CN101985637A - 一种微生物油脂的提取方法 - Google Patents
一种微生物油脂的提取方法 Download PDFInfo
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- CN101985637A CN101985637A CN 201010527702 CN201010527702A CN101985637A CN 101985637 A CN101985637 A CN 101985637A CN 201010527702 CN201010527702 CN 201010527702 CN 201010527702 A CN201010527702 A CN 201010527702A CN 101985637 A CN101985637 A CN 101985637A
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- 230000000813 microbial effect Effects 0.000 title claims abstract description 26
- 238000000034 method Methods 0.000 title claims abstract description 25
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- 238000000855 fermentation Methods 0.000 claims abstract description 16
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- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 claims description 10
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 9
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 8
- IMNFDUFMRHMDMM-UHFFFAOYSA-N N-Heptane Chemical compound CCCCCCC IMNFDUFMRHMDMM-UHFFFAOYSA-N 0.000 claims description 8
- 239000000839 emulsion Substances 0.000 claims description 8
- 230000001580 bacterial effect Effects 0.000 claims description 7
- 238000011081 inoculation Methods 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical group CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 6
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 6
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 6
- 239000003960 organic solvent Substances 0.000 claims description 6
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 4
- 229910017053 inorganic salt Inorganic materials 0.000 claims description 4
- 235000015097 nutrients Nutrition 0.000 claims description 4
- 241001337994 Cryptococcus <scale insect> Species 0.000 claims description 3
- 241000907999 Mortierella alpina Species 0.000 claims description 3
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 claims description 3
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 3
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 2
- 239000001110 calcium chloride Substances 0.000 claims description 2
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 2
- 238000004821 distillation Methods 0.000 claims description 2
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- 230000002349 favourable effect Effects 0.000 abstract 1
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- 238000005516 engineering process Methods 0.000 description 7
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- 235000020777 polyunsaturated fatty acids Nutrition 0.000 description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 5
- 238000000658 coextraction Methods 0.000 description 5
- 239000008103 glucose Substances 0.000 description 5
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- DVSZKTAMJJTWFG-UHFFFAOYSA-N docosa-2,4,6,8,10,12-hexaenoic acid Chemical compound CCCCCCCCCC=CC=CC=CC=CC=CC=CC(O)=O DVSZKTAMJJTWFG-UHFFFAOYSA-N 0.000 description 4
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- 239000011780 sodium chloride Substances 0.000 description 3
- 108010046845 tryptones Proteins 0.000 description 3
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 239000003708 ampul Substances 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
