CN101513163B - Method for increasing the contents of steroid saponin in paris polyphylla rhizome by penicillium citrinum elicitor - Google Patents
Method for increasing the contents of steroid saponin in paris polyphylla rhizome by penicillium citrinum elicitor Download PDFInfo
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- CN101513163B CN101513163B CN2009100614214A CN200910061421A CN101513163B CN 101513163 B CN101513163 B CN 101513163B CN 2009100614214 A CN2009100614214 A CN 2009100614214A CN 200910061421 A CN200910061421 A CN 200910061421A CN 101513163 B CN101513163 B CN 101513163B
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- elicitor
- penicillium citrinum
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- paris polyphylla
- steroid saponin
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Abstract
The invention discloses a method for increasing the contents of steroid saponin in paris polyphylla rhizome by penicillium citrinum elicitor, which comprises the following steps: penicillium citrinum elicitor is dissolved in water and prepared into a penicillium citrinum elicitor solution with the concentration of 76-304mgGE/L, the leaves of the paris polyphylla which expand completely are uniformly sprayed with the penicillium citrinum elicitor solution in the July and October of a calendar year till when the penicillium citrinum elicitor solution on the leaves drops, the spraying is stopped and sprayed every year. The above operation is carried out every year till the paris polyphylla rhizome can be used as medicine. The needed medicine and apparatus are simple and cheap, has less investment and low cost; therefore, the method is easy to be popularized. The implementation process is easy, can obviously increase the contents of the steroid saponin in paris polyphylla rhizome and causes the total contents of steroid saponin in the current year-grew rhizome and the former-year rhizome to respectively increase by 54% and 97%. And the method does not poison people and animals and has no pollution to the environment.
Description
Technical field
The present invention relates to a kind of method that improves contents of steroid saponin in the paris polyphylla rhizome, particularly relate to a kind of method of utilizing penicillium citrinum elicitor to promote contents of steroid saponin in the paris polyphylla rhizome.
Background technology
Yunnan Rhizoma Paridis is the herbaceos perennial of Trilliaceae Paris, mainly is distributed in ground such as southwest China, particularly Yunnan, Sichuan and Guizhou.Yunnan Rhizoma Paridis is famous and precious medicinal plant, and the steroid saponin compound that contains in its root-like stock mainly comprises pennogenin and Dioscin, is its main active, has anticancer, hemostasis, anti-inflammatory, analgesia, relieving cough and asthma and immunological regulation isoreactivity.The Yunnan Rhizoma Paridis growth cycle is long, reproduction rate is low, biomass accumulation is few.In recent years, along with market increasing rapidly to its demand, people are in the open air to its excavating wantonly, make Yunnan Rhizoma Paridis resource distribution fragmentation, its growth and procreation have been had a strong impact in the original producton location, cause Yunnan Rhizoma Paridis progressively to step into the endangered plants ranks, the industrial medicinal raw material of Paris polyphylla occurs seriously in short supply.Therefore it is extremely urgent it to be carried out artificial domesticating cultivation.
Utilize the secondary metabolism approach in the pathogen elicitor activated plant cell to become one of effective means that improves target product output in the plant cell cultures.Penicillium citrinum elicitor is a kind of fungal elicitor, can be fast in the interaction of plant and fungi, the expression of inducing plant specific gene selectively, and then activate specific secondary metabolism approach, accumulate specific purpose secondary metabolite, be widely used in promoting the synthetic of secondary metabolite in the various plants cell.
Do not occur utilizing penicillium citrinum elicitor to promote rhizomatic growth in the Yunnan Rhizoma Paridis at present as yet, improve the wherein method of contents of steroid saponin.
Summary of the invention
The objective of the invention is to remedy the deficiencies in the prior art, a kind of method that improves contents of steroid saponin in the paris polyphylla rhizome with penicillium citrinum elicitor is provided.
