CN102017850B - Oil tea double embryo stem anvil grafting nutritive cup breeding method - Google Patents
Oil tea double embryo stem anvil grafting nutritive cup breeding method Download PDFInfo
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- CN102017850B CN102017850B CN 200910044349 CN200910044349A CN102017850B CN 102017850 B CN102017850 B CN 102017850B CN 200910044349 CN200910044349 CN 200910044349 CN 200910044349 A CN200910044349 A CN 200910044349A CN 102017850 B CN102017850 B CN 102017850B
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- 238000009395 breeding Methods 0.000 title claims abstract description 16
- 210000001161 mammalian embryo Anatomy 0.000 title claims abstract description 12
- 230000000050 nutritive effect Effects 0.000 title claims abstract description 11
- 241001122767 Theaceae Species 0.000 title abstract description 18
- 230000001488 breeding effect Effects 0.000 claims abstract description 14
- 235000016709 nutrition Nutrition 0.000 claims abstract description 12
- 230000035764 nutrition Effects 0.000 claims abstract description 12
- 238000000034 method Methods 0.000 claims abstract description 6
- 239000002689 soil Substances 0.000 claims abstract description 6
- 239000003921 oil Substances 0.000 claims description 15
- 239000004576 sand Substances 0.000 claims description 8
- 239000010495 camellia oil Substances 0.000 claims description 5
- 244000147058 Derris elliptica Species 0.000 claims description 4
- 241000196324 Embryophyta Species 0.000 claims description 4
- 241000238631 Hexapoda Species 0.000 claims description 4
- 239000005030 aluminium foil Substances 0.000 claims description 4
- 201000010099 disease Diseases 0.000 claims description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 4
- 235000013399 edible fruits Nutrition 0.000 claims description 4
- 230000008030 elimination Effects 0.000 claims description 3
- 238000003379 elimination reaction Methods 0.000 claims description 3
- 239000012535 impurity Substances 0.000 claims description 3
- 238000003780 insertion Methods 0.000 claims description 3
- 230000037431 insertion Effects 0.000 claims description 3
- 239000011159 matrix material Substances 0.000 claims description 3
- 235000015097 nutrients Nutrition 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 235000009024 Ceanothus sanguineus Nutrition 0.000 claims description 2
- 240000003553 Leptospermum scoparium Species 0.000 claims description 2
- 235000015459 Lycium barbarum Nutrition 0.000 claims description 2
- 108010016634 Seed Storage Proteins Proteins 0.000 claims description 2
- 230000032696 parturition Effects 0.000 claims description 2
- 230000001954 sterilising effect Effects 0.000 claims description 2
- 238000004659 sterilization and disinfection Methods 0.000 claims description 2
- 244000269722 Thea sinensis Species 0.000 claims 1
- 230000004083 survival effect Effects 0.000 abstract description 9
- 238000010521 absorption reaction Methods 0.000 abstract description 2
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 abstract 1
- 229910052782 aluminium Inorganic materials 0.000 abstract 1
- 239000011888 foil Substances 0.000 abstract 1
- 230000035784 germination Effects 0.000 abstract 1
- 238000004804 winding Methods 0.000 abstract 1
- 235000019198 oils Nutrition 0.000 description 13
- 240000001548 Camellia japonica Species 0.000 description 5
- 235000018597 common camellia Nutrition 0.000 description 5
- 235000013339 cereals Nutrition 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 241000526900 Camellia oleifera Species 0.000 description 2
- 239000005842 Thiophanate-methyl Substances 0.000 description 2
- HYBBIBNJHNGZAN-UHFFFAOYSA-N furfural Chemical compound O=CC1=CC=CO1 HYBBIBNJHNGZAN-UHFFFAOYSA-N 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- QGHREAKMXXNCOA-UHFFFAOYSA-N thiophanate-methyl Chemical group COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC QGHREAKMXXNCOA-UHFFFAOYSA-N 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 239000000645 desinfectant Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000008157 edible vegetable oil Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000007937 lozenge Substances 0.000 description 1
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 description 1
- 239000004006 olive oil Substances 0.000 description 1
- 235000008390 olive oil Nutrition 0.000 description 1
- 239000002420 orchard Substances 0.000 description 1
- 238000005498 polishing Methods 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 229930182490 saponin Natural products 0.000 description 1
- 150000007949 saponins Chemical class 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 229920001864 tannin Polymers 0.000 description 1
- 239000001648 tannin Substances 0.000 description 1
- 235000018553 tannin Nutrition 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 description 1
- 229960002675 xylitol Drugs 0.000 description 1
- 235000010447 xylitol Nutrition 0.000 description 1
- 239000000811 xylitol Substances 0.000 description 1
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Abstract