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- 206010053759 Growth retardation Diseases 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 208000006981 Skin Abnormalities Diseases 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
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- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
- 229910000397 disodium phosphate Inorganic materials 0.000 description 1
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- 238000005265 energy consumption Methods 0.000 description 1
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- 231100000001 growth retardation Toxicity 0.000 description 1
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- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
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- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
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Cited By (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102533879A (zh) * | 2010-12-17 | 2012-07-04 | 中国科学院大连化学物理研究所 | 一种微生物油脂提取方法 |
CN102925280A (zh) * | 2011-08-10 | 2013-02-13 | 嘉必优生物工程(湖北)有限公司 | 一种微生物油脂的提取及精炼方法 |
CN103589503A (zh) * | 2012-08-13 | 2014-02-19 | 丰益(上海)生物技术研发中心有限公司 | 一种高效提取微生物油脂的方法 |
CN103849458A (zh) * | 2014-03-21 | 2014-06-11 | 清华大学 | 微藻油脂的提取方法 |
CN103882071A (zh) * | 2014-03-14 | 2014-06-25 | 嘉必优生物工程(武汉)有限公司 | 一种微生物油及其制备方法 |
CN104059773A (zh) * | 2014-07-02 | 2014-09-24 | 华南理工大学 | 一种湿法提取纯化微藻藻油的方法 |
CN104371926A (zh) * | 2013-08-15 | 2015-02-25 | 中国石油化工股份有限公司 | 一种处理微藻培养液的方法 |
CN105219515A (zh) * | 2014-06-18 | 2016-01-06 | 中国石油化工股份有限公司 | 一种含油微藻的破壁及利用皂化反应辅助提取油脂的方法 |
CN105296137A (zh) * | 2015-10-15 | 2016-02-03 | 中国科学院水生生物研究所 | 一种生物酶催化破壁提取微藻油脂的方法 |
CN105669258A (zh) * | 2016-01-06 | 2016-06-15 | 济南开发区星火科学技术研究院 | 一种产油微生物发酵培养液的资源化利用方法 |
CN105738185A (zh) * | 2016-01-28 | 2016-07-06 | 润科生物工程(福建)有限公司 | 一种萃取装置及其在萃取、油脂提取和测定微生物细胞油脂含量中的应用 |
US9738851B2 (en) | 2000-01-19 | 2017-08-22 | Dsm Ip Assets B.V. | Solventless extraction process |
US10342772B2 (en) | 2013-12-20 | 2019-07-09 | Dsm Ip Assets B.V. | Processes for obtaining microbial oil from microbial cells |
US10364207B2 (en) | 2013-12-20 | 2019-07-30 | Dsm Ip Assets B.V. | Processes for obtaining microbial oil from microbial cells |
US10392578B2 (en) | 2010-06-01 | 2019-08-27 | Dsm Ip Assets B.V. | Extraction of lipid from cells and products therefrom |
US10472316B2 (en) | 2013-12-20 | 2019-11-12 | Dsm Ip Assets B.V. | Processes for obtaining microbial oil from microbial cells |
CN112608851A (zh) * | 2020-12-30 | 2021-04-06 | 润科生物工程(福建)有限公司 | 一种提取高山被孢霉所含油脂的方法 |
US11124736B2 (en) | 2013-12-20 | 2021-09-21 | Dsm Ip Assets B.V. | Processes for obtaining microbial oil from microbial cells |
CN115322815A (zh) * | 2022-07-18 | 2022-11-11 | 中润油新能源股份有限公司 | 一种生物基甲醇汽油及其制备方法 |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105505555B (zh) * | 2016-01-28 | 2020-01-03 | 润科生物工程(福建)有限公司 | 一种从含有产油微生物细胞的发酵液中提取油脂的方法 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN101307341A (zh) * | 2008-05-29 | 2008-11-19 | 武汉友芝友保健乳品有限公司 | 生物酶法破壁用于二十二碳六烯酸油脂生产方法 |
CN101323865A (zh) * | 2008-08-07 | 2008-12-17 | 山东省科学院能源研究所 | 微生物油脂分离提取方法 |
