CN101755799B - Cyazofamid-containing bactericidal composition - Google Patents
Cyazofamid-containing bactericidal composition Download PDFInfo
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Abstract
The invention relates to a synergistic bactericidal composition, in particular to the synergistic bactericidal composition containing cyazofamid and any one of bactericidal active ingredients chosen from thiophanate methyl, dimethomorph, cymoxanil, metalaxyl and oxadixyl.
Description
Technical field
The present invention relates to a kind of Synergistic bactericidal composition, particularly relate to a kind of Synergistic bactericidal composition that comprises the cyazofamid arbitrary fungicide active ingredient clever with being selected from thiophanate-methyl, dimethomorph, white urea cyanogen, metalaxyl He Evil frost.
Background technology
Cyazofamid (cyazofamid), chemical name is 4-chloro-2-cyano group-N, N-dimethyl-5-p-methylphenyl imidazoles-1-sulfonamide, be the sulphaguanidine azoles fungicide, the Oomycete fungi had strong bactericidal activity, each vegetative stage to phytophthora history of life comprises sporangial formation, sporangial sprouting, and the movement of the formation of egg spore, the release of zoospore, zoospore and the growth of mycelia etc. all have very high inhibition.Compare with other medicament, cyazofamid has high prolection to potato late blight, cucumber downy mildew.
Thiophanate-methyl (thiophanate-methyl), chemical name are two (3-methoxycarbonyl group-α-ghiourea group) benzene of 1,2-, belong to benzimidazole, be a kind of broad spectrum activity systemic fungicide, can prevent and treat the various crop disease, have interior suction, preventive and therapeutic action.It is converted into carbendazim in plant corpus, the formation of spindle affects cell division in the mitosis of interference bacterium.
Dimethomorph (dimethomorph), chemical name is (E, Z)-4-[3-(4-chlorphenyl)-3-(4-chlorphenyl)-3-(3,4-Dimethoxyphenyl-acryloyl) morpholine belongs to the morpholine series bactericidal agent, is known systemic fungicide, its effect characteristics are to destroy the formation of cell envelope, stages to oomycetes history of life has effect, and is particularly responsive at the formation stages of sporangiophore and egg spore, and Peronospora and Phytophthora fungi are had preferably preventive effect.
Frost urea cyanogen (cymoxanil); chemical name is 2-cyano group-N-[(ethylamino-) carbonyl]-2-(methoxyl group imido grpup) acetamide; it is a kind of high-efficiency low-toxicity bactericide; belong to the substituted urea class bactericide; have contact and local systemic action, can suppress spore germination, use with to prolong the lasting period with protective fungicide; be widely used in the upper Peronosporales fungi such as the brassicaceous vegetable such as cucumber, grape, tomato, lichee, huge dish and tobacco, belong to such as epidemic disease frost genus, Peronospora, single shaft frost.
Metalaxyl (metalaxyl); chemical name is D; L-N-(2; the 6-3,5-dimethylphenyl)-N-(2 '-methoxyl group acetyl) methyl lactamine; belong to the substituted benzene acid amide fungicides; its effect characteristics are suppress its protein and nucleic acid synthetic; the systemic fungicide of tool protection and therapeutic action; can be absorbed by the root of plant, stem, leaf; and transfer to each organ of plant with moisture content in the plant corpus; can make cauline leaf and process, seed treatment and soil treatment are effective to downy mildew, phytophthora, the caused disease of pythium spp.
Evil frost spirit (oxadixyl), chemical name be 2-methoxyl group-N-(2-oxo-1,3-oxazoles alkane-3-yl) acetyl-2 ', 6 '-dimethylaniline, genus phenylamide systemic fungicide has contact sterilization and interior suction conduction.Thereby its mechanism of action suppresses the biosynthesis of RNA for suppressing RNA polymerase, the Peronosporales pathogen is had special efficacy.
