CN112167270A - Agastache rugosus extract and disinfectant containing same - Google Patents

Abstract

The invention relates to a wrinkled giant hyssop extract, in particular to a wrinkled giant hyssop extract and a disinfectant containing the wrinkled giant hyssop extract. The invention provides a preparation method of agastache rugosus extract, which comprises the following steps: s1, weighing agastache rugosus, cleaning to remove impurities, and crushing into coarse powder; s2, adding 6-8 times of distilled water into the coarse powder obtained in the step S1, soaking, and performing reflux extraction; s3, adding 2-5 times of distilled water into the extract obtained in the step S2, and then performing microwave extraction; s4, centrifuging the extract obtained in the step S3 to obtain supernatant, namely the agastache rugosus extract. The disinfectant containing the agastache rugosus extract provided by the invention has a good inhibition effect on gram-positive bacteria and gram-negative bacteria.

Description

Agastache rugosus extract and disinfectant containing same

Technical Field

The invention relates to a wrinkled giant hyssop extract, in particular to a wrinkled giant hyssop extract and a disinfectant containing the wrinkled giant hyssop extract.

Background

Agastache rugosus, academic name: agastache rugosa; also named: hexiang, cang gang, shan fennel and so on, which are perennial herb plants of Labiatae, Labiatae and Agastache and are homologous plant of medicine and food published by Ministry of health of China. Due to various application values, agastache rugosus has been a hot point of research of people in recent years, and a large number of researches prove that agastache rugosus and extracts thereof have good nutritional value, medicinal value and economic value. For example, the antioxidant action of Agastache rugosa, the bacteriostatic and antibacterial action of Agastache rugosa extract.

The agastache rugosus has a great application prospect as a medicine-food homologous plant, the current research on the agastache rugosus mainly focuses on the aspects of chemical component analysis research, volatile oil determination and the like, and the research on the antibacterial and antibacterial aspects of the agastache rugosus extract is less. The antibacterial effect of the water extract and alcohol extract of the ageratum is reported in the prior art (preliminary study on antibacterial effect of the ageratum), and the result shows that the antibacterial effect of the alcohol extract of the ageratum is obviously higher than that of the water extract. On one hand, 70% ethanol is used in the ethanol extract, the ethanol is a flammable and volatile colorless transparent liquid at normal temperature and normal pressure, the ethanol can be mutually dissolved with water in any proportion, the formed alcohol solution is flammable and volatile, and the vapor of the ethanol can form an explosive mixture with air; the 70% ethanol is flammable and belongs to dangerous chemicals, and is easy to cause casualties and property loss in the transportation, loading, unloading and storage processes. On the other hand, the water extract and alcohol extract of herba Agastaches are both prepared by ultrasonic extraction method, herba Agastaches is limited by ultrasonic attenuation factor, the effective ultrasonic region is annular, and if the diameter of the extraction tank is too large, ultrasonic blank area will be formed; and the ultrasonic equipment has higher manufacturing difficulty and the cost cannot be accepted by users.

Disclosure of Invention

The invention aims to overcome the defects of the background technology and provide a safe, simple and low-cost preparation method of agastache rugosus extract.

The purpose of the invention is realized by the following technical scheme:

in a first aspect, the present invention provides a method for preparing an extract of Agastache rugosa, said method comprising the steps of:

s1, weighing agastache rugosus, cleaning to remove impurities, and crushing into coarse powder;

s2, adding 6-8 times of distilled water into the coarse powder obtained in the step S1, soaking, and performing reflux extraction;

s3, adding 2-5 times of distilled water into the extract obtained in the step S2, and then performing microwave extraction;

s4, centrifuging the extract obtained in the step S3 to obtain supernatant, namely the agastache rugosus extract.

In a preferred embodiment, the soaking time of the distilled water in step S2 is 20-30min, and the extraction time of the reflux extraction in step S2 is 50-60 min.

In a preferred embodiment, the microwave power extracted by the microwave extraction in step S3 is 500W, and the time is 30 min.

In a second aspect, a Agastache rugosa extract is provided, which is prepared by the preparation method of the present invention.

In a third aspect, there is provided a disinfectant comprising the extract of Agastache rugosa of the present invention.

In a preferred embodiment, the disinfectant further comprises honeysuckle extract, dandelion extract, apple peel extract, houttuynia cordata extract, dried orange peel extract and an auxiliary agent, wherein the auxiliary agent is prepared from corn starch, polypropylene glycol and carboxymethyl chitosan.

In a preferred embodiment, the weight portion of the corn starch is 3-8, the weight portion of the polypropylene glycol is 5-10, and the weight portion of the carboxymethyl chitosan is 3-5.

In a preferred embodiment, the honeysuckle extract is 5-15 parts by weight, the dandelion extract is 12-20 parts by weight, the apple peel extract is 13-18 parts by weight, the houttuynia cordata extract is 10-20 parts by weight, the dried orange peel extract is 8-12 parts by weight, and the agastache rugosus extract is 6-15 parts by weight.

