CN101671204A - Green environment protective biological organic compound fertilizer and production method thereof - Google Patents

Green environment protective biological organic compound fertilizer and production method thereof Download PDF

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Publication number
CN101671204A
CN101671204A CN200910181133A CN200910181133A CN101671204A CN 101671204 A CN101671204 A CN 101671204A CN 200910181133 A CN200910181133 A CN 200910181133A CN 200910181133 A CN200910181133 A CN 200910181133A CN 101671204 A CN101671204 A CN 101671204A
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fertilizer
gram
bacterial
organic
substratum
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李春龙
李健
赵文涛
魏立新
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BEIJING ZHONGRUI HUATAI APPLIED TECHNOLOGY RESEARCH INSTITUTE
BEIJING SENIOR EXPERT TECHNOLOGY CENTER CHINESE ACADEMY OF SCIENCES
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BEIJING ZHONGRUI HUATAI APPLIED TECHNOLOGY RESEARCH INSTITUTE
BEIJING SENIOR EXPERT TECHNOLOGY CENTER CHINESE ACADEMY OF SCIENCES
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Priority to CN200910181133A priority Critical patent/CN101671204A/en
Publication of CN101671204A publication Critical patent/CN101671204A/en
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/20Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses

Abstract

The invention relates to a green environment protective biological organic compound fertilizer produced by adopting biological azotobacter, organic phosphorus bacteria, crop growth factor, organism, inorganic substance and microelement which are processed by a special technology, and a production method thereof. Biological bacterial fertilizer, organic fertilizer and inorganic fertilizer are interdependent and decomposed, and play mutual synergistic action to reach the special efficacy of fully utilizing the biological bacterial fertilizer to decompose the organic fertilizer and the inorganicfertilizer and reach the effect of stimulating the growth of crops, thus leading crops to absorb and utilize fertilizer well so as to achieve the purposes of crop production increasing and fertilizersaving. The invention has simple technical method and process, effectively utilizes urban garbage and subsidiary agricultural product offal as raw materials of the organic fertilizer, saves inorganicfertilizer, reduces cost, can be widely used in crops and fruit bearing forests, is especially suitable for greenhouses, base fertilizer and additional fertilizer of crops at the temperature of more than 20 DEG C, and is suitable for popularization and application across the country.

Description

Green environment protective biological organic compound fertilizer and production method thereof
Technical field
The present invention relates to a kind of green environment protective biological organic compound fertilizer and production method thereof, belong to a kind of employing biological nitrogen fixation bacterium, phosphorus bacteria, potassium bacterium and organic raw material, inorganic raw material, trace element cooperation, the short long factor of preferred best compatibility and making or interpolation farm crop is made the high-performance bio compounded granular fertilizer through novel process.
Technical background
Green environment protective biological organic compound fertilizer and production method thereof are to draw the elite of traditional organic fertilizer, in conjunction with modern biotechnology, and the high-tech product that processes.Its nutritive element collection is quick-acting, long-acting, synergy is one, has the quality of agricultural product of raising, suppresses soil-borne disease, strengthens crop anti-adversity, promotes the effect of crop precocious.We obtain the high reactivity bacterial classification through separation, purifying, mutagenesis and make organic potassium, phosphorus bacteria and actinomycetes plant stimulin, produce the nuisanceless green biological organic composite fertilizer with various trace elements through special process, effectively utilized municipal wastes, agricultural byproducts tankage and animal excrement organic matter material as bio-feritlizer, be that refuse has obtained effectively utilizing, turn waste into wealth, also reduced environmental pollution simultaneously.The microbial inoculum that is comprised has the organic substance of acceleration Decomposition, makes or transforms available nutrient for crop " power " is provided.Microbial inoculum is with the effect that improves potential nutrient in chemical fertilizer utilization ratio and the activating soil simultaneously.Wherein cooperate a spot of chemical fertilizer, regulation of fertilizer requirement and fertilizer characteristic according to farm crop are carried out scientific matching, with biological " activator " perfect combination, except that containing middle trace element such as nitrogen, phosphorus, potassium macronutrient and calcium, magnesium, sulphur, iron, boron, zinc, selenium, molybdenum, also contain the fertile synergistic agent of large amount of organic matter, humic acid material and guarantor, nutrient is complete, the slow Ji mutually of speed, for fertile balanced, fertilizer efficiency is lasting.
This technology adopts organic and inorganic, biological Trinitarian cooperation, be suitable for using of all kinds of farm crop and vegetables base fertilizer thereof, can be widely used in green agriculture, the ecological agriculture and environmental protection agricultural fertilizer, on the make adopt granule, make it this fertilizer and have long-lasting preferably.
