CN101536641A - Guangxi zedoary florescence control method - Google Patents
Guangxi zedoary florescence control method Download PDFInfo
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- CN101536641A CN101536641A CN200910038659A CN200910038659A CN101536641A CN 101536641 A CN101536641 A CN 101536641A CN 200910038659 A CN200910038659 A CN 200910038659A CN 200910038659 A CN200910038659 A CN 200910038659A CN 101536641 A CN101536641 A CN 101536641A
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Abstract
The invention discloses a Guangxi zedoary florescence control method which comprises the following steps: (1), seedball treatment: collecting, screening, grading, cleaning, sterilizing and then airing Guangxi zedoary seedballs; (2), refrigeration: refrigerating the Guangxi zedoary seedballs in a refrigerator with the relative humidity of 70 percent to 95 percent at 13 DEG C to 20 DEG C for 30 days to 60 days; (3), germination: taking the seedballs out of the refrigerator and putting the seedballs into a greenhouse with the relative humidity larger than or equal to 90 percent at 25 DEG C to 30 DEG C for germination for 10 days to 30 days; and (4), flower culture: putting the germinated seedballs in a culture chamber for culture until bud bodies swell, and placing seedballs capable of forming blossom buds in natural conditions for culture when mauve inflorescences occur in green bracts but are not exposed. The method enables Guangxi zedoary which originally blooms between April and June and between July to September to bloom and fully bloom during the Spring Festival, thereby achieving the aim of controlling the florescence of the Guangxi zedoary and providing a high-grade new-year flower for the Spring Festival.
Description
Technical field
The present invention relates to a kind of plant florescence control method, especially relate to a kind of Guangxi zedoary florescence control method.
Background technology
Guangxi zedoary (Curcuma kwangsiensis) is Zingiber (Zingberaceae) turmeric (Curcuma) herbaceos perennial, has another name called a mao curcuma zedoary, osmanthus curcuma zedoary, belongs to a kind of subtropics flowering bulb, in South China's cultivation or wild.Guangxi zedoary plant height 0.5～1.1m, spike is extracted out or is extracted out in leaf sheath autumn from rhizome spring, inflorescence is peculiar gorgeous, shape such as pagoda, simple and elegant fragrance is arranged, having high ornamental value, is a kind of flowers resource of great exploitation potential for its, also meets the new flower plant of International Society for Horticultural Science's definition.Yet, at present to the research of Guangxi zedoary more be its medical value, less as the research of ornamental flower, rarely seen report that has the South China Botanical Garden from Guangxi zedoary, to filter out palace powder root tuber of aromatic turmeric.
Because Guangxi zedoary mainly breeds with rhizome, the florescence is 4～June and 7～September under field conditions (factors), and October, blade began yellow, and February in November to next year, the ground ball of sowing was in hibernation, and bud began to break up when 3～April of spring, temperature reached 25 ℃.When plant is withered when entering dormancy, rhizome has been dug, remove earth, cut off fibrous root, be placed on the storage of shady and cool ventilation place after drying, plant the beginning of spring in next year again, so can't satisfy the demand of blooming the Spring Festival.
Be maximum season of middle national expenditures flower amount the Spring Festival, if can be with Guangxi zedoary florescence control to the Spring Festival, then be a kind of high-grade annual flower grass that should save very much, do something for the occasion, be the highest embodiment of its ornamental value and economic benefit, this is to China's Resources of Wild development and use and advance the flower variety production domesticization significant.But do not see the research report of relevant Guangxi zedoary cultivation and florescence control aspect so far.
Summary of the invention
The purpose of this invention is to provide a kind of Guangxi zedoary florescence control method, promptly Guangxi zedoary kind ball refrigerated and vernalization is handled, with the florescence control of Guangxi zedoary to the Spring Festival, to realize the winter jasmine cultivation.
The objective of the invention is to realize by following technical measures: a kind of Guangxi zedoary florescence control method, it may further comprise the steps:
(1) processing of kind ball: collection, screening, classification, cleaning and sterilizing Guangxi zedoary seedballs, dry then;
(2) refrigeration: Guangxi zedoary kind ball is placed on 13～20 ℃, and refrigeration is 30～60 days in the refrigerating chamber of relative moisture (RH) 70～95%;
(3) vernalization: will plant ball and take out from refrigerating chamber, and put 25～30 ℃ into, vernalization is 10～30 days in the greenhouse of relative moisture (RH) 〉=90%;
(4) Hua cultivation: the kind ball after the vernalization is positioned over is cultured to sprout in the culturing room and expands, in the phyllary of green, reveal the aubergine inflorescence, but inflorescence can place the kind ball that forms bud under the natural conditions and cultivate when not exposing.
Guangxi zedoary kind ball November or begin to enter resting stage, at this moment, kind of ball is placed under 13～20 ℃ low temperature preserves, the respiration of kind of ball is died down, this moment, starch was hydrolyzed into carbohydrate, wherein only have the small part carbohydrate to be used to keep the metabolism of kind of ball, and major part was kept in kind of the ball, for the sprouting of kind of ball provides a large amount of energy substances, the therefore kind ball of handling through the refrigeration dormancy that can break kind of ball in a short time; Kind ball after will refrigerating afterwards is placed under 25～30 ℃ of temperature, vernalization can impel kind of ball to germinate and induce flower bud differentiation; Be cultured to sprout at last and expand in culturing room, reveal the aubergine spike in the green phyllary, can form irreversible bud this moment.
Kind ball in the step of the present invention (4) after the vernalization is positioned over the training method of cultivating in the culturing room and adopts water planting to cultivate; At this moment, the condition of described culturing room is: 25～32 ℃, and relative moisture (RH) 60～90%, intensity of illumination 45 μ mol m
-2s
-1, periodicity of illumination is 12 hours/day～24 hours/day.
The kind ball that will form bud in the step of the present invention (4) places best cultivation foster or water planting cultivation for the soil cultivation under the natural conditions.
When the training method of cultivating under cultivation of the culturing room in the step (4) and the natural conditions adopted water planting to cultivate, the water planting level was about 1/3～1/2 of kind of ball height.
Preferred 13～17 ℃ of the temperature of refrigeration in the step of the present invention (2), the best is 14～17 ℃.Preferred 40～60 days of described cold preservation time, the best is 50～60 days.
The described natural conditions of step of the present invention (4) are meant the natural conditions in subtropical zone winter or spring, also can be the artificial conditions of similarities of copying, and promptly temperature is more than 10 ℃, and humidity is more than 40%, light intensity 15 μ mol m
-2s
-1More than, periodicity of illumination is more than 9 hours/day.
