CN104026173A - Disinfectant special for inspection instrument in inspection department and preparation method of disinfectant - Google Patents

Abstract

The invention discloses a disinfectant special for an inspection instrument in the inspection department and a preparation method of the disinfectant. The disinfectant is prepared mainly by the following steps of crushing pawpaw, fructus aurantii immaturus, schisandra chinensis, pericarpium citri reticulatae, smoked plum, chinese holly, pomegranate bark, exocarpium, eucalyptus robusta smith, dogwood, folium eriobotryae, selfheal, myrobalan, corn silk, ligusticum wallichii, glossy privet fruits and pericarpium citri reticulatae viride, soaking, heating and reflowing, wherein an auxiliary material is a filter liquor which is prepared by heating poinsettia, radix angelicae, hemp fimble stem-fibre, subprostrate sophora and fungus-infected rice spike with ethanol and extracting, wherein an additive of polyhexamethylene guanidine is added. The disinfectant is faintly acid, and the environmental acidity can be changed, so that most bacteria which grow best within a PH range of 6.0 to 8.0, have the strongest enzymatic activity and strongly grow and breed cannot exist, and the bacterial growth is effectively inhibited; the disinfectant is easily dissolved in water, and the disinfectant is easily washed out without residues after being used; the disinfectant has the advantages of being small in corrosivity, good in stability and simple in preparation process, can be widely applied to disinfecting the inspection instrument in the inspection department and is suitable for mass production.

Description

A kind of clinical laboratory check apparatus appropriative disinfector and preparation method thereof

Technical field

The present invention relates to medical apparatus sterilizing technical field, particularly design a kind of clinical laboratory check apparatus appropriative disinfector and preparation method thereof.

Background technology

Medical inspection section is bearing the task of medical test, must guarantee the accuracy of check, so that clinical laboratory should drop to bacterium scope is minimum, especially checks apparatus, so before check, very important to the sterilization of clinical laboratory's check apparatus.

At present, to the sterilization of check apparatus, be all to use some chemosterilants clinically, with respect to Chinese herbal disinfectant, these disinfectants have certain corrosivity to check apparatus, after sterilizing, article have residual toxicity, need could use after the loose gas of ventilation, and bactericidal effect is subject to the impact of activator concentration and soak time more.

Therefore, a kind of Disinfection Effect good, sterilization after noresidue, to check apparatus the little disinfectant of corrosivity will be very beneficial for the carrying out of the every check work of clinical laboratory.

Summary of the invention

The technical problem that the present invention solves is to provide a kind of clinical laboratory check apparatus appropriative disinfector, this disinfectant is faintly acid, can play good sterilizing effect, material is Chinese herbal medicine, soluble in water, with rear, easily rinses out, noresidue, corrosivity to check apparatus is very little, and manufacture craft is simple, is applicable to a large amount of production.

Another object of the present invention is to provide the preparation method of a kind of clinical laboratory check apparatus appropriative disinfector.

Technical scheme of the present invention is: a kind of clinical laboratory check apparatus appropriative disinfector, mainly to be made by the raw material of following weight portion: take pawpaw 6-8 part, dried immature fruit of citron orange 4-9 part, Schisandra sphenanthera Rehd.et WilS 15-25 part, dried orange peel 18-24 part, dark plum 6-12 part, Chinese holly 14-18 part, granatum 9-18 part, Exocarpium Citri Grandis 6-10 part, eucalyptus robusta 9-20 part, dogwood 5-15 part, loguat leaf 6-15 part, selfheal 4-10 part, myrobalan 5-10 part, corn stigma 4-8 part, Ligusticum wallichii 3-9 part, fruit of glossy privet 6-11 part, rascal 3-6 part, pulverize, by the deionized water of 5-7 times of weight, soak 20-32 hour, backflow 2-3 time, each 1.2-1.5 hour, filter, the filtrate obtaining.

Further, described disinfectant is solution.

