CN101755798A - Bactericide composition containing fluazinam - Google Patents

Bactericide composition containing fluazinam Download PDF

Info

Publication number
CN101755798A
CN101755798A CN200910223992A CN200910223992A CN101755798A CN 101755798 A CN101755798 A CN 101755798A CN 200910223992 A CN200910223992 A CN 200910223992A CN 200910223992 A CN200910223992 A CN 200910223992A CN 101755798 A CN101755798 A CN 101755798A
Authority
CN
China
Prior art keywords
fluazinam
weight ratio
amine
bactericidal composition
enestroburin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN200910223992A
Other languages
Chinese (zh)
Inventor
陈章艳
游文莉
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
XINNONG DAZHENG BIOLOGICAL ENGINEERING Co Ltd FUJIAN
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CN200910223992A priority Critical patent/CN101755798A/en
Publication of CN101755798A publication Critical patent/CN101755798A/en
Pending legal-status Critical Current

Links

Landscapes

  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention relates to a bactericide composition, in particular to a bactericide composition which comprises synergistically effective amount of fluazinam and any one of bactericidal active ingredients selected from the group consisting of dimethomorph, enestroburin, cymoxanil, fluopicolide, mandipropamid and flumorph.

Description

The bactericidal composition that contains fluazinam
Technical field
The present invention relates to a kind of Synergistic bactericidal composition, particularly relate to a kind of Synergistic bactericidal composition that comprises fluazinam and be selected from arbitrary fungicide active ingredient of dimethomorph, Enestroburin, white urea cyanogen, fluorine pyrrole bacterium amine, mandipropamid amine and flumorph
Background technology
Fluazinam (fluazinam), chemical name are 3-chloro-N-(3-chloro-5-trifluoromethyl-2-pyridine radicals)-α, α, and α-three fluoro-2,6-dinitro para-totuidine is the pyridyl amine compound of ICI agrochemicals company exploitation.Fluazinam is a kind of efficient, wide spectrum, novel bactericide.The mechanism of action and characteristics: resist the course of infection in all stages by inhibitor spore germination, mycelia breakthrough, growth and sporulation.The fungicidal spectrum of fluazinam is very wide, and its effect is better than the GPF (General Protection False bactericide, to interlink spore genus, Botrytis, Phytophthora, Plasmopara, Sclerotinia with black to cultivate the Pseudomonas bacterium very effective.
Dimethomorph (dimethomorph), (E, Z)-4-[3-(4-chlorphenyl)-3-(3, the 4-Dimethoxyphenyl) acryloyl] morpholine, belong to the morpholine series bactericidal agent, be known systemic fungicide, its effect characteristics are to destroy the formation of cell envelope, all there is effect in each stage to oomycetes history of life, and is particularly responsive in the formation stage of sporangiophore and egg spore, and Peronospora and Phytophthora fungi are had preventive effect preferably.
Enestroburin (enestroburin) is to be the novel agrochemical of lead compound exploitation with the natural antibiotics, belongs to methoxy acrylic bactericide.Its action principle is to suppress the mitochondrial breathing of fungi, by cytochrome b c 1The Q of complex 0The position in conjunction with suppressing mitochondrial current potential transmission, the germ energy is synthetic to play bactericidal action thereby destroy.
Frost urea cyanogen (cymoxanil); 2-cyano group-N-[(ethylamino-) carbonyl]-2-(methoxyl group imido grpup) acetamide; it is a kind of high-efficiency low-toxicity bactericide; belong to the substituted urea class bactericide; have contact and local systemic action, can suppress spore germination, use with to prolong the lasting period with protective fungicide; be widely used in brassicaceous vegetable such as cucumber, grape, tomato, lichee, huge dish and tobacco etc. and go up the Peronosporales fungi, belong to as epidemic disease frost genus, Peronospora, single shaft frost.
Fluorine pyrrole bacterium amine (fluopicolide); 2; 6-two chloro-N-[(3-chloro-5-trifluoromethyl-2-pyridine radicals) methyl] benzamide; be the benzamides wide-spectrum bactericide; Oomycete fungi germ there is very high biologically active; have protection and therapeutic action, act on cell membrane and intercellular specific proteins and show bactericidal activity.Fluorine pyrrole bacterium amine has stronger permeability; can permeate downwards from the blade upper surface, conduct to the blade tip direction, can protect blade not to be subjected to infection process after young shoot is handled from phyllopodium; can also distribute from root along the plant xylem to whole strain crop, but can not conduct along phloem.
Mandipropamid amine (mandipropamid), 2-(4-chlorphenyl)-N-[2-(3-methoxyl group-4-(2-third alkynyloxy group)-phenyl)-ethyl group]-2-(2-third alkynyloxy group)-acetamide, be acid amide fungicides.Its mechanism of action is for suppressing the biosynthesis of phosphatide; leaf portion and fruit disease that exhausted big number is caused by oomycetes all have good preventive effect; the spore that is in the sprouting stage had higher activity; and can suppress mycelia and grow up and sporulation; can be absorbed rapidly by blade; and rest in the leaf table wax coat, blade is shielded.
Flumorph (flumorph); 4-[3-(4-fluorophenyl)-3-(3; the 4-Dimethoxyphenyl) acryloyl] morpholine; be the acryloyl morpholine series bactericidal agent; the inhibitory action that sporangium is sprouted is remarkable; protection and therapeutic action are had both, and are widely used in the control of various crop disease as disinfectant use in agriculture, especially Peronospora, Phytophthora germ are had special efficacy.
The inventor studies have shown that by a series of, fluazinam is mixed with the arbitrary fungicide active ingredient that is selected from dimethomorph, Enestroburin, white urea cyanogen, fluorine pyrrole bacterium amine, mandipropamid amine and flumorph the target bacterium is had extremely strong synergistic effect, improve bactericidal activity greatly, thereby finished the present invention.
Summary of the invention
The object of the present invention is to provide a kind of mixed ratio reasonable, control efficiency is good, the Synergistic bactericidal composition that drug cost is low.
Another object of the present invention is to provide by formulations such as the formulated missible oil of this bactericidal composition, microemulsion, aqueous emulsion, suspending agent, wetting powder, water dispersible granules, granules.
Another object of the present invention is to provide the purposes that bactericidal composition is used to prevent and treat the downy mildew that causes by the Oomycete disease fungus, eqpidemic disease, late blight.
Technical scheme of the present invention is as described below:
