CN102613053B - Water culture method for banana tissue-culture seedlings - Google Patents
Water culture method for banana tissue-culture seedlings Download PDFInfo
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- CN102613053B CN102613053B CN2012100971228A CN201210097122A CN102613053B CN 102613053 B CN102613053 B CN 102613053B CN 2012100971228 A CN2012100971228 A CN 2012100971228A CN 201210097122 A CN201210097122 A CN 201210097122A CN 102613053 B CN102613053 B CN 102613053B
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Abstract
The invention relates to a water culture method for banana tissue-culture seedlings. The method comprises the following steps of: 1) performing rooting culture on the banana tissue-culture seedlings at the temperature of between 22 and 35DEG C for 10 to 15 days in a water-based rooting culture solution; and 2) replacing the water-based rooting culture solution by using a water-based nutrient solution, performing nutrient culture at the temperature of between 20 and 35DEG C for 20 to 30 days, cupping for raising the seedlings in a greenhouse, and producing in field. The water culture method for the banana tissue-culture seedlings is easy and convenient, high in production efficiency, small in floor area, and suitable for intensive pollution-free facility cultivation or farmer scattered cultivation, and avoids a three-waste problem. By the method, the banana tissue-culture seedlings are hardly infected by viruses, pathogenic bacteria and insect pests in the temporary planting period, and can be used for scientific research, such as research on the process that blight infects bananas.
Description
Technical field
The present invention relates to agriculture field, specifically, the present invention relates to a kind of method of water planting tissue culture seedlings of bananas.
Background technology
Banana (Musa cavendishii) belongs to the green perennial large-scale herbaceous plant of length of Musaceae, is one of tropical main fruit.At present, the banana factorial seedling growth has been widely used in production in many countries, starts from 20th century the mid-80 in China, and many areas such as Guangdong, Guangxi, Hainan are the setting up a factory commercial production system of growing seedlings in succession.Producing in recent years the upper banana seedling that adopts is group training seedling more than 90%, can provide technical support for banana industry production.
At present, the cultivation of tissue culture seedlings of bananas comprises mainly that husky bed is heeled in, raise seedling in greenhouse, goes out the garden three phases.Husky bed at tissue culture seedlings of bananas is heeled in the stage, because sandy soil, the portable virus of coconut palm chaff and insect are difficult to thorough disinfection, causes organizing the dip-dye that the training seedling is heeled in phase susceptible viral, pathogen and insect.
Summary of the invention
The method that the purpose of this invention is to provide a kind of water planting tissue culture seedlings of bananas.
In order to realize purpose of the present invention, the invention provides a kind of method of water planting tissue culture seedlings of bananas, the method comprises:
Step 1): make tissue culture seedlings of bananas in water base culture of rootage liquid, under 22 ℃ to 35 ℃, carry out culture of rootage 10 to 15 days; And
Step 2): use water base nutrient solution to replace described water base culture of rootage liquid, carry out nutrition and cultivated 20 to 30 days under 20 ℃ to 35 ℃, this moment, every young plant grew 5～7 young leaves, and natural height 15 is to 20cm.
Tradition tissue culture seedlings of bananas production and cultivating process comprise: 1, the production of tissue culture seedlings of bananas comprises that seed selection, the production of hybrid seeds, expansion are numerous, takes root and hardening; 2, the cultivation of tissue culture seedlings of bananas comprises that husky bed is heeled in, raise seedling in greenhouse, goes out garden (namely can carry out field planting).The method of water planting tissue culture seedlings of bananas of the present invention is used for replacing traditional husky bed to heel in the method for tissue culture seedlings of bananas.
As used in this article, term " tissue culture seedlings of bananas " refers to use tissue culture technology, optimizes Comprehensive Traits good from the banana variety of establishing in large scale, output is high, and adaptable fine individual plant is by detoxification, carry out cultivation strict, meticulous, science, guaranteed the excellent stability of kind.It has following advantages: 1, seedling is neat, and the speed of breeding is fast, and is large for the seedling amount.Determine the banana breeding selected, just can use tissue culture technology and carry out large-scale production, a large amount of height, blade, high quality seedling that thickness is consistent are provided in a short time, form commodity market, this is that traditional suction bud seedling production is beyond one's reach.2, growth is fast, and growing way is neat, and is ripe consistent.Simultaneously plantation is inhaled the bud seedling and is organized the training seedling, and group training seedling growing way is faster than inhaling the bud seedling, neatly, takes out in advance the flower bud maturation, and ripe consistent, gathers concentrated convenient management and sale.3, high yield and high quality, fruit shape is good, and commodity rate is high, storage tolerance, market price is high, and is generally high 0.2～0.3 yuan than the banana per kilogram of inhaling the production of bud seedling, welcome by numerous banana peasants and buyer agent.4, aseptic seedling.Strictly screening and the detoxification of banana group training original seed process, and definite virus-free through strict detection, just produce and breed, very strong premunition is arranged, can effectively resist disease.