In a fourth aspect, a method for preparing a disinfectant according to the present invention is provided, the method comprising the steps of:

s1, preparing a honeysuckle extract, a dandelion extract, an apple peel extract, a houttuynia cordata extract and a tangerine peel extract, mixing the prepared extracts, and dividing the mixed extracts into two parts, wherein one part is a mixed extract A, and the other part is a mixed extract B;

s2, preparing a wrinkled gianthyssop herb extract, and equally dividing the wrinkled gianthyssop herb extract into two parts, wherein one part is a wrinkled gianthyssop herb extract A, and the other part is a wrinkled gianthyssop herb extract B;

s3, preparing an auxiliary agent, namely dividing the auxiliary agent into two parts, wherein one part is an auxiliary agent A, and the other part is an auxiliary agent B;

s4, mixing the mixed extract A and the auxiliary A, slowly adding 60% ethanol at 50-60 ℃, stirring while adding, cooling to room temperature, and adding the agastache rugosus extract A;

s5, mixing the mixed extract B and the auxiliary agent B, slowly adding 70% ethanol at the temperature of 60-70 ℃, stirring while adding, cooling to room temperature, and adding the agastache rugosus extract B;

and S6, combining the mixture obtained in the step S4 and the mixture obtained in the step S5, and then purifying to obtain the disinfectant.

In a fifth aspect, the invention provides the use of the disinfectant in disinfection, sterilization and disease control.

Compared with the prior art, the invention has the beneficial effects that:

the preparation method of the agastache rugosus extract provided by the invention avoids the danger of using inflammable materials.

The preparation method of the agastache rugosus extract has the advantages of short extraction time, simple equipment and less investment by using a microwave extraction method.

According to the preparation method of the agastache rugosus extract, provided by the invention, reflux extraction and microwave extraction are combined, and water extraction is matched, so that the cost is reduced, and meanwhile, the antibacterial and bacteriostatic effects of the agastache rugosus extract are ensured.

The disinfectant containing the agastache rugosus extract provided by the invention can better exert antibacterial and bacteriostatic effects by being matched with the honeysuckle extract, the dandelion extract, the apple peel extract, the houttuynia cordata extract and the tangerine peel extract; meanwhile, the auxiliary agent is matched, so that the antibacterial and bacteriostatic effects of the disinfectant are maximized.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention. It is to be understood that the following description is only illustrative of the present invention and is not to be construed as limiting the present invention.

The terms "comprises," "comprising," "includes," "including," "has," "having," "contains," "containing," or any other variation thereof, as used herein, are intended to cover a non-exclusive inclusion. For example, a composition, process, method, article, or apparatus that comprises a list of elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, process, method, article, or apparatus.

The conjunction "consisting of …" excludes any unspecified elements, steps or components. If used in a claim, the phrase is intended to claim as closed, meaning that it does not contain materials other than those described, except for the conventional impurities associated therewith. When the phrase "consisting of …" appears in a clause of the subject matter of the claims rather than immediately after the subject matter, it defines only the elements described in the clause; other elements are not excluded from the claims as a whole.

When an amount, concentration, or other value or parameter is expressed as a range, preferred range, or as a range of upper preferable values and lower preferable values, this is to be understood as specifically disclosing all ranges formed from any pair of any upper range limit or preferred value and any lower range limit or preferred value, regardless of whether ranges are separately disclosed. For example, when a range of "1 to 5" is disclosed, the described range should be interpreted to include the ranges "1 to 4", "1 to 3", "1 to 2 and 4 to 5", "1 to 3 and 5", and the like. When a range of values is described herein, unless otherwise stated, the range is intended to include the endpoints thereof and all integers and fractions within the range.

Approximating language, as used herein throughout the specification and claims, is intended to modify a quantity, such that the invention is not limited to the specific quantity, but includes portions that are literally received for modification without substantial change in the basic function to which the invention is related. Accordingly, the use of "about" to modify a numerical value means that the invention is not limited to the precise value. In some instances, the approximating language may correspond to the precision of an instrument for measuring the value. In the present description and claims, range limitations may be combined and/or interchanged, including all sub-ranges contained therein if not otherwise stated.

The indefinite articles "a" and "an" preceding an element or component of the invention are not intended to limit the number requirement (i.e., the number of occurrences) of the element or component. Thus, "a" or "an" should be read to include one or at least one, and the singular form of an element or component also includes the plural unless the number clearly indicates the singular.

Example 1

This example provides an extract of Agastache rugosa, prepared by a method comprising the steps of:

s1, weighing agastache rugosus, cleaning to remove impurities, and crushing into coarse powder;

s2, adding 6 times of distilled water into the coarse powder obtained in the step S1, soaking for 20min, and performing reflux extraction for 50 min;

s3, adding 5 times of distilled water into the extract obtained in the step S2, and performing 500W microwave extraction for 30 min;

s4, centrifuging the extract obtained in the step S3 to obtain supernatant, namely the agastache rugosus extract.

