CN101519325A - Foliage promoter for improving camptothecine content of camptotheca acuminata - Google Patents
Foliage promoter for improving camptothecine content of camptotheca acuminata Download PDFInfo
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- CN101519325A CN101519325A CN200910038548A CN200910038548A CN101519325A CN 101519325 A CN101519325 A CN 101519325A CN 200910038548 A CN200910038548 A CN 200910038548A CN 200910038548 A CN200910038548 A CN 200910038548A CN 101519325 A CN101519325 A CN 101519325A
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- camptothecine
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- camptotheca acuminata
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Abstract
The invention discloses a foliage promoter for improving the camptothecine content of camptotheca acuminata, which relates to a vegetable fertilizer, in particular to a foliage fertilizer for improving the camptothecine content of the camptotheca acuminata. The foliage promoter is prepared from the following raw materials by weight portion: 10 to 12 portions of heavy metallic salt, 5 to 10 portions of rare earth, 1 to 5 portions of organic acid and 1 to 5 portions of vegetable growth regulator. The foliage promoter can dramatically improve the camptothecine content of camptotheca acuminata, can be conveniently applied by directly spraying, adopts simply-operated indoor shading for water retention and is easy to popularize; the effect of the foliage promoter is quick as the camptothecine content of camptotheca acuminata can be improved dramatically after spraying the foliage for twice; the efficiency of the foliage promoter is high, as the foliage promoter can promote the full utilization of the camptotheca acuminate resource and protect the camptotheca acuminata resource and ecological environment; and the economic efficiency of the foliage promoter is remarkable.
Description
Technical field
The present invention relates to be used for the fertilizer of plant, specifically is the foliage fertilizer that is used for improving the camplotheca acuminata camptothecine content.
Background technology
Camplotheca acuminata (Camptotheca acuminata Decne) is that Nyssaceae (Nyssaceae) camplotheca acuminata belongs to (Camptotheca Decne.) plant, is distributed widely in the Asia, and it is especially many to distribute in China, is China's south endemic tree.Classify first national key protected wild plants (II level) through State Council approved in August, 1999.Camplotheca acuminata is the main natural origin of monoterpene indole alkaloid camptothecine.The present annual camptothecine demand in world market 3000kg, and available stock 1000kg only.
Camptothecine has antitumour activity as the inhibitor of a kind of topoisomerase (Topoisor-merase), proves through clinical trial, and camptothecine all has obvious curative effects to kind of tumour surplus 30, and toxic side effect is little, has high clinical value.And by camptothecine be raw material can prepare series derivative, as hydroxycamptothecine, topotecan, irinotecan, 9-AC (9-aminocamptothecin) and 9-NC (9-nitrocamptothecin) etc., all be that " topoisomerase enzyme inhibitor " is the extremely promising PTS of a class.
The content of camptothecine is very little in camplotheca acuminata, with the higher tender leaf of relative content is example, (L ó pez-Meyer M such as L ó pez-Meyer, Nessler CL, McKnight TD (1994) Sites of accumulationof the antitumor alkaloid camptothecin in Camptotheca acuminata.Plantamedica 60:558-560.), (Liu Z such as Liu, Carpenter SB, Bourgeois WJ, etal. (1998) Variations in the secondary metabol itecamptothecin inrelation to tissue age and season in Camptotheca acuminata.Tree Physiol18:265-270.) each position camptothecine content in the Camptotheca acuminata seedlings is analyzed, and find that the content in the camplotheca acuminata tender leaf is the highest, be about between 0.017% to 0.4%; But numerous documents show that the content of camptothecine also only is about 0.1% (Zhang Yuhong, Zu YuanGangs in the tender leaf of various places self-sow camplotheca acuminata.Camptothecine content is measured in the different places of production and the season of growth leaf of Common Camptotheca.BULLETIN OF BOTANY Vol..2003，20(5)：572-575)。For satisfying present and following potential pharmaceutical drugs demand, improve in the camplotheca acuminata camptothecine content, become the focus of countries in the world scientist's concern.
For this reason, the inventor to how to improve in the camplotheca acuminata camptothecine content made number of research projects.
Summary of the invention
The objective of the invention is to improve in the camplotheca acuminata camptothecine content.