At present, the commercialization of the production stage of tissue culture seedlings of bananas, the tissue culture seedlings of bananas before cultivating can commercially be bought and obtain, for example available from Chinese Academy of Tropical Agricultural Sciences seedling group training center.
As used in this article, term " water base " refers to the system take water as solvent.
Herein, the consumption of water base culture of rootage liquid and water base nutrient solution is not particularly limited, and those skilled in the art can judge concrete consumption according to conventional methods.
In step 1) in, for example can adopt PVC plate and tank is that cultivating container comes tissue culture seedlings of bananas is carried out culture of rootage.
Preferably, in described step 1) in, the prescription of described water base culture of rootage liquid is: contain in 1L water: contain in 1L water: potassium nitrate 500-520mg, ammonium nitrate 100-150mg, potassium dihydrogen phosphate 110-150mg, magnesium sulfate 230-250mg, calcium chloride 330-350mg, EDETATE SODIUM iron 20-40mg, boric acid 1.00-1.50mg, four hydrated manganese sulfate 2.00-2.50mg, Zinc vitriol 0.800-0.900mg, Salzburg vitriol 0.100-0.150mg, four molybdic acid hydrate 0.100-0.150mg, NAA (being also referred to as α-naphthaleneacetic acid) 0.1-0.5mg and 6-BA (being also referred to as 6-benzyl aminopurine) 0.1-0.5mg
More preferably, in described step 1) in, the prescription of described water base culture of rootage liquid is: contain in 1L water: potassium nitrate 505mg, ammonium nitrate 120mg, potassium dihydrogen phosphate 136mg, magnesium sulfate 246mg, calcium chloride 333mg, EDETATE SODIUM iron 24mg, boric acid 1.24mg, four hydrated manganese sulfate 2.23mg, Zinc vitriol 0.864mg, Salzburg vitriol 0.125mg, four molybdic acid hydrate 0.117mg, NAA 0.3mg and 6-BA 0.2mg.
Preferably, in described step 1) in, the spacing of described tissue culture seedlings of bananas is not less than 0.5cm.If spacing is less than 0.5cm, density is excessive, is unfavorable for the growth of tissue culture seedlings of bananas.And the false basal part of stem of described tissue culture seedlings of bananas is higher than the liquid level of described water base culture of rootage liquid, if be lower than the liquid level of described water base culture of rootage liquid, then easily causes the false stem rot of tissue culture seedlings of bananas mashed.
In described step 2) in, under 20 ℃-35 ℃, carry out nutrition and cultivated 20 to 30 days, and can not be exposed to the sun under sunlight, prevent that the liquid fever hinders root.Carry out step 2) time, use water base nutrient solution to replace described water base culture of rootage liquid, and container can not changed.
Preferably, in described step 2) in, the prescription of described water base nutrient solution is: contain in 1L water: potassium nitrate 300-530mg, ammonium nitrate 50-150mg, potassium dihydrogen phosphate 50-150mg, magnesium sulfate 100-250mg, calcium chloride 150-350mg, EDETATE SODIUM iron 10-40mg, boric acid 1.00-1.50mg, four hydrated manganese sulfate 1.00-2.50mg, Zinc vitriol 0.500-0.900mg, Salzburg vitriol 0.100-0.150mg and four molybdic acid hydrate 0.100-0.150mg.
More preferably, in described step 2) in, the prescription of described water base nutrient solution is: contain in 1L water: potassium nitrate 505mg, ammonium nitrate 120mg, potassium dihydrogen phosphate 136mg, magnesium sulfate 246mg, calcium chloride 333mg, EDETATE SODIUM iron 24mg, boric acid 1.24mg, four hydrated manganese sulfate 2.23mg, Zinc vitriol 0.864mg, Salzburg vitriol 0.125mg and four molybdic acid hydrate 0.117mg.
Preferably, described water base nutrient solution was changed once every 10-20 days.
Preferably, described water base nutrient solution circulated once every 1-2 days.As used herein, term " circulation " refers to circulate once in employed container and gets final product, and is not particularly limited for the shape of container, type, volume etc.For example, if when using tank, refer to circulate once in tank every 1-2 days.
