CN101692837A - Pure Chinese medicine disinfectant and process for preparing same - Google Patents
Pure Chinese medicine disinfectant and process for preparing same Download PDFInfo
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Abstract
The invention discloses a pure Chinese medicine disinfectant and a process for preparing the same, which aim at providing a disinfectant with low cost, simple and convenient use and fine sterilization effect, and solve the technical problem that the existing disinfectant has strong irritation, large toxic effect and side effect and hidden using safety hazards. Selected raw medicinal materials and weight proportions thereof respectively include 20 parts of chinaberry flower, 10 parts of wrinkled gianthyssop herb, 15 parts of Chinese atractylode rhizome, 15 parts of Chinese mugwort leaf, 30 parts of tangerine peel and 30 parts of lightyellow sophora root. The process for preparing the disinfectant is selected from processes of 1, water boiling, 2, distillation, 3, reflux and 4, impregnation. The pure Chinese medicine disinfectant has strong bactericidal effect on gram-negative bacteria and gram-positive bacteria, and has the advantages of no irritation, no toxic effect or side effect, no residues, no pollution and capability of being widely applied to sterilization in environment, livestock and poultry houses and body surfaces of livestock and poultry.
Description
Technical field
The present invention relates to the disinfectant field, be specifically related to a kind ofly in Chinese herbal medicine, extract effective ingredient, be used for pure Chinese medicine antiseptic, Gram-negative bacteria and gram-positive bacteria are all had very strong killing effect animal skin sterilization and environment disinfected.
Background technology
At present, the chemosterilant of use: as the lactic acid sterilizing rate is 70%, but the stimulation nature and flavor are arranged, and corrosivity is strong, and respiratory tract is had stimulation, and furniture, electrical equipment, metal are had infringement; Peracetic acid all has efficient, quick-acting broad-spectrum disinfectant effects to bacterial reproduction brood cell, fungi, virus etc., it is desirable air sanitizer, but mucous membrane there is excitant, genus is corrosive, though fumigation was to the reduction of human body skin mucous membrane respiratory tract after the equivalent dilution reduced concentration, sterilizing rate is below 80%; Formaldehyde is the aldehydes high effect disinfectants, and the steam that produces when stifling has strong impulse to eyes and respiratory tract, reach Disinfection Effect and also need fumigate the airtight 10h in back, and because carcinogenesis is arranged, be tending towards superseded.Because chemical disinfection has stronger stimulation and damage to human body, therefore, people urgently wish from natural Chinese medicinal herb, to develop Disinfection Effect good, have no adverse reaction, Chinese medicine air sanitizer easy to use.
Chinese medicine antiseptic because it is safe in utilization, satisfactory for result, fragrant odour, harmless, article are not had advantage such as corrosiveness, its most outstanding characteristics are noresidues, free from environmental pollution, are quite paid close attention in recent years.
China Patent No. is that 2007100394446 patent of invention discloses Chinese medicine air sanitizer of a kind of anti-avian influenza and preparation method thereof; The component of this pure Chinese medicine anti-avian influenza air disinfectant and weight ratio are 1~10 part of hypericum perforatum, 1~10 part of Huang Ling, 1~10 part of isatis root, 1~10 part of honeysuckle, 1~10 part of wrinkled giant hyssop, 1~10 part of eupatorium.The preparation method of this disinfectant: above-mentioned medicinal material is mixed the back water decoct 1-3 time, boiled 2-3 hour at every turn, all collect volatile oil and decocting liquid at every turn; Collecting decoction filters, and leaves standstill 24 as a child, draws supernatant, filters, and concentrates, and alcohol precipitation reclaims ethanol; Add entry dilution, stir evenly, leave standstill, get supernatant, filter, add the volatile oil of being collected, and soup transferred to contain crude drug 4-10%, stir evenly,
Packing has been owing to adopted above-mentioned technical solution, disinfectant nonirritant of the present invention, and no toxicity can effectively be killed various pathogenic microorganisms, can effectively resist the invasion and attack of avian influenza virus especially, prevents and treats various diseases such as bird flu.
China Patent No. is that 2006100153801 patent of invention discloses a kind of Chinese herbal disinfectant.Technology of the present invention is as follows: contain the 0.5-1.5kg rheum officinale in 60% alcohol of every 3-7L; In proportion 60% alcohol and rheum officinale are placed percolation filter, flooded 20~30 hours, the usage filter gets final product.If will being diluted to 60% alcohol, uses 95% industrial alcohol.
Summary of the invention
The object of the present invention is to provide a kind of with low cost, easy to use, disinfectant that sterilization effect is good.Problem to be solved is: it is strong to overcome existing disinfectant excitant, the big and technical barrier that has hidden danger safe in utilization of toxic and side effect.
