JP5731113B2 - 反応化合物と安定化ポリメラーゼの乾燥組成物 - Google Patents

反応化合物と安定化ポリメラーゼの乾燥組成物 Download PDF

Info

Publication number
JP5731113B2
JP5731113B2 JP2009286615A JP2009286615A JP5731113B2 JP 5731113 B2 JP5731113 B2 JP 5731113B2 JP 2009286615 A JP2009286615 A JP 2009286615A JP 2009286615 A JP2009286615 A JP 2009286615A JP 5731113 B2 JP5731113 B2 JP 5731113B2
Authority
JP
Japan
Prior art keywords
dry composition
aptamer
liquid mixture
polymerase
reaction
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2009286615A
Other languages
English (en)
Japanese (ja)
Other versions
JP2010142229A5 (https=
JP2010142229A (ja
Inventor
ハスリンガー トビアス
ハスリンガー トビアス
メッツラー トーマス
メッツラー トーマス
ペツェニー アンネット
ペツェニー アンネット
ゾベク ハーラルト
ゾベク ハーラルト
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=40510578&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=JP5731113(B2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of JP2010142229A publication Critical patent/JP2010142229A/ja
Publication of JP2010142229A5 publication Critical patent/JP2010142229A5/ja
Application granted granted Critical
Publication of JP5731113B2 publication Critical patent/JP5731113B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2009286615A 2008-12-19 2009-12-17 反応化合物と安定化ポリメラーゼの乾燥組成物 Active JP5731113B2 (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP08022082.5 2008-12-19
EP08022082 2008-12-19

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2015003548A Division JP2015091261A (ja) 2008-12-19 2015-01-09 反応化合物と安定化ポリメラーゼの乾燥組成物

Publications (3)

Publication Number Publication Date
JP2010142229A JP2010142229A (ja) 2010-07-01
JP2010142229A5 JP2010142229A5 (https=) 2012-01-26
JP5731113B2 true JP5731113B2 (ja) 2015-06-10

Family

ID=40510578

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2009286615A Active JP5731113B2 (ja) 2008-12-19 2009-12-17 反応化合物と安定化ポリメラーゼの乾燥組成物
JP2015003548A Pending JP2015091261A (ja) 2008-12-19 2015-01-09 反応化合物と安定化ポリメラーゼの乾燥組成物

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2015003548A Pending JP2015091261A (ja) 2008-12-19 2015-01-09 反応化合物と安定化ポリメラーゼの乾燥組成物

Country Status (6)

