CN101663934B - Regulating and controlling method for improving germination rate of woody seeds - Google Patents
Regulating and controlling method for improving germination rate of woody seeds Download PDFInfo
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- CN101663934B CN101663934B CN2009101677117A CN200910167711A CN101663934B CN 101663934 B CN101663934 B CN 101663934B CN 2009101677117 A CN2009101677117 A CN 2009101677117A CN 200910167711 A CN200910167711 A CN 200910167711A CN 101663934 B CN101663934 B CN 101663934B
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Abstract
The invention provides a regulating and controlling method for improving germination rate of woody seeds, comprising the following steps: firstly, soaking the woody seeds for 10-30min by 75% of alcohol, and washing for 2-3 times by bacteria free water; secondly, placing the washed seeds into organic mixed solvent to soak for 8-24h, and air drying naturally, mixing seeds and seed dressing agent with blending agent, wherein, the mixture proportion of the seeds to the seed dressing agent is 1:10-20 in parts by weight, and packaging by seed bags; finally, placing the packaged seeds into an artificial intelligent climate box to culture for 48-72h at the temperature of 28+/-3 DEG C and relative humidity of 75%+/-5%. The method has low cost, simple operation, flexible implementation manner, can effectively shorten germination time, improve germination rate, and can be widely used in treatment before seeding of woody seeds of water conservation and gardens.
Description
Technical field:
The present invention relates to the woody method for treating seeds in a kind of improvement of the ecological environment field, particularly relate to a kind of regulate and control method that improves germination rate of woody seeds.
Background technology
All there is strong phenomenon in Woody plant seed mostly, the principal element that seed is strong to be the woody seed germination difficulty of influence, germination rate is low.Strong kind of the skin structure layer that mainly comes from of woody seed contains hydrophobic chemical substances such as cutin, colloid, wax, bolt matter and hemicellulose, and these material machineries fetter seeds, have a strong impact on permeable, the gas permeability of kind of skin.Therefore, abolishing seed strong is to shorten woody seed germinating time, improves the important technology direction of germination rate.It is more to remove the strong method of woody seed, mainly comprise physics, chemistry, biological 3 types, the most general method is mechanical friction, soda acid processing, emerge in worm water and HORMONE TREATMENT.License notification number CN 100435640C discloses a kind of Woody plant seed soaking agent, its prescription is made up of gibberellin, three decane alcohol, potassium nitrate, α-Nai Yisuan and paclobutrazol, thin up in use is mainly used in seed soaking, the vernalization of Woody plant seed.Its characteristics are mainly not take technical measures such as dissolving, decomposition or conversion at seed kind skin structure layer material with the hormone regulating and controlling germination rate of woody seeds.And the difficult control in aspects such as processing time, treatment temperature, concentration of treatment, effect is also undesirable.
Summary of the invention
The object of the invention is to overcome above-mentioned shortcoming, providing a kind of is background to analyze woody seed kind skin structure, from technical standpoints such as dissolving, decomposition and stimulations, can abolish the solid shell of woody seed, irritation cell division and growth, promote seed quick-speed germination, and the regulation and control processing method of the high raising germination rate of woody seeds of germination rate.
The step that the present invention improves the regulate and control method of germination rate of woody seeds is:
1) at first,, carries out sterilizing and handle, use sterile water wash 2-3 time again with mass percentage concentration 75% alcohol-pickled seed 10-30min;
2) the step 1) seeds treated is put into organic mixed solvent, natural air drying behind the seed soaking 8-24h;
3) with step 2) seeds treated and seed dressing mix, and encapsulate with seed packet.Wherein, mixed proportion is by being seed by weight: seed dressing=1: 10～20.
4) seed packet with the step 4) gained is put in the artificial intelligence climate box, cultivates 48～72h under 28 ℃ ± 3 ℃ of temperature, relative moisture 75% ± 5% condition.
Wherein, organic mixed solvent set of dispense than by weight is: absolute ethyl alcohol: n-hexane: vegetable oil APE: Wax removal water=40～70: 30～60: 2～8: 4～10.Vegetable oil APE is Fujian Province Quanzhou Desheng Pesticide Co., Ltd. commercially available prod.Wax removal water is the Shenzhen clean Science and Technology Ltd. of speed commercially available prod, and ProductName is the general Wax removal water of ST101; The seed dressing set of dispense is cellulase than by weight: gibberellin: nitric acid rare earth: turfy soil: pyrethrin: water=1～4: 0.005～0.02: 10～20: 50～70: 0.2～0.8: 10～20.Cellulase is a solid powder, is the grand mcroorganism Co., Ltd in Shandong commercially available prod, and shady and cool low temperature drying is deposited.Gibberellin is 40% gibberellin water-soluble granule, is Shanghai Tongrui Biotechnology Co., Ltd. commercially available prod, and shady and cool drying is deposited.Nitric acid rare earth is the sharp bio tech ltd of Linyi, Shandong Bert commercially available prod.The plant ash powder is Yuexi County, Sichuan Province humic processing factory commercially available prod, and pyrethrin is 1.5% pyrethrin wetting powder, is southern Red River Shenju Biology Co., Ltd commercially available prod.