For achieving the above object, the technical solution used in the present invention is: a kind of method with contents of steroid saponin in the penicillium citrinum elicitor raising paris polyphylla rhizome may further comprise the steps:
(1) penicillium citrinum elicitor is water-soluble, being mixed with concentration is the penicillium citrinum elicitor solution of 76～304mgGE/L;
(2) 7～August of a calendar year to the exhibition leaf completely the Yunnan Rhizoma Paridis blade evenly spray penicillium citrinum elicitor solution one time, the penicillium citrinum elicitor solution on blade stops to spray in the time of will dripping.
Above-mentioned steps is that penicillium citrinum elicitor is water-soluble in (1), and being mixed with concentration is the penicillium citrinum elicitor solution of 304mgGE/L.
Be in the above-mentioned steps (2) the dusk time-division, to the exhibition leaf completely the Yunnan Rhizoma Paridis blade evenly spray penicillium citrinum elicitor solution one time.
In the above-mentioned steps (2) be to the exhibition leaf completely the tow sides of Yunnan Rhizoma Paridis blade evenly spray penicillium citrinum elicitor solution one time.
In the above-mentioned steps (2) be 7～August in every year to the exhibition leaf completely the Yunnan Rhizoma Paridis blade evenly spray penicillium citrinum elicitor solution one time.
Experimental result shows, adopting concentration among the present invention is the penicillium citrinum elicitor solution of 304mgGE/L, in every year the 7～August in the dusk time-division, to the exhibition leaf completely the blade tow sides of Yunnan Rhizoma Paridis plant evenly spray once, can be used as medicine until its root-like stock, can reach optimum efficiency.
Advantage of the present invention is as follows:
(1) required medicine, instrument and equipment simple cheap only need the preparation penicillium citrinum elicitor, and add the equipment of spraying (as watering can or sprayer) and can implement, so less investment, cost is low;
(2) implementation process is simple, make Yunnan Rhizoma Paridis give birth to root-like stock, former years then and give birth to that total steroid saponin content has improved 54%, 97% respectively in the root-like stock, and this method does not have influence to people and animals' nonhazardous to environment.
Embodiment
The invention will be further described below by specific embodiment.Penicillium citrinum elicitor used in the embodiment of the invention utilizes prior art for preparing.
Embodiment 1
Penicillium citrinum elicitor is water-soluble, being made into concentration is the penicillium citrinum elicitor solution of 76mgGE/L, in in every year the 7～August in the dusk time-division, to the exhibition leaf completely the blade tow sides of Yunnan Rhizoma Paridis plant evenly spray once, the penicillium citrinum elicitor solution on blade stops to spray in the time of will dripping.Other gets the water with the above-mentioned amount of spraying equivalent, adopt with quadrat method to the exhibition leaf completely the blade tow sides on the Yunnan Rhizoma Paridis plant evenly spray in contrast.In case of rainy, can fill spray.Treat that Yunnan Rhizoma Paridis falls behind the seedling, detects contents of steroid saponin in the root-like stock with the RP-HPLC method.The result shows that total steroid saponin content has improved 24%, 53% respectively in living then root-like stock, life in the former years root-like stock.
Embodiment 2
Adopt with embodiment 1 similar methods to experimentize, it is the blade spraying of the penicillium citrinum elicitor solution of 152mgGE/L to Yunnan Rhizoma Paridis that difference is to adopt concentration.The result shows that total steroid saponin content has improved 15%, 42% respectively in living then root-like stock, life in the former years root-like stock.
Embodiment 3
Adopt with embodiment 1 similar methods to experimentize, it is the blade spraying of the penicillium citrinum elicitor solution of 228mgGE/L to Yunnan Rhizoma Paridis that difference is to adopt concentration.The result shows that total steroid saponin content has improved 18%, 89% respectively in living then root-like stock, life in the former years root-like stock.
Embodiment 4
Adopt with embodiment 1 similar methods to experimentize, it is the blade spraying of the penicillium citrinum elicitor solution of 304mgGE/L to Yunnan Rhizoma Paridis that difference is to adopt concentration.The result shows that total steroid saponin content has improved 54%, 97% respectively in living then root-like stock, life in the former years root-like stock.
