CN101663405B - 对多核苷酸模板进行测序的方法 - Google Patents

对多核苷酸模板进行测序的方法 Download PDF

Info

Publication number
CN101663405B
CN101663405B CN2007800448514A CN200780044851A CN101663405B CN 101663405 B CN101663405 B CN 101663405B CN 2007800448514 A CN2007800448514 A CN 2007800448514A CN 200780044851 A CN200780044851 A CN 200780044851A CN 101663405 B CN101663405 B CN 101663405B
Authority
CN
China
Prior art keywords
template
primer
polynucleotide
sequence
nucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN2007800448514A
Other languages
English (en)
Chinese (zh)
Other versions
CN101663405A (zh
Inventor
罗伯托·里加蒂
托比亚斯·奥斯特
萨拉·法舍娜
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Solexa Ltd Great Britain
Illumina Cambridge Ltd
Original Assignee
Solexa Ltd Great Britain
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=38886966&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CN101663405(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Solexa Ltd Great Britain filed Critical Solexa Ltd Great Britain
Publication of CN101663405A publication Critical patent/CN101663405A/zh
Application granted granted Critical
Publication of CN101663405B publication Critical patent/CN101663405B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/119—Double strand sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/122—Massive parallel sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/513—Detection characterised by immobilisation to a surface characterised by the pattern of the arrayed oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/543—Detection characterised by immobilisation to a surface characterised by the use of two or more capture oligonucleotide primers in concert, e.g. bridge amplification
CN2007800448514A 2006-10-06 2007-10-08 对多核苷酸模板进行测序的方法 Active CN101663405B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US85021006P 2006-10-06 2006-10-06
US60/850,210 2006-10-06
US89891007P 2007-02-01 2007-02-01
US60/898,910 2007-02-01
PCT/GB2007/003798 WO2008041002A2 (en) 2006-10-06 2007-10-08 Method for sequencing a polynucleotide template

Publications (2)

Publication Number Publication Date
CN101663405A CN101663405A (zh) 2010-03-03
CN101663405B true CN101663405B (zh) 2013-04-24

Family

ID=38886966

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2007800448514A Active CN101663405B (zh) 2006-10-06 2007-10-08 对多核苷酸模板进行测序的方法

Country Status (6)

Country Link
US (8) US7754429B2 (US07754429-20100713-C00009.png)
EP (3) EP3670672A1 (US07754429-20100713-C00009.png)
CN (1) CN101663405B (US07754429-20100713-C00009.png)
DK (1) DK2084295T3 (US07754429-20100713-C00009.png)
ES (1) ES2424155T3 (US07754429-20100713-C00009.png)
WO (1) WO2008041002A2 (US07754429-20100713-C00009.png)

