CN101933469B - Method for artificial mass propagation of rice field eel seedlings - Google Patents
Method for artificial mass propagation of rice field eel seedlings Download PDFInfo
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- CN101933469B CN101933469B CN2009100909348A CN200910090934A CN101933469B CN 101933469 B CN101933469 B CN 101933469B CN 2009100909348 A CN2009100909348 A CN 2009100909348A CN 200910090934 A CN200910090934 A CN 200910090934A CN 101933469 B CN101933469 B CN 101933469B
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- eel
- rice field
- oxytocic hormone
- hormone
- spawning
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention relates to a method for artificial mass propagation of rice field eel seedlings, and belongs to the technical field of scientific application of fishery. The method is characterized by applying oral oxytocic hormone to rice field eels, namely feeding parent rice field eels with a certain dosage of oxytocic hormone by using animal bait as a carrier of the oxytocic hormone; and the method comprises the following steps of: stopping feeding of both male and female parent rice field eels for 1 day before spawning induction; observing oestrus conditions of bubbling and nesting of the male and female rice field eels in 3 to 4 days after the male and female rice field eels ingest an oxytocic hormone liquid medicament, and surrounding and marking nests with eichhornia crassipes after the nesting of the parent rice field eels is finished, wherein an inner net cage in a paddy field is used as a spawning pond; and naturally inseminating the parent rice field eels in the spawning pond, breaking membranes by fertilized ova and emerging seedlings after 144 hours, inhabiting all fish fries in the eichhornia crassipes surrounding the nests of the parent rice field eels after the fish fries break the membranes after 96 hours, and collecting the rice field eel seedlings at the time. By applying the method of the invention, the spawning induction ratio of the parent rice field eels reaches 80 to 90 percent, the hatching rate of natural spawning and fertilization is over 95 percent and the collection ratio of the rice field eel seedlings is over 95 percent.
Description
Technical field:
The present invention relates to a kind of artificial method of breeding the swamp eel seed in batches, belong to the fishery science applied technical field.
Background technology:
Swamp eel is one of main famous-brand and high-quality fish of China, cultures development in recent years very rapidly, and it is mainly cultured the producing region and is dispersed throughout provinces such as Hubei, Hunan, Anhui, Jiangxi, Zhejiang.Culture of swamp eel has become the leading industry of some regional culture fisheries.
But in swamp eel scale artificial culture process, the seed of artificial breeding is shortage very, and the seedling that drops at present is main with wild swamp eel seed resource.Culture wild swamp eel seedling, exist on producing that to culture survival rate low, annual 4～June the introducing a fine variety the stage of raising and train of seedling, its lethality is up to up to more than 60; Wild seedling must just can be introduced a fine variety input after annual June simultaneously, causes the culture-cycle short, has only 4 months culturing time every year.Because wild seedling comes from different waters, be prone to cause the swamp eel disease cross-infection of zones of different in addition.Therefore, wild swamp eel seed all can not be satisfied the scale of culture of swamp eel and the requirement of industrialization at aspects such as the time of putting in a suitable place to breed, quality and quantity and sources.
Summary of the invention:
The purpose of this invention is to provide a kind of artificial method of breeding the swamp eel seed in batches, support the seedling requirement of eel to satisfy scale.
The present invention realizes above-mentioned purpose through following technical scheme.
(1) artificial induced spawning:
Adopt the method for the oral oxytocic hormone of swamp eel, promptly use the carrier of animal feed as oxytocic hormone, with oxytocic hormone by certain dosage close eel of throwing something and feeding;
A, hasten parturition before, female, male close eel was all stopped eating 1 day;
Synthesizing of B, oxytocic hormone soup: human chorionic gonadtropin and luteotropin releasing hormone d-ala analog are mixed promptly synthetic oxytocic hormone soup in physiological saline;
The average weight of close eel is a if wait to hasten parturition, and contains in then every 2ml oxytocic hormone soup:
Human chorionic gonadtropin: (a/1000) * 2000IU;
Luteotropin releasing hormone d-ala analog: (a/1000) * (6～8) μ g;
C, the oxytocic hormone soup is injected in the earthworm body cavity by the 2ml/ bar;
D, the earthworm that is full of the oxytocic hormone soup in the body cavity is thrown something and fed to the swamp eel parent by the feeding volume of 1 of every tail parent eel;
(2) preparation of spawning pond:
Spawning pond is a net cage in the rice field, with area 2～5m
2Net cage place in the rice field, the thick earth of 18～20cm is gone in tiling in net cage then, the water surface exceeds earth 8～10cm in the net cage;
(3) natural spawning and hatching:
Male and female parents eel is full of behind the earthworm of oxytocic hormone soup 3～4 days in the body cavity of ingesting, observe the situation of oestrusing that its Tu Pao nests, and after close eel nesting finishes, with water hyacinth the fish nest is surrounded and marked;
(4) collection of larval stages swamp eel seedling:
Naturally, after the insemination, its fertilized egg was that rupture of membranes is emerged after 144 hours to parent's eel, all perched after 96 hours at the prelarva rupture of membranes near the Eichhornia crassipes that is being surrounded on the close yellow eel nest, carried out the collection of young eel seedling at this moment in spawning pond.Concrete grammar is: preparing specification earlier is the seed collection plastic containers of 100cm * 80cm * 60em, injects the quick-fried air water of half volume in the container; Then near the Eichhornia crassipes the fish nest is mentioned rapidly and collect in the plastic containers water and swing back and forth, be attached to the swamp eel prelarva seedling of Eichhornia crassipes root with wash-out in seed.