Example 2

The embodiment provides a disinfectant, which comprises 5 parts by weight of honeysuckle extract, 12 parts by weight of dandelion extract, 13 parts by weight of apple peel extract, 10 parts by weight of houttuynia cordata extract, 8 parts by weight of dried orange peel extract, 6 parts by weight of agastache rugosus extract and an auxiliary agent, wherein the auxiliary agent is prepared from 3 parts by weight of corn starch, 5 parts by weight of polypropylene glycol and 3 parts by weight of carboxymethyl chitosan;

the preparation method of the agastache rugosus extract comprises the following steps of:

s1, weighing agastache rugosus, cleaning to remove impurities, and crushing into coarse powder;

s2, adding 6 times of distilled water into the coarse powder obtained in the step S1, soaking for 20min, and performing reflux extraction for 50 min;

s3, adding 5 times of distilled water into the extract obtained in the step S2, and performing 500W microwave extraction for 30 min;

s4, centrifuging the extract obtained in the step S3 to obtain supernatant, namely the agastache rugosus extract;

the preparation method of the disinfectant comprises the following steps:

s1, preparing a honeysuckle extract, a dandelion extract, an apple peel extract, a houttuynia cordata extract and a tangerine peel extract, mixing the prepared extracts, and dividing the mixed extracts into two parts, wherein one part is a mixed extract A, and the other part is a mixed extract B;

s2, preparing a wrinkled gianthyssop herb extract, and equally dividing the wrinkled gianthyssop herb extract into two parts, wherein one part is a wrinkled gianthyssop herb extract A, and the other part is a wrinkled gianthyssop herb extract B;

s3, preparing an auxiliary agent, namely dividing the auxiliary agent into two parts, wherein one part is an auxiliary agent A, and the other part is an auxiliary agent B;

s4, mixing the mixed extract A and the auxiliary agent A, slowly adding 60% ethanol at 50 ℃, stirring while adding, cooling to room temperature, and adding the agastache rugosus extract A;

s5, mixing the mixed extract B and the auxiliary agent B, slowly adding 70% ethanol at the temperature of 60 ℃, stirring while adding, and adding the agastache rugosus extract B after the temperature is reduced to room temperature;

and S6, combining the mixture obtained in the step S4 and the mixture obtained in the step S5, and then purifying to obtain the disinfectant.

Example 3

The embodiment provides a disinfectant, which comprises 15 parts by weight of honeysuckle extract, 20 parts by weight of dandelion extract, 18 parts by weight of apple peel extract, 20 parts by weight of houttuynia cordata extract, 12 parts by weight of dried orange peel extract, 15 parts by weight of agastache rugosus extract and an auxiliary agent, wherein the auxiliary agent is prepared from 8 parts by weight of corn starch, 10 parts by weight of polypropylene glycol and 5 parts by weight of carboxymethyl chitosan;

the preparation method of the agastache rugosus extract comprises the following steps of:

s1, weighing agastache rugosus, cleaning to remove impurities, and crushing into coarse powder;

s2, adding 6 times of distilled water into the coarse powder obtained in the step S1, soaking for 20min, and performing reflux extraction for 50 min;

s3, adding 5 times of distilled water into the extract obtained in the step S2, and performing 500W microwave extraction for 30 min;

s4, centrifuging the extract obtained in the step S3 to obtain supernatant, namely the agastache rugosus extract;

the preparation method of the disinfectant comprises the following steps:

s1, preparing a honeysuckle extract, a dandelion extract, an apple peel extract, a houttuynia cordata extract and a tangerine peel extract, mixing the prepared extracts, and dividing the mixed extracts into two parts, wherein one part is a mixed extract A, and the other part is a mixed extract B;

s2, preparing a wrinkled gianthyssop herb extract, and equally dividing the wrinkled gianthyssop herb extract into two parts, wherein one part is a wrinkled gianthyssop herb extract A, and the other part is a wrinkled gianthyssop herb extract B;

s3, preparing an auxiliary agent, namely dividing the auxiliary agent into two parts, wherein one part is an auxiliary agent A, and the other part is an auxiliary agent B;

s4, mixing the mixed extract A and the auxiliary agent A, slowly adding 60% ethanol at 50 ℃, stirring while adding, cooling to room temperature, and adding the agastache rugosus extract A;

s5, mixing the mixed extract B and the auxiliary agent B, slowly adding 70% ethanol at the temperature of 60 ℃, stirring while adding, and adding the agastache rugosus extract B after the temperature is reduced to room temperature;

and S6, combining the mixture obtained in the step S4 and the mixture obtained in the step S5, and then purifying to obtain the disinfectant.