Families Citing this family (254)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2202322A1 (en) 2003-10-31 2010-06-30 AB Advanced Genetic Analysis Corporation Methods for producing a paired tag from a nucleic acid sequence and methods of use thereof
GB0514910D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Method for sequencing a polynucleotide template
US8192930B2 (en) * 2006-02-08 2012-06-05 Illumina Cambridge Limited Method for sequencing a polynucleotide template
US9540637B2 (en) 2008-01-09 2017-01-10 Life Technologies Corporation Nucleic acid adaptors and uses thereof
CN102016068A (zh) 2008-01-09 2011-04-13 生命科技公司 制备用于核酸测序的配对标签文库的方法
US8202691B2 (en) 2008-01-25 2012-06-19 Illumina, Inc. Uniform fragmentation of DNA using binding proteins
US20090226975A1 (en) * 2008-03-10 2009-09-10 Illumina, Inc. Constant cluster seeding
US8143030B2 (en) * 2008-09-24 2012-03-27 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
WO2009120374A2 (en) 2008-03-28 2009-10-01 Pacific Biosciences Of California, Inc. Methods and compositions for nucleic acid sample preparation
JP2011515102A (ja) 2008-03-28 2011-05-19 パシフィック バイオサイエンシーズ オブ カリフォルニア， インコーポレイテッド 核酸シーケンシング用組成物及び方法
US8628940B2 (en) 2008-09-24 2014-01-14 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
EP2291533B2 (en) 2008-07-02 2020-09-30 Illumina Cambridge Limited Using populations of beads for the fabrication of arrays on surfaces
US8383345B2 (en) 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
US8383369B2 (en) * 2008-09-24 2013-02-26 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
WO2010038042A1 (en) 2008-10-02 2010-04-08 Illumina Cambridge Ltd. Nucleic acid sample enrichment for sequencing applications
WO2010048337A2 (en) 2008-10-22 2010-04-29 Illumina, Inc. Preservation of information related to genomic dna methylation
US8993230B2 (en) * 2008-12-04 2015-03-31 Pacific Biosciences of Californ, Inc. Asynchronous sequencing of biological polymers
US8236532B2 (en) 2008-12-23 2012-08-07 Illumina, Inc. Multibase delivery for long reads in sequencing by synthesis protocols
US20100285970A1 (en) * 2009-03-31 2010-11-11 Rose Floyd D Methods of sequencing nucleic acids
EP2425240A4 (en) 2009-04-30 2012-12-12 Good Start Genetics Inc METHOD AND COMPOSITION FOR EVALUATING GENETIC MARKERS
US9085798B2 (en) 2009-04-30 2015-07-21 Prognosys Biosciences, Inc. Nucleic acid constructs and methods of use
US9309557B2 (en) 2010-12-17 2016-04-12 Life Technologies Corporation Nucleic acid amplification
US9309566B2 (en) 2010-12-17 2016-04-12 Life Technologies Corporation Methods, compositions, systems, apparatuses and kits for nucleic acid amplification
US9334531B2 (en) 2010-12-17 2016-05-10 Life Technologies Corporation Nucleic acid amplification
US10036063B2 (en) 2009-07-24 2018-07-31 Illumina, Inc. Method for sequencing a polynucleotide template
CN102597256B (zh) 2009-08-25 2014-12-03 伊鲁米那股份有限公司 选择和扩增多核苷酸的方法
US10174368B2 (en) 2009-09-10 2019-01-08 Centrillion Technology Holdings Corporation Methods and systems for sequencing long nucleic acids
WO2011032040A1 (en) 2009-09-10 2011-03-17 Centrillion Technology Holding Corporation Methods of targeted sequencing
US8182994B2 (en) * 2009-09-15 2012-05-22 Illumina Cambridge Limited Centroid markers for image analysis of high denisty clusters in complex polynucleotide sequencing
US20110195410A1 (en) * 2009-12-07 2011-08-11 Gene Check, Inc. Compositions and methods related to solid phase sequence detection and genotyping
WO2011071923A2 (en) 2009-12-07 2011-06-16 Illumina, Inc. Multi-sample indexing for multiplex genotyping
WO2011106368A2 (en) 2010-02-23 2011-09-01 Illumina, Inc. Amplification methods to minimise sequence specific bias
US8951940B2 (en) 2010-04-01 2015-02-10 Illumina, Inc. Solid-phase clonal amplification and related methods
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
PT2556171E (pt) 2010-04-05 2015-12-21 Prognosys Biosciences Inc Ensaios biológicos codificados espacialmente
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
EP2390350A1 (en) * 2010-05-27 2011-11-30 Centre National de la Recherche Scientifique (CNRS) Method of DNA sequencing by polymerisation
US9029103B2 (en) * 2010-08-27 2015-05-12 Illumina Cambridge Limited Methods for sequencing polynucleotides
CN103429754B (zh) * 2010-09-23 2016-08-10 桑特里莱恩科技控股公司 天然延伸平行测序
US8759038B2 (en) * 2010-09-29 2014-06-24 Illumina Cambridge Limited Compositions and methods for sequencing nucleic acids
EP2633069B1 (en) * 2010-10-26 2015-07-01 Illumina, Inc. Sequencing methods
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
WO2012061832A1 (en) 2010-11-05 2012-05-10 Illumina, Inc. Linking sequence reads using paired code tags
WO2012064975A1 (en) 2010-11-12 2012-05-18 Gen9, Inc. Protein arrays and methods of using and making the same
ES2548400T3 (es) * 2010-11-12 2015-10-16 Gen9, Inc. Métodos y dispositivos para la síntesis de ácidos nucleicos
JP5907990B2 (ja) 2010-12-17 2016-05-11 ライフ テクノロジーズ コーポレーション 核酸増幅のための方法、組成物、システム、装置およびキット
US9163281B2 (en) 2010-12-23 2015-10-20 Good Start Genetics, Inc. Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
EP2663655B1 (en) * 2011-01-14 2015-09-02 Keygene N.V. Paired end random sequence based genotyping
US9217132B2 (en) 2011-01-20 2015-12-22 Ibis Biosciences, Inc. Microfluidic transducer
DK3037536T3 (da) 2011-01-28 2020-01-13 Illumina Inc Oligonukleotiderstatning for di-taggede og retnings biblioteker
CA2825815C (en) 2011-01-31 2017-09-05 Illumina, Inc. Methods for reducing nucleic acid damage
WO2012106546A2 (en) 2011-02-02 2012-08-09 University Of Washington Through Its Center For Commercialization Massively parallel continguity mapping
