CN101868475A - 骨吸收抑制用食品原材料 - Google Patents
骨吸收抑制用食品原材料 Download PDFInfo
- Publication number
- CN101868475A CN101868475A CN200880114098A CN200880114098A CN101868475A CN 101868475 A CN101868475 A CN 101868475A CN 200880114098 A CN200880114098 A CN 200880114098A CN 200880114098 A CN200880114098 A CN 200880114098A CN 101868475 A CN101868475 A CN 101868475A
- Authority
- CN
- China
- Prior art keywords
- protein fraction
- milk protein
- bone resorption
- resolvent
- fraction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 208000006386 Bone Resorption Diseases 0.000 title claims abstract description 59
- 230000024279 bone resorption Effects 0.000 title claims abstract description 59
- 230000002401 inhibitory effect Effects 0.000 title description 21
- 235000013305 food Nutrition 0.000 title description 8
- 239000000463 material Substances 0.000 title description 8
- 102000014171 Milk Proteins Human genes 0.000 claims abstract description 97
- 108010011756 Milk Proteins Proteins 0.000 claims abstract description 97
- 235000021239 milk protein Nutrition 0.000 claims abstract description 97
- 235000001014 amino acid Nutrition 0.000 claims abstract description 26
- 150000001413 amino acids Chemical class 0.000 claims abstract description 26
- 239000000203 mixture Substances 0.000 claims abstract description 22
- 235000013336 milk Nutrition 0.000 claims abstract description 15
- 210000004080 milk Anatomy 0.000 claims abstract description 15
- 239000008267 milk Substances 0.000 claims abstract description 15
- 230000000452 restraining effect Effects 0.000 claims abstract description 10
- 230000002378 acidificating effect Effects 0.000 claims abstract description 6
- 229940078581 Bone resorption inhibitor Drugs 0.000 claims description 12
- 239000003814 drug Substances 0.000 claims description 12
- 235000005911 diet Nutrition 0.000 claims description 10
- 230000037213 diet Effects 0.000 claims description 10
- 108091005804 Peptidases Proteins 0.000 claims description 4
- 102000035195 Peptidases Human genes 0.000 claims description 4
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 claims description 4
- 230000002265 prevention Effects 0.000 abstract description 4
- 210000000988 bone and bone Anatomy 0.000 description 18
- ZAHDXEIQWWLQQL-IHRRRGAJSA-N Deoxypyridinoline Chemical compound OC(=O)[C@@H](N)CCCC[N+]1=CC(O)=C(C[C@H](N)C([O-])=O)C(CC[C@H](N)C(O)=O)=C1 ZAHDXEIQWWLQQL-IHRRRGAJSA-N 0.000 description 15
- 230000000694 effects Effects 0.000 description 15
- 238000000034 method Methods 0.000 description 14
- DDRJAANPRJIHGJ-UHFFFAOYSA-N creatinine Chemical compound CN1CC(=O)NC1=N DDRJAANPRJIHGJ-UHFFFAOYSA-N 0.000 description 12
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 10
- 235000018102 proteins Nutrition 0.000 description 10
- 102000004169 proteins and genes Human genes 0.000 description 10
- 108090000623 proteins and genes Proteins 0.000 description 10
- 210000002700 urine Anatomy 0.000 description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 9
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 8
- 208000001132 Osteoporosis Diseases 0.000 description 8
- 210000000481 breast Anatomy 0.000 description 8
- 239000011575 calcium Substances 0.000 description 8
- 229910052791 calcium Inorganic materials 0.000 description 8
- 238000012360 testing method Methods 0.000 description 8
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 229940109239 creatinine Drugs 0.000 description 6
