CN101608241A - 用于检测人类K-ras基因突变的引物、探针及其使用方法 - Google Patents
用于检测人类K-ras基因突变的引物、探针及其使用方法 Download PDFInfo
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- CN101608241A CN101608241A CNA2009101115016A CN200910111501A CN101608241A CN 101608241 A CN101608241 A CN 101608241A CN A2009101115016 A CNA2009101115016 A CN A2009101115016A CN 200910111501 A CN200910111501 A CN 200910111501A CN 101608241 A CN101608241 A CN 101608241A
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突变名称 | 氨基酸变化 | 碱基变化 |
K-ras-M1 | Gly12Asp | GGT>GAT |
K-ras-M2 | Gly12Ala | GGT>GCT |
K-ras-M3 | Gly12Val | GGT>GTT |
K-ras-M4 | Gly12Ser | GGT>AGT |
K-ras-M5 | Gly12Arg | GGT>CGT |
K-ras-M6 | Gly12Cys | GGT>TGT |
K-ras-M7 | Gly13Asp | GGC>GAC |
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CN2009101115016A CN101608241B (zh) | 2009-04-13 | 2009-04-13 | 用于检测人类K-ras基因突变的引物、探针及其试剂盒 |
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CN101608241A true CN101608241A (zh) | 2009-12-23 |
CN101608241B CN101608241B (zh) | 2011-06-29 |
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Cited By (10)
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CN102094078A (zh) * | 2009-12-09 | 2011-06-15 | 上海宝藤生物医药科技有限公司 | 一种用于检测K-ras基因突变的引物及其应用 |
WO2011079524A1 (zh) * | 2009-12-30 | 2011-07-07 | 北京雅康博生物科技有限公司 | 用于定量检测k-ras突变的试剂盒 |
CN102367478A (zh) * | 2011-10-25 | 2012-03-07 | 浙江大学 | 用于KRAS基因突变分型的ARMS-qPCR检测试剂盒及检测方法 |
CN102453765A (zh) * | 2011-11-03 | 2012-05-16 | 厦门艾德生物医药科技有限公司 | Pik3ca基因驱动突变的检测探针、引物及试剂盒 |
CN102465180A (zh) * | 2010-11-19 | 2012-05-23 | 上海基康生物技术有限公司 | 大肠直肠癌相关基因K-ras基因突变检测方法和相关试剂盒 |
WO2012071985A1 (zh) * | 2010-12-02 | 2012-06-07 | 深圳华大基因科技有限公司 | 从ffpe样本中提取dna的方法及其用途 |
CN104862401A (zh) * | 2015-05-29 | 2015-08-26 | 沈阳优吉诺生物科技有限公司 | 检测kras基因热点突变位点的引物、试剂盒及其pcr方法 |
CN106755376A (zh) * | 2016-12-12 | 2017-05-31 | 厦门飞朔生物技术有限公司 | 一种用于检测b‑raf基因突变的多重荧光pcr检测试剂盒 |
CN106755375A (zh) * | 2016-12-12 | 2017-05-31 | 厦门飞朔生物技术有限公司 | 一种用于检测k‑ras基因突变的多重荧光pcr检测试剂盒 |
CN107287309A (zh) * | 2017-07-06 | 2017-10-24 | 罗保君 | 一种血液循环肿瘤dna点突变检测方法 |
Family Cites Families (5)
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KR100666099B1 (ko) * | 2004-04-27 | 2007-01-10 | 국립암센터 | K―ras 올리고뉴클레오티드 마이크로어레이 및 이를이용하여 K―ras 유전자의 돌연변이를 검사하는 방법 |
CN101423866A (zh) * | 2007-10-31 | 2009-05-06 | 上海长海医院 | 检测K-ras基因突变的PNA、探针、引物及方法 |
CN101434985B (zh) * | 2007-11-15 | 2012-12-19 | 上海长海医院 | K-ras基因突变的定量检测方法 |
CN101381779B (zh) * | 2008-10-21 | 2011-06-08 | 广州益善生物技术有限公司 | kRas基因突变的检测探针、液相芯片及其检测方法 |
CN101736080A (zh) * | 2008-11-24 | 2010-06-16 | 上海天昊生物科技有限公司 | 人类原癌基因kras的检测方法及试剂盒 |
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2009
- 2009-04-13 CN CN2009101115016A patent/CN101608241B/zh active Active
Cited By (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102094078A (zh) * | 2009-12-09 | 2011-06-15 | 上海宝藤生物医药科技有限公司 | 一种用于检测K-ras基因突变的引物及其应用 |
CN102094078B (zh) * | 2009-12-09 | 2013-03-20 | 上海宝藤生物医药科技有限公司 | 一种用于检测K-ras基因突变的引物及其应用 |
WO2011079524A1 (zh) * | 2009-12-30 | 2011-07-07 | 北京雅康博生物科技有限公司 | 用于定量检测k-ras突变的试剂盒 |
CN102465180A (zh) * | 2010-11-19 | 2012-05-23 | 上海基康生物技术有限公司 | 大肠直肠癌相关基因K-ras基因突变检测方法和相关试剂盒 |
WO2012071985A1 (zh) * | 2010-12-02 | 2012-06-07 | 深圳华大基因科技有限公司 | 从ffpe样本中提取dna的方法及其用途 |
CN102367478B (zh) * | 2011-10-25 | 2013-07-31 | 浙江大学 | 用于KRAS基因突变分型的ARMS-qPCR检测试剂盒及检测方法 |
CN102367478A (zh) * | 2011-10-25 | 2012-03-07 | 浙江大学 | 用于KRAS基因突变分型的ARMS-qPCR检测试剂盒及检测方法 |
CN102453765A (zh) * | 2011-11-03 | 2012-05-16 | 厦门艾德生物医药科技有限公司 | Pik3ca基因驱动突变的检测探针、引物及试剂盒 |
CN102453765B (zh) * | 2011-11-03 | 2013-07-24 | 厦门艾德生物医药科技有限公司 | Pik3ca基因驱动突变的检测探针、引物及试剂盒 |
CN104862401A (zh) * | 2015-05-29 | 2015-08-26 | 沈阳优吉诺生物科技有限公司 | 检测kras基因热点突变位点的引物、试剂盒及其pcr方法 |
CN106755376A (zh) * | 2016-12-12 | 2017-05-31 | 厦门飞朔生物技术有限公司 | 一种用于检测b‑raf基因突变的多重荧光pcr检测试剂盒 |
CN106755375A (zh) * | 2016-12-12 | 2017-05-31 | 厦门飞朔生物技术有限公司 | 一种用于检测k‑ras基因突变的多重荧光pcr检测试剂盒 |
CN107287309A (zh) * | 2017-07-06 | 2017-10-24 | 罗保君 | 一种血液循环肿瘤dna点突变检测方法 |
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