Preferably, when supporting cultivation through the laggard field headquarters of culture of rootage, the liquid level of the described water base nutrient solution of the bulb of described tissue culture seedlings of bananas bottom contact when initial, after new root grew, described liquid level was down to 4/5 place of root.In this process, those skilled in the art can easily identify this process and carry out accommodation.
Preferably, every foliage fertilizer of spray in 5-7 days, described foliage fertilizer is the aqueous solution of 0.1 % by weight urea and 0.05 % by weight potassium dihydrogen phosphate.During sprinkling, be sprayed on the seedling equably and get final product.The sprinkling amount is not particularly limited, as long as can satisfy the seedling needed nutrition of growing.
The present invention replaces husky bed to heel in tissue culture seedlings of bananas by the water planting tissue culture seedlings of bananas, so that tissue culture seedlings of bananas is being heeled in the not dip-dye of susceptible viral, pathogen and insect of phase.The method production technology of water planting tissue culture seedlings of bananas of the present invention is easy, production efficiency is high, and floor space is little, is applicable to the scattered cultivation of intensive nuisanceless facility cultivation or peasant household and without three wastes problem.Heel in compared with the husky bed of tradition, have the following advantages: at first make tissue culture seedlings of bananas heel in the not dip-dye of susceptible viral, pathogen and insect of phase; Secondly, water planting group training seedling is heeled in the fast of group training seedling growth than husky bed; Can be used for scientific research at last, for example study the process that fusarium wilt infects banana with the water planting banana.
Embodiment
Following examples are used for explanation the present invention, but are not used for limiting the scope of the invention.
Embodiment 1 water planting tissue culture seedlings of bananas of the present invention
Adopting PVC plate and tank is cultivating container.Use clear water to wash tissue culture seedlings of bananas (Brazilian any of several broadleaf plants off, available from Chinese Academy of Tropical Agricultural Sciences seedling group training center), the tissue culture seedlings of bananas of wash clean is inserted on the PVC plate, and the seedling spacing is 1cm, and the false basal part of stem of described tissue culture seedlings of bananas is higher than the liquid level of described water base culture of rootage liquid.Add water base culture of rootage liquid in the tank, under 22 ℃, carried out culture of rootage 15 days.The prescription of described water base culture of rootage liquid is: contain in 1L water: potassium nitrate 500mg, ammonium nitrate 150mg, potassium dihydrogen phosphate 110mg, magnesium sulfate 250mg, calcium chloride 330mg, EDETATE SODIUM iron 20mg, boric acid 1.50mg, four hydrated manganese sulfate 2.00mg, Zinc vitriol 0.800mg, Salzburg vitriol 0.150mg, four molybdic acid hydrate 0.150mg, NAA 0.1mg and 6-BA 0.5mg.
Afterwards, use water base nutrient solution to replace described water base culture of rootage liquid, under 35 ℃, carry out nutrition and cultivated 30 days.The liquid level of the described water base nutrient solution of the bulb of described tissue culture seedlings of bananas bottom contact when initial, after new root grew, described liquid level was down to 4/5 place of root.The prescription of described water base nutrient solution is: contain in 1L water: potassium nitrate 300mg, ammonium nitrate 150mg, potassium dihydrogen phosphate 50mg, magnesium sulfate 250mg, calcium chloride 150mg, EDETATE SODIUM iron 40mg, boric acid 1.00mg, four hydrated manganese sulfate 2.50mg, Zinc vitriol 0.500mg, Salzburg vitriol 0.150mg and four molybdic acid hydrate 0.100mg.Described water base nutrient solution was changed once every 15 days.Described water base nutrient solution circulated once every 1 day.Every foliage fertilizer of spray in 7 days, described foliage fertilizer is the aqueous solution of 0.1 % by weight urea and 0.05 % by weight potassium dihydrogen phosphate.After cultivating through overnutrition, every young plant grows 5～7 young leaves, and natural height 15 is to 20cm.
Embodiment 2 water planting tissue culture seedlings of bananas of the present invention
Adopting PVC plate and tank is cultivating container.Use clear water to wash tissue culture seedlings of bananas (Brazilian any of several broadleaf plants off, available from Chinese Academy of Tropical Agricultural Sciences seedling group training center), the tissue culture seedlings of bananas of wash clean is inserted on the PVC plate, and the seedling spacing is 1cm, and the false basal part of stem of described tissue culture seedlings of bananas is higher than the liquid level of described water base culture of rootage liquid.Add water base culture of rootage liquid in the tank, under 35 ℃, carried out culture of rootage 10 days.The prescription of described water base culture of rootage liquid is: contain in 1L water: potassium nitrate 520mg, ammonium nitrate 100mg, potassium dihydrogen phosphate 150mg, magnesium sulfate 230mg, calcium chloride 350mg, EDETATE SODIUM iron 40mg, boric acid 1.00mg, four hydrated manganese sulfate 2.50mg, Zinc vitriol 0.900mg, Salzburg vitriol 0.100mg, four molybdic acid hydrate 0.100mg, NAA 0.5mg and 6-BA 0.1mg.