A kind of bactericidal composition, the arbitrary fungicide active ingredient that it is characterized in that containing the fluazinam of synergy effective dose and be selected from dimethomorph, Enestroburin, white urea cyanogen, fluorine pyrrole bacterium amine, mandipropamid amine and flumorph.
Wherein, fluazinam can change in very wide scope with the weight ratio that is selected from arbitrary fungicide active ingredient of dimethomorph, Enestroburin, white urea cyanogen, fluorine pyrrole bacterium amine, mandipropamid amine and flumorph, be preferably 40: 1～1: 40, more preferably 15: 1～1: 10.
And, the weight ratio of fluazinam and dimethomorph more preferably 10: 1～1: 1; The weight ratio of fluazinam and Enestroburin or mandipropamid amine more preferably 5: 1～1: 1; The weight ratio of fluazinam and white urea cyanogen more preferably 12: 1～3: 1; The weight ratio of fluazinam and fluorine pyrrole bacterium amine more preferably 15: 1～8: 1; The weight ratio of fluazinam and flumorph more preferably 8: 1～3: 1.
Active component is 0.5% to 95% of a gross weight in the composition of the present invention.
Except the fungicidal activity composition, also can comprise functional aid in the bactericidal composition of the present invention.That is to say that the present composition can add other components such as functional aid, be mixed with the preparation of missible oil, aqueous emulsion, microemulsion, suspending agent, wetting powder, water dispersible granules or other formulations.
In these preparations, except that active component, all contain surfactant, and also can contain thinners such as organic solvent or cosolvent, carrier (filler) or water, add other functional aids such as antifreeze, thickener, stabilizing agent, defoamer, disintegrant in case of necessity according to different dosage form.Surfactant can be emulsifier, dispersant, wetting agent or bleeding agent.Surfactant can be nonionic with ionic, also can be two or more compound uses.
Described emulsifier comprises alkylphenol-polyethenoxy (polyoxypropylene) ether, benzylphenol APEO, phenethyl phenol polyethenoxy ether, fatty alcohol-polyoxyethylene ether, polyoxyethylene carboxylate, phenethyl phenol polyethenoxy polyethenoxy ether, castor oil ethylene oxide adduct, alkylphenol polyoxyethylene formaldehyde condensation products, and the phosphate of these compounds, alkyl benzene sulphonate (calcium salt or sodium salt) or directly use commercially available emulsifier monomer and composite product.Concrete example is as farming breast 500 #Series, 600 #Series, 1600 #Series, NP is serial, OP is serial, AEO is serial, 700 #, 400 #Serial and composite kind 2201,0203B, 2021B, 4103 etc., these all are emulsifier productions well known in the art, market is on sale.
Described dispersant; wetting agent; bleeding agent comprises lignosulfonates (sodium salt or calcium salt); Negel; condensation compound of methyl naphthalene sulfonic acid and formaldehyde sulphate; naphthalene sulfonic acid-formaldehyde condensation product; methanonaphthalene sodium sulfonate dibutyl naphthalene sulfonic acid-formaldehyde condensation product; N-methyl fatty acyl group sodium taurocholate; alkylphenol polyoxyethylene formaldehyde condensation products sulphate; polymerization of carboxylic acid sodium; the alkylphenol polyoxyethylene phosphate; the withered powder of tea; saponin; lignosulfite; aliphatic alcohol sulfate; dodecyl sodium sulfate; alkylbenzenesulfonate; penetrant t; bleeding agent OT; fatty acid amide-N methyl taurine sodium salt; sodium sulfate of polyethenoxy ether of fatty alcohol; draw back powder; JFC; can use separately, also can compoundly use.Above-mentioned surfactant all is materials well known in the art, can obtain by various commercial channel.
Described solvent, cosolvent comprise toluene, dimethylbenzene, chlorobenzene, solvent naphtha, alpha-methyl-naphthalene, turpentine oil, carrene, chloroform, methyl alcohol, ethanol, isopropyl alcohol, butanols, isoamyl alcohol, cyclohexanone, isophorone, acetophenone, ethyl acetate, butyl acetate, N.N-dimethyl formamide, dimethyl sulfoxide (DMSO), methyl cellosolve, ethyl cellosolve etc.
Described antifreeze comprises ethylene glycol, glycerine, propane diols, urea, glycerine, ether diglycol, polyethylene glycol, isopropyl alcohol etc.
Described carrier, filler comprise white carbon, precipitated calcium carbonate, potter's clay, attapulgite, kaolin, diatomite, bentonite, sepiolite, zeolite, quartz sand, talcum powder, can use separately, also can two kinds or above mixing use.
Described disintegrant has sodium alginate, sodium carboxymethyl starch, sulfuric acid amine, bentonite, urea, calcium chloride.
Described defoamer has bubble enemy, silicone, C 8-10Fatty alcohol, C 10-20Saturated fat acids (as capric acid, stearic acid etc.) and amide-type.
Described binding agent comprises starch, dextrin, gum Arabic, soybean protein, gelatine, sodium sulphate, gypsum, polyvinyl alcohol, polyethylene glycol, rosin, paraffin, CMC, sodium silicate.Can use separately, also can two or morely use with.
Can contain toner in the preparation, inorganic pigment iron oxide for example, titanium oxide or Prussian blue; Organic dyestuff is as A Lizhalin, azo dyes, metal phthalocyanine or kiton colors.In water-base preparation, for preventing moldy metamorphism, need to add a certain amount of preservative, described preservative has Sodium Benzoate, polyformaldehyde, sorbitol, formalin, citric acid, to the yl benzoic acid methyl esters, face phenyl phenol sodium, 1,2-benzisothiazole-3-ketone (code name BIT) etc.
The product that the present invention describes can be the finished product preparation form to be provided, and promptly each material mixes in the composition, but the composition of composition also can provide directly mixing in bucket (jar) before using with independent preparation.Concentrate of the present invention mixes the concentration that obtains required active substance usually with water.
Composition of the present invention can be used for various plants, as Chinese cabbage, wild cabbage, cauliflower, wax gourd, cucumber, tomato, lichee, grape, wheat, rape, potato, tobacco and flowers etc.The place of using of the product that composition of the present invention and carrier are made is farmland, orchard or warehouse etc.
Composition of the present invention also can with other compound with weeding, desinsection or bactericidal property particularly the protective fungicide agent composition use, also can use with nematocide, protective agent, growth regulator, nutrient for plants or soil conditioner mixture.Composition of the present invention can be used by common method, as cast, injection, spraying.Amount of application of the present invention is with weather condition or crop state variation, and time of application can be before or after crop be susceptible.The duration of protective effect is usually relevant with the content of individualized compound in the composition, also relevant with extraneous factor, weather for example, but by using suitable formulation can slow down climatic influences.