The inventor studies have shown that by a series of, the cyazofamid arbitrary fungicide active ingredient clever with being selected from thiophanate-methyl, dimethomorph, white urea cyanogen, metalaxyl He Evil frost is mixed the target bacterium had extremely strong synergistic effect, greatly improve bactericidal activity, thereby finished the present invention.
Summary of the invention
The object of the present invention is to provide a kind of mixed ratio reasonable, control efficiency is good, the Synergistic bactericidal composition that drug cost is low.
Another object of the present invention is to provide by formulations such as the formulated missible oil of this bactericidal composition, microemulsion, aqueous emulsion, suspending agent, wetting powder, water dispersible granules, granules.
Another object of the present invention is to provide the purposes that bactericidal composition is used for downy mildew that control causes by the Oomycete disease fungus, epidemic disease, late blight.
Technical scheme of the present invention is as described below:
A kind of bactericidal composition is characterized in that containing the cyazofamid of synergy effective dose and is selected from thiophanate-methyl, dimethomorph, white urea cyanogen, metalaxyl He arbitrary fungicide active ingredient of Evil frost spirit.
Wherein, cyazofamid be selected from thiophanate-methyl, dimethomorph, white urea cyanogen, metalaxyl with the weight ratio of arbitrary fungicide active ingredient of Evil frost spirit can change in very wide scope, be preferably 40: 1～1: 40, more preferably 10: 1-1: 15.
And, the weight ratio of cyazofamid and thiophanate-methyl more preferably 1: 1～1: 12; The weight ratio of cyazofamid and dimethomorph more preferably 3: 1～1: 5; The weight ratio of cyazofamid and the spirit of white urea Qing Huo Evil frost more preferably 1: 3～3: 1; The weight ratio of cyazofamid and metalaxyl more preferably 5: 1～1: 5.
Active component is 0.5% to 95% of gross weight in the composition of the present invention.
Except the fungicidal activity composition, also can comprise functional aid in the bactericidal composition of the present invention.That is to say that the present composition can add other components such as functional aid, be mixed with the preparation of missible oil, aqueous emulsion, microemulsion, suspending agent, wetting powder, water dispersible granules or other formulations.
In these preparations, except active component, all contain surfactant, and also can contain the thinners such as organic solvent or cosolvent, carrier (filler) or water according to different dosage form, add in case of necessity other functional aids such as antifreeze, thickener, stabilizing agent, defoamer, disintegrant.Surfactant can be emulsifier, dispersant, wetting agent or bleeding agent.Surfactant can be nonionic with ionic, also can be two or more compound uses.
Described emulsifier comprises alkylphenol-polyethenoxy (polyoxypropylene) ether, Ben-zylphenol Polyoxyethyl Ether, phenethyl phenol polyethenoxy ether, fatty alcohol-polyoxyethylene ether, polyoxyethylene carboxylate, phenethyl phenol polyethenoxy polyethenoxy ether, castor oil ethylene oxide adduct, alkylphenol polyoxyethylene formaldehyde condensation products, and the phosphate of these compounds, alkyl benzene sulphonate (calcium salt or sodium salt) or directly use commercially available emulsifier monomer and composite product.Concrete example is such as farming breast 500
#Series, 600
#Series, 1600
#Series, NP is serial, OP is serial, AEO is serial, 700
#, 400
#Serial and composite kind 2201,0203B, 2021B, 4103 etc., these all are emulsifier productions well known in the art, market is on sale.