- 239000000843 powder Substances 0.000 description 6
- 150000003839 salts Chemical class 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 238000005728 strengthening Methods 0.000 description 6
- 108010046377 Whey Proteins Proteins 0.000 description 5
- 102000007544 Whey Proteins Human genes 0.000 description 5
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 description 5
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 5
- 239000000872 buffer Substances 0.000 description 5
- 239000000470 constituent Substances 0.000 description 5
- 238000010612 desalination reaction Methods 0.000 description 5
- 238000001223 reverse osmosis Methods 0.000 description 5
- 238000004659 sterilization and disinfection Methods 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 4
- 241000700159 Rattus Species 0.000 description 4
- 239000005862 Whey Substances 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 239000008367 deionised water Substances 0.000 description 4
- 229910021641 deionized water Inorganic materials 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 239000011347 resin Substances 0.000 description 4
- 229920005989 resin Polymers 0.000 description 4
- 229920001661 Chitosan Polymers 0.000 description 3
- 101000955144 Drosophila melanogaster WASH complex subunit 1 Proteins 0.000 description 3
- 241000208202 Linaceae Species 0.000 description 3
- 235000004431 Linum usitatissimum Nutrition 0.000 description 3
- 108010019160 Pancreatin Proteins 0.000 description 3
- 235000010724 Wisteria floribunda Nutrition 0.000 description 3
- 239000012491 analyte Substances 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 238000000909 electrodialysis Methods 0.000 description 3
- 239000000835 fiber Substances 0.000 description 3
- 229910052500 inorganic mineral Inorganic materials 0.000 description 3
- 235000012204 lemonade/lime carbonate Nutrition 0.000 description 3
- 239000011707 mineral Substances 0.000 description 3
- 235000010755 mineral Nutrition 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 229940055695 pancreatin Drugs 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 239000007858 starting material Substances 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 238000006277 sulfonation reaction Methods 0.000 description 3
- 238000009941 weaving Methods 0.000 description 3
- WIGIZIANZCJQQY-UHFFFAOYSA-N 4-ethyl-3-methyl-N-[2-[4-[[[(4-methylcyclohexyl)amino]-oxomethyl]sulfamoyl]phenyl]ethyl]-5-oxo-2H-pyrrole-1-carboxamide Chemical compound O=C1C(CC)=C(C)CN1C(=O)NCCC1=CC=C(S(=O)(=O)NC(=O)NC2CCC(C)CC2)C=C1 WIGIZIANZCJQQY-UHFFFAOYSA-N 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 235000010469 Glycine max Nutrition 0.000 description 2
- 244000068988 Glycine max Species 0.000 description 2
- 101710093543 Probable non-specific lipid-transfer protein Proteins 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 244000309466 calf Species 0.000 description 2
- 238000005341 cation exchange Methods 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 235000013351 cheese Nutrition 0.000 description 2
- 239000013078 crystal Substances 0.000 description 2
- 230000009849 deactivation Effects 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 229940088598 enzyme Drugs 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- -1 lime carbonate Chemical class 0.000 description 2
- 230000011164 ossification Effects 0.000 description 2
- 210000000963 osteoblast Anatomy 0.000 description 2