The advantage that the present invention improves the regulate and control method of germination rate of woody seeds is 1) the present invention is technical background to analyze strong woody seed kind skin structure layer and main component, a plurality of technical measures such as sterilization, dissolving wax and high fat thing, cellulose and hemicellulose, irritation cell merisis, environmental simulation induction regulating controlling have been adopted, impel strong woody seed kind skin fast softening and dissolving, improved the vigor of seed germination greatly.2) the organic mixed solvent component absolute ethyl alcohol that relates to of the inventive method and n-hexane have certain dissolution to wax and oil substances simultaneously as the primary solvent carrier.Vegetable oil APE is as a kind of bleeding agent by force.Wax removal water has kind of skin wax and grease and dissolves function more by force.3) the plain enzyme of the seed dressing component fibre that relates to of the inventive method can catalyse cellulose, hemicellulose decomposes, and makes hardseed kind skin structure layer fast softening and hydrolysis, improves that kind of skin is ventilative, water penetration.Gibberellin is a kind of frequently seen plants growth regulator, and purpose is irritation cell division and tissue differentiation, promotes seed quick-speed germination.Nitric acid rare earth is to increase seed vitality, simultaneously to the auxiliary synergistic effect of gibberellin function performance.Pyrethrin and turfy soil powder can guarantee that seed does not have damage by disease and insect harm, provide seed germination the reasonable physical environment required with growth.4) seed packet is put into the artificial intelligence climate box and cultivated 48 ~ 72h (28 ℃ ± 3 ℃, RH75% ± 5%), purpose is that cellulose initiated enzyme produces higher vigor, helps cellulase that seed is played a role fast.In a word, the regulate and control method of raising germination rate of woody seeds provided by the invention, have that cost is reasonable, simple to operate, method of application is flexible, can effectively shorten sprout time, improve advantage such as germination rate, be widely used in the processing before water is protected the woody planting seed of class, gardens class.
Embodiment:
Embodiment 1:
Its operating procedure of regulate and control method that the present invention improves germination rate of woody seeds is as follows:
1) with 75% alcohol the China tree seed is soaked 30min, use sterile water wash 3 times behind the soaking disinfection;
2) the step 1) seeds treated is put into organic mixed solvent soak seed 24h, natural air drying again;
3) with step 2) seeds treated and seed dressing mix, and encapsulate with seed packet.Wherein, mixed proportion seed by weight: seed dressing=1: 15;
4) with step 3) gained seed packet in putting into the artificial intelligence climate box, in 31 ℃ of temperature, cultivate 72h under the condition of relative moisture 80%.
Wherein, organic mixed solvent set of dispense than by weight is:
Absolute ethyl alcohol: n-hexane: vegetable oil APE: Wax removal water=55: 60: 8: 10.
The seed dressing set of dispense than by weight is:
Cellulase: gibberellin: nitric acid rare earth: turfy soil: pyrethrin: water=4: 0.02: 15: 60: 0.2: 20.
Implementation result:
After the inventive method was handled the China tree seed, the direct labor broadcasted sowing on kind of row of seedlings, and the management of watering.The China tree sprout time is 12 days, and germination rate is 68%.Do not use the inventive method to handle seed, the China tree sprout time is 20 days, germination rate 35%.The China tree seed germination time after comparative result the present invention handles shortens 8 days, and germination rate improves 94% relatively.
Embodiment 2:
Its operating procedure of regulate and control method that the present invention improves germination rate of woody seeds is as follows:
1) with 75% alcohol the Chinese sumac seed is soaked 20min, use sterile water wash 2 times behind the soaking disinfection;
2) the step 1) seeds treated is put into organic mixed solvent soak seed 18h, natural air drying again;
3) with step 2) seeds treated and seed dressing mix, and mixed proportion is seed by weight: seed dressing=1: 20, and encapsulate with seed packet respectively;
4) seed packet with the step 3) gained is put in the artificial intelligence climate box, is 75% time cultivation 60h in 28 ℃ of temperature, relative moisture.
Wherein, organic mixed solvent set of dispense than by weight is:
Absolute ethyl alcohol: n-hexane: vegetable oil APE: Wax removal water=40: 45: 5: 7.
The seed dressing set of dispense than by weight is:
Cellulase: gibberellin: nitric acid rare earth: turfy soil: pyrethrin: water=2: 0.01: 20: 70: 0.8: 15.
Implementation result:
After the inventive method is handled the Chinese sumac seed, adopt the mode of sowing grass seeds by duster to construct, and the management of watering.The Chinese sumac sprout time is 7 days, and germination rate is 75%.The Chinese sumac seed germination time of not using the inventive method to handle is 15 days, germination rate 45%.Comparative result shows that the Chinese sumac seed germination time that the present invention handles shortens 8 days, and germination rate improves 67% relatively.