Country Link
US (3) US8652811B2 (https=)
EP (1) EP2202302B1 (https=)
JP (2) JP5731113B2 (https=)
CN (1) CN101768616B (https=)
CA (1) CA2688174C (https=)
ES (1) ES2394754T3 (https=)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG10201503540QA (en) * 2010-05-06 2015-06-29 Ibis Biosciences Inc Integrated sample preparation systems and stabilized enzyme mixtures
EP2598660B1 (en) 2010-07-26 2017-03-15 Biomatrica, INC. Compositions for stabilizing dna, rna and proteins in blood and other biological samples during shipping and storage at ambient temperatures
WO2012018639A2 (en) 2010-07-26 2012-02-09 Biomatrica, Inc. Compositions for stabilizing dna, rna and proteins in saliva and other biological samples during shipping and storage at ambient temperatures
CA2810316A1 (en) 2010-10-04 2012-04-12 F. Hoffmann-La Roche Ag Method for cell lysis and pcr within the same reaction vessel
JP5798631B2 (ja) 2010-10-04 2015-10-21 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ Ｒｔ−ｐｃｒ反応緩衝液中での細胞溶解のための方法
JP2013005796A (ja) * 2011-05-26 2013-01-10 Arkray Inc 乾燥試薬、乾燥試薬キット、試薬器、乾燥試薬の製造方法
EP2574931B1 (en) 2011-09-29 2017-03-22 Qiagen GmbH Dry composition comprising a control dye
WO2013053855A1 (en) * 2011-10-11 2013-04-18 Qiagen Gmbh Sample processing method and sample processing cartridge
WO2013068107A1 (en) 2011-11-07 2013-05-16 Qiagen Gmbh Lysis method and lysis composition
EP2730653A1 (en) 2012-11-07 2014-05-14 QIAGEN GmbH Method for lysing a fixed biological sample
CN103849548A (zh) 2012-12-03 2014-06-11 三星电子株式会社 用于扩增核酸的试剂容器及其制备方法、存储试剂的方法和用于核酸分析的微流体系统
US9725703B2 (en) 2012-12-20 2017-08-08 Biomatrica, Inc. Formulations and methods for stabilizing PCR reagents
JP5904153B2 (ja) * 2013-03-29 2016-04-13 ソニー株式会社 核酸増幅反応用試料の調製方法、核酸増幅方法、固相状核酸増幅反応用試薬及びマイクロチップ
CN105431527A (zh) 2013-06-11 2016-03-23 比奥卡尔齐斯股份有限公司 用于长期储存的生物分子干燥方法
US10036061B2 (en) 2013-12-20 2018-07-31 Roche Molecular Systems, Inc. Oligonucleotide inhibitor of DNA polymerases
ES2786373T3 (es) 2014-06-10 2020-10-09 Biomatrica Inc Estabilización de trombocitos a temperaturas ambiente
EP3015553A1 (de) * 2014-10-30 2016-05-04 Biotecon Diagnostics GmbH Stabilisierte Reaktionsmischung
KR20250047404A (ko) 2015-12-08 2025-04-03 바이오매트리카 인코포레이티드 적혈구 침강 속도의 감소
GB201716963D0 (en) * 2017-10-16 2017-11-29 Biofortuna Ltd Compositions and methods for use in stabilising nucleic acid amplification assays
CN109486824B (zh) * 2018-11-20 2021-11-16 安徽省昂普拓迈生物科技有限责任公司 一种特异性结合Taq酶的核酸适配体及其筛选方法、应用
CN111961709A (zh) * 2020-01-21 2020-11-20 上海酷乐生物科技有限公司 一种用于口腔拭子直接荧光pcr扩增的试剂和试剂盒
WO2024106507A1 (ja) * 2022-11-18 2024-05-23 日油株式会社 核酸増幅用の乾燥組成物およびそれを用いた核酸増幅法
CN115851889B (zh) * 2022-11-30 2025-12-05 北京全式金生物技术股份有限公司 松三糖和可逆结合因子Oligo在提升荧光定量PCR全预混体系的保存稳定性中的应用

