CN112353867A

CN112353867A - Natural plant bacteriostatic spray and preparation method thereof

Info

Publication number: CN112353867A
Application number: CN202011096998.1A
Authority: CN
Inventors: 王乃龙; 李耀钊; 陈健超
Original assignee: Mentholatum China Pharmaceuticals Co Ltd
Current assignee: Mentholatum China Pharmaceuticals Co Ltd
Priority date: 2020-10-14
Filing date: 2020-10-14
Publication date: 2021-02-12

Abstract

The invention discloses a natural plant bacteriostatic spray and a preparation method thereof, the spray does not contain chemical components for disinfection and bacteriostasis, does not contain ethanol, and is made mild, safe and non-irritant by adding natural plant components (tiled white bead leaf extract, white willow bark extract, sage herb leaf extract and peony root bark extract) with antibacterial property and two polyols (pentanediol and 1, 3-propanediol) with bacteriostatic action, and through mutual cooperation, the natural plant bacteriostatic spray is safe and highly effective in bacteriostasis without damaging skin barriers and human skins.

Description

Natural plant bacteriostatic spray and preparation method thereof

Technical Field

The invention relates to the technical field of disinfection reagents, in particular to a natural plant bacteriostatic spray and a preparation method thereof.

Background

The world is influenced by the epidemic situation of the new coronavirus in 2020, and the demand of consumers for the disinfection products is increased explosively. At present, the mainstream antibacterial disinfection products in the market mainly comprise chemical disinfection components, such as ethanol, chlorine-containing disinfectant, quaternary ammonium salt disinfectant, chlorine dioxide, phenol disinfectant, silver ions, hypochlorous acid, iodine agent and the like.

Although the chemical disinfectants are very powerful, the chemical disinfectants are not natural and safe, have the stimulation effect on human bodies, are smelly, have strong corrosivity, and are easy to generate drug resistance after long-term use.

Disclosure of Invention

In order to overcome the defects of the prior art, the invention aims to provide a natural plant bacteriostatic spray and a preparation method thereof. It is suitable for hand, face and other parts to inhibit bacteria in skin to certain extent, reduce the risk of spreading diseases to other people and protect individual from infection. The hand sanitizer is mainly used for surgical hand disinfection, personal hygiene hand disinfection, skin disinfection, mucous membrane disinfection and the like.

The purpose of the invention is realized by adopting the following technical scheme:

a preparation method of a natural plant bacteriostatic spray comprises the following steps:

1) mixing the white willow bark extract with 1, 3-propylene glycol to obtain 1, 3-propylene glycol solution of white willow bark extract to obtain a pre-prepared solution; since the solubility of the white willow bark extract in water is only 0.3%, while the solubility in 1, 3-propanediol is 3% and the solubility in 1, 3-propanediol is the highest, the 1, 3-propanediol is selected as the solvent for the white willow bark extract, which can greatly simplify the production time and risk.

2) Adding arginine into purified water, adjusting the pH value to 6.5-7.5, adding a pentanediol solution of the beautyberry leaf extract, stirring until the extract is completely dissolved, and adjusting the pH value to 3.5-6.0; because the spreading of the beautyberry leaf extract is white powder, the beautyberry leaf extract can not be directly dissolved in water but only be dissolved in alkalescent solution,

3) adding the prefabricated liquid obtained in the step 1) and the sage herb leaf extract into the solution obtained in the step 2), stirring and dissolving, and then adjusting the pH value to 3.5-4.0 to obtain a natural plant bacteriostatic spray; the higher the pH of the natural plant bacteriostatic spray is, the poorer the bacteriostatic effect is, so that the bacteriostatic effect is the best when the pH is 3.5-4.0, and if the strong alkaline pH is greater than 8, the natural plant bacteriostatic spray loses the bacteriostatic ability.

The natural plant bacteriostatic spray comprises the following raw materials in percentage by weight: 1-20% of pentanediol, 1, 3-propylene glycol, 1-30% of tiled folium gaultheriae yunnanensis extract, 0.01-2% of white willow bark extract, 0.1-2% of salvia officinalis leaf extract, 0.1-4% of peony root bark extract, 0.1-0.4% of arginine and the balance of purified water.

Further, in the step 1), the concentration of the white willow bark extract in the 1, 3-propanediol solution of the white willow bark extract is 1%.

Further, in the step 2), EDTA is used for adjusting the pH value to 3.5-6.0.