Described dispersant; wetting agent; bleeding agent comprises lignosulfonates (sodium salt or calcium salt); Negel; condensation compound of methyl naphthalene sulfonic acid and formaldehyde sulphate; naphthalene sulfonic acid-formaldehyde condensation product; methanonaphthalene sodium sulfonate dibutyl naphthalene sulfonic acid-formaldehyde condensation product; N-methyl fatty acyl group sodium taurocholate; alkylphenol polyoxyethylene formaldehyde condensation products sulphate; polymerization of carboxylic acid sodium; the alkylphenol polyoxyethylene phosphate; the withered powder of tea; saponin; lignosulfite; aliphatic alcohol sulfate; dodecyl sodium sulfate; alkylbenzenesulfonate; penetrant t; bleeding agent OT; fatty acid amide-N methyl taurine sodium salt; sodium sulfate of polyethenoxy ether of fatty alcohol; pull open powder; JFC; can use separately, but also compound use.Above-mentioned surfactant all is materials well known in the art, can obtain by various commercial channel.
Described solvent, cosolvent comprise toluene, dimethylbenzene, chlorobenzene, solvent naphtha, alpha-methyl-naphthalene, turpentine oil, carrene, chloroform, methyl alcohol, ethanol, isopropyl alcohol, butanols, isoamyl alcohol, cyclohexanone, isophorone, acetophenone, ethyl acetate, butyl acetate, N.N-dimethyl formamide, dimethyl sulfoxide (DMSO), methyl cellosolve, ethyl cellosolve etc.
Described antifreeze comprises ethylene glycol, glycerine, propane diols, urea, glycerine, ether diglycol, polyethylene glycol, isopropyl alcohol etc.
Described carrier, filler comprise white carbon, precipitated calcium carbonate, potter's clay, attapulgite, kaolin, diatomite, bentonite, sepiolite, zeolite, quartz sand, talcum powder, can use separately, also can two kinds or above mixing use.
Described disintegrant has sodium alginate, sodium carboxymethyl starch, sulfuric acid amine, bentonite, urea, calcium chloride.
Described defoamer has bubble enemy, silicone, C
8-10Fatty alcohol, C
10-20Saturated fat acids (such as capric acid, stearic acid etc.) and amide-type.
Described binding agent comprises starch, dextrin, gum Arabic, soybean protein, gelatine, sodium sulphate, gypsum, polyvinyl alcohol, polyethylene glycol, rosin, paraffin, CMC, sodium silicate.Can use separately, also can two or morely use with.
Can contain toner in the preparation, inorganic pigment iron oxide for example, titanium oxide or Prussian blue; Organic dyestuff is such as A Lizhalin, azo dyes, metal phthalocyanine or kiton colors.In water-base preparation, for preventing moldy metamorphism, need to add a certain amount of preservative, described preservative has Sodium Benzoate, polyformaldehyde, sorbitol, formalin, citric acid, methyl p-methyl benzoate, O-SyL sodium, 1,2-benzisothiazole-3-ketone (code name BIT) etc.
The product that the present invention describes can be the finished product preparation form to be provided, and namely each material mixes in the composition, but the composition of composition also can provide with independent preparation directly mixing in bucket (tank) before using.Concentrate of the present invention usually and water be mixed to get the concentration of required active substance.
Composition of the present invention can be used for various plants, such as Chinese cabbage, wild cabbage, cauliflower, wax gourd, cucumber, tomato, lichee, grape, wheat, rape, potato, tobacco and flowers etc.The place of using of the product that composition of the present invention and carrier are made is farmland, orchard or warehouse etc.
Composition of the present invention also can with other compound with weeding, desinsection or bactericidal property particularly the protective fungicide agent composition use, also can use with nematocide, protective agent, growth regulator, nutrient for plants or soil conditioner mixture.Composition of the present invention can be used by common method, such as cast, injection, spraying.Amount of application of the present invention is with weather condition or crop state variation, and time of application can be before or after crop be susceptible.The duration of protective effect is usually relevant with the content of individualized compound in the composition, also relevant with extraneous factor, weather for example, but by using suitable formulation can slow down climatic influences.
The remarkable advantage of composition of the present invention: the one, composition shows extremely strong synergistic effect in certain ratio range, the bactericidal effect of mixed composition is significantly improved than its single dose, thereby reduced using dosage, when reducing peasant's drug cost, reduced influence degree.The 2nd, interracial structure differs larger, and the resistance that the application of said composition can delay single dose occurs and development.
