CN104542373A - Finless eel fry culture method - Google Patents

Abstract

The invention relates to the field of aquatic product reproduction, in particular to a finless eel fry culture method. According to the method, an artificial fertilization method is adopted, parent eels are selected, then, the parent eels are firstly subjected to maturation acceleration, gonadotrophin releasing hormone No.4 is injected into the parent eels, the pituitaries of the parent eels are prompted to secrete metakentrin and follicle stimulating hormone, ovulation and spermiation are induced, and the quality and the level of the parent gonad development can be obviously improved, so that the fertilization rate and the success rate of breeding can be improved during the artificial fertilization.

Description

A kind of swamp eel seedling-cultivating method

Technical field

The present invention relates to aquatic products and breed field, relate to a kind of swamp eel seedling-cultivating method in particular.

Technical background

Swamp eel has another name called yellow eel, Changyu etc., and be the excellent freshwater fish kind of a kind of high protein, low fat, high nutritive value, market comsupton all the time has higher demand to swamp eel.The swamp eel one in current supply market is from propagating artificially, two is from wild seizure, but the wild resource of swamp eel is day by day deficient, the swamp eel quantity of natural seizure reduces day by day, specification is also more and more less, in intensive scale artificial culture, just culture of swamp eel upsurge has been there is in China from early eighties in last century, but to the nineties, due to swamp eel seed scarcity of resources, the restriction of the problems such as cultural technique falls behind, many producers stop figthing mostly, individual cultivation on a small scale mostly, no matter in cultural technique, the aspects such as economic benefit are also in the reduced levels stage, also do not reach a high level, the cultivation level of scale, therefore the swamp eel market price is also relatively high.The factor of restriction swamp eel large-scale cultivation, except these factors of cultural technique, feed or disease, topmost is exactly seed problem, becomes the bottleneck of swamp eel large-scale cultivation.This is with the distinctive reproductive habit of swamp eel, physiological property is closely related, the fish brood amount of swamp eel is very low, generally only have hundreds of ovum, and in the gonad development process of swamp eel, there is a kind of special sex reversal phenomenon, what these breeding physiology characteristics had a strong impact on and constrained swamp eel breeds power, current swamp eel artificial feeding or the seed supported temporarily mainly catch by field, and batch production whole artificial propagation technology that will be ripe as the chinese carp artificial propagation not yet maturation reach a standard, the extensive seed supported needed for eel is difficult to stablize to be provided in a large number, eel seedling is not enough, what strongly limit that swamp eel raises on a large scale carries out.

Summary of the invention

The technical problem to be solved in the present invention is to provide a kind of swamp eel seedling-cultivating method.

For solving the problems of the technologies described above, the invention provides a kind of swamp eel seedling-cultivating method, comprising the following steps:

(1) selection of close eel and raising: select physique healthy and strong, travelling active, male eel body long 45 centimetres, body weight 100-120 gram; Female eel body long 40 centimetres, body weight 80-100 gram, then put in disinfectant after soaking 10-15 and put into rearing pool, animal bait of throwing something and feeding every day, each feeding volume accounts for the 3-6% raising close eel weight;

(2) to hasten parturition breeding: to close eel injection No. 4, gonadotropin-releasing hormone (GRH), dosage shot 5-8 microgram/kg body weight, can inject at twice, first pin 0.5-1.0 microgram, second pin 5-8 microgram/kg body weight, needle gage is 6-36 hour, during injection, the degree of depth is 0.5 centimetre, puts into cement pit and support temporarily after injection, and changes water every day once;

(3) artificial insemination: the female eel starting to ovulate is taken out, extrudes belly with hand, continuously extruded 2-3 time, until by the ovum crowded empty of the inside, then male eel is killed, take out spermary, as sperm is normal, with scissors, spermary is shredded, put into the ovum extruded, fully stir, then add Ren Shi solution 150-170 milliliter and place 10-15 minute, add clear water choosing again and remove spermary fragment and blood stains, put Lentic hatching in incubator.

(4) nursery is cultivated: nursery is put into nursery and cultivate pond, throw something and feed open-mouthed bait and zooplankton, and every 2 days change water once, and after 1-2 month, eel seedling grows to 10 cm and namely can be moved to into the raising of eel pond.

Further, the stirring ratio of described male and female ovum is 3:1.

The beneficial effect of employing technique scheme is: a kind of swamp eel seedling-cultivating method of the present invention, adopt the method for artificial insemination, in the selection to close eel with first accelerate the ripening to close eel, to close eel injection No. 4, gonadotropin-releasing hormone (GRH), impel swamp eel pituitary luteotropin and short filter follicle maturity element, induced ovulation and ejaculation, can significantly improve quality and the level of parent's gonad development, thus can make to improve the fertilization rate of breeding and success rate when artificial insemination.