CN101824440A (zh) * | 2009-03-04 | 2010-09-08 | 中国科学院大连化学物理研究所 | 一种微生物油脂的分离方法 |
-
2010
- 2010-11-02 CN CN201010527702.7A patent/CN101985637B/zh active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN101307341A (zh) * | 2008-05-29 | 2008-11-19 | 武汉友芝友保健乳品有限公司 | 生物酶法破壁用于二十二碳六烯酸油脂生产方法 |
CN101323865A (zh) * | 2008-08-07 | 2008-12-17 | 山东省科学院能源研究所 | 微生物油脂分离提取方法 |
CN101824440A (zh) * | 2009-03-04 | 2010-09-08 | 中国科学院大连化学物理研究所 | 一种微生物油脂的分离方法 |
Non-Patent Citations (1)
Title |
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《粮食与油脂》 20081231 相光明 等 产油微生物研究及应用 7-11 , 第6期 * |
Cited By (24)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9738851B2 (en) | 2000-01-19 | 2017-08-22 | Dsm Ip Assets B.V. | Solventless extraction process |
US10392578B2 (en) | 2010-06-01 | 2019-08-27 | Dsm Ip Assets B.V. | Extraction of lipid from cells and products therefrom |
CN102533879A (zh) * | 2010-12-17 | 2012-07-04 | 中国科学院大连化学物理研究所 | 一种微生物油脂提取方法 |
CN102533879B (zh) * | 2010-12-17 | 2013-12-04 | 中国科学院大连化学物理研究所 | 一种微生物油脂提取方法 |
CN102925280A (zh) * | 2011-08-10 | 2013-02-13 | 嘉必优生物工程(湖北)有限公司 | 一种微生物油脂的提取及精炼方法 |
CN103589503B (zh) * | 2012-08-13 | 2015-09-30 | 丰益(上海)生物技术研发中心有限公司 | 一种高效提取微生物油脂的方法 |
CN103589503A (zh) * | 2012-08-13 | 2014-02-19 | 丰益(上海)生物技术研发中心有限公司 | 一种高效提取微生物油脂的方法 |
CN104371926A (zh) * | 2013-08-15 | 2015-02-25 | 中国石油化工股份有限公司 | 一种处理微藻培养液的方法 |
US10364207B2 (en) | 2013-12-20 | 2019-07-30 | Dsm Ip Assets B.V. | Processes for obtaining microbial oil from microbial cells |
US11124736B2 (en) | 2013-12-20 | 2021-09-21 | Dsm Ip Assets B.V. | Processes for obtaining microbial oil from microbial cells |
US10472316B2 (en) | 2013-12-20 | 2019-11-12 | Dsm Ip Assets B.V. | Processes for obtaining microbial oil from microbial cells |
US10342772B2 (en) | 2013-12-20 | 2019-07-09 | Dsm Ip Assets B.V. | Processes for obtaining microbial oil from microbial cells |
CN103882071A (zh) * | 2014-03-14 | 2014-06-25 | 嘉必优生物工程(武汉)有限公司 | 一种微生物油及其制备方法 |
CN103882071B (zh) * | 2014-03-14 | 2016-10-05 | 嘉必优生物技术(武汉)股份有限公司 | 一种微生物油及其制备方法 |
CN103849458A (zh) * | 2014-03-21 | 2014-06-11 | 清华大学 | 微藻油脂的提取方法 |
CN103849458B (zh) * | 2014-03-21 | 2016-08-10 | 清华大学 | 微藻油脂的提取方法 |
CN105219515A (zh) * | 2014-06-18 | 2016-01-06 | 中国石油化工股份有限公司 | 一种含油微藻的破壁及利用皂化反应辅助提取油脂的方法 |
CN105219515B (zh) * | 2014-06-18 | 2019-01-08 | 中国石油化工股份有限公司 | 一种含油微藻的破壁及利用皂化反应辅助提取油脂的方法 |
CN104059773A (zh) * | 2014-07-02 | 2014-09-24 | 华南理工大学 | 一种湿法提取纯化微藻藻油的方法 |
CN105296137A (zh) * | 2015-10-15 | 2016-02-03 | 中国科学院水生生物研究所 | 一种生物酶催化破壁提取微藻油脂的方法 |
CN105669258A (zh) * | 2016-01-06 | 2016-06-15 | 济南开发区星火科学技术研究院 | 一种产油微生物发酵培养液的资源化利用方法 |
CN105738185A (zh) * | 2016-01-28 | 2016-07-06 | 润科生物工程(福建)有限公司 | 一种萃取装置及其在萃取、油脂提取和测定微生物细胞油脂含量中的应用 |
CN112608851A (zh) * | 2020-12-30 | 2021-04-06 | 润科生物工程(福建)有限公司 | 一种提取高山被孢霉所含油脂的方法 |
CN115322815A (zh) * | 2022-07-18 | 2022-11-11 | 中润油新能源股份有限公司 | 一种生物基甲醇汽油及其制备方法 |
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Address after: 430074 Wuhan Province, East Lake New Technology Development Zone, high tech Avenue, No. 999, No. Patentee after: Limited by Share Ltd Biotechnology (Wuhan) Co., Ltd. Address before: 430073 Hubei city of Wuhan province Optics Valley Road 1 International Business Center Juxian building A 5F Patentee before: Chia lung Biological Engineering (Wuhan) Co., Ltd. |