WO2012106072A2 (en) * 2011-02-03 2012-08-09 Illumina, Inc. Methods for minimizing sequence specific bias
US20120252682A1 (en) 2011-04-01 2012-10-04 Maples Corporate Services Limited Methods and systems for sequencing nucleic acids
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
US9476095B2 (en) 2011-04-15 2016-10-25 The Johns Hopkins University Safe sequencing system
US9150916B2 (en) * 2011-06-24 2015-10-06 Beat Christen Compositions and methods for identifying the essential genome of an organism
WO2013019361A1 (en) 2011-07-07 2013-02-07 Life Technologies Corporation Sequencing methods
EP2944693B1 (en) 2011-08-26 2019-04-24 Gen9, Inc. Compositions and methods for high fidelity assembly of nucleic acids
US9228233B2 (en) 2011-10-17 2016-01-05 Good Start Genetics, Inc. Analysis methods
PL3363901T3 (pl) 2012-02-17 2021-07-05 Fred Hutchinson Cancer Research Center Kompozycje i sposoby dokładnej identyfikacji mutacji
CN102534040A (zh) * 2012-02-28 2012-07-04 盛司潼 一种增加读长的测序方法
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
US20130274129A1 (en) * 2012-04-04 2013-10-17 Geneart Ag Tal-effector assembly platform, customized services, kits and assays
US8209130B1 (en) 2012-04-04 2012-06-26 Good Start Genetics, Inc. Sequence assembly
US8812422B2 (en) 2012-04-09 2014-08-19 Good Start Genetics, Inc. Variant database
US10227635B2 (en) 2012-04-16 2019-03-12 Molecular Loop Biosolutions, Llc Capture reactions
EP4001427A1 (en) 2012-04-24 2022-05-25 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
JP6509727B2 (ja) 2012-06-25 2019-05-15 ギンゴー バイオワークス， インコーポレイテッド 核酸アセンブリおよび高処理シークエンシングのための方法
CN110777195A (zh) * 2012-07-13 2020-02-11 生命技术公司 采用一组snp的人身份识别
US9092401B2 (en) 2012-10-31 2015-07-28 Counsyl, Inc. System and methods for detecting genetic variation
CN104812947B (zh) 2012-07-17 2018-04-27 考希尔股份有限公司 检测遗传变异的系统和方法
CN102925471B (zh) * 2012-08-10 2014-09-03 内蒙古科技大学 非限制性构建无缝质粒表达载体的方法
EP2888371B1 (en) * 2012-08-24 2017-08-02 Life Technologies Corporation Methods, compositions, systems, apparatuses and kits for nucleic acid paired end sequencing
US9181583B2 (en) 2012-10-23 2015-11-10 Illumina, Inc. HLA typing using selective amplification and sequencing
CN104956225B (zh) 2012-10-29 2018-10-02 约翰·霍普金斯大学 卵巢和子宫内膜癌的帕帕尼科拉乌测试
EP2917368A1 (en) 2012-11-07 2015-09-16 Good Start Genetics, Inc. Methods and systems for identifying contamination in samples
CA2892646A1 (en) 2012-12-10 2014-06-19 Resolution Bioscience, Inc. Methods for targeted genomic analysis
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
CA3094792A1 (en) 2013-03-13 2014-09-18 Illumina, Inc. Methods and compositions for nucleic acid sequencing
WO2014143158A1 (en) 2013-03-13 2014-09-18 The Broad Institute, Inc. Compositions and methods for labeling of agents
EP2971159B1 (en) 2013-03-14 2019-05-08 Molecular Loop Biosolutions, LLC Methods for analyzing nucleic acids
US9255265B2 (en) 2013-03-15 2016-02-09 Illumina, Inc. Methods for producing stranded cDNA libraries
EP3005200A2 (en) 2013-06-03 2016-04-13 Good Start Genetics, Inc. Methods and systems for storing sequence read data
US9879313B2 (en) 2013-06-25 2018-01-30 Prognosys Biosciences, Inc. Methods and systems for determining spatial patterns of biological targets in a sample
KR102177225B1 (ko) 2013-07-01 2020-11-10 일루미나, 인코포레이티드 촉매-무함유 표면 작용화 및 중합체 그라프팅
ES2628485T3 (es) 2013-07-03 2017-08-03 Illumina, Inc. Secuenciación mediante síntesis ortogonal
US9116866B2 (en) 2013-08-21 2015-08-25 Seven Bridges Genomics Inc. Methods and systems for detecting sequence variants
US9898575B2 (en) 2013-08-21 2018-02-20 Seven Bridges Genomics Inc. Methods and systems for aligning sequences
JP2016533182A (ja) 2013-10-18 2016-10-27 セブン ブリッジズ ジェノミクス インコーポレイテッド 疾患に誘導された変異を同定するための方法およびシステム
WO2015058093A1 (en) 2013-10-18 2015-04-23 Seven Bridges Genomics Inc. Methods and systems for genotyping genetic samples
US10851414B2 (en) 2013-10-18 2020-12-01 Good Start Genetics, Inc. Methods for determining carrier status
US11041203B2 (en) 2013-10-18 2021-06-22 Molecular Loop Biosolutions, Inc. Methods for assessing a genomic region of a subject
US11049587B2 (en) 2013-10-18 2021-06-29 Seven Bridges Genomics Inc. Methods and systems for aligning sequences in the presence of repeating elements
WO2015058095A1 (en) 2013-10-18 2015-04-23 Seven Bridges Genomics Inc. Methods and systems for quantifying sequence alignment
US9092402B2 (en) 2013-10-21 2015-07-28 Seven Bridges Genomics Inc. Systems and methods for using paired-end data in directed acyclic structure
AU2014346399A1 (en) 2013-11-11 2016-06-02 Arizona Board Of Regents On Behalf Of Northern Arizona University Systems and methods for universal tail-based indexing strategies for amplicon sequencing
CA2931533C (en) * 2013-12-09 2023-08-08 Illumina, Inc. Methods and compositions for targeted nucleic acid sequencing
CN106414765A (zh) 2013-12-20 2017-02-15 Illumina公司 在片段化的基因组dna样品中保留基因组连接信息
WO2015105963A1 (en) 2014-01-10 2015-07-16 Seven Bridges Genomics Inc. Systems and methods for use of known alleles in read mapping
US9817944B2 (en) 2014-02-11 2017-11-14 Seven Bridges Genomics Inc. Systems and methods for analyzing sequence data
GB201403216D0 (en) * 2014-02-24 2014-04-09 Cambridge Epigenetix Ltd Nucleic acid sample preparation
WO2015175530A1 (en) 2014-05-12 2015-11-19 Gore Athurva Methods for detecting aneuploidy
GB201409777D0 (en) * 2014-06-02 2014-07-16 Illumina Cambridge Ltd Methods of reducing density-dependent GC bias in isothermal amplification
WO2015189636A1 (en) 2014-06-13 2015-12-17 Illumina Cambridge Limited Methods and compositions for preparing sequencing libraries