Further, the raw material of this disinfectant is comprised of major ingredient, auxiliary material, additive:

First prepare major ingredient: pawpaw 6-8 part, dried immature fruit of citron orange 4-9 part, Schisandra sphenanthera Rehd.et WilS 15-25 part, dried orange peel 18-24 part, dark plum 6-12 part, Chinese holly 14-18 part, granatum 9-18 part, Exocarpium Citri Grandis 6-10 part, eucalyptus robusta 9-20 part, dogwood 5-15 part, loguat leaf 6-15 part, selfheal 4-10 part, myrobalan 5-10 part, corn stigma 4-8 part, Ligusticum wallichii 3-9 part, fruit of glossy privet 6-11 part, rascal 3-6 part, by formula ratio, take above-mentioned raw materials, put into cracker and be ground into meal, by the deionized water of 5-7 times of weight, soak 20-32 hour, water temperature is controlled at 25-30 ℃, add hot reflux 2-3 time, each 1.2-1.5 hour, filter, obtain filtrate, standby,

Next prepares auxiliary material: poinsettia 2-6 part, root of Dahurain angelica 6-10 part, numb skin 2-8 part, subprostrate sophora 5-12 part, fungus-infected rice spike 3-8 part, by formula ratio, take above-mentioned auxiliary material, microwave 5-10 minute, grinds into powder, and 80 orders sieve, obtain fine powder, 80% alcohol solution dipping 5-6 hour of 3-5 times of weight for the fine powder making, 2-3 hour is extracted in heating, reclaims ethanol, filter, obtain filtrate for later use;

In addition, take 0.15-0.25 part additive stand-by, described additive is poly (hexamethylene) acid, and its degree of polymerization is greater than 2000;

Finally, the above-mentioned major ingredient making by proportioning, auxiliary material, additive are mixed, stir, add water, according to 1: dilution proportion (1000-1200), regulate pH to 5.0-6.0, obtain disinfectant.

Another object of the present invention is to provide the preparation method of a kind of clinical laboratory check apparatus appropriative disinfector, and the preparation method of described disinfectant comprises the following steps:

(1) by formula ratio, take pawpaw 6-8 part, dried immature fruit of citron orange 4-9 part, Schisandra sphenanthera Rehd.et WilS 15-25 part, dried orange peel 18-24 part, dark plum 6-12 part, Chinese holly 14-18 part, granatum 9-18 part, Exocarpium Citri Grandis 6-10 part, eucalyptus robusta 9-20 part, dogwood 5-15 part, loguat leaf 6-15 part, selfheal 4-10 part, myrobalan 5-10 part, corn stigma 4-8 part, Ligusticum wallichii 3-9 part, fruit of glossy privet 6-11 part, rascal 3-6 part;

(2) put into cracker and be ground into meal;

(3) by the deionized water of 5-7 times of weight, soak 20-32 hour, water temperature is controlled at 25-30 ℃;

(4) add hot reflux 2-3 time, each 1.2-1.5 hour;

(5) filter, obtain filtrate, be described disinfectant.

Further, the preparation method of described a kind of clinical laboratory check apparatus appropriative disinfector, comprises the following steps:

(1) prepare major ingredient: 7 parts of pawpaws, 6.5 parts of the dried immature fruit of citron oranges, 20 parts of Schisandra sphenanthera Rehd.et WilS, 21 parts of dried orange peels, 9 parts of dark plums, 16 parts of Chinese hollies, 13.5 parts of granatums, 8 parts of Exocarpium Citri Grandises, 14.5 parts of eucalyptus robusta, 10 parts of dogwood, 10.5 parts of loguat leaves, 7 parts of selfheals, 7.5 parts of myrobalans, 6 parts of corn stigmas, 6 parts of Ligusticum wallichiis, 8.5 parts of the fruit of glossy privets, 4.5 parts, rascal, by formula ratio, take above-mentioned raw materials, pulverize, by the deionized water of 6 times of weight, soak 26 hours, water temperature is controlled at 27.5 ℃, add hot reflux 2 times, each 1.35 hours, filter, obtain filtrate, standby;

(2) prepare auxiliary material: 4 parts of poinsettia, 8 parts of the roots of Dahurain angelica, 5 parts of numb skins, 8.5 parts of subprostrate sophoras, 5.5 parts of fungus-infected rice spikes, by formula ratio, take above-mentioned auxiliary material, microwave 7.5 minutes, grinds into powder, and 80 orders sieve, obtain fine powder, 80% alcohol solution dipping of 4 times of weight 5.5 hours for the fine powder making, heating is extracted 2.5 hours, reclaims ethanol, filter, obtain filtrate for later use;

(3) take 0.20 part of additive stand-by, described additive is poly (hexamethylene) acid, and its degree of polymerization is greater than 2000;

(4) the above-mentioned major ingredient making by proportioning, auxiliary material, additive are mixed, stir, then add water, according to the dilution proportion of 1: 1100, regulate pH to 5.5, obtain disinfectant.