Embodiment:
The present invention is elaborated with following specific embodiment, so that advantage of the present invention is more obvious, but the present invention is limited to absolutely not these examples.Percentage all is weight percentage in the prescription of all preparations, and the processing of biologically-active moiety is effective content.
One, Preparation Example:
1, the preparation of missible oil
By the prescription requirement, add respectively solvent, former medicine, emulsifier, mix, use in case of necessity the hot bath heating for dissolving, namely obtain transparence missible oil.Such as embodiment 1～3 prescription.
Embodiment 1 (45% missible oil)
Cyazofamid 5%, thiophanate-methyl 40%, farming breast 500
#7.5%, agricultural newborn 600-2
#4.5%, methyl alcohol 8%, dimethylbenzene complements to 100%.
Embodiment 2 (15% missible oil)
Cyazofamid 5%, dimethomorph 10%, farming breast 507
#10%, agricultural newborn OX-653 3%, methyl alcohol 10%, dimethylbenzene complements to 100%.
Embodiment 3 (10% missible oil)
Cyazofamid 5%, white urea cyanogen 5%, farming breast 500
#10%, agricultural newborn OX-635 5%, methyl alcohol 10%, dimethylbenzene complements to 100%.
2, the preparation of wetting powder
By the prescription requirement, former medicine, various auxiliary agent and filler etc. are fully mixed, behind ultra-fine pulverizer disintegrating, namely obtain converted products.Such as embodiment 4～10 prescriptions.
Embodiment 4 (45% wetting powder)
Cyazofamid 5%, thiophanate-methyl 40%, lauryl sodium sulfate 1.5%, carboxymethyl cellulose 1%, sodium lignin sulfonate 10%, precipitated calcium carbonate complements to 100%.
Embodiment 5 (15% wetting powder)
Cyazofamid 5%, dimethomorph 10%, lauryl sodium sulfate 1.5%, carboxymethyl cellulose 1%, sodium lignin sulfonate 10%, precipitated calcium carbonate complements to 100%.
Embodiment 6 (15% wetting powder)
Cyazofamid 5%, metalaxyl 10%, neopelex 1%, white carbon 10%, sodium lignin sulfonate 10%, precipitated calcium carbonate complements to 100%.
Embodiment 7 (10% wetting powder)
Cyazofamid 5%, white urea cyanogen 5%, neopelex 1%, white carbon 10%, sodium lignin sulfonate 10%, precipitated calcium carbonate complements to 100%.
Embodiment 8 (10% wetting powder)
Cyazofamid 5% ， Evil frost spirit 5%, alkyl amide taurate 2%, Fluhyzon formaldehyde condensation 5%, alkylphenol-polyethenoxy phosphate 5%, precipitated calcium carbonate complements to 100%.
3, the preparation of aqueous emulsion
Former medicine, solvent, emulsifier and co-emulsifier are added together, make dissolving be even oil phase.Water, antifreeze etc. is mixed, becomes the homogeneous water.Under high-speed stirred, water is joined oil phase or oil phase is joined water, form the aqueous emulsion of favorable dispersibility.Such as embodiment 10～13 prescriptions.
Embodiment 11 (27% aqueous emulsion)
Cyazofamid 3%, thiophanate-methyl 24%, polyvinyl alcohol 0.8%, in triphenylethylene phenol+oxirane, phosphorylation, the triethanolamine and 8.5%, alkyl-diethylene glycol ether-sodium sulfonate 2.5%, farming breast 2,201 13%, dimethyl formamide 8%, ethylene glycol 5%, water complements to 100%.
Embodiment 12 (10% aqueous emulsion)
Cyazofamid 4%, dimethomorph 6%, polyvinyl alcohol 0.9%, alkylaryl polyoxyethylene propylene ether 8.5%, farming breast 2,201 15%, dimethyl formamide 12%, ethylene glycol 5%, water complements to 100%.
