CN104585251A - Traditional Chinese herbal medicine composition for killing ciona intestinalis in seedling breeding stage of stichopus japonicus - Google Patents
Traditional Chinese herbal medicine composition for killing ciona intestinalis in seedling breeding stage of stichopus japonicus Download PDFInfo
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- CN104585251A CN104585251A CN201510079031.5A CN201510079031A CN104585251A CN 104585251 A CN104585251 A CN 104585251A CN 201510079031 A CN201510079031 A CN 201510079031A CN 104585251 A CN104585251 A CN 104585251A
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Abstract
The invention discloses a traditional Chinese herbal medicine composition for killing ciona intestinalis in seedling breeding stage of stichopus japonicas and belongs to the field of aquaculture drugs. The composition comprises the following components: tea saponin, pumpkin seeds, radix scutellariae and rheum officinale which are added according to the mass ratio of 7 to 1 to 1 to 1, wherein the size of the crushed particles of the composition is 200-300 meshes; the application manner of the composition is medicine bath; the medicine bath time is 24 hours; the medicine dosage is 10mg/L-15mg/L. When the traditional Chinese herbal medicine composition is applied to a breeding pool of stichopus japonicas, the high effect of effectively killing ciona intestinalis can be exerted; the harm of the ciona intestinalis can be greatly reduced; the productivity of the seedling breeding stage of the stichopus japonicas can be obviously increased; the important effects of reducing the chemicals and relieving manual labor intensity can be exerted.
Description
Technical field
The invention belongs to aquaculture drug world, relating to a kind of herbal composite for killing except stichopus japonicus nursery stage Ciona particularly.
Background technology
Enter 21 century, apostichopus japonicus culture is coastal flourish in northern China, and especially Shandong and Liaoning two province, suddenly become one of important new industry of Northern Coastal Region of China aquaculture.Because apostichopus japonicus culture scale is expanded rapidly, the supporting technologies such as disease control relatively lag behind, and add the operation lack of standardization in production process, and some harmful organisms invades in cultivating system, has had a strong impact on the normal growth of stichopus japonicus.Harmful organisms main in apostichopus japonicus culture system has Ciona, copepoda, skeleton shrimp and bristle algae etc.Wherein, Ciona endangers seriously in seedling system, and effectively controls the way broken out of Ciona, is a great problem of puzzlement stichopus japonicus nursery.
Ciona found macroscopic individuality at the beginning of 6 months, and August peaks, and quantity is up to 1062/m
2.Ciona is attached on seedling adhesive plate in a large number, can fight for living space and bait with stichopus japonicus, and consume dissolved oxygen, make stichopus japonicus poor growth, seed output reduces greatly.Meanwhile, Ciona drains a large amount of ammonia nitrogens in stichopus japonicus nursery pond, causes serious harm to stichopus japonicus nursery.
Before the present invention is formed, to focus mostly at ecological factor in its impact and its respiratory metabolism about the report of culturing stichopus japonicus " Ciona ", and less to its prevention and control research report, there is no effective ways or medicine kills except Ciona.In the face of the Ciona that breaks out in a large number, raiser's majority adopts pond, changes plate, peel manually from method remove, and manual removal workload is large, consumes a large amount of human and material resources resource, also cannot reach the effective prevention and control object to Ascidian in a short time.Some culturist carries out sand filtration, Ultraviolet radiation or chemicals sterilization when intaking, attempt to deal with problem from water source.But because stichopus japonicus seedling raise period is oversize, stichopus japonicus nursery operation is lack of standardization, and the probability that Ciona occurs in nursery pond is high, have impact on the production effect of stichopus japonicus nursery significantly.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of herbal composite killed except stichopus japonicus nursery stage Ciona.Medicine of the present invention can kill the Ciona in stichopus japonicus seedling system, and protection juvenile stichopus normal growth, significantly improves survival rate, promotes the productivity effect of nursery, and on the growth of juvenile stichopus, survive without any impact.
The technical solution adopted in the present invention is:
Kill the herbal composite except stichopus japonicus nursery stage Ciona, the composition in composition and mass ratio are: Tea Saponin: pumpkin seeds: the root of large-flowered skullcap: rheum officinale=7:1:1:1;
Further, the grinding particle size of described composition is 200 ~ 300 orders.
Kill the herbal composite except stichopus japonicus nursery stage Ciona, in composition wherein and 1000g composition, the quality of each composition is Tea Saponin 700g, pumpkin seeds 100g, root of large-flowered skullcap 100g, rheum officinale 100g; Grinding particle size is 200 ~ 300 orders, mixes and forms.