Embodiment 3:
Its operating procedure of regulate and control method that the present invention improves germination rate of woody seeds is as follows:
1) with 75% alcohol the white popinac seed is soaked 10min, use sterile water wash 2 times behind the soaking disinfection;
2) the step 1) seeds treated is put into organic mixed solvent soak seed 8h, natural air drying again;
3) with step 2) seeds treated and seed dressing mix, and mixed proportion is a seed: seed dressing=1: 10, and encapsulate with seed packet;
4) seed packet with the step 3) gained is put in the artificial intelligence climate box, and in 25 ℃ of temperature, relative moisture is to cultivate 48h under 70% condition.
Wherein, organic mixed solvent set of dispense than by weight is:
Absolute ethyl alcohol: n-hexane: vegetable oil APE: Wax removal water=70: 30: 2: 4.
The seed dressing set of dispense than by weight is:
Cellulase: gibberellin: nitric acid rare earth: turfy soil: pyrethrin: water=1: 0.005: 10: 50: 0.5: 10.
Implementation result
Behind the inventive method Processing Ag silk tree seed, adopt the sowing of sprayed construction technology, and the management of watering.The white popinac sprout time is 5 days, and germination rate is 87%.Not using the inventive method to handle seed germinating time is 8 days, germination rate 68%%.Comparative result shows that the white popinac seed germination time after the inventive method is handled shortens 3 days, and germination rate improves 28 relatively.
Claims (1)
1. regulate and control method that improves germination rate of woody seeds is characterized in that step is as follows:
1) at first,, uses sterile water wash 2-3 time again with mass percentage concentration 75% alcohol-pickled seed 10-30min;
2) the step 1) seeds treated is put into organic mixed solvent, natural air drying behind the seed soaking 8-24h, wherein, organic mixed solvent set of dispense than by weight is absolute ethyl alcohol: n-hexane: vegetable oil APE: Wax removal water=40～70: 30～60: 2～8: 4～10;
3) again with step 2) seeds treated and seed dressing mix, and encapsulate with seed packet, wherein, mixed proportion is seed by weight: seed dressing=1: 10～20, the seed dressing set of dispense than by weight is cellulase: gibberellin: nitric acid rare earth: turfy soil: pyrethrin: water=1～4: 0.005～0.02: 10～20: 50～70: 0.2～0.8: 10～20;
4) seed packet with the step 3) gained is put in the artificial intelligence climate box, cultivates 48～72h under 28 ℃ ± 3 ℃ of temperature, relative moisture 75% ± 5% condition.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009101677117A CN101663934B (en) | 2009-09-22 | 2009-09-22 | Regulating and controlling method for improving germination rate of woody seeds |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2009101677117A CN101663934B (en) | 2009-09-22 | 2009-09-22 | Regulating and controlling method for improving germination rate of woody seeds |
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| Publication Number | Publication Date |
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| CN101663934A CN101663934A (en) | 2010-03-10 |
| CN101663934B true CN101663934B (en) | 2011-05-11 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2009101677117A Expired - Fee Related CN101663934B (en) | 2009-09-22 | 2009-09-22 | Regulating and controlling method for improving germination rate of woody seeds |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103688624B (en) * | 2013-12-18 | 2015-02-25 | 天津泰达绿化集团有限公司 | Method for promoting quick sprouting of Chinese rose seeds |
| CN105027733B (en) * | 2015-07-28 | 2017-06-27 | 平顶山学院 | A kind of method for promoting Germination of Taxus mairei Seeds |
| CN105638371B (en) * | 2016-03-18 | 2019-01-18 | 信阳农林学院 | A kind of green propagation method of Gu tea tree |
| CN106718541B (en) * | 2016-12-01 | 2019-08-23 | 唐婧婵 | Method for direct seeding of Yinhua tree for forest culture and management in rocky desertification region |
| CN110679431A (en) * | 2019-11-05 | 2020-01-14 | 河北省农林科学院石家庄果树研究所 | Peach seed emergence substrate and seedling raising method |
| CN113951075B (en) * | 2021-12-02 | 2022-12-30 | 福州市农业科学研究所 | Method for breaking shells of sweet potato seeds and raising seedlings |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100435640C (en) * | 2006-08-03 | 2008-11-26 | 叶建军 | Woody plant seed soaking agent |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100435640C (en) * | 2006-08-03 | 2008-11-26 | 叶建军 | Woody plant seed soaking agent |
Non-Patent Citations (3)
| Title |
|---|
| 孟梁等.伞树种子催芽方法的研究.《林业科技开发》.2009,第23卷(第3期),第117-119. * |
| 许岳飞.银合欢硬实种子处理方法研究.《草业科学》.2006,第23卷(第8期),第58-62页. * |
| 许杜意等.蔷薇属植物种子休眠原因及催芽方法研究进展.《江西林业科技》.2008,(第1期),第44-45页. * |
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