CN112430641A (zh) 2014-06-30 2021-03-02 亿明达股份有限公司 使用单侧转座的方法和组合物
WO2016040446A1 (en) 2014-09-10 2016-03-17 Good Start Genetics, Inc. Methods for selectively suppressing non-target sequences
GB201416106D0 (en) * 2014-09-11 2014-10-29 Illumina Cambridge Ltd Paired-end sequencing by attachment of the complementary strand
US20160085910A1 (en) 2014-09-18 2016-03-24 Illumina, Inc. Methods and systems for analyzing nucleic acid sequencing data
CA2999708A1 (en) 2014-09-24 2016-03-31 Good Start Genetics, Inc. Process control for increased robustness of genetic assays
US9558321B2 (en) 2014-10-14 2017-01-31 Seven Bridges Genomics Inc. Systems and methods for smart tools in sequence pipelines
CA3002133A1 (en) 2014-10-17 2016-04-21 Good Start Genetics, Inc. Pre-implantation genetic screening and aneuploidy detection
BR112017007912A2 (pt) 2014-10-17 2018-01-23 Illumina Cambridge Limited transposon de preservação de contiguidade
US10000799B2 (en) 2014-11-04 2018-06-19 Boreal Genomics, Inc. Methods of sequencing with linked fragments
GB201419731D0 (en) 2014-11-05 2014-12-17 Illumina Cambridge Ltd Sequencing from multiple primers to increase data rate and density
WO2016077350A1 (en) * 2014-11-11 2016-05-19 Illumina, Inc. Polynucleotide amplification using crispr-cas systems
EP3227461A1 (en) 2014-12-05 2017-10-11 Amyris, Inc. High-throughput sequencing of polynucleotides
US10066259B2 (en) 2015-01-06 2018-09-04 Good Start Genetics, Inc. Screening for structural variants
WO2016141294A1 (en) 2015-03-05 2016-09-09 Seven Bridges Genomics Inc. Systems and methods for genomic pattern analysis
CN113186256A (zh) 2015-04-10 2021-07-30 空间转录公司 生物样本的空间区别、多重核酸分析
DK3822365T3 (da) 2015-05-11 2023-02-06 Illumina Inc Platform til opdagelse og analyse af terapeutiske midler
US10275567B2 (en) 2015-05-22 2019-04-30 Seven Bridges Genomics Inc. Systems and methods for haplotyping
DK3303614T3 (da) 2015-05-29 2020-05-18 Illumina Cambridge Ltd Forbedret anvendelse af overfladeprimere i klynger
EP3323070B1 (en) 2015-07-14 2024-05-08 Personal Genome Diagnostics Inc. Neoantigen analysis
ES2745556T3 (es) 2015-07-29 2020-03-02 Progenity Inc Acidos nucleicos y métodos para detectar anomalías cromosómicas
AU2016298541B2 (en) 2015-07-30 2019-10-31 Illumina, Inc. Orthogonal deblocking of nucleotides
US11286531B2 (en) 2015-08-11 2022-03-29 The Johns Hopkins University Assaying ovarian cyst fluid
US10793895B2 (en) 2015-08-24 2020-10-06 Seven Bridges Genomics Inc. Systems and methods for epigenetic analysis
US10724110B2 (en) 2015-09-01 2020-07-28 Seven Bridges Genomics Inc. Systems and methods for analyzing viral nucleic acids
US10584380B2 (en) 2015-09-01 2020-03-10 Seven Bridges Genomics Inc. Systems and methods for mitochondrial analysis
ES2796501T3 (es) 2015-10-10 2020-11-27 Guardant Health Inc Métodos y aplicaciones de la detección de fusión de genes en el análisis de ADN sin células
US11347704B2 (en) 2015-10-16 2022-05-31 Seven Bridges Genomics Inc. Biological graph or sequence serialization
WO2017079461A2 (en) 2015-11-04 2017-05-11 The Translational Genomics Research Institute Systems and methods of diagnosing and characterizing infections
CN108472005B (zh) 2015-11-09 2021-09-14 瑞迪艾森有限公司 辐射屏蔽装置及其应用
AU2016353133B2 (en) 2015-11-11 2022-12-08 Resolution Bioscience, Inc. High efficiency construction of dna libraries
CN108779487A (zh) 2015-11-16 2018-11-09 普罗格尼迪公司 用于检测甲基化状态的核酸和方法
SG11201805119QA (en) 2015-12-17 2018-07-30 Guardant Health Inc Methods to determine tumor gene copy number by analysis of cell-free dna
US20170199960A1 (en) 2016-01-07 2017-07-13 Seven Bridges Genomics Inc. Systems and methods for adaptive local alignment for graph genomes
US10364468B2 (en) 2016-01-13 2019-07-30 Seven Bridges Genomics Inc. Systems and methods for analyzing circulating tumor DNA
US10460829B2 (en) 2016-01-26 2019-10-29 Seven Bridges Genomics Inc. Systems and methods for encoding genetic variation for a population
US10262102B2 (en) 2016-02-24 2019-04-16 Seven Bridges Genomics Inc. Systems and methods for genotyping with graph reference
WO2017160779A2 (en) 2016-03-14 2017-09-21 The Translational Genomics Research Institute Methods and kits to identify klebsiella strains
DK3436607T3 (da) 2016-03-28 2023-07-10 Ncan Genomics Inc Indfangning af koblede dupleksmål
US10961573B2 (en) 2016-03-28 2021-03-30 Boreal Genomics, Inc. Linked duplex target capture
US10160987B2 (en) 2016-04-07 2018-12-25 Rebecca F. McClure Composition and method for processing DNA
BR112019003704A2 (pt) 2016-08-25 2019-05-28 Resolution Bioscience Inc métodos para a detecção de alterações na cópia genômica em amostras de dna
US11250931B2 (en) 2016-09-01 2022-02-15 Seven Bridges Genomics Inc. Systems and methods for detecting recombination
EP3541951A4 (en) 2016-11-15 2020-06-03 Personal Genome Diagnostics Inc. NON-UNIQUE BARCODES IN A GENOTYPING TEST
US20190348149A1 (en) 2016-11-16 2019-11-14 Illumina, Inc. Validation methods and systems for sequence variant calls
US11268137B2 (en) 2016-12-09 2022-03-08 Boreal Genomics, Inc. Linked ligation
EP4253565A2 (en) 2017-01-24 2023-10-04 Vastogen, Inc. Methods for constructing copies of nucleic acid molecules
WO2018183942A1 (en) 2017-03-31 2018-10-04 Grail, Inc. Improved library preparation and use thereof for sequencing-based error correction and/or variant identification
AU2018254190A1 (en) * 2017-04-17 2019-10-31 Guoliang Fu Methods, compositions, and kits for preparing nucleic acid libraries
EP3615671B1 (en) 2017-04-23 2021-07-21 Illumina Cambridge Limited Compositions and methods for improving sample identification in indexed nucleic acid libraries