Further, the occupation mode of described composition is dipping, and the dipping time is 24h, and drug dose is 10mg/L ~ 15mg/L.Water is changed after disposable medication dipping 24h.After medication the 3rd day fall pond, within the 7th day, change plate, effectively can remove the Ciona in seedling system.
The present invention and peel manually from compared with beneficial effect:
1, the present invention uses special Chinese herbal medicine to kill except Ciona, disposable dipping process, has cheap, economical and efficient, the feature such as easy to use.
2, apply Chinese herbal medicine pollution-free to ecotope, medicine is not produced to stichopus japonicus residual, green safety, to have no side effect, ensure the quality safety of aquatic products.
3, the present inventor takes the lead in having carried out the Chinese herbal medicine killed except Ciona and screens, and establishes the method for killing except Ciona in stichopus japonicus seedling system.Confirmed by production practices: proper use of special Chinese herbal medicine in stichopus japonicus nursery pond, can reach the good result of effectively killing except Ciona, greatly reduce the harm of glass Ascidian, significantly improve the production capacity of stichopus japonicus nursery stage.This blindly uses disinfectant to minimizing, minimizing hand labour intensity all has important practical significance.
Embodiment
Present inventor has performed the research of the special-purpose medicaments killed except Ciona, have developed a kind of efficient, nontoxic special compound Chinese herb, and clinical demonstration has been carried out to its implementation result and using method.Result shows: adopt this special Chinese herb compound to coordinate certain aquaculture model, can reach the good result of killing except Ciona.
Describe technology contents of the present invention in detail with embodiment below, but protection scope of the present invention is not by any pro forma restriction of embodiment.
Embodiment 1
One, the enforcement of medicine is tested and kills except effect
1 test nursery frame: the nursery frame of test is collected in Jiangnan, Qingdao City stichopus japonicus nursery at (comprising the Ciona of attachment), is positioned in the PVC tank filling 150L seawater and supports temporarily, killing except test for medicine after 48h.
2 test specifications and density: in nursery frame, on seedling adhesive plate, the long scope of the individual body of Ciona is 1 ~ 10mm, average attachment density is 800 ~ 1000 Ge/㎡; The long scope of children's individual body of ginseng is 20 ~ 25mm, and average attachment density is 150 ~ 180 Tou/㎡.
3 trial drugs: the present composition, in composition wherein and 1000g composition, the quality of each composition is Tea Saponin 700g, pumpkin seeds 100g, root of large-flowered skullcap 100g, rheum officinale 100g, and grinding particle size is 200 ~ 300 orders, mixes and forms.
4 test procedures: drug concentration is set to 0,5,6,8,10,15,20 and 25mg/L respectively, each concentration group arrange two parallel.In experimental trough, Chinese herb compound medicine is added, experimental session water temperature 22 ~ 24 DEG C, salinity 30, continuous charge, 2 stichopus japonicus powder bait of throwing something and feeding every day by the drug concentration set, change water 1/2 and inhale the end 1 time, this test is respectively at 1h, 3h, 6h, 9h, 12h, 18h, 24h observed and recorded result.Recovering test is transferred in fresh seawater for the Ascidian that come off from seedling adhesive plate by medication 24h or stichopus japonicus seedling, observes the recovery situation being placed in Ascidian or stichopus japonicus seedling in fresh seawater 24h.
5 result of the tests: as shown in Table 1, when Chinese herb medicine concentration reaches 10mg/L, drug effect 24h detects the death of all Ascidians and proceeds in fresh seawater and cannot recover, and has 100% lethal effect to Ciona.Chinese herbal medicine also will ensure the normal growth not affecting stichopus japonicus while killing except Ciona.From table 1, table 2 is learnt, when drug concentration is less than 15mg/L, medicine is joined without physiological effect to children, and seedling is attached on adherance, and corn stretches, and motion freely, is ingested normal; Drug concentration is 15mg/L, when drug effect reaches 24h, there is slight drug response in seedling, about there is the seedling of 10% to show as quil and have contraction, but the seedling shunk by quil moves in fresh seawater, seedling can recover gradually, and after 6h, whole sea cucumbers all recovers normally (see table 2); When drug concentration is more than 15mg/L, part seedling occurs that adhesive force reduces, feeding rate decreased, and part is with the dirty reaction of row, and seedling recovers to need the long period, and even part can not be recovered.
Comprehensive analysis finds: grinding particle size is that the effect of 200 ~ 300 object Chinese herbal medicines to different size Ciona is obvious.Chinese herb medicine concentration is when 10mg/L ~ 15mg/L, and 24 hours dipping time, take into account and kill except Ciona and the effect not affecting stichopus japonicus normal growth two aspect, suitable for producing is promoted the use of.
