CN101731292B - Production method of biopesticide - Google Patents
Production method of biopesticide Download PDFInfo
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- CN101731292B CN101731292B CN 200910272884 CN200910272884A CN101731292B CN 101731292 B CN101731292 B CN 101731292B CN 200910272884 CN200910272884 CN 200910272884 CN 200910272884 A CN200910272884 A CN 200910272884A CN 101731292 B CN101731292 B CN 101731292B
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a production method of biopesticide, comprising the steps of sorting, dipping, enzymolysis and boiling. Specifically, the purchased Chinese herbal medicine with the functions of sterilization, deinsectization and killing pests is separated according to grades; the obtained raw materials are dipped, boiled and/ or performed with enzymolysis to take juice; mixed liquid medicine is obtained by stirring, low-temperature squeezing and filtering as well as high speed centrifugation; after high-pressure filtration and concentration extraction, biopesticide of which the volume concentration is 30-40% is prepared; during dipping, the raw material are dipped in a large pool for 3-5 hours; during boiling, the raw material are boiled at the temperature of 80-100 DEG C; and during high-pressure filtration, mixed liquid medicine is filtered under the pressure which is less than or equal to 100 kg to obtain the filtered liquid medicine. The biopesticide prepared with the method of the invention has no toxicity or harm on people and livestock, does not damage nature, does not pollute environment, can replace chemical and chemical industry synthetic pesticides used at present at home and abroad, can be used for sterilization, deinsectization and killing pests for crops and vegetables.
Description
Technical field
The present invention relates to agricultural chemicals, particularly relate to the production method of biopesticide that a kind of Pesticidal and sterilizing that is widely used in various crops, fruit tree, vegetables is removed the evil.
Background technology
Current international community mainly with the chemical synthetic pesticide desinsection, the damage by disease and insect such as solving crops, fruit tree, vegetables of sterilizing, remove the evil, although chemical synthetic pesticide has solved some problems, but this type of toxicity of pesticide is large, residence time is long, harmful to people and animals, the Nature, air, land, ecotope are produced destructive influences, will bring destructive disaster to the mankind such as long-term use.
Summary of the invention
Technical problem to be solved by this invention is: a kind of production method of biopesticide is provided, the biopesticide of the method preparation is to process through science take China's Chinese traditional herbs material as primary raw material, this agricultural chemicals is nontoxic to people and animals, environmentally friendly, the chemistry of alternative present use, chemical industry synthetic pesticide, be widely used, can satisfy the desinsection to crops, fruit tree, vegetables, flowers, the demand of sterilizing, removing the evil.
The present invention solves its technical problem and adopts following technical scheme:
Production method of biopesticide provided by the invention, comprise select materials, immersion, enzymolysis, boiling operation, the method is: the Chinese herbal medicine medicinal material with sterilization, deinsectization and the function of removing the evil that will buy is told by fine or not grade, the gained raw material by soak, boil and/or enzymolysis after get juice, then obtain the molten admixing medical solutions that is integrated through stirring, cold pressing filtration, high speed centrifugation, again after high-pressure filteration and concentration extraction, make volumetric concentration and be 30～40% biopesticide, wherein: during immersion, raw material was soaked three to five hours with Da Chi; When boiling, raw material is carried out under 80～100 ℃ temperature; During high-pressure filteration, admixing medical solutions is filtered under≤100 kilograms of pressure, obtain filtering liquid medicine.
By weight, described Chinese herbal medicine medicinal material comprise 10 parts of bitter spills, give birth to 5 parts of the tuber of pinellia, 20 parts of Semen Strychnis, 10 parts of mother chrysanthemums, 20 parts of dandelions, give birth to pass through in 10 parts of 5 parts, 3 parts of thin stamens, 3 parts of Chinese ephedra parts and kuh-sengs.Perhaps, the Chinese herbal medicine medicinal material be 10 parts of bitter spills, give birth to 5 parts of the tuber of pinellia, 20 parts of Semen Strychnis, 10 parts of mother chrysanthemums, 10 parts of folium isatidiss, 20 parts of dandelions, give birth to pass through in 10 parts of 5 parts, 20 parts of bunge pricklyash leafs, 3 parts of thin stamens, 3 parts in Chinese ephedra and kuh-sengs.