The present invention has adopted following technical scheme for achieving the above object:
Raw medicinal material and composition by weight thereof that the present invention chooses are respectively: 20 parts of China trees, 10 parts of wrinkled giant hyssops, 15 parts of rhizoma atractylodis, 15 parts of tarragons, 30 parts of dried orange peels, 30 parts of kuh-sengs; It is the liquid disinfectant that optional decocting method, the way of distillation, circumfluence method, infusion process are made.
Its preparation method division is as follows:
One, decocting method: 40 parts of the materials of getting it filled, add the water of 480 weight portions, soak to heat after half an hour and boiled 40 minutes, make active ingredient fully fried; Separate decoction liquor, the dregs of a decoction are fried in shallow oil 2 times again; Merge three times decoction liquor; Make the decoction liquor of 400 weight portions altogether; Add ethanol then and make the alcohol amount of containing reach 60% and remove impurity such as desizing, filter, get supernatant, reclaim ethanol with Rotary Evaporators to get 130 weight portion extracts; Add 10 weight portion stabilizing agent Tween-80s, originally the Hun Zhuo liquid clear that becomes filters; Packing refrigeration is standby;
Two, the way of distillation: 40 parts of the materials of getting it filled, water 480 weight portions are collected cut in the medium and small fire distillation of distillation equipment 1 hour; The dregs of a decoction filter collects filtrate; In filtrate, add precipitation with alcohol impurity, remove ethanol, get 200 weight portion extracts with rotary evaporation; Merge extract and cut; Packing refrigeration is standby;
Three, circumfluence method: 40 parts of the materials of getting it filled, be bundled into cylinder with filter paper and pack in the cable type extractor according, in flask, add the ethanol of 480 weight portions, added hot reflux 0.5 hour; Cooling is filtered, and collects filtrate, adds ethanol in filtrate, sedimentation and filtration, and rotary evaporation reclaims ethanol and removes impurity, obtains proposing liquid 320 weight portions, adds the Tween-80 stabilizing agent, and packing refrigeration is standby;
Four, infusion process: get the dipping vessel that 40 parts of medicinal materials place 500 weight portions, the water that adds 480 weight portions soaked two days as extraction solvent, and active ingredient is fully leached.Filter, filtrate 320 weight portions, add ethanol and make it contain the alcohol amount to reach 60%, behind the sedimentation and filtration, the supernatant rotary evaporation is removed impurity reclaim ethanol, make soup 200 weight portions, add the stabilizing agent Tween 80, packing refrigeration is standby.
In the raw medicinal material that the present invention chooses, China tree: heat-clearing and damp-drying drug, killing parasites to relieve itshing.Wrinkled giant hyssop: dampness elimination, relieving summer-heat, deliver, preventing or arresting vomiting; Antibiotic, anti-conveyor screw and antivirus action are arranged.Rhizoma atractylodis: drying damp and strengthening spleen, wind-damp dispelling, sweating makes eye bright; This product is controlled the key medicine of damp retention in middle-jiao.Tarragon: hot, bitter, warm; Warming channel for arresting bleeding, eliminating cold to stop pain; This product has the merit of dampness removing to stop itchin.Kuh-seng: heat-clearing and damp-drying drug, killing parasites to relieve itshing, diuresis.Dried orange peel: the bitter substance of dried orange peel is representative " limonoid " with lemon glycosides and bitter principle, and this limonoid flavor is gentle, easily is dissolved in water, and its bitter taste and other tastes are in harmonious proportion mutually, can form original in style local flavor.Dried orange peel contains compositions such as volatile oil, hesperidine, Cobastab, C, and is shared with tarragon, wrinkled giant hyssop, has the effect of strong repelling and killing bugs.China tree, tarragon, wrinkled giant hyssop, kuh-seng are the key medicine of sterilization and disinfection, and all medicines are shared, strengthen the sterilization and disinfection effect.
Following for utilizing the present invention specifically to test
1, bacteriostatic experiment
Between sterile working, dip in and get bacterial classification, be uniformly coated on the culture medium flat plate with oese.Aseptic metal card punch with diameter 5mm is evenly made a call to 3 holes on each medium, in the various disinfectants difference inlet holes that prepare.In contrast, prepare an aseptic culture medium that does not drip medicine, be placed on together and cultivate 24~36h in 37 ℃ of incubators.Each disinfectant of observed and recorded is to staphylococcus, colibacillary antibacterial circle diameter.