The remarkable advantage of composition of the present invention: the one, composition shows extremely strong synergistic effect in certain ratio range, the bactericidal effect of mixed composition is significantly improved than its single agent, thereby reduced using dosage, when reducing peasant's drug cost, reduced influence degree.The 2nd, interracial structure differs bigger, and the resistance that the application of said composition can delay single agent takes place and development.
Embodiment:
The present invention is elaborated with following specific embodiment, so that advantage of the present invention is more obvious, but the present invention is limited to these examples absolutely not.Percentage all is weight percentage in the prescription of all preparations, and the processing of biologically-active moiety is effective content.
One, preparation embodiment:
1, the preparation of missible oil
By the prescription requirement, add solvent, former medicine, emulsifier respectively, mix, use the hot bath heating for dissolving in case of necessity, promptly obtain transparence missible oil.As embodiment 1～3 prescription.
Embodiment 1 (15% missible oil)
Fluazinam 10%, Enestroburin 5%, farming breast 500 #7.5%, agricultural newborn 600-2 #4.5%, methyl alcohol 8%, dimethylbenzene complements to 100%.
Embodiment 2 (15% missible oil)
Fluazinam 10%, mandipropamid amine 5%, farming breast 507 #10%, agricultural newborn OX-653 3%, methyl alcohol 10%, dimethylbenzene complements to 100%.
Embodiment 3 (20% missible oil)
Fluazinam 17%, flumorph 3%, farming breast 500 #10%, agricultural newborn OX-635 5%, methyl alcohol 10%, dimethylbenzene complements to 100%.
2, the preparation of wetting powder
By the prescription requirement, former medicine, various auxiliary agent and filler etc. are fully mixed, after ultrafine crusher is pulverized, promptly obtain converted products.As embodiment 4～9 prescriptions.
Embodiment 4 (30% wetting powder)
Fluazinam 25%, dimethomorph 5%, lauryl sodium sulfate 1.5%, carboxymethyl cellulose 1%, sodium lignin sulfonate 10%, precipitated calcium carbonate complements to 100%.
Embodiment 5 (15% wetting powder)
Fluazinam 10%, Enestroburin 5%, lauryl sodium sulfate 1.5%, carboxymethyl cellulose 1%, sodium lignin sulfonate 10%, precipitated calcium carbonate complements to 100%.
Embodiment 6 (40% wetting powder)
Fluazinam 35%, white urea cyanogen 5%, neopelex 1%, white carbon 10%, sodium lignin sulfonate 10%, precipitated calcium carbonate complements to 100%.
Embodiment 7 (30% wetting powder)
Fluazinam 27%, fluorine pyrrole bacterium amine 3%, neopelex 1%, white carbon 10%, sodium lignin sulfonate 10%, precipitated calcium carbonate complements to 100%.
Embodiment 8 (15% wetting powder)
Fluazinam 10%, mandipropamid amine 5%, alkyl amide taurate 2%, Fluhyzon formaldehyde condensation 5%, alkylphenol-polyethenoxy phosphate 5%, precipitated calcium carbonate complements to 100%.
Embodiment 9 (20% wetting powder)
Fluazinam 17%, flumorph 3%, neopelex 1%, white carbon 10%, naphtholsulfonic acid formaldehyde condensation products sodium salt 10%, precipitated calcium carbonate complements to 100%.
3, the preparation of aqueous emulsion
Former medicine, solvent, emulsifier added with co-emulsifier be in the same place, make dissolving be even oil phase.Water, antifreeze etc. is mixed, becomes the homogeneous water.Under high-speed stirred, water is joined oil phase or oil phase is joined water, form the aqueous emulsion of favorable dispersibility.As embodiment 10～12 prescriptions.
Embodiment 10 (15% aqueous emulsion)
Fluazinam 12%, dimethomorph 3%, polyvinyl alcohol 0.8%, in triphenylethylene phenol+oxirane, phosphorylation, the triethanolamine and 8.5%, alkyl-diethylene glycol ether-sodium sulfonate 2.5%, farming breast 2,201 13%, dimethyl formamide 8%, ethylene glycol 5%, water complements to 100%.
Embodiment 11 (25% aqueous emulsion)
Fluazinam 22%, white urea cyanogen 3%, polyvinyl alcohol 0.9%, alkylaryl polyoxyethylene propylene ether 8.5%, farming breast 2,201 15%, dimethyl formamide 12%, ethylene glycol 5%, water complements to 100%.
Embodiment 12 (30% aqueous emulsion)
Fluazinam 27%, fluorine pyrrole bacterium amine 3%, polyvinyl alcohol 1.5%, alkylaryl polyoxyethylene propylene ether 10%, farming breast 220115%, dimethyl formamide 15%, ethylene glycol 5%, water complements to 100%.
4, the preparation of water-dispersible granules
Former medicine and powder carrier, wetting spreader and binding agent etc. are mixed pulverizing, after adding water again and mediating, add in the comminutor that the certain specification screen cloth is housed and carry out granulation.And then drying, screening (pressing the screen cloth scope) promptly gets granular product.As embodiment 13～14 prescriptions.
Embodiment 13 (35% water-dispersible granules)
Fluazinam 29%, dimethomorph 6%, methyl naphthalene sulfonate formaldehyde condensate 12%, epoxidized polyether 5%, soluble starch 15%, gypsum complements to 100%.
Embodiment 14 (35% water-dispersible granules)
Fluazinam 30%, flumorph 5%, methanonaphthalene sodium sulfonate 5%, epoxidized polyether 5%, sodium sulphate, gypsum complements to 100%.
5, the preparation of suspending agent
By the prescription requirement, be medium with water, former medicine, dispersant, suspending agent and antifreezing agent etc. are added in the sand milling still, carry out porphyrize, make suspending agent.As embodiment 15～17 prescriptions.
Embodiment 15 (40% suspending agent)
Fluazinam 35%, white urea cyanogen 5%, methyl naphthalene sulfonate formaldehyde condensate 5.5%, white carbon 0.2%, aluminium-magnesium silicate 0.18%, ethylene glycol 4%, silicone a little, water complements to 100%.
Embodiment 16 (30% suspending agent)
Fluazinam 27%, fluorine pyrrole bacterium amine 3%, calcium lignosulfonate 5%, white carbon 0.3%, ethylene glycol 4%, silicone a little, water complements to 100%.
Embodiment 17 (30% suspending agent)
Fluazinam 20%, mandipropamid amine 10%, methyl naphthalene sulfonic acid naphthaldehyde condensation compound 2.5%, bentonite 1.0%, farming breast 16013%, glycerine 4%, silicone a little, water complements to 100%.
6, the preparation of microemulsion
Former medicine, solvent, emulsifier added with co-emulsifier be in the same place, make dissolving be even oil phase.Water, antifreeze etc. is mixed, becomes the homogeneous water.Under high-speed stirred, water is joined oil phase or oil phase is joined water, form the microemulsion of favorable dispersibility.As embodiment 18～20 prescriptions.
Embodiment 18 (15% microemulsion)
Fluazinam 10%, Enestroburin 5%, propyl alcohol 7%, phenethyl phenol polyethenoxy ether 10%, carrene 10%, glycerine 5%, water complements to 100%.
Embodiment 19 (30% microemulsion)