Embodiment

Embodiment 1

A kind of swamp eel seedling-cultivating method, comprises the following steps:

(1) selection of close eel and raising: select physique healthy and strong, travelling active, male eel body long 45 centimetres, body weight 100 grams; Female eel body long 40 centimetres, body weight 80 grams, then put into after soaking 10 in disinfectant and put into rearing pool, animal bait of throwing something and feeding every day, and each feeding volume accounts for raises 3% of close eel weight;

(2) to hasten parturition breeding: to close eel injection No. 4, gonadotropin-releasing hormone (GRH), a dosage shot 5 micrograms/kg body weight, can inject at twice, first pin 0.5 microgram, second pin 5 micrograms/kg body weight, needle gage is 6 hours, during injection, the degree of depth is 0.5 centimetre, puts into cement pit and support temporarily after injection, and changes water every day once;

(3) artificial insemination: the female eel starting to ovulate is taken out, extrudes belly with hand, continuously extruded 2 times, until by the ovum crowded empty of the inside, then male eel is killed, take out spermary, as sperm is normal, with scissors, spermary is shredded, put into the ovum extruded, fully stir, then add Ren Shi solution 150 milliliters and place 10 minutes, add clear water choosing again and remove spermary fragment and blood stains, put Lentic hatching in incubator.

(4) nursery is cultivated: nursery is put into nursery and cultivate pond, throw something and feed open-mouthed bait and zooplankton, and every 2 days change water once, and after 1 month, eel seedling grows to 10 cm and namely can be moved to into the raising of eel pond.

Further, the stirring ratio of described male and female ovum is 3:1.

Embodiment 2

A kind of swamp eel seedling-cultivating method, comprises the following steps:

(1) selection of close eel and raising: select physique healthy and strong, travelling active, male eel body long 45 centimetres, body weight 120 grams; Female eel body long 40 centimetres, body weight 100 grams, then put into after soaking 15 in disinfectant and put into rearing pool, animal bait of throwing something and feeding every day, and each feeding volume accounts for raises 6% of close eel weight;

(2) to hasten parturition breeding: to close eel injection No. 4, gonadotropin-releasing hormone (GRH), a dosage shot 8 micrograms/kg body weight, can inject at twice, first pin 1.0 microgram, second pin 8 micrograms/kg body weight, needle gage is 36 hours, during injection, the degree of depth is 0.5 centimetre, puts into cement pit and support temporarily after injection, and changes water every day once;

(3) artificial insemination: the female eel starting to ovulate is taken out, extrudes belly with hand, continuously extruded 3 times, until by the ovum crowded empty of the inside, then male eel is killed, take out spermary, as sperm is normal, with scissors, spermary is shredded, put into the ovum extruded, fully stir, then add Ren Shi solution 170 milliliters and place 15 minutes, add clear water choosing again and remove spermary fragment and blood stains, put Lentic hatching in incubator.

(4) nursery is cultivated: nursery is put into nursery and cultivate pond, throw something and feed open-mouthed bait and zooplankton, and every 2 days change water once, and after 2 months, eel seedling grows to 10 cm and namely can be moved to into the raising of eel pond.

Further, the stirring ratio of described male and female ovum is 3:1.

Claims (2)

1. a swamp eel seedling-cultivating method, is characterized in that: comprise the following steps:

(1) selection of close eel and raising: select physique healthy and strong, travelling active, male eel body long 45 centimetres, body weight 100-120 gram; Female eel body long 40 centimetres, body weight 80-100 gram, then put in disinfectant after soaking 10-15 and put into rearing pool, animal bait of throwing something and feeding every day, each feeding volume accounts for the 3-6% raising close eel weight;

(2) to hasten parturition breeding: to close eel injection No. 4, gonadotropin-releasing hormone (GRH), dosage shot 5-8 microgram/kg body weight, can inject at twice, first pin 0.5-1.0 microgram, second pin 5-8 microgram/kg body weight, needle gage is 6-36 hour, during injection, the degree of depth is 0.5 centimetre, puts into cement pit and support temporarily after injection, and changes water every day once;

(3) artificial insemination: the female eel starting to ovulate is taken out, extrudes belly with hand, continuously extruded 2-3 time, until by the ovum crowded empty of the inside, then male eel is killed, take out spermary, as sperm is normal, with scissors, spermary is shredded, put into the ovum extruded, fully stir, then add Ren Shi solution 150-170 milliliter and place 10-15 minute, add clear water choosing again and remove spermary fragment and blood stains, put Lentic hatching in incubator;

(4) nursery is cultivated: nursery is put into nursery and cultivate pond, throw something and feed open-mouthed bait and zooplankton, and every 2 days change water once, and after 1-2 month, eel seedling grows to 10 cm and namely can be moved to into the raising of eel pond.

2. a kind of swamp eel seedling-cultivating method according to claim 1, is characterized in that: the stirring ratio of described male and female ovum is 3:1.