CN101935256A

CN101935256A - Pleurotus eryngii culture medium

Info

Publication number: CN101935256A
Application number: CN 201010294920
Authority: CN
Inventors: 于海龙; 李玉; 周峰; 郭倩; 陈有兴
Original assignee: Shanghai Guosen Biotechnology Co ltd; Shanghai Academy of Agricultural Sciences
Current assignee: Shanghai Guosen Biotechnology Co ltd; Shanghai Academy of Agricultural Sciences
Priority date: 2010-09-28
Filing date: 2010-09-28
Publication date: 2011-01-05

Abstract

The invention discloses a pleurotus eryngii culture medium, which contains walnut shells, bran, corn meal, rice bran and lime. The culture medium is used for culturing pleurotus eryngii, so the walnut shells can be recycled and the bioavailability of active ingredients in the walnut shells is improved; meanwhile, the production cost of the pleurotus eryngii is reduced and the economic benefit of pleurotus eryngii producers is increased.

Description

A kind of pleurotus eryngii cultivating material

Technical field

The present invention relates to field of edible fungus culture, relate in particular to a kind of pleurotus eryngii cultivating material that utilizes nut-shell for raw material.

Background technology

Pleurotus eryngii [ Plersotus eryngii(DC. Ex Fr.) Quel] have another name called eryngo and pick up the ears, be under the jurisdiction of Basidiomycotina, Hymenomycetes, Holobasidiomycetidae, Agaricales, Pleurotaceae, pleurotus.Pleurotus eryngii stem color and luster is snow-white, and quality is tender and crisp, and delicious flavour has almond flavor and abalone local flavor concurrently, have the good reputation of " flat mushroom king ", " King Boletus on the grassland ".In recent years studies show that, Pleurotus eryngii contains multiple amino acids and VITAMIN, nutritious, and have reducing blood-fat, hypotensive, decreasing cholesterol, promotion gastro-intestinal digestion, enhancing body immunizing power, prevent effects such as cardiovascular disorder, having DEVELOPMENT PROSPECT, is the rare edible mushrooms that Food and Argriculture OrganizationFAO (FAO) is recommended to various countries.

At present, China has become world's Edible Fungi first big country, and cultivated area is big, the ultimate production height.According to edible fungi of china association statistics, China's edible mushrooms output was 5.8 ten thousand tons in 1978, reached 2020.6 ten thousand tons in 2009.The price that meanwhile is used for the starting material of Edible Fungi such as wood chip, cotton seed hull, corn cob etc. skyrockets, price as present cotton seed hulls has reached 1500-2000 unit/ton, the cost of Edible Fungi rises steadily, seek that a kind of can completely or partially to substitute raw-material agricultural byproducts such as wood chip, cotton seed hull, corn cob significant, not only can reduce Edible Fungi person's production cost but also the agricultural byproduct recycle can be played the significance of energy-saving and emission-reduction and realization recycling economy.

Nut-shell be in the walnut kernel production process through degreasing, fragmentation, etc. the tankage that produce of processing process be difficult to direct utilization because its quality is hard, be mainly used in soaked, engraving and industrial filter membrane etc., its utilization ratio only 10%, major part is directly burnt.The nut-shell principal element is C, H, O, N, S etc., and contain trace elements such as calcium, magnesium, iron, silicon, phosphorus, main component is lignin, Mierocrystalline cellulose and hemicellulose, compare with other plant, hot water or cold water's extractum, 1%NaOH extractum, acid insoluble lignin content are all higher, content of cellulose is obviously lower, and many pentosan content and pourous wood are close.

Summary of the invention

Purpose of the present invention provides a kind of culture material of Pleurotus eryngii.

A kind of pleurotus eryngii cultivating material provided by the present invention contains following component and content (weight percent) and is: nut-shell 17-64%, wheat bran 10%, Semen Maydis powder 5%, rice bran 20%, lime 1%.

Can also contain wood chip, wherein the weight percent content of wood chip is 0.001-34%;

Can also contain corn cob, wherein the weight percent content of corn cob is 0.001-30%.

Optimized technical scheme is, a kind of pleurotus eryngii cultivating material, and it contains following component and content: nut-shell 17%, wood chip 17%, corn cob 30%, wheat bran 10%, Semen Maydis powder 5%, rice bran 20%, lime 1%.

With above-mentioned raw materials siccative thorough mixing, add water again and stir, making its final water content is 65-67%.It is standby promptly to can be used as culture material.

Wherein used nut-shell is that this pretreated method is with common nut-shell process pre-treatment:

The nut-shell of the getting walnut kernel back of being exposed to the sun is ground into the big particle of 5-10 mm in pulverizer, in clear water, soaked 24 hours then, will float on the part that the upper strata comprises walnut kernel and remove; Repeat to soak and remove upper strata liquid 2-3 time; At last with nut-shell drying, standby.

Described wood chip, corn cob, wheat bran, Semen Maydis powder, rice bran, gypsum are commercially available product.

