CN101768616B - 含稳定聚合酶的反应化合物的干组合物 - Google Patents

含稳定聚合酶的反应化合物的干组合物 Download PDF

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Publication number
CN101768616B
CN101768616B CN200910263837.4A CN200910263837A CN101768616B CN 101768616 B CN101768616 B CN 101768616B CN 200910263837 A CN200910263837 A CN 200910263837A CN 101768616 B CN101768616 B CN 101768616B
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liquid mixture
reaction
dry composition
compound
polymerase
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CN101768616A (zh
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T·哈斯林格
T·梅茨勒
A·佩塞尼
H·索贝克
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CN200910263837.4A 2008-12-19 2009-12-17 含稳定聚合酶的反应化合物的干组合物 Active CN101768616B (zh)

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EP08022082 2008-12-19
EP08022082.5 2008-12-19

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CN101768616A CN101768616A (zh) 2010-07-07
CN101768616B true CN101768616B (zh) 2014-07-09

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US (3) US8652811B2 (https=)
EP (1) EP2202302B1 (https=)
JP (2) JP5731113B2 (https=)
CN (1) CN101768616B (https=)
CA (1) CA2688174C (https=)
ES (1) ES2394754T3 (https=)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103003449A (zh) * 2010-05-06 2013-03-27 艾比斯生物科学公司 集成样品制备系统和稳定的酶混合物
US9845489B2 (en) 2010-07-26 2017-12-19 Biomatrica, Inc. Compositions for stabilizing DNA, RNA and proteins in saliva and other biological samples during shipping and storage at ambient temperatures
CA2806670A1 (en) 2010-07-26 2012-02-09 Biomatrica, Inc. Compositions for stabilizing dna, rna and proteins in blood and other biological samples during shipping and storage at ambient temperatures
WO2012045668A1 (en) 2010-10-04 2012-04-12 Roche Diagnostics Gmbh Method for cell lysis in a rt-pcr reaction buffer
WO2012045669A1 (en) 2010-10-04 2012-04-12 Roche Diagnostics Gmbh Method for cell lysis and pcr within the same reaction vessel
JP2013005796A (ja) * 2011-05-26 2013-01-10 Arkray Inc 乾燥試薬、乾燥試薬キット、試薬器、乾燥試薬の製造方法
EP2574931B1 (en) 2011-09-29 2017-03-22 Qiagen GmbH Dry composition comprising a control dye
EP3663408B1 (en) * 2011-10-11 2024-05-01 QIAGEN GmbH Sample processing method and sample processing cartridge
EP2776577B1 (en) 2011-11-07 2017-01-11 Qiagen GmbH Lysis method and lysis composition
EP2730653A1 (en) 2012-11-07 2014-05-14 QIAGEN GmbH Method for lysing a fixed biological sample
CN103849548A (zh) 2012-12-03 2014-06-11 三星电子株式会社 用于扩增核酸的试剂容器及其制备方法、存储试剂的方法和用于核酸分析的微流体系统
US9725703B2 (en) 2012-12-20 2017-08-08 Biomatrica, Inc. Formulations and methods for stabilizing PCR reagents
JP5904153B2 (ja) * 2013-03-29 2016-04-13 ソニー株式会社 核酸増幅反応用試料の調製方法、核酸増幅方法、固相状核酸増幅反応用試薬及びマイクロチップ
CN105431527A (zh) 2013-06-11 2016-03-23 比奥卡尔齐斯股份有限公司 用于长期储存的生物分子干燥方法
US10036061B2 (en) * 2013-12-20 2018-07-31 Roche Molecular Systems, Inc. Oligonucleotide inhibitor of DNA polymerases
CN106572650B (zh) 2014-06-10 2021-08-31 生物马特里卡公司 在环境温度下稳定凝血细胞
EP3015553A1 (de) * 2014-10-30 2016-05-04 Biotecon Diagnostics GmbH Stabilisierte Reaktionsmischung
SG11201804776SA (en) 2015-12-08 2018-07-30 Biomatrica Inc Reduction of erythrocyte sedimentation rate
GB201716963D0 (en) * 2017-10-16 2017-11-29 Biofortuna Ltd Compositions and methods for use in stabilising nucleic acid amplification assays
CN109486824B (zh) * 2018-11-20 2021-11-16 安徽省昂普拓迈生物科技有限责任公司 一种特异性结合Taq酶的核酸适配体及其筛选方法、应用
CN111961709A (zh) * 2020-01-21 2020-11-20 上海酷乐生物科技有限公司 一种用于口腔拭子直接荧光pcr扩增的试剂和试剂盒
EP4621069A1 (en) * 2022-11-18 2025-09-24 NOF Corporation Dry composition for nucleic acid amplification uses, and nucleic acid amplification method using same
CN115851889B (zh) * 2022-11-30 2025-12-05 北京全式金生物技术股份有限公司 松三糖和可逆结合因子Oligo在提升荧光定量PCR全预混体系的保存稳定性中的应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006131391A1 (en) * 2005-06-10 2006-12-14 Epigenomics Ag Prognostic assay for prediction of treatment response and/or survival of breast cell proliferative disorder patients