- 210000002997 osteoclast Anatomy 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 230000035755 proliferation Effects 0.000 description 2
- 210000000582 semen Anatomy 0.000 description 2
- 235000013599 spices Nutrition 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 210000002784 stomach Anatomy 0.000 description 2
- 235000000346 sugar Nutrition 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
- 235000002687 Caesalpinia echinata Nutrition 0.000 description 1
- 102000055006 Calcitonin Human genes 0.000 description 1
- 108060001064 Calcitonin Proteins 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 241000127464 Paubrasilia echinata Species 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- OFHCOWSQAMBJIW-AVJTYSNKSA-N alfacalcidol Chemical compound C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C\C=C1\C[C@@H](O)C[C@H](O)C1=C OFHCOWSQAMBJIW-AVJTYSNKSA-N 0.000 description 1
- 235000012538 ammonium bicarbonate Nutrition 0.000 description 1
- 150000003863 ammonium salts Chemical class 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 235000015895 biscuits Nutrition 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- BBBFJLBPOGFECG-VJVYQDLKSA-N calcitonin Chemical compound N([C@H](C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC=1NC=NC=1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H]([C@@H](C)O)C(=O)N1[C@@H](CCC1)C(N)=O)C(C)C)C(=O)[C@@H]1CSSC[C@H](N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)O)C(=O)N1 BBBFJLBPOGFECG-VJVYQDLKSA-N 0.000 description 1
- 229960004015 calcitonin Drugs 0.000 description 1
- 239000001506 calcium phosphate Substances 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 235000011010 calcium phosphates Nutrition 0.000 description 1
- 159000000007 calcium salts Chemical class 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- OEYIOHPDSNJKLS-UHFFFAOYSA-N choline Chemical compound C[N+](C)(C)CCO OEYIOHPDSNJKLS-UHFFFAOYSA-N 0.000 description 1
- 229960001231 choline Drugs 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 235000020247 cow milk Nutrition 0.000 description 1
- 235000013365 dairy product Nutrition 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 210000003278 egg shell Anatomy 0.000 description 1
- 238000004945 emulsification Methods 0.000 description 1
- 230000001804 emulsifying effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000029142 excretion Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000009246 food effect Effects 0.000 description 1
- 235000015203 fruit juice Nutrition 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 235000021552 granulated sugar Nutrition 0.000 description 1
- 235000020256 human milk Nutrition 0.000 description 1
- 210000004251 human milk Anatomy 0.000 description 1
- 238000010191 image analysis Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 238000009806 oophorectomy Methods 0.000 description 1
- 210000001672 ovary Anatomy 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 235000015927 pasta Nutrition 0.000 description 1
- 239000002831 pharmacologic agent Substances 0.000 description 1
- 150000003016 phosphoric acids Chemical class 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000002787 reinforcement Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000012266 salt solution Substances 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 238000007493 shaping process Methods 0.000 description 1