The present invention has the following advantages:

1, nut-shell is the crops leftovers that produces in the walnut production process, and its abundant resource has not only made full use of effective constituent in the nut-shell, reduces environmental pollution, has improved economic benefit, also can save timber, the protection eubiosis.

2, utilize nut-shell partly to replace wood chip, corn cob and cotton seed hulls, can reduce above three kinds of raw-material consumptions, reduce production costs, simultaneously, nut-shell has bigger granularity, can increase the porosity of prescription, help accelerating to send out bacterium speed, shorten the production cycle, reduce the water power consumption, reduce production costs.

3, nut-shell was difficult to use owing to quality is hard in the past, the pretreatment process simple and fast of nut-shell mentioned herein, and cost is low, can large-scale promotion application.

4, use this culture material Xinbao mushroom culturing, can make the nut-shell recycle, improved the bioavailability of effective constituent in the nut-shell; Reduce the Pleurotus eryngii production cost simultaneously, increased the Pleurotus eryngii producer's economic interests.

Embodiment:

Embodiment 1:

Take by weighing nut-shell, wood chip, wheat bran, Semen Maydis powder, rice bran, lime, its weight percent is a nut-shell 30%, wood chip 34%, wheat bran 10%, Semen Maydis powder 5%, rice bran 20%, lime 1%.Elder generation's siccative stirs, the limit adds the waterside and is stirred to complete mixing again, make water content reach 66%, bottle through machine, about every bottled siccative 200g, 121 ℃ of autoclavings 2 hours, (culture condition is 23 ℃ of temperature to machine inoculation Pleurotus eryngii bacterial classification (the Pleurotus eryngii bacterial classification is apricot Korea Spro: provided by Edible Fungus Inst., Shanghai Academy of Agriculture) (inoculum size 3% V/V) through cultivating, humidity 65%-75%, CO ₂Concentration 1500ppm), (the fruiting condition is 16 ℃ of temperature to management of producing mushroom behind the mycelium stimulation, humidity 91%-95%, CO ₂Concentration 1500ppm-2500ppm) adds up its bacteria developing period, cultivation cycle, mushroom weight and biological transformation ratio.

Embodiment 2:

Take by weighing nut-shell, corn cob, wheat bran, Semen Maydis powder, rice bran, lime, its weight percent is a nut-shell 34%, corn cob 30%, wheat bran 10%, Semen Maydis powder 5%, rice bran 20%, lime 1%.Remaining is with embodiment 1.

Embodiment 3:

Take by weighing nut-shell, corn cob, wheat bran, Semen Maydis powder, rice bran, lime, its weight percent is a nut-shell 64%, corn cob 15%, wheat bran 10%, Semen Maydis powder 5%, rice bran 20%, lime 1%.Remaining is with embodiment 1.

Embodiment 4:

Take by weighing nut-shell, wood chip, corn cob, wheat bran, Semen Maydis powder, rice bran, lime, its weight percent is a nut-shell 17%, wood chip 17%, corn cob 30%, wheat bran 10%, Semen Maydis powder 5%, rice bran 20%, lime 1%.Remaining is with embodiment 1.

The pleurotus eryngii cultivating material prescription of embodiment 5:(routine is as the Comparative Examples of embodiment 1-4)

Take by weighing wood chip, corn cob, wheat bran, Semen Maydis powder, rice bran, lime, its weight percent is a wood chip 34%, corn cob 30%, wheat bran 10%, Semen Maydis powder 5%, rice bran 20%, lime 1%.Remaining is with embodiment 1.

The effect of above embodiment is as follows:

Handle	Bacteria developing period (d)	The cultivation cycle (d)	Mushroom heavy (g)	Biology efficient (%)
					Embodiment 1	33?	48?	130	65.00?
Embodiment 2	30	45?	120	60.00?
					Embodiment 3	29	44?	110?	55.00?
Embodiment 4	32	47?	135?	67.50
					Embodiment 5	35?	50?	125?	62.50

The result shows, compare with the effect that the pleurotus eryngii cultivating material of routine is cultivated, there is not significant difference, therefore, this prescription can be applied to the walnut producing region, with local affluent resources-nut-shell is starting material, and part replaces wood chip and corn cob to produce Pleurotus eryngii, plays the effect of recycling economy and peasant's increasing both production and income.

Claims

1. a pleurotus eryngii cultivating material is characterized in that containing following component and content: nut-shell 17-64%, wheat bran 10%, Semen Maydis powder 5%, rice bran 20%, lime 1%.

2. pleurotus eryngii cultivating material according to claim 1 is characterized in that also containing wood chip, and wherein the weight percent content of wood chip is 0.001-34%.

3. according to claim 1 or 2 each described pleurotus eryngii cultivating materials, it is characterized in that also containing corn cob, wherein the weight percent content of corn cob is 0.001-30%.

4. pleurotus eryngii cultivating material according to claim 1 is characterized in that containing following component and content: nut-shell 17%, wood chip 17%, corn cob 30%, wheat bran 10%, Semen Maydis powder 5%, rice bran 20%, lime 1%.