The present invention can do following improvement: can survive for a long time at low temperatures in order to protect kind of ball, and the moisture that abundance is provided for kind of ball when vernalization, in step (2) refrigeration and step (3) vernalization, all on kind of ball, cover clean and loose culture medium; Spray water twice every day during refrigeration or the vernalization.
Described culture medium can be a kind of through in sand, peat soil and the perlite of sterilization, also can be any two or three mixing in them; Moistening loose culture medium can be kind of ball enough oxygen and moisture is provided, and helps the breathing of kind of ball and the bud demand to moisture in process of growth.And moisture can make kind of soft and soggyization of ball kind, increases water penetration and gas permeability, makes embryo be easy to break through, and helps the reinforcement of blastocyte metabolic activity, strengthens the active and various biochemical reactions of respiration, enzyme; Transportation and utilization that the organic matter that moisture helps preserving in kind of the ball is decomposed into simple soluble compound and helps these materials.When kind of ball was sprouted, respiration strengthened, and needed to suck a large amount of oxygen, the nutriment of preserving in the cell such as glucose gradually oxidation Decomposition be carbonic acid gas and water, and give off energy for various physiological activity utilizations.
The present invention also can do following improvement: in step (4), when the kind ball is successively cultivated under culturing room's cultivation and natural conditions, added nutrient solution every 8～12 days, guarantee that Guangxi zedoary obtains the nutrition of comprehensive and reasonable, also can improve dissolved oxygen to greatest extent, promote its growth for Guangxi zedoary provides sufficient oxygen, and help blooming.
Described nutrient solution is selected the MS nutrient solution for use, and it is a kind of low conductivity nutrient solution, contains macroelement and trace elements such as the required nitrogen of plant, phosphorus, potassium, zinc, copper, molybdenum, iron.Because the nutrient solution of variable concentrations is bloomed to the growth of Guangxi zedoary and had a significant impact, concentration is low excessively, influences the normal absorption of Guangxi zedoary, even may produce concentration difference and cause the Guangxi zedoary dehydration; Then can suppress growth during high concentration, even make plant death.Therefore, the concentration of described MS nutrient solution is 1/4～1/32 times of MS nutrient solution mother liquid concentration, and preferred concentration is 1/4～1/16 times of MS nutrient solution mother liquid concentration.
Described kind of ball of step of the present invention (1) selects new then ball to be advisable, and is chosen in the kind ball of the Guangxi zedoary acrial part of gathering in October and November then after withered.
Because the germination rate and the Protein content of Guangxi zedoary just have tangible correlation, and the kind ball is big more, protein content in its body is high more, therefore, it is 2～6cm kind ball that of the present invention kind of ball selected diameter for use, wherein preferred diameter is 3～6cm kind ball, and the best is selected the kind ball of 4～6cm for use, also is fine above 6cm if plant bulb diameter.
Guangxi zedoary florescence control method of the present invention, beneficial effect is as follows:
The experiment of following mask body and experimental data can make those skilled in the art of the present technique more fully understand the present invention, but do not limit the present invention in any way:
One, experiment content:
To plant ball and be divided into 2 classes:
Planted ball last year always; Novel species ball then.The novel species ball is divided into 4 grades by diameter then: 1 grade (planting 4～6cm), 2 grades of bulb diameters (plants 3～4cm), 3 grades of bulb diameters and (plants 2～3cm), 4 grades of bulb diameters (it is following to plant bulb diameter 2cm), kind ball after selecting all was soaked in 800 times the tpn solution 10 minutes, take out dry standby.
Testing index:
Plant ball storage and vernalization and use SANYO Incubator MIR-553 constant incubator, culturing room's condition of water planting is: 30 ± 2 ℃, and 70 ± 10%RH, intensity of illumination 45 μ mol m
-2s
-1, periodicity of illumination 24 hours/day.Handle 50 kind balls.Record is designated as DTB (Days to flowering), DTL (Daysto leaf opening) and DTS (Days to flowering senescence) respectively from being cultured to the fate of beginning flower (inflorescence base portion first little the flowers are in blossom put), Zhan Ye (the 1st blade opens) and wilting (on the inflorescence 50% bract wilting be end-of-life); Measure the spray height; The spray rugosity.Statistics flowering rate (the kind nodule number of the kind nodule number/processing of blooming); Rotting rate (the kind nodule number of rotten kind ball number/processing); The spray life-span (beginning, flower was to wilting fate).
The bract color and luster is divided into 3 grades:
0: only inflorescence top bract is an aubergine, and other parts are lavender or are bordering on white;
1: 1/3～2/3 bract is an aubergine on the inflorescence, and color and luster is bright-coloured inadequately bright;
2: to be bright-coloured aubergine and color and luster bright for 2/3 above bract on the inflorescence.
Statistical analysis
Adopt SPSS 11.0 softwares to carry out LSD and analyze, the equal difference of gained result is (P＜0.05) significantly.
1. plant the influence that the ball rank is bloomed to Guangxi zedoary
With other novel species ball of 4 levels and last year the old ball of planting all in 15 ℃ of refrigerations 40 days down, place 30 ℃ of vernalization after 30 days again, water planting in culturing room is observed kind of the influence (referring to table 1) that the ball rank is bloomed to Guangxi zedoary.
Table a kind of the influence that the ball rank is bloomed to Guangxi zedoary
| Plant the ball rank | Flowering rate (%) | Water planting is to beginning flower fate DTB | The spray life-span (my god) | Spray height (cm) | Spray rugosity (cm) |
| Old ball | 0 | - | - | - | - |
| 1 grade | 52.0 | 18 | 50 | 26.52 | 1.46 |
| 2 grades | 56.0 | 25 | 35 | 22.82 | 1.29 |
| 3 grades | 15 | 31 | 27 | 19.13 | 1.11 |
| 4 grades | 0 | - | - | - | - |
As can be seen from Table 1, planting ball and 4 grades of kind ball flowering rate always is zero, all fails to bloom.1,2 grades of kind ball flowering rate are the highest, and the utmost point is significantly higher than 3 grades of kind balls.1 grade of kind ball is than 7 days, 13 days in advance respectively 2 grades, 3 grades kind ball early flowering seasons, long 15 days, 23 days respectively spray life-span, 3 other kind of level ball early flowering seasons and spray life-span significant difference.Spray height and spray rugosity are successively decreased successively from 1 grade of kind ball to 3 grade kind ball, and significant difference.
2. storage and vernalization are to the Guangxi zedoary kind ball quality and the influence of blooming
(1) will be then the novel species ball be put in 2 ℃, 8 ℃, 13 ℃, 15 ℃, 17 ℃, 20 ℃, room temperature (21～32 ℃) respectively 40 days, screen best refrigerated storage temperature (referring to table 2).
Table 2 reserve temperature is to the influence of Guangxi zedoary kind ball germination rate and rotting rate
| Plant the |
2℃ | 8℃ | 13℃ | 15℃ | 17℃ | 20℃ | 21～32 ℃ (room temperature) |
| Germination rate (%) | 0 | 0 | 34 | 66 | 34 | 14 | 10 |
| Rotting rate (%) | 34 | 22 | 0 | 0 | 0 | 0 | 0 |
As known from Table 2, when reserve temperature was low to moderate 8 ℃, the germination rate of Guangxi zedoary kind ball was zero, and rotting rate is 22%, along with the reduction of reserve temperature, planted the ball rotting rate and raise gradually.When reserve temperature reached 13 ℃, it was 34% that the ball germination rate is planted in the storage back, and when reserve temperature was 15 ℃, germination rate was the highest, reached 66%, and along with the further rising of reserve temperature, the germination rate of planting ball descends.
(2) 2 grades of novel species balls are carried out the combined treatment (referring to table 3,4) of refrigerated storage temperature, germination temperature and time, place culturing room's water planting then.
The influence to Guangxi zedoary flowering rate (%) is handled in table 3 refrigeration and vernalization
As seen from Table 3, identical cold preservation time, the kind ball flowering rate of 30 ℃ of vernalization is higher than the kind ball of 25 ℃ of vernalization; Under identical cold preservation time and the germination temperature, germination time is long more, and flowering rate is high more.Under the identical vernalization condition, cold preservation time is long more, and flowering rate is high more.
The influence to Guangxi zedoary kind ball DTF is handled in table 4 refrigeration and vernalization
As seen from Table 4, under the identical germination temperature and germination time, cold preservation time is long more, and DTF is short more.Refrigerate 30～60 days Guangxi zedoary kind ball, 25 ℃ of vernalization 10 days, its DTF shortened to 55 days from 65 days; 25 ℃ of vernalization 20 days, its DTF shortened to 32 days from 62 days; 25 ℃ of vernalization 30 days, its DTF shortened to 30 days from 54 days; 30 ℃ of vernalization 30 days, its DTF shortened to 12 days from 25 days.Refrigeration and vernalization are handled greatly to have shifted to an earlier date and are planted the time that ball is bloomed.The Guangxi zedoary of cultivating in culturing room is about 8～10 days from the flower that begins to containing the flower required time, and under the winter of subtropical zone normal temperature, the Guangxi zedoary spray life-span can reach more than 50 days.Refrigerate 30～60 days, the kind ball full-bloom stage of 30 ℃ of vernalization is all during the Spring Festival, so can select the cultivation of welcoming spring of this method on producing for use.
The influence to Guangxi zedoary DTL is handled in table 5 refrigeration and vernalization
Table 5 as seen, 15 ℃ of refrigeration 10-60 days, 25 ℃ of vernalization 10 days, Guangxi zedoary kind ball DTL reduced to 51 days from 75 days, and all to plant balls all be to bloom behind the exhibition leaf earlier; Under the identical cold preservation time, along with 25 ℃ of germination times prolong, DTL shortens; When 25 ℃ of germination times extended to 30 days, Guangxi zedoary kind ball was bloomed earlier and is afterwards opened up leaf, and especially 30 days kind ball of 30 ℃ of vernalization is opened to full-bloom stage and just begun to open up leaf.
3. the influence that the Guangxi zedoary of different development stage is bloomed of the cultivation under the natural conditions
With 2 grades of new balls refrigerate in batches, after the vernalization, in culturing room, cultivate a period of time after, select the Guangxi zedoaries (referring to Fig. 1) of 7 developmental stage:
Stage 1: plant the ball 4 pure white sprout 1 of emerging, the long 3～5cm of bud;
Stage 2: sprout 1 most advanced and sophisticated commentaries on classics is red, the long 6～8cm of bud;
Stage 3: sprout 1 changes green, and the middle part begins to expand;
Stage 4: sprout 1 is expanded, and has just revealed aubergine inflorescence 3 in the green phyllary 2, but inflorescence is not when exposing;
Stage 5: aubergine inflorescence 3 exposes in bract 2, is about 0.5cm;
Stage 6: aubergine inflorescence 3 length reach 6～8cm;
Stage 7: first bract 2 of aubergine inflorescence 3 base portions opens;
The Guangxi zedoary in above 7 stages is taken out simultaneously, and being placed on the subtropical zone does not have the indoor of heating installation, and RH40～70% carries out water planting under the natural lighting condition.
The influence that water planting under table 6 natural conditions is bloomed to the different development stage Guangxi zedoary
| Stage | Spray height (cm) | Spray rugosity (cm) | Petal sheet number | DTB | DTS | Flowering rate (%) | Bract color and luster (rank) |
| 1 | - | - | - | - | - | 0 | - |
| 2 | 17.05 | 0.95 | 11 | 39 | 85 | 50 | 0 |
| 3 | 17.5 | 0.9 | 13 | 31 | 82 | 66 | 1 |
| 4 | 19.2 | 0.83 | 17 | 28 | 81 | 100 | 1 |
| 5 | 18.1 | 0.7 | 14 | 26 | 79 | 100 | 2 |
| 6 | 18.8 | 0.67 | 12 | 24 | 76 | 100 | 2 |
| 7 | 20.7 | 0.77 | 16 | 21 | 73 | 100 | 2 |
Table 6 as seen, the stage 1 places under the described natural conditions water planting not bloom; Stage 2,3 can bloom under described natural conditions, but flowering rate is respectively 50%, 66%, and the stage 4～7 is 100% in described natural conditions water planting flowering rate.DTB and DTS were successively decreased gradually by the stage 1～7, show that Guangxi zedoary kind ball grows late phase and place under the natural conditions water planting can significantly accelerate beginning flower and wilting process, and color and luster is more bright-coloured, but other spray height, rugosity, petal sheet number are not had obvious influence.If the developmental stage, can not guarantee that it blooms after placing under the natural conditions for a long time, even bloom, its bract color and luster is bright-coloured inadequately, influences ornamental value.Grow late phase and place that to carry out water planting under the described natural conditions favourable to its quality of blooming.
4. nutrient solution is to the growth effect of Guangxi zedoary
A, storage are handled: 47 kind balls are put into 13 ℃ of refrigerating chambers refrigerate processing 30 days.
B, vernalization handle: plant ball refrigeration and take out from refrigerating chamber after 30 days, place 30 ℃ indoor vernalization 10 days.
C, incubation growth: the kind ball after the vernalization is positioned in the container, and makes in kind of the ball fixed container with a spot of sand, at 30 ± 2 ℃, the 3000LUX light intensity is carried out under the condition of illumination water planting 24 hours every days and is cultivated with variable concentrations MS nutrient solution respectively after 3 days.
To plant ball and be divided into 3 groups, wherein handle 5 in 2 grades of kind balls for every group, 18 in 3 grades of kind balls, ball is 24 in 4 grades, and 3 groups of kind ball water plantings after 3 days, are soaked in clear water respectively; 1/4 times of MS nutrient solution; 1/8 times of MS nutrient solution; 1/16 times of MS nutrient solution; In 1/32 times of MS nutrient solution, be designated as T1 (contrast); T2; T3; T4; T5 tests other process and adds clear water.MS nutrient solution mother liquor is prepared according to known formulations.
Table 7 variable concentrations MS nutrient solution is to the influence of Guangxi zedoary rotting rate and flowering rate
Show that from table 7 each rotting rate of handling all is grade kind ball＜4,2 grades of kind ball＜3 grade kind balls, particularly the rotting rate of 4 grades of kind balls is very high, badly influences the normal growth and the survival rate of Guangxi zedoary kind ball.The developmental state of 2 grades of kind balls is good, and resistance is also better, so rotting rate is low.4 grades of kind balls a little less than, thereby be subjected to the influence of external condition easily and change causing rotting.Other processing is lower relatively and 1/8 times of MS nutrient solution is planted the rotting rate of ball in handling.
It is grade kind ball＞4,2 grades of kind ball＞3 grade kind balls substantially that table 7 is respectively handled the flowering rate rule, and the nutrition content in 2 grades of kind balls is the abundantest, can provide the growth of plant better and blooms.Wherein the flowering rate with 1/8 times of MS nutrient solution is the highest, and secondly 1/4 times of MS nutrient solution is handled.Rotting of kind of ball can not only be controlled and lower to explanation in the concentration that is fit to, also help it and bloom.
Data show throughout in 2 grades of kind balls of reason in the table 8, again flowering stage of handling with 1/8 times of MS nutrient solution and full-bloom stage all the earliest, the quality comprehensive of blooming also is the highest, wherein the effect handled of 1/4 times of MS nutrient solution is taken second place.The contrast effect is least desirable, and the flowering rate and the quality of blooming all are the poorest.Having illustrated that plant absorbs certain nutrition in necessary for growth could be better, more stably from the conversion reproductive growth of nourishing and growing.Therefore, draw from table 8, under the same terms, the influence of the reproductive growth of the Guangxi zedoary in 1/8 times of MS nutrient solution is best, and 1/4 takes second place.And other handles the reduction along with concentration, is all presenting downward trend from the quality of blooming such as flowering rate, flowering time.
The influence that table 8 variable concentrations MS nutrient solution is bloomed to 2 grades of kind balls of Guangxi zedoary
| Handle | Flowering rate (%) | Early flowering season (my god) | Full-bloom stage | Full-bloom stage spray height (CM) | Full-bloom stage spray rugosity (CM) | Petal sheet number | Bract color and luster (level) |
| T1 | 20 | 26 | 34 | 18 | 0.87 | 13b | 0 |
| T2 | 60 | 21 | 26 | 19.3 | 1.07 | 14 | 2 |
| T3 | 100 | 18 | 25 | 21.3 | 1.05 | 17 | 2 |
| T4 | 40 | 22 | 28 | 18.25 | 0.82 | 16 | 1 |
| T5 | 20 | 26 | 32 | 19.4 | 0.92 | 13 | 1 |
Demonstrate from table 9, bud degree of growing tall analysis after 4 weeks, plant height is followed successively by T3, T2, T4, T5, T1, T2 (i.e. 1/8 times of MS nutrient solution) is best to the effect of Guangxi zedoary kind ball normal growth, plant leaf bud growing height is the highest, the growing state of plant is the poorest in the control treatment, the plant growing way a little less than.Show that each concentration MS nutrient solution all has certain facilitation to the growth of Guangxi zedoary, can provide certain nutriment for the Guangxi zedoary growth, wherein the effect in each concentration is again with 1/8 times of MS nutrient solution the best, next is 1/4 times of MS nutrient solution, and 1/16 times of MS nutrient solution, 1/32 times of MS nutrient solution take second place, and the plant growing way is then the poorest in the contrast.
From table 9, can also learn, plant maintains a higher level substantially in the growth rate in preceding 4 weeks, and 1/8 times of MS nutrient solution and 1/4 times of MS nutrient solution leaf bud growth rate in handling early stage than comparatively fast, other processing is then increasing with more stable speed relatively.
The influence that table 9 variable concentrations MS nutrient solution is long to Guangxi zedoary kind spheroblast
Above experimental result shows that the present invention has following beneficial effect:
(1) the present invention can realize the forcing culture of Guangxi zedoary winter jasmine.Its flower bud differentiation and the process of blooming can be divided into 3 periods: 1. pass through 13～20 ℃ deepfreeze Guangxi zedoary kind ball, the dormancy of having broken Guangxi zedoary kind ball; 2. vernalization impels kind of ball to germinate under 25～30 ℃ high temperature; 3. in culturing room, the kind ball that germinates is carried out high-temperature cultivation and promote to expand, suppress it, improve its flowering rate to the reverse of nourishing and growing to flower bud development to sprout.Thereby make originally and will can during the Spring Festival, bloom and contain colored, reached the purpose at the florescence of having regulated and control Guangxi zedoary, for a kind of annual flower grass of top grade is provided the Spring Festival at the Guangxi zedoary that 4～June and 7～September bloom.
(2) in Guangxi zedoary winter jasmine cultivation and production, suit when acrial part is withered, to excavate kind of a ball at Guangxi zedoary, preferred 1～2 grade of kind ball (is planted bulb diameter 3～6cm), and preferred 15 ℃ of deepfreezes are after 30～60 days, 30 ℃ of vernalization 30 days, place 30 ℃ of culturing room's water to support again, can become high-quality winter jasmine hydroponic flowers to the 4th stage.
