CN101522899B - 安全性改善的表达载体 - Google Patents

安全性改善的表达载体 Download PDF

Info

Publication number
CN101522899B
CN101522899B CN2007800378795A CN200780037879A CN101522899B CN 101522899 B CN101522899 B CN 101522899B CN 2007800378795 A CN2007800378795 A CN 2007800378795A CN 200780037879 A CN200780037879 A CN 200780037879A CN 101522899 B CN101522899 B CN 101522899B
Authority
CN
China
Prior art keywords
promoter
cells
vector
seq
pgem
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN2007800378795A
Other languages
English (en)
Chinese (zh)
Other versions
CN101522899A (zh
Inventor
金善荣
金水晶
李准台
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Viromed Co ltd
Original Assignee
Viromed Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Viromed Co ltd filed Critical Viromed Co ltd
Publication of CN101522899A publication Critical patent/CN101522899A/zh
Application granted granted Critical
Publication of CN101522899B publication Critical patent/CN101522899B/zh
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
    • C12N2740/13041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/13043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
CN2007800378795A 2006-10-10 2007-10-10 安全性改善的表达载体 Expired - Fee Related CN101522899B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US60/850,269 2004-05-20
US85026906P 2006-10-10 2006-10-10
PCT/KR2007/004940 WO2008044869A1 (en) 2006-10-10 2007-10-10 Expression vectors with improved safety

Publications (2)

Publication Number Publication Date
CN101522899A CN101522899A (zh) 2009-09-02
CN101522899B true CN101522899B (zh) 2012-07-25

Family

ID=39283030

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2007800378795A Expired - Fee Related CN101522899B (zh) 2006-10-10 2007-10-10 安全性改善的表达载体

Country Status (6)

Country Link
US (1) US8105575B2 (https=)
EP (2) EP2261360B1 (https=)
JP (2) JP2010505442A (https=)
KR (1) KR101421063B1 (https=)
CN (1) CN101522899B (https=)
WO (1) WO2008044869A1 (https=)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010085660A2 (en) * 2009-01-23 2010-07-29 Roger Williams Hospital Viral vectors encoding multiple highly homologous non-viral polypeptides and the use of same
CN101864420B (zh) * 2009-05-06 2012-05-23 中国人民解放军军事医学科学院生物工程研究所 一种用于hek293细胞高效表达外源基因的表达载体
FR2986536A1 (fr) * 2012-02-08 2013-08-09 Lfb Biotechnologies Unites de transcription et leur utilisation dans des vecteurs d'expression (cho)
WO2014099671A1 (en) * 2012-12-20 2014-06-26 Bluebird Bio, Inc. Chimeric antigen receptors and immune cells targeting b cell malignancies
CN103074374B (zh) * 2013-01-16 2014-04-02 薛博夫 人β-NGF的重组表达载体及含该载体的重组细胞株
AR100419A1 (es) * 2014-05-14 2016-10-05 Esteve Labor Dr Vectores de virus adenoasociados para el tratamiento de trastornos de almacenamiento lisosómico
WO2016176152A2 (en) * 2015-04-25 2016-11-03 Washington State University Re-targeted foamy virus vectors
US11976302B2 (en) 2017-05-06 2024-05-07 Upside Foods, Inc. Compositions and methods for increasing the culture density of a cellular biomass within a cultivation infrastructure
US11479792B2 (en) 2017-07-13 2022-10-25 Upside Foods, Inc. Compositions and methods for increasing the efficiency of cell cultures used for food production
US11617788B2 (en) 2018-07-09 2023-04-04 Arizona Board Of Regents On Behalf Of The University Of Arizona Viral promoters and compositions and methods of use thereof
CA3153133A1 (en) * 2019-09-30 2021-04-08 Chris Bartolome Adeno-associated virus (aav) systems for treatment of genetic hearing loss
CN114901828A (zh) * 2019-12-30 2022-08-12 南京金斯瑞生物科技有限公司 一种哺乳动物表达系统使用的高表达载体
CN112250751B (zh) * 2020-10-22 2022-06-03 济南市中心医院 LENG8作为mRNA转录调控蛋白及线粒体活性调控蛋白的应用
WO2022133351A1 (en) * 2020-12-18 2022-06-23 Usmedigene Inc. Vectors for delivery of human growth hormone gene and multiple therapeutic genes into central nervous system by crossing blood brain barrier
WO2023240152A1 (en) 2022-06-07 2023-12-14 Upside Foods, Inc. Engineering cell lines capable of proliferation in growth factor free media formulations

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006078880A2 (en) * 2005-01-21 2006-07-27 Immusol Incorporated Inducible sirna expression cassette and method of use