Compared with prior art, beneficial effect of the present invention is embodied in:

First, the Chinese medicinal pawpaw that the present invention chooses, the dried immature fruit of citron orange, Schisandra sphenanthera Rehd.et WilS, dried orange peel, dark plum, Chinese holly, granatum, Exocarpium Citri Grandis, eucalyptus robusta, dogwood, loguat leaf, selfheal, myrobalan, corn stigma, Ligusticum wallichii, the fruit of glossy privet, in rascal, all contain organic acid, as: malic acid, tartaric acid, citric acid, eucalyptus robusta acid, gallic acid and vitamin C, in these acids molecules, all contain two above carboxyls and open hydroxyl or phenolic hydroxyl group, therefore acid stronger than corresponding organic carboxyl acid, so there is antibacterial action, most of bacteriums are best in the growth of pH6.0-8.0 scope, the activity of its enzyme is also the strongest, growth and breeding is vigorous, as be placed in sour environment, most germs can not be survived, therefore these acid medicines can change environment acidity, play bactericidal action.

Pawpaw property is put down, is slightly cold, taste is sweet, has stronger antibacterial action, and multiple enteron aisle bacterium and staphylococcus are had to remarkable inhibitory action, and Diplococcus pneumopniae and tubercle bacillus are had to obvious inhibitory action, and cydonic acid has inhibitory action to Qiang's parasitosis Richettsia, the dried immature fruit of citron orange is bitter and be slightly cold, and contains palmitic acid, stearic acid, oleic acid, linoleic acid, linolenic acid, goes the acetic acid mesityl oxide that makes a noise, different lemon Buddhist nun acid etc., good anti-bacterial effect, Schisandra sphenanthera Rehd.et WilS contains citric acid, malic acid, tartaric acid, succinic acid, and its extract liquid all has inhibitory action to bacillus anthracis, staphylococcus aureus, staphylococcus albus, typhoid bacillus, comma bacillus etc. in vitro, dried orange peel is bitter, pungent, temperature, and the Nobiletin containing has anti-inflammatory, antiviral, anticancer, anti-sudden change, antiallergy, antiulcer, analgesic activity, dark plum acid, puckery, flat, contain dark plum containing citric acid 19%, malic acid 15%, succinic acid, carbohydrate, sitosterol, ceroidlike material and oleanolic acid sample material, to bacillus anthracis, diphtheria and diphtheroid, hay bacillus, staphylococcus (golden yellow, lemon yellow, white), pneumococcus all has inhibitory action, to Escherichia coli, bacterium sonnei, proteus, Typhoid and paratyphoid bacillus, Pseudomonas aeruginosa, in the intestines such as comma bacillus, pathogenic bacteria are also effective, to Escherichia coli, the various bacteria such as staphylococcus have inhibitory action, also have. anti-allergic effects, Chinese holly is bitter, puckery, cool, the dilute aqueous solution that is equivalent to crude drug 0.0125g/ml all has inhibitory action to Pseudomonas aeruginosa, Escherichia coli, typhoid bacillus, shigella flexneri, Bacillus alcaligenes, hay bacillus, staphylococcus aureus, when the aqueous solution is diluted to while being equivalent to crude drug 0.0031g/ml, staphylococcus aureus is still had to inhibitory action, granatum is sour and astringent, temperature, contain malic acid, pectin, eucalyptus robusta acid, the 4 clock shigella dysenteriaes such as in will he, Amur, Fu Shi and Song are all had to antibacterial action, the multiple thin mattresses such as tubercle bacillus, Escherichia coli, proteus, Pseudomonas aeruginosa all have inhibitory action, to Jin Shi trichophyta, the various skin fungies such as proper alignment hair tinea mattress all have the inhibitory action of the degree of paying no attention to, Exocarpium Citri Grandis contains Nobiletin, vitamin C, eucalyptus robusta dispelling wind is antipyretic, and inhibiting bacteria and diminishing inflammation is anticorrosion antipruritic, contains gallic acid, for skin degerming, dogwood can suppress the growth of staphylococcus aureus in vitro, and typhoid fever, dysentery bacterium