A kind ofly improve the method that water supports red cloud curcuma zedoary rhizome flowering rate
Technical field
What the present invention relates to is a kind ofly improve the method that water supports red cloud curcuma zedoary rhizome flowering rate.
Background technology
Red cloud curcuma zedoary is Zingiber (Zingberaceae) turmeric (Curcuma) perennial root herbaceos perennial, be by the Nankunshan curcuma zedoary of wild stocks on Mt. Nankun of Guangdong Province (
curcumanankunshanensisn.Liu, X.B.Ye & J.Chen) as the new varieties of parent through domestication and improvement gained, because the form of its inflorescence top bract and color exactly like the red cloud of blade and gain the name.
Red cloud curcuma zedoary growing way is vigorous, and without branch, strain shape is compact, first spends posterior lobe, and the wide lanceolar of the oval shape of blade is to narrow lanceolar, and length of blade can reach more than 60cm, and width also can reach about 16cm, often has dun lilac band, closely without hair, blade back is close by pubescence in the middle part of blade face.Plant height is about 0.6-0.8m, strain width 40-55cm, inflorescence is healthy and strong, and inflorescence top bract aubergine is to pink, and middle and lower part bract is green to the first end band kermesinus of green white, oval bract quality wax is as jade, with imbricate arrangement, whole inflorescence shape, as pagoda, is bloomed gorgeous and the duration is long, the inflorescence life-span is about 30 days, has sight.As illustrated in fig. 1 and 2, the rhizome of red cloud curcuma zedoary is sturdy, main root stem is oval, taupe, top is comparatively mild, and usually long on main root stem 1 have lateral rhizome 2, root tuber of aromatic turmeric 5, old root 4 and fibrous root 3, and its main root stem 1 has the lateral rhizome 2 of side more flourishing usually, size dimension is slightly less than main root stem 1, and the lateral rhizome 2 of opposite side is then not obvious.
Red cloud curcuma zedoary this be earth culture plant, its nature florescence is the 4-6 month and the 7-9 month, and spike spring is from rhizome extraction, and autumn extracts out from leaf sheath, is about 30-40cm, and diameter is about 7-10cm.According to the general characteristic of curcuma zedoary, it also can carry out water and supports in theory, and the water red cloud curcuma zedoary of supporting is preferably does indoor appreciation flowers.In addition, the Spring Festival is middle national expenditures flower amount maximum season, can really the flowers supported of water are except the hyacinth of import and the narcissus in ZhangZhou in annual flower city, and what can bloom under water supports condition is few.If red cloud curcuma zedoary can be done water to support, and florescence control is to the Spring Festival, then the high-grade water of the one of red cloud curcuma zedoary can be made to become should save very much, doing something for the occasion supports annual flower grass, and this develops Chinese Resources of Wild and advances new flowers commercialization significant.
But, the present inventor red cloud curcuma zedoary is carried out water support attempt time, but find that the red cloud curcuma zedoary flowering rate that water is supported is extremely low, the red cloud curcuma zedoary that the 1st year water is supported all is nourished and grown substantially, all grows healthy and strong blade and without inflorescence.The florescence controls such as the plant Guangxi zedoary that the present inventor's reference in the 2nd year and red cloud curcuma zedoary belong to together and Nan Ling curcuma zedoary and water breeding method carry out water to red cloud curcuma zedoary rhizome and support, remain and only have minority to bloom, flowering rate is 5 ~ 10%, it is still extremely low flowering rate, than water support Guangxi zedoary and Nan Ling curcuma zedoary flowering rate (85%) much lower, also more much lower than the flowering naturally rate (65%) during red cloud curcuma zedoary field production, therefore, how to improve flowering rate that water supports red cloud curcuma zedoary and become the primary problem that its success applies, otherwise, aforesaid meaning of supporting annual flower grass as high-grade water about red cloud curcuma zedoary will become empty talk, meaningless.
Summary of the invention
The object of this invention is to provide and a kind ofly improve the method that water supports red cloud curcuma zedoary rhizome flowering rate.