Above-mentioned cold pressing filters, and its method is: be that the concoction after stirring is squeezed extraction at subzero 4～6 ℃ with under the squeezer, in order to avoid damage the effect of concoction.
During high speed centrifugation, be that the concoction after cold pressing is filtered is equipped with lytic agent, release agent again, after the centrifuge that per minute 5000 turns is centrifugal, obtain the molten admixing medical solutions that is integrated; Described lytic agent is PS43; Described release agent is HS716.
The purposes that above-mentioned biopesticide provided by the invention carries out sterilization, deinsectization and removes the evil in to crops.
Described crops comprise cotton, paddy rice, wheat or fruit tree.
The purposes that above-mentioned biopesticide provided by the invention carries out sterilization, deinsectization and removes the evil in to vegetables.
Described vegetables comprise leaf vegetables, Solanaceae class or rattan section class vegetables.
The present invention compared with prior art has advantages of following main:
One. practical: can produce multiple different classes ofly, Various Functions can be widely used in sterilization, the deinsectization of various crops, fruit tree, vegetables and flowers and removes the evil.Test through carrying out for a long time in enormous quantities physics, field crops, fruit tree, vegetables and to the test of white mouse, this agricultural chemicals has vertical effect of killing to cotton bollworm, yellow tea mite, black cutworm, eggplant fruit borer, large bridging insect pest, damping off, sudden death disease, white star, gray mold, powdery mildew, virus disease etc. there is the prevention killing effect, to the rotten bacterium of mastigomycetes, erythema thinkling sound bacterium, subphylum, partly know that various bacterial parasites such as subphylum bacterium, polypodium aureum, dodder bacterium etc. have inhibitory action.
They are two years old. and pollution-free, nontoxic: the absolute environmental protection of this biopesticide, pollution-free to air, land, natural environment, water and Ge Sheng plant, nontoxic to people and animals, the residence time is in six hours.To white mouse without poisoning and harmless phenomenon.
They are three years old. and raw material are sufficient: more than China's herbal variety, output is big, is the first in the world, and used Chinese medicinal herbs is unrestricted, and price is very cheap, but and establishing in large scale, can satisfy the production of biopesticide and the needs of Future Development fully.
They are four years old. be beneficial to popularization: in certain area factory place, adopt Chinese herbal medicine and the comprehensive batching of China, make and produce biopesticide in enormous quantities, can meet the need of market.
They are five years old. and convenient for production: equipment is simple, available home equipment.
They are six years old. alternative chemistry, the chemical industry synthetic pesticide that uses at present both at home and abroad.
In a word, the biopesticide of the present invention's preparation is to process through science take China's Chinese traditional herbs material as primary raw material, this agricultural chemicals is nontoxic to people and animals, to the Nature do not destroy, free from environmental pollution, can be widely used in sterilization, the deinsectization of various crops, fruit tree, vegetables and flowers and remove the evil.Alternative chemistry, the chemical industry synthetic pesticide that uses at present both at home and abroad.
Embodiment
Production method of biopesticide provided by the invention is to carry out comprehensive production and processing take the traditional Chinese herbal medicine of China as main raw material(s) is equipped with the comprehensive agent centrifugation of lytic agent agent, and the biopesticide of the manufacturing is specific as follows:
Raw material is chosen and is planted, and traditional Chinese medicine can be bought and the open-air harvesting in Chinese medicinal herbs base and mountain region in professional Chinese Medicinal Materials Markets.