Provided is a an oil tea double embryo stem anvil grafting nutritive cup breeding method, comprising seed collecting, seed storing, accelerating germination, scion selecting, double embryo stem grafting and nutritive cup breeding. The method is characterized by the double embryo stem grafting, which comprises the following steps of cutting two lower surfaces of scions into horse ear-shaped surfaces so as to make the lower part of the scions thin wedge-shaped, in the meantime taking two cut-off embryo stems with seeds and cutting the upper end of each stem to form a horse ear-shaped surface, juxtaposing the horse ear-shaped surfaces of the two embryo stems face-to-face, inserting the scions between the two embryo stems, binding tightly with aluminum foils or winding tightly with grafting films, directly planting grafted seedlings into a black plastic nutritive cup, and planting the seedlings in the nutritive cup into a plantation site together with the soil when the grafted seedlings grow to more than 10 centimeters tall. The present invention provides a wider nutrition source for scion seeds and an enlarged absorption area for roots, significantly raising the survival rate of the seedlings. In addition, the use of the nutritive cup further enables the seedlings to grow in a more rapid and better manner, greatly raising the survival rate of the grafted seedlings.
Description
[technical field] the present invention relates to camellia seedling, i.e. a kind of oil tea double embryo stem anvil grafting nutritive cup breeding.
[background technology] oil tea is world-renowned four large woody oil tree species, and tea oil is praised title " east olive oil ".Tea oil can be refined into high-grade environmental protection edible oil, and high-class healthy product and cosmetics are produced in deep processing; Withered Tea Saponin, polishing powder and the tea dregs of rice produced of tea; The tea shell can be used for making furfural, xylitol, tannin extract, active carbon and medium.Be that oil tea is precious from head to foot.
In recent years, for national grain and oil safety, the Party Central Committee, State Council and Party committees at all levels, government have all mentioned important agenda to camellia oleiferaindustry.At present, every oil tea normal region is unprecedentedly surging to the enthusiasm of oil tea development, yet the supply of oil tea breeding seedling has extremely lacked the bottleneck into the fast development of serious restriction camellia oleiferaindustry, for addressing this problem as early as possible, all wideling popularize so-called single bud anvil graft technology at present, though this technology makes progress current comparing with other conventional vegetative propagation technique, survival rate, planting percent are still undesirable, and be also very incompatible with the demand of camellia oleiferaindustry development.
[summary of the invention] purpose of the present invention overcomes existing problem in the present oil tea grafting method exactly, and a kind of oil tea double embryo stem anvil grafting nutritive cup breeding that provides.
This oil tea double embryo stem anvil grafting nutritive cup breeding comprises seed collection, seed storage, presprouting of seeds, chooses scion, two blastema grafting and container cup grow seedlings, and it is characterized in that concrete steps are as follows:
One, seed collection: gather in the fall ripe shinny, grain is full greatly, without the Property of Tea oil Tree Fruit of damage by disease and insect;
Two, seed stores: except room temperature commonly used is done husky storage method, take 0 ℃ of refrigeration as good;
Three, presprouting of seeds: March then the seed that stores is taken out, with wet husky vernalization, in 5 months, the anvil grafting of can breaking during blastema length to 4～6cm in the last ten-days period;
Four, scion is chosen: choose in the breeding oil tea tree crown periphery, the top axillalry bud is full, blade is without the branch of giving birth to then semi-lignified of damage by disease and insect;
Five, two blastema graftings: one section of 3～5cm is cut in the scion that will adopt back, keep a leaf and a bud, the two sides, bottom is whittled into Ma Ermian, the angle of horse ear face and vertical plane is 20 °～30 °, long 1～the 1.5cm of bevel, make the scion bottom become thin wedge shape, simultaneously, with blastema together with seed from damp sand, take out remove silt after, cut off at blastema section 3～4cm place, get the disconnected blastema with seed of 2 undercuts, respectively cut in the top a horse ear shape face, the angle of horse ear face and vertical plane is 20 °～30 °, and the long 1～1.5cm of bevel is in juxtaposition the horse ear shape face of 2 blastemas in opposite directions, again in the middle of two blastemas of scion insertion, each horse ear is clutched with the aluminium foil colligation, or is twined with the grafting film in the face of neat;
Six, nutrition cup is grown seedlings: in the black plastic nutrition cup with nutrient matrix of the direct implanted diameter 8 * 9cm of the seedling that grafting is good or 9 * 9cm, water permeable in conjunction with sterilization, remove after 25 days and sprout impurity elimination, seedling to be grafted grows to 10cm when above, just seedling in the nutrition cup can be connected soil and plant together planting site, but go up a hill as good when being better than most the early spring in next year.
So characteristics of the present invention with the grafting of a fringe bar on the two blastema stocks that are not unearthed, both increased the nutrient source of supplying with ingraftment seed, also enlarged simultaneously the absorption area of root system, greatly improved the survival rate of seedling, can sprout ahead of time again and take out the tip, matrix in the nutrition cup is the Nutrition Soil through scientific matching in addition, make plant strain growth more rapid, healthy and strong, May 27, grafting was measured its survival rate up to more than 95% in then random sampling on August 21 then, height of seedling reaches 11.1cm, the number of blade is up to more than 10, than 6 days in advance and there is under the spraying facility condition the so-called single bud anvil survival rate in grafting on May 21 high by 35%, height of seedling 32%, the number of blade many 30%.In the situation that breeding fringe bar is in short supply at present, the present invention can take full advantage of limited improved variety scion, greatly improves the survival rate of grafting, can be applicable to again quickly breeding grafting seedlings, set up the breeding cutting orchard, obtain ahead of time breeding fringe bar, accelerate the camellia oleiferaindustry process.
[description of drawings] Fig. 1 is the schematic diagram of grafting of the present invention
Among the figure: 1, scion, 2, blastema, 3, aluminium foil.
[embodiment]
The below enumerates an embodiment, and is as follows to the detailed description of the invention:
One, in October, 2007, at changde, hunan province city big dragon station, gather ripe light, full grain large, without the camellia oleifera fruit 10kg of damage by disease and insect;
Two, seed stores, and the camellia oleifera fruit of adopting back is placed on the room ventilation place, allows its season crack, then tea seed is buried storage with clean dry river sand;
Three, presprouting of seeds, is taken out tea seed on March 15th, 2008, with wet husky vernalization, wet sand is used first the thiophanate methyl liquid disinfectant, and the humidity of sand can be pinched agglomerating, loose with hand and namely be fallen apart and be advisable, one deck is husky, one deck seed, interseminal sand thickness 10cm spreads three layers or multilayer altogether, waters one time water in 4～5 days, keep the moistening of sand, May 25, the oil tea blastema grows into 4～6cm, the beginning grafting;
Four, choose scion, on the breeding tea oil tree, choose in the tree crown periphery, the top axillalry bud is full, give birth to then the branch of semi-lignified as scion;
Five, two blastema graftings, in Fig. 1, one section of 4cm is cut in the scion 1 of adopting back, keep a leaf one bud, the two sides, bottom is whittled into Ma Ermian, and the angle of horse ear face and vertical plane is 30 °, the long 1～1.5cm of bevel, make the scion bottom become thin wedge shape, simultaneously, silt is removed in blastema 2 taking-up from wet sand, cut off at 3～4cm place, seed top, get the disconnected blastema of 2 undercuts and respectively cut a horse ear face in the upper end, the angle of horse ear face and vertical plane is 30 °, and the long 1～1.5cm of bevel is in juxtaposition the horse ear face of two blastemas 2 in opposite directions, in the middle of two blastemas of the lozenges of scion 1 insertion, each horse ear is in the face of clutching with aluminium foil 3 colligations together;
Six, nutrition cup is grown seedlings, and the direct implanted diameter 9cm of the seedling that grafting is good is in the nutrition cup of high 10cm, Nutrition Soil is thin loess, in be mixed with 1.5% the fertilizer that becomes thoroughly decomposed, after the grafting the 15th day, spray once with thiophanate methyl liquid again, after 25 days, remove shady impurity elimination, on October 30th, 2008, grafting has grown to 10～11cm, the number of blade reaches more than 13, and is higher by 20% than simple bud stock grafting seedling, the number of blade many 20%.After yongyangmo connected soil plant together on the planting site, the nursery stock growing way is vigorous on the mountain at present, survival rate reaches 96%, than the same year simple bud stock grafting seedling survival rate high 30 percentage points.
