CN101785420A - Heeling in technology of tissue culture seedlings of bananas - Google Patents
Heeling in technology of tissue culture seedlings of bananas Download PDFInfo
- Publication number
- CN101785420A CN101785420A CN200910002890A CN200910002890A CN101785420A CN 101785420 A CN101785420 A CN 101785420A CN 200910002890 A CN200910002890 A CN 200910002890A CN 200910002890 A CN200910002890 A CN 200910002890A CN 101785420 A CN101785420 A CN 101785420A
- Authority
- CN
- China
- Prior art keywords
- bananas
- tissue culture
- culture
- seedling
- technology
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Abstract
The invention discloses soilless culture technology and in particular relates to soilless heeling in technology of tissue culture seedlings of bananas. The method is characterized by mixing organic substrates and inorganic substrates in a volume ratio of 1:1 as the culture substrates and applying inorganic N, P and K fertilizers in a ratio of 1:0.4:3.5 to prepare aqueous solution at the concentration of 0.1-0.3% to culture the tissue culture seedlings of bananas. The culture substrates of the invention have good permeability and can better preserve moisture and fertility, thus avoiding infection harm caused by the viruses and pests carried by soil; compared with the soil culture, the culture substrates are more beneficial to root system growth of the tissue culture seedlings of bananas and ensure the survival rate of the tissue culture seedlings to be as high as 94-100%; the tissue culture seedlings of bananas grow and develop robust; in addition, the culture substrates of the invention are easy to detach from the pot compared with the traditional soil culture, thus being beneficial to recycle of nutrition pots and environmental protection.
Description
Technical field
The present invention relates to a kind of cultivation technique without soil, specifically, relate to a kind of no native heeling in technology of tissue culture seedlings of bananas.
Background technology
At present, soil cultivation is all adopted in heeling in of tissue culture seedlings of bananas, and this is most economical effective cultivation step, but portable virus of soil and insect, tissue culture seedlings of bananas is heeled in infecting of phase susceptible viral and insect, and soil is heavier, for transplanting land for growing field crops, nutrient cup recycling inconvenience is arranged.
Summary of the invention
A kind of heeling in technology of tissue culture seedlings of bananas that the present invention develops in order to solve the problems of the technologies described above is just heeled in the phase and is subjected to damage by disease and insect to infect probability to reduce tissue culture seedlings of bananas, and reduces and transplant cost.
The technical solution adopted for the present invention to solve the technical problems is:
A kind of heeling in technology of tissue culture seedlings of bananas, cultivation matrix are that organic substrate and inorganic matrix mixed in 1: 1 by volume; Nutrient solution is an inorganic fertilizer, preferred N, P, the fertile aqueous solution that is mixed with concentration 0.1%～0.3% in 1: 0.4: 3.5 ratio of K; Its cultivation method is as follows:
1. organic substrate, inorganic matrix were mixed as cultivation matrix in 1: 1 by volume; Add 40 kilograms of fertilizers in every cubic metre of cultivation matrix during mixing;
2. in the nutrient cup of the matrix that mixes being packed into;
3. transplantation rooting bag seedling: bag medium that shoot root portion the attaches clear water wash clean of taking root that will be after hardening, soaked any of several broadleaf plants shoot root portion 1 minute with 600 times of liquid of carbendazim; After seedling carried out classification, divide furrow to transplant the seedling of differing heights, thoroughly irrigate matrix in order to normal root, put into little shed, on little shed, add a cover plastic film then with free of contamination clean water;
4. transplant in 7 days, see dried watering, throw off the little shed of plastic film after 7 days;
5. after treating that seedling begins to extract out first young leaves, begin to execute nutrient solution, weekly twice, concentration is 0.1%;
The complete paleness of seedling is green after 10 days, and nutrient solution concentration is enlarged to 0.2～0.3%;
6. emerging stopped fertilising in preceding 10 days, cooperated control water and control light to reach the hardening requirement.
Described heeling in technology of tissue culture seedlings of bananas, the preferred coconut palm chaff of its organic substrate, mushroom slag, peat, wood chip or its mixture.
Described heeling in technology of tissue culture seedlings of bananas, the preferred perlite of its inorganic matrix, vermiculite, river sand or its mixture.
Described heeling in technology of tissue culture seedlings of bananas, it is executed the nutrient solution arrangement of time and carries out when temperature is low after 4 o'clock in the afternoon, executes that morning of nutrient solution nutrient cup is drenched with clear water, and m seq drenches clear water washes leaf, with unburned seedling.