Manufacture different traditional Chinese medicines and can adopt different processing methods to process, purpose is to get its juice, adopts the high temperature pressurised machine some medicinal material need to just can be able to be gone out processing of juice at high pressure.The indoor Da Chi that then takes that adopts the pond to soak soaks, and then stirs then to pressurize with agitator and gets juice.And Applied Biotechnology needs the Chinese medicinal herbs of enzymolysis (fermentation) to carry out enzymolysis to some, and enzymolysis is pressed extracting juice after generating again.Then the juice that above-mentioned three kinds of methods is taken out is equipped with the comprehensive agent of lytic agent and centrifugation agent (market all can be bought), and the recycling supercentrifuge carries out the high speed centrifugation running of certain hour.Allow medicine fuse fully decomposition, reach the requirement of scope of design license matter, then carry out sedimentation and filtration, the last work such as concentration extraction.
Described processing method such as some Chinese medicinal herbs must enzymolysis (annotate: multi-level comprehensive fermentation refers to by a kind of rustless steel container, the traditional Chinese medicine of needs processing fermented to generate to the enzymolysis bacterium could take out its juice).And some Chinese herbal medicine must soak the regular hour and could take out its juice under suitable water temperature, however some Chinese herbal medicine need its juice of the complete taking-up of ability in high-temperature boiling a period of time and process.
Described Chinese herbal medicine processing biopesticide method be according to various different Chinese herbal medicines get the juice process principle, need repeatedly enzymolysis (fermentation) such as certain medicinal material, available high-pressure filteration method is taken out its juice that needs as last comprehensive process usefulness behind the enzymolysis.
Described some medicinal material need to soak certain hour, uniform temperature and water capacity what etc. could get the juice that it needs with the high-pressure filteration machine, use as last comprehensive process.
Described some medicinal material need at high temperature boil just can get juice afterwards.Under low pressure could cross its juice that needs of leaching main like this is for the pharmacological that guarantees its concoction is not damaged, and last comprehensive process is used.
Described last comprehensive process is used, that the resulting juice of distinct methods is mixed, and then be equipped with the dissolving intermixture, under supercentrifuge, carry out the high speed centrifugation running in the certain hour, make it all concoctions are molten to be integrated, carry out high-pressure filteration with the centrifugation agent again and could form real biopesticide.
Below in conjunction with the experiment of white mouse and field experiment the effect that biopesticide that the present invention prepares has is described.
One. the experiment of white mouse:
With Chinese herbal medicine biological agricultural chemicals liquid pyloric ligation ulcers rat stomach amount at night, hydrochloric acid in gastric juice and pepsin activity impact are tested.This agricultural chemicals liquid by bitter spill 10 grams, give birth to the tuber of pinellia 5 grams, Semen Strychni 20 grams, mother chrysanthemum 10 grams, folium isatidis 10 grams, dandelion 20 grams, give birth to pass through in 5 grams, bunge pricklyash leaf 20 grams, thin stamen 3 grams, Chinese ephedra 3 grams restrain into kuh-seng 10.
1 materials and methods:
1.1 experiment material:
1.1.1 laboratory animal: healthy wistar rat, body weight are 200 ± 20g, and male and female half and half are purchased from institute of Tongji University of Central China University of Science and Technology zoopery center.
1.1.2 Experimental agents:
Experimental agents adds raw material by Chinese herbal medicine biological agricultural chemicals liquid with institute and the ranitidine sheet forms.
It is thin core 5 grams, kuh-seng 15 grams, bunge pricklyash leaf 30 grams, goldenrod 15 grams, thunder godvine 50 grams, crotons 15 grams, matrine 10 grams, RADIX PAEONIAE ALBA 30 grams and the bletilla striata 20 grams etc. that institute adds raw material.Medicine is provided by wuchang, wuhan district medicine company.Through Biology identification, decoct 30min with distilled water, extract 2 times.Remerge decocting liquid, and the gained liquid is filtered with double gauze, water-soluble heating evaporation is concentrated into and contains crude drug 1g in 100%, the 1ml concoction, puts 1000ml, and sealing is stored in the refrigerator for subsequent use in the vial.
100 of ranitidines, produced lot number by Jiangxi Pharmaceutical Co. Ltd.: 05030201, the time spent adding distil water is made into 0.46% suspension.