ES2929837T3 (es) 2017-04-23 2022-12-02 Illumina Cambridge Ltd Composiciones y métodos para mejorar la identificación de muestras en bibliotecas de ácidos nucleicos indexados
EP3615692A1 (en) 2017-04-23 2020-03-04 Illumina Cambridge Limited Compositions and methods for improving sample identification in indexed nucleic acid libraries
EP3615691B1 (en) 2017-04-23 2021-05-26 Illumina, Inc. Compositions and methods for improving sample identification in indexed nucleic acid libraries
CN108866173A (zh) * 2017-05-16 2018-11-23 深圳华大基因科技服务有限公司 一种标准序列的验证方法、装置及其应用
CA3075927A1 (en) 2017-09-20 2019-03-28 Regeneron Pharmaceuticals, Inc. Immunotherapy methods for patients whose tumors carry a high passenger gene mutation burden
WO2019060716A1 (en) 2017-09-25 2019-03-28 Freenome Holdings, Inc. SAMPLE EXTRACTION METHODS AND SYSTEMS
JP2020525887A (ja) 2017-10-16 2020-08-27 イルミナ インコーポレイテッド ディープラーニングベースのスプライス部位分類
NZ759818A (en) 2017-10-16 2022-04-29 Illumina Inc Semi-supervised learning for training an ensemble of deep convolutional neural networks
EP4289996A3 (en) * 2017-11-06 2024-01-17 Illumina Inc. Nucleic acid indexing techniques
AU2018375785A1 (en) 2017-11-30 2019-12-12 Illumina, Inc. Validation methods and systems for sequence variant calls
JP6862581B2 (ja) 2018-01-15 2021-04-21 イルミナ インコーポレイテッド 深層学習ベースのバリアント分類器
US11739373B2 (en) * 2018-02-05 2023-08-29 G1 Sciences, Llc Systems and methods for multiplexed analyte detection using antibody-oligonucleotide conjugates
US20190318806A1 (en) 2018-04-12 2019-10-17 Illumina, Inc. Variant Classifier Based on Deep Neural Networks
CN111065645A (zh) 2018-05-15 2020-04-24 伊鲁米纳公司 用于表面结合寡核苷酸的化学裂解和脱保护的组合物和方法
US20200251183A1 (en) 2018-07-11 2020-08-06 Illumina, Inc. Deep Learning-Based Framework for Identifying Sequence Patterns that Cause Sequence-Specific Errors (SSEs)
SG11202101934SA (en) 2018-07-30 2021-03-30 Readcoor Llc Methods and systems for sample processing or analysis
KR102165734B1 (ko) 2018-10-15 2020-10-14 일루미나, 인코포레이티드 심층 컨볼루션 신경망을 사전 훈련시키기 위한 심층 학습 기반 기술
NL2022043B1 (en) 2018-11-21 2020-06-03 Akershus Univ Hf Tagmentation-Associated Multiplex PCR Enrichment Sequencing
KR20210098842A (ko) 2018-12-05 2021-08-11 일루미나 케임브리지 리미티드 브릿지 증폭에 의한 클러스터 생성을 위한 방법 및 조성물
CN112639126A (zh) 2018-12-14 2021-04-09 伊卢米纳剑桥有限公司 降低测序期间用未标记的核苷酸的定相
CN112654714A (zh) * 2018-12-17 2021-04-13 伊卢米纳剑桥有限公司 用于测序的引物寡核苷酸
CN112639125A (zh) 2018-12-17 2021-04-09 伊卢米纳剑桥有限公司 用于多核苷酸测序的组合物
CA3103633A1 (en) 2018-12-18 2020-06-25 Illumina Cambridge Limited Methods and compositions for paired end sequencing using a single surface primer
TWI725686B (zh) 2018-12-26 2021-04-21 財團法人工業技術研究院 用於產生液珠的管狀結構及液珠產生方法
US11293061B2 (en) 2018-12-26 2022-04-05 Illumina Cambridge Limited Sequencing methods using nucleotides with 3′ AOM blocking group
EP3884071A4 (en) 2019-01-03 2022-04-06 Boreal Genomics, Inc. LINKED TARGET CAPTURE
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
WO2020146741A1 (en) 2019-01-10 2020-07-16 Selim Olcum Calibration of a functional biomarker instrument
US20200232011A1 (en) * 2019-01-22 2020-07-23 Mission Bio Methods of nucleic acid detection and primer design
NL2023316B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based sequencing
NL2023311B9 (en) 2019-03-21 2021-03-12 Illumina Inc Artificial intelligence-based generation of sequencing metadata
US11210554B2 (en) 2019-03-21 2021-12-28 Illumina, Inc. Artificial intelligence-based generation of sequencing metadata
NL2023314B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based quality scoring
WO2020191387A1 (en) 2019-03-21 2020-09-24 Illumina, Inc. Artificial intelligence-based base calling
NL2023310B1 (en) 2019-03-21 2020-09-28 Illumina Inc Training data generation for artificial intelligence-based sequencing
NL2023312B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based base calling
WO2020206170A1 (en) 2019-04-02 2020-10-08 Progenity, Inc. Methods, systems, and compositions for counting nucleic acid molecules
CA3138806A1 (en) 2019-05-22 2020-11-26 Dalia Dhingra Method and apparatus for simultaneous targeted sequencing of dna, rna and protein
US11667954B2 (en) 2019-07-01 2023-06-06 Mission Bio, Inc. Method and apparatus to normalize quantitative readouts in single-cell experiments
EP4010494A1 (en) 2019-08-08 2022-06-15 INSERM (Institut National de la Santé et de la Recherche Médicale) Rna sequencing method for the analysis of b and t cell transcriptome in phenotypically defined b and t cell subsets
WO2021041981A1 (en) 2019-08-28 2021-03-04 Assembly Biosciences, Inc. Compositions comprising bacterial species and methods related thereto
US20210189483A1 (en) * 2019-12-23 2021-06-24 Mgi Tech Co. Ltd. Controlled strand-displacement for paired end sequencing
AU2021208466A1 (en) 2020-01-13 2022-08-11 Fluent Biosciences Inc. Single cell sequencing
CN115362264A (zh) 2020-01-29 2022-11-18 10X基因组学有限公司 用于分析物检测的组合物和方法
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
WO2021155371A1 (en) * 2020-02-02 2021-08-05 Ultima Genomics, Inc. Nucleic acid molecules comprising cleavable or excisable moieties
US20210265018A1 (en) 2020-02-20 2021-08-26 Illumina, Inc. Knowledge Distillation and Gradient Pruning-Based Compression of Artificial Intelligence-Based Base Caller
WO2021168326A1 (en) 2020-02-21 2021-08-26 Readcoor, Llc Compositions, methods and systems for sample processing
EP4114966A1 (en) 2020-03-03 2023-01-11 Pacific Biosciences Of California, Inc. Methods and compositions for sequencing double stranded nucleic acids
US11768175B1 (en) 2020-03-04 2023-09-26 10X Genomics, Inc. Electrophoretic methods for spatial analysis