- 235000020183 skimmed milk Nutrition 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- 210000004872 soft tissue Anatomy 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 239000003826 tablet Substances 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 1
- 229940038773 trisodium citrate Drugs 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- 235000019166 vitamin D Nutrition 0.000 description 1
- 239000011710 vitamin D Substances 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
- 235000015099 wheat brans Nutrition 0.000 description 1
- 235000021119 whey protein Nutrition 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/40—Feeding-stuffs specially adapted for particular animals for carnivorous animals, e.g. cats or dogs
-
- A—HUMAN NECESSITIES
- A21—BAKING; EDIBLE DOUGHS
- A21D—TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
- A21D2/00—Treatment of flour or dough by adding materials thereto before or during baking
- A21D2/08—Treatment of flour or dough by adding materials thereto before or during baking by adding organic substances
- A21D2/24—Organic nitrogen compounds
- A21D2/26—Proteins
- A21D2/261—Animal proteins
- A21D2/263—Animal proteins from dairy products
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C19/00—Cheese; Cheese preparations; Making thereof
- A23C19/06—Treating cheese curd after whey separation; Products obtained thereby
- A23C19/068—Particular types of cheese
- A23C19/08—Process cheese preparations; Making thereof, e.g. melting, emulsifying, sterilizing
- A23C19/082—Adding substances to the curd before or during melting; Melting salts
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/20—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from milk, e.g. casein; from whey
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/341—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins
- A23J3/343—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins of dairy proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/385—Concentrates of non-alcoholic beverages
- A23L2/39—Dry compositions
- A23L2/395—Dry compositions in a particular shape or form
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
- A23L2/66—Proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/19—Dairy proteins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/20—Milk; Whey; Colostrum
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P19/00—Drugs for skeletal disorders
- A61P19/08—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4702—Regulators; Modulating activity
- C07K14/4703—Inhibitors; Suppressors
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Nutrition Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Animal Husbandry (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Virology (AREA)
- Birds (AREA)
- Mycology (AREA)
- Molecular Biology (AREA)
- Physical Education & Sports Medicine (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Developmental Biology & Embryology (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Epidemiology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Orthopedic Medicine & Surgery (AREA)
- Rheumatology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
本发明的具有以下(1)~(4)特性的乳蛋白质级分,其骨吸收抑制作用优异、在骨疾病的预防或治疗中非常有用。(1)其为乳来源。(2)其为含有分子量在12,000~16,000道尔顿范围内的蛋白质的级分。(3)构成氨基酸的组成中含有18~20重量%的碱性氨基酸、且碱性氨基酸/酸性氨基酸的比在0.7~0.9的范围内。(4)具有骨吸收抑制作用。
Description
技术领域
本发明涉及具有骨吸收抑制作用的乳蛋白质级分或乳蛋白质级分分解物。
本发明的乳蛋白质级分或乳蛋白质级分分解物具有骨吸收抑制作用,因而作为以骨疾病的预防或治疗、骨强化等为目的的骨吸收抑制剂以及作为用于骨疾病的预防或治疗或者抑制骨吸收的药品、饮食品和饲料的有效成分非常有用。