are had to inhibitory action.Water logging agent (1: 3) has inhibitory action in various degree to trichophyton in vitro; Loguat leaf is bitter, is slightly cold, and white or staphylococcus aureus, Diplococcus pneumopniae, shigella flexneri are all had to antibacterial action, has antiinflammatory action; Selfheal is cold in nature, sweet, pungent, the micro-hardship of taste, there is resisting pathogenic microbes effect, staphylococcus aureus, beta hemolytic streptococcus, Escherichia coli, typhoid bacillus, shigella dysenteriae, diphtheria bacillus, bacillus anthracis, Pseudomonas aeruginosa all have inhibitory action, and water logging agent (1: 4) is also had inhibitory action in various degree to permitting some common pathogenic dermatophyte such as blue achorion, Du's big belly microspore achorion difficult to understand in vitro; Myrobalan is effective to various shigella dysenteriaes, and stronger to Pseudomonas aeruginosa, diphtheria bacillus effect, and staphylococcus aureus, Escherichia coli, pneumococcus, hemolytic streptococcus, proteus, bacillus typhi murium are also had to effect; The valid density of shigella dysenteriae, staphylococcus aureus, Pseudomonas aeruginosa is respectively 1: 32,1: 128,1: 64.Myrobalan has the effect of good anti-typhoid bacillus in vitro.With the ethanol extract of hydrochloric acid, extracted by ether, there is higher antibacterial and antifungic action; Corn stigma contains and enriches malic acid, citric acid, tartaric acid, vitamin C, has bactericidal antiphlogistic effect; Ligusticum wallichii has inhibitory action, Ligusticum wallichii water logging agent (1: 3) some pathogenic dermatophyte also to be had to inhibitory action in vitro to large intestine, dysentery (Song Nei Shi), distortion, green pus, typhoid fever, paratyphosum Bacterium and comma bacillus etc.; The fruit of glossy privet has antibacterial action; Rascal contains Nobiletin, can anti-inflammatory, antiviral, anticancer, anti-sudden change, antiallergy, antiulcer.

The second, the Chinese medicine poinsettia that the present invention chooses, the root of Dahurain angelica, numb skin, subprostrate sophora, fungus-infected rice spike, be used in conjunction with aforesaid medicine, further strengthened antimicrobial antiphlogistic effect; Poly (hexamethylene) acid has sterilization wide spectrum, and valid density is low, and speed of action is fast, stable in properties, soluble in water, can use at normal temperatures, for a long time antibacterial, have no side effect, non-corrosiveness, colourless, odorless, nontoxic, do not fire, not quick-fried, use safety, moderate premium properties, especially its applicable pH value width is large, so very applicable and above-mentioned, be weakly acidic Chinese herbal medicine compatibility, prepare the splendid disinfection sanitizer of effect.

The 3rd, disinfectant good stability of the present invention, soluble in water, after sterilization, easily rinse out, noresidue, very little to the corrosiveness of check apparatus, preparation technology is simple, is applicable to a large amount of production.

Embodiment

Below in conjunction with specific embodiment and test, the present invention is described in more detail

Specific embodiment

Embodiment 1:

Clinical laboratory's check apparatus appropriative disinfector, is comprised of following raw material by weight: 6 parts of pawpaws, 4 parts of the dried immature fruit of citron oranges, 15 parts of Schisandra sphenanthera Rehd.et WilS, 18 parts of dried orange peels, 6 parts of dark plums, 14 parts of Chinese hollies, 9 parts of granatums, 6 parts of Exocarpium Citri Grandises, 9 parts of eucalyptus robusta, 5 parts of dogwood, 6 parts of loguat leaves, 4 parts of selfheals, 5 parts of myrobalans, 4 parts of corn stigmas, 3 parts of Ligusticum wallichiis, 6 parts of the fruit of glossy privets, 3 parts, rascal, 2 parts of poinsettia, 6 parts of the roots of Dahurain angelica, 2 parts of numb skins, 5 parts of subprostrate sophoras, 3 parts of fungus-infected rice spikes, 0.15 part of poly (hexamethylene) acid.