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3300474A (en) 1964-02-12 1967-01-24 Pharmacia Ab Sucrose ether copolymerizates
US5118801A (en) 1988-09-30 1992-06-02 The Public Health Research Institute Nucleic acid process containing improved molecular switch
GB8826429D0 (en) 1988-11-11 1988-12-14 Univ Leeds Ind Service Ltd Enzyme stabilisation systems
GB8903593D0 (en) 1989-02-16 1989-04-05 Pafra Ltd Storage of materials
ES2259800T3 (es) 1990-06-11 2006-10-16 Gilead Sciences, Inc. Procedimientos de uso de ligandos de acido nucleico.
US5270163A (en) 1990-06-11 1993-12-14 University Research Corporation Methods for identifying nucleic acid ligands
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5200399A (en) 1990-09-14 1993-04-06 Boyce Thompson Institute For Plant Research, Inc. Method of protecting biological materials from destructive reactions in the dry state
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5593824A (en) 1994-09-02 1997-01-14 Pharmacia Biotech, Inc. Biological reagent spheres
US5565318A (en) 1994-09-02 1996-10-15 Pharmacia Biotech, Inc. Room temperature stable reagent semi-spheres
JP4540754B2 (ja) 1996-06-04 2010-09-08 ユニバーシティ オブ ユタ リサーチ ファウンデーション Ｐｃｒ中のハイブリダイゼーションのモニタリング
ES2253778T3 (es) 1996-06-04 2006-06-01 University Of Utah Research Foundation Sistema y metodos de monitorizacion de una amplificacion de adn mediante fluorescencia.
EP1002875A1 (en) * 1998-11-17 2000-05-24 Mira Diagnostica GmbH An oligonucleotide inhibitor of thermostable polymerase
WO2001037291A1 (en) 1999-11-17 2001-05-25 Roche Diagnostics Gmbh Magnetic glass particles, method for their preparation and uses thereof
WO2003057910A2 (en) 2002-01-08 2003-07-17 Roche Diagnostics Gmbh Use of silica material in an amplification reaction
US7407747B2 (en) * 2002-10-15 2008-08-05 Applera Corporation Method for drying dye-terminator sequencing reagents
AU2004227353B2 (en) * 2003-04-01 2010-02-25 Luminex Corporation Polymerase inhibitor and method of using same
EP1502958A1 (en) * 2003-08-01 2005-02-02 Roche Diagnostics GmbH New detection format for hot start real time polymerase chain reaction
US20070117094A1 (en) * 2003-09-30 2007-05-24 Yoshihide Hayashizaki Support, printed material and reagent kit having enzyme fixed thereon, method for preparing the support, method of storing enzyme and method for restoration enzymes
US20060099567A1 (en) * 2004-04-08 2006-05-11 Biomatrica, Inc. Integration of sample storage and sample management for life science
WO2006131391A1 (en) * 2005-06-10 2006-12-14 Epigenomics Ag Prognostic assay for prediction of treatment response and/or survival of breast cell proliferative disorder patients
ES2393758T3 (es) * 2006-03-15 2012-12-27 Micronics, Inc. Ensayos integrados de ácidos nucleicos
EP2210955A3 (en) * 2006-05-23 2010-09-15 Molecular Detection, Inc. Ambient temperature stable kits for molecular diagnostics
JP5989957B2 (ja) 2006-09-18 2016-09-07 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション ガラス化生物試薬の調製
DE102006056790B3 (de) * 2006-12-01 2008-01-17 IfP Privates Institut für Produktqualität GmbH Laboreinmalartikel für Analyse und Diagnosik
KR20100049019A (ko) * 2007-06-16 2010-05-11 이니그마 다이아그노스틱스 리미티드 조성물

Also Published As

Publication number Publication date
CA2688174C (en) 2018-08-07
US20160237473A1 (en) 2016-08-18
ES2394754T3 (es) 2013-02-05
US20140162267A1 (en) 2014-06-12
US9631233B2 (en) 2017-04-25
US8652811B2 (en) 2014-02-18
JP2010142229A (ja) 2010-07-01
US9163286B2 (en) 2015-10-20
CA2688174A1 (en) 2010-06-19
EP2202302B1 (en) 2012-09-12
US20100159529A1 (en) 2010-06-24
EP2202302A1 (en) 2010-06-30
JP2015091261A (ja) 2015-05-14
CN101768616A (zh) 2010-07-07
CN101768616B (zh) 2014-07-09

Similar Documents

Publication Publication Date Title
JP5731113B2 (ja) 反応化合物と安定化ポリメラーゼの乾燥組成物
KR101829182B1 (ko) 표적 핵산의 바코딩을 위한 멀티 프라이머 증폭 방법
ES3022638T3 (en) Recombinase polymerase amplification reagents
EP2021508B1 (en) Ambient temperature stable kits for molecular diagnostics
JP2008500831A5 (https=)
JP6898238B2 (ja) 安定した反応混合物
US9273303B2 (en) Dried and stabilized ready-to-use composition containing nucleic acid polymerization enzymes for molecular biology applications
WO2017184028A1 (en) Stabilized mixture of reagents for molecular diagnostics
JP5608998B2 (ja) 保存安定性に優れた核酸増幅検出試薬キット
JP2007520236A (ja) 抗凍結タンパク質により高められた核酸増幅
US20210269850A1 (en) Dry compositions for use in nucleic acid amplification and methods
JP4855413B2 (ja) 核酸を増幅するための組成物
US10227642B2 (en) Detergent free polymerases
JP5608997B2 (ja) 保存安定性に優れた核酸増幅検出試薬キット
US20060110745A1 (en) Method for amplifying nucleic acids

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20111206

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20111206

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20130903

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20131202

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20131205

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20140303

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20140909

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20150109

A911 Transfer to examiner for re-examination before appeal (zenchi)

Free format text: JAPANESE INTERMEDIATE CODE: A911

Effective date: 20150116

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20150310

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20150409

R150 Certificate of patent or registration of utility model

Ref document number: 5731113

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250