US20220316003A1 (en) * 2020-03-09 2022-10-06 Illumina, Inc. Methods for sequencing polynucleotides
US11866782B2 (en) 2020-03-16 2024-01-09 Fluent Biosciences Inc. Multi-omic analysis in monodisperse droplets
US11188778B1 (en) 2020-05-05 2021-11-30 Illumina, Inc. Equalization-based image processing and spatial crosstalk attenuator
US11787831B2 (en) 2020-06-22 2023-10-17 Illumina Cambridge Limited Nucleosides and nucleotides with 3′ acetal blocking group
WO2022029484A1 (en) 2020-08-06 2022-02-10 Agendia NV Methods of assessing breast cancer using circulating hormone receptor transcripts
WO2022029489A1 (en) 2020-08-06 2022-02-10 Agendia NV Systems and methods of using cell-free nucleic acids to tailor cancer treatment
US20220042108A1 (en) 2020-08-06 2022-02-10 Agendia NV Systems and methods of assessing breast cancer
US20220042107A1 (en) 2020-08-06 2022-02-10 Agendia NV Systems and methods of scoring risk and residual disease from passenger mutations
GB202012434D0 (en) 2020-08-10 2020-09-23 Univ Manchester Multiomic analysis of nanoparticle-coronas
GB202012433D0 (en) 2020-08-10 2020-09-23 Univ Manchester Nanoparticle-enabled analysis of cell-free nucleic acid in complex biological fluids
KR20230110606A (ko) 2020-11-26 2023-07-24 인쎄름 (엥스띠뛰 나씨오날 드 라 쌍떼 에 드 라 흐쉐르슈 메디깔) 감염원을 식별하는 방법
US11486001B2 (en) 2021-02-08 2022-11-01 Singular Genomics Systems, Inc. Methods and compositions for sequencing complementary polynucleotides
WO2022219128A1 (en) 2021-04-15 2022-10-20 INSERM (Institut National de la Santé et de la Recherche Médicale) In vitro tests allowing to identify the potential for mdm2 inhibitors to induce the selection of mutations in patients suffering from a myeloproliferative neoplasm
KR20240009435A (ko) 2021-05-20 2024-01-22 일루미나, 인코포레이티드 합성에 의한 시퀀싱을 위한 조성물 및 방법
US20230016633A1 (en) 2021-06-15 2023-01-19 Illumina, Inc. Hydrogel-free surface functionalization for sequencing
US11859241B2 (en) 2021-06-17 2024-01-02 Element Biosciences, Inc. Compositions and methods for pairwise sequencing
US11220707B1 (en) 2021-06-17 2022-01-11 Element Biosciences, Inc. Compositions and methods for pairwise sequencing
US11455487B1 (en) 2021-10-26 2022-09-27 Illumina Software, Inc. Intensity extraction and crosstalk attenuation using interpolation and adaptation for base calling
WO2023003757A1 (en) 2021-07-19 2023-01-26 Illumina Software, Inc. Intensity extraction with interpolation and adaptation for base calling
WO2023004357A1 (en) 2021-07-23 2023-01-26 Illumina, Inc. Methods for preparing substrate surface for dna sequencing
WO2023049215A1 (en) 2021-09-22 2023-03-30 Illumina, Inc. Compressed state-based base calling
US20230096386A1 (en) 2021-09-30 2023-03-30 Illumina Cambridge Limited Polynucleotide sequencing
AU2022371198A1 (en) 2021-10-20 2024-05-02 Illumina, Inc. Methods for capturing library dna for sequencing
CN117813390A (zh) 2021-12-16 2024-04-02 因美纳有限公司 用于表面结合的多核苷酸的金属定向裂解的方法
WO2023114397A1 (en) 2021-12-16 2023-06-22 Illumina, Inc. Hybrid clustering
WO2023114394A1 (en) 2021-12-17 2023-06-22 Illumina, Inc. Orthogonal hybridization
WO2023122491A1 (en) 2021-12-20 2023-06-29 Illumina Cambridge Limited Periodate compositions and methods for chemical cleavage of surface-bound polynucleotides
CA3222842A1 (en) 2021-12-20 2023-06-29 Oliver MILLER Periodate compositions and methods for chemical cleavage of surface-bound polynucleotides
US20230212667A1 (en) 2021-12-29 2023-07-06 Illumina Cambridge Limited Methods of nucleic acid sequencing using surface-bound primers
CN114457146A (zh) * 2022-01-28 2022-05-10 赛纳生物科技(北京)有限公司 一种固相介质表面双端扩增测序的方法
CN114438185A (zh) * 2022-01-28 2022-05-06 赛纳生物科技(北京)有限公司 一种芯片表面双端扩增测序的方法
US11639499B1 (en) 2022-03-08 2023-05-02 Watchmaker Genomics, Inc. Reverse transcriptase variants
WO2023175041A1 (en) 2022-03-15 2023-09-21 Illumina, Inc. Concurrent sequencing of forward and reverse complement strands on concatenated polynucleotides
WO2023175037A2 (en) 2022-03-15 2023-09-21 Illumina, Inc. Concurrent sequencing of forward and reverse complement strands on separate polynucleotides for methylation detection
WO2023183764A1 (en) 2022-03-22 2023-09-28 Illumina Cambridge Limited Substrate with orthogonally functional nanodomains
WO2023183937A1 (en) 2022-03-25 2023-09-28 Illumina, Inc. Sequence-to-sequence base calling
WO2023186872A1 (en) 2022-03-30 2023-10-05 Illumina Cambridge Limited Methods for chemical cleavage of surface-bound polynucleotides
WO2023187061A1 (en) 2022-03-31 2023-10-05 Illumina Cambridge Limited Paired-end re-synthesis using blocked p5 primers
WO2023239917A1 (en) 2022-06-09 2023-12-14 Illumina, Inc. Dependence of base calling on flow cell tilt
WO2024039516A1 (en) 2022-08-19 2024-02-22 Illumina, Inc. Third dna base pair site-specific dna detection
WO2024057280A1 (en) 2022-09-16 2024-03-21 Illumina Cambridge Limited Nanoparticle with polynucleotide binding site and method of making thereof
WO2024061799A1 (en) 2022-09-19 2024-03-28 Illumina, Inc. Deformable polymers comprising immobilised primers
US20240102067A1 (en) 2022-09-26 2024-03-28 Illumina, Inc. Resynthesis Kits and Methods
US20240124929A1 (en) 2022-09-30 2024-04-18 Illumina, Inc. Mesophilic compositions for nucleic acid amplification
US20240110234A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Amplification Compositions and Methods
US20240124914A1 (en) 2022-09-30 2024-04-18 Illumina, Inc. Thermophilic compositions for nucleic acid amplification
US20240110221A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Methods of modulating clustering kinetics
CN117004698A (zh) * 2023-07-17 2023-11-07 深圳赛陆医疗科技有限公司 双端测序方法和试剂盒