The object of the invention is to be realized by following technical measures: a kind ofly improve the method that water supports red cloud curcuma zedoary rhizome flowering rate, its step is as follows:
(1) red cloud curcuma zedoary rhizome is gathered;
(2) root tuber of aromatic turmeric on red cloud curcuma zedoary main root stem, old root and fibrous root are removed, main root stem retains lateral rhizome;
(3) rhizome of step (2) gained is carried out cleaning, sterilize and drying;
(4) refrigerated by the rhizome of step (3) gained, refrigerated storage temperature is within the scope of 10 ~ 13 DEG C, and cold preservation time is in 30 ~ 60 days;
(5) rhizome after step (4) being refrigerated carries out vernalization, treats that bud grows to 4 ~ 5cm, and taking-up is carried out water and supported;
(6) water is supported to blooming.
Inventor finds when cultivating, and when processing red cloud curcuma zedoary rhizome, removes old root and fibrous root, retains lateral rhizome (shown in Figure 2), can significantly improve the flowering rate of red cloud curcuma zedoary rhizome.And if by traditional water breeding method only adopt red cloud curcuma zedoary main root stem carry out water support, then its flowering rate is but not high.So when doing rhizome process, the lateral rhizome of necessary complete reservation red cloud curcuma zedoary prosperity, after cleaning, sterilize and drying, then refrigerate, cold preservation time should not lower than 30 days or more than 60 days, otherwise all can cause flowering rate to reduce.
In described step (2), main root stem retains the lateral rhizome of more than 50%, preferably retain complete lateral rhizome, namely retain all lateral rhizomes.
The present invention can do following improvement: rhizome described in step (1) selects new ball then, and be gather after acrial part is withered and yellow for 11 ~ December then red cloud curcuma zedoary acrial part wither after rhizome.Can, by florescence control to the Spring Festival, to realize winter jasmine cultivation, be the flowers that flower market in the Spring Festival provides ornamental value higher.
The present invention can also do following improvement: because the germination rate of red cloud curcuma zedoary and the content height of protein have obvious correlation, and rhizome is larger, the protein content in its body is higher, therefore, rhizome of the present invention selects diameter to be 5.5 ~ 8.0cm rhizome, can obtain good germination rate.
In step of the present invention (5), germination temperature is 28 ~ 30 DEG C, and the time is 30 ~ 40 days.
The present invention can do following improvement further: during the vernalization of step (5), carry out drought stress, by the rhizome clear water taken out from freezer, running water (water under room temperature) can be selected to soak 20 ~ 40 minutes after, cover with the moistening fine sand of water content about 55%, 1 water is sprayed after within 10 days, waiting for fine sand bone dry afterwards, to squirt completely in the middle part of rhizome from fine sand surface, then no longer moisture content is given, until take out red cloud curcuma zedoary rhizome from germinating cabinet, Flower induction breaks up to adopt such drought stress to promote.
The water time of supporting in step of the present invention (6) is 10 ~ 25 days, and temperature is in the scope of 28 ~ 30 DEG C, and intensity of illumination is 2000 ~ 3000LUX, relative air humidity (RH) preferably 70 ~ 80%.Because hot and humid and high light accelerates its speed of blooming, allow its opening as early as possible, to meet the needs of blooming the Spring Festival.
One of embodiment that the present invention recommends is as follows, and in described step (6), when water supports beginning, topped up with water in culture vessel, induces new root to grow bud point from rhizome, to maintain growth and the evaporation needs of 1 week; After cultivating 1 week, keep container completely anhydrous 2 ~ 3 days, during container overdrying, atomized water spray increases humidity; Add a small amount of moisture maintenance rhizome afterwards slowly to grow, continue about 3 ~ 4 days; Wait for that the bud mutation of red cloud curcuma zedoary becomes red, and rhizome dry tack free is to when omiting dehydration, recovers normal water and support management.Red cloud curcuma zedoary changes by nourishing and growing to reproductive growth to adopt such drought stress to promote, Accelerate bloom.A small amount of moisture of the present invention refers to that the added water yield makes water level about arrive 1/5 place of rhizome height; The described slightly dehydration that is dried to refers to that rhizome surface is partially white, the slightly shrinkage of rhizome epidermis; Described normal water is supported management and is referred to that the moisture content added in container remains on 1/2 place of rhizome height.