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5583024A (en) 1985-12-02 1996-12-10 The Regents Of The University Of California Recombinant expression of Coleoptera luciferase
DE69123241T2 (de) 1990-12-14 1997-04-17 Cell Genesys Inc Chimärische ketten zur transduktion von rezeptorverbundenen signalwegen
AT401526B (de) 1993-02-10 1996-09-25 Scheirer Winfried Reagenzlösung zur stabilisierung der lumineszenz bei der luciferasemessung
US5976796A (en) 1996-10-04 1999-11-02 Loma Linda University Construction and expression of renilla luciferase and green fluorescent protein fusion genes
JP4390860B2 (ja) * 1997-05-13 2009-12-24 ユニヴァーシティ・オヴ・ノース・キャロライナ・アト・チャペル・ヒル レンチウイルスをベースにした遺伝子転移ベクター
US6074859A (en) 1997-07-08 2000-06-13 Kikkoman Corporation Mutant-type bioluminescent protein, and process for producing the mutant-type bioluminescent protein
KR20000006334A (ko) * 1998-06-26 2000-01-25 이선경 바이러스코딩염기서열이전혀없는고효율레트로바이러스벡터
KR20000046969A (ko) 1998-12-31 2000-07-25 이선경 이종 유전자 유래의 유전자 발현 조절요소를 포함하는 강력한전사 활성을 가진 진핵세포 발현벡더
KR100373821B1 (ko) * 2000-09-08 2003-02-26 주식회사 바이로메드 세포에서 유래한 변형된 비코딩 서열을 갖는 개선된고효율 레트로바이러스 벡터
US7575924B2 (en) * 2000-11-13 2009-08-18 Research Development Foundation Methods and compositions relating to improved lentiviral vectors and their applications
AU2006214278C1 (en) 2005-02-16 2012-07-19 Miltenyi Biotec Technology, Inc. Lentiviral vectors and their use
GB0509965D0 (en) * 2005-05-17 2005-06-22 Ml Lab Plc Improved expression elements
US20070161031A1 (en) * 2005-12-16 2007-07-12 The Board Of Trustees Of The Leland Stanford Junior University Functional arrays for high throughput characterization of gene expression regulatory elements

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006078880A2 (en) * 2005-01-21 2006-07-27 Immusol Incorporated Inducible sirna expression cassette and method of use

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Sara J. Cooper等.Comprehensive analysis……human genome.《Genome Research》.2006,第16卷(第1期),1-10. *

Also Published As

Publication number Publication date
JP2013143947A (ja) 2013-07-25
WO2008044869A1 (en) 2008-04-17
CN101522899A (zh) 2009-09-02
JP5760023B2 (ja) 2015-08-05
KR101421063B1 (ko) 2014-07-21
KR20090092755A (ko) 2009-09-01
EP2082043A4 (en) 2010-03-24
EP2261360B1 (en) 2014-04-23
JP2010505442A (ja) 2010-02-25
EP2082043A1 (en) 2009-07-29
US8105575B2 (en) 2012-01-31
EP2261360A1 (en) 2010-12-15
US20080153770A1 (en) 2008-06-26

Similar Documents

Publication Publication Date Title
CN101522899B (zh) 安全性改善的表达载体
US7226780B2 (en) Lentivirus vector system
US20050124564A1 (en) Anemia
CA2328404C (en) Novel lentiviral packaging cells
JP4612950B2 (ja) レトロウイルス送達システム
KR20160122125A (ko) 바이러스 벡터 생산 시스템
JP7759318B2 (ja) 産生系
NZ503318A (en) Modified haemopoietic stem cell comprised of a spliced upstream with a nucleotide sequence of interest to be used as in gene therapy
US6818209B1 (en) Retroviral delivery system
US20230174600A1 (en) Compositions and methods for the treatment of cystic fibrosis
WO2000040741A2 (en) Lentivirus vector system
JPWO2006090689A1 (ja) Ｓｉｖ−ｐｅｄｆベクターを用いた眼組織細胞におけるアポトーシス変性を伴う疾患の治療薬
WO2001083730A2 (en) Recombinant lentiviral vectors pseudotyped in envelopes containing filovirus binding domains
EP1548101A1 (en) Methods of producing a viral vector comprising a membrane protein that binds to sialic acid as a component of the envelope using neuraminidase derived from gram-positive bacteria
US20040033604A1 (en) Recombinant lentiviral vectors pseudotyped in envelopes containing filovirus binding domains
WO2025199323A1 (en) Methods and compositions for viral vector production via partial producer libraries
HK1077083A (en) Methods of producing a viral vector comprising a membrane protein that binds to sialic acid as a component of the envelope using neuraminidase derived from gram-positive bacteria
HK1056576B (en) Pseudotype retroviral vectors containing membrane proteins having hemagglutinin activity
HK1056576A1 (zh) 含有血凝素活性膜蛋白质的假型逆转录病毒载体

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20120725

CF01 Termination of patent right due to non-payment of annual fee