背景技术
近年来,伴随着高龄化,骨质疏松症、骨折、腰痛等各种骨疾病具有增加的倾向。在骨组织中,不断进行着骨生成和骨吸收,在年轻时骨生成和骨吸收保持着良好的平衡,但随着年龄的增长,由于各种原因该平衡倾向于骨吸收(解偶联)。并且,长时间持续该状态的话会使得骨组织变脆,导致发生骨质疏松症、骨折、腰痛等各种骨疾病。认为如果能够防止该解偶联,则能够预防骨质疏松症、骨折、腰痛等各种骨疾病。
以往,作为防止解偶联、预防或治疗各种骨疾病的方法,曾进行了以下方法:(1)食物补钙,(2)轻微运动,(3)日光浴,(4)药物治疗等。对于食物补钙,使用碳酸钙、磷酸钙等钙盐以及蛋壳、鱼骨粉等天然钙制剂。然而,它们未必能称作适合经口摄取的原材料。对于轻微运动而言,认为慢跑或散步等较佳,但体弱的情况下即便是轻微的运动也非常麻烦,而且卧床的老人更是几乎无法运动。从补充活化维生素D3的观点来看,日光浴是很好的,但仅靠日光浴是不够的。药物投与中使用1α-羟基维生素D3、降钙素制剂等,已知其对骨质疏松症的治疗是有效的。然而,这些物质本身是药品,不能作为食品原材料使用。
本发明人等为了获得能够作为食品原材料使用的具有骨强化作用的物质,对存在于乳中的骨强化因子进行了持续的探索。结果发现,从乳清蛋白质的水溶性级分除去了乳清来源的盐而得的蛋白质和肽混合物具有骨强化作用(例如,参照专利文献1)。并且,发现对该蛋白质和肽混合物的水溶液进行乙醇处理、加热处理、加盐处理、超滤膜处理而得的级分具有促进成骨细胞增殖的作用和骨强化作用(例如,参照专利文献2、3)。另外,发现存在于乳中的碱性蛋白质具有促进成骨细胞增殖的作用、骨强化作用和防止骨吸收的作用(例如,参照专利文献4)。
专利文献1:日本专利3160862号公报
专利文献2:日本专利3092874号公报
专利文献3:日本特开平5-320066号公报
专利文献4:日本专利3112637号公报
发明内容
发明要解决的问题
本发明发现了一种能够作为食品原材料使用的、具有骨吸收抑制作用的乳蛋白质级分或乳蛋白质级分分解物,其课题在于提供一种含有该具有骨吸收抑制作用的乳蛋白质级分或乳蛋白质级分分解物的骨吸收抑制剂,以及提供配合有具有骨吸收抑制作用的乳蛋白质级分或乳蛋白质级分分解物的药品、饮食品和饲料。
用于解决问题的方案
本发明人等尝试对新的骨吸收抑制原材料进行探索,结果发现获得了与以往的食品原材料相比显示出更强的骨吸收抑制作用的级分。并且,基于这些见解,以致获得了含有该具有骨吸收抑制作用的乳蛋白质级分或乳蛋白质级分分解物的骨吸收抑制剂、以及配合有具有骨吸收抑制作用的乳蛋白质级分或乳蛋白质级分分解物的药品、饮食品和饲料。
本发明为包含下述方案的发明。
(A)涉及一种具有以下特性的乳蛋白质级分:
(1)其为乳来源;
(2)其为含有在十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)中分子量在12,000~16,000道尔顿范围内的蛋白质的级分;
(3)构成氨基酸的组成中含有18~20重量%的碱性氨基酸、且碱性氨基酸/酸性氨基酸的比在0.7~0.9的范围内;
(4)具有骨吸收抑制作用。
(B)一种乳蛋白质级分分解物,其通过用蛋白酶将前述乳蛋白质级分分解而获得。
(C)一种骨吸收抑制剂,其含有(A)所述的乳蛋白质级分或(B)所述的乳蛋白质级分分解物。
(D)一种骨吸收抑制用药品,其配合有(A)所述的乳蛋白质级分或(B)所述的乳蛋白质级分分解物。
(E)一种骨吸收抑制用饮食品,其配合有(A)所述的乳蛋白质级分或(B)所述的乳蛋白质级分分解物。
(F)一种骨吸收抑制用饲料,其配合有(A)所述的乳蛋白质级分或(B)所述的乳蛋白质级分分解物。
发明的效果
本发明的以具有骨吸收抑制作用的乳蛋白质级分或乳蛋白质级分分解物为有效成分的骨吸收抑制剂、以及配合有具有骨吸收抑制作用的乳蛋白质级分或乳蛋白质级分分解物的药品、饮食品和饲料,通过经口摄取,可在体内抑制骨吸收。因此,本发明的以乳蛋白质级分为有效成分的骨吸收抑制剂、以及配合有具有骨吸收抑制作用的乳蛋白质级分的药品、饮食品和饲料,由于可在人或动物的生物体内抑制骨吸收,因而具有骨强化作用,且对于抑制骨质疏松症等骨量减少有效。
附图说明
图1所示为测定尿中骨吸收标志即脱氧吡啶啉(deoxypyridinoline,DPD)量的尿中总量和肌酸酐所对应的DPD浓度的结果的图(试验例2)。
具体实施方式
本发明涉及以具有骨吸收抑制作用的乳蛋白质级分或乳蛋白质级分分解物为有效成分的骨吸收抑制剂、以及配合有具有骨吸收抑制作用的乳蛋白质级分或乳蛋白质级分分解物的药品、饮食品和饲料。
本发明的这种乳蛋白质级分例如能够通过如下方式获得:使脱脂乳或乳清等乳原料与阳离子交换树脂接触,用0.3M的食盐溶液清洗后,用0.6M的食盐洗脱液使吸附于该树脂上的乳蛋白质洗脱从而获得。另外,作为盐类,除了食盐以外可以使用钾盐、铵盐、磷酸盐、醋酸盐、碳酸盐等,通过将清洗液的离子强度适当调整为0.3~0.4、将洗脱溶液的离子强度适当调整为0.55~0.65,能够获得本发明的乳蛋白质级分。另外,还可以通过将该洗脱级分回收,利用反渗透(RO)膜或电渗析(ED)法等进行脱盐和浓缩,并根据需要进行干燥来获得。作为反渗透(RO)膜,可以例示Desal-3(Desalination公司制)、HR-95(Dow Danmark公司制)、NTR-729HF(日东电工公司制)等。另外,电渗析(ED)装置可以例示Yuasa Ionics Inc.或日本炼水公司制的电渗析装置。
另外,作为获得乳来源的微量蛋白质级分的方法,已知有如下方法:在使乳或乳来源的原料与阳离子交换体接触后,用pH超过5、且离子强度超过0.5的洗脱液使吸附于该阳离子交换体上的碱性蛋白质级分洗脱,从而获得乳来源的微量蛋白质级分的方法〔日本特开平5-202098号公报〕;使用海藻酸凝胶而获得的方法〔日本特开昭61-246198号公报〕;使用无机多孔性颗粒从乳清中获得的方法〔日本特开平1-86839号公报〕;使用硫酸化酯化合物从乳中获得的方法〔日本特开昭63-255300号公报〕等。本发明中,以这样的方法可以获得乳来源的蛋白质级分。
这样回收的乳蛋白质级分通常可以通过冷冻干燥等粉末化后使用。
本发明中使用的具有骨吸收抑制作用的乳蛋白质级分优选其构成氨基酸的组成中含有18~20重量%的碱性氨基酸、且碱性氨基酸/酸性氨基酸的比在0.7~0.9的范围内。若在该范围之外的话,无法发挥本申请发明的效果。另外,本发明品为以分子量12,000~16,000道尔顿、等电点10以上的蛋白质为主要成分的蛋白质混合物。
乳蛋白质级分分解物具有与乳蛋白质级分同样的氨基酸组成,例如,可以通过使胃蛋白酶、胰蛋白酶、胰凝乳蛋白酶等蛋白酶作用于通过上述方法获得的乳蛋白质级分,进而根据需要用胰酶等蛋白酶作用,从而能够获得平均分子量在4,000以下的分解物。另外,乳蛋白质级分分解物通常可以通过冷冻干燥等粉末化后使用。
作为成为具有骨吸收抑制作用的乳蛋白质级分的供给源的乳或乳来源的原料,可以使用牛乳、人乳、山羊乳、羊乳等乳,可以直接使用这些乳,或者也可以使用这些乳的还元乳、脱脂乳、乳清等。
在投与本发明的骨吸收抑制剂时,可以将作为有效成分的具有骨吸收抑制作用的乳蛋白质级分或乳蛋白质级分分解物以其原本的状态使用,也可以根据常规方法制剂为粉末剂、颗粒剂、片剂、胶囊剂、饮用剂等而使用。此外,还可以将这些乳蛋白质级分或乳蛋白质级分分解物直接或者制剂化后,将其配合到营养剂或饮食品等中,从而谋求抑制骨吸收。另外,本发明的乳蛋白质级分或乳蛋白质级分分解物对于热比较稳定,因而还能够在通常进行的加热杀菌条件下加热杀菌。
本发明中,为了发挥骨吸收抑制作用,可以考虑体重、性别和年龄等而适当确定配合量,通常在成人的情况下,可以将本发明品即乳蛋白质级分或乳蛋白质级分分解物的配合量等调整为每天摄取1~50mg。这样,本发明品在低用量下也具有效果。通过经口摄取将本发明的具有骨吸收抑制作用的成分制成骨吸收抑制剂、或者配合有其的药品、饮食品和饲料,从而发挥骨吸收抑制作用。
