CN101568647B - 无需信号肽从细菌分泌抗体 - Google Patents

无需信号肽从细菌分泌抗体 Download PDF

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Publication number
CN101568647B
CN101568647B CN200680034693XA CN200680034693A CN101568647B CN 101568647 B CN101568647 B CN 101568647B CN 200680034693X A CN200680034693X A CN 200680034693XA CN 200680034693 A CN200680034693 A CN 200680034693A CN 101568647 B CN101568647 B CN 101568647B
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CN
China
Prior art keywords
antibody
gene
polypeptide
sequence
host cell
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Expired - Fee Related
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CN200680034693XA
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English (en)
Chinese (zh)
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CN101568647A (zh
Inventor
G·亚兰顿
C·R·贝宾顿
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Humanigen Inc
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Kalobios Pharmaceuticals Inc
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Publication of CN101568647A publication Critical patent/CN101568647A/zh
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C07K16/1203—Gram-negative bacteria
    • C07K16/1214—Pseudomonadaceae (F)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
CN200680034693XA 2005-07-22 2006-07-07 无需信号肽从细菌分泌抗体 Expired - Fee Related CN101568647B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US70190205P 2005-07-22 2005-07-22
US60/701,902 2005-07-22
US11/273,906 2005-11-14
US11/273,906 US8211648B2 (en) 2005-07-22 2005-11-14 Secretion of antibodies without signal peptides from bacteria
PCT/US2006/026334 WO2007018853A2 (en) 2005-07-22 2006-07-07 Secretion of antibodies without signal peptides from bacteria

Publications (2)

Publication Number Publication Date
CN101568647A CN101568647A (zh) 2009-10-28
CN101568647B true CN101568647B (zh) 2012-10-03

Family

ID=37679517

Family Applications (1)

Application Number Title Priority Date Filing Date
CN200680034693XA Expired - Fee Related CN101568647B (zh) 2005-07-22 2006-07-07 无需信号肽从细菌分泌抗体

Country Status (11)

Country Link
US (2) US8211648B2 (cg-RX-API-DMAC7.html)
EP (1) EP1907610B1 (cg-RX-API-DMAC7.html)
JP (1) JP5087541B2 (cg-RX-API-DMAC7.html)
KR (1) KR101014891B1 (cg-RX-API-DMAC7.html)
CN (1) CN101568647B (cg-RX-API-DMAC7.html)
AU (1) AU2006276823B2 (cg-RX-API-DMAC7.html)
CA (1) CA2615776C (cg-RX-API-DMAC7.html)
IL (1) IL188889A (cg-RX-API-DMAC7.html)
NZ (1) NZ565126A (cg-RX-API-DMAC7.html)
SG (1) SG177895A1 (cg-RX-API-DMAC7.html)
WO (1) WO2007018853A2 (cg-RX-API-DMAC7.html)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8211648B2 (en) * 2005-07-22 2012-07-03 Kalobios Pharmaceuticals, Inc. Secretion of antibodies without signal peptides from bacteria
JO3076B1 (ar) 2007-10-17 2017-03-15 Janssen Alzheimer Immunotherap نظم العلاج المناعي المعتمد على حالة apoe
KR101824512B1 (ko) 2008-04-28 2018-02-02 휴머니건, 아이엔씨. 과립구-대식세포 콜로니 자극 인자에 대한 항체
US20100272736A1 (en) 2009-02-04 2010-10-28 Kalobios Pharmaceuticals, Inc. Combination antibiotic and antibody therapy for the treatment of pseudomonas aeruginosa infection
WO2010093814A1 (en) 2009-02-11 2010-08-19 Kalobios Pharmaceuticals, Inc. Methods of treating dementia using a gm-csf antagonist
AU2010313621C1 (en) 2009-10-14 2015-11-26 Humanigen, Inc. Antibodies to EphA3
AR079336A1 (es) 2009-12-11 2012-01-18 Irm Llc Antagonistas de la pro-proteina convertasa-subtilisina/quexina tipo 9 (pcsk9)
MX2013009258A (es) 2011-02-11 2013-10-17 Novartis Ag Antagonistas de pcsk9.
JP5957443B2 (ja) * 2011-03-28 2016-07-27 長瀬産業株式会社 フェリチンの製造方法
WO2012170607A2 (en) 2011-06-10 2012-12-13 Novartis Ag Use of pcsk9 antagonists
WO2012168491A1 (en) 2011-06-10 2012-12-13 Novartis Ag Pharmaceutical formulations of pcsk9 antagonists
JP6415987B2 (ja) 2012-03-02 2018-10-31 アブリンクス エン．ヴェー． シュードモナス・エルギノーサのＰｃｒＶに結合する単一可変ドメイン抗体
WO2014084859A1 (en) 2012-11-30 2014-06-05 Novartis Ag Molecules and methods for modulating tmem16a activities
CN104995341B (zh) 2013-01-24 2018-09-07 埃布特拉斯有限公司 基于蛋白结合的fv文库及其制备方法
WO2015189477A1 (en) * 2014-06-13 2015-12-17 Tenboron Oy Conjugates comprising an anti-egfr1 antibody
RU2609627C2 (ru) * 2014-09-26 2017-02-02 Закрытое Акционерное Общество "Биокад" Высокоаффинные и агрегационно стабильные антитела на основе вариабельных доменов vl и производного vhh
MA41044A (fr) 2014-10-08 2017-08-15 Novartis Ag Compositions et procédés d'utilisation pour une réponse immunitaire accrue et traitement contre le cancer
US20190037816A1 (en) * 2016-01-13 2019-02-07 Regeneron Pharmaceuticals, Inc. Rodents having an engineered heavy chain diversity region