Table 1 variable concentrations Chinese herb compound kills except test effect Ascidian
Note: B represents Ciona, C represents stichopus japonicus; + expression Ascidian/stichopus japonicus all survives, and 0 represents Ascidian Mortality/stichopus japonicus light contraction ,-represent that Ascidian death/stichopus japonicus contraction is serious and many with the dirty phenomenon of row
The restoration result of children's ginseng after the process of table 2 Chinese herbal medicine
Two, compound Chinese herb produces the experiment of removing Ascidian effect stichopus japonicus seedling stage
Kill except test obtains by Ascidian: medication optimum concentration is 10mg/L ~ 15mg/L, under this concentration, after dipping 24h, Ascidian can come off from adherance completely and Ascidian be had to the lethal effect of 100%, and has no side effect to sea cucumber seed.The present inventor in Qingdao, two, Yantai stichopus japonicus nursery carries out industrial experimentation, duration of test, apostichopus japonicus Selenka feed of normally throwing something and feeding respectively.In the stichopus japonicus nursery of Yantai, when using 10mg/L dosage, during drug effect 3d, large gauge Ascidian (> 0.8cm) substantially all comes off, although small dimension Ascidian does not come off, physical rupture, individual without survival Ascidian on adherance; When using 15mg/L dosage, the same day, Ascidian outlet pipe closed, and health tightens.During drug effect 24h, have large gauge Ascidian seed to come off, drug effect 2d visceral mass is in yellow muddy, and artificial stimulation is reactionless, 80% Ascidian physical rupture, detects individual without survival Ascidian.Also the dosage of 10mg/L and 15mg/L is used respectively at Qingdao nursery.At dipping 1d, all occur that large gauge Ascidian comes off under two groups of concentration, small dimension Ascidian is tightened, little Ascidian physical rupture after dipping 2d, and seedling adhesive plate Examined shows on adherance all individual without survival Ascidian.
Above-mentioned two nurseries, fall pond after all adopting dipping 3d.Now, most larger Ascidian individuality is shed at the bottom of pond, but sea cucumbers majority is attached on seedling adhesive plate, easily makes sea cucumbers be separated with Ciona by falling pond.Comparatively speaking, although the little individual Ascidian on seedling adhesive plate is all dead, Ascidian histolysis intersperses among in the water of pond approximately needs about 4d.Therefore, after dipping, 7d carries out changing plate.Like this, fall by 3d the mode that pond, 7d change plate and just completely sea cucumbers is separated with Ciona, shift out outside nursery pond.Kill except after Ciona, substantially improve the growing environment of children's ginseng, accelerate the growth rate of children's ginseng, improve survival rate and output; Its sea cucumbers be separated stretches good, strong adhesion, it is normal to ingest, and holistic health is good.In addition, this compound Chinese herb using method is easy, with low cost, and substitute peel manually from Ciona, every nursery can save 20 ~ 30 labours, is applicable to wide popularization and application.
Claims (4)
1. kill the herbal composite except stichopus japonicus nursery stage Ciona, the composition in composition and mass ratio are: Tea Saponin: pumpkin seeds: the root of large-flowered skullcap: rheum officinale=7:1:1:1.
2. composition according to claim 1, is characterized in that the grinding particle size of described composition is 200 ~ 300 orders.
3. composition according to claim 1, the quality that it is characterized in that each composition in composition in described composition and 1000g composition is Tea Saponin 700g, pumpkin seeds 100g, root of large-flowered skullcap 100g, rheum officinale 100g, grinding particle size is 200 ~ 300 orders, mixes and forms.
4. be dipping according to the occupation mode of the composition of claim 1-3 described in any one, the dipping time is 24h, and drug dose is 10mg/L ~ 15mg/L.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102058020A (en) * | 2009-11-11 | 2011-05-18 | 大连水产学院 | Chinese medicinal herb additive for improving immunity of juvenile sea cucumber |
| CN102870709A (en) * | 2011-07-14 | 2013-01-16 | 烟台大学 | Artificial optimized culture method for sea squirt, microalgae and stichopus japonicus |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102058020A (en) * | 2009-11-11 | 2011-05-18 | 大连水产学院 | Chinese medicinal herb additive for improving immunity of juvenile sea cucumber |
| CN102870709A (en) * | 2011-07-14 | 2013-01-16 | 烟台大学 | Artificial optimized culture method for sea squirt, microalgae and stichopus japonicus |
Non-Patent Citations (3)
| Title |
|---|
| 吴湛辉: "北方地区刺参育苗生产的几项实用技术", 《河北渔业》 * |
| 汪建国: "我国渔药研究与应用综述（１）", 《渔业致富指南》 * |
| 王印庚 等: "一种中草药复方对刺参苗期玻璃海鞘的杀除效果", 《水产科技情报》 * |
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