CN101543265A - Artificial feed for larva of firma villosa - Google Patents
Artificial feed for larva of firma villosa Download PDFInfo
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- CN101543265A CN101543265A CN200810017833A CN200810017833A CN101543265A CN 101543265 A CN101543265 A CN 101543265A CN 200810017833 A CN200810017833 A CN 200810017833A CN 200810017833 A CN200810017833 A CN 200810017833A CN 101543265 A CN101543265 A CN 101543265A
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- acid
- solution
- chloride
- cobastab
- water
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- 241000510764 Villosa Species 0.000 title 1
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims abstract description 12
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- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 12
- GXCLVBGFBYZDAG-UHFFFAOYSA-N N-[2-(1H-indol-3-yl)ethyl]-N-methylprop-2-en-1-amine Chemical compound CN(CCC1=CNC2=C1C=CC=C2)CC=C GXCLVBGFBYZDAG-UHFFFAOYSA-N 0.000 claims abstract description 9
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- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 claims abstract description 3
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Landscapes
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
The invention relates to an artificial feed capable of meeting the growth and development requirements of 2-year-old and above larvae of anthurium andraeanum, which is an effective natural enemy of the insect pests of the longicorn, and is prepared from a plurality of amino acids such as alanine, arginine, aspartic acid and the like, and substances such as glucose, sucrose, biotin, choline chloride, vitamins, riboflavin, sodium chloride, malic acid, alpha-ketoglutaric acid, Yanhu carboxylic acid, a longicorn larva extracting solution, a yeast powder solution, a malt extracting solution, fresh milk, peptone, egg yolk, new bovine serum, chloramphenicol and the like, wherein indoor feeding experiments show that the anthurium andraeanum fed with the feed has an average growth period of 9.500 +/-0.548 d, an aged larva body length of 9.642 +/-0.732 mm, and can normally form cocoons and pupate, and the eclosion rate of 95-100%.
Description
Affiliated technical field
Content of the present invention belongs to the biological control technical field of forestry pest, relates to a kind ofly satisfying that the particularly natural enemy of anoplophora glabripennis and Monochamus alternatus of longicorn insect---hard 2 ages of first (dastarcus helophoroides) of flower suede and above larval growth are grown the man-made feeds of needs.
Background technology
The ancient title of longicorn Longhorn beetLe longicorn's larva, be commonly called as saw tree youth, it is a kind of crushing insect that endangers seeds such as China poplar, willow, elm, anoplophora glabripennis in the longicorn population is the disastrous trunk borer of the northern area of China forest, and Monochamus alternatus is that China south pine forest is propagated the trunk borer of pine wood nematode.Because longicorn ovum, larva, pupa in life all lived in the tree body, be difficult for being subjected to extraneous factors such as weather and human activity to influence, so difficulty of prevention and cure is very big.
The hard first Fairmaire of flower suede also claims that dastarcus helophoroides, the hard first of flower suede cave first, carpenter bee and the wing heavily fortified point of hanging are first-class, belong to coleoptera Colydiidae insect, be distributed in the scope of longitude 105～135, latitude 30～40, be China and Japanese peculiar, the hard first population of flower suede is spot distribution under the natural conditions, a little less than the diffusivity.The hard first of flower suede is to control the natural enemy insect of anoplophora glabripennis and Monochamus alternatus most worthy at present, by large scale artificial breeding and release, can control the harm of above-mentioned longicorn to forest.In the prior art, the breeding of the hard first of flower suede obtains and roundheaded borer is difficult mainly by means of roundheaded borer.In addition, in recent years, those skilled in the art also develop the man-made feeds of the hard first larva of some flower suedes in succession, as reports such as Wang Weidong be made into dastarcus longulus sharp larva artificial feed with dried silkworm chrysalis meal, dry yeast, benzoate, sorbic acid, propionic acid, inkfish oil (soybean oil) etc., its larva eclosion rate is 65.7%; The artificial larvae feeds that report such as Tabata.K is prepared with dried silkworm chrysalis meal, dry ferment, methyl-different-benzoic acid, peptone etc., its adult eclosion rate is 6.7%, and elder generation's Monochamus alternatus larva raising, the eclosion rate of raising with man-made feeds also only is 70% again, as seen utilize the rearing method success rate of having reported at present also lower, these have all limited the artificial propagation and the utilization of the hard first of colored suede to a great extent.
Summary of the invention
The objective of the invention is to the problem that prior art exists is solved, and then a kind of dastarcus longulus sharp larva artificial feed that significantly improves colored suede hard 2 ages of first and above larva artificial propagation and utilize effect is provided.