Fluazinam 27%, fluorine pyrrole bacterium amine 3%, dimethylbenzene 12%, phenyl xenol polyvinylether 15%, ring ethanol 8%, ethylene glycol 8%, water complements to 100%.
Embodiment 20 (15% microemulsion)
Fluazinam 10%, mandipropamid amine 5%, ethanol 6%, sodium alkyl benzene sulfonate 8%, the third hexanone 8%, propane diols 6%, water complements to 100%.
Two, biological Examples
The joint toxicity measuring of test example 1 fluazinam and dimethomorph
For the examination bacterium
Cucumber downy mildew (Psenudoperonos pora.Cubensis)
For the examination cucumber variety
" the close thorn in Shandong ", cucumber is potted plant to be cultured to two open and flat phases of few leaf in the greenhouse.
The experimental concentration design
The experimental concentration of combination medicament is five concentration of 20,10,5,2.5,1.25 μ g/ml, the fluazinam concentration of treatment is 32,16,8,4,2 μ g/ml, the dimethomorph concentration of treatment is 40,20,10,5,2.5 μ g/ml, and other establishes the blank of not dosing, and each is handled 4 times and repeats.
Test method
Select second true leaf of the potted plant cucumber of growth neat and consistent, cutting the back puts to culture dish at petiole place humidification cotton balls, by the processing of on the crop sprayer, spraying of top set concentration, treat that the blade soup dries the back in second day inoculation cucumber downy mildew germ, places then in the crop cultivation case and cultivates.
Condition of culture
Cultivation temperature: 28 ℃ of daytimes, 23 ℃ of nights.Incubation time: 6 days.
Investigation and method of counting
In incubator, preserve moisture and cultivate 6 days " Invest, Then Investigate " control efficiency,, calculate control efficiency with disease index, and obtain virulence regression equation and EC by the grade scale classification record of medicine inspecting institute of the Ministry of Agriculture " agricultural chemicals field trial criterion " 50,, calculate interactional ratio behind two single agent compositions (be that SR＜0.5 is antagonism, 0.5≤SR≤1.5 are summation action, and SR＞1.5 are synergistic effect) according to Wadley bactericide mixture synergy evaluation assessment.
Result of the test sees the following form 1.
Table 1 fluazinam and dimethomorph joint toxicity measuring result
Figure G2009102239923D00071
Figure G2009102239923D00081
Result of the test shows; Fluazinam and dimethomorph weight ratio are 5: 1 o'clock, and its interaction ratio is respectively 1.95, and the bactericide interaction theory according to GiSi (1985) proposes has the notable synergistic effect.
Test example 2 fluazinams and Enestroburin joint toxicity measuring
For the examination bacterium
Potato late blight (Phytophthora infestans de Bary)
For examination potato kind
No. 7, potato " open ", potato is potted plant to be cultured to two open and flat phases of few leaf in the greenhouse.
The experimental concentration design
The experimental concentration of combination medicament and the agent of Enestroburin list is five concentration of 20,10,5,2.5,1.25 μ g/ml, and the fluazinam concentration of treatment is 40,20,10,5,2.5 μ g/ml, and other establishes the blank of not dosing, and each is handled 4 times and repeats.
Test method
Select second true leaf of the potted plant potato of growth neat and consistent, cutting the back puts to culture dish at petiole place humidification cotton balls, by the processing of on the crop sprayer, spraying of top set concentration, treat that the blade soup dries the back in second day inoculation potato late blight germ, places then in the crop cultivation case and cultivates.
Condition of culture
Cultivation temperature: 28 ℃ of daytimes, 23 ℃ of nights.Incubation time: 6 days.
Investigation and method of counting
In incubator, preserve moisture and cultivate 6 days " Invest, Then Investigate " control efficiency,, calculate control efficiency with disease index, and obtain virulence regression equation and EC by the grade scale classification record of medicine inspecting institute of the Ministry of Agriculture " agricultural chemicals field trial criterion " 50,, calculate interactional ratio behind two single agent compositions (be that SR＜0.5 is antagonism, 0.5≤SR≤1.5 are summation action, and SR＞1.5 are synergistic effect) according to Wadley bactericide mixture synergy evaluation assessment.
Result of the test sees the following form 2.
Table 2 fluazinam and Enestroburin joint toxicity measuring result
Figure G2009102239923D00082
Figure G2009102239923D00091
Result of the test shows; Fluazinam, Enestroburin weight ratio are 2: 1 o'clock, and its interaction ratio is respectively 1.95, and the bactericide interaction theory according to GiSi (1985) proposes has the notable synergistic effect.
Test example 3 fluazinams and white urea cyanogen joint toxicity measuring
For the examination bacterium
Downy mildew of garpe (Plasmopara viticola)
For the examination grape variety
" huge cutting edge of a knife or a sword ", grape is potted plant to be cultured to two open and flat phases of few leaf in the greenhouse.
The experimental concentration design
The experimental concentration of combination medicament and the agent of fluazinam list is five concentration of 20,10,5,2.5,1.25 μ g/ml, and white urea cyanogen concentration of treatment is 40,20,10,5,2.5 μ g/ml, and other establishes the blank of not dosing, and each is handled 4 times and repeats.
Test method
Select second true leaf of the grape pot of growth neat and consistent, cutting the back puts to culture dish at petiole place humidification cotton balls, by the processing of on the crop sprayer, spraying of top set concentration, treat that the blade soup dries the back in second day inoculation downy mildew of garpe germ, places then in the crop cultivation case and cultivates.
Condition of culture
Cultivation temperature: 28 ℃ of daytimes, 23 ℃ of nights.Incubation time: 6 days.
Investigation and method of counting
In incubator, preserve moisture and cultivate 6 days " Invest, Then Investigate " control efficiency,, calculate control efficiency with disease index, and obtain virulence regression equation and EC by the grade scale classification record of medicine inspecting institute of the Ministry of Agriculture " agricultural chemicals field trial criterion " 50,, calculate interactional ratio behind two single agent compositions (be that SR＜0.5 is antagonism, 0.5≤SR≤1.5 are summation action, and SR＞1.5 are synergistic effect) according to Wadley bactericide mixture synergy evaluation assessment.
Result of the test sees the following form 3.
Table 3 fluazinam and white urea cyanogen joint toxicity measuring result
Figure G2009102239923D00092
Figure G2009102239923D00101
Result of the test shows: fluazinam and white urea cyanogen weight ratio are 7: 1 o'clock, and its interaction ratio is respectively 2.05, and the bactericide interaction theory according to GiSi (1985) proposes has the notable synergistic effect.