To achieve the object of the present invention, the technical scheme that is adopted is: the foliage promoter of a kind of camplotheca acuminata is provided, and it is the pulvis of being made by the raw material of following weight part:
10～12 parts of heavy metallic salts
5～10 parts of rare earths
1～5 part of organic acid
1～5 part of plant-growth regulator
Heavy metallic salt has a lot, as copper sulfate, zinc sulfate, Silver Nitrate, mercury chloride etc. all can, as long as select wherein a kind of or two kinds, preferably sulfuric acid copper of the present invention or zinc sulfate.
Said organic acid can be that propanedioic acid, oxysuccinic acid, toxilic acid, citric acid, xitix etc. are all passable, as long as select wherein a kind of or two kinds, preferred propanedioic acid of the present invention or oxysuccinic acid.
Said plant-growth regulator has choline dichloride (chlormequat), choline chloride 60, paclobutrazol (paclobutrazal), alar-85 (Daminozide, have another name called daminozide), ethrel, acetylsalicylic acid, methyl jasmonate, triacontanol price quote, thiocarbamide, sodium bisulfite etc. all can, as long as select wherein a kind of, preferred choline dichloride of the present invention or choline chloride 60 or paclobutrazol or alar-85.
A lot of on the said rare-earth industry, the product of different manufacturers also has difference, if any rare earth chloride, and mixed rare earth carbonate, cerous nitrate, cerous acetate all can, (technical grade contains Ce:27% to the preferred rare earth chloride of the present invention, La:17%, Ca:1.5%, Mg:1.5%, Ba:0.3%; Fe:0.007%).
Product provided by the present invention is different for different camplotheca acuminata using method.Be used for beginning result's adult camplotheca acuminata, 2 weeks before gather camplotheca acuminata branches and leaves or fruit are with foliage promoter of the present invention branches and leaves and the fruit of evenly spraying soluble in water; Then before branches and leaves or fruit harvesting, again repeat spraying once, and gather branches and leaves or fruit at once.The indoor concealment in back of gathering is preserved moisture and was placed 3-5 days, and the nature of not preserving moisture then dries in the shade, and extracts camptothecine.Spray dose squirting each face of branches and leaves and fruit, but drops physic liquor is not degree.
For the camplotheca acuminata treelet of coming to nothing, can gather preceding 1 week at branches and leaves, with the foliage promoter of the present invention branches and leaves that evenly spray soluble in water; Then before branches and leaves are gathered, again repeat spraying once, and the branches and leaves of gathering at once.The indoor concealment in back of gathering is preserved moisture and was placed 3-5 days, and the nature of not preserving moisture then dries in the shade, and extracts camptothecine.Spray dose squirting each face of branches and leaves, but drops physic liquor is not degree.
For short close cultivation children camplotheca acuminata in age,, evenly spray branches and leaves with foliage promoter of the present invention when Camptotheca acuminata seedlings begins to seal when capable; One week back before branches and leaves are gathered, again repeat spraying once, and the branches and leaves of gathering at once.The indoor concealment in back of gathering is preserved moisture and was placed 3-5 days, and the nature of not preserving moisture then dries in the shade, and extracts camptothecine.Spray dose squirting each face of branches and leaves, but drops physic liquor is not degree.
The invention has the beneficial effects as follows:
1. used foliage promoter of the present invention can significantly improve the content of the total camptothecine of camplotheca acuminata.
2. this foliage promoter can directly spray, and uses conveniently, adopts the indoor concealment in back and preserves moisture simple to operately, is easy to promote.
3. this foliage promoter instant effect, only need the gather the last week and the eve of gathering spray twice and just can increase substantially camptothecine content in the camplotheca acuminata.Thereby increase substantially the output of camptothecine, the efficient height can promote making full use of of camplotheca acuminata resource, protection camplotheca acuminata resource and ecotope.