The present invention has following beneficial effect compared with prior art:
Use method of the present invention, swamp eel parent's spawning rate reaches 80～90%; The incubation rate of natural spawning fertilization reaches more than 95%; Prelarva seed collection rate reaches more than 95%; Can be the swamp eel large-scale cultivation in enormous quantities, high-quality swamp eel prelarva seed is provided, have extraordinary application prospect.
Embodiment:
Embodiment of the present invention is an example with the Jianghan Plain area, can carry out according to the following steps:
(1) artificial induced spawning:
Hasten parturition for 2 times fraction of the year of one, be chosen in late May to early June for the first time, be chosen in late July to early August for the second time.Adopt the method for the oral oxytocic hormone of swamp eel, promptly use the carrier of animal feed as oxytocic hormone, with oxytocic hormone by certain dosage close eel of throwing something and feeding.
A, hasten parturition before, female, male close eel was all stopped eating 1 day; In 20 minutes, to be eaten up behind the assurance animal feed carrier input swamp eel parent fish rearing pond by close eel.
Synthesizing of B, oxytocic hormone soup: human chorionic gonadtropin and luteotropin releasing hormone d-ala analog are mixed promptly synthetic oxytocic hormone soup in physiological saline;
The average weight of close eel is a if wait to hasten parturition, and contains in then every 2ml oxytocic hormone soup:
Human chorionic gonadtropin: (a/1000) * 2000IU;
Luteotropin releasing hormone d-ala analog: (a/1000) * (6～8) μ g;
During preparation oxytocic hormone soup; Confirm to wait the average weight a that hastens parturition close eel earlier; Calculate by every kg parent eel body weight then, every preparation 2ml soup adds the human chorionic gonadtropin of (a/1000) * 2000IU and (a/1000) * (6～8) luteotropin releasing hormone d-ala analog of μ g.During operation, according to dosage human chorionic gonadtropin and luteotropin releasing hormone d-ala analog powder are dissolved in 0.6% Ren Shi (Ringer ' s) in the lump and get final product in the physiological saline.
C, the oxytocic hormone soup is injected in the earthworm body cavity that complete nothing is hindered and specification is consistent by the 2ml/ bar.
D, the earthworm that is full of the oxytocic hormone soup in the body cavity is thrown something and fed to the swamp eel parent by the feeding volume of 1 of every tail parent eel.
(2) preparation of spawning pond:
Adopt the wild egg-laying environment of manual simulation swamp eel, carry out natural insemination and hatching.Spawning pond is a net cage in the rice field, with area 2～5m
2Net cage place in the rice field, the thick earth of 18～20cm is gone in tiling in net cage then, ridge is than more than the high 20～30cm of earth horizontal plane in the net cage, earth surfaces is in shallow degree and floods in the net cage, the water surface exceeds earth 8～10cm in the net cage.
(3) natural spawning and hatching:
Male and female parents eel is full of behind the earthworm of oxytocic hormone soup 3～4 days in the body cavity of ingesting, observe the situation of oestrusing that its Tu Pao nests, and after close eel nesting finishes, with water hyacinth the fish nest is surrounded and marked.
(4) collection of larval stages swamp eel seedling:
After parent's eel was inseminated in spawning pond naturally, its fertilized egg was that rupture of membranes is emerged after 144 hours, and whether rupture of membranes is to confirm concrete swamp eel seedling acquisition time through young eel in the observation swamp eel fish nest.All perch after 96 hours near the Eichhornia crassipes that is being surrounded on the close yellow eel nest at the prelarva rupture of membranes, carry out the collection of young eel seedling this moment.Concrete grammar is: preparing specification earlier is the seed collection plastic containers of 100cm * 80cm * 60cm, injects the quick-fried air water of half volume in the container; Then near the Eichhornia crassipes the fish nest is mentioned rapidly and collect in the plastic containers water and swing back and forth, be attached to the swamp eel prelarva seedling of Eichhornia crassipes root with wash-out in seed.
