CN101779677A - Sterilization composition containing penconazole - Google Patents

Sterilization composition containing penconazole Download PDF

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Publication number
CN101779677A
CN101779677A CN200910259850A CN200910259850A CN101779677A CN 101779677 A CN101779677 A CN 101779677A CN 200910259850 A CN200910259850 A CN 200910259850A CN 200910259850 A CN200910259850 A CN 200910259850A CN 101779677 A CN101779677 A CN 101779677A
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penconazole
spore
mancozeb
propineb
tpn
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陈章艳
游文莉
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XINNONG DAZHENG BIOLOGICAL ENGINEERING Co Ltd FUJIAN
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Abstract

The invention relates to a synergistic sterilization composition, particularly to a synergistic sterilization composition containing penconazole with increased effective quantity and any one sterilization active ingredient selected from azoxystrobin, fernasan, mancozeb, sperlox, propineb, chlorothalonil and wuyiencin.

Description

The bactericidal composition that contains penconazole
Technical field
The present invention relates to a kind of Synergistic bactericidal composition, particularly relate to a kind of penconazole and Synergistic bactericidal composition that is selected from arbitrary fungicide active ingredient of Fluoxastrobin, tmtd, mancozeb, zineb, Propineb, tpn and Wuyiencin of comprising.
Background technology
Penconazole (penconazole), 1-[2-(2, the 4-dichlorophenyl) amyl group]-1-hydrogen-1,2, the 4-triazole belongs to triazole bactericidal agent, the main mechanism of action is sterol demethylation inhibitor, destroy and stop the biosynthesis of the cell membrane important composition composition ergosterol of germ, cause cell membrane not form, make germ death.Because absorption in having well, therefore can be promptly by plant absorbing, and in conducted inside; Have good protection and therapeutic activity; the disease that fungi (especially Erysiphaceae Venturia and other spore Gammaproteobacterias that causes a disease, Basidiomycetes etc.) is caused has preventive and therapeutic effect, and is remarkably productive to the pathogen of eliminating melon and fruit, vegetables, coffee, ornamental plants etc.
Fluoxastrobin (azoxystrobin); (E)-and 2-{2-[6-(2-cyano-benzene oxygen) pyrimidine-4-base oxygen base] phenyl }-the 3-methoxy-methyl acrylate; methoxy acrylic bactericide for Syngenta Co.,Ltd's exploitation; the mitochondrial respiratory inhibitor; promptly by shifting to the electronics of cromoci at cytochrome B cl; thereby suppress mitochondrial breathing, have protection, root out, infiltration, systemic activity, suppress spore germination and mycelial growth and produce spore always.
Tmtd (thiram); tetramethyl thiuram disulfide; belong to organosulfureous fungicide; it is a kind of bactericide of tool protective effect; its has a broad antifungal spectrum is mainly used in and handles seed and soil, the seedling blight of control cereal class smut and various crop; also can be used for spraying, prevent and treat some fruit trees, vegetable disease.
Mancozeb (mancozeb); the polycomponent coordination compound of ethylidene dithiocarbamate manganese and zinc salt; belong to organic sulfur class protective fungicide; fungicidal spectrum is wider; it mainly is the oxidation that suppresses pyruvic acid in the thalline; effective to multiple diseases such as the anthracnose on fruit tree, the vegetables, early blight, simultaneously its normal and systemic fungicide is mixed, and is used for the generation of delaying drug resistance.
Zineb (zineb); zinc ethylene bisdithiocarbamate; belong to organic sulfur class protective fungicide; the active ingredient chemical property is more active; in water, easily be oxidized to different thiocyanation compound; to the cause of disease thalline contain-enzyme of SH base has had strong inhibitory effects; and the direct kill pathogens spore of energy; suppress the germination of spore; stop in the germ invaded plants body; but very little to the mycelial lethal effect of cause of disease in the invaded plants body, many germs such as Pseudoperonospora cubensis, late disease bacteria and anthrax bacteria etc. there are strong action of contace poison.
Propineb (propineb), acrylic bisdithiocarbamic zinc belongs to dithiocarbamates, acts on the synthetic of fungal cell wall and protein, can suppress infecting and sprouting of spore, can suppress mycelial growth simultaneously, causes its distortion, death.And this medicine contains and is easy to the zinc element that absorbed by crop, the quality that helps promoting plant growth and improve fruit.
Tpn (chlorothalonil), daconil M (2,4; 5,6-tetrachloro-1,3-benzene dicarbonitrile); belong to organochlorine class protective fungicide, can with the combined with protein of cysteine in the glyceraldehyde 3-phosphate dehydro-genase among the fungal cell, destroy the metabolism of cell and loss of life power.It mainly acts on is the prevention fungal infection, inhales conduction in not having, but at plant surface good adhesivity is arranged, and is not subject to rain drop erosion, and the long drug effect phase is arranged.
Wuyiencin; it is a kind of streptomycete series bactericidal agent; belong to low toxicity, efficient, wide spectrum and the strong sterilization antibiotic medicament of interior absorption; have protection and therapeutic action; can suppress the synthetic of pathogen protein, and suppress pathogen thalline mycelial growth, sporulation, sprouting and influence the somatic cells membrane permeability.Can carry out induction of resistance to plant, all plants all have the certain basis resistance to pathogen, and the various plants disease fungus is had stronger inhibitory action.
The inventor studies have shown that by a series of, penconazole is mixed with the arbitrary fungicide active ingredient that is selected from Fluoxastrobin, tmtd, mancozeb, zineb, Propineb, tpn and Wuyiencin the target bacterium is had extremely strong synergistic effect, improve bactericidal activity greatly, thereby finished the present invention.