2, local irritation test
Rabbit eye drip method: 4 of rabbit, divide 2 groups, the soup that the way of distillation and decocting method are made is with 100 times of dilutions of physiological saline respectively, and 1 group of distillate with dilution drips in the conjunctival sac left, and right eye is done contrast; 2 groups of decocting liquid with dilution, the method for dripping is the same.Observing 1 left eye in per 10 minutes changes.
Wipe 4 of ear Legalists rabbits, divide 2 groups, the hard of hearing portion in a soup left side that makes with the way of distillation and decocting method observed 1 left ear skin and changes in per 10 minutes respectively.
3, pig house, the test of hen house spraying disinfection
The soup that the way of distillation and decocting method are made with 200 times of dilutions of ordinary water after, medicine liquid spray in pig (chicken) house, and is sprayed pig (chicken) body surface, 1 time weekly, in 3 weeks of logotype, observe pig (chicken) reaction and air cleaning situation.
Result and analysis
Table 1 Escherichia coli experimental result (antibacterial circle diameter mm)
Table 2 staphylococcus experimental result (antibacterial circle diameter mm)
Above result shows: to Escherichia coli, the way of distillation and water-boiling method do not have significant difference, and other scheme drug effect differences are all extremely remarkable.Illustrate that this Chinese medicine preparation process is relatively stricter to the working condition requirement, select production technology, control working condition could guarantee product quality.
The local irritation result of the test
Rabbit eye drip method: behind the rabbit eye drip within 10 minutes left eye a small amount of tear is arranged, promptly recover after 10 minutes normal.Illustrate that these two kinds of Chinese medicine antiseptics have slight excitant to mucous membrane, but eyesight is not had influence.
Wiping ear test: with system stoste to wipe behind the ear in 20 minutes ear skin slightly gradually red, the thin blood vessel of left tragus is thicker slightly than auris dextra, but does not see to shake the head and grab the ear phenomenon.Illustrate that Chinese medicine antiseptic has slight expansion epidermis capillary action, but excitant is not strong.
Pig house, the test of hen house spraying disinfection
According to 2 pig houses, 2 hen house evidences: spraying back 1 all Nei Shenei mosquitos and flies obviously reduce, and pig, chicken have no adverse reaction.Illustrate that Chinese medicine antiseptic has good repelling and killing bugs effect, can reduce summer like this by the infectious disease of hematophagus propagation and the incidence of disease of hematophagia parasitosis.
Conclusion: by above test as can be known, pure Chinese medicine antiseptic 1. of the present invention all has very strong bactericidal action to Gram-negative bacteria and gram-positive bacteria, and is wherein best with way of distillation effect.2. Chinese medicine antiseptic of the present invention has the repelling and killing bugs effect, and livestock and poultry are given up interior air, the elimination communication media is significant for purifying for this.3. Chinese medicine antiseptic nonirritant of the present invention has no side effect, and noresidue is pollution-free, can be widely used in environment, livestock and poultry house and the sterilization of livestock and poultry body surface.
Embodiment
Raw medicinal material and composition by weight thereof that pure Chinese medicine antiseptic of the present invention is chosen are respectively: hardship
Embodiment
Raw medicinal material and composition by weight thereof that pure Chinese medicine antiseptic of the present invention is chosen are respectively: 20 parts of China trees, 10 parts of wrinkled giant hyssops, 15 parts of rhizoma atractylodis, 15 parts of tarragons, 30 parts of dried orange peels, 30 parts of kuh-sengs; It is the liquid disinfectant that optional decocting method, the way of distillation, circumfluence method, infusion process are made.
Its preparation method division is as follows:
One, decocting method: 40 parts of the materials of getting it filled, the water logging bubble that adds 480 weight portions heat after half an hour and boiled 40 minutes, make active ingredient fully fried; Separate decoction liquor, the dregs of a decoction are fried in shallow oil 2 times again; Merge three times decoction liquor; Make the decoction liquor of 400 weight portions altogether; Add ethanol then and make the alcohol amount of containing reach 60% and remove impurity such as desizing, filter, get supernatant, reclaim ethanol with Rotary Evaporators to get 130 weight portion extracts; Add 10 weight portion stabilizing agent Tween-80s, originally the Hun Zhuo liquid clear that becomes filters; Packing refrigeration is standby;
Two, the way of distillation: 40 parts of the materials of getting it filled, water 480 weight portions are collected cut in the medium and small fire distillation of distillation equipment 1 hour; The dregs of a decoction filter collects filtrate; In filtrate, add precipitation with alcohol impurity, remove ethanol, get 200 weight portion extracts with rotary evaporation; Merge extract and cut; Packing refrigeration is standby;
Three, circumfluence method: 40 parts of the materials of getting it filled, be bundled into cylinder with filter paper and pack in the cable type extractor according, in flask, add the ethanol of 480 weight portions, added hot reflux 0.5 hour; Cooling is filtered, and collects filtrate, adds ethanol in filtrate, sedimentation and filtration, and rotary evaporation reclaims ethanol and removes impurity, obtains proposing liquid 320 weight portions, adds the Tween-80 stabilizing agent, and packing refrigeration is standby;
Four, infusion process: get the dipping vessel that 40 parts of medicinal materials place 500 weight portions, the water that adds 480 weight portions soaked two days as extraction solvent, and active ingredient is fully leached.Filter, filtrate 320 weight portions, add ethanol and make it contain the alcohol amount to reach 60%, behind the sedimentation and filtration, the supernatant rotary evaporation is removed impurity reclaim ethanol, make soup 200 weight portions, add the stabilizing agent Tween-80, packing refrigeration is standby.