Afterwards, use water base nutrient solution to replace described water base culture of rootage liquid, under 20 ℃, carry out nutrition and cultivated 20 days.The liquid level of the described water base nutrient solution of the bulb of described tissue culture seedlings of bananas bottom contact when initial, after new root grew, described liquid level was down to 4/5 place of root.The prescription of described water base nutrient solution is: contain in 1L water: potassium nitrate 530mg, ammonium nitrate 50mg, potassium dihydrogen phosphate 150mg, magnesium sulfate 100mg, calcium chloride 350mg, EDETATE SODIUM iron 10mg, boric acid 1.50mg, four hydrated manganese sulfate 1.00mg, Zinc vitriol 0.900mg, Salzburg vitriol 0.100mg and four molybdic acid hydrate 0.150mg.Described water base nutrient solution was changed once every 10 days.Described water base nutrient solution circulated once every 2 days.Every foliage fertilizer of spray in 5 days, described foliage fertilizer is the aqueous solution of 0.1 % by weight urea and 0.05 % by weight potassium dihydrogen phosphate.After cultivating through overnutrition, every young plant grows 5～7 young leaves, and natural height 15 is to 20cm.
Embodiment 3 water planting tissue culture seedlings of bananas of the present invention
Adopting PVC plate and tank is cultivating container.Use clear water to wash tissue culture seedlings of bananas (Brazilian any of several broadleaf plants off, available from Chinese Academy of Tropical Agricultural Sciences seedling group training center), the tissue culture seedlings of bananas of wash clean is inserted on the PVC plate, and the seedling spacing is 1cm, and the false basal part of stem of described tissue culture seedlings of bananas is higher than the liquid level of described water base culture of rootage liquid.Add water base culture of rootage liquid in the tank, under 30 ℃, carried out culture of rootage 12 days.The prescription of described water base culture of rootage liquid is: contain in 1L water: potassium nitrate 505mg, ammonium nitrate 120mg, potassium dihydrogen phosphate 136mg, magnesium sulfate 246mg, calcium chloride 333mg, EDETATE SODIUM iron 24mg, boric acid 1.24mg, four hydrated manganese sulfate 2.23mg, Zinc vitriol 0.864mg, Salzburg vitriol 0.125mg, four molybdic acid hydrate 0.117mg, NAA 0.3mg and 6-BA 0.2mg.
Afterwards, use water base nutrient solution to replace described water base culture of rootage liquid, under 30 ℃, carry out nutrition and cultivated 25 days.The liquid level of the described water base nutrient solution of the bulb of described tissue culture seedlings of bananas bottom contact when initial, after new root grew, described liquid level was down to 4/5 place of root.The prescription of described water base nutrient solution is: contain in 1L water: potassium nitrate 505mg, ammonium nitrate 120mg, potassium dihydrogen phosphate 136mg, magnesium sulfate 246mg, calcium chloride 333mg, EDETATE SODIUM iron 24mg, boric acid 1.24mg, four hydrated manganese sulfate 2.23mg, Zinc vitriol 0.864mg, Salzburg vitriol 0.125mg and four molybdic acid hydrate 0.117mg.Described water base nutrient solution was changed once every 20 days.Described water base nutrient solution circulated once every 1 day.Every foliage fertilizer of spray in 6 days, described foliage fertilizer is the aqueous solution of 0.1 % by weight urea and 0.05 % by weight potassium dihydrogen phosphate.After cultivating through overnutrition, every young plant grows 5～7 young leaves, and natural height 15 is to 20cm.
Embodiment 4 booths dress cup is grown seedlings and Production of Large Fields
The seedling strain that obtains among the embodiment 1 to 3 is grown seedlings through booth dress cup, then carry out field planting, successfully plantation obtains banana, and the conventional husky bed of output is heeled in, and to cultivate the output that obtains banana higher.