Embodiment 13 (6% aqueous emulsion)
Cyazofamid 3%, white urea cyanogen 3%, polyvinyl alcohol 1.5%, alkylaryl polyoxyethylene propylene ether 10%, farming breast 2,201 15%, dimethyl formamide 15%, ethylene glycol 5%, water complements to 100%.
4, the preparation of water-dispersible granules
Former medicine and powder carrier, wet spreading agent and binding agent etc. are carried out co-grinding, after adding again water and mediating, add in the comminutor that the certain specification screen cloth is housed and carry out granulation.And then drying, screening (by the screen cloth scope) namely gets granular product.Such as embodiment 14～15 prescriptions.
Embodiment 14 (15% water-dispersible granules)
Cyazofamid 6%, dimethomorph 9%, methyl naphthalene sulfonate formaldehyde condensate 12%, epoxidized polyether 5%, soluble starch 15%, gypsum complements to 100%.
Embodiment 15 (15% water-dispersible granules)
Cyazofamid 7.5%, white urea cyanogen 7.5%, methanonaphthalene sodium sulfonate 5%, epoxidized polyether 5%, sodium sulphate, gypsum complements to 100%.
5, the preparation of suspending agent
By the prescription requirement, take water as medium, former medicine, dispersant, suspending agent and antifreezing agent etc. are added in the sand milling still, carry out porphyrize, make suspending agent.Such as embodiment 16～18 prescriptions.
Embodiment 16 (45% suspending agent)
Cyazofamid 5%, thiophanate-methyl 40%, methyl naphthalene sulfonate formaldehyde condensate 5.5%, white carbon 0.2%, aluminium-magnesium silicate 0.18%, ethylene glycol 4%, silicone a little, water complements to 100%.
Embodiment 17 (15% suspending agent)
Cyazofamid 5%, dimethomorph 10%, calcium lignosulfonate 5%, white carbon 0.3%, ethylene glycol 4%, silicone a little, water complements to 100%.
Embodiment 18 (15% suspending agent)
Cyazofamid 5%, metalaxyl 10%, methyl naphthalene sulfonic acid naphthaldehyde condensation compound 2.5%, bentonite 1.0%, farming breast 1,601 3%, glycerine 4%, silicone a little, water complements to 100%.
6, the preparation of microemulsion
Former medicine, solvent, emulsifier and co-emulsifier are added together, make dissolving be even oil phase.Water, antifreeze etc. is mixed, becomes the homogeneous water.Under high-speed stirred, water is joined oil phase or oil phase is joined water, form the microemulsion of favorable dispersibility.Such as embodiment 19～21 prescriptions.
Embodiment 19 (27% microemulsion)
Cyazofamid 3%, thiophanate-methyl 24%, propyl alcohol 7%, phenethyl phenol polyethenoxy ether 10%, carrene 10%, glycerine 5%, water complements to 100%.
Embodiment 20 (10% microemulsion)
Cyazofamid 5%, white urea cyanogen 5%, dimethylbenzene 12%, phenyl xenol polyvinylether 15%, ring ethanol 8%, ethylene glycol 8%, water complements to 100%.
Embodiment 21 (10% microemulsion)
Cyazofamid 4%, metalaxyl 6%, ethanol 6%, sodium alkyl benzene sulfonate 8%, the third hexanone 8%, propane diols 6%, water complements to 100%.
7, the present invention illustrates the synergistic effect that the present invention makes up with following biologically active embodiment.
The joint toxicity measuring of test example 1 cyazofamid and thiophanate-methyl
For the examination bacterium
Cucumber downy mildew (Psenudoperonos pora.Cubensis)
For the examination cucumber variety
" the close thorn in Shandong ", cucumber is potted plant to be cultured to two open and flat phases of few leaf in the greenhouse.