Preparation technology is:

(1) prepare major ingredient: 6 parts of pawpaws, 4 parts of the dried immature fruit of citron oranges, 15 parts of Schisandra sphenanthera Rehd.et WilS, 18 parts of dried orange peels, 6 parts of dark plums, 14 parts of Chinese hollies, 9 parts of granatums, 6 parts of Exocarpium Citri Grandises, 9 parts of eucalyptus robusta, 5 parts of dogwood, 6 parts of loguat leaves, 4 parts of selfheals, 5 parts of myrobalans, 4 parts of corn stigmas, 3 parts of Ligusticum wallichiis, 6 parts of the fruit of glossy privets, 3 parts, rascal, by formula ratio, take above-mentioned raw materials, put into cracker and be ground into meal, by the deionized water of 5 times of weight, soak 20 hours, water temperature is controlled at 25 ℃, add hot reflux 2 times, each 1.2 hours, filter, obtain filtrate, standby;

(2) prepare auxiliary material: 2 parts of poinsettia, 6 parts of the roots of Dahurain angelica, 2 parts of numb skins, 5 parts of subprostrate sophoras, 3 parts of fungus-infected rice spikes, by formula ratio, take above-mentioned auxiliary material, microwave 5 minutes, grinds into powder, and 80 orders sieve, obtain fine powder, 80% alcohol solution dipping of 3 times of weight 5 hours for the fine powder making, heating is extracted 2 hours, reclaims ethanol, filter, obtain filtrate for later use;

(3) take 0.15 part of additive stand-by, described additive is poly (hexamethylene) acid, and its degree of polymerization is greater than 2000;

(4) the above-mentioned major ingredient making by proportioning, auxiliary material, additive are mixed, stir, add water, according to the dilution proportion of 1: 1000, regulate pH to 5.0, obtain disinfectant.

Embodiment 2

Clinical laboratory's check apparatus appropriative disinfector, is comprised of following raw material by weight: 7 parts of pawpaws, 6.5 parts of the dried immature fruit of citron oranges, 20 parts of Schisandra sphenanthera Rehd.et WilS, 21 parts of dried orange peels, 9 parts of dark plums, 16 parts of Chinese hollies, 13.5 parts of granatums, 8 parts of Exocarpium Citri Grandises, 14.5 parts of eucalyptus robusta, 10 parts of dogwood, 10.5 parts of loguat leaves, 7 parts of selfheals, 7.5 parts of myrobalans, 6 parts of corn stigmas, 6 parts of Ligusticum wallichiis, 8.5 parts of the fruit of glossy privets, 4.5 parts, rascal, 4 parts of poinsettia, 8 parts of the roots of Dahurain angelica, 5 parts of numb skins, 8.5 parts of subprostrate sophoras, 5.5 parts of fungus-infected rice spikes, 0.20 part of poly (hexamethylene) acid.

Preparation technology is:

(1) prepare major ingredient: 7 parts of pawpaws, 6.5 parts of the dried immature fruit of citron oranges, 20 parts of Schisandra sphenanthera Rehd.et WilS, 21 parts of dried orange peels, 9 parts of dark plums, 16 parts of Chinese hollies, 13.5 parts of granatums, 8 parts of Exocarpium Citri Grandises, 14.5 parts of eucalyptus robusta, 10 parts of dogwood, 10.5 parts of loguat leaves, 7 parts of selfheals, 7.5 parts of myrobalans, 6 parts of corn stigmas, 6 parts of Ligusticum wallichiis, 8.5 parts of the fruit of glossy privets, 4.5 parts, rascal, by formula ratio, take above-mentioned raw materials, put into cracker and be ground into meal, by the deionized water of 6 times of weight, soak 26 hours, water temperature is controlled at 27.5 ℃, add hot reflux 2 times, each 1.35 hours, filter, obtain filtrate, standby;