4. use this foliage promoter remarkable in economical benefits.Close (camplotheca acuminata of the cultivation of 80cm * 80cm) is an example with short, with about 10 yuan of the aqueous solution (mu consumption) raw materials cost of foliage promoter provided by the present invention configuration 100kg, spray about 20 yuan for twice, spray for twice and indoor about 50 yuan of the artificial input that work increased such as preserve moisture, with low cost; The about 100kg of dried branches and leaves that gathers, it is about 0.1% not spray the camptothecine content of foliage promoter, and extraction yield is about 80%, can get camptothecine 80g; Similarity condition if increase by 30% calculating by camptothecine content after spraying foliage promoter, can get camptothecine 104g, calculates by 60000 yuan/kg of present camptothecine, can increase income 1440 yuan, and income is 20 times of input cost.Therefore, economic benefit of the present invention is very remarkable.
Further set forth technical solution of the present invention and beneficial effect below in conjunction with embodiment.But be not limited to following embodiment.
Embodiment
The preparation of embodiment 1 foliage promoter
Take by weighing copper sulfate 10g (agricultural grade, content is more than or equal to 98%)
Propanedioic acid 1g (technical grade, content is more than or equal to 99.5%)
(technical grade contains Ce:27% to rare earth chloride 5g, La:17%, Ca:1.5%, Mg:1.5%, Ba:0.3%; Fe:0.007%)
Choline dichloride 1g (the former medicine of agricultural grade is more than or equal to 98%)
Alleged raw material is pulverized mixing promptly with conventional method.
The foliage promoter of gained can be dissolved in during use 20 kg of water can in spray.
The preparation of embodiment 2 foliage promoters
Take by weighing copper sulfate 12g (agricultural grade, content is more than or equal to 98%)
Propanedioic acid 2g (technical grade, content is more than or equal to 99.5%)
(technical grade contains Ce:27% to rare earth chloride 2g, La:17%, Ca:1.5%, Mg:1.5%, Ba:0.3%; Fe:0.007%)
Choline dichloride 2g (the former medicine of agricultural grade is more than or equal to 98%)
Alleged raw material is pulverized mixing promptly with conventional method.
The foliage promoter of gained can be dissolved in 20 kg of water during use and spray.
The preparation of embodiment 3 foliage promoters
Take by weighing copper sulfate 11g (agricultural grade, content is more than or equal to 98%)
Propanedioic acid 5g (technical grade, content is more than or equal to 99.5%)
(technical grade contains Ce:27% to rare earth chloride 5g, La:17%, Ca:1.5%, Mg:1.5%, Ba:0.3%; Fe:0.007%)
Choline dichloride 5g (the former medicine of agricultural grade is more than or equal to 98%)
Alleged raw material is pulverized mixing promptly with conventional method.
The foliage promoter of gained can be dissolved in 20 kg of water during use and spray.
When raw material is used zinc sulfate (agricultural grade instead, content is more than or equal to 98%), oxysuccinic acid (technical grade, content is more than or equal to 99.5%), choline chloride 60 (the former medicine of feed grade, more than or equal to 98%), paclobutrazol (the former medicine of agricultural grade, more than or equal to 90%), during alar-85 (the former medicine of agricultural grade is more than or equal to 92%) etc. also with above proportioning and use.
The adult camplotheca acuminata that embodiment 4 is used for beginning the result experimentizes
Camplotheca acuminata grows up to set greatly and plants in Guangzhou Tianhe District dragon's cave-stalactite cave Guangdong Institute of Traditional Chinese Medicine Chinese herbal medicine specimen garden, transplants in 1985, and the diameter of a cross-section of a tree trunk 1.3 meters above the ground reaches 30 centimetres, and highly about 12 meters, about 8 meters of crown diameter, it is good to grow, and branches and leaves are luxuriant.
On October 24th, 2007, in the week before picking fruit, the big tree of selected 6 identical growing way unanimities of the age of tree is divided into two groups (treatment group and control groups) at random.Treatment group sprays the foliage promoter of embodiment 1, sprays dose squirting each face of branches and leaves and fruit, but drops physic liquor is not degree.The foliage promoter that sprays embodiment 1 once more at picking fruit eve November 1 sprays dose squirting each face of branches and leaves and fruit then, but drops physic liquor is not degree.Control group sprays clear water twice the same period.On November 1st, 2007, picking fruit and collect the indoor concealment in back of gathering of 1 kilogram in branches and leaves and preserve moisture and placed 3 days, the nature of not preserving moisture then dries in the shade, and pulverizes.Extract and measuring method with reference to Yan Xiufeng, etc. reported method, document " Yan Xiufeng, Wang Yang,, Zhang Yuhong, Yin Lijun, the efficient liquid phase chromatographic analysis analytical test journal of camptothecine content in the leaf of Common Camptotheca, 2002,21 (2): 15-18 " in great waves
Concrete detection method is as follows.