以下,示出参考例、实施例和试验例来详细说明本发明,但它们仅仅例示出本发明的实施方式,本发明并不受这些例子的任何限制。
[参考例1]
根据以下方法(参照日本专利第3112637号),调制可确认骨强化效果的市售乳蛋白质级分。
用去离子水充分清洗填充有0.5升作为阳离子交换树脂的磺化Chitopearl(富士纺绩制)的直径10cm的柱。以流速100ml/min使50升未杀菌脱脂乳通过该柱后,用去离子水充分清洗该柱,使含有0.95M氯化钠的0.05M磷酸缓冲液(pH7.0)2.5升通过该柱从而使吸附于树脂上的蛋白质洗脱。然后,通过反渗透(RO)膜处理对该洗脱液脱盐、浓缩后,进行冷冻干燥,获得粉末状的乳蛋白质级分。重复该操作2次,获得104g的蛋白质级分。该蛋白质级分的等电点为7.0~8.5,该蛋白质级分中所含的碱性氨基酸为17.8%。
实施例1
用去离子水充分清洗填充有0.5升作为阳离子交换树脂的磺化Chitopearl(富士纺绩制)的直径10cm的柱。以流速100ml/min使50升未杀菌脱脂乳通过该柱后,用含有0.3M氯化钠的0.05M磷酸缓冲液(pH7.0)充分清洗该柱,然后,使含有0.55M氯化钠的0.05M磷酸缓冲液(pH7.0)2.5升通过该柱从而使吸附于树脂上的蛋白质洗脱。然后,通过反渗透(RO)膜处理对该洗脱液脱盐、浓缩后,进行冷冻干燥,获得粉末状的乳蛋白质级分。进行5次该操作,获得37.4g的乳蛋白质级分。该级分的分子量为12,000~16,000道尔顿,等电点为10以上,该乳蛋白质级分中所含的构成氨基酸中,碱性氨基酸为18~20%。另外,碱性氨基酸/酸性氨基酸的比为0.7~0.9。
实施例2
用去离子水充分清洗填充有0.5升作为阳离子交换树脂的磺化Chitopearl(富士纺绩制)的直径10cm的柱。以流速100ml/min使50升未杀菌脱脂乳通过该柱后,用含有0.4M氯化钠的0.05M磷酸缓冲液(pH7.0)充分清洗该柱,使含有0.65M氯化钠的0.05M磷酸缓冲液(pH7.0)2.5升通过该柱从而使吸附于树脂上的蛋白质洗脱。然后,通过反渗透(RO)膜处理对该洗脱液脱盐、浓缩后,进行冷冻干燥,获得粉末状的乳蛋白质级分。进行3次该操作,获得22.8g的乳蛋白质级分。该级分的分子量为12,000~16,000道尔顿,等电点为10以上,该乳蛋白质级分中所含的构成氨基酸中,碱性氨基酸为18~20%。另外,碱性氨基酸/酸性氨基酸的比为0.7~0.9。
实施例3
将实施例1中得到的乳蛋白质级分37.4g溶解于10升蒸馏水中后,以2%的浓度添加胃蛋白酶(关东化学制),在37℃下边搅拌1小时边进行水解。接着,用氢氧化钠溶液中和至pH6.8后,添加1%胰酶(Sigma公司制),在37℃下反应2小时。反应后,在80℃下加热处理10分钟而使酶失活,然后获得乳蛋白质级分分解物36.2g。
实施例4
将实施例2中得到的乳蛋白质级分22.8g溶解于8升蒸馏水中后,以2%的浓度添加胰蛋白酶(关东化学制),在37℃下边搅拌1小时边进行水解。接着,用氢氧化钠溶液中和至pH6.6后,添加1%胰酶(Sigma公司制),在37℃下反应2小时。反应后,在80℃下加热处理10分钟而使酶失活,然后获得乳蛋白质级分分解物21.1g。
[试验例1]
关于参考例1和实施例1中得到的各乳蛋白质级分,按照高田等的方法[Takada,Y.et al.,Bone and Mineral,vol.17,pp.347-359,1992]对骨吸收抑制作用进行了研究。将出生后10~20天的ICR系小鼠的长骨摘出,除去软组织后,将骨在含5%胎牛血清的α-MEM溶液中机械性粉碎,得到包含破骨细胞的全部骨髓细胞。将该细胞通过含5%胎牛血清的α-MEM溶液在象牙片上点出约2×106个细胞。数小时后,加入添加了试验试样的含5%牛胎血清的α-MEM溶液,培养5天,研究存活的破骨细胞的骨吸收活性。另外,骨吸收的评价通过如下方式来进行,即培养后,剥离象牙片上的细胞,用苏木精染色,通过图像解析来测定骨吸收窝的面积。以试样空白作为对照,以对照的骨吸收窝的面积为100%时的各试样的面积%来表示骨吸收活性。其结果如表1所示。
表1
可确认:与对照组和参考例1中得到的乳蛋白质级分相比,本发明的乳蛋白质级分(实施例1)具有显著的骨吸收抑制作用。
[试验例2]
关于参考例1和实施例1中得到的各乳蛋白质级分,通过动物实验研究了骨吸收抑制作用。动物实验中使用了6周龄的Wistar系雌性大鼠。在1周的预备饲育后,实施卵巢摘除手术,然后以低钙饲料(钙含量0.3%)饲育5周,处于恢复期间,然后,供于动物实验。另外,摘除了卵巢、并以低钙饲料(AIN-76base)饲育了5周的大鼠明显处于骨质疏松症的状态。将产生了该骨质疏松症状态的大鼠以1组8只分别分配到下述3个试验组:未添加乳蛋白质级分的对照组(A组)、添加了0.1重量%的参考例1中得到的乳蛋白质级分的投与组(B组)、添加了0.1重量%的实施例1中得到的乳蛋白质级分的投与组(C组),分别用表2所示的试验饲料饲育4个月。另外,用酪蛋白调整各试验饲料的氮含量,使其具有相同的氮含量(17.06%)。
表2
对于饲育了4个月的大鼠,在最后的1周,用代谢笼回收尿,用osteolinks“DPD”(Sumitomo Seiyaku Biomedical Co.,Ltd.制造)测定尿中存在的骨吸收标志即脱氧吡啶啉(DPD)量(尿中总量)。此外,当用每天的排泄量不依赖于尿量的肌酸酐量来修正上述DPD浓度时,能够算出作为不依赖于尿量的骨吸收标志的更准确的DPD量。这里,尿中的肌酸酐浓度用Creatinine-testWako(和光纯药工业公司制造)进行测定,通过算出肌酸酐对应的DPD浓度而进行了修正。投与4个月后的尿中骨吸收标志即脱氧吡啶啉(DPD)的尿中总量和肌酸酐对应的DPD浓度的测定结果,如图1所示。由此可知,在1周的DPD总量和肌酸酐修正后的DPD浓度的任一者方面,与对照组(A组:未添加乳蛋白质级分)相比,参考例1中得到的乳蛋白质级分投与组(B组)、实施例1中得到的乳蛋白质级分投与组(C组)均表现出较低的值。虽然参考例1中得到的乳蛋白质也确认出具有骨吸收抑制效果,但本发明品的该作用明显更强。
另外,虽然没有示出实验结果,但在使用了实施例3和实施例4中得到的乳蛋白质级分分解物的情况下也确认到了同样的效果。
实施例5
在100mg实施例1中得到的乳蛋白质级分中添加93.4g水合晶体葡萄糖、5g碳酸钙、1g糖酯、0.5g香料,混和,然后压片制成片状,制造本发明的骨吸收抑制剂。
实施例6
以表3所示的组成混合各成分并制成生面团,成形后进行烘焙,制造骨吸收抑制用饼干。
表3
小麦粉 50.0(重量%)
砂糖 20.0
食盐 0.5
人造黄油 12.5
蛋 12.1
水 4.0
碳酸氢钠 0.1
碳酸氢铵 0.2
碳酸钙 0.5
乳蛋白质级分粉末(实施例1) 0.1
实施例7
制造表4所示的组成的骨吸收抑制用果汁饮料。
表4
混合异构化糖 15.0(重量%)
果汁 10.0
柠檬酸 0.5
乳蛋白质级分粉末(实施例1) 0.5
香料 0.1
钙 0.1
水 73.8
实施例8
根据表5所示的配方混合原料,制造骨吸收抑制用狗饲育饲料。
表5
乳蛋白质级分粉末(实施例1) 2.5(重量%)
脱脂乳粉 13.5
豆饼 12.0
豆油 4.0
玉米油 2.0
棕榈油 27.0
玉米淀粉 14.0
小麦粉 9.0
麸皮 2.0
维生素混合物 9.0
矿物质混合物 2.0
纤维素 3.0
实施例9
以表6所示的组成混合各成分,加压成形,调制配合有实施例3中得到的乳蛋白质级分分解物的骨吸收抑制用片剂。
表6
水合晶体葡萄糖 59.4(重量%)
乳蛋白质级分分解物(实施例3) 16.0
玉米淀粉 12.0
纤维素 4.0
玉米油 4.0
维生素混合物(含胆碱) 1.0
矿物质混合物 3.6
实施例10
以表7所示的组成混合各成分,在乳化温度85℃下乳化,调制配合有实施例4中得到的乳蛋白质级分分解物的骨吸收抑制用加工乳酪。
表7
高达干酪 43.0(重量%)
切达干酪 43.0
柠檬酸钠 2.0
乳蛋白质级分分解物(实施例4) 0.5
乳来源的钙 1.0
水 10.5
产业上的可利用性