(2) prepare auxiliary material: 4 parts of poinsettia, 8 parts of the roots of Dahurain angelica, 5 parts of numb skins, 8.5 parts of subprostrate sophoras, 5.5 parts of fungus-infected rice spikes, by formula ratio, take above-mentioned auxiliary material, microwave 7.5 minutes, grinds into powder, and 80 orders sieve, obtain fine powder, 80% alcohol solution dipping of 4 times of weight 5.5 hours for the fine powder making, heating is extracted 2.5 hours, reclaims ethanol, filter, obtain filtrate for later use;

(3) take 0.20 part of additive stand-by, described additive is poly (hexamethylene) acid, and its degree of polymerization is greater than 2000;

(4) the above-mentioned major ingredient making by proportioning, auxiliary material, additive are mixed, stir, add water, according to the dilution proportion of 1: 1100, regulate pH to 5.5, obtain disinfectant.

Embodiment 3:

Clinical laboratory's check apparatus appropriative disinfector, is comprised of following raw material by weight: 8 parts of pawpaws, 9 parts of the dried immature fruit of citron oranges, 25 parts of Schisandra sphenanthera Rehd.et WilS, 24 parts of dried orange peels, 12 parts of dark plums, 18 parts of Chinese hollies, 18 parts of granatums, 10 parts of Exocarpium Citri Grandises, 20 parts of eucalyptus robusta, 15 parts of dogwood, 15 parts of loguat leaves, 10 parts of selfheals, 10 parts of myrobalans, 8 parts of corn stigmas, 9 parts of Ligusticum wallichiis, 11 parts of the fruit of glossy privets, 6 parts, rascal, 6 parts of poinsettia, 10 parts of the roots of Dahurain angelica, 8 parts of numb skins, 12 parts of subprostrate sophoras, 8 parts of fungus-infected rice spikes, 0.25 part of poly (hexamethylene) acid.

Preparation technology is:

(1) prepare major ingredient: 8 parts of pawpaws, 9 parts of the dried immature fruit of citron oranges, 25 parts of Schisandra sphenanthera Rehd.et WilS, 24 parts of dried orange peels, 12 parts of dark plums, 18 parts of Chinese hollies, 18 parts of granatums, 10 parts of Exocarpium Citri Grandises, 20 parts of eucalyptus robusta, 15 parts of dogwood, 15 parts of loguat leaves, 10 parts of selfheals, 10 parts of myrobalans, 8 parts of corn stigmas, 9 parts of Ligusticum wallichiis, 11 parts of the fruit of glossy privets, 6 parts, rascal, by formula ratio, take above-mentioned raw materials, put into cracker and be ground into meal, by the deionized water of 7 times of weight, soak 32 hours, water temperature is controlled at 30C, add hot reflux 3 times, each 1.5 hours, filter, obtain filtrate, standby;

(2) prepare auxiliary material: 6 parts of poinsettia, 10 parts of the roots of Dahurain angelica, 8 parts of numb skins, 12 parts of subprostrate sophoras, 8 parts of fungus-infected rice spikes, by formula ratio, take above-mentioned auxiliary material, microwave 10 minutes, grinds into powder, and 80 orders sieve, obtain fine powder, 80% alcohol solution dipping of 5 times of weight 6 hours for the fine powder making, heating is extracted 3 hours, reclaims ethanol, filter, obtain filtrate for later use;

(3) take 0.25 part of additive stand-by, described additive is poly (hexamethylene) acid, and its degree of polymerization is greater than 2000;

(4) the above-mentioned major ingredient making by proportioning, auxiliary material, additive are mixed, stir, add water, according to the dilution proportion of 1: 1200, regulate pH to 6.0, obtain disinfectant.

Bacteriostatic experiment

Disinfectant of the present invention is carried out to bacteriostatic experiment below:

1, for examination bacterial classification:

Select the representative bacterial classification in laboratory, bacillus subtilis, staphylococcus aureus and Escherichia coli, all purchased from Products in China calibrating institute.