1.1.3 key instrument and equipment
LG10-DI refrigerated centrifuge Beijing Medical Centrifugal Machine Factory produces
Constant incubator Plant No. 262 produces
1.2 experimental technique:
1.2.1 animal grouping: by complete Random Design method 50 rats are divided into 5 groups, 10 every group, are respectively: Normal group, heavy dose of group, middle dosage group, small dose group and ranitidine group.
1.2.2 medication: dosage is by the conversion of people and rat corpus surface area (formula: rat day dosage=day for human beings dosage * 0.018), and it always adds the large, medium and small dosage of flavor and is respectively 2 times, 1 times, 0.5 times of the medication dose,equivalent of being grown up.Ranitidine is also converted as stated above and is made the dose,equivalent administration, and all the other groups are fed the physiological saline with same dose.Be normal group 10ml/kg every day, above-mentioned rat gavage every day 2 times, continuous 5 days.
1.2.3 animal is processed and collection of specimens:
Continuously medicine feed is 5 days, can't help water since fasting in the 4th day, after 24 hours, after the perfusion in namely the 5th day 1 hour,, cuts open the belly the ligation pylorus with the animal etherization.Because of behind the rat ligation pylorus 4 hours, stomach midnight secreted and increases the most obviously, and is tending towards constant, therefore after modeling 5 hours, put to death animal with excessive etherization, cut off the abdominal cavity, the ligation spray, downcut full stomach and take out, along the curved osculum of cutting of stomach, filter with double gauze, gastric juice is collected in the graduated centrifuge tube.
1.2.4 observation index:
1.2.4.1 the gastric juice amount, free acid and titration of total acidity: with 3000r/min, draw supernatant and carry out gastric analysis, and record gastric juice amount.Get clear gastric juice 2ml, place small beaker, add each 3 of dimethyl yellow index agent and the agent of phenolphthalein index, mixing, be cherry red, lining one blank sheet of paper splashes into 0.02mol/L sodium hydroxide (NaOH) standard liquid slowly with microburet under small beaker, with dripping with shaking, until cherry red disappears, begin to occur orange, the consumption of record NaOH solution is free acidity terminal point amount, continue with the titration of 0.02mol/LnaOH standard liquid, to the redness that phenolphthalein occurs (blush is not moved back), consumption and the upper used sodium hydroxide sum of record NaOH solution are total acidity terminal point amount, are calculated as follows:
Free acid and total acidity: free acid (mmol/L)=free acidity terminal point amount * 10
Total acidity (mmol/l)=total acidity terminal point amount * 10
1.2.4.2 the detection of pepsin activity: get the conical beaker that gastric juice 1mL puts into 50mL, add 0.05mol/L hydrochloric acid 15m at night L, mixing, put into 2 of protein pipe in every cup and (get the capillary of internal diameter 1mm even thickness, make it fill Fresh Egg with siphonage clear, pipe is interior without bubble, then be placed in 85 degrees centigrade the hot water ware and make protein coagulation, after cold, take out), with the good bottleneck of degreasing tampon, in 37 degrees centigrade of constant incubators, hatch 24h, measure the length (mm) of protein pipe two ends transparent part, ask its mean value with the value of four ends, be calculated as follows:
Pepsin activity: pepsin unit (U)=mean value 2 * 16
1.2.5 statistical procedures:
According to analyzing with the SPSS statistical software, group difference relatively adopts the check relatively of two sample means.
2. result:
Can find out from table 1 analysis, each group of liquid all has inhibitory action to gastric juice amount, free acid, total acid content and pepsin activity.Compare with Normal group and to have highly significant difference (p＜0.01); Inhibitory action to gastric juice amount, free acid, total acid content and pepsin activity strengthens with dosage, and wherein heavy dose of group is compared no significant difference (p＞0.05) with the ranitidine group.
The result shows that Experimental agents has the effect of obvious inhibition rat stomach liquid measure, free acid, total acid content and pepsin activity.Harmless to white mouse simultaneously.