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10051564A1 (de) * 2000-10-18 2002-08-01 Axaron Bioscience Ag Neue Verfahren zur parallelen Sequenzierung eines Nukleinsäuregemisches an einer Oberfläche
EP1591541A2 (en) * 1997-04-01 2005-11-02 Solexa Ltd. Method of nucleic acid sequencing
WO2007010252A1 (en) * 2005-07-20 2007-01-25 Solexa Limited Method for sequencing a polynucleotide template
WO2007091077A1 (en) * 2006-02-08 2007-08-16 Solexa Limited Method for sequencing a polynucleotide template

Family Cites Families (79)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4292503A (en) 1979-05-14 1981-09-29 Emerson Electric Co. Split-band electric heater
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
EP0224126A3 (en) 1985-11-25 1989-02-01 The University of Calgary Covalently linked complementary oligodeoxynucleotides as universal nucleic acid sequencing primer linkers
CA1322442C (en) 1986-04-28 1993-09-28 Joginder N. Anand Tetrabromo bisphenol based polyestercarbonate membranes and method of using
WO1989001050A1 (en) 1987-07-31 1989-02-09 The Board Of Trustees Of The Leland Stanford Junior University Selective amplification of target polynucleotide sequences
SE8801070D0 (sv) * 1988-03-23 1988-03-23 Pharmacia Ab Method for immobilizing a dna sequence on a solid support
US6107023A (en) 1988-06-17 2000-08-22 Genelabs Technologies, Inc. DNA amplification and subtraction techniques
GB8818020D0 (en) 1988-07-28 1988-09-01 Ici Plc Method for amplification of nucleotide sequences
CA2004326A1 (en) 1988-12-23 1990-06-23 Nanibushan Dattagupta Assay of sequences using amplified genes
US5629158A (en) 1989-03-22 1997-05-13 Cemu Bitecknik Ab Solid phase diagnosis of medical conditions
US5800992A (en) 1989-06-07 1998-09-01 Fodor; Stephen P.A. Method of detecting nucleic acids
US5547839A (en) 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
US5302509A (en) 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
JP3096722B2 (ja) 1990-01-17 2000-10-10 日本ケミカルリサーチ株式会社 ポリメラーゼ・チェーン・リアクション用プライマーの作製法およびそれを用いるｄｎａの増幅法
US5118604A (en) 1990-06-15 1992-06-02 Yale University Restriction-fragment DNA probes and probe clusters
WO1993004199A2 (en) 1991-08-20 1993-03-04 Scientific Generics Limited Methods of detecting or quantitating nucleic acids and of producing labelled immobilised nucleic acids
DE69233719T2 (de) 1991-09-24 2009-01-02 Keygene N.V. Primer, Sätze und Restriktionsfragmente und deren Benutzung in selektiver Restriktionsfragmentenamplifikation
EP1382386A3 (en) 1992-02-19 2004-12-01 The Public Health Research Institute Of The City Of New York, Inc. Novel oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids
EP0656068B1 (en) 1992-07-24 1999-12-15 University Of South Australia Amplification and detection process
RU2048522C1 (ru) 1992-10-14 1995-11-20 Институт белка РАН Способ размножения нуклеиновых кислот, способ их экспрессии и среда для их осуществления
JPH07203998A (ja) 1994-01-26 1995-08-08 Hamamatsu Photonics Kk 核酸塩基配列決定方法
CN1152946A (zh) 1994-05-28 1997-06-25 特普尼尔医学有限公司 生产核酸的拷贝
US6090592A (en) 1994-08-03 2000-07-18 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid on supports
US6060288A (en) 1994-08-03 2000-05-09 Mosaic Technologies Method for performing amplification of nucleic acid on supports
US5641658A (en) 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
EP0830460A1 (en) 1995-04-11 1998-03-25 Trustees Of Boston University Solid phase sequencing of biopolymers
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
DE19515552A1 (de) 1995-04-27 1996-10-31 Europ Lab Molekularbiolog Simultane Sequenzierung von Nukleinsäuren
SE9504099D0 (sv) 1995-11-16 1995-11-16 Pharmacia Biotech Ab A method of sequencing
US5935788A (en) 1996-06-25 1999-08-10 Lifespan Biosciences, Inc. Subtractive hybridization techniques for identifying differentially expressed and commonly expressed nucleic acid
US5837466A (en) 1996-12-16 1998-11-17 Vysis, Inc. Devices and methods for detecting nucleic acid analytes in samples
US20020061532A1 (en) 1997-02-14 2002-05-23 Mosaic Technologies, Inc. Method and apparatus for performing amplification of nucleic acids on supports
ATE269908T1 (de) 1997-04-01 2004-07-15 Manteia S A Methode zur sequenzierung von nukleinsäuren
US6326489B1 (en) 1997-08-05 2001-12-04 Howard Hughes Medical Institute Surface-bound, bimolecular, double-stranded DNA arrays
US6087099A (en) 1997-09-08 2000-07-11 Myriad Genetics, Inc. Method for sequencing both strands of a double stranded DNA in a single sequencing reaction
US6511803B1 (en) 1997-10-10 2003-01-28 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
US20030022207A1 (en) 1998-10-16 2003-01-30 Solexa, Ltd. Arrayed polynucleotides and their use in genome analysis
AU6154299A (en) 1998-09-18 2000-04-10 Allied-Signal Inc. Method and system for removing carbon monoxide from a reformate gas stream for fuel cell application
AR021833A1 (es) * 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
EP1124990B1 (en) 1998-10-27 2006-01-18 Affymetrix, Inc. Complexity management and analysis of genomic dna
US20030108867A1 (en) 1999-04-20 2003-06-12 Chee Mark S Nucleic acid sequencing using microsphere arrays
US6300070B1 (en) 1999-06-04 2001-10-09 Mosaic Technologies, Inc. Solid phase methods for amplifying multiple nucleic acids
US6339147B1 (en) 1999-07-29 2002-01-15 Epoch Biosciences, Inc. Attachment of oligonucleotides to solid supports through Schiff base type linkages for capture and detection of nucleic acids
ATE317024T1 (de) 1999-12-29 2006-02-15 Keygene Nv Verfahren zur erzeugung von oligonukleotiden, im besonderen zur detektion amplifizierter restriktionsfragmente, die durch aflp erhalten wurden
AU2001251582A1 (en) 2000-04-14 2001-10-30 Lynx Therapeutics, Inc. Method and compositions for ordering restriction fragments
US20020012982A1 (en) 2000-07-13 2002-01-31 Invitrogen Corporation Methods and compositions for rapid protein and peptide extraction and isolation using a lysis matrix
AR031640A1 (es) 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
EP1256632A3 (en) 2001-05-07 2004-01-02 Smithkline Beecham Corporation High throughput polymorphism screening
US20030219770A1 (en) 2001-11-08 2003-11-27 Eshleman James R. Methods and systems of nucleic acid sequencing
JP2005518811A (ja) 2002-03-05 2005-06-30 ソレックサ リミテッド 表現型に関連するゲノム全域での配列変化を決定するための方法
GB0205153D0 (en) 2002-03-05 2002-04-17 Manteia S A Methods for detecting genome-wide sequence variations associated with a phenotype
US20040002090A1 (en) 2002-03-05 2004-01-01 Pascal Mayer Methods for detecting genome-wide sequence variations associated with a phenotype
EP3795577A1 (en) 2002-08-23 2021-03-24 Illumina Cambridge Limited Modified nucleotides
EP1560838B1 (en) 2002-08-23 2009-05-13 Illumina Cambridge Limited Labelled nucleotides
US20040126765A1 (en) 2002-12-27 2004-07-01 Adams Craig W. Method and compositions for sequencing nucleic acid molecules
DE602004024034D1 (de) 2003-01-29 2009-12-24 454 Corp Nukleinsäureamplifikation auf basis von kügelchenemulsion
US20060147935A1 (en) 2003-02-12 2006-07-06 Sten Linnarsson Methods and means for nucleic acid sequencing
GB0324456D0 (en) 2003-10-20 2003-11-19 Isis Innovation Parallel DNA sequencing methods
EP2202322A1 (en) * 2003-10-31 2010-06-30 AB Advanced Genetic Analysis Corporation Methods for producing a paired tag from a nucleic acid sequence and methods of use thereof
WO2005060345A2 (en) 2003-12-22 2005-07-07 Biotage Ab New method for sequencing-by-synthesis
EP2789383B1 (en) 2004-01-07 2023-05-03 Illumina Cambridge Limited Molecular arrays
GB0400584D0 (en) 2004-01-12 2004-02-11 Solexa Ltd Nucleic acid chacterisation
GB2412170A (en) 2004-03-16 2005-09-21 Plant Bioscience Ltd A method for enriching for target nucleic acids in a sample
GB2413796B (en) 2004-03-25 2006-03-29 Global Genomics Ab Methods and means for nucleic acid sequencing
DE102004049532A1 (de) * 2004-10-09 2006-04-13 Fischer, Achim, Dr. Sequenzierung von Nukleinsäuren über Strand Displacement
US20060078899A1 (en) 2004-10-12 2006-04-13 Scheffer Alicia F Methods and compositions for reducing label variation in array-based comparative genome hybridization assays
WO2006064199A1 (en) 2004-12-13 2006-06-22 Solexa Limited Improved method of nucleotide detection
EP2003214B1 (en) 2005-02-01 2013-04-10 AB Advanced Genetic Analysis Corporation Reagents, methods, and libraries for bead-based sequencing
ES2404311T3 (es) 2005-04-12 2013-05-27 454 Life Sciences Corporation Métodos para determinar variantes de secuencias usando secuenciación ultraprofunda
WO2007145612A1 (en) 2005-06-06 2007-12-21 454 Life Sciences Corporation Paired end sequencing
US8428882B2 (en) 2005-06-14 2013-04-23 Agency For Science, Technology And Research Method of processing and/or genome mapping of diTag sequences
GB0514935D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Methods for sequencing a polynucleotide template
GB0514936D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Preparation of templates for nucleic acid sequencing
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
WO2007076726A1 (en) 2006-01-04 2007-07-12 Si Lok Methods for nucleic acid mapping and identification of fine-structural-variations in nucleic acids and utilities
EP2021503A1 (en) 2006-03-17 2009-02-11 Solexa Ltd. Isothermal methods for creating clonal single molecule arrays
WO2007111937A1 (en) * 2006-03-23 2007-10-04 Applera Corporation Directed enrichment of genomic dna for high-throughput sequencing
US8921073B2 (en) 2006-06-23 2014-12-30 Illumina, Inc. Devices and systems for creation of DNA cluster arrays
WO2009032167A1 (en) * 2007-08-29 2009-03-12 Illumina Cambridge Method for sequencing a polynucleotide template