The present invention can do following improvement: can survive for a long time at low temperatures to protect rhizome; and when vernalization for rhizome provides sufficient moisture, in step (4) refrigeration and step (5) vernalization, all on rhizome, cover clean culture medium.
Described culture medium can be through the one in the sand of sterilization, peat soil and perlite, also can be the mixing of in them any two or three.
Further, support period nutrient availability liquid replacement moisture in vernalization and water, better quality can be obtained.
One of embodiment that the present invention recommends is: nutrient solution used during the vernalization of step (5) and the water of step (6) are supported is recommended to select MS nutrient solution, it is a kind of low conductivity nutrient solution, containing macroelement and trace elements such as the nitrogen needed for plant, phosphorus, potassium, zinc, copper, molybdenum, iron.Because the Growth and anthesis of nutrient solution to red cloud curcuma zedoary of variable concentrations has a significant impact, concentration is too low, affects the normal absorption of red cloud curcuma zedoary, even may produce concentration difference and cause the dehydration of red cloud curcuma zedoary; Then can Developing restraint during high concentration, even make Plant death.Therefore, the concentration of described MS nutrient solution is 1/4 ~ 1/32 times of MS nutrient solution mother liquid concentration, and preferred concentration is 1/4 ~ 1/16 times of MS nutrient solution mother liquid concentration.Described MS nutrient solution mother liquor is according to existing formulated.
it is as follows that red cloud curcuma zedoary water of the present invention supports the method beneficial effect of blooming:
The experiment of lower mask body and experimental data can make the present invention of those skilled in the art of the present technique's comprehend, but do not limit the present invention in any way:
As seen from Table 1, at different temperature, there is different the early flowering season to red cloud curcuma zedoary rhizome, and along with the rising of temperature, and the early flowering season presents the trend of shortening.Particularly compared with when 20 DEG C, when temperature brings up to 24 DEG C and 28 DEG C, the early flowering season shortens 11 days and 18 days respectively.Can find out in observation statistic processes, under identical cultivation temperature, control water whether also the early flowering season is not made significant difference.
As seen from Table 2, red cloud curcuma zedoary rhizome is supported in process at water, and temperature and control water all have a certain impact to its flowering rate.Along with the rising of temperature, flowering rate also presents the trend of rising, and 24 DEG C time, the rhizome flowering rate giving to control water reaches 70%, and when 28 DEG C, flowering rate reaches 84%.At the same temperature, whether significantly on the impact of flowering rate water is controlled.20 DEG C time, give to control the rhizome flowering rate of water than not controlling the high by 18% of water, the rhizome flowering rate of 24 DEG C of time control water also exceeds 12% than what do not control water, and during to 28 DEG C, gap between the two has 8%.Cultivation temperature is lower, and the raising of control water to its flowering rate is more obvious, and along with the rising of temperature, control water reduces the impact that flowering rate improves.Therefore, can find out, concerning red cloud curcuma zedoary rhizome, control water and high temperature are the effective ways improving its flowering rate.
As seen from Table 3, red cloud curcuma zedoary is supported in process at water, and temperature and control water had a certain impact to its spray life-span.When giving control water equally, the rhizome spray life-span of cultivating at 20 DEG C reaches 55 days, foreshortens to 49 days, only have 38 days to when 28 DEG C when 24 DEG C.And at that same temperature, giving to control the rhizome of water compared with the rhizome spray life-span not controlling water, number of days reduces to some extent, and front and back do not differ 2 ~ 3 days not etc.Visible, under the condition that control regimen condition is identical, environmental temperature is higher, and the spray life-span of red cloud curcuma zedoary is shorter.And when temperature is identical, the rhizome giving to control water is shorter than the rhizome spray life-span not controlling water.
As seen from Table 4, support in process at red cloud curcuma zedoary at water, different temperatures and the length of control water to its spray do not make significant difference, and wherein drought stress group inflorescence length is slightly not longer than drought stress group.
table 5 is visible, do not controlling under water condition, the red cloud curcuma zedoary rhizome that lateral rhizome all retains water support period flowering rate up to 75%, and the red cloud curcuma zedoary rhizome flowering rate during water is supported retaining 50% lateral rhizome is 40%, the processed group flowering rate of all wiping out when lateral rhizome is only 20%, significant difference between 3 processed group.