Description of drawings
Fig. 1 is the form schematic diagram of 7 developmental stage of Guangxi zedoary;
Wherein, 1: sprout; 2: bract; 3: the aubergine inflorescence; 4: plant ball.
Embodiment
By the following examples content of the present invention is further elaborated:
Embodiment one:
(1) gather the kind ball of Guangxi zedoary acrial part in October then after withered, wipe out withered, old root, clean up, screening, classification, selecting diameter is the kind ball of 4～6cm, is immersed in 10min in 800 times the tpn solution then, taking-up is dried, and stores;
(2) Guangxi zedoary kind ball is positioned in the culture plate, covers on kind of ball through sterilization and moistening loose sand, husky moistening degree is agglomerating to hold, and unclamping promptly looses is advisable; Then culture plate was put into 15 ℃, RH and is in 70% the refrigerating chamber refrigeration 60 days, during the refrigeration every day sooner or later each water spray once, indoor RH is 70% so that Keep cool;
(3) Guangxi zedoary kind ball is taken out from refrigerating chamber, plants ball and still be coated with sand, put in 30 ℃, the greenhouse of RH 〉=90% vernalization into 30 days, during the vernalization every day sooner or later each water spray once, to keep RH 〉=90% in the greenhouse;
(4) the kind ball after the vernalization is positioned over carries out water planting in the culturing room and cultivate, the water planting level is 3/7 of kind of ball height, and condition of culture is: 30 ± 2 ℃, RH 80 ± 10%, intensity of illumination 45 μ mol m
-2s
-1Periodicity of illumination 24 hours/day, being cultured to sprout expands, in the phyllary of green, just revealed the aubergine inflorescence, but when inflorescence does not expose, place then under the natural conditions in the Spring Festival of subtropical zone or artificial equal conditions under to carry out soil cultivation foster until blooming, promptly the environmental temperature of Pei Yanging is more than 10 ℃, humidity is more than 40%, light intensity 15 μ mol m
-2s
-1More than, periodicity of illumination is more than 9 hours/day.The flowering rate 83.3% of Guangxi zedoary, DTF12 days, DTL36 days, inflorescence length reached 30cm, and the whole florescence is about 35～45 days, and bract is that bright-coloured aubergine and color and luster are bright.
Embodiment two:
(1) gather the kind ball of Guangxi zedoary acrial part in October then after withered, flush away earth, cut off fibrous root, screening, classification, selecting diameter is the Guangxi zedoary kind ball of 4～6cm, then it is immersed in 10min in 800 times the tpn solution, taking-up is dried;
(2) Guangxi zedoary kind ball is positioned in the culture plate, covers through sterilization and moistening peat soil on kind of ball, the moistening degree of peat soil is agglomerating to hold, and unclamping promptly looses is advisable; Then culture plate was put into 15 ℃, RH and is in 80% the refrigerating chamber refrigeration 50 days, during the refrigeration every day sooner or later each water spray once, indoor RH is 80% so that Keep cool;
(3) will plant ball and from refrigerating chamber, take out, and plant ball and still be coated with peat soil, put in 30 ℃, the greenhouse of RH 〉=90% vernalization into 30 days, during the vernalization every day sooner or later each water spray once, to keep RH 〉=90% in the greenhouse;
(4) the kind ball after the vernalization is positioned over water planting cultivation in the culturing room, the water planting level is 1/3 of kind of ball height, and condition of culture is: 30 ± 2 ℃, RH 70 ± 10%, intensity of illumination 45 μ mol m
-2s
-1Periodicity of illumination 20h/d, being cultured to sprout expands, in the phyllary of green, just revealed the aubergine inflorescence, but when inflorescence does not expose, place then under the natural conditions in the Spring Festival of subtropical zone or artificial equal conditions under to carry out soil cultivation foster until blooming, promptly the environmental temperature of Pei Yanging is more than 10 ℃, humidity is more than 40%, light intensity 15 μ mol m
-2s
-1More than, periodicity of illumination is more than 9 hours/day.The flowering rate 92% of Guangxi zedoary, DTF18 days, DTL40 days, inflorescence length reached 28cm, and the whole florescence is about 35～45 days, and bract is that bright-coloured aubergine and color and luster are bright.
Embodiment three:
(1) gather the kind ball of Guangxi zedoary acrial part in November then after withered, flush away earth, cut off fibrous root, screening, classification, selecting diameter is the kind ball of 3～4cm, then it is immersed in 10min in 800 times the tpn solution, taking-up is dried;
(2) with in the Guangxi zedoary kind ball culture plate, on kind of ball, cover through sterilization and moistening perlite, perlitic moistening degree is agglomerating to hold, and unclamping promptly looses is advisable; Then culture plate was put into 15 ℃, RH and is in 90% the refrigerating chamber refrigeration 40 days, during the refrigeration every day sooner or later each water spray once, indoor RH is 90% so that Keep cool;
(3) will plant ball and from refrigerating chamber, take out, and plant ball and still be coated with perlite, put in 30 ℃, the greenhouse of RH 〉=90% vernalization into 30 days, during the vernalization every day sooner or later each water spray once, to keep RH 〉=90% in the greenhouse;
(4) the kind ball after the vernalization is positioned over water planting cultivation in the culturing room, the water planting level is 1/2 of kind of ball height, and condition of culture is: 30 ± 2 ℃, RH 70 ± 10%, intensity of illumination 45 μ mol m
-2s
-1Periodicity of illumination 16h/d, being cultured to sprout expands, in the phyllary of green, just revealed the aubergine inflorescence, but when inflorescence does not expose, place then under the natural conditions in the Spring Festival of subtropical zone or artificial equal conditions under to carry out soil cultivation foster until blooming, promptly the environmental temperature of Pei Yanging is more than 10 ℃, humidity is more than 40%, light intensity 15 μ mol m
-2s
-1More than, periodicity of illumination is more than 9 hours/day.The flowering rate 56% of Guangxi zedoary, DTF24 days, DTL40 days, inflorescence length reached 30cm, and the whole florescence is about 35～45 days, and bract is that bright-coloured aubergine and color and luster are bright.