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1591541A2 (en) * 1997-04-01 2005-11-02 Solexa Ltd. Method of nucleic acid sequencing
DE10051564A1 (de) * 2000-10-18 2002-08-01 Axaron Bioscience Ag Neue Verfahren zur parallelen Sequenzierung eines Nukleinsäuregemisches an einer Oberfläche
WO2007010252A1 (en) * 2005-07-20 2007-01-25 Solexa Limited Method for sequencing a polynucleotide template
WO2007091077A1 (en) * 2006-02-08 2007-08-16 Solexa Limited Method for sequencing a polynucleotide template

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Jay Shendure.Accurate Multiplex Polony Sequencing of an Evolved Bacterial Genome.《Science》.2005,第309卷(第5741期),1728-1732. *
Patrick Ng et. al..Multiplex sequencing of paired-end ditags (MS-PET): a strategy for the ultra-high-throughput analysis of transcriptomes and genomes.《Nucl. Acids Res.》.2006,第34卷(第12期),e84.
Patrick Ng et. al..Multiplex sequencing of paired-end ditags (MS-PET): a strategy for the ultra-high-throughput analysis of transcriptomes and genomes.《Nucl. Acids Res.》.2006,第34卷(第12期),e84. *

Also Published As

Publication number Publication date
US9267173B2 (en) 2016-02-23
US7754429B2 (en) 2010-07-13
US10221452B2 (en) 2019-03-05
US8236505B2 (en) 2012-08-07
EP2084295B1 (en) 2013-05-22
EP2453023A3 (en) 2012-08-22
US20090088327A1 (en) 2009-04-02
US20120309634A1 (en) 2012-12-06
US8105784B2 (en) 2012-01-31
US20130274115A1 (en) 2013-10-17
WO2008041002A2 (en) 2008-04-10
CN101663405A (zh) 2010-03-03
DK2084295T3 (da) 2013-08-12
EP3670672A1 (en) 2020-06-24
US8431348B2 (en) 2013-04-30
EP2084295A2 (en) 2009-08-05
ES2424155T3 (es) 2013-09-27
US20120094848A1 (en) 2012-04-19
US20110223601A1 (en) 2011-09-15
EP2453023A2 (en) 2012-05-16
US20160090623A1 (en) 2016-03-31
WO2008041002A3 (en) 2008-06-19
US7960120B2 (en) 2011-06-14
US8765381B2 (en) 2014-07-01
US20110014657A1 (en) 2011-01-20
US20140336057A1 (en) 2014-11-13

Similar Documents

Publication Publication Date Title
CN101663405B (zh) 对多核苷酸模板进行测序的方法
US11634768B2 (en) Methods for indexing samples and sequencing multiple polynucleotide templates
CN101415839B (zh) 对多核苷酸模板进行测序的方法
US9999866B2 (en) Preparation of templates for nucleic acid sequencing
EP1910560B1 (en) Method for sequencing a polynucleotide template
CN111065645A (zh) 用于表面结合寡核苷酸的化学裂解和脱保护的组合物和方法

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant