CN102898224B - Composite manure water preparation for culturing pearl mussel - Google Patents
Composite manure water preparation for culturing pearl mussel Download PDFInfo
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- CN102898224B CN102898224B CN201210341992.5A CN201210341992A CN102898224B CN 102898224 B CN102898224 B CN 102898224B CN 201210341992 A CN201210341992 A CN 201210341992A CN 102898224 B CN102898224 B CN 102898224B
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- water preparation
- rich water
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Abstract
The invention relates to a composite manure water preparation which comprises the following substances by weight percent: 80 to 92 % of decomposed chicken manure, 3 to 10 % of calcium superphosphate, 1 to 5 % of potassium dihydrogen phosphate, 1 to 2 % of dipotassium hydrogen phosphate, 0.5 to 5 % of calcium carbonate, 0.02 to 0. 5 % of zinc sulfate, 0.04 to 1 % of manganese sulfate, 0.1 to 0.6 % of copper sulphate, 0.5 to 1 % of magnesium sulfate, 0.1 to 0.7 % of ferrous sulfate heptahydrate, 0.02 to 0.2 % of ammonium molybdate, 0.02 to 0.35 % of boric acid, 0.01 to 0.3 % of citric acid and 0.3 to 0.5 % of melosira. In addition, the composite manure water preparation provided by the invention is added with live bacteria of Bacillus subtilis and bifidobacteria. According to the invention, by using the fermented duck manure as main raw materials, together with N, P, K, trace elements, etc. necessary for phytoplankton and zooplankton, the prepared organic manure water preparation is sufficient in organic nutrients and trace element nutrients, and reasonable in compatibility, and can quickly supplement small molecular organic debris needed by pearl mussel, and N, P, K and a variety of trace elements needed by the phytoplankton and the zooplankton.
Description
Technical field
The invention belongs to agriculture production product technique field, be specifically related to a kind of rich water compound formulation, the rich water compound formulation that a kind of applicable pearl culturing clam cultivation waters is used.
Background technology
At present, the rich water product of culture fishery is a lot, but mostly configures for fishery products such as fishes and shrimps.And the cultivation of pearl freshwater mussel requires with general breed variety difference, current rich water product can not meet the demand of pearl freshwater mussel to basic nutritive elements such as nitrogen, phosphorus, potassium, calcium.And, also have a lot of mineral elements and trace element also very important at the foster freshwater mussel fishing waters of growing cultured pearls, being formed with directly and indirectly of the growth on pearl culturing clam and pearl affects.For example, nitrogen, phosphorus, potassium are the necessary elements of phytoplankton growth and breeding, and plant plankton is the primary productivity of water body, and plankton and organic debris are the basic substances that pearl culturing clam is depended on for existence.When food organism in water are enriched, the nutritional sufficiency of pearl culturing clam, pearl also just looks soon, and quality is high.It is different that this and general aquaculture need to be thrown in bait.Consider that calcium (existing with calcium carbonate form) is the major ingredient of pearl culturing clam shell and pearl, therefore, the calcium contents that the aquaculture water of pearl culturing clam requires is higher than ortho-water product demand.Meanwhile, all require certain content for copper, zinc, manganese, iron etc.Therefore develop for the special rich water preparation of pearl culturing clam aquaculture water and there is very great meaning.
Summary of the invention
The object of this invention is to provide the rich water compound formulation that a kind of applicable pearl culturing clam cultivation waters is used, have that calcic is high, nutritious, fertilizer efficiency is both lasting rapidly, can go out plant plankton and zooplankton by high effect culture.
Rich water compound formulation of the present invention, is made up of the material of following weight percent: the duck excrement 80～92% that becomes thoroughly decomposed, calcium superphosphate 3～10%, potassium primary phosphate 1～5%, dipotassium hydrogen phosphate 1～2%, calcium carbonate 0.5～5%, zinc sulfate 0.02～0.5%, manganous sulfate 0.04～1%, copper sulfate 0.1～0.6%, magnesium sulfate 0.5～1%, iron vitriol 0.1～0.7%, ammonium molybdate 0.02～0.2%, boric acid 0.02～0.35%, citric acid 0.01～0.3%, melosira 0.3～0.5%.
Rich water compound formulation of the present invention, is also added with the viable bacteria of genus bacillus and bifidus bacillus, thereby can activates the duck excrement that becomes thoroughly decomposed before preparation uses, and makes the effect of preparation better.
Genus bacillus and the bifidus bacillus quantity in compound formulation is respectively 10
6～10
8cFU/kg.
The present invention utilizes duck excrement after fermentation as main raw material, coordinates plant plankton and the necessary N of zooplankton, P, K and trace element etc., the fertilizer water preparation of making, and organic nutrient and micronutrient abundance, coordinate rationally.Rich water compound formulation of the present invention can supplement the required small molecules organic debris of pearl culturing clam fast and plankton needs N, P, K and various trace elements.Its main raw material is taken from and is supported the waste material that duck industry produces, and turns waste into wealth, and avoided a large amount of wastings of resources, and preparation is simple and easy to use.Meanwhile, in preparation of the present invention, add magnesium sulfate, can better promote the absorption application of calcium ion.
Embodiment
The method that the preparation method of duck excrement of becoming thoroughly decomposed adopts nature to become thoroughly decomposed, becomes thoroughly decomposed after duck excrement is piled up, and stirs therebetween, and to duck excrement odorless, the brown that darkens to black has just completed the preparation of the duck excrement that becomes thoroughly decomposed.
For the specific content that increases melosira, in rich water agent of the present invention, be added with the live body of a small amount of melosira as algae kind, in the time using, can effectively improve the amount of melosira in aquaculture water like this, for the growth of pearl culturing clam provides sufficient bait.
In order to strengthen the effect of rich water preparation of the present invention, in the rich water preparation of preparation, be also optionally added into the viable bacteria of genus bacillus and bifidus bacillus.Wherein the substratum of genus bacillus is composed as follows: 1L distilled water+20g glucose+15g peptone+5g sodium-chlor+0.5g extractum carnis+20g agar;
Medium of Bifidobacterium is selected BS substratum, can buy from Qingdao Hai Bo Bioisystech Co., Ltd.
After the substratum of genus bacillus, bifidus bacillus is prepared, respectively bacterial classification is inoculated in substratum according to 2% inoculum size, after access, after 35 DEG C of incubated overnight 24～48h, or measuring OD600 value is within 0.5 o'clock, to stop cultivating.
Below in conjunction with embodiment, the present invention is described further and verification experimental verification.
Embodiment 1
The rich water preparation of the present embodiment, is made up of the raw material of following weight percent: fermentation duck excrement 89.7%, calcium superphosphate 3%, potassium primary phosphate 1%, dipotassium hydrogen phosphate 1%, calcium carbonate 1%, zinc sulfate 0.5%, manganous sulfate 1%, copper sulfate 0.5%, magnesium sulfate 1%, iron vitriol 0.5%, ammonium molybdate 0.2%, boric acid 0.2%, citric acid 0.1%.Above-mentioned component is added to stirrer stirring and evenly mixing, then add melosira algae liquid, air-dry, making the weight percent of melosira in rich water agent is 0.3%.
Melosira can be selected variation melosira, purchased from hydrobiont institute of Chinese Academy of Sciences algae kind storehouse, is numbered FACHB-1187, and substratum is selected D1 substratum.
Embodiment 2
The present embodiment and embodiment 1 difference are that the mass percent of raw material is different, and its preparation method is identical with embodiment 1.
Fermentation duck excrement 86.5%, calcium superphosphate 4%, potassium primary phosphate 1%, dipotassium hydrogen phosphate 2%, calcium carbonate 2.2%, zinc sulfate 0.5%, manganous sulfate 1.0%, copper sulfate 0.6%, magnesium sulfate 0.5%, iron vitriol 0.7%, ammonium molybdate 0.2%, boric acid 0.1%, citric acid 0.2%, melosira 0.5%.
Embodiment 3
The present embodiment and embodiment 1 difference are that the mass percent of raw material is different, and its preparation method is identical with embodiment 1.
Fermentation duck excrement 86.5%, calcium superphosphate 4%, potassium primary phosphate 1%, dipotassium hydrogen phosphate 2%, calcium carbonate 2.2%, zinc sulfate 0.5%, manganous sulfate 1.0%, copper sulfate 0.6%, magnesium sulfate 0.5%, iron vitriol 0.7%, ammonium molybdate 0.2%, boric acid 0.1%, citric acid 0.2%, melosira 0.5%.
Preparing after above-mentioned rich water preparation, the cultivation bacterium liquid of genus bacillus, bifidus bacillus is being added to stirring, making the quantity of two kinds of bacterium in compound formulation be respectively 10
6～10
8cFU/kg, makes the rich water agent of the present embodiment after being then dried.Wherein genus bacillus can be selected purchased from Chinese agriculture microbial strains preservation center, is numbered the bacterial strain of 1511C0001ACCC02728; Bifidus bacillus, purchased from Chinese agriculture microbial strains preservation center, is numbered 1511C0001ACCC11054.
Owing to having added the viable bacteria of probiotics in rich water agent, before rich water agent is used, bacterium also can carry out biological degradation processing again to fermentation duck excrement; Therefore, compare than the rich water agent of not adding bacterium, when use, have extra beneficial effect.