Embodiment four:
(1) gather the kind ball of Guangxi zedoary acrial part in November then after withered, flush away earth, cut off fibrous root, screening, classification, selecting diameter is the kind ball of 3～4cm, then it is soaked with 800 times tpn solution in 10min, taking-up is dried;
(2) with in the Guangxi zedoary kind ball culture plate, on kind of ball, cover clean and moistening sand through sterilization, husky moistening degree is agglomerating to hold, and unclamping promptly looses is advisable; Then culture plate was put into 15 ℃, RH and is in 95% the refrigerating chamber refrigeration 30 days, during the refrigeration every day sooner or later each water spray once, indoor RH is 95% so that Keep cool;
(3) will plant ball and from refrigerating chamber, take out, and plant ball and still be coated with perlite, put in 30 ℃, the greenhouse of RH 〉=90% vernalization into 30 days, during the vernalization every day sooner or later each water spray once, to keep RH 〉=90% in the greenhouse;
(4) the kind ball after the vernalization is taken out, be positioned over water planting cultivation in the culturing room, the water planting level is 1/3 of kind of ball height, and condition of culture is: 30 ± 2 ℃, RH 70 ± 10%, intensity of illumination 45 μ mol m
-2s
-1Periodicity of illumination 24h/d, be cultured to sprout and expand, in the phyllary of green, just revealed the aubergine inflorescence, but inflorescence is not when exposing, place then under the natural conditions in the Spring Festival of subtropical zone or under the artificial equal conditions and carry out water planting until blooming, the kind ball that is about to form bud is put into water receptacle, and the water planting level is 1/2 of kind of ball height, and promptly the environmental temperature of Pei Yanging is more than 10 ℃, humidity is more than 40%, light intensity 15 μ mol m
-2s
-1More than, periodicity of illumination is more than 9 hours/day.The flowering rate 46% of Guangxi zedoary, DTF25 days, DTL48 days, inflorescence length reached 32cm, and the whole florescence is about 35～45 days, and bract is that bright-coloured aubergine and color and luster are bright.
Embodiment five:
(1) gather the kind ball of Guangxi zedoary acrial part in November then after withered, flush away earth, cut off fibrous root, screening, classification, selecting diameter is the kind ball of 3～4cm, then it is soaked with 800 times tpn solution in 10min, taking-up is dried;
(2) with in the Guangxi zedoary kind ball culture plate, on kind of ball, cover clean and moistening sand through sterilization, husky moistening degree is agglomerating to hold, and unclamping promptly looses is advisable; Then culture plate was put into 15 ℃, RH and is in 85% the refrigerating chamber refrigeration 60 days, during the refrigeration every day sooner or later each water spray once, indoor RH is 85% so that Keep cool;
(3) will plant ball and from refrigerating chamber, take out, and plant ball and still be coated with perlite, put in 25 ℃, the greenhouse of RH 〉=90% vernalization into 30 days, during the vernalization every day sooner or later each water spray once, to keep RH 〉=90% in the greenhouse;
(4) the kind ball after the vernalization is taken out, be positioned in the culturing room and cultivate, the water planting level is 1/3～1/2 of kind of ball height, and condition of culture is: 30 ± 2 ℃, RH 70 ± 10%, intensity of illumination 45 μ mol m
-2s
-1According to cycle 24h/d, be cultured to sprout and expand, in the phyllary of green, just revealed the aubergine inflorescence, but inflorescence is not when exposing, place then under the natural conditions in the Spring Festival of subtropical zone or artificial equal conditions under carry out water planting and cultivate until blooming, the kind ball that is about to form bud is put into water receptacle, and the water planting level is 1/3 of kind of ball height, and promptly the environmental temperature of Pei Yanging is more than 10 ℃, humidity is more than 40%, light intensity 15 μ mol m
-2s
-1More than, periodicity of illumination is more than 9 hours/day.The flowering rate 34% of Guangxi zedoary, DTF30 days, DTL38 days, inflorescence length reached 32cm, and the whole florescence is about 35～45 days, and bract is that bright-coloured aubergine and color and luster are bright.
Embodiment six:
(1) gather the kind ball of Guangxi zedoary acrial part in November then after withered, flush away earth, cut off fibrous root, screening, classification, selecting diameter is the kind ball of 3～4cm, then it is soaked with 800 times tpn solution in 10min, taking-up is dried;
(2) with in the Guangxi zedoary kind ball culture plate, on kind of ball, cover clean and moistening sand through sterilization, husky moistening degree is agglomerating to hold, and unclamping promptly looses is advisable; Then culture plate was put into 15 ℃, RH and is in 75% the refrigerating chamber refrigeration 50 days, during the refrigeration every day sooner or later each water spray once, indoor RH is 75% so that Keep cool;
(3) will plant ball and from refrigerating chamber, take out, and plant ball and still be coated with perlite, put in 25 ℃, the greenhouse of RH 〉=90% vernalization into 30 days, during the vernalization every day sooner or later each water spray once, to keep RH 〉=90% in the greenhouse;
(4) the kind ball after the vernalization is taken out, be positioned over water planting cultivation in the culturing room, the water planting level is 1/3 of kind of ball height, and condition of culture is: 30 ± 2 ℃, RH 70 ± 10%, intensity of illumination 45 μ mol m
-2s
-1, periodicity of illumination 24h/d; Added 1/8 times of MS nutrient solution every 10 days; Being cultured to sprout expands, in the phyllary of green, just revealed the aubergine inflorescence, but when inflorescence does not expose, place then under the natural conditions in the Spring Festival of subtropical zone or artificial equal conditions under to carry out soil cultivation foster until blooming, promptly the environmental temperature of Pei Yanging is more than 10 ℃, humidity is more than 40%, light intensity 15 μ mol m
-2s
-1More than, periodicity of illumination is more than 9 hours/day.And every 1/8 times of MS nutrient solution of interpolation in 10 days.The flowering rate 58% of Guangxi zedoary, DTF38 days, DTL49 days, the whole florescence was about 35～45 days, bract is that bright-coloured aubergine and color and luster are bright.