The invention has the beneficial effects as follows: space is preferably arranged between the cultivation matrix particle, granule interior has flourishing hole and micropore, this hole and micropore have permeability preferably, and have to absorb and store and discharge the grow characteristic of required liquid manure of tissue culture seedlings of bananas, preserve moisture and fertility preferably, liquid manure are difficult for infiltration and run off, the fertilizer of can economizing on water, be more conducive to the root growth of tissue culture seedlings of bananas than soil cultivation, survival rate is up to 94%～100%; This cultivation matrix can avoid since the virus brought of soil and insect infect harm, overcome disadvantages such as the prior art nematode multiplies, Panama disease spreads, tissue culture seedlings of bananas is heeled in the stalwartness of growing, and transplants the land for growing field crops survival rate up to 95～100%; In addition, because cultivation matrix of the present invention is easier to take off cup compared to traditional soil cultivation, help the recycling and the environmental protection of nutrient cup; Because mixed culture matrix weight is lighter, be 1/2～1/4 of normal soil only, be used for tissue culture seedlings of bananas and heel in also and can reduce cost of transportation.
Description of drawings
Fig. 1 is a heeling in technology of tissue culture seedlings of bananas implementation step flow chart of the present invention.
Embodiment
The present invention is further described below in conjunction with drawings and Examples.
Embodiment one, and its step as shown in Figure 1.
(1) cool canopy construction: build highly 2 meters, the top is the cool canopy of flat roof type, covers degree of shading and be 75% sunshade net, fixes with iron wire.
(2) seedling: use bag seedling of taking root after 5 days outdoor hardenings.
(3) mixed culture matrix: with the mixing of 1: 1 ratio (volume ratio), every cubic metre of matrix adds 40 kilograms of fertilizers during mixing with coconut palm chaff, river sand.
(4) dress matrix: the matrix that mixes is packed in 10cm * 10cm nutrient cup.
(5) transplantation rooting bag seedling: bag medium that shoot root portion the attaches clear water wash clean of taking root that will be after hardening, soaked any of several broadleaf plants shoot root portion 1 minute with 600 times of liquid of carbendazim.After seedling carried out classification, divide furrow to transplant the seedling of differing heights, add a cover the little shed of plastic film after watering sufficient normal root water.
(6) transplant in 7 days, see dried watering, throw off the little shed of plastic film after 7 days.After treating that seedling begins to extract out first young leaves, begin to execute nutrient solution, twice weekly, concentration is that the complete paleness of seedling is green after 0.1%, 10 day, and nutrient solution concentration is enlarged to 0.2～0.3%, fertilization time generally should be arranged in when temperature is low after at 4 o'clock in afternoon and carry out, the that morning of using is drenched the nutrient cup water, and the m seq trickle " is washed leaf ", with unburned seedling.Emerging stopped fertilising in preceding 10 days, cooperated control water and control light to reach the hardening requirement.
(7) in whole seedling raising process, must in time accomplish large, medium and small seedling Classification Management, require branch to put when dividing seedling, manage by large, medium and small three kinds of specifications classification.
(8) extermination of disease and insect pest: leaf is protected in diseases prevention in time after under the seed, leaf spot and cauline leaf soft rot when main, and the number of blade of plant must not be lower than 5 leaves when going out the garden.Owing to adopt soilless culture substrate, there is not soil insect harm seedling stage, medication mostly is sterilization class medicine, and spray date generally should be arranged in before at 10 in the morning.
The present invention is not limited to above-mentioned preferred forms, and other any identical with the present invention or akin products that anyone draws under enlightenment of the present invention all drop within protection scope of the present invention.
Claims (4)
1. heeling in technology of tissue culture seedlings of bananas, it is characterized in that: cultivation matrix is that organic substrate and inorganic matrix mixed in 1: 1 by volume; Nutrient solution is an inorganic fertilizer, and preferred N, P, K are fertile to be mixed with the aqueous solution of concentration 0.1%～0.3% in 1: 0.4: 3.5 ratio, and its cultivation method is as follows:
1. organic substrate, inorganic matrix were mixed as cultivation matrix in 1: 1 by volume; Add 40 kilograms of fertilizers in every cubic metre of cultivation matrix during mixing;
2. in the nutrient cup of the matrix that mixes being packed into;
3. transplantation rooting bag seedling: bag medium that shoot root portion the attaches clear water wash clean of taking root that will be after hardening, soaked any of several broadleaf plants shoot root portion 1 minute with 600 times of liquid of carbendazim; After seedling carried out classification, divide furrow to transplant the seedling of differing heights, thoroughly irrigate matrix in order to normal root, put into little shed, on little shed, add a cover plastic film then with free of contamination clean water;
4. transplant in 7 days, see dried watering, throw off the little shed of plastic film after 7 days;
5. after treating that seedling begins to extract out first young leaves, begin to execute nutrient solution, weekly twice, concentration is 0.1%; The complete paleness of seedling is green after 10 days, and nutrient solution concentration is enlarged to 0.2～0.3%;
6. emerging stopped fertilising in preceding 10 days, cooperated control water and control light to reach the hardening requirement.