The extraction of camptothecine: accurately take by weighing 0.4 gram drying material, pour in 50 milliliters of Erlenmeyer flasks, add 25 milliliter 75% ethanol.After weighing, place ultrasonic apparatus, 50 ℃ were extracted 10 minutes.After being chilled to room temperature, refill the amount of liquid evaporation with 75% ethanol.0.45 micron filtering membrane of this extracting solution and 10 μ l are used for the high performance liquid chromatography (HPLC) of camptothecine and measure.
Efficient liquid phase chromatographic analysis: adopt high performance liquid chromatograph (Waters company, the U.S.), comprise high-pressure pump (Waters2695) controller and a photodiode array detector (Waters2996).Chromatographic condition is: chromatographic column is used C-18 post (5 μ m, 4.6 * 250 millimeters), and wash-out moving phase acetonitrile: water (30: 70), flow velocity are 1ml/min, and the detection wavelength is 254nm.Retention time is 11.8min.Adopt peak area to carry out quantitatively.The camptothecine standard substance are available from Nat'l Pharmaceutical ﹠ Biological Products Control Institute.Sample repeats to average for three times.(following examples adopt the same detection method)
| Camptothecine content in the fruit (%/dry weight) | |
| Contrast tree 1 | 0.118±0.026 |
| Contrast tree 2 | 0.129±0.032 |
| Contrast tree 3 | 0.117±0.018 |
| Test group tree 1 | 0.182±0.044 |
| Test group tree 2 | 0.185±0.035 |
| Test group tree 3 | 0.176±0.037 |
Conclusion: the content that adopts foliage promoter provided by the invention camptothecine in fruit is than improving more than 40% without the use foliage promoter.
Embodiment 5 usefulness do not begin as a result the camplotheca acuminata earsh and test
Camplotheca acuminata treelet provenance is picked up from Guangzhou Tianhe District dragon's cave-stalactite cave Guangdong Institute of Traditional Chinese Medicine Chinese herbal medicine specimen garden, plants in research and teaching district, dragon's cave-stalactite cave Chinese herbal medicine specimen garden, Guangdong Province, field planting in 2005, the diameter of a cross-section of a tree trunk 1.3 meters above the ground reaches 4 centimetres, and highly about 5 meters, about 4 meters of crown diameter, it is good to grow, and branches and leaves are luxuriant.
On May 24th, 2008, from gathering branches and leaves the last weeks, the treelet of selected 6 identical growing way unanimities of the age of tree is divided into two groups (treatment group and control groups) at random.Treatment group sprays the foliage promoter of embodiment 2; Shortly before gathering branches and leaves June 1, spray embodiment 2 foliage promoters once more then.Spray dose squirting each face of branches and leaves and fruit, but drops physic liquor is not degree.Control group sprays clear water twice the same period.On November 1st, 2007, picking fruit, the indoor concealment in back of gathering is preserved moisture and was placed 3 days, and the nature of not preserving moisture then dries in the shade, and pulverizes.Measure camptothecine content (three repetitions) as stated above.
| Camptothecine content (%/dry weight) | |
| Contrast tree 1 | 0.103±0.021 |
| Contrast tree 2 | 0.102±0.015 |
| Contrast tree 3 | 0.104±0.016 |
| Experimental group tree 1 | 0.142±0.023 |
| Experimental group tree 2 | 0.144±0.018 |
| Experimental group tree 3 | 0.151±0.028 |
Conclusion: the content that adopts foliage promoter provided by the invention camptothecine in the treelet branches and leaves is than improving more than 40% without the use foliage promoter.