Test example 4 fluazinams and fluorine pyrrole bacterium amine joint toxicity measuring
For the examination bacterium
Cucumber downy mildew (Psenudoperonos pora.Cubensis)
For the examination cucumber variety
" the close thorn in Shandong ", cucumber is potted plant to be cultured to two open and flat phases of few leaf in the greenhouse.
The experimental concentration design
The experimental concentration of combination medicament and the agent of fluorine pyrrole bacterium amine list is five concentration of 16,8,4,2,1 μ g/ml, and the fluazinam concentration of treatment is 32,16,8,4,2 μ g/ml, and other establishes the blank of not dosing, and each is handled 4 times and repeats.
Test method
Select second true leaf of the potted plant cucumber of growth neat and consistent, cutting the back puts to culture dish at petiole place humidification cotton balls, by the processing of on the crop sprayer, spraying of top set concentration, treat that the blade soup dries the back in second day inoculation cucumber downy mildew germ, places then in the crop cultivation case and cultivates.
Condition of culture
Cultivation temperature: 28 ℃ of daytimes, 23 ℃ of nights.Incubation time: 6 days.
Investigation and method of counting
In incubator, preserve moisture and cultivate 6 days " Invest, Then Investigate " control efficiency,, calculate control efficiency with disease index, and obtain virulence regression equation and EC by the grade scale classification record of medicine inspecting institute of the Ministry of Agriculture " agricultural chemicals field trial criterion " 50,, calculate interactional ratio behind two single agent compositions (be that SR＜0.5 is antagonism, 0.5≤SR≤1.5 are summation action, and SR＞1.5 are synergistic effect) according to Wadley bactericide mixture synergy evaluation assessment.
Result of the test sees the following form 4.
The joint toxicity measuring result of table 4 fluazinam and fluorine pyrrole bacterium amine
Figure G2009102239923D00111
Result of the test shows: fluazinam and fluorine pyrrole bacterium amine weight ratio are 10: 1 o'clock, and its interaction ratio is respectively 1.98, and the bactericide interaction theory according to GiSi (1985) proposes has the notable synergistic effect.
Test example 5 fluazinams and mandipropamid amine joint toxicity measuring
For the examination bacterium
Capsicum epidemic disease (Phytophthora capsici Leonian)
For the examination capsicum variety
" rich green pepper No. ", capsicum is potted plant to be cultured to two open and flat phases of few leaf in the greenhouse.
The experimental concentration design
The experimental concentration of combination medicament and the agent of mandipropamid amine list is five concentration of 20,10,5,2.5,1.25 μ g/ml, and the fluazinam concentration of treatment is 40,20,10,5,2.5 μ g/ml, and other establishes the blank of not dosing, and each is handled 4 times and repeats.
Test method
Select second true leaf of the potted plant capsicum of growth neat and consistent, cutting the back puts to culture dish at petiole place humidification cotton balls, by the processing of on the crop sprayer, spraying of top set concentration, treat that the blade soup dries the back in second day inoculation capsicum epidemic disease germ, places then in the crop cultivation case and cultivates.
Condition of culture
Cultivation temperature: 28 ℃ of daytimes, 23 ℃ of nights.Incubation time: 6 days.
Investigation and method of counting
In incubator, preserve moisture and cultivate 6 days " Invest, Then Investigate " control efficiency,, calculate control efficiency with disease index, and obtain virulence regression equation and EC by the grade scale classification record of medicine inspecting institute of the Ministry of Agriculture " agricultural chemicals field trial criterion " 50,, calculate interactional ratio behind two single agent compositions (be that SR＜0.5 is antagonism, 0.5≤SR≤1.5 are summation action, and SR＞1.5 are synergistic effect) according to Wadley bactericide mixture synergy evaluation assessment.
Result of the test sees the following form 5.
The joint toxicity measuring result of table 5 fluazinam and mandipropamid amine
Figure G2009102239923D00121
Result of the test shows; Fluazinam and mandipropamid amine weight ratio are 2: 1 o'clock, and its interaction ratio is respectively 1.98, and the bactericide interaction theory according to GiSi (1985) proposes has the notable synergistic effect.
The joint toxicity measuring of test example 6 fluazinams and flumorph
For the examination bacterium
Downy mildew of garpe (Plasmopara viticola)
For the examination grape variety
" huge peak ", grape is potted plant to be cultured to two open and flat phases of few leaf in the greenhouse.
The experimental concentration design
The experimental concentration of combination medicament and the agent of fluazinam list is five concentration of 20,10,5,2.5,1.25 μ g/ml, and the flumorph concentration of treatment is 40,20,10,5,2.5 μ g/ml, and other establishes the blank of not dosing, and each is handled 4 times and repeats.
Test method
Select second true leaf of the grape pot of growth neat and consistent, cutting the back puts to culture dish at petiole place humidification cotton balls, by the processing of on the crop sprayer, spraying of top set concentration, treat that the blade soup dries the back in second day inoculation downy mildew of garpe germ, places then in the crop cultivation case and cultivates.
Condition of culture
Cultivation temperature: 28 ℃ of daytimes, 23 ℃ of nights.Incubation time: 6 days.
Investigation and method of counting
In incubator, preserve moisture and cultivate 6 days " Invest, Then Investigate " control efficiency,, calculate control efficiency with disease index, and obtain virulence regression equation and EC by the grade scale classification record of medicine inspecting institute of the Ministry of Agriculture " agricultural chemicals field trial criterion " 50,, calculate interactional ratio behind two single agent compositions (be that SR＜0.5 is antagonism, 0.5≤SR≤1.5 are summation action, and SR＞1.5 are synergistic effect) according to Wadley bactericide mixture synergy evaluation assessment.
Result of the test sees the following form 6.
The joint toxicity measuring result of table 6 fluazinam and flumorph
Figure G2009102239923D00131
Result of the test shows; Fluazinam and flumorph weight ratio are 6: 1 o'clock, and its interaction ratio is respectively 1.86, and the bactericide interaction theory according to GiSi (1985) proposes has the notable synergistic effect.
Though above the present invention is described in detail with a general description of the specific embodiments, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements all belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.