Embodiment seven:
(1) gather the kind ball of Guangxi zedoary acrial part in November then after withered, flush away earth, cut off fibrous root, screening, classification, selecting diameter is the kind ball of 3～4cm, then it is soaked with 800 times tpn solution in 10min, taking-up is dried;
(2) with in the Guangxi zedoary kind ball culture plate, on kind of ball, cover clean and moistening sand through sterilization, husky moistening degree is agglomerating to hold, and unclamping promptly looses is advisable; Then culture plate was put into 15 ℃, RH and is in 80～90% the refrigerating chamber refrigeration 40 days, during the refrigeration every day sooner or later each water spray once, indoor RH is 80～90% so that Keep cool;
(3) will plant ball and from refrigerating chamber, take out, and plant ball and still be coated with perlite, put in 25 ℃, the greenhouse of RH 〉=90% vernalization into 30 days, during the vernalization every day sooner or later each water spray once, to keep RH 〉=90% in the greenhouse;
(4) the kind ball after the vernalization is taken out, be positioned over water planting cultivation in the culturing room, the water planting level is 3/8 of kind of ball height, and condition of culture is: 30 ± 2 ℃, RH 70 ± 10%, intensity of illumination 45 μ mol m
-2s
-1, periodicity of illumination 24h/d; Added 1/32 times of MS nutrient solution every 10 days; Being cultured to sprout expands, in the phyllary of green, just revealed the aubergine inflorescence, but when inflorescence does not expose, place then under the natural conditions in the Spring Festival of subtropical zone or artificial equal conditions under to carry out soil cultivation foster until blooming, promptly the environmental temperature of Pei Yanging is more than 10 ℃, humidity is more than 40%, light intensity 15 μ mol m
-2s
-1More than, periodicity of illumination is more than 9 hours/day.And every 1/32 times of MS nutrient solution of interpolation in 10 days.The flowering rate 16% of Guangxi zedoary, DTF42 days, DTL50 days, inflorescence length reached 30cm, and the whole florescence is about 35～45 days, and bract is that bright-coloured aubergine and color and luster are bright.
Embodiment eight:
(1) gather the kind ball of Guangxi zedoary acrial part in November then after withered, flush away earth, cut off fibrous root, screening, classification, selecting diameter is the kind ball of 3～4cm, then it is soaked with 800 times tpn solution in 10min, taking-up is dried;
(2) with in the Guangxi zedoary kind ball culture plate, on kind of ball, cover clean and moistening sand through sterilization, husky moistening degree is agglomerating to hold, and unclamping promptly looses is advisable; Then culture plate was put into 15 ℃, RH and is in 70～80% the refrigerating chamber refrigeration 60 days, during the refrigeration every day sooner or later each water spray once, indoor RH is 70～80% so that Keep cool;
(3) will plant ball and from refrigerating chamber, take out, and plant ball and still be coated with perlite, put in 25 ℃, the greenhouse of RH 〉=90% vernalization into 20 days, during the vernalization every day sooner or later each water spray once, to keep RH 〉=90% in the greenhouse;
(4) the kind ball after the vernalization is taken out, be positioned over water planting cultivation in the culturing room, the water planting level is 3/7 of kind of ball height, and condition of culture is: 30 ± 2 ℃, RH 70 ± 10%, intensity of illumination 45 μ mol m
-2s
-1, periodicity of illumination 24h/d; Added 1/16 times of MS nutrient solution every 10 days; Being cultured to sprout expands, in the phyllary of green, just revealed the aubergine inflorescence, but when inflorescence does not expose, place then under the natural conditions in the Spring Festival of subtropical zone or artificial equal conditions under carry out water planting and cultivate until blooming, the kind ball that is about to form bud is put into water receptacle, and the water planting level is 1/3 of kind of ball height, and promptly the environmental temperature of Pei Yanging is more than 10 ℃, humidity is more than 40%, light intensity 15 μ mol m
-2s
-1More than, periodicity of illumination is more than 9 hours/day.And every 1/16 times of MS nutrient solution of interpolation in 10 days.The flowering rate 34% of Guangxi zedoary, DTF32 days, DTL40 days, inflorescence length reached 30cm, and the whole florescence is about 35～45 days, and bract is that bright-coloured aubergine and color and luster are bright.
Embodiment nine:
(1) gather the kind ball of Guangxi zedoary acrial part in November then after withered, flush away earth, cut off fibrous root, screening, classification, selecting diameter is the kind ball of 3～4cm, then it is soaked with 800 times tpn solution in 10min, taking-up is dried;
(2) with in the Guangxi zedoary kind ball culture plate, on kind of ball, cover clean and moistening sand through sterilization, husky moistening degree is agglomerating to hold, and unclamping promptly looses is advisable; Then culture plate was put into 15 ℃, RH and is in 80～90% the refrigerating chamber refrigeration 60 days, during the refrigeration every day sooner or later each water spray once, indoor RH is 80～90% so that Keep cool;
(3) will plant ball and from refrigerating chamber, take out, and plant ball and still be coated with perlite, put in 25 ℃, the greenhouse of RH 〉=90% vernalization into 10 days, during the vernalization every day sooner or later each water spray once, to keep RH 〉=90% in the greenhouse;
(4) the kind ball after the vernalization is taken out, be positioned over water planting cultivation in the culturing room, the water planting level is 1/3 of kind of ball height, and condition of culture is: 30 ± 2 ℃, RH 70 ± 10%, intensity of illumination 45 μ mol m
-2s
-1, periodicity of illumination 24h/d; Added 1/4 times of MS nutrient solution every 10 days; Being cultured to sprout expands, in the phyllary of green, just revealed the aubergine inflorescence, but when inflorescence does not expose, place then under the natural conditions in the Spring Festival of subtropical zone or artificial equal conditions under carry out water planting and cultivate until blooming, the kind ball that is about to form bud is put into water receptacle, and the water planting level is 3/8 of kind of ball height, and promptly the environmental temperature of Pei Yanging is more than 10 ℃, humidity is more than 40%, light intensity 15 μ mol m
-2s
-1More than, periodicity of illumination is more than 9 hours/day.And every 1/4 times of MS nutrient solution of interpolation in 10 days.The flowering rate 16% of Guangxi zedoary, DTF55 days, DTL51 days, inflorescence length reached 35cm, and the whole florescence is about 35～45 days, and bract is that bright-coloured aubergine and color and luster are bright.