The camplotheca acuminata of embodiment 6 short close cultivation treelets
The provenance of the camplotheca acuminata of short close cultivation is picked up from Guangzhou Tianhe District dragon's cave-stalactite cave Guangdong Institute of Traditional Chinese Medicine Chinese herbal medicine specimen garden, plants in Guangdong Institute of Traditional Chinese Medicine living collection planting experiment district.Adopt the mode seeding and seedling raising broadcast sowing in January, 2008, application rate is every square 20 gram, sets up little shed heat and moisture preserving with film, treat seed germination after, the two ends of little shed are opened, and water every day and once promote growth.Before the sprigging, planting site is turned in advance, made 6 of wide 120 centimetres, high 15 centimetres furrow, length is decided with the plot.Long during when seedling to 15 centimetres of left and right sides, press 80*80 centimetre specification plantation, drench sufficient normal root water after the field planting, afterwards every day trickle once, regularly weed a garden, with the assurance well-grown.Treatment group picked at random 3 furrow are handled, and spray foliage promoter at twice: when Camptotheca acuminata seedlings begins to seal when capable, evenly spray branches and leaves by embodiment 3; Gather the eve at branches and leaves in one week back, repeat spraying once spray dose squirting each face of branches and leaves and fruit, but drops physic liquor is not degree again.And the branches and leaves of gathering at once.Other 3 furrow when Camptotheca acuminata seedlings begins to seal when capable, spray clear water in contrast, gather after the week eve, spray clear water more once.The indoor concealment in back of gathering is preserved moisture and was placed 3 days, and the nature of not preserving moisture then dries in the shade, and pulverizes.Measure camptothecine content (three times repeat to average) as stated above.
| Camptothecine content (%/dry weight) | |
| Contrast tree 1 | 0.107±0.023 |
| Contrast tree 2 | 0.109±0.027 |
| Contrast tree 3 | 0.106±0.018 |
| Test group tree 1 | 0.185±0.042 |
| Test group tree 2 | 0.177±0.046 |
| Test group tree 3 | 0.173±0.034 |
Conclusion: adopt foliage promoter of the present invention can make the average camptothecine content of short close cultivation branches and leaves increase more than 60%.
Claims (2)
1. foliage promoter that improves camptothecine content in the camplotheca acuminata is characterized in that the pulvis that foliage promoter is made by the weight part of following raw material:
10～12 parts of heavy metallic salts
5～10 parts of rare earths
1～5 part of organic acid
1～5 part of plant-growth regulator.
2. according to the said foliage promoter that improves camptothecine content in the camplotheca acuminata of claim 1, it is characterized in that said heavy metallic salt is copper sulfate or zinc sulfate, said rare earth is a rare earth chloride, said organic acid is propanedioic acid or oxysuccinic acid, and said plant-growth regulator is choline dichloride or choline chloride 60 or paclobutrazol or alar-85.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102491826A (en) * | 2011-11-11 | 2012-06-13 | 南京农业大学 | Multielement regulating foliage fertilizer for relieving water logging stress of soybeans and preparation method for multielement regulating foliage fertilizer |
| CN102674975A (en) * | 2012-05-29 | 2012-09-19 | 浙江农林大学 | Nutrition protective agent capable of promoting content of camptothecin in camptotheca acuminata to increase |
| CN108083863A (en) * | 2017-12-27 | 2018-05-29 | 江西省叶叶香农业开发有限公司 | The method that farm manure is prepared using rubbish from cooking |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1113224A (en) * | 1995-04-06 | 1995-12-13 | 厦门星科实业有限公司 | Plant growth-promoter and its producing method |
| CN1225163C (en) * | 2003-05-30 | 2005-11-02 | 陕西师范大学 | High-content camptothecine camplotheca acuminata callus culture method |
| CN1234264C (en) * | 2003-11-20 | 2006-01-04 | 东北林业大学 | Technology for culturing and collecting seeding of Camptotheca acuminata Decne |
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2009
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102491826A (en) * | 2011-11-11 | 2012-06-13 | 南京农业大学 | Multielement regulating foliage fertilizer for relieving water logging stress of soybeans and preparation method for multielement regulating foliage fertilizer |
| CN102674975A (en) * | 2012-05-29 | 2012-09-19 | 浙江农林大学 | Nutrition protective agent capable of promoting content of camptothecin in camptotheca acuminata to increase |
| CN108083863A (en) * | 2017-12-27 | 2018-05-29 | 江西省叶叶香农业开发有限公司 | The method that farm manure is prepared using rubbish from cooking |
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