Another object of the present invention is to provide a kind of preparation and using method of above-mentioned dastarcus longulus sharp larva artificial feed.
In order to improve the eclosion rate of the hard first above larva in 2 age of colored suede, solve its a large amount of artificial feeding problems, the present invention is amino acid nutrient in the roundheaded borer body man-made feeds of the hard first larva of colored suede that become assignment system with reference to the hard first host of flower suede.It is to contain in every 100mL feed that the prescription of this feed is formed:
Basic culture solution 15～25mL, 50% roundheaded borer extract, 15～25mL,
10% dusty yeast solution, 1～10mL, 50% malt extracted solution, 1～10mL,
Fresh milk 10～20mL, 10% peptone solution, 1～10mL,
Egg yolk 5～15mL, new cow's serum 10～20mL,
Chloramphenicol 0.05～0.15g, vitamin E 1～10mg,
Water surplus,
Wherein, the basic culture solution of every 100mL is made of water and the preparation of following material: alanine 620～630mg, arginine 580～590mg, asparatate 1000～1010mg, glutamic acid 1610～1620mg, histidine 310～320mg, isoleucine 540～550mg, leucine 860～870mg, lysine 640～650mg, methionine 210～220mg, phenylalanine 530～540mg, proline 745～755mg, serine 550～560mg, glycine 520～530mg, cystine 90～100mg, threonine 510～520mg, tyrosine 910～920mg, valine 740～750mg, glucose 995～1005mg, sucrose 995～1005mg, fructose 195～205mg, biotin 0.01～0.10mg, Choline Chloride 195～205mg, Cobastab
120.01～0.05mg, folic acid 0.05～0.15mg, inositol 10～15mg, nicotinic acid 1～10mg, calcium pantothenate 1～5mg, Cobastab
60.10～0.50mg, riboflavin 0.50～1.50mg, thiamine 0.01～0.05mg, sodium chloride 745～755mg, potassium chloride 5～15mg, calcium chloride 15～25mg, sodium acid carbonate 15～25mg, malic acid 60～70mg, KG 30～40mg, butanedioic acid 1～10mg prolongs carboxylic acid 5～6mg recklessly.
Dastarcus longulus sharp larva artificial feed of the present invention can obtain by following preparation process:
(1). get alanine, arginine, asparatate, glutamic acid, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, glycine, cystine, threonine, tyrosine, valine, glucose, sucrose, fructose, biotin, Choline Chloride, Cobastab in the feed side by set of dispense than value
12, folic acid, inositol, nicotinic acid, calcium pantothenate, Cobastab
6, riboflavin, thiamine, sodium chloride, potassium chloride, calcium chloride, sodium acid carbonate, malic acid, KG, butanedioic acid, prolong recklessly that carboxylic acid and water are mixed with the capacity basic culture solution;
(2). roundheaded borer peeling back is dried in 60 ℃ of baking ovens, pulverize, adding distil water is mixed with 50% roundheaded borer extract;
(3). the bud that will sprout 3 days is about homogenate after the wheat seed weighing of 1cm, and the water of quality such as adding filters the back by qualitative filter paper and obtains 50% malt extracted solution;
(4). 50% roundheaded borer extract in basic culture solution and the prescription, 10% dusty yeast solution, 50% malt extracted solution, fresh milk, 10% peptone solution, egg yolk and new cow's serum are fully mixed, add chloramphenicol and vitamin E then, water is supplied surplus, is mixed with the hard first larvae feeds of colored suede of 100mL.
Method for breeding of the present invention is: make suitable artificial insect box with plastic foam, absorbent cotton is collected together into the bead of 0.5cm～0.8cm, absorbing above-mentioned man-made feeds reaches capacity, in the covering behind the feed coating, with insect needle the hard first larva of flower suede in 2 ages is chosen again and be put on the feed coating, thereby finish the inoculation of insect on this feed, in time add feed according to the food situation of getting of the hard first larva of flower suede then, cocoon, pupate until the hard first larva of flower suede; Collect resulting worm cocoon then, be cultured to emergence.
Compared with prior art, the present invention have prescription rationally, preparation and easy to operate, to advantages such as the hard first larva of flower suede propagating and breeding are effective.Observe through the raising experiment that the designer did, initial body length is 0.451 ± 0.051mm at the beginning of when raising flower suede heavily fortified point first 2 instar larvaes with man-made feeds of the present invention, it is 9.500 ± 0.548d that larva is on average got the food time, mature larva body length is 9.642 ± 0.732mm, the rate of cocooing of mature larva is 100%, flower suede hard cycle of sixty years is 95%～100% for adult eclosion rate, is much higher than the eclosion rate of existing like product; Through cocooing, pupate overall process, successfully turned out colored suede hard cycle of sixty years for adult indoor.