Two. field experiment
Field experiment 1: to cotton, leaflet wood and part cereal crops, the blade of being injured of fruit tree and leaf bud class has carried out Comprehensive Experiment, after mixing, the water of the Chinese herbal medicine biological agricultural chemicals liquid 97% with 3% to the blade of the being injured dispensing of spraying, carries out micro-amplifying observation result such as table 2 after 10～20 minutes.
Field experiment 2: to damping off, sprayed and offer medicine once in dead disease of temper, white star, gray mold, the virus disease position that is injured after the water of the Chinese herbal medicine biological agricultural chemicals liquid 95% with 5% mixed, and it the results are shown in Table 3.
Field experiment 3: the Chinese herbal medicine biological agricultural chemicals liquid with 10% adds 90% water and mixes and spray garden pest the bacterial parasite situation of going out of stopping is seen Table 4.
Subordinate list
Table 1 adds flavor Chinese herbal medicine liquid to the impact (n=10) of rat stomach amount at night, hydrochloric acid in gastric juice and stomach cardia activity
Annotate: compare △ P＜0.05 △ △ P＜0.01 with Normal group
Compare with the ranitidine group, ● P＜0.05 ● ● P＜0.01
Table 2 pair cotton, leaflet wood and part cereal crops, the Comprehensive Experiment of the blade of being injured of fruit tree and leaf bud class
| The insect title | Insect ratio % | Dead ratio % | Death time (minute) | Not dead situation | Synthesis result |
| Two spotted spider mite | 100％ | 70& | 30 | Intoxicating phenomenon is arranged | Dosage |
| Yellow tea mite | 100％ | 70％ | 10 | Nervous centralis is limited | Dosage |
| Cotton bollworm | 100％ | 60％ | 10 | Bradykinesia | Dosage |
| Eggplant fruit borer | 100％ | 90％ | 10 | Bradykinesia | Dosage |
Dead disease of table 3 pair damping off, temper, white star, gray mold, the virus disease result of the test of spraying and offer medicine in the position of being injured
Table 4 sprays garden pest to the bacterial parasite situation of going out of stopping
| The bacterial parasite title | Fungus damage ratio % | Death time (minute) | Dead ratio % | Not dead situation | The result |
| Mastigomycetes | 100％ | 15 | 93 | Intoxicating phenomenon is arranged | Satisfied |
| Erythema thinkling sound bacterium | 100％ | 15 | 91 | Intoxicating phenomenon is arranged | Satisfied |
| The rotten bacterium of subphylum | 100％ | 35 | 91 | Intoxicating phenomenon is arranged | Satisfied |
| Bao Rang bacterium | 100％ | 60 | 74 | Intoxicating phenomenon is arranged | Satisfied |
| The dodder bacterium | 100％ | 100 | 97 | Intoxicating phenomenon is arranged | Satisfied |
Claims (9)
1. production method of biopesticide, comprise select materials, immersion, enzymolysis, boiling operation, it is characterized in that the Chinese herbal medicine medicinal material with sterilization, deinsectization and the function of removing the evil that will buy tells by fine or not grade, the gained raw material by soak, boil and/or enzymolysis after get juice, then obtain the molten admixing medical solutions that is integrated through stirring, cold pressing filtration, high speed centrifugation, again after high-pressure filteration and concentration extraction, make volumetric concentration and be 30～40% biopesticide, wherein: during immersion, raw material was soaked three to five hours with Da Chi; When boiling, raw material is carried out under 80～100 ℃ temperature; During high-pressure filteration, admixing medical solutions is filtered under≤100 kilograms of pressure, obtain filtering liquid medicine;
By weight, described Chinese herbal medicine medicinal material comprise 10 parts of bitter spills, give birth to 5 parts of the tuber of pinellia, 20 parts of Semen Strychnis, 10 parts of mother chrysanthemums, 20 parts of dandelions, give birth to pass through in 10 parts of 5 parts, 3 parts of thin stamens, 3 parts of Chinese ephedra parts and kuh-sengs.