2, bacterium culture medium: nutrient agar and beef extract-peptone solid culture medium.

3, experiment classification:

This bacteriostatic experiment is divided into two groups, is respectively experimental group and control group, and experimental group is selected disinfectant of the present invention, and control group selects Iodophor as disinfectant.

4, specific experiment step:

(1) prepare bacterium liquid: above-mentioned three kinds of bacterial classifications are inoculated in respectively on three agar mediums, at 37 ℃, cultivate 32 hours, from above-mentioned agar medium, picking colonies typical is inoculated in respectively in three fresh nutrient broth mediums respectively, synthermal under, cultivate 32 hours, standby;

(2) get each 45ml of disinfectant of the present invention and iodophor disinfectant, be placed in respectively cut-back tank, it is diluted respectively to 30 times, above-mentioned two kinds of dilution solution are placed in respectively in two containers, be placed under aseptic condition, standby;

(3) experimental strain of above-mentioned steps (1) being cultivated 32 hours through nutrient broth medium dilutes respectively 50 times with aseptic nutrient broth, and every kind of bacterial classification is sub-packed in two culture dishes, respectively mark control group and experimental group;

(4) dilute solution of two parts of disinfectants of the present invention of above-mentioned steps (2) gained is splashed into respectively to three culture dishes of experimental group, two parts of iodophor disinfectant dilute solutions splash into respectively three culture dishes of control group.

5, experimental observation is measured:

Above-mentioned experiment is observed, observed three groups of bacterial classifications and measure bacterial classification revolution mark concrete outcome.

6, after observation is measured, above-mentioned culture dish is all placed under aseptic condition standing two days, to be sterilized dose completely after volatilization, tracing observation finds, control group bacterium colony continues breeding, and experimental group bacterium colony is unchanged.

7, experimental result:

The sterilizing ability of experimental group disinfectant of the present invention is much larger than the sterilizing ability of control group disinfectant, and also very remarkable to the inhibitory action of all kinds of bacterial classifications.

Finally it should be noted that: above embodiment only, in order to technical scheme of the present invention to be described, is not intended to limit; Although the present invention is had been described in detail with reference to previous embodiment, those of ordinary skill in the art is to be understood that: its technical scheme that still can record previous embodiment is modified, or part technical characterictic is wherein equal to replacement; And these modifications or replacement do not make the essence of appropriate technical solution depart from the spirit and scope of embodiment of the present invention technical scheme.

Claims (5)

1.Yi Zhong clinical laboratory check apparatus appropriative disinfector, it is characterized in that it being mainly that raw material by following weight portion is made: pawpaw 6-8 part, dried immature fruit of citron orange 4-9 part, Schisandra sphenanthera Rehd.et WilS 15-25 part, dried orange peel 18-24 part, dark plum 6-12 part, Chinese holly 14-18 part, granatum 9-18 part, Exocarpium Citri Grandis 6-10 part, eucalyptus robusta 9-20 part, dogwood 5-15 part, loguat leaf 6-15 part, selfheal 4-10 part, myrobalan 5-10 part, corn stigma 4-8 part, Ligusticum wallichii 3-9 part, fruit of glossy privet 6-11 part, rascal 3-6 part, pulverize, by deionized water, soak, reflux, filter the filtrate obtaining.

2. a kind of clinical laboratory according to claim 1 check apparatus appropriative disinfector, is characterized in that described disinfectant is solution.

3. a kind of clinical laboratory according to claim 1 check apparatus appropriative disinfector, is characterized in that: the raw material of this disinfectant is comprised of major ingredient, auxiliary material, additive;

First prepare major ingredient: pawpaw 6-8 part, dried immature fruit of citron orange 4-9 part, Schisandra sphenanthera Rehd.et WilS 15-25 part, dried orange peel 18-24 part, dark plum 6-12 part, Chinese holly 14-18 part, granatum 9-18 part, Exocarpium Citri Grandis 6-10 part, eucalyptus robusta 9-20 part, dogwood 5-15 part, loguat leaf 6-15 part, selfheal 4-10 part, myrobalan 5-10 part, corn stigma 4-8 part, Ligusticum wallichii 3-9 part, fruit of glossy privet 6-11 part, rascal 3-6 part, by formula ratio, take above-mentioned raw materials, pulverize, by the deionized water of 5-7 times of weight, soak 20-32 hour, backflow 2-3 time, each 1.2-1.5 hour, filter, obtain filtrate, standby;