本发明的乳蛋白质级分或乳蛋白质级分分解物由于具有骨吸收抑制作用,而且对于抑制骨质疏松症等骨量减少有效,因此,能够制成以具有骨吸收抑制作用的乳蛋白质级分或乳蛋白质级分分解物为有效成分的骨吸收抑制剂、以及配合有具有骨吸收抑制作用的乳蛋白质级分或乳蛋白质级分分解物的骨吸收抑制用药品、饮食品和饲料使用。
Claims (6)
1.一种乳蛋白质级分,其具有以下(1)~(4)的特性:
(1)其为乳来源;
(2)其为含有在十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)中分子量在12,000~16,000道尔顿范围内的蛋白质的级分;
(3)构成氨基酸的组成中含有18~20重量%的碱性氨基酸、且碱性氨基酸/酸性氨基酸的比在0.7~0.9的范围内;
(4)具有骨吸收抑制作用。
2.一种乳蛋白质级分分解物,其通过用蛋白酶将权利要求1所述的乳蛋白质级分分解而获得。
3.一种骨吸收抑制剂,其含有权利要求1所述的乳蛋白质级分或权利要求2所述的乳蛋白质级分分解物。
4.一种骨吸收抑制用药品,其配合有权利要求1所述的乳蛋白质级分或权利要求2所述的乳蛋白质级分分解物。
5.一种骨吸收抑制用饮食品,其配合有权利要求1所述的乳蛋白质级分或权利要求2所述的乳蛋白质级分分解物。
6.一种骨吸收抑制用饲料,其配合有权利要求1所述的乳蛋白质级分或权利要求2所述的乳蛋白质级分分解物。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2007-285408 | 2007-11-01 | ||
JP2007285408 | 2007-11-01 | ||
PCT/JP2008/003066 WO2009057282A1 (ja) | 2007-11-01 | 2008-10-28 | 骨吸収抑制用食品素材 |
Publications (1)
Publication Number | Publication Date |
---|---|
CN101868475A true CN101868475A (zh) | 2010-10-20 |
Family
ID=40590689
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN200880114098A Pending CN101868475A (zh) | 2007-11-01 | 2008-10-28 | 骨吸收抑制用食品原材料 |
Country Status (9)
Country | Link |
---|---|
US (1) | US8580740B2 (zh) |
EP (1) | EP2208733A4 (zh) |
JP (1) | JPWO2009057282A1 (zh) |
KR (1) | KR101441728B1 (zh) |
CN (1) | CN101868475A (zh) |
AU (1) | AU2008320272B2 (zh) |
CA (1) | CA2704042C (zh) |
NZ (1) | NZ585093A (zh) |
WO (1) | WO2009057282A1 (zh) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2210507B1 (en) * | 2007-11-01 | 2015-09-09 | Megmilk Snow Brand Co., Ltd. | Bone-strengthening compositions |
JP5783484B2 (ja) * | 2010-09-30 | 2015-09-24 | 雪印メグミルク株式会社 | 皮膚コラーゲン産生促進剤 |
JP5969225B2 (ja) * | 2012-03-09 | 2016-08-17 | 雪印メグミルク株式会社 | 骨強化剤 |
Family Cites Families (30)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
BE901672A (fr) | 1985-02-07 | 1985-08-07 | Oleofina Sa | Procede de purification de proteines du lait et de ses derives. |
JPH0725800B2 (ja) | 1987-04-10 | 1995-03-22 | 雪印乳業株式会社 | 硫酸エステル化物を用いて乳からラクトフエリンを分離、精製する方法 |
FR2616628B1 (fr) * | 1987-06-19 | 1989-09-29 | Entremont Sa | Procede pour extraire des proteines du lactoserum par adsorption et elution |
JP3089636B2 (ja) | 1989-09-05 | 2000-09-18 | ミノルタ株式会社 | 画像形成装置 |
JPH03112637A (ja) | 1989-09-27 | 1991-05-14 | Hitachi Chem Co Ltd | 接着剤付き化粧板及びその製造方法 |
JPH03160862A (ja) | 1989-11-17 | 1991-07-10 | Fujitsu Ltd | 予約情報確認方式 |
JP3160862B2 (ja) | 1990-11-15 | 2001-04-25 | 雪印乳業株式会社 | 骨強化食品、飼料及び医薬 |
DK0573668T3 (da) * | 1991-12-26 | 1998-04-27 | Snow Brand Milk Products Co Ltd | Knogleforstærkende faktor og levnedsmidler og drikkevarer indeholdende samme faktor |
JP3604159B2 (ja) | 1992-05-07 | 2004-12-22 | 雪印乳業株式会社 | 乳清由来の骨芽細胞増殖促進及び骨強化因子ならびに該因子を含有せしめた骨強化食品、医薬および飼料 |
JP3092874B2 (ja) | 1991-12-26 | 2000-09-25 | 雪印乳業株式会社 | 乳清由来の骨芽細胞増殖・骨強化画分及び該画分を含有する骨強化食品、飼料、薬品類 |
JPH05202098A (ja) | 1992-01-29 | 1993-08-10 | Snow Brand Milk Prod Co Ltd | 乳質原料から生理活性物質の製造法 |
JP3460851B2 (ja) * | 1993-04-28 | 2003-10-27 | 雪印乳業株式会社 | 新規システインプロテアーゼインヒビター |
JP3112637B2 (ja) * | 1994-09-30 | 2000-11-27 | 雪印乳業株式会社 | 骨強化剤 |
JP2974604B2 (ja) * | 1996-01-23 | 1999-11-10 | 雪印乳業株式会社 | 塩基性タンパク質組成物、塩基性ペプチド組成物及びその利用 |
JP3018313B2 (ja) * | 1996-02-23 | 2000-03-13 | 雪印乳業株式会社 | 骨形成促進及び骨吸収防止剤 |
JP3929088B2 (ja) * | 1996-06-20 | 2007-06-13 | 雪印乳業株式会社 | 骨形成促進及び骨吸収防止剤 |
JP4082782B2 (ja) * | 1998-04-30 | 2008-04-30 | 雪印乳業株式会社 | 歯周病予防及び改善剤 |
JP4592127B2 (ja) * | 1999-03-30 | 2010-12-01 | 雪印乳業株式会社 | 骨吸収抑制剤 |
JP2001346519A (ja) | 2000-06-09 | 2001-12-18 | Snow Brand Milk Prod Co Ltd | 乳塩基性シスタチン高含有画分及びその分解物の製造方法 |
