CN102058020B - Chinese medicinal herb additive for improving immunity of juvenile sea cucumber - Google Patents
Chinese medicinal herb additive for improving immunity of juvenile sea cucumber Download PDFInfo
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- CN102058020B CN102058020B CN2009102197916A CN200910219791A CN102058020B CN 102058020 B CN102058020 B CN 102058020B CN 2009102197916 A CN2009102197916 A CN 2009102197916A CN 200910219791 A CN200910219791 A CN 200910219791A CN 102058020 B CN102058020 B CN 102058020B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention discloses a Chinese medicinal herb additive for improving immunity of juvenile sea cucumber. Astragalus serves as a main raw material, and is added with codonopsis pilosula, eucommia bark, corydalis bungeana, areca seed, tangerine peel, bupleurum, fortune eupatorium herb, ash bark, szechuan lovage rhizome, Chinese gall, baical skullcap root, lightyellow sophora root, garlic, indigowoad leaf, honeysuckle, dutchmanspipe fruit, berberis, rhubarb, beta-glucan, yeast, mannan oligosaccharide and the like to prepare a Chinese medicinal herb feed additive for promoting immunity. The additive has high attraction on sea cucumber, and has the advantages of no pollution, no residue, no medicament resistance, no side effect and the like. When the additive is added into a common feed to feed the sea cucumber, the additive can promote immune cell differentiation, maturity and multiplication of immune tissues of the sea cucumber so as to promote the immunity of the sea cucumber. Therefore, the sea cucumber has high survival rate, high weight increment speed, and the juvenile sea cucumber has fast color change and high extension, and the additive has an obvious effect of protecting the juvenile sea cucumber.
Description
Technical field:
The present invention relates to a kind of feed addictive, especially a kind of pollution-free, noresidue, the no resistance to the action of a drug, have no side effect, can improve survival rate of Apostichopus japonicus, increase children's ginseng body weight fast, impel children's ginseng thorn seedling that look accelerates, range of extension is good immunity Chinese herb feed additive.
Background technology:
The sea cucumber body contains more than the 50 kind of nutrition useful to the human physiological activity; Its protein contains 18 seed amino acids, is rich in multivitamins such as element, vitamin B1, vitamin B2 and niacin such as various active materials such as taurine, chondroitin sulfate, stichopus japonicus mucopolysaccharide and calcium, phosphorus, iron, iodine, zinc, selenium, vanadium, manganese.The arginine that wherein can promote body cell regeneration, the impaired back of body to repair is the abundantest, and beche-de-mer can improve the immunologic function of human body, promotes longevity, eliminates tired.
Along with the significantly rising of sea cucumber demand, the holothruian cultures industry also develops rapidly.Exist a lot of problems in the holothruian cultures process at present, the most serious is exactly the disease problem of death that stings seedling.Some culture the dealer for preventing and treating disease, in the pond of culturing sea cucumber, add antibiotic; Add growth hormone etc. in the feed for promoting sea cucumber to be grown in fast,, in the sea cucumber body that but causes growing up medicament residue is arranged, directly endanger eater's health though can prevent and treat some disease.Nonetheless, some disease still can not suppress,, course of disease weak point anxious like the morbidity of stichopus japonicus Beancurd sheet syndrome, in case morbidity uses the antibiotic therapy effect also undesirable, the death rate is high.
Summary of the invention:
The present invention is in order to solve the above-mentioned technical problem of existing in prior technology; A kind of pollution-free, noresidue, the no resistance to the action of a drug are provided, have no side effect; Can improve survival rate of Apostichopus japonicus, increase children's ginseng body weight fast, impel children's ginseng thorn seedling that look accelerates, range of extension is good immunity Chinese herb feed additive.
Technical solution of the present invention is: a kind of thorn seedling immunity Chinese herb feed additive; Comprise following component, each components by weight is: the Radix Astragali 10～16, Radix Codonopsis 9～11, the bark of eucommia 7～12, honeysuckle 6～9, betel nut 4～7, dried orange peel 3～7, rheum officinale 3～8, folium isatidis 2～8, the bark of ash 2～5, Ligusticum wallichii 1～5, Chinese gall 1～7, the root of large-flowered skullcap 1～4, kuh-seng 1～3, garlic 1～5, eupatorium 0.6～1, corydlis bungeana 0.3～0.6, birthwort 0.2～0.7, barberry 0.1～0.6 and radix bupleuri 0.1～0.3.
Also comprise following component in the said component, by weight: beta glucan 0.03～0.06, yeast 0.02～0.05 and manna oligosacchride 0.01～0.04.