Claims (10)

1. bactericidal composition is characterized in that containing the fluazinam and the arbitrary fungicide active ingredient that is selected from dimethomorph, Enestroburin, white urea cyanogen, fluorine pyrrole bacterium amine, mandipropamid amine and flumorph of synergy effective dose.
2. according to the bactericidal composition of claim 1, it is characterized in that fluazinam and the weight ratio that is selected from arbitrary fungicide active ingredient of dimethomorph, Enestroburin, white urea cyanogen, fluorine pyrrole bacterium amine, mandipropamid amine and flumorph be preferably 40: 1～1: 40.
3. according to the bactericidal composition of claim 1-2, it is characterized in that fluazinam and the weight ratio that is selected from arbitrary fungicide active ingredient of dimethomorph, Enestroburin, white urea cyanogen, fluorine pyrrole bacterium amine, mandipropamid amine and flumorph be preferably 15: 1～1: 10.
4. according to the bactericidal composition of claim 1-3, it is characterized in that the weight ratio more preferably 10: 1～1: 1 of fluazinam and dimethomorph.
5. according to the bactericidal composition of claim 1-3, it is characterized in that the weight ratio more preferably 5: 1～1: 1 of fluazinam and Enestroburin or mandipropamid amine.
6. according to the bactericidal composition of claim 1-3, it is characterized in that the weight ratio more preferably 12: 1～3: 1 of fluazinam and white urea cyanogen.
7. according to the composition pesticide of claim 1-3, it is characterized in that the weight ratio more preferably 15: 1～8: 1 of fluazinam and fluorine pyrrole bacterium amine.
8. according to the composition pesticide of claim 1-3, it is characterized in that the weight ratio more preferably 8: 1～3: 1 of fluazinam and flumorph.
9. missible oil, microemulsion, aqueous emulsion, suspending agent, wetting powder, water dispersible granules or the granule formulated according to the described bactericidal composition of arbitrary claim of claim 1-8.
10. be used to prevent and treat the purposes of the downy mildew, eqpidemic disease and the late blight that cause by the Oomycete disease fungus according to the described bactericidal composition of arbitrary claim of claim 1-8.
CN200910223992A 2009-11-23 2009-11-23 Bactericide composition containing fluazinam Pending CN101755798A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN200910223992A CN101755798A (en) 2009-11-23 2009-11-23 Bactericide composition containing fluazinam