Example 4

The embodiment provides a disinfectant, which comprises 10 parts by weight of honeysuckle extract, 15 parts by weight of dandelion extract, 16 parts by weight of apple peel extract, 12 parts by weight of houttuynia cordata extract, 11 parts by weight of dried orange peel extract, 8 parts by weight of agastache rugosus extract and an auxiliary agent, wherein the auxiliary agent is prepared from 6 parts by weight of corn starch, 6 parts by weight of polypropylene glycol and 4 parts by weight of carboxymethyl chitosan;

the preparation method of the agastache rugosus extract comprises the following steps of:

s1, weighing agastache rugosus, cleaning to remove impurities, and crushing into coarse powder;

s2, adding 6 times of distilled water into the coarse powder obtained in the step S1, soaking for 20min, and performing reflux extraction for 50 min;

s3, adding 5 times of distilled water into the extract obtained in the step S2, and performing 500W microwave extraction for 30 min;

s4, centrifuging the extract obtained in the step S3 to obtain supernatant, namely the agastache rugosus extract;

the preparation method of the disinfectant comprises the following steps:

s1, preparing a honeysuckle extract, a dandelion extract, an apple peel extract, a houttuynia cordata extract and a tangerine peel extract, mixing the prepared extracts, and dividing the mixed extracts into two parts, wherein one part is a mixed extract A, and the other part is a mixed extract B;

s2, preparing a wrinkled gianthyssop herb extract, and equally dividing the wrinkled gianthyssop herb extract into two parts, wherein one part is a wrinkled gianthyssop herb extract A, and the other part is a wrinkled gianthyssop herb extract B;

s3, preparing an auxiliary agent, namely dividing the auxiliary agent into two parts, wherein one part is an auxiliary agent A, and the other part is an auxiliary agent B;

s4, mixing the mixed extract A and the auxiliary agent A, slowly adding 60% ethanol at 50 ℃, stirring while adding, cooling to room temperature, and adding the agastache rugosus extract A;

s5, mixing the mixed extract B and the auxiliary agent B, slowly adding 70% ethanol at the temperature of 60 ℃, stirring while adding, and adding the agastache rugosus extract B after the temperature is reduced to room temperature;

and S6, combining the mixture obtained in the step S4 and the mixture obtained in the step S5, and then purifying to obtain the disinfectant.

Comparative example 1

This comparative example provides an extract of Agastache rugosa prepared by a method comprising the steps of:

s1, weighing agastache rugosus, cleaning to remove impurities, and crushing into coarse powder;

s2, adding 70% ethanol in an amount which is 6 times that of the coarse powder obtained in the step S1, soaking for 20min, and then carrying out reflux extraction for 50 min;

s3, adding 5 times of 70% ethanol into the extract obtained in the step S2, and performing 500W microwave extraction for 30 min;

s4, centrifuging the extract obtained in the step S3 to obtain supernatant, namely the agastache rugosus extract.

Comparative example 2

The comparative example provides a disinfectant comprising 15 parts by weight of a dandelion extract, 16 parts by weight of an apple peel extract, 12 parts by weight of a houttuynia cordata extract, 11 parts by weight of a tangerine peel extract, 8 parts by weight of a agastache rugosus extract and an auxiliary agent, wherein the auxiliary agent is prepared from 6 parts by weight of corn starch, 6 parts by weight of polypropylene glycol and 4 parts by weight of carboxymethyl chitosan;

the preparation method of the agastache rugosus extract comprises the following steps of:

s1, weighing agastache rugosus, cleaning to remove impurities, and crushing into coarse powder;

s2, adding 6 times of distilled water into the coarse powder obtained in the step S1, soaking for 20min, and performing reflux extraction for 50 min;

s3, adding 5 times of distilled water into the extract obtained in the step S2, and performing 500W microwave extraction for 30 min;

s4, centrifuging the extract obtained in the step S3 to obtain supernatant, namely the agastache rugosus extract;

the preparation method of the comparative example disinfectant comprises the following steps:

s1, preparing a dandelion extract, an apple peel extract, a houttuynia cordata extract and a dried orange peel extract, mixing the prepared extracts, and dividing the mixed extracts into two parts, wherein one part is a mixed extract A, and the other part is a mixed extract B;

s2, preparing a wrinkled gianthyssop herb extract, and equally dividing the wrinkled gianthyssop herb extract into two parts, wherein one part is a wrinkled gianthyssop herb extract A, and the other part is a wrinkled gianthyssop herb extract B;

s3, preparing an auxiliary agent, namely dividing the auxiliary agent into two parts, wherein one part is an auxiliary agent A, and the other part is an auxiliary agent B;

s4, mixing the mixed extract A and the auxiliary agent A, slowly adding 60% ethanol at 50 ℃, stirring while adding, cooling to room temperature, and adding the agastache rugosus extract A;

s5, mixing the mixed extract B and the auxiliary agent B, slowly adding 70% ethanol at the temperature of 60 ℃, stirring while adding, and adding the agastache rugosus extract B after the temperature is reduced to room temperature;

and S6, combining the mixture obtained in the step S4 and the mixture obtained in the step S5, and then purifying to obtain the disinfectant.