Have many pieces of patent documents to disclose all production methods of utilizing bio-bacterial manure to produce compound manure in the prior art, wherein immediate documents is:
Publication number CN1403418A discloses and has utilized bio-bacterial manure, cooperates with inorganic raw material to produce a kind of organic-biological compound fertilizer, and its shortcoming is: the one, and bacterial classification and bacterial manure are made not open; The 2nd, select for use inorganic raw material single;
Publication number CN1654443A discloses the biological bacterium compound manure, cooperates with inorganic raw material to produce a kind of organic-biological compound fertilizer, and its shortcoming is: the bio-bacterial manure manufacturing does not have full disclosure, is difficult to realize in producing and making;
Publication number CN1951875A discloses and has utilized bio-bacterial manure to cooperate a kind of organic-biological compound fertilizer of production, and its shortcoming is: the one, and the title of bio-bacterial manure and numbering and technology do not have full disclosure, are difficult to make bio-bacterial manure.
Summary of the invention
It is not enough to the objective of the invention is to overcome prior art processes design and prescription, a kind of green environment protective biological organic compound fertilizer and production method thereof are provided, this method adopts with cooperations such as organic raw material, bio-bacterial manure, inorganicss, particularly utilize municipal wastes organic matter, agricultural byproducts offal and various cake, animal byproducts (skin, hair, bone) etc., can effectively realize the comprehensive utilization of refuse, turn waste into wealth.
The green environment protective biological organic compound fertilizer and the production method thereof of present technique invention comprise following step:
1. the preparation of raw material comprises mutagenesis, separation, the purifying of bacterial classification; The making of microbial inoculum and bacterial manure substratum and fermentation; The preparation of fertilizer and making; The preparation of inorganic fertilizer and prescription; The preparation and the making of trace element; The prescription of composite fertilizer and technology are:
(1) mutagenesis of bacterial classification, separation, purifying;
(2) making and the zymotechnique of bacteria agent and bacterial manure substratum;
(3) substratum of bio-bacterial manure, sterilization, inoculation, fermentation;
(4) preparation of fertilizer and making comprise humic acid fertilizer and fertile two classes of organic matter material;
(5) preparation of inorganic fertilizer and prescription;
(6) making of organic matter fermentation agent;
(7) trace element is allocated into;
(8) prescription of green environment protective biological organic compound fertilizer and preparation.
2. wherein prescription and technology are:
(1) the trace element amount of allocating into (mass parts) is: 2-6 part.Its cooperation ratio is:
1. sal epsom 0.5-2.4,2. lime carbonate 0.5-2.4,3. boric acid 0.05-0.16,4. zinc sulfate 0.15-0.22,5. manganous sulfate 0.25-0.32,6. ammonium molybdate 0.005-0.012,7. copper sulfate 0.5-0.12,8. Xanthohumate 0.15-0.22,9. rare earth 0.04-0.092;
(2) prescription of high-performance bio compounded granular fertilizer and preparation:
AS4.891Steptomyces miroftauus streptomyces microflavus and AS1.910 BaciLLus muciLaginosus bacillusmusilaginosiengineering bio-bacterial manure 30-60%
(3) amount of allocating into of inorganic raw material is: 30-70
Nitrogen, phosphorus, potassium content proportioning are: 3-10: 2-6: 1-4
Urea content is 46.6%, adds (mass parts) to be: 5-20 part;
Phosphorus content is 14%, and add-on (mass parts) is 20-60;
KCE content is 58%, and add-on (mass parts) is: 6-16 part;
(4) the fertilizer amount of allocating into (mass parts) is: 30-70
(5) humic acid fertilizer 15-20
(6) the fertile 15-40 of organic matter material
(7) the catalytic organism agent is made
Prescription A:
1. rice bran 16%
2. oil meal (slag) 13%
3. dregs of beans 14%
4. sugar 7%
5. water 50%
By above formula rate, earlier glucide is added in the water, after the stirring and dissolving, add rice bran, oil meal (slag) and dregs of beans, after fully mixing, stack, in about 60 ℃, keep fermentation in 20~40 days, 40 orders were pulverized in oven dry;
Prescription B:
1. the A finished product 48% of filling a prescription
2. anhydrous slufuric acid ferrous 48%
3. citric acid 4%
Mix by above proportioning, promptly become the organic fertilizer catalyzer.