Claims (8)
1. the method for a water planting tissue culture seedlings of bananas, the method comprises:
Step 1): make tissue culture seedlings of bananas in water base culture of rootage liquid, under 22 ℃ to 35 ℃, carry out culture of rootage 10 to 15 days; And
Step 2): use water base nutrient solution to replace described water base culture of rootage liquid, under 20 ℃ to 35 ℃, carry out nutrition and cultivated 20 to 30 days,
The prescription of described water base culture of rootage liquid is: contain in 1L water: potassium nitrate 500-520mg, ammonium nitrate 100-150mg, potassium dihydrogen phosphate 110-150mg, magnesium sulfate 230-250mg, calcium chloride 330-350mg, EDETATE SODIUM iron 20-40mg, boric acid 1.00-1.50mg, four hydrated manganese sulfate 2.00-2.50mg, Zinc vitriol 0.800-0.900mg, Salzburg vitriol 0.100-0.150mg, four molybdic acid hydrate 0.100-0.150mg, NAA 0.1-0.5mg and 6-BA 0.1-0.5mg
The prescription of described water base nutrient solution is: contain in 1L water: potassium nitrate 300-530mg, ammonium nitrate 50-150mg, potassium dihydrogen phosphate 50-150mg, magnesium sulfate 100-250mg, calcium chloride 150-350mg, EDETATE SODIUM iron 10-40mg, boric acid 1.00-1.50mg, four hydrated manganese sulfate 1.00-2.50mg, Zinc vitriol 0.500-0.900mg, Salzburg vitriol 0.100-0.150mg and four molybdic acid hydrate 0.100-0.150mg.
2. the method for water planting tissue culture seedlings of bananas according to claim 1, it is characterized in that, in described step 1), the prescription of described water base culture of rootage liquid is: contain in 1L water: potassium nitrate 505mg, ammonium nitrate 120mg, potassium dihydrogen phosphate 136mg, magnesium sulfate 246mg, calcium chloride 333mg, EDETATE SODIUM iron 24mg, boric acid 1.24mg, four hydrated manganese sulfate 2.23mg, Zinc vitriol 0.864mg, Salzburg vitriol 0.125mg, four molybdic acid hydrate 0.117mg, NAA 0.3mg and 6-BA 0.2mg.
3. the method for water planting tissue culture seedlings of bananas according to claim 1 and 2, it is characterized in that, in described step 1), the spacing of described tissue culture seedlings of bananas is not less than 0.5cm, and the false basal part of stem of described tissue culture seedlings of bananas is higher than the liquid level of described water base culture of rootage liquid.
4. the method for water planting tissue culture seedlings of bananas according to claim 1, it is characterized in that, in described step 2) in, the prescription of described water base nutrient solution is: contain in 1L water: potassium nitrate 505mg, ammonium nitrate 120mg, potassium dihydrogen phosphate 136mg, magnesium sulfate 246mg, calcium chloride 333mg, EDETATE SODIUM iron 24mg, boric acid 1.24mg, four hydrated manganese sulfate 2.23mg, Zinc vitriol 0.864mg, Salzburg vitriol 0.125mg and four molybdic acid hydrate 0.117mg.
5. the method for water planting tissue culture seedlings of bananas according to claim 4 is characterized in that, described water base nutrient solution was changed once every 10-20 days.
6. the method for water planting tissue culture seedlings of bananas according to claim 4 is characterized in that, described water base nutrient solution circulated once every 1-2 days.
7. the method for water planting tissue culture seedlings of bananas according to claim 4 is characterized in that, the liquid level of the described water base nutrient solution of the bulb of described tissue culture seedlings of bananas bottom contact when initial, and after new root grew, described liquid level was down to 4/5 place of root.
8. the method for water planting tissue culture seedlings of bananas according to claim 4 is characterized in that, every foliage fertilizer of spray in 5-7 days, described foliage fertilizer is the aqueous solution of 0.1 % by weight urea and 0.05 % by weight potassium dihydrogen phosphate.
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| CN102771326B (en) * | 2012-08-08 | 2013-04-17 | 东莞市香蕉蔬菜研究所 | Method for rapidly identifying and screening anti-blight banana seedling |
| CN102845287B (en) * | 2012-09-04 | 2014-06-25 | 中国热带农业科学院热带生物技术研究所 | Water-culture method and system of banana seedlings |
| CN103497989A (en) * | 2013-09-25 | 2014-01-08 | 中国热带农业科学院环境与植物保护研究所 | Method for detecting pathogenicity of pathogenic bacteria of banana vascular wilt |
| CN109122316A (en) * | 2018-08-28 | 2019-01-04 | 佛山科学技术学院 | A method of it improving the anti-silver ion of tissue culture seedlings of bananas and coerces ability |
| CN109287230A (en) * | 2018-11-14 | 2019-02-01 | 上海摩天农业科技有限公司 | Nutrient solution, nutrient solution raw materials composition and its application |
| CN110122213A (en) * | 2019-06-14 | 2019-08-16 | 福建农林大学 | A kind of method of the big bud implanting and cultivating of banana |
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