2. production method of biopesticide according to claim 1, it is characterized in that by weight, described Chinese herbal medicine medicinal material is replaced by following medicinal material: 10 parts of bitter spills, give birth to 5 parts of the tuber of pinellia, 20 parts of Semen Strychnis, 10 parts of mother chrysanthemums, 10 parts of folium isatidiss, 20 parts of dandelions, give birth to pass through in 10 parts of 5 parts, 20 parts of bunge pricklyash leafs, 3 parts of thin stamens, 3 parts in Chinese ephedra and kuh-sengs.
3. production method of biopesticide according to claim 1 is characterized in that it is that the concoction after stirring is squeezed extraction at subzero 4～6 ℃ with under the squeezer that cold pressing filters, in order to avoid damage the effect of concoction.
4. production method of biopesticide according to claim 1 when it is characterized in that high speed centrifugation, is that the concoction after cold pressing is filtered is equipped with lytic agent, release agent again, obtains the molten admixing medical solutions that is integrated behind high speed centrifugation.
5. production method of biopesticide according to claim 4 is characterized in that the centrifuge that adopts per minute 5000 to turn carries out high speed centrifugation.
6. the purposes that the biopesticide of the described production method of biopesticide preparation of arbitrary claim carries out sterilization, deinsectization and removes the evil in to crops in the claim 1 to 5.
7. purposes according to claim 6 is characterized in that crops are cotton, paddy rice, wheat or fruit tree.
8. the purposes that the biopesticide of the described production method of biopesticide preparation of arbitrary claim carries out sterilization, deinsectization and removes the evil in to vegetables in the claim 1 to 5.
9. purposes according to claim 8 is characterized in that vegetables are leaf vegetables, Solanaceae class or rattan section class vegetables.
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| CN102428969B (en) * | 2011-11-25 | 2014-01-15 | 上海交通大学 | Plant miticide and preparation method and application thereof |
| CN102812971B (en) * | 2012-08-08 | 2014-12-10 | 山东省农业科学院植物保护研究所 | Compound traditional Chinese medicine extract for killing plant pathogenic bacteria and insect pests |
| CN103396256B (en) * | 2013-08-16 | 2015-09-30 | 诸卫平 | The application of Leaf of Indigowoad decoction liquor in the chemical fertilizer and agricultural chemicals of preparation farm crop |
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| CN104838864B (en) * | 2015-05-22 | 2018-05-11 | 青岛中仁动物药品有限公司 | A kind of novel use of half heel of Chinese herbal medicine |
| CN104838863B (en) * | 2015-05-22 | 2018-05-11 | 青岛中仁药业有限公司 | A kind of method for cultivating specific health-care efficacy crops |
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| CN105685144A (en) * | 2016-03-15 | 2016-06-22 | 王丽军 | Harmless insecticide |
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| CN106577872A (en) * | 2016-11-29 | 2017-04-26 | 青岛海之星生物科技有限公司 | Environment-friendly and efficient red spider mite insecticidal composition and preparation method therefor |
| CN106689229A (en) * | 2016-12-20 | 2017-05-24 | 郭海桥 | Botanical pesticide and preparation method thereof |
| CN107232239A (en) * | 2017-06-08 | 2017-10-10 | 合肥龙滨化工科技有限公司 | A kind of fruits and vegetables biological pesticide composite |
| CN109258717A (en) * | 2018-11-22 | 2019-01-25 | 衡阳仕杰农业发展有限公司 | A kind of organic disinfectant of rice, preparation method and its application method |
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| CN101422176A (en) * | 2008-11-14 | 2009-05-06 | 宁夏农林科学院 | Berberine avermectin water agent |
| CN101692837A (en) * | 2009-11-09 | 2010-04-14 | 王建国 | Pure Chinese medicine disinfectant and process for preparing same |
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| CN101422176A (en) * | 2008-11-14 | 2009-05-06 | 宁夏农林科学院 | Berberine avermectin water agent |
| CN101692837A (en) * | 2009-11-09 | 2010-04-14 | 王建国 | Pure Chinese medicine disinfectant and process for preparing same |
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