(8) make mixing granulation:
Elder generation is with fertilizer and humic acid phosphate fertilizer adds in the mixing machine or adding flyash; adding bio-bacterial manure again stirs; thereafter adding inorganics mixes; the composite fertilizer's batch mixing that mixes is arrived the tablets press granulation by conveyor delivery; air stream drying or oven drying at low temperature promptly become the complete first compounded granular fertilizer of high-effect organic biological:
In the present invention, biological composite bacterial manure adopts agricultural byproducts offal or excrement of animals or the municipal wastes organism substratum as bio-bacterial manure, and add of the short long agent of a spot of phosphate fertilizer as biology, preferably resolve the growth processing environment pollution problem preferably again that to satisfy biological bacteria, make bio-bacterial manure in compound manure, play synergy.Wherein, bacillusmusilaginosiengineering bacterial fertilizer, it can decompose the potassium in the mineral out, can obtain the plain nutrition of phosphorus again from hard phosphorus ash, can also be from air fixing nitrogen, can strengthen the crop disease-resistant ability simultaneously; Streptomyces microflavus can produce and irritate element and antibiotic, farm crop is had diseases prevention, keeps a full stand of seedings, loosens the soil and irritate multiple effects such as plant growth; Mineral manure has quick-acting to farm crop, and they participate in the vital movement of crop in every way, plays a part constituent and metabolic activities compound in the vegetable cell; AS1.867Pseudomonas fturescens Pseudomonas fluorescens bacterial manure, the inorganic phosphorus that can decompose invalid attitude is an available phosphorus; Trace element has single-minded physiological action in plant-growth, they interdepend in the whole growth process of plant, condition each other, and are in a kind of equilibrium state; Fertilizer can make full use of agricultural and sideline product offal and municipal wastes organism, reduces fertilizer cost, and can be the carrier that the existence of biological bacteria in compound manure is played protection and nutrition is provided.
3. high-performance bio compounded granular fertilizer of the present invention and production method thereof have the following advantages and effect:
(1) adopts farm crop by product, municipal wastes organic matter, animal skin offal, increased the organic matter and the proteic content in soil, reduced hardening of soil, for the growth of bio-bacterial manure provides good cultivation material and nutriment;
(2) adopt phosphorus decomposing, potassium bacterium fertilizer, the absorption that has improved organic and inorganics phosphorus transforms, and has played mutual synergy;
The cooperation of (3) employing bio-bacterial manure, organic matter, mineral manure, trace element has improved fertilizer efficiency, has reduced the utilization of inorganic fertilizer, has saved raw material, has reduced cost;
(4) effectively utilize farm crop by product and animal excrement and municipal wastes organic matter, refuse is turned waste into wealth, simultaneously, improved environment, its economic benefit and social benefit are quite remarkable;
(5) be rich in biologically active substance and somatomedin, farm crop had the effect that stimulates growth, preventing disease and pest.
Every technical indicator of present technique invention design meets relative national standards, through field experiment, has all obtained good effect.
The high-performance bio compounded granular fertilizer that the by product of present technique design employing municipal wastes organism, crop products, animal excrement, inorganics, trace element etc. design and produce, compare with inorganic fertilizer, price has reduced 15-30%, and fertilizer efficiency has improved 10-20%.
Used following bio-bacterial manure and organic, micro-in the present technique invention, wherein, biological inoculum is to buy from China Committee for Culture Collection of Microorganisms common micro-organisms center and behind resurrection, mutagenesis, separation, purifying, chooses the high reactivity bacterial strain as producing bacterial classification; Mineral manure nitrogen, phosphorus, potassium and trace element need to buy from market; The organic need collected or purchased from relevant department, handles through fermentation and oven dry.
4. the present technique invention comprises mutagenesis, separation, the purifying of bacterial classification; The making of microbial inoculum and bacterial manure substratum and fermentation; The preparation of fertilizer and making; The preparation of inorganic fertilizer and prescription; The preparation and the making of trace element; The catalytic organism agent is made; The prescription of composite fertilizer and technology:
1) bacterial classification of biological composite bacterial manure is:
AS1.910 BaciLLus muciLaginosus bacillusmusilaginosiengineering; (2) AS4.891Steptomycesmiroftauus streptomyces microflavus.
2) mutagenesis of bacterial classification, separation, purifying are:
(1) preparation bouillon media;
(2) bouillon media was sterilized 30 minutes in 1.5 kg/cm;
(3) under aseptic technique, get the above bacterial classification of a ring and put into bouillon media;
(4) micro oscillation juice substratum, the streptomyces antibioticus of adding trace makes it and spares;
(5) will pour in the culture dish with the substratum of even sample to be separated, shake up;
(6) uviolizing is handled and purebred cultivation.
Opened the UV-lamp preheating earlier 15 minutes, make light wave stable, then culture dish is inserted darkroom or sterilisable chamber, from about UV-lamp 15-25 centimetre, locating to shine 1-3 minute (deciding) on the active different of bacterial classification, open red light or other colored lights simultaneously, after irradiation finishes, culture dish wrapped up the culture dish of handling with black cloth or other dark cloth insert in the incubator, under 28-30 ℃ of constant temperature, cultivated 24-72 hour, it is vigorous to select growth, active high bacterial strain inserts in the test tube slant substratum to be cultivated, mutagenesis so repeatedly, separate, screening, there is not assorted bacterium until from test tube or culture dish, filtering out, energetic effective bacterial classification inserts in the slant tube then as producing bacterial classification or making the vacuum lyophilization peace bottle normal phase of pipe and preserve.