Comparative example 3

The comparative example provides a disinfectant comprising 10 parts by weight of a honeysuckle extract, 15 parts by weight of a dandelion extract, 16 parts by weight of an apple peel extract, 12 parts by weight of a houttuynia cordata extract, 11 parts by weight of a tangerine peel extract, 8 parts by weight of a wrinkled gianthyssop herb extract and an auxiliary agent, wherein the auxiliary agent is prepared from 6 parts by weight of polypropylene glycol and 4 parts by weight of carboxymethyl chitosan;

the preparation method of the agastache rugosus extract comprises the following steps of:

s1, weighing agastache rugosus, cleaning to remove impurities, and crushing into coarse powder;

s2, adding 6 times of distilled water into the coarse powder obtained in the step S1, soaking for 20min, and performing reflux extraction for 50 min;

s3, adding 5 times of distilled water into the extract obtained in the step S2, and performing 500W microwave extraction for 30 min;

s4, centrifuging the extract obtained in the step S3 to obtain supernatant, namely the agastache rugosus extract;

the preparation method of the comparative example disinfectant comprises the following steps:

s1, preparing a honeysuckle extract, a dandelion extract, an apple peel extract, a houttuynia cordata extract and a tangerine peel extract, mixing the prepared extracts, and dividing the mixed extracts into two parts, wherein one part is a mixed extract A, and the other part is a mixed extract B;

s2, preparing a wrinkled gianthyssop herb extract, and equally dividing the wrinkled gianthyssop herb extract into two parts, wherein one part is a wrinkled gianthyssop herb extract A, and the other part is a wrinkled gianthyssop herb extract B;

s3, preparing an auxiliary agent, namely dividing the auxiliary agent into two parts, wherein one part is an auxiliary agent A, and the other part is an auxiliary agent B;

s4, mixing the mixed extract A and the auxiliary agent A, slowly adding 60% ethanol at 50 ℃, stirring while adding, cooling to room temperature, and adding the agastache rugosus extract A;

s5, mixing the mixed extract B and the auxiliary agent B, slowly adding 70% ethanol at the temperature of 60 ℃, stirring while adding, and adding the agastache rugosus extract B after the temperature is reduced to room temperature;

and S6, combining the mixture obtained in the step S4 and the mixture obtained in the step S5, and then purifying to obtain the disinfectant.

Comparative example 4

The comparative example provides a disinfectant comprising 3 parts by weight of a honeysuckle extract, 10 parts by weight of a dandelion extract, 12 parts by weight of an apple peel extract, 8 parts by weight of a houttuynia cordata extract, 6 parts by weight of a tangerine peel extract, 5 parts by weight of a wrinkled gianthyssop herb extract and an auxiliary agent, wherein the auxiliary agent is prepared from 2 parts by weight of corn starch, 3 parts by weight of polypropylene glycol and 2 parts by weight of carboxymethyl chitosan;

the preparation method of the agastache rugosus extract comprises the following steps of:

s1, weighing agastache rugosus, cleaning to remove impurities, and crushing into coarse powder;

s2, adding 6 times of distilled water into the coarse powder obtained in the step S1, soaking for 20min, and performing reflux extraction for 50 min;

s3, adding 5 times of distilled water into the extract obtained in the step S2, and performing 500W microwave extraction for 30 min;

s4, centrifuging the extract obtained in the step S3 to obtain supernatant, namely the agastache rugosus extract;

the preparation method of the comparative example disinfectant comprises the following steps:

s1, preparing a honeysuckle extract, a dandelion extract, an apple peel extract, a houttuynia cordata extract and a tangerine peel extract, mixing the prepared extracts, and dividing the mixed extracts into two parts, wherein one part is a mixed extract A, and the other part is a mixed extract B;

s2, preparing a wrinkled gianthyssop herb extract, and equally dividing the wrinkled gianthyssop herb extract into two parts, wherein one part is a wrinkled gianthyssop herb extract A, and the other part is a wrinkled gianthyssop herb extract B;

s3, preparing an auxiliary agent, namely dividing the auxiliary agent into two parts, wherein one part is an auxiliary agent A, and the other part is an auxiliary agent B;

s4, mixing the mixed extract A and the auxiliary agent A, slowly adding 60% ethanol at 50 ℃, stirring while adding, cooling to room temperature, and adding the agastache rugosus extract A;

s5, mixing the mixed extract B and the auxiliary agent B, slowly adding 70% ethanol at the temperature of 60 ℃, stirring while adding, and adding the agastache rugosus extract B after the temperature is reduced to room temperature;

and S6, combining the mixture obtained in the step S4 and the mixture obtained in the step S5, and then purifying to obtain the disinfectant.

Comparative example 5

The embodiment provides a disinfectant, which comprises 16 parts by weight of honeysuckle extract, 22 parts by weight of dandelion extract, 20 parts by weight of apple peel extract, 22 parts by weight of houttuynia cordata extract, 15 parts by weight of dried orange peel extract, 16 parts by weight of agastache rugosus extract and an auxiliary agent, wherein the auxiliary agent is prepared from 10 parts by weight of corn starch, 12 parts by weight of polypropylene glycol and 6 parts by weight of carboxymethyl chitosan;

the preparation method of the agastache rugosus extract comprises the following steps of:

s1, weighing agastache rugosus, cleaning to remove impurities, and crushing into coarse powder;

s2, adding 6 times of distilled water into the coarse powder obtained in the step S1, soaking for 20min, and performing reflux extraction for 50 min;

s3, adding 5 times of distilled water into the extract obtained in the step S2, and performing 500W microwave extraction for 30 min;