Claims (1)
1. oil tea double embryo stem anvil grafting nutritive cup breeding comprises seed collection, seed storage, presprouting of seeds, chooses scion, two blastema grafting and container cup grow seedlings, and it is characterized in that concrete steps are as follows:
One, seed collection: gather in the fall ripe shinny, grain is full greatly, without the Property of Tea oil Tree Fruit of damage by disease and insect;
Two, seed stores: do husky storage method with room temperature;
Three, presprouting of seeds: March then the seed that stores is taken out, with wet husky vernalization, in 5 months, the anvil grafting of can breaking during blastema length to 4～6 ㎝ in the last ten-days period;
Four, scion is chosen: choose in the breeding oil tea tree crown periphery, full, the anosis worm of blade of top axillalry bud
The branch of giving birth to then semi-lignified of evil;
Five, two blastema graftings: one section of 3～5 ㎝ are cut in the scion that will adopt back, keep a leaf and a bud, will
The two sides, bottom is whittled into Ma Ermian, the angle of horse ear face and vertical plane is 20 °～30 °, long 1～1.5 ㎝ of bevel, make the scion bottom become thin wedge shape, simultaneously, with blastema together with seed from damp sand, take out remove silt after, cut off at blastema section 3～4 ㎝ places, get the disconnected blastema with seed of 2 undercuts, respectively cut in the top a horse ear shape face, the angle of horse ear face and vertical plane is 20 °～30 °, and long 1～1.5 ㎝ of bevel is in juxtaposition the horse ear shape face of 2 blastemas in opposite directions, again in the middle of two blastemas of scion insertion, each horse ear is clutched with the aluminium foil colligation, or is twined with the grafting film in the face of neat;
Six, nutrition cup is grown seedlings: in the black plastic nutrition cup with nutrient matrix of direct implanted diameter 8 * 9 ㎝ of the seedling that grafting is good or 9 * 9 ㎝, water permeable in conjunction with sterilization, remove after 25 days and sprout impurity elimination, seedling to be grafted grows to 10 ㎝ when above, seedling in the nutrition cup is connected soil plant together planting site, when the early spring in next year, go up a hill.
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| CN 200910044349 CN102017850B (en) | 2009-09-12 | 2009-09-12 | Oil tea double embryo stem anvil grafting nutritive cup breeding method |
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| CN102017850B true CN102017850B (en) | 2013-01-23 |
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| CN102726217B (en) * | 2012-06-06 | 2015-05-13 | 安徽德昌苗木有限公司 | Container seedling raising method for camellia oleifera bud-stock grafting |
| CN102804997A (en) * | 2012-08-07 | 2012-12-05 | 中国林业科学研究院亚热带林业研究所 | Grafting and seedling-cultivating method for Theaceae plant |
| CN105794497B (en) * | 2014-12-30 | 2019-01-15 | 李延梅 | A kind of engrafting method of olive |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1903003A (en) * | 2006-08-10 | 2007-01-31 | 刘成忠 | Method for grafting by using two root stocks |
| CN101124877A (en) * | 2007-07-17 | 2008-02-20 | 攸县林业局 | Growing seedlings method for oil-tea sprout with grafting container cup |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH1042695A (en) * | 1996-08-01 | 1998-02-17 | Iseki & Co Ltd | Device for temporarily planting grafted seedling |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1903003A (en) * | 2006-08-10 | 2007-01-31 | 刘成忠 | Method for grafting by using two root stocks |
| CN101124877A (en) * | 2007-07-17 | 2008-02-20 | 攸县林业局 | Growing seedlings method for oil-tea sprout with grafting container cup |
Non-Patent Citations (5)
| Title |
|---|
| JP特开平10－42695A 1998.02.17 |
| 孙倩等.油茶胚轴嫁接时期与培育期施肥方式的研究.《安徽农业科学》.2006,第34卷(第06期), |
| 油茶丰产栽培技术研究;陈鼎源等;《经济林研究》;19961231;第14卷(第S1期);全文 * |
| 油茶胚轴嫁接时期与培育期施肥方式的研究;孙倩等;《安徽农业科学》;20060331;第34卷(第06期);全文 * |
| 陈鼎源等.油茶丰产栽培技术研究.《经济林研究》.1996,第14卷(第S1期), |
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