3) making of microbial inoculum substratum and fermentation:
(1) making of AS1.910 BaciLLus muciLaginosus bacillusmusilaginosiengineering kind microbial inoculum:
1. the making of slant strains: potassium primary phosphate kh 2Po 40.02-0.04 gram, dipotassium hydrogen phosphate k 2HpO 40.04-0.10 gram, sal epsom MgSO 4.7h 2The o0.01-0.02 gram, calcium sulfate Ca 4.SO 42h 2The o0.005-0.012 gram, iron(ic) chloride feCl 3Trace, molybdic acid are received Na 2MoO 4.2h 2The o trace, yeast extract 0.05 gram, N.F,USP MANNITOL 2 grams, agar 1.5 grams, 100 milliliters of distilled water, ph value 7.2;
2. second class inoculum solid medium (mass parts) is: potassium primary phosphate kh 2Po 40.01-0.02 gram, dipotassium hydrogen phosphate k 2HpO 40.04-0.10 gram, sal epsom MgSO 4.7h 2The o0.01-0.02 gram, calcium sulfate CaSO 4.2h 2The o0.005-0.015 gram, rice bran (wheat bran or cotton seed cake) 20-60, crop stalk powder 20-40, loam 20-40, calcium superphosphate 0.5-1.5 part, the peat composed of rotten mosses or plant ash or flyash 3-6 regulate about ph value 7-7.5 with unslaked lime;
Medium sterilization, inoculation and cultural method are:
Medium sterilization: above slant medium, secondary microbial inoculum solid medium culture material are mixed, slant medium is packed in vitro, all the other secondary mediums are put into Cans or triangular flask or cloth bag, oral area or top are with cotton or multilayer gauze or kraft paper gag, substratum is put into or is inserted Autoclave, with 1.5 kg/cm pressure sterilizations 30 minutes;
Culture medium inoculated and cultural method are: when treating that substratum cools to 40 ℃ of following temperature, insert female bacterial classification or activation peace bottle dry freeze pipe bacterial classification by aseptic technique test tube slant substratum, secondary medium inserts inclined-plane or triangular flask bacterial classification, temperature is controlled at about 25-28 ℃, fermentation time generally was controlled at 48-72 hour, and microbial inoculum can be as the production microbial inoculum of bacterial manure after microscopy is qualified.
(2) making of AS4.891Steptomyces miroftauus streptomyces microflavus bacterial manure:
1. the making of slant strains: Zulkovsky starch 2 grams, lime carbonate KNO 31 gram, dipotassium hydrogen phosphate k 2HpO 40.05 gram, sodium-chlor NaCI0.05 gram, ferric sulfate FeSO 4.7H 2The O0.001 gram, agar 1.5 grams, 100 milliliters of distilled water, ph value 7.2-7.4;
2. dipotassium hydrogen phosphate k 2HpO 40.05 gram, sal epsom MgSO 4.7H 2The O0.05 gram, lime carbonate KNO 30.5 gram, soybean cake powder 20-40 part, loam 50-80 part, straw powder 25-50, calcium superphosphate 0.5-1.5 part, the peat composed of rotten mosses or plant ash or flyash 3-6,
Regulate about ph value 7-7.5 with unslaked lime; Medium sterilization, inoculation and cultural method are:
Medium sterilization: above slant medium, secondary microbial inoculum solid medium culture material are mixed, slant medium is packed in vitro, all the other secondary mediums are put into Cans or triangular flask inner bag or cloth, oral area or top are with cotton or multilayer gauze or kraft paper gag, substratum is put into or is inserted Autoclave, with 1.5 kg/cm pressure sterilizations 30 minutes;
Culture medium inoculated and cultural method are: when treating that substratum cools to 40 ℃ of following temperature, insert female bacterial classification or activation peace bottle dry freeze pipe bacterial classification by aseptic technique test tube slant substratum, secondary medium inserts inclined-plane or triangular flask bacterial classification, temperature is controlled at about 25-28 ℃, fermentation time generally was controlled at 48-72 hour, and microbial inoculum can be as the production microbial inoculum of bacterial manure after microscopy is qualified.