Description of drawings
Accompanying drawing is a dastarcus longulus sharp larva artificial feed preparation route map of the present invention.
The specific embodiment
Prescription embodiment: to the analysis result such as the table 1 of roundheaded borer polypide amino acid composition
Table 1 roundheaded borer polypide amino acid composition analysis result
According to above-mentioned analysis result, the material prescription such as the table 2 of basic culture solution in the specific embodiment of the present invention.
Table 2 basic culture solution prescription
Accurately take by weighing above-mentioned substance, mix the back and be mixed with basic culture solution 100mL with the distilled water constant volume; Get above-mentioned basic culture solution 20mL, add 50% roundheaded borer extract 20mL, 10% dusty yeast solution 6mL, malt extracted solution 6mL, fresh milk 16mL, 10% peptone solution 6mL, fresh egg yolk 10mL and the new abundant mixed dissolution of cow's serum 16mL again; Add 0.1g chloramphenicol and 5mg vitamin E then, finally be mixed with the hard first larvae feeds of colored suede of 100mL.
Plastic foam is made the cuboid of 3.5cm * 3.5cm * 2cm, and digging out a diameter thereon is the 1.5cm duck eye, makes artificial insect box.Absorbent cotton is collected together into the bead that diameter is about 0.5cm～0.8cm, with suction pipe feed is splashed in the cotton ball, reach capacity up to cotton ball, in the covering behind the feed coating, when the hard first newly hatched larvae of the colored suede on being inoculated into roundheaded borer is grown to 2 ages (body long average 0.451 ± 0.051), it is forwarded on the above-mentioned cotton balls that is filled feed raise, under 25 ℃ of conditions, cultivate and observe, the developmental state of the hard first of statistics flower suede every day, and in time change and inhale the cotton ball that feed is arranged, cocoon, pupate until the hard first larva of flower suede; Collect resulting worm cocoon then, be cultured to emergence.
The indoor feeding experiment shows that the first initial body length of raising the hard first second instar larvae of flower suede with these man-made feeds is 0.451 ± 0.051, and spending the hard first larva of suede on average to get the food time is 9.500 ± 0.548 days, and mature larva body length is 9.642 ± 0.732.Process through cocooing, pupating is successfully turned out colored suede hard cycle of sixty years for adult indoor, and the rate of cocooing of mature larva is 100%, and flower suede hard cycle of sixty years is 21.11% for adult eclosion rate.
Claims (3)
1, a kind of man-made feeds that can satisfy the hard first larval growth growth of colored suede needs is characterized in that containing in every 100mL feed:
Basic culture solution 15～25mL, 50% roundheaded borer extract, 15～25mL,
10% dusty yeast solution, 1～10mL, 50% malt extracted solution, 1～10mL,
Fresh milk 10～20mL, 10% peptone solution, 1～10mL,
Egg yolk 5～15mL, new cow's serum 10～20mL,
Chloramphenicol 0.05～0.15g, vitamin e1～10mg,
Water surplus,
Wherein, the basic culture solution of every 100mL is made of water and the preparation of following material: alanine 620～630mg, arginine 580～590mg, asparatate 1000～1010mg, glutamic acid 1610～1620mg, histidine 310～320mg, isoleucine 540～550mg, leucine 860～870mg, lysine 640～650mg, methionine 210～220mg, phenylalanine 530～540mg, proline 745～755mg, serine 550～560mg, glycine 520～530mg, cystine 90～100mg, threonine 510～520mg, tyrosine 910～920mg, valine 740～750mg, glucose 995～1005mg, sucrose 995～1005mg, fructose 195～205mg, biotin 0.01～0.10mg, Choline Chloride 195～205mg, Cobastab
120.01～0.05mg, folic acid 0.05～0.15mg, inositol 10～15mg, nicotinic acid 1～10mg, calcium pantothenate 1～5mg, Cobastab
60.10～0.50mg, riboflavin 0.50～1.50mg, thiamine 0.01～0.05mg, sodium chloride 745～755mg, potassium chloride 5～15mg, calcium chloride 15～25mg, sodium acid carbonate 15～25mg, malic acid 60～70mg, KG 30～40mg, butanedioic acid 1～10mg prolongs carboxylic acid 5～6mg recklessly.