Summary of the invention
The object of the present invention is to provide a kind of mixed ratio reasonable, control efficiency is good, the Synergistic bactericidal composition that drug cost is low.
Another object of the present invention is to provide by formulations such as the formulated missible oil of this bactericidal composition, microemulsion, aqueous emulsion, suspending agent, aqua, wetting powder, water dispersible granules, granules.
Another object of the present invention is to provide the purposes that this bactericidal composition is used to prevent and treat powdery mildew that sac fungi, imperfect fungus cause, alternaria leaf spot of apple, ring rot of apple, pear scab etc.
Technical scheme of the present invention is as described below:
A kind of bactericidal composition, the arbitrary fungicide active ingredient that it is characterized in that containing the penconazole of synergy effective dose and be selected from Fluoxastrobin, tmtd, mancozeb, zineb, Propineb, tpn and Wuyiencin.
Wherein, penconazole can change in very wide scope with the weight ratio that is selected from arbitrary fungicide active ingredient of Fluoxastrobin, tmtd, mancozeb, zineb, Propineb, tpn and Wuyiencin, be preferably 20: 1～1: 40, more preferably 10: 1～1: 40.
And, the weight ratio of penconazole and Fluoxastrobin more preferably 3: 1～1: 10; The weight ratio of penconazole and tmtd, mancozeb, zineb, Propineb or tpn more preferably 1: 1～1: 40; The weight ratio of penconazole and Wuyiencin more preferably 5: 1～1: 5.
Active component is 0.5% to 95% of a gross weight in the composition of the present invention.
Except the fungicidal activity composition, also can comprise functional aid in the bactericidal composition of the present invention.That is to say that the present composition can add other components such as functional aid, be mixed with the preparation of missible oil, aqueous emulsion, microemulsion, suspending agent, aqua, wetting powder, water dispersible granules or other formulations.
In these preparations, except that active component, all contain surfactant, and also can contain thinners such as organic solvent or cosolvent, carrier (filler) or water, add other functional aids such as antifreeze, thickener, stabilizing agent, defoamer, disintegrant in case of necessity according to different dosage form.Surfactant can be emulsifier, dispersant, wetting agent or bleeding agent.Surfactant can be nonionic with ionic, also can be two or more compound uses.
Described emulsifier comprises alkylphenol-polyethenoxy (polyoxypropylene) ether, benzylphenol APEO, phenethyl phenol polyethenoxy ether, fatty alcohol-polyoxyethylene ether, polyoxyethylene carboxylate, phenethyl phenol polyethenoxy polyethenoxy ether, castor oil ethylene oxide adduct, alkylphenol polyoxyethylene formaldehyde condensation products, and the phosphate of these compounds, alkyl benzene sulphonate (calcium salt or sodium salt) or directly use commercially available emulsifier monomer and composite product.Concrete example is as farming breast 500 #Series, 600 #Series, 1600 #Series, NP is serial, OP is serial, AEO is serial, 700 #, 400 #Serial and composite kind 2201,0203B, 2021B, 4103 etc., these all are emulsifier productions well known in the art, market is on sale.
Described dispersant; wetting agent; bleeding agent comprises lignosulfonates (sodium salt or calcium salt); Negel; condensation compound of methyl naphthalene sulfonic acid and formaldehyde sulphate; naphthalene sulfonic acid-formaldehyde condensation product; methanonaphthalene sodium sulfonate dibutyl naphthalene sulfonic acid-formaldehyde condensation product; N-methyl fatty acyl group sodium taurocholate; alkylphenol polyoxyethylene formaldehyde condensation products sulphate; polymerization of carboxylic acid sodium; the alkylphenol polyoxyethylene phosphate; the withered powder of tea; saponin; lignosulfite; aliphatic alcohol sulfate; dodecyl sodium sulfate; alkylbenzenesulfonate; penetrant t; bleeding agent OT; fatty acid amide-N methyl taurine sodium salt; sodium sulfate of polyethenoxy ether of fatty alcohol; draw back powder; JFC; can use separately, also can compoundly use.Above-mentioned surfactant all is materials well known in the art, can obtain by various commercial channel.
Described solvent, cosolvent comprise toluene, dimethylbenzene, chlorobenzene, solvent naphtha, alpha-methyl-naphthalene, turpentine oil, carrene, chloroform, methyl alcohol, ethanol, isopropyl alcohol, butanols, isoamyl alcohol, cyclohexanone, isophorone, acetophenone, ethyl acetate, butyl acetate, N.N-dimethyl formamide, dimethyl sulfoxide (DMSO), methyl cellosolve, ethyl cellosolve etc.
Described antifreeze is ethylene glycol, glycerine, propane diols, urea, glycerine, ether diglycol, polyethylene glycol, isopropyl alcohol etc.
Described carrier, filler comprise white carbon, precipitated calcium carbonate, potter's clay, attapulgite, kaolin, diatomite, bentonite, sepiolite, zeolite, quartz sand, talcum powder, can use separately, also can two kinds or above mixing use.
Described disintegrant has sodium alginate, sodium carboxymethyl starch, sulfuric acid amine, bentonite, urea, calcium chloride.
Described defoamer has bubble enemy, silicone, C 8-10Fatty alcohol, C 10-20Saturated fat acids (as capric acid, stearic acid etc.) and amide-type.
