CN102016018B - 用于生产乙醇酸的固定的微生物腈水解酶的改进 - Google Patents

用于生产乙醇酸的固定的微生物腈水解酶的改进 Download PDF

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CN102016018B
CN102016018B CN2008801241192A CN200880124119A CN102016018B CN 102016018 B CN102016018 B CN 102016018B CN 2008801241192 A CN2008801241192 A CN 2008801241192A CN 200880124119 A CN200880124119 A CN 200880124119A CN 102016018 B CN102016018 B CN 102016018B
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CN102016018A (zh
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R·迪科西莫
A·本-巴萨特
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High Purity Technology Science Co ltd
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EI Du Pont de Nemours and Co
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/06—Enzymes or microbial cells immobilised on or in an organic carrier attached to the carrier via a bridging agent
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN2008801241192A 2007-10-31 2008-10-31 用于生产乙醇酸的固定的微生物腈水解酶的改进 Expired - Fee Related CN102016018B (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US11/930,744 2007-10-31
US11/930744 2007-10-31
US11/930,744 US7741088B2 (en) 2007-10-31 2007-10-31 Immobilized microbial nitrilase for production of glycolic acid
PCT/US2008/081952 WO2009059104A1 (en) 2007-10-31 2008-10-31 Improvement in immobilized microbial nitrilase for production of glycolic acid

Publications (2)

Publication Number Publication Date
CN102016018A CN102016018A (zh) 2011-04-13
CN102016018B true CN102016018B (zh) 2013-09-25

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US (2) US7741088B2 (enExample)
EP (1) EP2215226B9 (enExample)
JP (1) JP5583586B2 (enExample)
CN (1) CN102016018B (enExample)
DK (1) DK2215226T3 (enExample)
ES (1) ES2562791T3 (enExample)
PL (1) PL2215226T3 (enExample)
WO (1) WO2009059104A1 (enExample)

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EP2480661A1 (en) * 2009-09-24 2012-08-01 UCB Pharma, S.A. Bacterial host strain
CA2796158C (en) 2010-04-14 2022-06-14 Strategic Enzyme Applications, Inc. Process for producing phosphinothricin employing nitrilases
WO2012003336A2 (en) 2010-06-30 2012-01-05 Codexis, Inc. Chemically modified carbonic anhydrases useful in carbon capture systems
WO2012048865A2 (de) * 2010-10-12 2012-04-19 C-Lecta Gmbh Nitrilasen
EP3354742A1 (en) 2017-01-26 2018-08-01 Metabolic Explorer Methods and microorganisms for the production of glycolic acid and/or glyoxylic acid
CN108486088B (zh) 2018-02-14 2021-02-02 浙江工业大学 腈水解酶突变体及其应用
CN113195727A (zh) * 2018-10-18 2021-07-30 巴斯夫欧洲公司 用于产生丙烯酸或其盐的方法
CN112063607B (zh) * 2020-10-09 2021-12-07 浙江工业大学 一种腈水解酶突变体及其在催化合成2-氯烟酸中的应用
CN119570649A (zh) * 2024-07-12 2025-03-07 北京衍微科技有限公司 含红球菌发酵液的复合催化剂及其用途

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001004278A1 (en) * 1999-07-12 2001-01-18 E.I. Du Pont De Nemours And Company Method for stabilizing nitrilase activity and preserving microbial cells
CN1409756A (zh) * 1999-11-12 2003-04-09 三菱丽阳株式会社 灭活的微生物细胞
WO2006069114A2 (en) * 2004-12-22 2006-06-29 E.I. Dupont De Nemours And Company Process for producing glycolic acid from formaldehyde and hydrogen cyanide
WO2007036235A1 (en) * 2005-09-30 2007-04-05 Novozymes A/S Immobilization of enzymes

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DK146942C (da) 1978-04-19 1984-07-30 Novo Industri As Fremgangsmaade til fremstilling af et immobiliseret enzymprodukt
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JPS6156086A (ja) 1984-08-27 1986-03-20 Asahi Chem Ind Co Ltd α−オキシ酸およびその塩の微生物学的製造法
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DE69131217T2 (de) * 1990-03-30 1999-09-23 Mitsubishi Rayon Co., Ltd. Verfahren zur Herstellung von R(-)Mandelsäure und deren Derivaten
JP2974737B2 (ja) * 1990-08-16 1999-11-10 三菱レイヨン株式会社 光学活性乳酸の製造法
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JP2676568B2 (ja) * 1991-06-26 1997-11-17 日東化学工業株式会社 Ｒ（−）−マンデル酸およびその誘導体の製造法
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JP2720140B2 (ja) 1993-02-03 1998-02-25 日東化学工業株式会社 フェニル基を有する光学活性α−ヒドロキシカルボン酸の製造法
JP3354688B2 (ja) 1994-01-28 2002-12-09 三菱レイヨン株式会社 微生物によるα−ヒドロキシ酸またはα−ヒドロキシアミドの製造法
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US6416980B1 (en) 2001-02-23 2002-07-09 E. I. Du Pont De Nemours & Company Method for producing glycolic acid from glycolonitrile using nitrilase
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US7148051B2 (en) 2004-08-16 2006-12-12 E. I. Du Pont De Nemours And Company Production of 3-hydroxycarboxylic acid using nitrilase
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001004278A1 (en) * 1999-07-12 2001-01-18 E.I. Du Pont De Nemours And Company Method for stabilizing nitrilase activity and preserving microbial cells
CN1409756A (zh) * 1999-11-12 2003-04-09 三菱丽阳株式会社 灭活的微生物细胞
WO2006069114A2 (en) * 2004-12-22 2006-06-29 E.I. Dupont De Nemours And Company Process for producing glycolic acid from formaldehyde and hydrogen cyanide
WO2007036235A1 (en) * 2005-09-30 2007-04-05 Novozymes A/S Immobilization of enzymes

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PL2215226T3 (pl) 2016-06-30
JP5583586B2 (ja) 2014-09-03
US20090111148A1 (en) 2009-04-30
ES2562791T3 (es) 2016-03-08
DK2215226T3 (en) 2016-03-07
JP2011501970A (ja) 2011-01-20
EP2215226A1 (en) 2010-08-11
WO2009059104A1 (en) 2009-05-07
US7741088B2 (en) 2010-06-22
US7867748B2 (en) 2011-01-11
CN102016018A (zh) 2011-04-13
EP2215226B9 (en) 2016-03-02
ES2562791T9 (es) 2016-04-18
EP2215226B1 (en) 2015-12-02
US20100136656A1 (en) 2010-06-03

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