The weight ratio of said component is: 0.03 part of the Radix Astragali 10～12, Radix Codonopsis 9～11, the bark of eucommia 9～12, honeysuckle 6～9, betel nut 4～7, dried orange peel 5～7, rheum officinale 3～5, folium isatidis 2～4, the bark of ash 3～5, Ligusticum wallichii 1～4, Chinese gall 1～4, the root of large-flowered skullcap 2～4, kuh-seng 1～3, garlic 1～3, eupatorium 0.6～0.9, corydlis bungeana 0.3～0.5, birthwort 0.2～0.4, barberry 0.1～0.5,0.1～0.4 part of radix bupleuri, beta glucan 0.04, yeast 0.05 and manna oligosacchride.
The weight ratio of said component is: 0.02 part of the Radix Astragali 11～13, Radix Codonopsis 10～11, the bark of eucommia 9～10, honeysuckle 6～9, betel nut 5～7, dried orange peel 5～7, rheum officinale 3～6, folium isatidis 2～4, the bark of ash 3～5, Ligusticum wallichii 1～4, Chinese gall 2～4, the root of large-flowered skullcap 2～4, kuh-seng 2～3, garlic 1～3, eupatorium 0.6～0.9, corydlis bungeana 0.2～0.5, birthwort 0.2～0.5, barberry 0.1～0.5, radix bupleuri 0.2～0.4,0.03 part of beta glucan, 0.04 part in yeast and manna oligosacchride.
The weight ratio of said component is: 0.01 part of the Radix Astragali 12～16, Radix Codonopsis 9～10, the bark of eucommia 8～9, honeysuckle 6～8, betel nut 5～7, dried orange peel 6～7, rheum officinale 3～6, folium isatidis 2～4, the bark of ash 2～4, Ligusticum wallichii 2～4, Chinese gall 3～5, the root of large-flowered skullcap 2～4, kuh-seng 1～3, garlic 1～3, eupatorium 0.6～0.8, corydlis bungeana 0.3～0.5, birthwort 0.2～0.5, barberry 0.1～0.5, radix bupleuri 0.2～0.4, beta glucan 0.05, yeast 0.02 and manna oligosacchride.
The weight ratio of said component is: 0.04 part of the Radix Astragali 11～15, Radix Codonopsis 8～10, the bark of eucommia 7～9, honeysuckle 6～8, betel nut 5～7, dried orange peel 3～7, rheum officinale 3～6, folium isatidis 3～8, the bark of ash 2～4, Ligusticum wallichii 2～4, Chinese gall 3～7, the root of large-flowered skullcap 2～4, kuh-seng 1～3, garlic 2～5, eupatorium 0.6～0.8, corydlis bungeana 0.3～0.5, birthwort 0.3～0.7, barberry 0.1～0.3, radix bupleuri 0.2～0.4, beta glucan 0.06, yeast 0.03 and manna oligosacchride.
The present invention adopts the Radix Astragali to make main raw material; Add Radix Codonopsis, the bark of eucommia, corydlis bungeana, betel nut, dried orange peel, radix bupleuri, eupatorium, the bark of ash, Ligusticum wallichii, Chinese gall, the root of large-flowered skullcap, kuh-seng, garlic, folium isatidis, honeysuckle, birthwort, barberry, rheum officinale, beta glucan, yeast, manna oligosacchride etc. again and form the promoting immunity Chinese herbal feed additive; Attractant to stichopus japonicus is good, has pollution-free, noresidue, the no resistance to the action of a drug, advantage such as have no side effect; Be equipped with the normal diet sea cucumber of feeding with the present invention; Can promote the immunocyte differentiation of stichopus japonicus immuning tissue, ripe, propagation; Thereby the immunity of stichopus japonicus, the look that stichopus japonicus shoot survival percent height, fast gaining, children are joined accelerates, range of extension is good, and the protection seedling is had remarkable result.
The specific embodiment:
Embodiment 1:
Each component of raw material is by following weight ratio preparation, and each is below component ultramicro grinding to 300 order, and mixing gets final product.
The weight ratio of said component is: 0.03 part of the Radix Astragali 10～12, Radix Codonopsis 9～11, the bark of eucommia 9～12, honeysuckle 6～9, betel nut 4～7, dried orange peel 5～7, rheum officinale 3～5, folium isatidis 2～4, the bark of ash 3～5, Ligusticum wallichii 1～4, Chinese gall 1～4, the root of large-flowered skullcap 2～4, kuh-seng 1～3, garlic 1～3, eupatorium 0.6～0.9, corydlis bungeana 0.3～0.5, birthwort 0.2～0.4, barberry 0.1～0.5,0.1～0.4 part of radix bupleuri, beta glucan 0.04, yeast 0.05 and manna oligosacchride.