Described binding agent comprises starch, dextrin, gum Arabic, soybean protein, gelatine, sodium sulphate, gypsum, polyvinyl alcohol, polyethylene glycol, rosin, paraffin, CMC, sodium silicate.Can use separately, also can two or morely use with.
Can contain toner in the preparation, inorganic pigment iron oxide for example, titanium oxide or Prussian blue; Organic dyestuff is as A Lizhalin, azo dyes, metal phthalocyanine or kiton colors.In water-base preparation, for preventing moldy metamorphism, need to add a certain amount of preservative, described preservative has Sodium Benzoate, polyformaldehyde, sorbitol, formalin, citric acid, to the yl benzoic acid methyl esters, face phenyl phenol sodium, 1,2-benzisothiazole-3-ketone (code name BIT) etc.
The product that the present invention describes can be the finished product preparation form to be provided, and promptly each material mixes in the composition, but the composition of composition also can provide directly mixing in bucket (jar) before using with independent preparation.Concentrate of the present invention mixes the concentration that obtains required active substance usually with water.
Composition of the present invention can be used for paddy rice.The place of using of the product that composition of the present invention and carrier are made is a paddy field etc.
Composition of the present invention also can with other compound with weeding, desinsection or bactericidal property particularly the protective fungicide agent composition use, also can use with nematocide, protective agent, growth regulator, nutrient for plants or soil conditioner mixture.Composition of the present invention can be used by common method, as cast, injection, spraying.Amount of application of the present invention is with weather condition or crop state variation, and time of application can be before or after crop be susceptible.The duration of protective effect is usually relevant with the content of individualized compound in the composition, also relevant with extraneous factor, weather for example, but by using suitable formulation can slow down climatic influences.
The remarkable advantage of composition of the present invention: the one, composition shows extremely strong synergistic effect in certain ratio range, the bactericidal effect of mixed composition is significantly improved than its single agent, thereby reduced using dosage, when reducing peasant's drug cost, reduced influence degree.The 2nd, interracial structure differs bigger, and the resistance that the application of said composition can delay single agent takes place and development.
Embodiment:
The present invention is elaborated with following specific embodiment, so that advantage of the present invention is more obvious, but the present invention is limited to these examples absolutely not.Percentage all is weight percentage in the prescription of all preparations, and the processing of biologically-active moiety is effective content.
One, preparation embodiment:
1, the preparation of missible oil
By the prescription requirement, add solvent, former medicine, emulsifier respectively, mix, use the hot bath heating for dissolving in case of necessity, promptly obtain transparence missible oil.As embodiment 1～3 prescription.
Embodiment 1 (30% missible oil)
Penconazole 5%, tmtd 25%, farming breast 500 #7.5%, agricultural newborn 600-2 #4.5%, methyl alcohol 8%, dimethylbenzene complements to 100%.
Embodiment 2 (35% missible oil)
Penconazole 5%, mancozeb 30%, farming breast 507 #10%, agricultural newborn OX-653 3%, methyl alcohol 10%, dimethylbenzene complements to 100%.
Embodiment 3 (35% missible oil)
Penconazole 3%, tpn 32%, farming breast 500 #10%, agricultural newborn OX-635 5%, methyl alcohol 10%, dimethylbenzene complements to 100%.
2, the preparation of wetting powder
By the prescription requirement, former medicine, various auxiliary agent and filler etc. are fully mixed, after ultrafine crusher is pulverized, promptly obtain converted products.As embodiment 4～9 prescriptions.
Embodiment 4 (35% wetting powder)
Penconazole 5%, Fluoxastrobin 30%, lauryl sodium sulfate 1.5%, carboxymethyl cellulose 1%, sodium lignin sulfonate 10%, precipitated calcium carbonate complements to 100%.
Embodiment 5 (45% wetting powder)
Penconazole 6%, tmtd 39%, lauryl sodium sulfate 1.5%, carboxymethyl cellulose 1%, sodium lignin sulfonate 10%, precipitated calcium carbonate complements to 100%.
Embodiment 6 (50% wetting powder)
Penconazole 6%, mancozeb 44%, neopelex 1%, white carbon 10%, sodium lignin sulfonate 10%, precipitated calcium carbonate complements to 100%.
Embodiment 7 (50% wetting powder)
Penconazole 8%, Propineb 42%, neopelex 1%, white carbon 10%, sodium lignin sulfonate 10%, precipitated calcium carbonate complements to 100%.
Embodiment 8 (55% wetting powder)
Penconazole 5%, tpn 50%, alkyl amide taurate 2%, Fluhyzon formaldehyde condensation 5%, alkylphenol-polyethenoxy phosphate 5%, precipitated calcium carbonate complements to 100%.
Embodiment 9 (10% wetting powder)
Penconazole 8%, Wuyiencin 2%, neopelex 1%, white carbon 10%, naphtholsulfonic acid formaldehyde condensation products sodium salt 10%, precipitated calcium carbonate complements to 100%.
3, the preparation of aqueous emulsion
Former medicine, solvent, emulsifier added with co-emulsifier be in the same place, make dissolving be even oil phase.Water, antifreeze etc. is mixed, becomes the homogeneous water.Under high-speed stirred, water is joined oil phase or oil phase is joined water, form the aqueous emulsion of favorable dispersibility.As embodiment 10～12 prescriptions.
Embodiment 10 (25% aqueous emulsion)
Penconazole 4%, tmtd 21%, polyvinyl alcohol 0.8%, in triphenylethylene phenol+oxirane, phosphorylation, the triethanolamine and 8.5%, alkyl-diethylene glycol ether-sodium sulfonate 2.5%, farming breast 220113%, dimethyl formamide 8%, ethylene glycol 5%, water complements to 100%.
Embodiment 11 (25% aqueous emulsion)
Penconazole 4%, mancozeb 21%, polyvinyl alcohol 0.9%, alkylaryl polyoxyethylene propylene ether 8.5%, farming breast 220115%, dimethyl formamide 12%, ethylene glycol 5%, water complements to 100%.
Embodiment 12 (35% aqueous emulsion)
Penconazole 3%, tpn 32%, polyvinyl alcohol 1.5%, alkylaryl polyoxyethylene propylene ether 10%, farming breast 2,201 15%, dimethyl formamide 15%, ethylene glycol 5%, water complements to 100%.
4, the preparation of water-dispersible granules
Former medicine and powder carrier, wetting spreader and binding agent etc. are mixed pulverizing, after adding water again and mediating, add in the comminutor that the certain specification screen cloth is housed and carry out granulation.And then drying, screening (pressing the screen cloth scope) promptly gets granular product.As embodiment 13～14 prescriptions.
Embodiment 13 (15% water-dispersible granules)
Penconazole 7%, Fluoxastrobin 8%, methyl naphthalene sulfonate formaldehyde condensate 12%, epoxidized polyether 5%, soluble starch 15%, gypsum complements to 100%.
Embodiment 14 (50% water-dispersible granules)
Penconazole 8%, Propineb 42%, methanonaphthalene sodium sulfonate 5%, epoxidized polyether 5%, sodium sulphate, gypsum complements to 100%.
5, the preparation of suspending agent
By the prescription requirement, be medium with water, former medicine, dispersant, suspending agent and antifreezing agent etc. are added in the sand milling still, carry out porphyrize, make suspending agent.As embodiment 15～17 prescriptions.
Embodiment 15 (30% suspending agent)
Penconazole 8%, Fluoxastrobin 22%, methyl naphthalene sulfonate formaldehyde condensate 5.5%, white carbon 0.2%, aluminium-magnesium silicate 0.18%, ethylene glycol 4%, silicone a little, water complements to 100%.
Embodiment 16 (50% suspending agent)
Penconazole 8%, zineb 42%, calcium lignosulfonate 5%, white carbon 0.3%, ethylene glycol 4%, silicone a little, water complements to 100%.
Embodiment 17 (55% suspending agent)
Penconazole 5%, tpn 50%, methyl naphthalene sulfonic acid naphthaldehyde condensation compound 2.5%, bentonite 1.0%, farming breast 1,601 3%, glycerine 4%, silicone a little, water complements to 100%.
6, the preparation of microemulsion
Former medicine, solvent, emulsifier added with co-emulsifier be in the same place, make dissolving be even oil phase.Water, antifreeze etc. is mixed, becomes the homogeneous water.Under high-speed stirred, water is joined oil phase or oil phase is joined water, form the microemulsion of favorable dispersibility.As embodiment 18～20 prescriptions.
Embodiment 18 (25% microemulsion)
Penconazole 4%, tmtd 21%, propyl alcohol 7%, phenethyl phenol polyethenoxy ether 10%, carrene 10%, glycerine 5%, water complements to 100%.
Embodiment 19 (30% microemulsion)
Penconazole 3%, mancozeb 27%, dimethylbenzene 12%, phenyl xenol polyvinylether 15%, ring ethanol 8%, ethylene glycol 8%, water complements to 100%.
Embodiment 20 (35% microemulsion)
Penconazole 3%, tpn 32%, ethanol 6%, sodium alkyl benzene sulfonate 8%, the third hexanone 8%, propane diols 6%, water complements to 100%.
7, the preparation of aqua
By the prescription requirement, required solvent is injected reaction pot, add all pesticide original medicines then, stir and make its dissolving, add auxiliary agent more successively, emulsifier, last first alcohol and water is made aqua.As embodiment 21 prescriptions.
Embodiment 21 (5% aqua)
Penconazole 3%, Wuyiencin 2%, dimethyl formamide 10%, Gao Jinsai ketone 1.5～6%, fatty alcohol-polyoxyethylene ether 5%, alkylphenol polyoxyethylene 5%, fatty diglycollic amide 5%, sulfonic acid 2%, alcohol or water are mended to 100%.
Two, biological Examples:
The joint toxicity measuring of test example 1 penconazole and Fluoxastrobin
For the examination bacterium
Wheat powdery mildew (Blumeria graminis (DC.) Speer)
For the examination cucumber variety
" raise wheat 158 ", wheat is potted plant to be cultured to two open and flat phases of few leaf in the greenhouse.
The experimental concentration design
The experimental concentration of penconazole list agent is five concentration of 6.4,3.2,1.6,0.8,0.4 μ g/ml, Fluoxastrobin list agent concentration of treatment is 20,10,5,2.5,1.25 μ g/ml, five concentration gradients are also established in the test of combination medicament, and other establishes the blank of not dosing, and each is handled 4 times and repeats.
Test method
With the pure water that is added with low quantity of surfactant (as Tween-80 etc.), wash and get the fresh spore that covers with on the white powder germ plant leaf blade, filter with double gauze, making spore concentration is 1 * 10 5The suspension of individual spore/mL, standby.Soup is evenly sprayed in the blade face to all wetting, treat behind the soup natural air drying standby.Use the spore suspension spray inoculation.24h inoculation before chemicals treatment.Postvaccinal examination material natural air drying moves to thermostatic chamber then, cultivates 7d～10d under the condition of 20 ℃～24 ℃ of temperature.
