CN101712992A - 一种华贵栉孔扇贝橙黄壳色分子标记及其鉴定方法和试剂盒 - Google Patents
一种华贵栉孔扇贝橙黄壳色分子标记及其鉴定方法和试剂盒 Download PDFInfo
- Publication number
- CN101712992A CN101712992A CN200910192544A CN200910192544A CN101712992A CN 101712992 A CN101712992 A CN 101712992A CN 200910192544 A CN200910192544 A CN 200910192544A CN 200910192544 A CN200910192544 A CN 200910192544A CN 101712992 A CN101712992 A CN 101712992A
- Authority
- CN
- China
- Prior art keywords
- primer
- molecular marker
- shell color
- orange
- chlamys nobilis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000003147 molecular marker Substances 0.000 title claims abstract description 31
- 238000000034 method Methods 0.000 title claims abstract description 22
- 241001526679 Mimachlamys nobilis Species 0.000 title abstract description 55
- 239000002773 nucleotide Substances 0.000 claims abstract description 22
- 125000003729 nucleotide group Chemical group 0.000 claims abstract description 22
- 230000002068 genetic effect Effects 0.000 claims abstract description 20
- 238000012408 PCR amplification Methods 0.000 claims abstract description 16
- 238000012214 genetic breeding Methods 0.000 claims abstract description 5
- 238000006243 chemical reaction Methods 0.000 claims description 14
- 241000237509 Patinopecten sp. Species 0.000 claims description 8
- 238000002360 preparation method Methods 0.000 claims description 7
- 235000020637 scallop Nutrition 0.000 claims description 4
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims description 3
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims description 3
- 241001526627 Azumapecten farreri Species 0.000 claims description 2
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 2
- 241000237505 Chlamys Species 0.000 claims 2
- 238000004458 analytical method Methods 0.000 abstract description 12
- 208000005652 acute fatty liver of pregnancy Diseases 0.000 abstract description 4
- 239000012634 fragment Substances 0.000 description 20
- 230000003321 amplification Effects 0.000 description 19
- 238000003199 nucleic acid amplification method Methods 0.000 description 19
- 238000012360 testing method Methods 0.000 description 16
- 238000001962 electrophoresis Methods 0.000 description 9
- 238000013467 fragmentation Methods 0.000 description 9
- 238000006062 fragmentation reaction Methods 0.000 description 9
- 108090000790 Enzymes Proteins 0.000 description 7
- 102000004190 Enzymes Human genes 0.000 description 7
- 239000003550 marker Substances 0.000 description 7
- 241001441955 Argopecten irradians Species 0.000 description 6
- 238000009395 breeding Methods 0.000 description 5
- 239000003086 colorant Substances 0.000 description 5
- 230000001488 breeding effect Effects 0.000 description 4
- 230000004087 circulation Effects 0.000 description 4
- 238000000605 extraction Methods 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 235000015170 shellfish Nutrition 0.000 description 4
- 238000013461 design Methods 0.000 description 3
- 239000003292 glue Substances 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 241000879903 Pteria Species 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 238000013095 identification testing Methods 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 239000011259 mixed solution Substances 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 238000012797 qualification Methods 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 241000318639 Argopecten purpuratus Species 0.000 description 1
- 102000012410 DNA Ligases Human genes 0.000 description 1
- 108010061982 DNA Ligases Proteins 0.000 description 1
- 241000237536 Mytilus edulis Species 0.000 description 1
- 241000237502 Ostreidae Species 0.000 description 1
- 241001212699 Pinctada martensii Species 0.000 description 1
- 108700005079 Recessive Genes Proteins 0.000 description 1
- 102000052708 Recessive Genes Human genes 0.000 description 1
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 238000013016 damping Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000002242 deionisation method Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 238000009399 inbreeding Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000020636 oyster Nutrition 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 1