Citations (1)

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Publication number Priority date Publication date Assignee Title
US5843685A (en) * 1988-09-06 1998-12-01 Xoma Corporation Production of chimeric mouse-human antibodies with specificity to human tumor antigens

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US4816567A (en) * 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
US5618920A (en) * 1985-11-01 1997-04-08 Xoma Corporation Modular assembly of antibody genes, antibodies prepared thereby and use
US5223409A (en) * 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
ES2146572T3 (es) 1988-09-06 2000-08-16 Xoma Technology Ltd Elementos de expresion genica y la produccion de anticuerpos quimericos de raton-ser humano.
EP0404003A3 (en) 1989-06-19 1991-10-16 Xoma Corporation Chimeric mouse-human km10 antibody with specificity to a human tumor cell antigen
US5733743A (en) * 1992-03-24 1998-03-31 Cambridge Antibody Technology Limited Methods for producing members of specific binding pairs
US5914254A (en) * 1993-08-02 1999-06-22 Celtrix Pharmaceuticals, Inc. Expression of fusion polypeptides transported out of the cytoplasm without leader sequences
US5563046A (en) 1993-08-02 1996-10-08 Celtrix Pharmaceuticals, Inc. Fusion polypeptides and proteins
JPH07289267A (ja) * 1994-04-25 1995-11-07 Oriental Yeast Co Ltd 発現増強方法
US5824502A (en) * 1996-03-28 1998-10-20 Mitsui Chemicals, Inc. Method for secretory production of protein
JPH09313191A (ja) 1996-03-28 1997-12-09 Mitsui Petrochem Ind Ltd 蛋白質の分泌生産法
PT1157041E (pt) * 1999-03-01 2005-10-31 Genentech Inc Anticorpos para terapia e diagnostico de cancro
US7425328B2 (en) * 2003-04-22 2008-09-16 Purdue Pharma L.P. Tissue factor antibodies and uses thereof
DE10325026B4 (de) 2003-06-02 2013-04-18 Genencor International,Inc. Bakterien mit erhöhter Proteinsekretion, Nukleotidsequenzen codierend für ein SecA Protein mit erhöhter Proteinsekretion sowie Verfahren zur Produktion von Proteinen
US20040266993A1 (en) 2003-06-30 2004-12-30 Evans Glen A. Non-immunoglobulin binding polypeptides
CA2535971C (en) * 2003-08-20 2018-04-10 Biosite, Inc. Methods and compositions for measuring biologically active natriuretic peptides and for improving their therapeutic potential
US7981843B2 (en) * 2004-01-20 2011-07-19 Kalobios Pharmaceuticals, Inc. Antibody specificity transfer using minimal essential binding determinants
US8211648B2 (en) * 2005-07-22 2012-07-03 Kalobios Pharmaceuticals, Inc. Secretion of antibodies without signal peptides from bacteria

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5843685A (en) * 1988-09-06 1998-12-01 Xoma Corporation Production of chimeric mouse-human antibodies with specificity to human tumor antigens

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Alan I.Derman et al..A signal sequence is not required for protein export in prlA mutants of Escherichia coli.《The EMBO Journal》.1993,第12卷(第3期),参见第879页左列"摘要部分"，第880、881页左列倒数第一、二段
Alan I.Derman et al..A signal sequence is not required for protein export in prlA mutants of Escherichia coli.《The EMBO Journal》.1993,第12卷(第3期),参见第879页左列"摘要部分",第880、881页左列倒数第一、二段,第886页左列"Bacterial strains"部分,右列"Strain constructions"部分. *
ANTON V.ZAVIALOV et al..Secretion of Recombinant Proteins via the Chaperone/Usher pathway in Escherichia coli.《APPLIED AND ENVIRONMENTAL MICROBIOLOGY》.2001,第67卷(第4期),全文. *
第886页左列"Bacterial strains"部分，右列"Strain constructions"部分.

Also Published As

Publication number Publication date
AU2006276823A1 (en) 2007-02-15
US9127046B2 (en) 2015-09-08
US20070020685A1 (en) 2007-01-25
CN101568647A (zh) 2009-10-28
US20120309055A1 (en) 2012-12-06
CA2615776A1 (en) 2007-02-15
CA2615776C (en) 2014-05-13
NZ565126A (en) 2011-03-31
JP5087541B2 (ja) 2012-12-05
KR20080031452A (ko) 2008-04-08
EP1907610A4 (en) 2009-12-23
KR101014891B1 (ko) 2011-02-15
EP1907610B1 (en) 2013-09-11
IL188889A (en) 2014-09-30
IL188889A0 (en) 2008-04-13
AU2006276823B2 (en) 2010-04-22
EP1907610A2 (en) 2008-04-09
WO2007018853A3 (en) 2009-04-16
HK1113393A1 (en) 2008-10-03
US8211648B2 (en) 2012-07-03
WO2007018853A2 (en) 2007-02-15
JP2009510998A (ja) 2009-03-19
SG177895A1 (en) 2012-02-28

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Granted publication date: 20121003

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CF01 Termination of patent right due to non-payment of annual fee