Investigation and method of counting
By medicine inspecting institute of the Ministry of Agriculture " agricultural chemicals indoor bioassay test rule " method, treat that the sick leaf rate of blank reaches 80% when above, the classification investigation state of an illness condition of getting a haircut everywhere, 30 leaves are investigated in every processing at least, and grade scale is: 0 grade: anosis;
1 grade: lesion area accounts for below 5% of one-piece blade area;
3 grades: lesion area accounts for 5%～15% of one-piece blade area;
5 grades: lesion area accounts for 15%～25% of one-piece blade area;
7 grades: lesion area accounts for 25%～50% of one-piece blade area;
9 grades: lesion area accounts for 50%～75% of one-piece blade area;
11 grades: lesion area accounts for more than 75% of one-piece blade area.According to survey data, calculate disease index and control efficiency that each is handled.Do regression analysis according to each drug concentration logarithm value and preventive effect probability value, calculate the EC of each medicament 50,, calculate interactional ratio behind two single agent compositions (be that SR＜0.5 is antagonism, 0.5≤SR≤1.5 are summation action, and SR＞1.5 are synergistic effect) according to Wadley bactericide mixture synergy evaluation assessment.
Result of the test sees the following form 1.
The joint toxicity measuring result of table 1. penconazole and Fluoxastrobin
Figure G2009102598502D00081
Result of the test shows: the weight ratio of penconazole and Fluoxastrobin is 1: 5 o'clock, and its interaction ratio is respectively 2.03, and the bactericide interaction theory according to GiSi (1985) proposes has the notable synergistic effect.
The joint toxicity measuring of test example 2 penconazoles and tmtd
For the examination bacterium
Wheat powdery mildew (Blumeria graminis (DC.) Speer)
For the examination cucumber variety
" raise wheat 158 ", wheat is potted plant to be cultured to two open and flat phases of few leaf in the greenhouse.
The experimental concentration design
Penconazole list agent concentration of treatment is 4,2,1,0.5,0.25 μ g/ml, and tmtd list agent concentration of treatment is 64,32,16,8,4 μ g/ml, and five concentration gradients are also established in the test of combination medicament, and other establishes the blank of not dosing, and each is handled 4 times and repeats.
Test method
With the pure water that is added with low quantity of surfactant (as Tween-80 etc.), wash and get the fresh spore that covers with on the white powder germ plant leaf blade, filter with double gauze, making spore concentration is 1 * 10 5The suspension of individual spore/mL, standby.Soup is evenly sprayed in the blade face to all wetting, treat behind the soup natural air drying standby.Use the spore suspension spray inoculation.24h inoculation before chemicals treatment.Postvaccinal examination material natural air drying moves to thermostatic chamber then, cultivates 7d～10d under the condition of 20 ℃～24 ℃ of temperature.
Investigation and method of counting
By medicine inspecting institute of the Ministry of Agriculture " agricultural chemicals indoor bioassay test rule " method, treat that the sick leaf rate of blank reaches 80% when above, the classification investigation state of an illness condition of getting a haircut everywhere, 30 leaves are investigated in every processing at least, and grade scale is: 0 grade: anosis;
1 grade: lesion area accounts for below 5% of one-piece blade area;
3 grades: lesion area accounts for 5%～15% of one-piece blade area;
5 grades: lesion area accounts for 15%～25% of one-piece blade area;
7 grades: lesion area accounts for 25%～50% of one-piece blade area;
9 grades: lesion area accounts for 50%～75% of one-piece blade area;
11 grades: lesion area accounts for more than 75% of one-piece blade area.According to survey data, calculate disease index and control efficiency that each is handled.Do regression analysis according to each drug concentration logarithm value and preventive effect probability value, calculate the EC of each medicament 50,, calculate interactional ratio behind two single agent compositions (be that SR＜0.5 is antagonism, 0.5≤SR≤1.5 are summation action, and SR＞1.5 are synergistic effect) according to Wadley bactericide mixture synergy evaluation assessment.
Result of the test sees the following form 2.
The joint toxicity measuring result of table 2. penconazole and tmtd
Figure G2009102598502D00091
Result of the test shows: the weight ratio of penconazole and tmtd is 1: 20 o'clock, and its interaction ratio is respectively 1.89, and the bactericide interaction theory according to GiSi (1985) proposes has the notable synergistic effect.
The joint toxicity measuring of test example 3 penconazoles and mancozeb
For the examination bacterium
Pear scab (Venturia pirina Anderh)
The experimental concentration design
Penconazole list agent concentration of treatment is 4,2,1,0.5,0.25 μ g/ml, and mancozeb list agent concentration of treatment is 32,16,8,4,2 μ g/ml, and five concentration gradients are also established in the test of combination medicament, and other establishes the blank of not dosing, and each is handled 4 times and repeats.
Test method
With deionized water wash-out, filtration on medium or the diseased tissues, centrifugal (1000r/min) 5min removes supernatant, adds deionized water, and is centrifugal again with cultured disease fungus spore.At last with deionization with the spore resuspension to every milliliter 1 * 10 5Individual～1 * 10 7Individual spore, and add 0.5% glucose solution., from the low concentration to the high concentration, draw soup 0.5mL successively and add respectively in the small test tube with pipette or pipettor, draw the spore suspension 0.5mL for preparing then, make soup and spore suspension mixed in equal amounts even.Draw above-mentioned mixed liquor with micro sample adding appliance and drip on the concave slide, frame is put in the culture dish that has shallow seated groundwater then, and adding a cover preserves moisture is incubated in the incubator of preference temperature.