Claims (5)
1. one kind is improved the method for contents of steroid saponin in the paris polyphylla rhizome with penicillium citrinum elicitor, it is characterized in that may further comprise the steps:
(1) penicillium citrinum elicitor is water-soluble, being mixed with concentration is the penicillium citrinum elicitor solution of 76～304mgGE/L;
(2) in 7～August of a calendar year, to the exhibition leaf completely the Yunnan Rhizoma Paridis blade evenly spray penicillium citrinum elicitor solution one time, the penicillium citrinum elicitor solution on blade stops to spray in the time of will dripping.
2. the method with contents of steroid saponin in the penicillium citrinum elicitor raising paris polyphylla rhizome according to claim 1, it is characterized in that: step is that penicillium citrinum elicitor is water-soluble in (1), being mixed with concentration is the penicillium citrinum elicitor solution of 304mgGE/L.
3. according to claim 1ly improve the method for contents of steroid saponin in the paris polyphylla rhizome, it is characterized in that with penicillium citrinum elicitor: be in the step (2) the dusk time-division, to the exhibition leaf completely the Yunnan Rhizoma Paridis blade evenly spray penicillium citrinum elicitor solution one time.
4. according to each describedly improves the method for contents of steroid saponin in the paris polyphylla rhizome with penicillium citrinum elicitor in the claim 1～3, it is characterized in that: in the step (2) be to the exhibition leaf completely the tow sides of Yunnan Rhizoma Paridis blade evenly spray penicillium citrinum elicitor solution one time.
5. according to each described method that improves contents of steroid saponin in the paris polyphylla rhizome with penicillium citrinum elicitor in the claim 1～3, it is characterized in that: be 7～August in the step (2) in every year, to the exhibition leaf completely the Yunnan Rhizoma Paridis blade evenly spray penicillium citrinum elicitor solution one time, can be used as medicine until its root-like stock.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100614214A CN101513163B (en) | 2009-04-03 | 2009-04-03 | Method for increasing the contents of steroid saponin in paris polyphylla rhizome by penicillium citrinum elicitor |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2009100614214A CN101513163B (en) | 2009-04-03 | 2009-04-03 | Method for increasing the contents of steroid saponin in paris polyphylla rhizome by penicillium citrinum elicitor |
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| CN101513163A CN101513163A (en) | 2009-08-26 |
| CN101513163B true CN101513163B (en) | 2011-05-18 |
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| CN2009100614214A Expired - Fee Related CN101513163B (en) | 2009-04-03 | 2009-04-03 | Method for increasing the contents of steroid saponin in paris polyphylla rhizome by penicillium citrinum elicitor |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1210663A (en) * | 1998-09-08 | 1999-03-17 | 中国科学院昆明植物研究所 | Seed sprouting and quick propagating technology of Yunnan Rhizoma Paridis |
| CN1490320A (en) * | 2003-08-11 | 2004-04-21 | 云南白药集团天然药物研究院 | Preparation of paris polyphylla saponin |
| CN1562064A (en) * | 2004-03-22 | 2005-01-12 | 深圳中药及天然药物研究中心 | New use of saponin compound for treating cardiovascular disease |
| CN101213914A (en) * | 2008-01-09 | 2008-07-09 | 丽江华利生物开发有限责任公司 | Yunnan rhizoma paridis rhizocaul multiple germination raise-seedling method |
-
2009
- 2009-04-03 CN CN2009100614214A patent/CN101513163B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1210663A (en) * | 1998-09-08 | 1999-03-17 | 中国科学院昆明植物研究所 | Seed sprouting and quick propagating technology of Yunnan Rhizoma Paridis |
| CN1490320A (en) * | 2003-08-11 | 2004-04-21 | 云南白药集团天然药物研究院 | Preparation of paris polyphylla saponin |
| CN1562064A (en) * | 2004-03-22 | 2005-01-12 | 深圳中药及天然药物研究中心 | New use of saponin compound for treating cardiovascular disease |
| CN101213914A (en) * | 2008-01-09 | 2008-07-09 | 丽江华利生物开发有限责任公司 | Yunnan rhizoma paridis rhizocaul multiple germination raise-seedling method |
Non-Patent Citations (1)
| Title |
|---|
| 杨斌等.滇重楼的栽培与合理利用.《中国野生植物资源》.2008,第27卷(第6期),第70-73页. * |
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