AU784087B2 (en) * | 2000-06-09 | 2006-02-02 | Megmilk Snow Brand Co., Ltd. | Method of producing fractions containing a high concentration of milk basic cystatin and decomposition products thereof |
US6649590B2 (en) * | 2000-06-09 | 2003-11-18 | Snow Brand Milk Products Co., Ltd. | Method of producing fractions containing a high concentration of milk basic cystatin and decomposition products thereof |
JP2004115509A (ja) * | 2002-09-05 | 2004-04-15 | Snow Brand Milk Prod Co Ltd | 破骨細胞分化抑制因子産生促進剤 |
JP4698935B2 (ja) * | 2003-05-07 | 2011-06-08 | 雪印乳業株式会社 | 皮膚コラーゲン産生促進剤 |
JP2004331556A (ja) * | 2003-05-07 | 2004-11-25 | Japan Science & Technology Agency | 2”−ヒドロキシニコチアナミン、その製造方法、並びにアンジオテンシン変換酵素阻害剤、血圧降下剤及び健康食品 |
DE60335665D1 (de) * | 2003-05-07 | 2011-02-17 | Snow Brand Milk Products Co Ltd | Mittel zur förderung der hautkollagenproduktion |
JP4536341B2 (ja) | 2003-08-15 | 2010-09-01 | 雪印乳業株式会社 | 骨形成促進剤 |
KR101303371B1 (ko) | 2005-08-30 | 2013-09-03 | 코닌클리즈케 필립스 일렉트로닉스 엔.브이. | 소정 범위의 속도들에 걸친 객체와 광 입력 장치의 상대이동을 측정하는 방법 |
WO2008143335A1 (en) | 2007-05-21 | 2008-11-27 | Canon Kabushiki Kaisha | Method for producing polymerized toner, polymerized toner, method for producing binder resin for toner and binder resin for toner |
DK2208734T3 (da) * | 2007-11-01 | 2012-05-14 | Megmilk Snow Brand Co Ltd | Levnedsmiddelmateriale til inhibering af dannelsen af osteoclast |
JP5783484B2 (ja) * | 2010-09-30 | 2015-09-24 | 雪印メグミルク株式会社 | 皮膚コラーゲン産生促進剤 |
-
2008
- 2008-10-28 WO PCT/JP2008/003066 patent/WO2009057282A1/ja active Application Filing
- 2008-10-28 EP EP08843536.7A patent/EP2208733A4/en not_active Withdrawn
- 2008-10-28 US US12/740,711 patent/US8580740B2/en active Active
- 2008-10-28 NZ NZ585093A patent/NZ585093A/xx unknown
- 2008-10-28 CA CA2704042A patent/CA2704042C/en active Active
- 2008-10-28 JP JP2009538921A patent/JPWO2009057282A1/ja active Pending
- 2008-10-28 AU AU2008320272A patent/AU2008320272B2/en active Active
- 2008-10-28 CN CN200880114098A patent/CN101868475A/zh active Pending
- 2008-10-28 KR KR1020107012033A patent/KR101441728B1/ko active IP Right Grant
Also Published As
Publication number | Publication date |
---|---|
NZ585093A (en) | 2013-03-28 |
KR101441728B1 (ko) | 2014-09-17 |
WO2009057282A1 (ja) | 2009-05-07 |
CA2704042C (en) | 2017-06-20 |
EP2208733A1 (en) | 2010-07-21 |
AU2008320272A1 (en) | 2009-05-07 |
JPWO2009057282A1 (ja) | 2011-03-10 |
CA2704042A1 (en) | 2009-05-07 |
AU2008320272B2 (en) | 2014-01-23 |
US20100261883A1 (en) | 2010-10-14 |
EP2208733A4 (en) | 2014-08-06 |
KR20100105561A (ko) | 2010-09-29 |
US8580740B2 (en) | 2013-11-12 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US20120040908A1 (en) | Fracture repair promoter | |
CN101842385B (zh) | 破骨细胞形成抑制用食品原材料 | |
JPH04183371A (ja) | 骨強化食品、飼料及び医薬 | |
CN101861332B (zh) | 用于促进成骨细胞分化和抑制破骨细胞分化的食品原材料 | |
CN101842026B (zh) | 骨强化用食品原材料 | |
CN101868475A (zh) | 骨吸收抑制用食品原材料 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C41 | Transfer of patent application or patent right or utility model | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20110907 Address after: Hokkaido Japan Applicant after: Megmilk Snow Brand Co., Ltd. Address before: Hokkaido Japan Applicant before: Snow Brand Milk Products Co., Ltd. |
|
C12 | Rejection of a patent application after its publication | ||
RJ01 | Rejection of invention patent application after publication |
Application publication date: 20101020 |