The using method of rich water compound formulation of the present invention is as follows:
By preparation water 1:10 dilution, the culture pond of splashing after being uniformly mixed, 1 meter of pond usage quantity of every mu of depth of water is 1-3Kg.Concrete usage quantity is depending on water colour, and the season of growth of the 5-10 month, every two weeks once.
The experimental study of rich water compound formulation:
Water test is trained in pearl freshwater mussel cultivation waters in Zhejiang Tiandirun Pearl Co., Ltd., 22 DEG C of water temperatures, 1 meter of the depth of water, the water surface is 5 mu, uses the rich water compound formulation 10Kg of embodiment 1, after 3 days or 10 days 1 part of each pond surrounding and central each water sampling, fully mix, get 1 L mixed sample and pour vial into, add 5% formaldehyde solution to fix, leave standstill 24-48h, concentrated after at plant plankton counting frame microscopy, counting.Test and in the sampling water quality of 3 days, detect altogether 6 23 of planktonic microalgae and belong to 30 kinds.Wherein, Chlorophyta 8 belongs to 10 kinds, and Bacillariophyta 5 belongs to 5 kinds, and Pyrrophyta 5 belongs to 7 kinds, and Euglenophyta 3 belongs to 4 kinds, and Cyanophyta 1 belongs to a kind, and Chrysophyta 1 belongs to a kind.Planktonic microalgae cell quantity is between 8.33 × 10
7ind/ L.In the water quality of sampling after 10 days, detect altogether 6 24 of planktonic microalgae and belong to 32 kinds.Wherein, Chlorophyta 8 belongs to 10 kinds, and Bacillariophyta 5 belongs to 6 kinds, and Pyrrophyta 5 belongs to 8 kinds, and Euglenophyta 3 belongs to 4 kinds, and Cyanophyta 1 belongs to 2 kinds, and Chrysophyta 2 belongs to 2 kinds.Planktonic microalgae cell quantity is between 4.51 × 10
8ind/ L.Use after compound formulation, the algae in pearl culturing clam cultivation waters is many 2 kinds, and planktonic microalgae cell quantity is 5 times before using, and has rolled up the bait of pearl culturing clam, lays a solid foundation for bringing out high-quality pearl.
Adopt the rich water agent of embodiment 2,3 to carry out identical test, also can reach rich water effect of the present invention, the object that provides pearl culturing clam to grow required plant plankton and zooplankton is provided.And owing to having added probiotics in embodiment 3, the nacreous sickness rate in cultivation is all lower than the preparation of embodiment 1 and 2.
Claims (2)
1. the rich water compound formulation that applicable pearl culturing clam cultivation waters is used, it is characterized in that, material by following weight percent forms: duck excrement 80～92% becomes thoroughly decomposed, calcium superphosphate 3～10%, potassium primary phosphate 1～5%, dipotassium hydrogen phosphate 1～2%, calcium carbonate 0.5～5%, zinc sulfate 0.02～0.5%, manganous sulfate 0.04～1%, copper sulfate 0.1～0.6%, magnesium sulfate 0.5～1%, iron vitriol 0.1～0.7%, ammonium molybdate 0.02～0.2%, boric acid 0.02～0.35%, citric acid 0.01～0.3%, melosira 0.3～0.5%; And be also added with the viable bacteria of genus bacillus and bifidus bacillus at rich water compound formulation.
2. rich water compound formulation as claimed in claim 1, is characterized in that described genus bacillus and the quantity of bifidus bacillus in compound formulation are respectively 10
6～10
8cFU/kg.
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| WO2016178644A1 (en) * | 2015-05-07 | 2016-11-10 | Олег Иванович МИНЕРАЛОВ | Method of producing an organic fertilizer |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101225007A (en) * | 2008-02-03 | 2008-07-23 | 武汉市科洋生物工程有限公司 | Special biological fish-fertilizer for aquaculture and preparation method thereof |
| CN101671204A (en) | 2009-10-12 | 2010-03-17 | 北京中国科学院老专家技术中心 | Green environment protective biological organic compound fertilizer and production method thereof |
| CN102304488A (en) * | 2011-09-19 | 2012-01-04 | 浙江天地润珍珠有限公司 | Microbial preparation for breeding hyriopsis schlegeli |
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2012
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101225007A (en) * | 2008-02-03 | 2008-07-23 | 武汉市科洋生物工程有限公司 | Special biological fish-fertilizer for aquaculture and preparation method thereof |
| CN101671204A (en) | 2009-10-12 | 2010-03-17 | 北京中国科学院老专家技术中心 | Green environment protective biological organic compound fertilizer and production method thereof |
| CN102304488A (en) * | 2011-09-19 | 2012-01-04 | 浙江天地润珍珠有限公司 | Microbial preparation for breeding hyriopsis schlegeli |
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