Embodiment ten:
(1) gather the kind ball of Guangxi zedoary acrial part in November then after withered, flush away earth, cut off fibrous root, screening, classification, selecting diameter is the kind ball of 3～4cm, then it is soaked with 800 times tpn solution in 10min, taking-up is dried;
(2) with in the Guangxi zedoary kind ball culture plate, on kind of ball, cover clean and moistening sand through sterilization, husky moistening degree is agglomerating to hold, and unclamping promptly looses is advisable; Then culture plate was put into 15 ℃, RH and is in 80～90% the refrigerating chamber refrigeration 50 days, during the refrigeration every day sooner or later each water spray once, indoor RH is 80～90% so that Keep cool;
(3) will plant ball and from refrigerating chamber, take out, and plant ball and still be coated with perlite, put in 25 ℃, the greenhouse of RH 〉=90% vernalization into 10 days, during the vernalization every day sooner or later each water spray once, to keep RH 〉=90% in the greenhouse;
(4) the kind ball after the vernalization is taken out, be positioned over water planting cultivation in the culturing room, the water planting level is 1/3 of kind of ball height, and condition of culture is: 30 ± 2 ℃, RH 70 ± 10%, intensity of illumination 45 μ mol m
-2s
-1, periodicity of illumination 24h/d; Added 1/4 times of MS nutrient solution every 10 days; Being cultured to sprout expands, in the phyllary of green, just revealed the aubergine inflorescence, but when inflorescence does not expose, place then under the natural conditions in the Spring Festival of subtropical zone or artificial equal conditions under to carry out soil cultivation foster until blooming, promptly the environmental temperature of Pei Yanging is more than 10 ℃, humidity is more than 40%, light intensity 15 μ mol m
-2s
-1More than, periodicity of illumination is more than 9 hours/day.And every 1/4 times of MS nutrient solution of interpolation in 10 days.The flowering rate 16% of Guangxi zedoary, DTF58 days, DTL56 days, inflorescence length reached 32cm, and the whole florescence is about 35～45 days, and bract is that bright-coloured aubergine and color and luster are bright.
The present invention can summarize with other the concrete form without prejudice to spirit of the present invention or principal character.Above-mentioned embodiment of the present invention all can only be thought explanation of the present invention rather than restriction, therefore in implication suitable with claims of the present invention and any change in the scope, all should think to be included in the scope of claims.
Claims (10)
1, a kind of Guangxi zedoary florescence control method is characterized in that, it may further comprise the steps:
(1) processing of kind ball: collection, screening, classification, cleaning and sterilizing Guangxi zedoary seedballs, dry then;
(2) refrigeration: Guangxi zedoary kind ball is placed on 13～20 ℃, and relative moisture is refrigeration 30～60 days in 70～95% the refrigerating chamber;
(3) vernalization: will plant ball and take out from refrigerating chamber, and put 25～30 ℃ into, vernalization is 10～30 days in the greenhouse of relative moisture 〉=90%;
(4) Hua cultivation: the kind ball after the vernalization is positioned over is cultured to sprout in the culturing room and expands, in the phyllary of green, reveal the aubergine inflorescence, but inflorescence can place the kind ball that forms bud under the natural conditions and cultivate when not exposing.
2, Guangxi zedoary florescence control method according to claim 1 is characterized in that, the kind ball in the step (4) after the vernalization is positioned over the training method of cultivating in the culturing room and adopts water planting to cultivate; At this moment, the condition of described culturing room is: temperature is 25～32 ℃, and relative moisture is 60～90%, intensity of illumination 45 μ mol m
-2s
-1
3, Guangxi zedoary florescence control method according to claim 1 and 2 is characterized in that, the temperature of refrigeration is 13～17 ℃ in the step (2), and cold preservation time is 40～60 days.
4, Guangxi zedoary florescence control method according to claim 3 is characterized in that, the temperature of refrigeration is 14～17 ℃ in the step (2), and cold preservation time is 50～60 days.
5, Guangxi zedoary florescence control method according to claim 4 is characterized in that, all covers clean and loose culture medium in step (2) refrigeration and step (3) vernalization on kind of ball; Spray water twice every day during refrigeration or the vernalization.
6, Guangxi zedoary florescence control method according to claim 5 is characterized in that, in step (4), when the kind ball is successively cultivated, adds nutrient solution every 8～12 days under culturing room's cultivation and natural conditions.
7, Guangxi zedoary florescence control method according to claim 6 is characterized in that, described nutrient solution is the MS nutrient solution.
8, Guangxi zedoary florescence control method according to claim 7 is characterized in that, the concentration of described MS nutrient solution is 1/4～1/32 times of MS nutrient solution mother liquid concentration.
9, Guangxi zedoary florescence control method according to claim 8 is characterized in that, described kind of ball of step (1) selected new then ball, and is at the kind ball of gathering in October and November then after the Guangxi zedoary acrial part is withered.
10, Guangxi zedoary florescence control method according to claim 9 is characterized in that, it is 2～6cm kind ball that described kind of ball adopts diameter.
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| CN101828479A (en) * | 2010-06-01 | 2010-09-15 | 江苏省农业科学院 | Method for regulating and controlling flowering period of potted flowering peach |
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| CN101828479A (en) * | 2010-06-01 | 2010-09-15 | 江苏省农业科学院 | Method for regulating and controlling flowering period of potted flowering peach |
| CN103597965A (en) * | 2013-10-22 | 2014-02-26 | 仲恺农业工程学院 | Method for long-term storage of Curcuma kwangsiensis 'Ma Nao' seed-balls |
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| CN104186145A (en) * | 2014-08-13 | 2014-12-10 | 浙江省萧山棉麻研究所 | Artificial control promotion cultivation method for solomen curcuma longa in target flowering phase |
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| CN104285537A (en) * | 2014-09-19 | 2015-01-21 | 广州普邦园林股份有限公司 | Method for increasing flowering rate of rootstalks of Hongyun curcuma zedoary cultured in water |
| CN104285537B (en) * | 2014-09-19 | 2016-04-13 | 广州普邦园林股份有限公司 | A kind ofly improve the method that water supports red cloud curcuma zedoary rhizome flowering rate |
| CN104705061A (en) * | 2015-03-24 | 2015-06-17 | 广州华苑园艺有限公司 | Planting method for high-quality curcuma kwangsinesis |
| CN104705061B (en) * | 2015-03-24 | 2017-03-01 | 广州华苑园林股份有限公司 | A kind of cultural method of Guangxi zedoary |
| CN105993482A (en) * | 2016-05-24 | 2016-10-12 | 田东县浙缘农业科技有限公司 | Interplanting method for rhizoma zedoariae and peanuts |
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Address after: 510000 Guangdong, Guangzhou, Zhongshan, No. 1, Yuexiu District Road, No. 34, No. 3 Patentee after: HUAYUAN LANDSCAPE ARCHITECTURE CO., LTD. Address before: 510000 Guangdong, Guangzhou, Zhongshan, No. 1, Yuexiu District Road, No. 34, No. 3 Patentee before: Guangzhou Huayuan Landscaping Co.,Ltd. |