Investigation and method of counting
By medicine inspecting institute of the Ministry of Agriculture " agricultural chemicals indoor bioassay test rule " method, when the blank spore germination rate reaches 90% when above, the spore germination situation is respectively handled in inspection.Every processing repeats 3 above visuals field of random observation, many 200 of investigation spore sum, and number and spore sum sprouted in record respectively.Spore germ tube length is considered as sprouting greater than the short radius of spore.According to survey data, calculate the spore germination relative inhibition that each is handled.Probability value according to each drug concentration logarithm value and corresponding spore germination relative inhibition is done regression analysis, calculates the EC of each medicament 50,, calculate interactional ratio behind two single agent compositions (be that SR＜0.5 is antagonism, 0.5≤SR≤1.5 are summation action, and SR＞1.5 are synergistic effect) according to Wadley bactericide mixture synergy evaluation assessment.
Result of the test sees the following form 3.
The joint toxicity measuring result of table 3. penconazole and mancozeb
Figure G2009102598502D00101
Figure G2009102598502D00111
Result of the test shows: the weight ratio of penconazole and mancozeb is 1: 30 o'clock, and its interaction ratio is respectively 2.18, and the bactericide interaction theory according to GiSi (1985) proposes has the notable synergistic effect.
The joint toxicity measuring of test example 4 penconazoles and zineb
For the examination bacterium
Alternaria leaf spot of apple (Alternaria mali Roberts)
The experimental concentration design
Penconazole list agent concentration of treatment is 2.4,1.2,0.6,0.3,0.15 μ g/ml, zineb list agent concentration of treatment is 32,16,8,4,2 μ g/ml, five concentration gradients are also established in the test of combination medicament, and other establishes the blank of not dosing, and each is handled 4 times and repeats.
Test method
With deionized water wash-out, filtration on medium or the diseased tissues, centrifugal (1000r/min) 5min removes supernatant, adds deionized water, and is centrifugal again with cultured disease fungus spore.At last with deionization with the spore resuspension to every milliliter 1 * 10 5Individual～1 * 10 7Individual spore, and add 0.5% glucose solution., from the low concentration to the high concentration, draw soup 0.5mL successively and add respectively in the small test tube with pipette or pipettor, draw the spore suspension 0.5mL for preparing then, make soup and spore suspension mixed in equal amounts even.Draw above-mentioned mixed liquor with micro sample adding appliance and drip on the concave slide, frame is put in the culture dish that has shallow seated groundwater then, and adding a cover preserves moisture is incubated in the incubator of preference temperature.
Investigation and method of counting
By medicine inspecting institute of the Ministry of Agriculture " agricultural chemicals indoor bioassay test rule " method, when the blank spore germination rate reaches 90% when above, the spore germination situation is respectively handled in inspection.Every processing repeats 3 above visuals field of random observation, many 200 of investigation spore sum, and number and spore sum sprouted in record respectively.Spore germ tube length is considered as sprouting greater than the short radius of spore.According to survey data, calculate the spore germination relative inhibition that each is handled.Probability value according to each drug concentration logarithm value and corresponding spore germination relative inhibition is done regression analysis, calculates the EC of each medicament 50,, calculate interactional ratio behind two single agent compositions (be that SR＜0.5 is antagonism, 0.5≤SR≤1.5 are summation action, and SR＞1.5 are synergistic effect) according to Wadley bactericide mixture synergy evaluation assessment.
Result of the test sees the following form 4.
The joint toxicity measuring result of table 4. penconazole and zineb
Figure G2009102598502D00121
Result of the test shows: the weight ratio of penconazole and zineb is 1: 20 o'clock, and its interaction ratio is respectively 2.05, and the bactericide interaction theory according to GiSi (1985) proposes has the notable synergistic effect.
The joint toxicity measuring of test example 5 penconazoles and Propineb
For the examination bacterium
Alternaria leaf spot of apple (Alternaria mali Roberts)
The experimental concentration design
Penconazole list agent concentration of treatment is 2.4,1.2,0.6,0.3,0.15 μ g/ml, Propineb list agent concentration of treatment is 32,16,8,4,2 μ g/ml, five concentration gradients are also established in the test of combination medicament, and other establishes the blank of not dosing, and each is handled 4 times and repeats.
Test method
With deionized water wash-out, filtration on medium or the diseased tissues, centrifugal (1000r/min) 5min removes supernatant, adds deionized water, and is centrifugal again with cultured disease fungus spore.At last with deionization with the spore resuspension to every milliliter 1 * 10 5Individual～1 * 10 7Individual spore, and add 0.5% glucose solution., from the low concentration to the high concentration, draw soup 0.5mL successively and add respectively in the small test tube with pipette or pipettor, draw the spore suspension 0.5mL for preparing then, make soup and spore suspension mixed in equal amounts even.Draw above-mentioned mixed liquor with micro sample adding appliance and drip on the concave slide, frame is put in the culture dish that has shallow seated groundwater then, and adding a cover preserves moisture is incubated in the incubator of preference temperature.
Investigation and method of counting
By medicine inspecting institute of the Ministry of Agriculture " agricultural chemicals indoor bioassay test rule " method, when the blank spore germination rate reaches 90% when above, the spore germination situation is respectively handled in inspection.Every processing repeats 3 above visuals field of random observation, many 200 of investigation spore sum, and number and spore sum sprouted in record respectively.Spore germ tube length is considered as sprouting greater than the short radius of spore.According to survey data, calculate the spore germination relative inhibition that each is handled.Probability value according to each drug concentration logarithm value and corresponding spore germination relative inhibition is done regression analysis, calculates the EC of each medicament 50,, calculate interactional ratio behind two single agent compositions (be that SR＜0.5 is antagonism, 0.5≤SR≤1.5 are summation action, and SR＞1.5 are synergistic effect) according to Wadley bactericide mixture synergy evaluation assessment.