s4, centrifuging the extract obtained in the step S3 to obtain supernatant, namely the agastache rugosus extract;

the preparation method of the comparative example disinfectant comprises the following steps:

s1, preparing a honeysuckle extract, a dandelion extract, an apple peel extract, a houttuynia cordata extract and a tangerine peel extract, mixing the prepared extracts, and dividing the mixed extracts into two parts, wherein one part is a mixed extract A, and the other part is a mixed extract B;

s2, preparing a wrinkled gianthyssop herb extract, and equally dividing the wrinkled gianthyssop herb extract into two parts, wherein one part is a wrinkled gianthyssop herb extract A, and the other part is a wrinkled gianthyssop herb extract B;

s3, preparing an auxiliary agent, namely dividing the auxiliary agent into two parts, wherein one part is an auxiliary agent A, and the other part is an auxiliary agent B;

s4, mixing the mixed extract A and the auxiliary agent A, slowly adding 60% ethanol at 50 ℃, stirring while adding, cooling to room temperature, and adding the agastache rugosus extract A;

s5, mixing the mixed extract B and the auxiliary agent B, slowly adding 70% ethanol at the temperature of 60 ℃, stirring while adding, and adding the agastache rugosus extract B after the temperature is reduced to room temperature;

and S6, combining the mixture obtained in the step S4 and the mixture obtained in the step S5, and then purifying to obtain the disinfectant.

Comparative example 6

The comparative example provides a disinfectant comprising 10 parts by weight of a honeysuckle extract, 15 parts by weight of a dandelion extract, 16 parts by weight of an apple peel extract, 12 parts by weight of a houttuynia cordata extract, 11 parts by weight of a tangerine peel extract, 8 parts by weight of a wrinkled gianthyssop herb extract and an auxiliary agent, wherein the auxiliary agent is prepared from 6 parts by weight of corn starch, 6 parts by weight of polypropylene glycol and 4 parts by weight of carboxymethyl chitosan;

the preparation method of the agastache rugosus extract comprises the following steps of:

s1, weighing agastache rugosus, cleaning to remove impurities, and crushing into coarse powder;

s2, adding 6 times of distilled water into the coarse powder obtained in the step S1, soaking for 20min, and performing reflux extraction for 50 min;

s3, adding 5 times of distilled water into the extract obtained in the step S2, and performing 500W microwave extraction for 30 min;

s4, centrifuging the extract obtained in the step S3 to obtain supernatant, namely the agastache rugosus extract;

the preparation method of the comparative example disinfectant comprises the following steps:

s1, preparing a honeysuckle extract, a dandelion extract, an apple peel extract, a houttuynia cordata extract and a tangerine peel extract, mixing the prepared extracts, and dividing the mixed extracts into two parts, wherein one part is a mixed extract A, and the other part is a mixed extract B;

s2, preparing a wrinkled giant hyssop extract;

s3, preparing an auxiliary agent, namely dividing the auxiliary agent into two parts, wherein one part is an auxiliary agent A, and the other part is an auxiliary agent B;

s4, mixing the mixed extract A and the auxiliary agent A, slowly adding 60% ethanol at 50 ℃, stirring while adding, and adding the agastache rugosus extract after the temperature is reduced to room temperature;

s5, mixing the mixed extract B and the auxiliary agent B, slowly adding 70% ethanol at the temperature of 60 ℃, stirring while adding, and cooling to room temperature;

and S6, combining the mixture obtained in the step S4 and the mixture obtained in the step S5, and then purifying to obtain the disinfectant.

Comparative example 7

The comparative example provides a disinfectant comprising 10 parts by weight of a honeysuckle extract, 15 parts by weight of a dandelion extract, 16 parts by weight of an apple peel extract, 12 parts by weight of a houttuynia cordata extract, 11 parts by weight of a tangerine peel extract, 8 parts by weight of a wrinkled gianthyssop herb extract and an auxiliary agent, wherein the auxiliary agent is prepared from 6 parts by weight of corn starch, 6 parts by weight of polypropylene glycol and 4 parts by weight of carboxymethyl chitosan;

the preparation method of the agastache rugosus extract comprises the following steps of:

s1, weighing agastache rugosus, cleaning to remove impurities, and crushing into coarse powder;

s2, adding 6 times of distilled water into the coarse powder obtained in the step S1, soaking for 20min, and performing reflux extraction for 50 min;

s3, adding 5 times of distilled water into the extract obtained in the step S2, and performing 500W microwave extraction for 30 min;

s4, centrifuging the extract obtained in the step S3 to obtain supernatant, namely the agastache rugosus extract;

the preparation method of the disinfectant of the comparative example comprises the following steps:

s1, preparing a honeysuckle extract, a dandelion extract, an apple peel extract, a houttuynia cordata extract and a tangerine peel extract, mixing the prepared extracts, and dividing the mixed extracts into two parts, wherein one part is a mixed extract A, and the other part is a mixed extract B;

s2, preparing a wrinkled gianthyssop herb extract, and equally dividing the wrinkled gianthyssop herb extract into two parts, wherein one part is a wrinkled gianthyssop herb extract A, and the other part is a wrinkled gianthyssop herb extract B;

s3, preparing an auxiliary agent;

s4, mixing the mixed extract A and the auxiliary agent A, slowly adding 60% ethanol at 50 ℃, stirring while adding, cooling to room temperature, and adding the agastache rugosus extract A;

s5, slowly adding 70% ethanol into the mixed extract B at the temperature of 60 ℃, stirring while adding, and adding the agastache rugosus extract B after the temperature is reduced to room temperature;

and S6, combining the mixture obtained in the step S4 and the mixture obtained in the step S5, and then purifying to obtain the disinfectant.