4) making of bacterial manure and substratum and fermentation:
Wherein the compatibility of bacterial manure is:
AS4.891Steptomyces miroftauus streptomyces microflavus and AS1.910 BaciLLus muciLaginosus bacillusmusilaginosiengineering:
Substratum, sterilization, inoculation, the fermentation of the bio-bacterial manure of AS4.891Steptomyces miroftauus streptomyces microflavus are:
Dipotassium hydrogen phosphate k 2HpO 40.01-0.05 part, potassium primary phosphate kh 2Po 40.01-0.02 part, sal epsom MgSO 4.7H 2O0.01-0.05 part, soya-bean cake 2-5 part, crop stalk powder 3-8 part, wheat bran 2-5 part, calcium superphosphate 0.5-1.5 part, flyash or peat composed of rotten mosses 5-10 part, loam or organic 50-73 part, adjusting the ph value with lime is 7.5-8, and humidity is adjusted to about 30-40%, normal-pressure sterilization 2-3 hour or autoclaving 30 minutes, when treating that material cools to the 40 degree left and right sides, insert 2 grades of microbial inoculums, the access amount is 1-10%, insert after stirring in fermentation machine or fermentation frame or fermentation vat or the fermentation dish, shallow-layer (20 centimetres) or deep ventilation fermentation about control material temperature 30 degree, are not stirred, cultivated 3-5 days, and promptly can be high-quality bacterial manure;
Substratum, sterilization, inoculation, the fermentation of AS1.910 BaciLLus muciLaginosus bacillusmusilaginosiengineering bacterial manure are:
Potassium primary phosphate kh 2Po 40.01-0.02 part, dipotassium hydrogen phosphate k 2HpO 40.04-0.02 gram, sal epsom Mgso 4.7h 2O0.01-0.02 part, 5 parts of soya-bean cakes, 8 parts in crop stalk powder, 5 parts in wheat bran, 1 part of calcium superphosphate, 8 parts of the flyash or the peats composed of rotten mosses, 73 parts of loam or organic matters, adjusting the ph value with lime is 7.5-8, and humidity is adjusted to about 30-40%, normal-pressure sterilization 2-3 hour or autoclaving 30 minutes, when treating that material cools to the 40 degree left and right sides, insert 2 grades of microbial inoculums, the access amount is 1-10%, insert after stirring in fermentation machine or fermentation frame or fermentation vat or the fermentation dish, shallow-layer (20 centimetres) or deep ventilation fermentation about control material temperature 30 degree, are not stirred, cultivated 3-5 days, and promptly can be high-quality bacterial manure;
5) preparation of fertilizer and making:
(1) humic acid fertilizer is prepared and is made:
1. weathered coal or peat or peat composed of rotten mosses 60-100 part are ready to;
2. take by weighing 20 parts of dilute sulphuric acid proportions about 1.28, mix with an amount of water;
3. weathered coal or peat or the peat composed of rotten mosses are mixed with the mixture of dilute sulphuric acid and water, stir acidification 24-48 hour;
4. the weathered coal after the acidification or peat or the peat composed of rotten mosses and 15-30 part ground phosphate rock or phosphate fertilizer mixing and stirring, reaction 5-6 days of banking up is humic-acid kind phosphate fertilizer.
(2) the catalytic organism agent is made
Prescription A:
1. rice bran 16%
2. oil meal (slag) 13%
3. dregs of beans 14%
4. sugar 7%
5. water 50%
By above formula rate, earlier glucide is added in the water, after the stirring and dissolving, add rice bran, oil meal (slag) and dregs of beans, after fully mixing, stack, in about 60 ℃, keep fermentation in 20~40 days, 40 orders were pulverized in oven dry;
Prescription B:
1. the A finished product 48% of filling a prescription
2. anhydrous slufuric acid ferrous 48%
3. citric acid 4%
Mix by above proportioning, promptly become the organic fertilizer catalyzer.
(3) pre-treatment of organic matter material (bag Chinese juniper municipal wastes or animal excrement and fur offal or agricultural byproducts offal and various biscuit):
1. the residue of municipal wastes under residue after the magnetic separation stage treatment behind the adding catalyzer, through banking up more than spontaneous fermentation 8-15 days, passes through 10-30 mesh sieve again, and filtrate is planted matter for organic and corruption, is good organic fertilizer after drying or drying;
2. add part grass meal or saw foam or weathered coal soil in the animal excrement, add catalyzer again through spontaneous fermentation in 5-10 days or add biological organophosphorus bacterial fertilizer three-class strain fermentation 3-5 days, be good organic fertilizer after drying or drying;
3. the animal skin offal was pulverized 10-30 mesh sieve, added catalyzer earlier, added bio-azotobacter fertilizer material microbial inoculum and organophosphorus bacterial fertilizer or secondary bacteria fermentation again 4-6 days, was good organic protein fertilizer after drying or drying.