Further, in the step 3), EDTA is used for adjusting the pH value to 3.5-4.0.

Still further, in steps 1) to 3), the reaction temperature does not exceed 60 ℃. Because all bacteriostatic components of the invention are natural plants or natural plant sources, a low-temperature blending mode is required, the blending temperature is not more than 60 ℃, otherwise, the bacteriostatic ability is easily reduced.

Further, in the step 3), the prepared liquid obtained in the step 1), the sage herb leaf extract and the ginger root extract are added into the solution obtained in the step 2); the ginger root extract accounts for 0.2-2 wt% of the total mass of the natural plant bacteriostatic spray.

Further, in the step 3), adding the prepared liquid obtained in the step 1), the sage herb leaf extract, the moringa seed extract and the biological carbohydrate gum-4 into the solution obtained in the step 2); the moringa seed extract and the biological carbohydrate gum-4 account for 0.2-2 wt% of the total mass of the natural plant bacteriostatic spray.

A natural plant bacteriostatic spray is prepared by the above preparation method.

Compared with the prior art, the invention has the beneficial effects that:

the natural plant bacteriostatic spray provided by the invention does not contain chemical components for disinfection and bacteriostasis, does not contain ethanol, and is mild, safe and non-irritant by adding natural plant components (tiled white bead leaf extract, white willow bark extract, sage herb leaf extract and peony root bark extract) with antibacterial performance and two polyols (pentanediol and 1, 3-propanediol) with bacteriostatic action, reasonable compatibility and mutual synergy, so that the natural plant bacteriostatic spray is safe and efficient for bacteriostasis, does not damage skin barriers and human skins.

Drawings

Fig. 1 is a schematic diagram of a moisture retention capability test of the natural plant formulated bacteriostatic spray of example 1.

Detailed Description

The present invention will be further described with reference to the accompanying drawings and the detailed description, and it should be noted that, in the present invention, various embodiments or technical features described below may be arbitrarily combined to form a new embodiment without conflict.

The components of the natural bacteriostatic spray are described in detail as follows:

1. pentanediol

The method is derived from sugarcane, belongs to a biological carbon source, is environment-friendly and easy to degrade, and adopts an agar dilution method to determine the MIC (minimum inhibitory concentration) value of pentanediol, as shown in the following table:

the pentanediol has excellent bacteriostatic action besides being used as a solvent for the extract of the beautyberry leaves.

2. 1,3 propanediol

Derived from biological glycerol fermentation, passed the raw material certification of ecosert and COSMOS, passed the REACH test, without 168 items of high interest listed by the REACH regulation. Through the patch test, when the concentration of 1, 3-propanediol is as high as 100%, no skin irritation or allergic reaction occurs in 30 subjects. The bacteriostatic performance of 1, 3-propanediol was tested and is shown in the following table:

and (3) judging the effect: the bacteriostasis rate is more than or equal to 50-90%, and the product has bacteriostasis; not less than 90%, has strong bacteriostatic action; is less than 50 percent. According to the table, 1, 3-propylene glycol has strong bacteriostatic action on staphylococcus aureus and escherichia coli.

3. Extract of Paliurus ramosissimus leaf

Also known as wintergreen leaf extract, wintergreen is an evergreen secondary shrub in canada, growing in pines and shrubs in north america. It is used in traditional medicine for treating various diseases (headache and back pain, fever, etc.), and has COSMOS natural certification and skin inflammation relieving effect.

4. White willow bark extract

The bark extracted from white willow is approved for use in the world through ECOCERT authentication, has no limitation, and has good effect of inhibiting microbial activity.

5. Salvia officinalis leaf extract

From Soniam, France, and is listed in International Cosmetic ingredient Dictionary and Handbook. Sage, Sage: a perennial evergreen plant has woody stems, pale gray leaves, and blue to purple flowers. It is a member of the family menthaceae, native to the mediterranean region, and is currently grown in all parts of the world. It has a long history of medicinal and culinary use and is used in modern times as an ornamental landscape plant. In 1753, Carl Linnaeus. For centuries, it has been cultivated for its edible value and healing effect. Numerous documents show that the sage herb leaf extract has the effects of sterilizing and diminishing inflammation.

6. Peony root bark extract

Natural plant antibacterial multi-effect agent, moisturizing multi-effect agent. Deciduous shrubs belonging to Ranunculaceae, without hair, have leaves composed of 1-2 rows, 3 lines and 3-5 leaflets, and are called peony, etc. in summer, and their root bark is called moutan bark, which is dug out in autumn, washed with water, and dried in the sun.