Embodiment 2:
Each component of raw material is by following weight ratio preparation, and each is below component ultramicro grinding to 300 order, and mixing gets final product.
The weight ratio of said component is: 0.02 part of the Radix Astragali 11～13, Radix Codonopsis 10～11, the bark of eucommia 9～10, honeysuckle 6～9, betel nut 5～7, dried orange peel 5～7, rheum officinale 3～6, folium isatidis 2～4, the bark of ash 3～5, Ligusticum wallichii 1～4, Chinese gall 2～4, the root of large-flowered skullcap 2～4, kuh-seng 2～3, garlic 1～3, eupatorium 0.6～0.9, corydlis bungeana 0.2～0.5, birthwort 0.2～0.5, barberry 0.1～0.5, radix bupleuri 0.2～0.4,0.03 part of beta glucan, 0.04 part in yeast and manna oligosacchride.
Embodiment 3:
Each component of raw material is by following weight ratio preparation, and each is below component ultramicro grinding to 300 order, and mixing gets final product.
The weight ratio of said component is: 0.01 part of the Radix Astragali 12～16, Radix Codonopsis 9～10, the bark of eucommia 8～9, honeysuckle 6～8, betel nut 5～7, dried orange peel 6～7, rheum officinale 3～6, folium isatidis 2～4, the bark of ash 2～4, Ligusticum wallichii 2～4, Chinese gall 3～5, the root of large-flowered skullcap 2～4, kuh-seng 1～3, garlic 1～3, eupatorium 0.6～0.8, corydlis bungeana 0.3～0.5, birthwort 0.2～0.5, barberry 0.1～0.5, radix bupleuri 0.2～0.4, beta glucan 0.05, yeast 0.02 and manna oligosacchride.
Embodiment 4:
Each component of raw material is by following weight ratio preparation, and each is below component ultramicro grinding to 300 order, and mixing gets final product.
The weight ratio of said component is: 0.04 part of the Radix Astragali 11～15, Radix Codonopsis 8～10, the bark of eucommia 7～9, honeysuckle 6～8, betel nut 5～7, dried orange peel 3～7, rheum officinale 3～6, folium isatidis 3～8, the bark of ash 2～4, Ligusticum wallichii 2～4, Chinese gall 3～7, the root of large-flowered skullcap 2～4, kuh-seng 1～3, garlic 2～5, eupatorium 0.6～0.8, corydlis bungeana 0.3～0.5, birthwort 0.3～0.7, barberry 0.1～0.3, radix bupleuri 0.2～0.4, beta glucan 0.06, yeast 0.03 and manna oligosacchride.
The pharmacological action of key component:
Radix Astragali another name: arrow stilbene, continuous stilbene, mouthful stilbene, casting skin stilbene, RADIX ASTRAGALI, red stilbene, only stilbene etc.Red stilbene is wild, and root is cylindrical, is the vertical bar shape mostly, rare branch, and the upper end is slightly thick, and the lower end is gradually thin, long 10～50cm, diameter 0.6～2cm; The grey rufous in surface, vertical wrinkle of tool and minority supporting root trace, cork is prone to come off and exposes flaxen skin zone and fiber; Hole skin is grown crosswise, and look shallow, and yellow or dark yellow are slightly outstanding; Matter is hard and tough, frangible not, and section shows fibroid and shows mealiness; Cross section skin zone yellow-white accounts for radius 1/2～1/3, forms layer light brown, the radial texture of the fallow tool of xylem; Gas is little, and it is sweet to distinguish the flavor of, and that chews has a beany flavor.Radix Astragali slightly warm in nature, it is sweet to distinguish the flavor of, and the effect of invigorating qi for strengthening superficies, hidroschesis detoxification, myogenic, diuresis, detumescence is arranged.Antivirus action: Sindbis virus, NDV (NDK), folliculus property stomatitis virus (VSV) and influenza virus are had antivirus action.Chemical composition: contain flavones ingredient calycosin (calycosin), 3-hydroxyl-9,10-dimethoxy red sandalwood alkane also contains astragalus saponin I, V, III (astragaloside I, V, III).
Through Radix Astragali assay result is shown that the Radix Astragali can be used as feed addictive fully.