The experimental concentration design
The experimental concentration of combination medicament and cyazofamid single dose is five concentration of 40,20,10,5,2.5 μ g/ml, and the thiophanate-methyl concentration for the treatment of is 80,40,20,10,5 μ g/ml, and other establishes the blank of not dosing, and each is processed 4 times and repeats.
Test method
Select second true leaf of the potted plant cucumber of growth neat and consistent, put to culture dish at petiole place humidification cotton balls after cutting, by top set concentration in the crops sprayer processing of spraying, after the blade liquid dries, in second day inoculation cucumber downy mildew germ, then place in the crop cultivation case and cultivate.
Condition of culture
Cultivation temperature: 28 ℃ of daytimes, 23 ℃ of nights.Incubation time: 6 days.
Investigation and method of counting
6 days " Invest, Then Investigate " control efficiency are cultivated in moisturizing in incubator, by the grade scale classification record of medicine inspecting institute of the Ministry of Agriculture " agricultural chemicals field trial criterion ", calculate control efficiency with disease index, and obtain virulence regression equation and EC
50, according to Wadley fungicide in mixture synergy evaluation assessment, calculate interactional ratio behind two single dose mixtures (be that SR＜0.5 is antagonism, 0.5≤SR≤1.5 are summation action, and SR＞1.5 are synergistic effect).
Result of the test sees the following form 1.
The joint toxicity measuring result of table 1 cyazofamid and thiophanate-methyl
Result of the test shows: cyazofamid and thiophanate-methyl weight ratio are 1: 8 o'clock, and its interaction ratio is respectively 1.81, and the bactericide interaction theory according to GiSi (1985) proposes has the notable synergistic effect.
The joint toxicity measuring of test example 2 cyazofamids and dimethomorph
For the examination bacterium
Cucumber downy mildew (Psenudoperonos pora.Cubensis)
For the examination cucumber variety
" the close thorn in Shandong ", cucumber is potted plant to be cultured to two open and flat phases of few leaf in the greenhouse.
The experimental concentration design
The combination medicament experimental concentration is five concentration of 32,16,8,4,2 μ g/ml, and cyazofamid and dimethomorph concentration for the treatment of are 40,20,10,5,2.5 μ g/ml, and other establishes the blank of not dosing, and each is processed 4 times and repeats.
Test method
Select second true leaf of the potted plant cucumber of growth neat and consistent, put to culture dish at petiole place humidification cotton balls after cutting, by top set concentration in the crops sprayer processing of spraying, after the blade liquid dries, in second day inoculation cucumber downy mildew germ, then place in the crop cultivation case and cultivate.
Condition of culture
Cultivation temperature: 28 ℃ of daytimes, 23 ℃ of nights.Incubation time: 6 days.
Investigation and method of counting
6 days " Invest, Then Investigate " control efficiency are cultivated in moisturizing in incubator, by the grade scale classification record of medicine inspecting institute of the Ministry of Agriculture " agricultural chemicals field trial criterion ", calculate control efficiency with disease index, and obtain virulence regression equation and EC
50, according to Wadley fungicide in mixture synergy evaluation assessment, calculate interactional ratio behind two single dose mixtures (be that SR＜0.5 is antagonism, 0.5≤SR≤1.5 are summation action, and SR＞1.5 are synergistic effect).
Result of the test sees the following form 2.
The joint toxicity measuring result of table 2 cyazofamid and dimethomorph
Result of the test shows: cyazofamid and dimethomorph weight ratio are 1: 2 o'clock, and its interaction ratio is respectively 1.98, and the bactericide interaction theory according to GiSi (1985) proposes has the notable synergistic effect.
The joint toxicity measuring of test example 3 cyazofamids and metalaxyl
For the examination bacterium
Cucumber downy mildew (Psenudoperonos pora.Cubensis)
For the examination cucumber variety
" the close thorn in Shandong ", cucumber is potted plant to be cultured to two open and flat phases of few leaf in the greenhouse.
The experimental concentration design
The experimental concentration of combination medicament and cyazofamid single dose is five concentration of 48,24,12,6,3 μ g/ml, and the metalaxyl concentration for the treatment of is 400,200,100,50,25 μ g/ml, and other establishes the blank of not dosing, and each is processed 4 times and repeats.
Test method
Select second true leaf of the potted plant cucumber of growth neat and consistent, put to culture dish at petiole place humidification cotton balls after cutting, by top set concentration in the crops sprayer processing of spraying, after the blade liquid dries, in second day inoculation cucumber downy mildew germ, then place in the crop cultivation case and cultivate.
Condition of culture
Cultivation temperature: 28 ℃ of daytimes, 23 ℃ of nights.Incubation time: 6 days.
Investigation and method of counting
6 days " Invest, Then Investigate " control efficiency are cultivated in moisturizing in incubator, by the grade scale classification record of medicine inspecting institute of the Ministry of Agriculture " agricultural chemicals field trial criterion ", calculate control efficiency with disease index, and obtain virulence regression equation and EC
50, according to Wadley fungicide in mixture synergy evaluation assessment, calculate interactional ratio behind two single dose mixtures (be that SR＜0.5 is antagonism, 0.5≤SR≤1.5 are summation action, and SR＞1.5 are synergistic effect).
Result of the test sees the following form 3.
The joint toxicity measuring result of table 3 cyazofamid and metalaxyl
Result of the test shows: cyazofamid and metalaxyl weight ratio are 1: 2 o'clock, and its interaction ratio is respectively 2.11, and the bactericide interaction theory according to GiSi (1985) proposes has the notable synergistic effect.
The joint toxicity measuring of test example 4 cyazofamids and white urea cyanogen
For the examination bacterium
Downy mildew of garpe (Plasmopara viticola)
For the examination grape variety
" huge peak ", grape is potted plant to be cultured to two open and flat phases of few leaf in the greenhouse.
The experimental concentration design
The experimental concentration of combination medicament single dose is five concentration of 32,16,8,4,2 μ g/ml, and cyazofamid and white urea cyanogen concentration for the treatment of are 32,16,8,4,2 μ g/ml, and other establishes the blank of not dosing, and each is processed 4 times and repeats.
Test method
Select second true leaf of the grape pot of growth neat and consistent, put to culture dish at petiole place humidification cotton balls after cutting, by top set concentration in the crops sprayer processing of spraying, after the blade liquid dries, in second day inoculation downy mildew of garpe germ, then place in the crop cultivation case and cultivate.
Condition of culture
Cultivation temperature: 28 ℃ of daytimes, 23 ℃ of nights.Incubation time: 6 days.
Investigation and method of counting
6 days " Invest, Then Investigate " control efficiency are cultivated in moisturizing in incubator, by the grade scale classification record of medicine inspecting institute of the Ministry of Agriculture " agricultural chemicals field trial criterion ", calculate control efficiency with disease index, and obtain virulence regression equation and EC
50, according to Wadley fungicide in mixture synergy evaluation assessment, calculate interactional ratio behind two single dose mixtures (be that SR＜0.5 is antagonism, 0.5≤SR≤1.5 are summation action, and SR＞1.5 are synergistic effect).
Result of the test sees the following form 4.
Table 4 cyazofamid and white urea cyanogen joint toxicity measuring result
Result of the test shows: cyazofamid and white urea cyanogen weight ratio are 1: 1 o'clock, and its interaction ratio is respectively 1.97, and the bactericide interaction theory according to GiSi (1985) proposes has the notable synergistic effect.
Test example 5 cyazofamids are Yu the joint toxicity measuring of Evil frost spirit
For the examination bacterium
Cucumber downy mildew (Psenudoperonos pora.Cubensis)
For the examination cucumber variety
" the close thorn in Shandong ", cucumber is potted plant to be cultured to two open and flat phases of few leaf in the greenhouse.