- 239000013558 reference substance Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 238000012882 sequential analysis Methods 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 229910021642 ultra pure water Inorganic materials 0.000 description 1
- 239000012498 ultrapure water Substances 0.000 description 1
Images
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2009101925441A CN101712992B (zh) | 2009-09-22 | 2009-09-22 | 一种华贵栉孔扇贝橙黄壳色分子标记及其鉴定方法和试剂盒 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2009101925441A CN101712992B (zh) | 2009-09-22 | 2009-09-22 | 一种华贵栉孔扇贝橙黄壳色分子标记及其鉴定方法和试剂盒 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN101712992A true CN101712992A (zh) | 2010-05-26 |
CN101712992B CN101712992B (zh) | 2012-10-31 |
Family
ID=42416989
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN2009101925441A Expired - Fee Related CN101712992B (zh) | 2009-09-22 | 2009-09-22 | 一种华贵栉孔扇贝橙黄壳色分子标记及其鉴定方法和试剂盒 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN101712992B (und) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103361340A (zh) * | 2012-03-27 | 2013-10-23 | 中国科学院海洋研究所 | 海湾扇贝耐热相关热休克蛋白70基因标记及其辅助育种方法 |
CN105256056A (zh) * | 2015-11-17 | 2016-01-20 | 万超 | 一种扇贝物种特异性检测引物及应用 |
CN112695105A (zh) * | 2021-02-18 | 2021-04-23 | 辽宁省海洋水产科学研究院 | 栉孔扇贝的实时荧光pcr鉴定方法 |
CN113604584A (zh) * | 2021-08-12 | 2021-11-05 | 中国海洋大学 | 一种栉孔扇贝遗传性别相关的分子标记及其应用 |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN100420375C (zh) * | 2005-10-09 | 2008-09-24 | 中国海洋大学 | 生产具有雌核发育特征扇贝和快速建立扇贝纯系的方法 |
CN101341862B (zh) * | 2008-08-29 | 2010-12-15 | 中国科学院南海海洋研究所 | 一种橙黄壳色华贵栉孔扇贝的制种方法 |
-
2009
- 2009-09-22 CN CN2009101925441A patent/CN101712992B/zh not_active Expired - Fee Related
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103361340A (zh) * | 2012-03-27 | 2013-10-23 | 中国科学院海洋研究所 | 海湾扇贝耐热相关热休克蛋白70基因标记及其辅助育种方法 |
CN105256056A (zh) * | 2015-11-17 | 2016-01-20 | 万超 | 一种扇贝物种特异性检测引物及应用 |
CN105256056B (zh) * | 2015-11-17 | 2018-12-14 | 万超 | 一种扇贝物种特异性检测引物及应用 |
CN112695105A (zh) * | 2021-02-18 | 2021-04-23 | 辽宁省海洋水产科学研究院 | 栉孔扇贝的实时荧光pcr鉴定方法 |
CN113604584A (zh) * | 2021-08-12 | 2021-11-05 | 中国海洋大学 | 一种栉孔扇贝遗传性别相关的分子标记及其应用 |
CN113604584B (zh) * | 2021-08-12 | 2024-02-13 | 中国海洋大学 | 一种栉孔扇贝遗传性别相关的分子标记及其应用 |
Also Published As
Publication number | Publication date |
---|---|
CN101712992B (zh) | 2012-10-31 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN102174650A (zh) | 一种象耳豆根结线虫lamp快速检测方法及应用 | |
CN106011246B (zh) | 基于微流体芯片技术的5种根结线虫等温扩增检测引物序列及其应用 | |
CN116497099B (zh) | 一种苹果黄蚜钠离子通道l1014f突变位点的lamp检测引物及方法 | |
CN101712992A (zh) | 一种华贵栉孔扇贝橙黄壳色分子标记及其鉴定方法和试剂盒 | |
Dong et al. | Loop-mediated isothermal amplification assay for sensitive and rapid detection of Phytophthora capsici | |
CN107974511B (zh) | 粉棒束孢特异性检测试剂盒及其检测方法 | |
CN101586160A (zh) | 马尾松病死木中松材线虫的特异性检测引物、检测方法及其应用 | |
CN102660777A (zh) | 白叶枯病菌胁迫的普通野生稻ssh文库的构建方法 | |
CN104059909B (zh) | 马铃薯金线虫scar标记和lamp快速检测方法及应用 | |
CN101586168B (zh) | J亚群禽白血病病毒基因检测用引物组、检测方法和快速检测试剂盒 | |
CN101974633B (zh) | 一种定量检测微囊藻的方法及其专用标准品 | |
CN109868329B (zh) | 刺盘孢属特异性引物筛查检疫鉴定方法 | |
CN103667530A (zh) | 一种番茄褪绿病毒的逆转录环介导等温检测方法 | |
CN112680463B (zh) | 一种野豌豆潜隐病毒的cp基因及其应用 | |
CN106636424A (zh) | 一种原位杂交探针及其对大麦染色体组鉴定的方法 | |
CN104293775B (zh) | 抗逆杨树的分子标记、筛选方法、试剂盒及应用 | |
CN112011626B (zh) | 一种海丰沙塘鳢和河川沙塘鳢的特异性分子鉴别方法 | |
CN101086010B (zh) | 应用于赤眼蜂分子鉴定的专用引物及鉴定赤眼蜂的方法 | |
KR100625010B1 (ko) | 고추 및 파프리카에 발생하는 고추모틀바이러스,잠두위조바이러스2, 오이모자이크바이러스 및고추마일드모틀바이러스 진단용 프라이머 조합 | |
CN107586863A (zh) | 同时检测黄化病植原体、溃疡病病原菌和叶斑病病原菌的核酸、试剂盒及方法 | |
CN113699244B (zh) | 用于鉴定嗜虫书虱的lamp引物组及其应用 | |
CN110055341A (zh) | 一种识别虾夷扇贝中部着丝粒染色体的探针及其制备方法 | |
CN110578018B (zh) | 一种lamp快速检测核型多角体病毒的方法 | |
CN111471777B (zh) | 一种包含13个快速突变y-str基因座的复合扩增检测体系、方法及应用 | |
CN102041303A (zh) | 利用fAFLP标记技术分析贝类遗传多样性的方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
CF01 | Termination of patent right due to non-payment of annual fee | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20121031 Termination date: 20210922 |