The peony root bark extract is subjected to a sterilization test by the following method:

after the coliform bacteria/staphylococcus/streptococcus are cultured, 0.1% of sterilization and disinfection normal saline of a specimen is added, 0.2% of peony root bark extract is added into a vessel at the temperature of 35 ℃ in the environment, the observation is carried out for 24 hours, and the result is obtained after the reduction of bacteria and the survival number are compared.

The experimental results are as follows:

in the initial stage: coliform colony count 3.7 × 105/pellet colony count 2.2 × 105 pellet colony count 4.3 × 105 saline: 2.2 × 106/2.4 × 106/6.2 × 103 was added to the respective vessels. After 1 hour: the bacteria reduction rate is 50%. After 12 hours: the reduction rate of bacteria is 80%. After 24 hours: the bacteria reduction rate is 100% (reduction rate% (% bacteria total-bacteria rate after killing/bacteria total 100). The peony root bark extract has good antibacterial effect.

7. Arginine

The natural source, ECOCERT certification, is mainly used for pH regulation to dissolve plant bacteriostatic components, so that the plant bacteriostatic components can be completely dissolved in water.

The MIC (minimum inhibitory concentration) of the flat beautyberry leaf extract, the white willow bark extract, the sage herb leaf extract and the peony root bark extract selected in the invention on staphylococcus aureus, escherichia coli, candida albicans, pseudomonas aeruginosa and mould respectively is shown in the following table:

TABLE 1 MIC value antibiogram of plant composition

As can be seen from Table 1, the spreading of the folium Callicarpae Formosanae extract and the white willow bark extract has very low MIC values for Staphylococcus aureus and Escherichia coli, and has very strong inhibitory effect, and the two supplement each other with antibacterial power. The extract of the leaf of the Platanus orientalis and the extract of the leaf of the Salvia officinalis also have low MIC values for Candida albicans, and the combination of the two can inhibit the Candida albicans. The white willow bark extract and the peony root bark extract have low MIC values on pseudomonas aeruginosa, and the white willow bark extract and the peony root bark extract have excellent bacteriostatic ability on the pseudomonas aeruginosa. The white willow bark extract and the sage herb leaf extract have strong inhibition effect on the mould, and the mould belongs to the strains which are very difficult to kill in the strains, but the combination of the white willow bark extract and the sage herb leaf extract can show excellent bacteriostasis capacity at low dosage.

Example 1

A natural plant bacteriostatic spray is composed of the following raw materials in percentage by weight:

1) mixing the white willow bark extract with 1, 3-propylene glycol to obtain 1, 3-propylene glycol solution with concentration of 1% white willow bark extract to obtain a pre-prepared solution;

2) adding arginine into purified water, adjusting pH to 7.0, adding pentanediol solution of folium Callicarpae Formosanae extract, stirring to dissolve completely, and adding EDTA to adjust pH to 5.5;

3) adding the prepared liquid obtained in the step 1) and the sage herb leaf extract into the solution obtained in the step 2), stirring and dissolving, and then adding EDTA to adjust the pH value to 3.5 to obtain the natural plant bacteriostatic spray.

Example 2

1) mixing the white willow bark extract with 1, 3-propylene glycol to obtain 1, 3-propylene glycol solution of white willow bark extract to obtain a pre-prepared solution;

2) adding arginine into purified water, adjusting pH to 6.5, adding a pentanediol solution paved with folium Callicarpae Formosanae extract, stirring to completely dissolve and transparent, and adding EDTA to adjust pH to 3.5;

Example 3

2) adding arginine into purified water, adjusting pH to 7.5, adding a pentanediol solution paved with folium Callicarpae Formosanae extract, stirring to completely dissolve and transparent, and adding EDTA to adjust pH to 6.0;

3) adding the prepared liquid obtained in the step 1) and the sage herb leaf extract into the solution obtained in the step 2), stirring and dissolving, and then adding EDTA to adjust the pH value to 4.0 to obtain the natural plant bacteriostatic spray.

Example 4

2) adding arginine into purified water, adjusting pH to 6.0, adding a pentanediol solution paved with folium Callicarpae Formosanae extract, stirring until the solution is completely dissolved and transparent, and adding EDTA to adjust pH to 4.5;

3) adding the prepared liquid obtained in the step 1), the sage herb leaf extract and the ginger root extract into the solution obtained in the step 2), stirring and dissolving, and then adding EDTA to adjust the pH value to 4.0 to obtain the natural plant bacteriostatic spray.