As shown in Table 6, during the vernalization of red cloud curcuma zedoary, carry out control water result to it is that process A flowering rate is the highest, reaches 62%, namely within the 1st day, gives adequate water in vernalization, add water 1 time, after this do not feed water after 7 days; Process C rhizome flowering rate is taken second place, and is 58%, namely within every 5 days, adds water 1 time; Flowering rate is followed successively by D, B, E from high to low, and namely within every 10 days, adding water adds water for 1 time, every 3 days adds adequate water in 1 time, the 1st day, does not after this add water.With a little visible, during vernalization, control water can affect the flowering rate of red cloud curcuma zedoary rhizome, and suitable control water can improve its flowering rate.
above experimental result shows that the present invention has following beneficial effect:
(1) the invention solves water and support the extremely low problem of the flowering rate of red cloud curcuma zedoary rhizome, flowering rate reaches as high as 84%, thus successfully to plant being originally only suitable for soil and potted plant mode is planted the red cloud curcuma zedoary of blooming and carried out that water is foster blooms, the water providing a kind of top grade for flowers market is grown flower grass, both further expand the scope of application of red cloud curcuma zedoary, turn improve its commodity value and economic benefit.
(2) the present invention can also realize the adjustment at red cloud curcuma zedoary florescence further, enable originally to bloom at Spring Festival at the red cloud curcuma zedoary of blooming in 5 ~ June and 7 ~ September, make it to become should save very much, a kind of year night water of top grade of doing something for the occasion grows flower grass, this develops Chinese Resources of Wild and advances new flowers commercialization significant.
(3) the present invention realizes red cloud curcuma zedoary and cultivates in the clear water of cleaning, both can the inflorescence of enjoy beautiful, and can watch again the whole growth and development process of rhizome, its value of viewing and admiring is not only attractive in appearance, also with certain entertaining.
(4) water of the present invention supports the impact that red cloud curcuma zedoary farthest can also reduce cultivation matrix, smell that the generation of the damage by disease and insect such as some microorganisms in cultivation matrix and some matrix produces can be reduced on the impact of people, can the growth of direct viewing root, ornamental value is higher.
Accompanying drawing explanation
Fig. 1 is the full rhizome schematic diagram of red cloud curcuma zedoary.
Fig. 2 is the main root stem schematic diagram that red cloud curcuma zedoary remains lateral rhizome.
Embodiment
A small amount of moisture of the present invention refers to that the added water yield makes water level about arrive 1/5 place of rhizome height.The described slightly dehydration that is dried to refers to that rhizome surface is partially white, the slightly shrinkage of rhizome epidermis.Described normal water is supported management and is referred to that the moisture content added in container remains on 1/2 place of rhizome height.
embodiment one: water is supported
gather and pretreatment:gather gather November then red cloud curcuma zedoary acrial part wither after red cloud curcuma zedoary rhizome (see Fig. 1), remove root tuber of aromatic turmeric and old root and fibrous root, retain complete lateral rhizome (see Fig. 2), lateral rhizome is about 8.0 ~ 10.0cm, diameter 6.0cm.Then clean, with the tpn solution soaking disinfection 10min of 800 times, taking-up is dried, and for subsequent use as red cloud curcuma zedoary rhizome, rhizome diameter is 5.5 ~ 8.0cm.
refrigeration:red cloud curcuma zedoary rhizome is placed in freezer, at 10 DEG C, refrigerate 40 days.
vernalization:take out the red cloud curcuma zedoary rhizome of refrigeration, with clear water, after running water (water under room temperature) can be selected to soak 30 minutes, cover with the moistening fine sand of water content about 55%, at 28 DEG C of temperature, vernalization under dark condition, sprays 1 water after within 10 days, waiting for fine sand bone dry afterwards, will squirt completely from fine sand surface in the middle part of rhizome, then no longer moisture content is given, vernalization 30 days, treats that bud grows to 4 ~ 5cm, and taking-up is carried out water and supported.
water is supported:when water supports beginning, topped up with water in culture vessel, induces new root to grow bud point from rhizome, to maintain growth and the evaporation needs of 1 week; After cultivating 1 week, keep container completely anhydrous 2 ~ 3 days, during container overdrying, atomized water spray increases humidity; Add a small amount of moisture maintenance rhizome afterwards slowly to grow, continue about 3 ~ 4 days; Wait for that the bud mutation of red cloud curcuma zedoary becomes red, and rhizome dry tack free is to when omiting dehydration, recovers normal water and support management.