(4) amount of allocating into of inorganic raw material is: 30-70
1. nitrogen, phosphorus, potassium content proportioning are: 3-10: 2-6: 1-4
2. urea content is 46.6%, adds (mass parts) to be: 5-20 part;
3. phosphorus content is 14%, and add-on (mass parts) is 20-60;
4. KCE content is 58%, and add-on (mass parts) is: 6-16 part;
(5) Wei Liangyuansu preparation and making:
The trace element amount of allocating into (mass parts) is: 2-6 part.Its cooperation ratio is:
1. sal epsom 0.5-2.4,2. lime carbonate 0.5-2.4,3. boric acid 0.05-0.16,4. zinc sulfate 0.15-0.22,5. manganous sulfate 0.25-0.32,6. ammonium molybdate 0.005-0.012,7. copper sulfate 0.5-0.12,8. Xanthohumate 0.15-0.22,9. rare earth 0.04-0.092;
5. the prescription of composite fertilizer and technology:
1) the bacterial manure amount of allocating into AS4.891Steptomyces miroftauus streptomyces microflavus and bacillusmusilaginosiengineering AS1.910BaciLLus muciLaginosus (mass parts) are: 10-60
2) amount of allocating into of inorganic raw material (mass parts) is: 30-60
3) nitrogen, phosphorus, potassium content proportioning are: 3-8: 2-6: 1-5
1. urea content is 46.6%, adds (mass parts) to be: 5-19 part;
2. phosphorus content is 14%, and add-on (mass parts) is 20-50;
3. KCE content is 58%, and add-on (mass parts) is: 6-14 part.
4) the fertilizer amount of allocating into (mass parts) is: 30-60
1. humic acid fertilizer 15-30
2. the fertile 15-30 of organic matter material
5) the trace element amount of allocating into (mass parts) is: 2-8 part, and its cooperation ratio is:
6. high-performance bio compounded granular fertilizer example
Example 1
(1) compatibility of bio-bacterial manure and the amount of allocating into are:
AS4.891Steptomyces miroftauus streptomyces microflavus bacillusmusilaginosiengineering AS1.910 BaciLLusmuciLaginosus 44 (parts):
(2) amount of allocating into of inorganic raw material (mass parts) is 25
1. urea content is 46.6%, adds (mass parts) to be: 6;
2. phosphorus content is 14%, and add-on (mass parts) is 15;
3. KCE content is 58%, and add-on (mass parts) is: 4
(3) the fertilizer amount of allocating into (mass parts) is: 27
1. the humic acid fertilizer amount of allocating into (mass parts) is: 10
2. the organic matter material fertilizer amount of allocating into (mass parts) is: 17
(4) the trace element amount of allocating into (mass parts) is: 4 parts: its cooperation ratio is:
1. sal epsom 2, and 2. lime carbonate 2, and 3. boric acid 0.1, and 4. zinc sulfate 0.2, and 5. manganous sulfate 0.3, and 6. ammonium molybdate 0.01, and 7. copper sulfate 0.8, and 8. Xanthohumate 0.02, and 9. rare earth 0.1;
Example 2
(1) compatibility of bio-bacterial manure and the amount of allocating into are 10%
AS1.910 BaciLLus muciLaginosus bacillusmusilaginosiengineering and AS4.891Steptomyces miroftauus streptomyces microflavus 10%
(2) amount of allocating into of inorganic raw material (mass parts) is: 30
1. urea content is 46.6%, adds (mass parts) to be: 12;
2. phosphorus content is 14%, and add-on (mass parts) is 12;
3. KCE content is 58%, and add-on (mass parts) is: 6
(3) the fertilizer amount of allocating into (mass parts) is: 56
1. the humic acid fertilizer amount of allocating into (mass parts) is: 16
2. the organic matter material fertilizer amount of allocating into (mass parts) is: 40
(4) the trace element amount of allocating into (mass parts) is: 4 parts: its cooperation ratio is:
1. sal epsom 2, and 2. lime carbonate 2, and 3. boric acid 0.1, and 4. zinc sulfate 0.2, and 5. manganous sulfate 0.3, and 6. ammonium molybdate 0.01, and 7. copper sulfate 0.8, and 8. Xanthohumate 0.02, and 9. rare earth 0.1;
7. mix and granulation
Make mixing granulation:
Elder generation is with fertilizer and humic acid phosphate fertilizer adds in the mixing machine or adding flyash, adds bio-bacterial manure again and stirs, and adds inorganics thereafter and mixes, and the composite fertilizer's batch mixing that mixes is arrived the tablets press granulation by conveyor delivery; Be total to granulation perhaps earlier inorganic fertilizer and fertilizer elder generation mixing granulation, and then with bio-bacterial manure.Air stream drying or oven drying at low temperature promptly become the high-performance bio compounded granular fertilizer.