Claims (2)
1. artificial method of breeding the swamp eel seed in batches is characterized in that: may further comprise the steps:
(1) artificial induced spawning:
Adopt the method for the oral oxytocic hormone of swamp eel, promptly use the carrier of animal feed as oxytocic hormone, with oxytocic hormone by certain dosage close eel of throwing something and feeding;
A, hasten parturition before, female, male close eel was all stopped eating 1 day;
Synthesizing of B, oxytocic hormone soup: human chorionic gonadtropin and luteotropin releasing hormone d-ala analog are mixed promptly synthetic oxytocic hormone soup in physiological saline;
The average weight of close eel is a if wait to hasten parturition, and contains in then every 2ml oxytocic hormone soup:
Human chorionic gonadtropin: (a/1000) * 2000IU;
Luteotropin releasing hormone d-ala analog: (a/1000) * (6～8) μ g;
C, the oxytocic hormone soup is injected in the earthworm body cavity by the 2ml/ bar;
D, the earthworm that is full of the oxytocic hormone soup in the body cavity is thrown something and fed to the swamp eel parent by the feeding volume of 1 of every tail parent eel;
(2) preparation of spawning pond:
Spawning pond is a net cage in the rice field, with area 2～5m
2Net cage place in the rice field, the thick earth of 18～20cm is gone in tiling in net cage then, the water surface exceeds earth 8～10cm in the net cage;
(3) natural spawning and hatching:
Male and female parents eel is full of behind the earthworm of oxytocic hormone soup 3～4 days in the body cavity of ingesting, observe the situation of oestrusing that its Tu Pao nests, and after close eel nesting finishes, with water hyacinth the fish nest is surrounded and marked;
(4) collection of larval stages swamp eel seedling:
Naturally, after the insemination, its fertilized egg was that rupture of membranes is emerged after 144 hours to parent's eel, all perched after 96 hours at the prelarva rupture of membranes near the Eichhornia crassipes that is being surrounded on the close yellow eel nest, carried out the collection of young eel seedling at this moment in spawning pond.
2. a kind of artificial method of breeding the swamp eel seed in batches according to claim 1; The collection method that it is characterized in that its young eel seedling is: preparing specification earlier is the seed collection plastic containers of 100cm * 80cm * 60cm, injects the quick-fried air water of half volume in the container; Then near the Eichhornia crassipes the fish nest is mentioned rapidly and collect in the plastic containers water and swing back and forth, be attached to the swamp eel prelarva seedling of Eichhornia crassipes root with wash-out in seed.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2009100909348A CN101933469B (en) | 2009-08-14 | 2009-08-14 | Method for artificial mass propagation of rice field eel seedlings |
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| CN2009100909348A CN101933469B (en) | 2009-08-14 | 2009-08-14 | Method for artificial mass propagation of rice field eel seedlings |
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| CN101933469A CN101933469A (en) | 2011-01-05 |
| CN101933469B true CN101933469B (en) | 2012-08-15 |
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| CN2009100909348A Expired - Fee Related CN101933469B (en) | 2009-08-14 | 2009-08-14 | Method for artificial mass propagation of rice field eel seedlings |
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103004649A (en) * | 2011-09-23 | 2013-04-03 | 长江大学 | Ecology regulation and control method for delaying sex reversal time of eels |
| CN104273058B (en) * | 2013-07-12 | 2016-08-10 | 长江大学 | A kind of method of Monopterus albus (Zuiew) male parent's directive breeding |
| CN104335928B (en) * | 2013-07-25 | 2016-05-11 | 长江大学 | A kind of method of artificial bionic state breeding swamp eel seed |
| CN105613352B (en) * | 2014-10-26 | 2019-06-07 | 蒋石容 | A kind of apparatus and method that the artificial incubation of swamp eel fertilized eggs is educated with eel kind without earth culture |
| CN104542373A (en) * | 2014-11-30 | 2015-04-29 | 刘长华 | Finless eel fry culture method |
| CN104798704B (en) * | 2015-04-09 | 2017-09-22 | 华中农业大学 | A kind of large-scale artificial hatching method of swamp eel embryonated egg |
| CN106069951A (en) * | 2016-07-22 | 2016-11-09 | 仙桃市西流河国兵水产养殖专业合作社 | A kind of method that Monopterus albus (Zuiew) Ecology has soil to breed |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101103711A (en) * | 2007-07-30 | 2008-01-16 | 华中农业大学 | Artificial regulatory method for increasing female rice field eel fecundity |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101103711A (en) * | 2007-07-30 | 2008-01-16 | 华中农业大学 | Artificial regulatory method for increasing female rice field eel fecundity |
Non-Patent Citations (3)
| Title |
|---|
| 杨劲松.黄鳝的人繁及苗种培育试验.《科学养鱼》.2005,全文. * |
| 胡宝林.黄鳝的人工繁殖及苗种培育技术.《渔业致富指南》.2003,全文. * |
| 邴旭文.模仿自然繁殖条件下的黄鳝人工繁育试验.《水产学报》.2005,全文. * |
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