2. heeling in technology of tissue culture seedlings of bananas according to claim 1 is characterized in that: the preferred coconut palm chaff of organic substrate, mushroom slag, peat, wood chip or its mixture.
3. heeling in technology of tissue culture seedlings of bananas according to claim 1 is characterized in that: the preferred perlite of inorganic matrix, vermiculite, river sand or its mixture.
4. heeling in technology of tissue culture seedlings of bananas according to claim 1, it is characterized in that: execute the nutrient solution arrangement of time and carry out when temperature is low after 4 o'clock in the afternoon, execute that morning of nutrient solution nutrient cup is drenched with clear water, m seq drenches clear water washes leaf, with unburned seedling.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910002890A CN101785420A (en) | 2009-01-22 | 2009-01-22 | Heeling in technology of tissue culture seedlings of bananas |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910002890A CN101785420A (en) | 2009-01-22 | 2009-01-22 | Heeling in technology of tissue culture seedlings of bananas |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101785420A true CN101785420A (en) | 2010-07-28 |
Family
ID=42528924
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200910002890A Pending CN101785420A (en) | 2009-01-22 | 2009-01-22 | Heeling in technology of tissue culture seedlings of bananas |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101785420A (en) |
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102077779A (en) * | 2010-10-27 | 2011-06-01 | 广东省农业科学院蔬菜研究所 | Vegetable seedling culture soilless matrix compounded with straw mushroom and preparation method thereof |
| CN102613053A (en) * | 2012-04-05 | 2012-08-01 | 中国热带农业科学院环境与植物保护研究所 | Water culture method for banana tissue-culture seedlings |
| CN102657039A (en) * | 2012-05-30 | 2012-09-12 | 中国热带农业科学院海口实验站 | Detection method for growth state of roots of banana temporarily-planted seedlings |
| CN102845287A (en) * | 2012-09-04 | 2013-01-02 | 中国热带农业科学院热带生物技术研究所 | Water-culture method and system of banana seedlings |
| CN103960018A (en) * | 2014-05-14 | 2014-08-06 | 中国热带农业科学院海口实验站 | Method for increasing survival rate of transplanting banana temporarily-planted seedlings to big field |
| CN104429858A (en) * | 2014-12-12 | 2015-03-25 | 华南农业大学 | Culture substrate for quick cultivation of banana heeled-in seedlings and preparing method thereof |
| CN105145237A (en) * | 2015-07-31 | 2015-12-16 | 王振华 | Plantation method for bananas |
| CN105660158A (en) * | 2016-03-22 | 2016-06-15 | 东莞市金麻实业投资有限公司 | Antibacterial and insect-resisting cultivation method for bananas |
| CN107162673A (en) * | 2017-04-26 | 2017-09-15 | 闽南师范大学 | A kind of cultivation matrix of quickly breeding Banana Seedlings |
| CN109122316A (en) * | 2018-08-28 | 2019-01-04 | 佛山科学技术学院 | A method of it improving the anti-silver ion of tissue culture seedlings of bananas and coerces ability |
| CN111149634A (en) * | 2020-03-03 | 2020-05-15 | 广西壮族自治区农业科学院 | Cultivation method for interplanting bananas and black-skinned wax gourds |
| CN111670779A (en) * | 2020-05-29 | 2020-09-18 | 广东省农业科学院农业资源与环境研究所 | Banana tissue culture seedling improvement matrix and preparation method and application thereof |
| CN113455319A (en) * | 2021-06-21 | 2021-10-01 | 百色学院 | Transplanting method of cassava tissue culture contaminated seedlings |
| CN114402876A (en) * | 2022-01-27 | 2022-04-29 | 广西壮族自治区农业科学院 | Green pest control method for banana in greenhouse seedling stage |
-
2009
- 2009-01-22 CN CN200910002890A patent/CN101785420A/en active Pending
Cited By (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102077779A (en) * | 2010-10-27 | 2011-06-01 | 广东省农业科学院蔬菜研究所 | Vegetable seedling culture soilless matrix compounded with straw mushroom and preparation method thereof |
| CN102613053A (en) * | 2012-04-05 | 2012-08-01 | 中国热带农业科学院环境与植物保护研究所 | Water culture method for banana tissue-culture seedlings |
| CN102613053B (en) * | 2012-04-05 | 2013-05-01 | 中国热带农业科学院环境与植物保护研究所 | Water culture method for banana tissue-culture seedlings |
| CN102657039A (en) * | 2012-05-30 | 2012-09-12 | 中国热带农业科学院海口实验站 | Detection method for growth state of roots of banana temporarily-planted seedlings |
