CN101522899B - 安全性改善的表达载体 - Google Patents

安全性改善的表达载体 Download PDF

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Publication number
CN101522899B
CN101522899B CN2007800378795A CN200780037879A CN101522899B CN 101522899 B CN101522899 B CN 101522899B CN 2007800378795 A CN2007800378795 A CN 2007800378795A CN 200780037879 A CN200780037879 A CN 200780037879A CN 101522899 B CN101522899 B CN 101522899B
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sequence
cell
promotor
retrovirus vector
seq
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CN2007800378795A
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English (en)
Chinese (zh)
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CN101522899A (zh
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金善荣
金水晶
李准台
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Viromed Co ltd
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Viromed Co ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
    • C12N2740/13041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/13043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
CN2007800378795A 2006-10-10 2007-10-10 安全性改善的表达载体 Expired - Fee Related CN101522899B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US85026906P 2006-10-10 2006-10-10
US60/850,269 2006-10-10
PCT/KR2007/004940 WO2008044869A1 (en) 2006-10-10 2007-10-10 Expression vectors with improved safety

Publications (2)

Publication Number Publication Date
CN101522899A CN101522899A (zh) 2009-09-02
CN101522899B true CN101522899B (zh) 2012-07-25

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CN2007800378795A Expired - Fee Related CN101522899B (zh) 2006-10-10 2007-10-10 安全性改善的表达载体

Country Status (6)

Country Link
US (1) US8105575B2 (enExample)
EP (2) EP2261360B1 (enExample)
JP (2) JP2010505442A (enExample)
KR (1) KR101421063B1 (enExample)
CN (1) CN101522899B (enExample)
WO (1) WO2008044869A1 (enExample)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9206440B2 (en) * 2009-01-23 2015-12-08 Roger Williams Hospital Viral vectors encoding multiple highly homologus non-viral polypeptides and the use of same
CN101864420B (zh) * 2009-05-06 2012-05-23 中国人民解放军军事医学科学院生物工程研究所 一种用于hek293细胞高效表达外源基因的表达载体
FR2986536A1 (fr) 2012-02-08 2013-08-09 Lfb Biotechnologies Unites de transcription et leur utilisation dans des vecteurs d'expression (cho)
WO2014099671A1 (en) * 2012-12-20 2014-06-26 Bluebird Bio, Inc. Chimeric antigen receptors and immune cells targeting b cell malignancies
CN103074374B (zh) * 2013-01-16 2014-04-02 薛博夫 人β-NGF的重组表达载体及含该载体的重组细胞株
MX383227B (es) * 2014-05-14 2025-03-13 UNIV AUTòNOMA DE BARCELONA Vectores de virus adenoasociados para el tratamiento de trastornos de almacenamiento lisosomico.
WO2016176152A2 (en) * 2015-04-25 2016-11-03 Washington State University Re-targeted foamy virus vectors
ES2985769T3 (es) 2017-05-06 2024-11-07 Upside Foods Inc Composiciones y métodos para aumentar la densidad de cultivo de una biomasa celular dentro de una infraestructura de cultivo
WO2019014652A1 (en) 2017-07-13 2019-01-17 Memphis Meats, Inc. COMPOSITIONS AND METHODS FOR INCREASING THE EFFICACY OF CELL CULTURES USED IN FOOD PRODUCTION
WO2020014222A2 (en) * 2018-07-09 2020-01-16 Arizona Board Of Regents On Behalf Of The University Of Arizona Viral promoters and compositions and methods of use thereof
AU2020357740A1 (en) * 2019-09-30 2022-04-28 Eli Lilly And Company Adeno-associated virus (AAV) systems for treatment of genetic hearing loss
WO2021136347A1 (zh) * 2019-12-30 2021-07-08 南京金斯瑞生物科技有限公司 一种哺乳动物表达系统使用的高表达载体
CN112250751B (zh) * 2020-10-22 2022-06-03 济南市中心医院 LENG8作为mRNA转录调控蛋白及线粒体活性调控蛋白的应用
US20250281641A1 (en) * 2020-12-18 2025-09-11 Usmedigene Inc. Vectors for delivery of human growth hormone gene and multiple therapeutic genes into central nervous system by crossing blood brain barrier

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006078880A2 (en) * 2005-01-21 2006-07-27 Immusol Incorporated Inducible sirna expression cassette and method of use

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US5583024A (en) 1985-12-02 1996-12-10 The Regents Of The University Of California Recombinant expression of Coleoptera luciferase
AU643109B2 (en) 1990-12-14 1993-11-04 Cell Genesys, Inc. Chimeric chains for receptor-associated signal transduction pathways
AT401526B (de) 1993-02-10 1996-09-25 Scheirer Winfried Reagenzlösung zur stabilisierung der lumineszenz bei der luciferasemessung
US5976796A (en) 1996-10-04 1999-11-02 Loma Linda University Construction and expression of renilla luciferase and green fluorescent protein fusion genes
KR100556864B1 (ko) * 1997-05-13 2006-03-10 더 유니버시티 오브 노쓰 캐롤라이나 엣 채플 힐 렌티바이러스-기원 유전자 전달 벡터
US6074859A (en) 1997-07-08 2000-06-13 Kikkoman Corporation Mutant-type bioluminescent protein, and process for producing the mutant-type bioluminescent protein
KR20000006334A (ko) * 1998-06-26 2000-01-25 이선경 바이러스코딩염기서열이전혀없는고효율레트로바이러스벡터
KR20000046969A (ko) 1998-12-31 2000-07-25 이선경 이종 유전자 유래의 유전자 발현 조절요소를 포함하는 강력한전사 활성을 가진 진핵세포 발현벡더
KR100373821B1 (ko) * 2000-09-08 2003-02-26 주식회사 바이로메드 세포에서 유래한 변형된 비코딩 서열을 갖는 개선된고효율 레트로바이러스 벡터
US7575924B2 (en) * 2000-11-13 2009-08-18 Research Development Foundation Methods and compositions relating to improved lentiviral vectors and their applications
EP1858332A4 (en) 2005-02-16 2011-06-22 Lentigen Corp LENTIVIRUS VECTORS AND ITS USE
GB0509965D0 (en) * 2005-05-17 2005-06-22 Ml Lab Plc Improved expression elements
US20070161031A1 (en) * 2005-12-16 2007-07-12 The Board Of Trustees Of The Leland Stanford Junior University Functional arrays for high throughput characterization of gene expression regulatory elements

Patent Citations (1)

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Publication number Priority date Publication date Assignee Title
WO2006078880A2 (en) * 2005-01-21 2006-07-27 Immusol Incorporated Inducible sirna expression cassette and method of use

Non-Patent Citations (1)

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Title
Sara J. Cooper等.Comprehensive analysis……human genome.《Genome Research》.2006,第16卷(第1期),1-10. *

Also Published As

Publication number Publication date
EP2082043A1 (en) 2009-07-29
KR101421063B1 (ko) 2014-07-21
WO2008044869A1 (en) 2008-04-17
JP2010505442A (ja) 2010-02-25
EP2261360B1 (en) 2014-04-23
US8105575B2 (en) 2012-01-31
JP5760023B2 (ja) 2015-08-05
EP2261360A1 (en) 2010-12-15
EP2082043A4 (en) 2010-03-24
KR20090092755A (ko) 2009-09-01
JP2013143947A (ja) 2013-07-25
CN101522899A (zh) 2009-09-02
US20080153770A1 (en) 2008-06-26

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