Water supports condition: water supports 20 days time, temperature 28 DEG C, intensity of illumination 2000LUX, relative air humidity (RH) 70%.Water is supported and is entered initial bloom stage after 20 days, and flowering rate reaches 85%, about 50 days inflorescence life-spans, and first spend the posterior lobe time interval to reach more than 20 days, the florescence is about the Spring Festival.
embodiment two: water is supported
gather and pretreatment:gather gather November then red cloud curcuma zedoary acrial part wither after red cloud curcuma zedoary rhizome, remove root tuber of aromatic turmeric and old root and fibrous root, retain complete lateral rhizome, lateral rhizome is about 8.0 ~ 10.0cm, diameter 6.0cm.Then clean, with the tpn solution soaking disinfection 10min of 800 times, taking-up is dried, and for subsequent use as red cloud curcuma zedoary rhizome, rhizome diameter is 5.5 ~ 8.0cm.
refrigeration:red cloud curcuma zedoary rhizome is placed in freezer, at 11 DEG C, refrigerate 60 days.
vernalization:take out the red cloud curcuma zedoary rhizome of refrigeration, with clear water, after running water (water under room temperature) can be selected to soak 20 minutes, cover with the moistening fine sand of water content about 55%, at 30 DEG C of temperature, vernalization under dark condition, sprays 1 water after within 10 days, waiting for fine sand bone dry afterwards, will squirt completely from fine sand surface in the middle part of rhizome, then no longer moisture content is given, vernalization 40 days, treats that bud grows to 4 ~ 5cm, and taking-up is carried out water and supported.
water is supported:when water supports beginning, topped up with water in culture vessel, induces new root to grow bud point from rhizome, to maintain growth and the evaporation needs of 1 week; After cultivating 1 week, keep container completely anhydrous 2.5 days, during container overdrying, atomized water spray increases humidity; Add a small amount of moisture maintenance rhizome afterwards slowly to grow, continue about 3.5 days; Wait for that the bud mutation of red cloud curcuma zedoary becomes red, and rhizome dry tack free is to when omiting dehydration, recovers normal water and support management.Water supports condition: water supports 15 days time, temperature 30 DEG C, intensity of illumination 3000LUX, relative air humidity (RH) 80%.Water is supported and is entered initial bloom stage after 15 days, flowering rate 65%, about 45 days inflorescence life-spans, first spends the posterior lobe time interval to reach more than 20 days.Florescence is about the Spring Festival.
embodiment three: water is supported
gather and pretreatment:gather gather November then red cloud curcuma zedoary acrial part wither after red cloud curcuma zedoary rhizome, remove root tuber of aromatic turmeric and old root and fibrous root, retain complete lateral rhizome, lateral rhizome is about 8.0 ~ 10.0cm, diameter 6.0cm.Then clean, with the tpn solution soaking disinfection 10min of 800 times, taking-up is dried, and for subsequent use as red cloud curcuma zedoary rhizome, rhizome diameter is 5.5 ~ 8.0cm.
refrigeration:red cloud curcuma zedoary rhizome is placed in freezer, at 13 DEG C, refrigerate 30 days.
vernalization:take out the red cloud curcuma zedoary rhizome of refrigeration, with clear water, after running water (water under room temperature) can be selected to soak 40 minutes, cover with the moistening fine sand of water content about 55%, at 28 DEG C of temperature, vernalization under dark condition, sprays 1 water after within 10 days, waiting for fine sand bone dry afterwards, will squirt completely from fine sand surface in the middle part of rhizome, then no longer moisture content is given, vernalization 35 days, treats that bud grows to 4 ~ 5cm, and taking-up is carried out water and supported.