| CN102845287A (en) * | 2012-09-04 | 2013-01-02 | 中国热带农业科学院热带生物技术研究所 | Water-culture method and system of banana seedlings |
| CN103960018A (en) * | 2014-05-14 | 2014-08-06 | 中国热带农业科学院海口实验站 | Method for increasing survival rate of transplanting banana temporarily-planted seedlings to big field |
| CN104429858A (en) * | 2014-12-12 | 2015-03-25 | 华南农业大学 | Culture substrate for quick cultivation of banana heeled-in seedlings and preparing method thereof |
| CN104429858B (en) * | 2014-12-12 | 2017-07-07 | 中海石油化学股份有限公司 | A kind of cultivation matrix of quickly breeding Banana Seedlings and preparation method thereof |
| CN105145237B (en) * | 2015-07-31 | 2018-06-19 | 宁波远志立方能源科技有限公司 | A kind of implantation methods of banana |
| CN105145237A (en) * | 2015-07-31 | 2015-12-16 | 王振华 | Plantation method for bananas |
| CN105660158A (en) * | 2016-03-22 | 2016-06-15 | 东莞市金麻实业投资有限公司 | Antibacterial and insect-resisting cultivation method for bananas |
| CN107162673A (en) * | 2017-04-26 | 2017-09-15 | 闽南师范大学 | A kind of cultivation matrix of quickly breeding Banana Seedlings |
| CN109122316A (en) * | 2018-08-28 | 2019-01-04 | 佛山科学技术学院 | A method of it improving the anti-silver ion of tissue culture seedlings of bananas and coerces ability |
| CN111149634A (en) * | 2020-03-03 | 2020-05-15 | 广西壮族自治区农业科学院 | Cultivation method for interplanting bananas and black-skinned wax gourds |
| CN111670779A (en) * | 2020-05-29 | 2020-09-18 | 广东省农业科学院农业资源与环境研究所 | Banana tissue culture seedling improvement matrix and preparation method and application thereof |
| CN113455319A (en) * | 2021-06-21 | 2021-10-01 | 百色学院 | Transplanting method of cassava tissue culture contaminated seedlings |
| CN114402876A (en) * | 2022-01-27 | 2022-04-29 | 广西壮族自治区农业科学院 | Green pest control method for banana in greenhouse seedling stage |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101785420A (en) | Heeling in technology of tissue culture seedlings of bananas | |
| CN103798030B (en) | Anji white tea ecological and efficient cultivation method | |
| CN103460921B (en) | A kind of method of Emmenopterys henryi non-polluted planting | |
| CN102577816B (en) | Slope crop seeding raising method | |
| CN103299809B (en) | Organic vegetables multi-cropping potting technology | |
| CN1305368C (en) | Bowl culture of aerial orchidaceae | |
| CN102487686A (en) | Large-scale seedling-culturing method utilizing cassia sophera seeds and nutrient solution thereof | |
| CN103535169B (en) | Method for cultivating pigment marigolds in sand of high and cold region | |
| CN104904442A (en) | Method for planting bletillastriata under forest | |
| CN104823655A (en) | Cultivation method of mulberries | |
| CN103283445A (en) | Method for comprehensively applying biogas and biogas manure to seedling growth of flower and tree seeds | |
| CN103460941A (en) | Method for cultivating semi-annual high-rosin pinus massoniana lamb seedlings in nutrient bags | |
| CN105028103A (en) | Sunlight greenhouse trough cultivation method for peach plants | |
| CN103444395A (en) | Pollution-free cultivation production method for young narcissus balls | |
| CN104396521A (en) | Artificial seedling reproducing technique for huanghua pear trees | |
| CN103875423A (en) | Method for cultivating asparagus | |
| CN105075785A (en) | Two-stage cultivation method for oil camellia container seedlings | |
| CN104813838A (en) | Cultivating method capable of prolonging flowering phase of oriental cherry | |
| CN105850579A (en) | Succulent plant mushroom residue source substrate and preparation method thereof | |
| CN103583346A (en) | Soilless cultivation method for citrus bergamot | |
| CN102792831B (en) | High-efficiency rapid propagation technique for Chinese yew | |
| CN106069471A (en) | A kind of breeding method of nursery stock | |
| CN109744058A (en) | Mount Taishan sibirica seeds vernalization and method for culturing seedlings | |
| CN109122157A (en) | A kind of fertilizing method promoting live streaming seedling fast-growth in bletilla striata greenhouse | |
| CN105454013A (en) | Seedling cultivation medium of machilus versicolora, and seedling cultivation method by using same |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20100728 |