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN200910223992A CN101755798A (en) 2009-11-23 2009-11-23 Bactericide composition containing fluazinam

Publications (1)

Publication Number Publication Date
CN101755798A true CN101755798A (en) 2010-06-30

Family

ID=42487303

Family Applications (1)

Application Number Title Priority Date Filing Date
CN200910223992A Pending CN101755798A (en) 2009-11-23 2009-11-23 Bactericide composition containing fluazinam

Country Status (1)

Country Link
CN (1) CN101755798A (en)

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2713754A2 (en) 2011-05-25 2014-04-09 Ishihara Sangyo Kaisha, Ltd. Agricultural or horticultural fungicide composition and method for controlling plant pathogen
CN104381266A (en) * 2014-09-25 2015-03-04 北京燕化永乐生物科技股份有限公司 Sterilization composition
CN104604866A (en) * 2013-11-05 2015-05-13 南京华洲药业有限公司 Sterilization composition containing fluazinam and fluopicolide, and applications thereof
CN105076139A (en) * 2015-09-06 2015-11-25 安徽出入境检验检疫局检验检疫技术中心 Bactericidal composition containing fluopyram and fluazinam
CN106035380A (en) * 2016-06-27 2016-10-26 中国烟草总公司郑州烟草研究院 Bactericide composition containing benzamide and dinitroaniline bactericides and biocontrol bacterium, preparation and applications
CN107183026A (en) * 2017-06-13 2017-09-22 江苏省绿盾植保农药实验有限公司 A kind of composition pesticide and its application containing Enestroburin and fluazinam
EP3292761A1 (en) * 2011-03-23 2018-03-14 Bayer Intellectual Property GmbH Active compound combinations
CN110637825A (en) * 2019-10-31 2020-01-03 湖南省植物保护研究所 Bactericidal composition containing cymoxanil and fluazinam and application of bactericidal composition in prevention and treatment of cucumber downy mildew
CN113598183A (en) * 2021-08-04 2021-11-05 兴农药业（中国）有限公司 Fluopicolide and fluazinam mixed bactericide suspension, preparation method and application