water is supported:when water supports beginning, topped up with water in culture vessel, induces new root to grow bud point from rhizome, to maintain growth and the evaporation needs of 1 week; After cultivating 1 week, keep container completely anhydrous 2 days, during container overdrying, atomized water spray increases humidity; Add a small amount of moisture maintenance rhizome afterwards slowly to grow, continue about 3 days; Wait for that the bud mutation of red cloud curcuma zedoary becomes red, and rhizome dry tack free is to when omiting dehydration, recovers normal water and support management.Water supports condition: water supports 20 days time, temperature 30 DEG C, intensity of illumination 2500LUX, relative air humidity (RH) 75%.Water is supported and is entered initial bloom stage after 20 days, flowering rate 45%, about 50 days inflorescence life-spans, and first spend the posterior lobe time interval to reach more than 15 days, the florescence is about the Spring Festival.
embodiment four: water is supported
gather and pretreatment:gather gather November then red cloud curcuma zedoary acrial part wither after red cloud curcuma zedoary rhizome, remove root tuber of aromatic turmeric and old root, retain complete lateral rhizome, lateral rhizome is about 8.0 ~ 10.0cm, diameter 6.0cm.Then clean, with the tpn solution soaking disinfection 10min of 800 times, taking-up is dried, and for subsequent use as red cloud curcuma zedoary rhizome, rhizome diameter is 5.5 ~ 8.0cm.
refrigeration:red cloud curcuma zedoary rhizome is placed in freezer, at 13 DEG C, refrigerate 50 days.
vernalization:take out the red cloud curcuma zedoary rhizome of refrigeration, with clear water, after running water (water under room temperature) can be selected to soak 35 minutes, cover with the moistening fine sand of water content about 55%, at 28 DEG C of temperature, vernalization under dark condition, sprays 1 water after within 10 days, waiting for fine sand bone dry afterwards, will squirt completely from fine sand surface in the middle part of rhizome, then no longer moisture content is given, vernalization 30 days, treats that bud grows to 4 ~ 5cm, and taking-up is carried out water and supported.
water is supported:when water supports beginning, topped up with water in culture vessel, induces new root to grow bud point from rhizome, to maintain growth and the evaporation needs of 1 week; After cultivating 1 week, keep container completely anhydrous 3 days, during container overdrying, atomized water spray increases humidity; Add a small amount of moisture maintenance rhizome afterwards slowly to grow, continue about 4 days; Wait for that the bud mutation of red cloud curcuma zedoary becomes red, and rhizome dry tack free is to when omiting dehydration, recovers normal water and support management.Water supports condition: water supports 20 days time, temperature 28 DEG C, intensity of illumination 3000LUX, relative air humidity (RH) 80%.Water is supported and is entered initial bloom stage after 20 days, and flowering rate reaches 80%, about 50 days inflorescence life-spans, and first spend the posterior lobe time interval to reach more than 20 days, the florescence is about the Spring Festival.
In above embodiment, in order to protect rhizome can survive for a long time at low temperatures, and when vernalization for rhizome provides sufficient moisture, in refrigeration and vernalization, all clean culture medium can be covered on rhizome.Culture medium can be through the one in the sand of sterilization, peat soil and perlite, also can be the mixing of in them any two or three.Nutrient solution used during vernalization and water are supported is recommended to select MS nutrient solution, and it is a kind of low conductivity nutrient solution, containing macroelement and trace elements such as the nitrogen needed for plant, phosphorus, potassium, zinc, copper, molybdenum, iron.Because the Growth and anthesis of nutrient solution to red cloud curcuma zedoary of variable concentrations has a significant impact, concentration is too low, affects the normal absorption of red cloud curcuma zedoary, even may produce concentration difference and cause the dehydration of red cloud curcuma zedoary; Then can Developing restraint during high concentration, even make Plant death.Therefore, the concentration of MS nutrient solution is 1/4 ~ 1/32 times of MS nutrient solution mother liquid concentration, and preferred concentration is 1/4 ~ 1/16 times of MS nutrient solution mother liquid concentration.MS nutrient solution mother liquor is according to existing formulated.
The present invention can summarize with other the concrete form without prejudice to spirit of the present invention or principal character.Above-mentioned embodiment of the present invention all can only be thought explanation of the present invention instead of restriction, any change therefore in the implication suitable with claims of the present invention and scope, all should think to be included in the scope of claims.