Carry out the sea cucumber experiment of feeding in the experiment base: the experiment seawater also continues to inflate in the pond through filtering for the natural water area tide seawater that rises and falls, every day a quantity of circulating water.The ammonia nitrogen of experimental water, nitrite, hydrogen sulfide, PH, dissolved oxygen equivalence are all in normal range (NR).17～20 ℃ of water temperatures, salinity 29.5 ‰.Divide four zoness of different to compare test in same marine site; The experiment field is the industrial aquaculture booth; The contrast experiment is done with two cement pit A, B in each experiment field, and the normal diet that uses market to buy is is respectively thrown something and fed with equal number, the same terms with the identical feed that by mass ratio is 100: 1 adding embodiment of the invention.
Test totally 60 days, the 2h that stops eating before experiment finishes weighs, statistics survival rate, rate of body weight gain.Get 3 sea cucumbers at random for every group, dissect, get its coelomic fluid, muscle, centrifugal by the speed and the time of different detection required conditions of index and kit requirement.The employing automatic clinical chemistry analyzer is measured; Superoxide dismutase (SOD), MDA (MDA), alkaline phosphatase (AKP), acid phosphatase (ACP) adopt Nanjing to build up kit measurement that bio-engineering research is produced; SOD adopts xanthine oxidase; The mensuration of MDA adopts 2-thiobarbituricacid (TBA) colorimetric method to carry out, and AKP, ACP adopt colorimetric method.
Experiment place one: survival rate and weightening finish contrast experiment: cement pit: 3 * 4 * 1=12 cubic meter, young ginseng seedling 1.5kg.The survival rate experimental result is shown in table one, and the weightening finish experimental result is (the normal diet group is designated as the A group, and the feed group that adds the embodiment of the invention 1 is designated as the B group) shown in table two.
Table one
Table two
Table three
Chinese herb feed additive is to the influence (mk/kg of unit) of sea cucumber coelomic fluid immune indexes:
Table four
Chinese herb feed additive is to the influence (mk/kg of unit) of sea cucumber muscle immune indexes:
Experiment place two: survival rate and weightening finish contrast experiment: cement pit: 8 * 5 * 1=40 cubic meter, young ginseng seedling 6kg.The survival rate experimental result is shown in table five, and the weightening finish experimental result is (the normal diet group is designated as the A group, and the feed group that adds the embodiment of the invention 2 is designated as the B group) shown in table six:
Table five
Table six
Table seven
Chinese herb feed additive is to the influence (mk/kg of unit) of sea cucumber coelomic fluid immune indexes:
Table eight
Chinese herb feed additive is to the influence (mk/kg of unit) of sea cucumber muscle immune indexes:
Experiment place three: survival rate and weightening finish contrast experiment: cement pit: 8 * 5 * 1=40 cubic meter, young ginseng seedling 10kg.The survival rate experimental result is shown in table nine, and the weightening finish experimental result is (the normal diet group is designated as the A group, and the feed group that adds the embodiment of the invention 3 is designated as the B group) shown in table ten:
Table nine
Table ten
Table ten one
Chinese herb feed additive is to the influence (mk/kg of unit) of sea cucumber coelomic fluid immune indexes:
Table ten two
Chinese herb feed additive is to the influence (mk/kg of unit) of sea cucumber muscle immune indexes:
Experiment place four: survival rate and weightening finish contrast experiment: cement pit: 8 * 5 * 1=40 cubic meter, young ginseng seedling 13kg.The survival rate experimental result is shown in table ten three, and the weightening finish experimental result is (the normal diet group is designated as the A group, and the feed group that adds the embodiment of the invention 4 is designated as the B group) shown in table ten four:
Table ten three
Table ten four
Table ten five
Chinese herb feed additive is to the influence (mk/kg of unit) of sea cucumber coelomic fluid immune indexes:
Table ten six
Chinese herb feed additive is to the influence (mk/kg of unit) of sea cucumber muscle immune indexes:
Can know by experimental result; With the feed that the has added the embodiment of the invention young ginseng of feeding; SOD in the coelomic fluid, MDA, AKP, ACP compare significant difference (P＜0.05) with control group, activity all obviously raises, and the SOD in the muscle compares the also obviously rising of significant difference (P＜0.05) activity with control group; But MDA compares obviously change of remarkable (P＞0.05) active not generation of difference in the muscle with control group; Activity that the embodiment of the invention can be through improving antioxidase in the body is described improving immunity of organisms, and survival rate is high more than 30% than the young ginseng survival rate of feeding with general feeds, weightening finish is also very remarkable.This feed addictive is good to the attractant of young ginseng, and does not have residual material slag.It is very fast that the look of young ginseng becomes, and range of extension is good.The return rate of feed addictive of the present invention significantly is embodied in the survival rate aspect, to growing seedlings and keeping a full stand of seedings remarkable result is arranged.