2, a kind of method for preparing the described dastarcus longulus sharp larva artificial feed of claim 1 is characterized in that comprising following processing step:
2.1 get alanine, arginine, asparatate, glutamic acid, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, glycine, cystine, threonine, tyrosine, valine, glucose, sucrose, fructose, biotin, Choline Chloride, Cobastab in the feed side than value by set of dispense
12, folic acid, inositol, nicotinic acid, calcium pantothenate, Cobastab
6, riboflavin, thiamine, sodium chloride, potassium chloride, calcium chloride, sodium acid carbonate, malic acid, KG, butanedioic acid, prolong recklessly that carboxylic acid and water are mixed with the capacity basic culture solution;
2.2 roundheaded borer peeling back is dried in 60 ℃ of baking ovens, to pulverize, adding distil water is mixed with 50% roundheaded borer extract;
2.3 the bud that will sprout 3 days is about homogenate after the wheat seed weighing of 1cm, the water of quality such as adding filters the back by qualitative filter paper and obtains 50% malt extracted solution;
2.4 50% roundheaded borer extract in basic culture solution and the prescription, 10% dusty yeast solution, 50% malt extracted solution, fresh milk, 10% peptone solution, egg yolk and new cow's serum are fully mixed, add chloramphenicol and vitamin E then, water is supplied surplus, is mixed with the hard first larvae feeds of colored suede of 100mL.
3, preparation method according to claim 3 is characterized in that comprising following processing step: get alanine 624.20mg, arginine 585.20mg, asparatate 1002.40mg, glutamic acid 1614.40mg, histidine 318.00mg, isoleucine 542.80mg, leucine 866.80mg, lysine 646.80mg, methionine 214.00mg, phenylalanine 533.60mg, proline 750.00mg, serine 554.00mg, glycine 528.80mg, cystine 94.80mg, threonine 517.60mg, tyrosine 912.80mg, valine 744.40mg, glucose 1000.00mg, sucrose 1000.00mg, fructose 200.00mg, biotin 0.06mg, Choline Chloride 200.00mg, Cobastab
120.02mg, folic acid 0.10mg, inositol 15.00mg, nicotinic acid 5.0mg, calcium pantothenate 2.00mg, Cobastab
60.30mg, riboflavin 1.00mg, thiamine 0.02mg, sodium chloride 750.00mg, potassium chloride 10.00mg, calcium chloride 20.00mg, sodium acid carbonate 20.00mg, malic acid 67.00mg, KG 37.00mg, butanedioic acid 6.00mg, prolong recklessly that carboxylic acid 5.50mg and water are mixed with basic culture solution 100mL; Get above-mentioned basic culture solution 20mL, add 50% roundheaded borer extract 20mL, 10% dusty yeast solution 6mL, malt extracted solution 6mL, fresh milk 16mL, 10% peptone solution 6mL, fresh egg yolk 10mL and new cow's serum 16mL again and fully mix; Add 0.1g chloramphenicol and 5mg vitamin E then, be mixed with the hard first larvae feeds of colored suede of 100mL.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102613411A (en) * | 2012-04-09 | 2012-08-01 | 天津师范大学 | Artificial feed for chlorophorus diadema motschulsky and preparation method for artificial feed |
| CN102960559A (en) * | 2012-11-21 | 2013-03-13 | 南京林业大学 | Feed for artificially raising Dastarcus helophoroides in large scale and matched breeding technology thereof |
| CN106305631A (en) * | 2016-08-25 | 2017-01-11 | 仇颖超 | Breeding method of high-protein Hermetia illucens pupae |
| CN115176944A (en) * | 2022-07-29 | 2022-10-14 | 长江大学 | Spodoptera litura artificial feed added with calcium and magnesium elements and preparation method thereof |
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2008
- 2008-03-28 CN CN2008100178333A patent/CN101543265B/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102613411A (en) * | 2012-04-09 | 2012-08-01 | 天津师范大学 | Artificial feed for chlorophorus diadema motschulsky and preparation method for artificial feed |
| CN102613411B (en) * | 2012-04-09 | 2013-04-10 | 天津师范大学 | Artificial feed for chlorophorus diadema motschulsky and preparation method for artificial feed |
| CN102960559A (en) * | 2012-11-21 | 2013-03-13 | 南京林业大学 | Feed for artificially raising Dastarcus helophoroides in large scale and matched breeding technology thereof |
| CN106305631A (en) * | 2016-08-25 | 2017-01-11 | 仇颖超 | Breeding method of high-protein Hermetia illucens pupae |
| CN115176944A (en) * | 2022-07-29 | 2022-10-14 | 长江大学 | Spodoptera litura artificial feed added with calcium and magnesium elements and preparation method thereof |
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