The experimental concentration design
The experimental concentration of combination medicament is five concentration of 24,12,6,3,1.5 μ g/ml, and cyazofamid is Ji the concentration for the treatment of of Evil frost spirit is 40,20,10,5,2.5 μ g/ml, and other establishes the blank of not dosing, and each is processed 4 times and repeats.
Test method
Select second true leaf of the potted plant cucumber of growth neat and consistent, put to culture dish at petiole place humidification cotton balls after cutting, by top set concentration in the crops sprayer processing of spraying, after the blade liquid dries, in second day inoculation cucumber downy mildew germ, then place in the crop cultivation case and cultivate.
Condition of culture
Cultivation temperature: 28 ℃ of daytimes, 23 ℃ of nights.Incubation time: 6 days.
Investigation and method of counting
6 days " Invest, Then Investigate " control efficiency are cultivated in moisturizing in incubator, by the grade scale classification record of medicine inspecting institute of the Ministry of Agriculture " agricultural chemicals field trial criterion ", calculate control efficiency with disease index, and obtain virulence regression equation and EC
50, according to Wadley fungicide in mixture synergy evaluation assessment, calculate interactional ratio behind two single dose mixtures (be that SR＜0.5 is antagonism, 0.5≤SR≤1.5 are summation action, and SR＞1.5 are synergistic effect).
Result of the test sees the following form 5.
Table 5 cyazofamid is Yu the joint toxicity measuring result of Evil frost spirit
Result of the test shows: cyazofamid is Yu the clever weight ratio of Evil frost is 1: 1 o'clock, and its interaction ratio is respectively 2.19, and the bactericide interaction theory according to GiSi (1985) proposes has the notable synergistic effect.
Although above the present invention is described in detail with a general description of the specific embodiments, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements all belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.
Claims (3)
1. bactericidal composition, the cyazofamid and the thiophanate-methyl that it is characterized in that containing the synergy effective dose are active component, and the weight ratio of cyazofamid and thiophanate-methyl is 1: 8～1: 12.
2. according to claim 1 formulated missible oil, microemulsion, aqueous emulsion, suspending agent, wetting powder, water dispersible granules or the granule of bactericidal composition.
3. according to claim 1 bactericidal composition is used for the purposes of downy mildew, epidemic disease and late blight that control causes by the Oomycete disease fungus.
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| CN102027957A (en) * | 2010-12-30 | 2011-04-27 | 陕西美邦农药有限公司 | Cyazofamid-containing bactericidal composition |
| CN102763644B (en) * | 2012-06-30 | 2016-02-10 | 广东中迅农科股份有限公司 | A kind of cyazofamid microemulsion and preparation method thereof |
| CN102939975B (en) * | 2012-11-21 | 2014-03-26 | 上海沪联生物药业（夏邑）股份有限公司 | Sterilization composition containing triflumizole and dimethomorph and application thereof |
| ES2685043T3 (en) * | 2013-04-16 | 2018-10-05 | Upl Limited | Fungicidal composition |
| CN105432697A (en) * | 2015-12-27 | 2016-03-30 | 胡凡营 | Biological pesticide containing cyazofamid and cymoxanil and preparation method of biological pesticide |
| CN107980780A (en) * | 2017-11-22 | 2018-05-04 | 程骏 | A kind of fungicide containing thiophanate-methyl |
| CN110558322A (en) * | 2019-10-15 | 2019-12-13 | 安徽省益农化工有限公司 | Cyazofamid-dimethomorph suspending agent and processing method thereof |
| CN115299452B (en) * | 2022-06-30 | 2024-02-27 | 陕西美邦药业集团股份有限公司 | Bactericidal composition containing oxadixyl |
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| CN101262764A (en) * | 2005-07-14 | 2008-09-10 | 巴斯夫欧洲公司 | Fungicide mixtures based on 1-methyl-pyrazol-4-yl carboxylic acid anilides |
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