Example 5

2) adding arginine into purified water, adjusting pH to 6.0, adding a pentanediol solution paved with folium Callicarpae Formosanae extract, stirring until the solution is completely dissolved and transparent, and adding EDTA to adjust pH to 5.0;

3) adding the prepared liquid obtained in the step 1), phytic acid, the sage herb leaf extract, the moringa seed extract and biological carbohydrate gum-4 into the solution obtained in the step 2), stirring and dissolving, and then adding EDTA (ethylene diamine tetraacetic acid) to adjust the pH value to 4.0 to obtain the natural plant bacteriostatic spray.

Comparative example 1

Comparative example 1 in comparison with example 1, the extract of the kombucha leaf was absent, and the remaining components and preparation method were the same as in example 1.

Comparative example 2

Comparative example 2 compared to example 1, the sage extract was absent and the remaining components were prepared in the same manner as in example 1.

Comparative example 3

Comparative example 3 compared with example 1, the peony root bark extract is absent, and the rest components and the preparation method are the same as example 1.

Comparative example 4

Comparative example 4 in comparison with example 1, the white willow bark extract was absent and the remaining components were prepared in the same manner as in example 1.

Performance testing

Firstly, the spray of example 1 and comparative examples 1-4 are subjected to bacteriostasis test of staphylococcus aureus

According to the data in the table, the bacteriostatic spray of the natural plant in the example 1 can reach a bacteriostatic rate of more than 99.98% within 2min, which indicates that the product has a rapid and powerful bacteriostatic action. And when the sprays of comparative examples 1-4 are used for 2min, the bacteriostasis rate is lower than that of example 1. The four plant extracts are proved to play a synergistic bacteriostatic role.

Second, the spray of example 1 was tested for moisturizing ability

The German CK skin moisture tester is used for testing the skin moisture content between 0-4 h of the arm using the spray of the example 1 and the arm without spraying, and as shown in figure 1, the result shows that the spray of the example 1 has stronger moisturizing capability and does not damage the skin barrier after being used.

The above embodiments are only preferred embodiments of the present invention, and the protection scope of the present invention is not limited thereby, and any insubstantial changes and substitutions made by those skilled in the art based on the present invention are within the protection scope of the present invention.

Claims

1. A preparation method of a natural plant bacteriostatic spray is characterized by comprising the following steps:

2) adding arginine into purified water, adjusting the pH value to 6.5-7.5, adding a pentanediol solution of the beautyberry leaf extract, stirring until the extract is completely dissolved, and adjusting the pH value to 3.5-6.0;

3) adding the prefabricated liquid obtained in the step 1) and the sage herb leaf extract into the solution obtained in the step 2), stirring and dissolving, and then adjusting the pH value to 3.5-4.0 to obtain a natural plant bacteriostatic spray;

2. The method for preparing bacteriostatic spray according to claim 1, wherein the concentration of the white willow bark extract in the 1, 3-propanediol solution of the white willow bark extract in step 1) is 1%.

3. The method for preparing the natural plant bacteriostatic spray according to claim 1, wherein in the step 2), EDTA is used to adjust the pH to 3.5-6.0.

4. The method for preparing the natural plant bacteriostatic spray according to claim 1, wherein in the step 3), EDTA is used to adjust the pH to 3.5-4.0.

5. The preparation method of the natural plant bacteriostatic spray according to claim 1, characterized in that in the steps 1) to 3), the reaction temperature is not more than 60 ℃.

6. The method for preparing a natural plant bacteriostatic spray according to claim 1, wherein in the step 3), the prepared solution obtained in the step 1), the sage herb leaf extract and the ginger root extract are added into the solution obtained in the step 2); the ginger root extract accounts for 0.2-2 wt% of the total mass of the natural plant bacteriostatic spray.

7. The method for preparing the natural plant bacteriostatic spray according to claim 1, wherein in the step 3), the prepared liquid obtained in the step 1), the sage herb leaf extract, the moringa seed extract and the biogum-4 are added into the solution obtained in the step 2); the moringa seed extract and the biological carbohydrate gum-4 account for 0.2-2 wt% of the total mass of the natural plant bacteriostatic spray.

8. A natural plant bacteriostatic spray which is prepared by the preparation method according to any one of claims 1 to 7.