide primers |
EP12169594.4A Not-in-force EP2505672B1 (en) | 2007-09-28 | 2008-09-29 | Polynucleotide primers for detecting pik3ca mutations |
EP12169599.3A Not-in-force EP2505673B1 (en) | 2007-09-28 | 2008-09-29 | Polynucleotide primers for detecting PIK3CA mutations |
EP10158523.0A Not-in-force EP2239341B1 (en) | 2007-09-28 | 2008-09-29 | Polynucleotide primers for detecting PIK3CA mutations |
EP12169586.0A Not-in-force EP2505671B1 (en) | 2007-09-28 | 2008-09-29 | Polynucleotide primers for detecting pik3ca mutations |
Country Status (13)
Country | Link |
---|---|
US (4) | US8901285B2 (en) |
EP (7) | EP2508624B1 (en) |
JP (1) | JP5719593B2 (en) |
KR (4) | KR101543216B1 (en) |
CN (4) | CN102119222B (en) |
AU (1) | AU2008303400B2 (en) |
BR (1) | BRPI0817444A2 (en) |
CA (2) | CA2700710C (en) |
ES (6) | ES2531055T3 (en) |
GB (1) | GB2453173A (en) |
MX (1) | MX2010003486A (en) |
RU (1) | RU2491289C2 (en) |
WO (1) | WO2009040557A2 (en) |
Families Citing this family (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2453173A (en) | 2007-09-28 | 2009-04-01 | Dxs Ltd | Polynucleotide primers |
WO2011087928A1 (en) | 2010-01-12 | 2011-07-21 | Siemens Healthcare Diagnostics Inc. | Oligonucleotides and methods for detecting kras and pik3ca mutations |
JP2013081450A (en) * | 2011-09-27 | 2013-05-09 | Arkray Inc | Probe for detecting polymorphism, method of detecting polymorphism, method of evaluating efficacy of drug, and reagent kit for detecting polymorphism |
CN102453765B (en) * | 2011-11-03 | 2013-07-24 | 厦门艾德生物医药科技有限公司 | Probe, primer and kit for detecting drive mutation of PIK3CA (Phosphatidylinositol-3-kinases) gene |
EP2830621B1 (en) | 2012-03-29 | 2020-10-21 | Novartis AG | Pharmaceutical diagnostic |
CN102816839A (en) * | 2012-07-06 | 2012-12-12 | 杭州艾迪康医学检验中心有限公司 | Kit for detecting hot spot mutation sites in colorectal cancer PIK3CA gene |
EP2711432A1 (en) * | 2012-09-21 | 2014-03-26 | Genomica S.A.U. | Method for detection of BRAF and PI3K mutations |
US20140272961A1 (en) * | 2013-03-13 | 2014-09-18 | Roche Molecular Systems, Inc. | Methods and compositions for detecting mutations in the human pi3kca (pik3ca) gene |
CN103773837A (en) * | 2013-06-25 | 2014-05-07 | 宁波有成生物医药科技有限公司 | Fluorescent quantitation PCR detection kit and detection method for PIK3CA (phosphatidylinositol3-kinase catalytic alpha) gene mutations |
RU2549682C1 (en) * | 2013-10-21 | 2015-04-27 | Общество с ограниченной ответственностью "БИОЧИП-ИМБ" | Method for analysis of somatic mutations in pi3k gene using lna-blocking multiplex pcr and subsequent hybridisation with oligonucleotide biological microchip (biochip) |
EP3132056B1 (en) | 2014-04-18 | 2021-11-24 | Blueprint Medicines Corporation | Pik3ca fusions |
CN105274188A (en) * | 2014-05-29 | 2016-01-27 | 北京雅康博生物科技有限公司 | PIK3CA gene mutation detection kit |
CA2957396C (en) * | 2014-08-07 | 2021-03-09 | Evrykleia LIANIDOU | Method of determining pik3ca mutational status in a sample |
US10276072B2 (en) * | 2014-09-03 | 2019-04-30 | Washme Properties, Llc | Illuminated sign |
CN105441533B (en) * | 2014-11-29 | 2019-07-05 | 上海赛安生物医药科技有限公司 | PIK3CA detection in gene mutation system and its kit |