Claims (2)
1. pure Chinese medicine antiseptic and preparation method thereof, it is characterized in that: raw medicinal material and the composition by weight thereof chosen are respectively: 20 parts of China trees, 10 parts of wrinkled giant hyssops, 15 parts of rhizoma atractylodis, 15 parts of tarragons, 30 parts of dried orange peels, 30 parts of kuh-sengs; It is the liquid disinfectant that optional decocting method, the way of distillation, circumfluence method, infusion process are made.
2. according to described a kind of pure Chinese medicine antiseptic of claim 1 and preparation method thereof, it is characterized in that: the preparation method of described disinfectant is following four kinds:
One, decocting method: 40 parts of the materials of getting it filled, the water logging bubble that adds 480 weight portions heat after half an hour and boiled 40 minutes, make active ingredient fully fried; Separate decoction liquor, the dregs of a decoction are fried in shallow oil 2 times again; Merge three times decoction liquor; Make the decoction liquor of 400 weight portions altogether; Add ethanol then and make the alcohol amount of containing reach 60% and remove impurity such as desizing, filter, get supernatant, reclaim ethanol with Rotary Evaporators to get 130 weight portion extracts; Add 10 weight portion stabilizing agent Tween-80s, originally the Hun Zhuo liquid clear that becomes filters; Packing refrigeration is standby;
Two, the way of distillation: 40 parts of the materials of getting it filled, water 480 weight portions are collected cut in the medium and small fire distillation of distillation equipment 1 hour; The dregs of a decoction filter collects filtrate; In filtrate, add precipitation with alcohol impurity, remove ethanol, get 200 weight portion extracts with rotary evaporation; Merge extract and cut; Packing refrigeration is standby;
Three, circumfluence method: 40 parts of the materials of getting it filled, be bundled into cylinder with filter paper and pack in the cable type extractor according, in flask, add the ethanol of 480 weight portions, added hot reflux 0.5 hour; Cooling is filtered, and collects filtrate, adds ethanol in filtrate, sedimentation and filtration, and rotary evaporation reclaims ethanol and removes impurity, obtains proposing liquid 320 weight portions, adds the Tween-80 stabilizing agent, and packing refrigeration is standby;
Four, infusion process: get the dipping vessel that 40 parts of medicinal materials place 500 weight portions, the water that adds 480 weight portions soaked two days as extraction solvent, and active ingredient is fully leached.Filter, filtrate 320 weight portions, add ethanol and make it contain the alcohol amount to reach 60%, behind the sedimentation and filtration, the supernatant rotary evaporation is removed impurity reclaim ethanol, make soup 200 weight portions, add the stabilizing agent Tween-80, packing refrigeration is standby.
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CN101731292B (en) * | 2009-11-23 | 2013-03-27 | 李远林 | Production method of biopesticide |
CN103039544A (en) * | 2012-12-31 | 2013-04-17 | 周静 | Agent for disinfection of operating room |
CN104026173A (en) * | 2014-06-24 | 2014-09-10 | 杨美田 | Disinfectant special for inspection instrument in inspection department and preparation method of disinfectant |
CN104719356A (en) * | 2013-12-20 | 2015-06-24 | 刘艳红 | Disinfectant solution and preparation method thereof |
CN105010424A (en) * | 2015-07-13 | 2015-11-04 | 江苏神涛环保科技有限公司 | Disinfectant composition |
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CN104026173B (en) * | 2014-06-24 | 2016-05-11 | 李淑娟 | A kind of clinical laboratory inspection apparatus appropriative disinfector and preparation method thereof |
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CN105010424A (en) * | 2015-07-13 | 2015-11-04 | 江苏神涛环保科技有限公司 | Disinfectant composition |
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Application publication date: 20100414 |