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3300474A (en) 1964-02-12 1967-01-24 Pharmacia Ab Sucrose ether copolymerizates
US5118801A (en) 1988-09-30 1992-06-02 The Public Health Research Institute Nucleic acid process containing improved molecular switch
GB8826429D0 (en) 1988-11-11 1988-12-14 Univ Leeds Ind Service Ltd Enzyme stabilisation systems
GB8903593D0 (en) 1989-02-16 1989-04-05 Pafra Ltd Storage of materials
US5270163A (en) 1990-06-11 1993-12-14 University Research Corporation Methods for identifying nucleic acid ligands
EP0786469B1 (en) 1990-06-11 2006-03-01 Gilead Sciences, Inc. Methods of use of nucleic acid ligands
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5200399A (en) 1990-09-14 1993-04-06 Boyce Thompson Institute For Plant Research, Inc. Method of protecting biological materials from destructive reactions in the dry state
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5565318A (en) 1994-09-02 1996-10-15 Pharmacia Biotech, Inc. Room temperature stable reagent semi-spheres
US5593824A (en) 1994-09-02 1997-01-14 Pharmacia Biotech, Inc. Biological reagent spheres
EP1033411B1 (en) 1996-06-04 2006-02-22 University of Utah Research Foundation Fluorescent donor-acceptor pair
JP4281877B2 (ja) 1996-06-04 2009-06-17 ユニバーシティ オブ ユタ リサーチ ファウンデーション 生物学的プロセスを実行し且つモニタリングするためのシステムと方法
EP1002875A1 (en) * 1998-11-17 2000-05-24 Mira Diagnostica GmbH An oligonucleotide inhibitor of thermostable polymerase
PT1232502E (pt) 1999-11-17 2006-05-31 Roche Diagnostics Gmbh Particulas de vidro magneticas, metodo para a sua preparacao e suas utilizacoes
WO2003057910A2 (en) 2002-01-08 2003-07-17 Roche Diagnostics Gmbh Use of silica material in an amplification reaction
US7407747B2 (en) * 2002-10-15 2008-08-05 Applera Corporation Method for drying dye-terminator sequencing reagents
WO2004090153A2 (en) * 2003-04-01 2004-10-21 Eragen Biosciences,Inc. Polymerase inhibitor and method of using same
EP1502958A1 (en) * 2003-08-01 2005-02-02 Roche Diagnostics GmbH New detection format for hot start real time polymerase chain reaction
US20070117094A1 (en) * 2003-09-30 2007-05-24 Yoshihide Hayashizaki Support, printed material and reagent kit having enzyme fixed thereon, method for preparing the support, method of storing enzyme and method for restoration enzymes
US20060099567A1 (en) * 2004-04-08 2006-05-11 Biomatrica, Inc. Integration of sample storage and sample management for life science
JP5254949B2 (ja) * 2006-03-15 2013-08-07 マイクロニクス， インコーポレイテッド 一体型の核酸アッセイ
CA2653454A1 (en) * 2006-05-23 2007-11-29 Molecular Detection, Inc. Ambient temperature stable kits for molecular diagnostics
EP2063866B2 (en) 2006-09-18 2017-08-23 GE Healthcare Bio-Sciences Corp. Preparation of glassified biological reagents
DE102006056790B3 (de) * 2006-12-01 2008-01-17 IfP Privates Institut für Produktqualität GmbH Laboreinmalartikel für Analyse und Diagnosik
KR20100049019A (ko) * 2007-06-16 2010-05-11 이니그마 다이아그노스틱스 리미티드 조성물

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006131391A1 (en) * 2005-06-10 2006-12-14 Epigenomics Ag Prognostic assay for prediction of treatment response and/or survival of breast cell proliferative disorder patients

Also Published As

Publication number Publication date
JP5731113B2 (ja) 2015-06-10
EP2202302A1 (en) 2010-06-30
US20160237473A1 (en) 2016-08-18
US8652811B2 (en) 2014-02-18
US20100159529A1 (en) 2010-06-24
JP2010142229A (ja) 2010-07-01
US20140162267A1 (en) 2014-06-12
ES2394754T3 (es) 2013-02-05
CN101768616A (zh) 2010-07-07
US9631233B2 (en) 2017-04-25
CA2688174A1 (en) 2010-06-19
JP2015091261A (ja) 2015-05-14
EP2202302B1 (en) 2012-09-12
US9163286B2 (en) 2015-10-20
CA2688174C (en) 2018-08-07

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