Experiment 1: effect test of agastache rugosus extract

And (3) testing a sample: agastache rugosa extracts obtained in example 1 and comparative example 1

The experimental method comprises the following steps:

treating the filter disc: preparing filter disc with diameter of 7mm, sterilizing the filter disc, drying, soaking in herba Agastaches extract for 13 hr, drying, and standing at 4 deg.C for use. Wherein the filter disc was immersed in sterile water as a negative control and the filter disc was immersed in an ampicillin solution as a positive control.

Preparing bacterial liquid: inoculating activated escherichia coli, staphylococcus aureus, bacillus cereus and salmonella in a liquid culture medium for culture, culturing at 37 ℃ for 24 hours, and diluting with sterile water; ② placing the activated streptococcus mutans in an anaerobic box for culturing, culturing for 24h at 37 ℃, and then diluting with sterile water.

And (3) effect measurement: respectively and uniformly inoculating equal amounts of escherichia coli, staphylococcus aureus, bacillus cereus, salmonella and streptococcus mutans to different culture plates. The treated filter discs were placed on a culture plate with 3 parallel of each extract of Agastache rugosa, the plate was placed upside down after the experiment was finished, and after one day of culture at 37 ℃, the bacteriostatic diameter was measured. The experiment is repeated three times, no obvious difference exists between experimental data of each time, and the average value of the experimental data of each time is taken as the final detection result. The final measurement results are shown in table 1 below.

TABLE 1 antibacterial diameter (mm) of Agastache rugosa extract

Item	Escherichia coli	Staphylococcus aureus	Bacillus cereus	Salmonella	Streptococcus mutans
						Negative control	7.00	7.00	7.00	7.00	7.00
Positive control	31.13±0.22	32.31±0.51	30.60±0.30	23.55±1.02	23.01±0.11
						Example 1	7.00	14.23±0.27	15.10±0.31	7.00	7.52±0.23
Comparative example 1	7.00	14.55±0.12	15.21±0.63	7.00	7.85±0.26

As can be seen from table 1: the agastache rugosus extract has no obvious inhibition effect on salmonella and escherichia coli, the agastache rugosus extract has obvious inhibition effect on bacillus cereus, staphylococcus aureus and streptococcus mutans, and the agastache rugosus extract obtained in the example 1 of the invention has equivalent antibacterial effect to the agastache rugosus extract obtained in the comparative example 1.

Experiment 2: effect verification of disinfectants

And (3) testing a sample: disinfectants obtained in examples 2-4 and comparative examples 2-7

The experimental method comprises the following steps:

preparing a strain solution: inoculating activated escherichia coli, staphylococcus aureus, bacillus cereus and salmonella in a liquid culture medium for culture, culturing at 37 ℃ for 24 hours, and diluting by 30 times with sterile water; ② placing the activated streptococcus mutans in an anaerobic box for culturing, culturing for 24h at 37 ℃, and diluting by 30 times with sterile water.

The antibacterial effect of the disinfectant is verified by adopting a test tube method, the disinfectant obtained in the examples 2-4 and the comparative examples 2-7 is diluted to 100 mu g/mL by broth, then the diluted disinfectant is diluted to 10 test tubes in a descending manner, the numbers of the test tubes are respectively tube 1, tube 2, tube 3, tube 4, tube 5, tube 6, tube 7, tube 8, tube 9 and tube 10, then the strain liquid is respectively added to 0.5mL, the culture is carried out at 37 ℃ for 24h, the antibacterial effect is measured, and the measurement result is shown in the following table 2.

TABLE 2 bacteriostatic effect of disinfectant

Note: "-" represents sterile growth; "+" indicates the presence of bacteria.

Likewise, to further illustrate the beneficial effects of the present invention, the following comparative examples are provided:

comparative example 2.1 is provided, which is distinguished from example 4 in that: no dandelion extract is added;

comparative example 2.2 is provided, which is distinguished from example 4 by the following: no apple peel extract is added;

comparative example 2.3 is provided, which comparative example 2.3 differs from example 4 in that: no houttuynia cordata extract is added;

comparative example 2.4 is provided, which comparative example 2.4 differs from example 4 in that: no pericarpium Citri Tangerinae extract is added;

comparative example 2.5 is provided, which is distinguished from example 4 by the following: no addition of auxiliary agent;

the disinfectant prepared by the preparation method disclosed by the invention is verified for bacteriostasis, and the test is carried out according to the method for testing the effect verification of the disinfectant. The antibacterial effects of the disinfectants prepared in comparative example 2.1, comparative example 2.2, comparative example 2.3, comparative example 2.4 and comparative example 2.5 were observed. The results were similar to those in comparative example 2 described above.