Claims (4)
1. thorn seedling immunity Chinese herb feed additive is characterized in that the weight ratio of said component is: 0.03 part of the Radix Astragali 10～12, Radix Codonopsis 9～11, the bark of eucommia 9～12, honeysuckle 6～9, betel nut 4～7, dried orange peel 5～7, rheum officinale 3～5, folium isatidis 2～4, the bark of ash 3～5, Ligusticum wallichii 1～4, Chinese gall 1～4, the root of large-flowered skullcap 2～4, kuh-seng 1～3, garlic 1～3, eupatorium 0.6～0.9, corydlis bungeana 0.3～0.5, birthwort 0.2～0.4, barberry 0.1～0.5,0.1～0.4 part of radix bupleuri, beta glucan 0.04, yeast 0.05 and manna oligosacchride.
2. thorn seedling immunity Chinese herb feed additive is characterized in that the weight ratio of said component is: 0.02 part of the Radix Astragali 11～13, Radix Codonopsis 10～11, the bark of eucommia 9～10, honeysuckle 6～9, betel nut 5～7, dried orange peel 5～7, rheum officinale 3～6, folium isatidis 2～4, the bark of ash 3～5, Ligusticum wallichii 1～4, Chinese gall 2～4, the root of large-flowered skullcap 2～4, kuh-seng 2～3, garlic 1～3, eupatorium 0.6～0.9, corydlis bungeana 0.2～0.5, birthwort 0.2～0.5, barberry 0.1～0.5, radix bupleuri 0.2～0.4,0.03 part of beta glucan, 0.04 part in yeast and manna oligosacchride.
3. thorn seedling immunity Chinese herb feed additive is characterized in that the weight ratio of said component is: 0.01 part of the Radix Astragali 12～16, Radix Codonopsis 9～10, the bark of eucommia 8～9, honeysuckle 6～8, betel nut 5～7, dried orange peel 6～7, rheum officinale 3～6, folium isatidis 2～4, the bark of ash 2～4, Ligusticum wallichii 2～4, Chinese gall 3～5, the root of large-flowered skullcap 2～4, kuh-seng 1～3, garlic 1～3, eupatorium 0.6～0.8, corydlis bungeana 0.3～0.5, birthwort 0.2～0.5, barberry 0.1～0.5, radix bupleuri 0.2～0.4, beta glucan 0.05, yeast 0.02 and manna oligosacchride.
4. thorn seedling immunity Chinese herb feed additive is characterized in that the weight ratio of said component is: 0.04 part of the Radix Astragali 11～15, Radix Codonopsis 8～10, the bark of eucommia 7～9, honeysuckle 6～8, betel nut 5～7, dried orange peel 3～7, rheum officinale 3～6, folium isatidis 3～8, the bark of ash 2～4, Ligusticum wallichii 2～4, Chinese gall 3～7, the root of large-flowered skullcap 2～4, kuh-seng 1～3, garlic 2～5, eupatorium 0.6～0.8, corydlis bungeana 0.3～0.5, birthwort 0.3～0.7, barberry 0.1～0.3, radix bupleuri 0.2～0.4, beta glucan 0.06, yeast 0.03 and manna oligosacchride.
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102370839A (en) * | 2011-11-30 | 2012-03-14 | 刘正殿 | Medicament for treating sea cucumber disease |
| CN103222554A (en) * | 2012-12-24 | 2013-07-31 | 大连海洋大学 | Compound Chinese herbal medicine additive for sea cucumber and preparation method thereof |
| CN103609913B (en) * | 2013-12-04 | 2015-09-09 | 山东省海洋生物研究院 | A kind of Stichopus japonicus compound preparation for strengthening Stichopus japonicus immunologic function |
| CN103931893A (en) * | 2014-04-23 | 2014-07-23 | 大连金海湾养殖科技有限公司 | Traditional Chinese medicinal herbal immunological enhancement additive for aquatic products, preparation method and application thereof |
| CN104585251A (en) * | 2015-02-13 | 2015-05-06 | 中国水产科学研究院黄海水产研究所 | Traditional Chinese herbal medicine composition for killing ciona intestinalis in seedling breeding stage of stichopus japonicus |
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