Claims (5)

1. green environment protective biological organic compound fertilizer and production method thereof comprise mutagenesis, separation, the purifying of bacterial classification; The making of bacterial classification and microbial inoculum substratum and fermentation; The making of bacterial manure substratum and fermentation; The preparation of fertilizer and making; The preparation of inorganic fertilizer and prescription; The preparation and the making of trace element; The prescription of composite fertilizer and technology;
Wherein the bacterial classification of biological composite bacterial manure is:
(1) AS1.910 Bacillus mucilaginous bacillusmusilaginosiengineering;
(2) AS4.891Steptomyces miroftauus streptomyces microflavus.
2. a kind of green environment protective biological organic compound fertilizer according to claim 1 and production method thereof is characterized in that mutagenesis, separation and the purifying of above-mentioned bacterial classification:
(1) preparation bouillon media;
(2) bouillon media was sterilized 30 minutes in 1.5 kg/cm;
(3) under aseptic technique, get the above bacterial classification of a ring and put into bouillon media;
(4) micro oscillation bouillon media, the streptomyces antibioticus of adding trace makes it and spares;
(5) will pour in the culture dish with the substratum of even sample to be separated, shake up;
(6) uviolizing is handled and purebred cultivation;
Opened the UV-lamp preheating earlier 15 minutes, make light wave stable, then culture dish is inserted darkroom or sterilisable chamber, from about UV-lamp 15-25 centimetre, locating to shine 1-3 minute (deciding) on the active different of bacterial classification, open red light or other colored lights simultaneously, after irradiation finishes, culture dish wrapped up the culture dish of handling with black cloth or other dark cloth insert in the incubator, under 28-30 ℃ of constant temperature, cultivated 24-72 hour, it is vigorous to select growth, active high bacterial strain inserts in the test tube slant substratum to be cultivated, mutagenesis so repeatedly, separate, screening, there is not assorted bacterium until from test tube or culture dish, filtering out, energetic effective bacterial classification inserts in the slant tube then as producing bacterial classification or making the vacuum lyophilization peace bottle normal phase of pipe and preserve.
3. a kind of green environment protective biological organic compound fertilizer according to claim 1 and production method thereof is characterized in that the making of above-mentioned biological inoculum and microbial inoculum substratum and zymotechnique are:
(1) making of AS4.891Steptomyces miroftauus streptomyces microflavus bacterial manure:
1. the making of slant strains: Zulkovsky starch 2 grams, lime carbonate KNO 31 gram, dipotassium hydrogen phosphate k 2HpO 40.05 gram, sodium-chlor NaCI0.05 gram, ferric sulfate FeSO 4.7H 2The O0.001 gram, agar 1.5 grams, 100 milliliters of distilled water, ph value 7.2-7.4;
2. dipotassium hydrogen phosphate k 2HpO 40.05 gram, sal epsom MgSO 4.7H 2The O0.05 gram, lime carbonate KNO 30.5 gram, soybean cake powder 20-40 part, loam 50-80 part, straw powder 25-50, calcium superphosphate 0.5-1.5 part, the peat composed of rotten mosses or plant ash or flyash 3-6 regulate about ph value 7-7.5 with unslaked lime; Medium sterilization, inoculation and cultural method are:
Medium sterilization: above slant medium, secondary microbial inoculum solid medium culture material are mixed, slant medium is packed in vitro, all the other secondary mediums are put into Cans or triangular flask or cloth bag, oral area or top are with cotton or multilayer gauze or kraft paper gag, substratum is put into or is inserted Autoclave, with 1.5 kg/cm pressure sterilizations 30 minutes;
Culture medium inoculated and cultural method are: when treating that substratum cools to 40 ℃ of following temperature, insert female bacterial classification or activation peace bottle dry freeze pipe bacterial classification by aseptic technique test tube slant substratum, secondary medium inserts inclined-plane or triangular flask bacterial classification, temperature is controlled at about 25-28 ℃, fermentation time generally was controlled at 48-72 hour, and microbial inoculum promptly can be used as the production microbial inoculum of bacterial manure after microscopy is qualified;
(2) making of AS1.910BaciLLus muciLaginosus bacillusmusilaginosiengineering kind microbial inoculum:
1. the making of slant strains: potassium primary phosphate kh 2Po 40.02-0.04 gram, dipotassium hydrogen phosphate k 2HpO 40.04-0.10 gram, sal epsom MgSO 4.7h 2The o0.01`-0.02 gram, calcium sulfate Ca 4.SO 42h 2The o0.005-0.012 gram, iron(ic) chloride feCl 3Trace, molybdic acid are received Na 2MoO 4.2h 2The o trace, yeast extract 0.05 gram, N.F,USP MANNITOL 2 grams, agar 1.5 grams, 100 milliliters of distilled water, ph value 7.2;
2. second class inoculum solid medium (mass parts) is: potassium primary phosphate kh 2Po 40.01-0.02 gram, dipotassium hydrogen phosphate k 2HpO 40.04-0.10 gram, sal epsom MgSO 4.7h 2The o0.01-0.02 gram, calcium sulfate CaSO 4.2h 2The o0.005-0.015 gram, rice bran (wheat bran or cotton seed cake) 20-60, crop stalk powder 2040, loam 2040, calcium superphosphate 0.5-1.5 part, the peat composed of rotten mosses or plant ash or flyash 3-6 regulate about ph value 7-7.5 with unslaked lime;
Medium sterilization, inoculation and cultural method are:
Medium sterilization: above slant medium, secondary microbial inoculum solid medium culture material are mixed, slant medium is packed in vitro, all the other secondary mediums are put into Cans or triangular flask or cloth bag, oral area or top are with cotton or multilayer gauze or kraft paper gag, substratum is put into or is inserted Autoclave, with 1.5 kg/cm pressure sterilizations 30 minutes;
Culture medium inoculated and cultural method are: when treating that substratum cools to 40 ℃ of following temperature, insert female bacterial classification or activation peace bottle dry freeze pipe bacterial classification by aseptic technique test tube slant substratum, secondary medium inserts inclined-plane or triangular flask bacterial classification, temperature is controlled at about 25-28 ℃, fermentation time generally was controlled at 48-72 hour, and microbial inoculum can be as the production microbial inoculum of bacterial manure after microscopy is qualified.