CN104531875A (en) * | 2014-12-30 | 2015-04-22 | 宁波有成生物医药科技有限公司 | Fluorescent quantitative PCR detection kit and detection method for MyD88 gene mutation |
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-
2007
- 2007-09-28 GB GB0719034A patent/GB2453173A/en not_active Withdrawn
-
2008
- 2008-09-29 EP EP12169604.1A patent/EP2508624B1/en not_active Not-in-force
- 2008-09-29 EP EP10158519.8A patent/EP2236627B1/en not_active Not-in-force
- 2008-09-29 EP EP08806456A patent/EP2205760B1/en not_active Not-in-force
- 2008-09-29 EP EP12169594.4A patent/EP2505672B1/en not_active Not-in-force
- 2008-09-29 EP EP12169599.3A patent/EP2505673B1/en not_active Not-in-force
- 2008-09-29 ES ES12169604T patent/ES2531055T3/en active Active
- 2008-09-29 CA CA2700710A patent/CA2700710C/en not_active Expired - Fee Related
- 2008-09-29 KR KR1020157006584A patent/KR101543216B1/en active IP Right Grant
- 2008-09-29 KR KR1020157006583A patent/KR101543215B1/en active IP Right Grant
- 2008-09-29 JP JP2010526365A patent/JP5719593B2/en active Active
- 2008-09-29 RU RU2010116772/10A patent/RU2491289C2/en not_active IP Right Cessation
- 2008-09-29 ES ES12169599.3T patent/ES2620289T3/en active Active
- 2008-09-29 CA CA3010064A patent/CA3010064A1/en not_active Abandoned
- 2008-09-29 KR KR1020157006582A patent/KR101543214B1/en active IP Right Grant
- 2008-09-29 MX MX2010003486A patent/MX2010003486A/en active IP Right Grant
- 2008-09-29 KR KR1020107009180A patent/KR101548758B1/en active IP Right Grant
- 2008-09-29 BR BRPI0817444-0A patent/BRPI0817444A2/en active Search and Examination
- 2008-09-29 CN CN200880110038.7A patent/CN102119222B/en not_active Expired - Fee Related
- 2008-09-29 ES ES10158519.8T patent/ES2532141T3/en active Active
- 2008-09-29 ES ES10158523.0T patent/ES2531994T3/en active Active
- 2008-09-29 WO PCT/GB2008/003306 patent/WO2009040557A2/en active Application Filing
- 2008-09-29 EP EP10158523.0A patent/EP2239341B1/en not_active Not-in-force
- 2008-09-29 EP EP12169586.0A patent/EP2505671B1/en not_active Not-in-force
- 2008-09-29 ES ES12169586.0T patent/ES2622885T3/en active Active
- 2008-09-29 AU AU2008303400A patent/AU2008303400B2/en not_active Ceased
- 2008-09-29 US US12/680,621 patent/US8901285B2/en not_active Expired - Fee Related
- 2008-09-29 CN CN201310752306.8A patent/CN103834724B/en not_active Expired - Fee Related
- 2008-09-29 CN CN201310752245.5A patent/CN103952466B/en not_active Expired - Fee Related
- 2008-09-29 ES ES12169594.4T patent/ES2532225T3/en active Active
- 2008-09-29 CN CN201310752451.6A patent/CN103695558B/en not_active Expired - Fee Related
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2013
- 2013-10-24 US US14/062,163 patent/US9863003B2/en not_active Expired - Fee Related
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2015
- 2015-09-18 US US14/858,188 patent/US10196692B2/en not_active Expired - Fee Related
- 2015-09-18 US US14/858,196 patent/US10190171B2/en not_active Expired - Fee Related
Also Published As
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Publication | Publication Date | Title |
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