Next prepares auxiliary material: poinsettia 2-6 part, root of Dahurain angelica 6-10 part, numb skin 2-8 part, subprostrate sophora 5-12 part, fungus-infected rice spike 3-8 part, by formula ratio, take above-mentioned auxiliary material, microwave 5-10 minute, grinds into powder, and 80 orders sieve, obtain fine powder, 80% alcohol solution dipping 5-6 hour of 3-5 times of weight for the fine powder making, 2-3 hour is extracted in heating, reclaims ethanol, filter, obtain filtrate for later use;

In addition, take 0.15-0.25 part additive stand-by, described additive is poly (hexamethylene) acid;

Finally, the above-mentioned major ingredient making by proportioning, auxiliary material, additive are mixed, stir, add water, according to 1: dilution proportion (1000-1200), regulate pH to 5.0-6.0, obtain disinfectant.

The preparation method of 4.Yi Zhong clinical laboratory check apparatus appropriative disinfector, is characterized in that comprising the following steps:

(1) by formula ratio, take pawpaw 6-8 part, dried immature fruit of citron orange 4-9 part, Schisandra sphenanthera Rehd.et WilS 15-25 part, dried orange peel 18-24 part, dark plum 6-12 part, Chinese holly 14-18 part, granatum 9-18 part, Exocarpium Citri Grandis 6-10 part, eucalyptus robusta 9-20 part, dogwood 5-15 part, loguat leaf 6-15 part, selfheal 4-10 part, myrobalan 5-10 part, corn stigma 4-8 part, Ligusticum wallichii 3-9 part, fruit of glossy privet 6-11 part, rascal 3-6 part;

(2) put into cracker and be ground into meal;

(3) by the deionized water of 5-7 times of weight, soak 20-32 hour;

(4) backflow is 2-3 time, each 1.2-1.5 hour;

(5) filter, obtain filtrate, be described disinfectant.

5. a kind of clinical laboratory according to claim 4 checks the preparation method of apparatus appropriative disinfector, it is characterized in that comprising the following steps:

(1) prepare major ingredient: 7 parts of pawpaws, 6.5 parts of the dried immature fruit of citron oranges, 20 parts of Schisandra sphenanthera Rehd.et WilS, 21 parts of dried orange peels, 9 parts of dark plums, 16 parts of Chinese hollies, 13.5 parts of granatums, 8 parts of Exocarpium Citri Grandises, 14.5 parts of eucalyptus robusta, 10 parts of dogwood, 10.5 parts of loguat leaves, 7 parts of selfheals, 7.5 parts of myrobalans, 6 parts of corn stigmas, 6 parts of Ligusticum wallichiis, 8.5 parts of the fruit of glossy privets, 4.5 parts, rascal, by formula ratio, take above-mentioned raw materials, pulverize, by the deionized water of 6 times of weight, soak 26 hours, reflux 2 times, each 1.3 hours, filter, obtain filtrate, standby;

(2) prepare auxiliary material: 4 parts of poinsettia, 8 parts of the roots of Dahurain angelica, 5 parts of numb skins, 8.5 parts of subprostrate sophoras, 5.5 parts of fungus-infected rice spikes, by formula ratio, take above-mentioned auxiliary material, microwave 7 minutes, grinds into powder, and 80 orders sieve, obtain fine powder, 80% alcohol solution dipping of 4 times of weight 5.5 hours for the fine powder making, heating is extracted 2.5 hours, reclaims ethanol, filter, obtain filtrate for later use;

(3) take 0.20 part of additive stand-by, described additive is poly (hexamethylene) acid;

(4) the above-mentioned major ingredient making by proportioning, auxiliary material, additive are mixed, stir, then add water, according to the dilution proportion of 1: 1100, regulate pH to 5.5, obtain disinfectant.