Result of the test sees the following form 5.
The joint toxicity measuring result of table 5. penconazole and Propineb
Result of the test shows: the weight ratio of penconazole and Propineb is 1: 15 o'clock, and its interaction ratio is respectively 2.02, and the bactericide interaction theory according to GiSi (1985) proposes has the notable synergistic effect.
The joint toxicity measuring of test example 6 penconazoles and tpn
For the examination bacterium
Grape powdery mildew (Uncinula necater (Schw.) Burr)
For the examination cucumber variety
" huge peak ", grape is potted plant to be cultured to two open and flat phases of few leaf in the greenhouse.
The experimental concentration design
Penconazole list agent concentration of treatment is 4,2,1,0.5,0.25 μ g/ml, and tpn list agent concentration of treatment is 80,40,20,10,5 μ g/ml, and five concentration gradients are also established in the test of combination medicament, and other establishes the blank of not dosing, and each is handled 4 times and repeats.
Test method
With the pure water that is added with low quantity of surfactant (as Tween-80 etc.), wash and get the fresh spore that covers with on the white powder germ plant leaf blade, filter with double gauze, making spore concentration is 1 * 10 5The suspension of individual spore/mL, standby.Soup is evenly sprayed in the blade face to all wetting, treat behind the soup natural air drying standby.Use the spore suspension spray inoculation.24h inoculation before chemicals treatment.Postvaccinal examination material natural air drying moves to thermostatic chamber then, cultivates 7d～10d under the condition of 20 ℃～24 ℃ of temperature.
Investigation and method of counting
By medicine inspecting institute of the Ministry of Agriculture " agricultural chemicals indoor bioassay test rule " method, treat that the sick leaf rate of blank reaches 80% when above, the classification investigation state of an illness condition of getting a haircut everywhere, 30 leaves are investigated in every processing at least, and grade scale is: 0 grade: anosis;
1 grade: lesion area accounts for below 5% of one-piece blade area;
3 grades: lesion area accounts for 5%～15% of one-piece blade area;
5 grades: lesion area accounts for 15%～25% of one-piece blade area;
7 grades: lesion area accounts for 25%～50% of one-piece blade area;
9 grades: lesion area accounts for 50%～75% of one-piece blade area;
11 grades: lesion area accounts for more than 75% of one-piece blade area.According to survey data, calculate disease index and control efficiency that each is handled.Do regression analysis according to each drug concentration logarithm value and preventive effect probability value, calculate the EC of each medicament 50,, calculate interactional ratio behind two single agent compositions (be that SR＜0.5 is antagonism, 0.5≤SR≤1.5 are summation action, and SR＞1.5 are synergistic effect) according to Wadley bactericide mixture synergy evaluation assessment.
Result of the test sees the following form 6.
The joint toxicity measuring result of table 6. penconazole and tpn
Figure G2009102598502D00141
Result of the test shows: the weight ratio of penconazole and tpn is 1: 25 o'clock, and its interaction ratio is respectively 2.01, and the bactericide interaction theory according to GiSi (1985) proposes has the notable synergistic effect.
The joint toxicity measuring of test example 7 penconazoles and Wuyiencin
For the examination bacterium
Powdery mildew of cucumber (Sphaerotheca Fuligenea (Schlecht) Poll.)
For the examination cucumber variety
" the close thorn in Shandong ", cucumber is potted plant to be cultured to two open and flat phases of few leaf in the greenhouse.
The experimental concentration design
Penconazole list agent concentration of treatment is 4.8,2.4,1.2,0.6,0.3 μ g/ml, Wuyiencin list agent concentration of treatment is 6.4,3.2,1.6,0.8,0.4 μ g/ml, five concentration gradients are also established in the test of combination medicament, and other establishes the blank of not dosing, and each is handled 4 times and repeats.
Test method
With the pure water that is added with low quantity of surfactant (as Tween-80 etc.), wash and get the fresh spore that covers with on the white powder germ plant leaf blade, filter with double gauze, making spore concentration is 1 * 10 5The suspension of individual spore/mL, standby.Soup is evenly sprayed in the blade face to all wetting, treat behind the soup natural air drying standby.Use the spore suspension spray inoculation.24h inoculation before chemicals treatment.Postvaccinal examination material natural air drying moves to thermostatic chamber then, cultivates 7d～10d under the condition of 20 ℃～24 ℃ of temperature.
Investigation and method of counting
By medicine inspecting institute of the Ministry of Agriculture " agricultural chemicals indoor bioassay test rule " method, treat that the sick leaf rate of blank reaches 80% when above, the classification investigation state of an illness condition of getting a haircut everywhere, 30 leaves are investigated in every processing at least, and grade scale is: 0 grade: anosis;
1 grade: lesion area accounts for below 5% of one-piece blade area;
3 grades: lesion area accounts for 5%～15% of one-piece blade area;
5 grades: lesion area accounts for 15%～25% of one-piece blade area;
7 grades: lesion area accounts for 25%～50% of one-piece blade area;
9 grades: lesion area accounts for 50%～75% of one-piece blade area;
11 grades: lesion area accounts for more than 75% of one-piece blade area.According to survey data, calculate disease index and control efficiency that each is handled.Do regression analysis according to each drug concentration logarithm value and preventive effect probability value, calculate the EC of each medicament 50,, calculate interactional ratio behind two single agent compositions (be that SR＜0.5 is antagonism, 0.5≤SR≤1.5 are summation action, and SR＞1.5 are synergistic effect) according to Wadley bactericide mixture synergy evaluation assessment.