Likewise, to further illustrate the beneficial effects of the present invention, the following comparative examples are provided:

comparative example 3.1 is provided, which comparative example 3.1 differs from example 4 in that: no polypropylene glycol was added;

comparative example 3.2 is provided, which comparative example 3.2 differs from example 4 in that: carboxymethyl chitosan is not added;

the disinfectant prepared by the preparation method disclosed by the invention is verified for bacteriostasis, and the test is carried out according to the method for testing the effect verification of the disinfectant. The antibacterial effects of the disinfectants prepared in the comparative examples 3.1 and 3.2 were observed. The results were similar to those in comparative example 3 above.

As can be seen from table 2: the disinfectant has obvious bacteriostatic action on staphylococcus aureus, bacillus cereus and streptococcus mutans in gram-positive bacteria, and also has certain bacteriostatic action on escherichia coli and salmonella in gram-negative bacteria; the antibacterial effect of the disinfectant on escherichia coli and salmonella in gram-negative bacteria is improved by further screening the using amount of each component in the disinfectant. The disinfectant without the formula system has poor bacteriostatic action on staphylococcus aureus, bacillus cereus and streptococcus mutans in gram-positive bacteria and escherichia coli and salmonella in gram-negative bacteria.

It is to be understood that the invention disclosed is not limited to the particular methodology, protocols, and materials described, as these may vary. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments only, and is not intended to limit the scope of the present invention which will be limited only by the appended claims.

It will be understood by those skilled in the art that the foregoing is only a preferred embodiment of the present invention, and is not intended to limit the invention, and that any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the scope of the present invention.

Claims (10)

1. A preparation method of a wrinkled giant hyssop extract is characterized by comprising the following steps:

s1, weighing agastache rugosus, cleaning to remove impurities, and crushing into coarse powder;

s2, adding 6-8 times of distilled water into the coarse powder obtained in the step S1, soaking, and performing reflux extraction;

s3, adding 2-5 times of distilled water into the extract obtained in the step S2, and then performing microwave extraction;

s4, centrifuging the extract obtained in the step S3 to obtain supernatant, namely the agastache rugosus extract.

2. The method as claimed in claim 1, wherein the soaking time of the distilled water in step S2 is 20-30min, and the extraction time of the reflux extraction in step S2 is 50-60 min.

3. The method of claim 1, wherein the microwave extraction in step S3 has a microwave power of 500W and a microwave time of 30 min.

4. An agastache rugosa extract, which is prepared by the preparation method of any one of claims 1 to 3.

5. A disinfectant, characterized in that it comprises the Agastache rugosa extract of claim 4.

6. The disinfectant according to claim 5, wherein the disinfectant further comprises honeysuckle extract, dandelion extract, apple peel extract, houttuynia cordata extract, dried orange peel extract and auxiliaries, wherein the auxiliaries are prepared from corn starch, polypropylene glycol and carboxymethyl chitosan.

7. The disinfectant as recited in claim 6, wherein the weight portion of said corn starch is 3-8, the weight portion of said polypropylene glycol is 5-10, and the weight portion of said carboxymethyl chitosan is 3-5.

8. The disinfectant as recited in claim 6, wherein the honeysuckle extract is 5-15 parts by weight, the dandelion extract is 12-20 parts by weight, the apple peel extract is 13-18 parts by weight, the houttuynia cordata extract is 10-20 parts by weight, the dried orange peel extract is 8-12 parts by weight, and the agastache rugosus extract is 6-15 parts by weight.

9. A method of preparing a disinfectant as claimed in any one of claims 6 to 8, characterised in that said method of preparing comprises the steps of:

s1, preparing a honeysuckle extract, a dandelion extract, an apple peel extract, a houttuynia cordata extract and a tangerine peel extract, mixing the prepared extracts, and dividing the mixed extracts into two parts, wherein one part is a mixed extract A, and the other part is a mixed extract B;

s2, preparing a wrinkled gianthyssop herb extract, and equally dividing the wrinkled gianthyssop herb extract into two parts, wherein one part is a wrinkled gianthyssop herb extract A, and the other part is a wrinkled gianthyssop herb extract B;

s3, preparing an auxiliary agent, namely dividing the auxiliary agent into two parts, wherein one part is an auxiliary agent A, and the other part is an auxiliary agent B;

s4, mixing the mixed extract A and the auxiliary A, slowly adding 60% ethanol at 50-60 ℃, stirring while adding, cooling to room temperature, and adding the agastache rugosus extract A;

s5, mixing the mixed extract B and the auxiliary agent B, slowly adding 70% ethanol at the temperature of 60-70 ℃, stirring while adding, cooling to room temperature, and adding the agastache rugosus extract B;

and S6, combining the mixture obtained in the step S4 and the mixture obtained in the step S5, and then purifying to obtain the disinfectant.

10. Use of a disinfectant according to any one of claims 5 to 8 for disinfection and sterilization and disease control.