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3292761A1 (en) * 2011-03-23 2018-03-14 Bayer Intellectual Property GmbH Active compound combinations
US9167815B2 (en) 2011-05-25 2015-10-27 Ishihara Sangyo Kaisha, Ltd. Agricultural or horticultural fungicide composition and method for controlling plant pathogen
AU2012259756B2 (en) * 2011-05-25 2015-04-09 Ishihara Sangyo Kaisha, Ltd. Agricultural or horticultural fungicide composition and method for controlling plant pathogen
EP2713754A2 (en) 2011-05-25 2014-04-09 Ishihara Sangyo Kaisha, Ltd. Agricultural or horticultural fungicide composition and method for controlling plant pathogen
CN104604866A (en) * 2013-11-05 2015-05-13 南京华洲药业有限公司 Sterilization composition containing fluazinam and fluopicolide, and applications thereof
WO2015067010A1 (en) * 2013-11-05 2015-05-14 南京华洲药业有限公司 Bactericide composition containing fluazinam and fluopicolide and application thereof
CN104381266A (en) * 2014-09-25 2015-03-04 北京燕化永乐生物科技股份有限公司 Sterilization composition
CN105076139A (en) * 2015-09-06 2015-11-25 安徽出入境检验检疫局检验检疫技术中心 Bactericidal composition containing fluopyram and fluazinam
CN106035380A (en) * 2016-06-27 2016-10-26 中国烟草总公司郑州烟草研究院 Bactericide composition containing benzamide and dinitroaniline bactericides and biocontrol bacterium, preparation and applications
CN107183026A (en) * 2017-06-13 2017-09-22 江苏省绿盾植保农药实验有限公司 A kind of composition pesticide and its application containing Enestroburin and fluazinam
CN107183026B (en) * 2017-06-13 2019-09-27 江苏丘陵地区镇江农业科学研究所 A kind of composition pesticide and its application containing Enestroburin and fluazinam
CN110637825A (en) * 2019-10-31 2020-01-03 湖南省植物保护研究所 Bactericidal composition containing cymoxanil and fluazinam and application of bactericidal composition in prevention and treatment of cucumber downy mildew
CN113598183A (en) * 2021-08-04 2021-11-05 兴农药业（中国）有限公司 Fluopicolide and fluazinam mixed bactericide suspension, preparation method and application

Similar Documents

Publication Publication Date Title
CN101755798A (en) Bactericide composition containing fluazinam
CN101755825B (en) Bactericide composition containing thifluzamide
CN101755799B (en) Cyazofamid-containing bactericidal composition
CN101755797A (en) Bactericide composition containing boscalid
CN101755845B (en) Bactericide composition containing flutolanil
CN101999356B (en) Sterilization composition containing fluopicolide and metalaxyl and application thereof
CN101755785B (en) Bactericide composition containing famoxadone
CN102696645A (en) Pesticide composition containing pyraclostrobin and metalaxyl
CN102197818A (en) Bactericidal composition
CN101779677A (en) Sterilization composition containing penconazole
CN101755782A (en) Bactericide composition containing cyprodinil
CN102217616B (en) Fluopicolpkd-containing bactericidal composition
CN101715775B (en) Sterilizing composition containing ethirimol
CN101715771B (en) Sterilizing composition containing imibenconazole
CN101366386A (en) Agricultural chemical composition based on Pymetrozine
CN101743983B (en) Fungicidal composition containing flumorph
CN101433199A (en) Bactericidal composition
CN101755796B (en) Bactericide composition containing flumorph
CN101755800B (en) Bactericide composition containing iprovalicarb
CN102715173B (en) Bactericidal composition containing phenylate diazole and fluoxastrobin and application of bactericidal composition
CN103392715A (en) Sterilization composition containing cyprodinil and captan
CN1311746C (en) Bactericidal compositions of flumorph and enostroburin and synergist
CN101779675A (en) Sterilization composition containing difenoconazole
CN106982837A (en) A kind of Pesticidal combination containing fluorine cyanogen insect amide and its application
CN103348990B (en) Bactericidal composition containing mandipropamid and captan

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
ASS Succession or assignment of patent right

Free format text: FORMER OWNER: YOU WENLI

Owner name: XINNONG DAZHENG BIOLOGICAL ENGINEERING CO., LTD.,

Free format text: FORMER OWNER: CHEN ZHANGYAN

Effective date: 20110426

C41 Transfer of patent application or patent right or utility model
C53 Correction of patent for invention or patent application
CB03 Change of inventor or designer information

Inventor after: Chen Weimin

Inventor after: Chen Fan

Inventor before: Chen Zhangyan

Inventor before: You Wenli

COR Change of bibliographic data

Free format text: CORRECT: INVENTOR; FROM: CHEN ZHANGYAN YOU WENLI TO: CHEN WEIMIN CHEN FAN

Free format text: CORRECT: ADDRESS; FROM: 350002 FUJIAN SINO-DASHING BIOLOGICAL ENGINEERING CO., LTD., 6/F, TAIYANGCHENG BUILDING, NO. 268, YANGQIAO WEST ROAD, FUZHOU CITY, FUJIAN PROVINCE TO: 350002 UNIT 6F, BUILDING 2, TAIYANGCHENG COMPREHENSIVE BUILDING, NO. 268, YANGQIAO WEST ROAD, FUZHOU CITY, FUJIAN PROVINCE

TA01 Transfer of patent application right

Effective date of registration: 20110426

Address after: Unit 6F No. 2 Yangqiao road Fuzhou Fujian province 350002 City 268 Sun City Building

Applicant after: Xinnong Dazheng Biological Engineering Co., Ltd., Fujian

Address before: Fuzhou City, Fujian province 350002 Yangqiao Road No. 268, Solar City Tower Rio de Janeiro, 6 floor Fujian sinodashing Bioengineering Co. Ltd.

Applicant before: Chen Zhangyan

Co-applicant before: You Wenli

C02 Deemed withdrawal of patent application after publication (patent law 2001)
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20100630