Result of the test sees the following form 7.
The joint toxicity measuring result of table 7. penconazole and Wuyiencin
Figure G2009102598502D00151
Figure G2009102598502D00161
Result of the test shows: the weight ratio of penconazole and Wuyiencin is 1: 1 o'clock, and its interaction ratio is respectively 2.11, and the bactericide interaction theory according to GiSi (1985) proposes has the notable synergistic effect.。
Though above the present invention is described in detail with a general description of the specific embodiments, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements all belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.

Claims (6)

1. bactericidal composition is characterized in that containing the penconazole and the arbitrary fungicide active ingredient that is selected from Fluoxastrobin, tmtd, mancozeb, zineb, Propineb, tpn and Wuyiencin of synergy effective dose.
2. according to the bactericidal composition of claim 1, it is characterized in that penconazole and the weight ratio that is selected from arbitrary fungicide active ingredient of Fluoxastrobin, tmtd, mancozeb, zineb, Propineb, tpn and Wuyiencin be preferably 20: 1～1: 40.
3. according to the bactericidal composition of claim 1-2, it is characterized in that penconazole and the weight ratio that is selected from arbitrary fungicide active ingredient of Fluoxastrobin, tmtd, mancozeb, zineb, Propineb, tpn and Wuyiencin be preferably 10: 1～1: 40.
4. according to the bactericidal composition of claim 1-3, it is characterized in that the weight ratio more preferably 3: 1～1: 10 of penconazole and Fluoxastrobin; The weight ratio of penconazole and tmtd, mancozeb, zineb, Propineb or tpn more preferably 1: 1～1: 40; The weight ratio of penconazole and Wuyiencin more preferably 5: 1～1: 5.
5. missible oil, microemulsion, aqueous emulsion, suspending agent, aqua, wetting powder, water dispersible granules or the granule formulated according to the described bactericidal composition of arbitrary claim of claim 1-4.
According to the described bactericidal composition of arbitrary claim of claim 1-4 be used to prevent and treat the powdery mildew, alternaria leaf spot of apple, ring rot of apple and the pear scab that cause by sac fungi, imperfect fungus purposes.
CN200910259850A 2009-12-16 2009-12-16 Sterilization composition containing penconazole Pending CN101779677A (en)

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Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101933514A (en) * 2010-09-03 2011-01-05 江苏七洲绿色化工股份有限公司 Bactericidal composite with triazole bactericide and chlorothalonil
CN101971839A (en) * 2010-11-21 2011-02-16 陕西美邦农药有限公司 Bactericidal composition containing cyproconazole
CN101743973B (en) * 2010-01-05 2013-03-13 陕西美邦农资贸易有限公司 Bactericidal composition containing penconazole and chlorothalonil
CN101720774B (en) * 2010-01-05 2013-03-13 陕西美邦农资贸易有限公司 Bactericide composition containing penconazole and propineb
CN101731248B (en) * 2010-01-05 2013-04-10 陕西美邦农资贸易有限公司 Penconazole-containing and thioneb-containing sterilizing composition
WO2015079334A1 (en) 2013-11-26 2015-06-04 Upl Limited A method for controlling rust
CN104705322A (en) * 2013-12-12 2015-06-17 南京华洲药业有限公司 Sterilization composition containing penconazole and ambam and use thereof
CN110839643A (en) * 2019-11-26 2020-02-28 广东药科大学 Pesticide composition for preventing and treating plant diseases and preparation method thereof

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101743973B (en) * 2010-01-05 2013-03-13 陕西美邦农资贸易有限公司 Bactericidal composition containing penconazole and chlorothalonil
CN101720774B (en) * 2010-01-05 2013-03-13 陕西美邦农资贸易有限公司 Bactericide composition containing penconazole and propineb
CN101731248B (en) * 2010-01-05 2013-04-10 陕西美邦农资贸易有限公司 Penconazole-containing and thioneb-containing sterilizing composition
CN101933514A (en) * 2010-09-03 2011-01-05 江苏七洲绿色化工股份有限公司 Bactericidal composite with triazole bactericide and chlorothalonil
CN101933514B (en) * 2010-09-03 2013-03-13 江苏七洲绿色化工股份有限公司 Bactericidal composite with triazole bactericide and chlorothalonil
CN101971839A (en) * 2010-11-21 2011-02-16 陕西美邦农药有限公司 Bactericidal composition containing cyproconazole
WO2015079334A1 (en) 2013-11-26 2015-06-04 Upl Limited A method for controlling rust
GB2532700A (en) * 2013-11-26 2016-05-25 Upl Ltd A method for controlling rust
GB2532700B (en) * 2013-11-26 2016-08-31 Upl Ltd A method for controlling rust
EP3073826A4 (en) * 2013-11-26 2016-10-19 Upl Ltd A method for controlling rust
EP3073826B1 (en) 2013-11-26 2019-10-30 UPL Limited A method for controlling soybean rust
CN104705322A (en) * 2013-12-12 2015-06-17 南京华洲药业有限公司 Sterilization composition containing penconazole and ambam and use thereof
CN110839643A (en) * 2019-11-26 2020-02-28 广东药科大学 Pesticide composition for preventing and treating plant diseases and preparation method thereof

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