4. biological compounded granular fertilizer of a kind of Nuisanceless high-efficient according to claim 1 and production method thereof is characterized in that the preparation and the making of above-mentioned fertilizer:
Organic fertilizer comprises two classes:
(1) humic acid fertilizer is prepared and is made:
1. weathered coal or peat or peat composed of rotten mosses 60-100 part are ready to;
2. take by weighing 10-30 part dilute sulphuric acid proportion about 1.28, mix with an amount of water;
3. weathered coal or peat or the peat composed of rotten mosses are mixed with the mixture of dilute sulphuric acid and water, stir acidification 24-72 hour;
4. weathered coal or peat or the peat composed of rotten mosses and 15-30 part ground phosphate rock or the phosphate fertilizer mixing and stirring that acidification is crossed banked up and reacted 5-6 days, is humic-acid kind phosphate fertilizer;
(2) organic matter material fertilizer (comprising municipal wastes or animal excrement or fur offal or agricultural byproducts offal and various biscuit):
1. the residue of municipal wastes under residue after the magnetic separation stage treatment through banking up more than spontaneous fermentation 8-15 days, passes through 10-30 mesh sieve again, and filtrate is planted matter for organic and corruption, is good organic fertilizer after drying or drying;
2. add part grass meal or saw foam or weathered coal soil in the animal excrement, through spontaneous fermentation in 5-10 days or added three grades of bacteria fermentation 3-5 of biological organophosphorus bacterial fertilizer days, be good organic fertilizer after drying or drying;
3. the animal skin offal was pulverized 10-30 mesh sieve, added bio-azotobacter fertilizer material microbial inoculum and three grades of bacteria fermentation 4-6 of organophosphorus bacterial fertilizer days, was good organic protein fertilizer after drying or drying.
5. biological compounded granular fertilizer of a kind of Nuisanceless high-efficient according to claim 1 and production method thereof is characterized in that the preparation of above-mentioned inorganic fertilizer and manufacture craft are:
(1) amount of allocating into of inorganic raw material is: 30-60
Nitrogen, phosphorus, potassium content proportioning are: 3-8: 2-6: 1-5
1. urea content is 46.6%, adds (mass parts) to be: 5-19 part;
2. phosphorus content is 14%, and add-on (mass parts) is 20-50;
3. KCE content is 58%, and add-on (mass parts) is: 6-14 part;
(2) the trace element amount of allocating into (mass parts) is: 2-8 part, and its cooperation ratio is:
1. sal epsom 0.5-2.5,2. lime carbonate 0.5-2.5,3. boric acid 0.05-0.15,4. zinc sulfate 0.15-0.25,5. manganous sulfate 0.25-0.35,6. ammonium molybdate 0.005-0.015,7. copper sulfate 0.5-0.15,8. Xanthohumate 0.15-0.25,9. rare earth 0.04-0.095;
Make mixing granulation:
Elder generation is with fertilizer and humic acid phosphate fertilizer adds in the mixing machine or adding flyash; adding bio-bacterial manure again stirs; thereafter adding inorganics mixes; the composite fertilizer's batch mixing that mixes is arrived the tablets press granulation by conveyor delivery; air stream drying or oven drying at low temperature promptly become the complete first compounded granular fertilizer of high-effect organic biological.
CN200910181133A 2009-10-12 2009-10-12 Green environment protective biological organic compound fertilizer and production method thereof Pending CN101671204A (en)

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