CN101518184B - Method for improving content of various steroid saponins in rhizome of paris polyphylla var.yunnanensis - Google Patents
Method for improving content of various steroid saponins in rhizome of paris polyphylla var.yunnanensis Download PDFInfo
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- CN101518184B CN101518184B CN2009100614144A CN200910061414A CN101518184B CN 101518184 B CN101518184 B CN 101518184B CN 2009100614144 A CN2009100614144 A CN 2009100614144A CN 200910061414 A CN200910061414 A CN 200910061414A CN 101518184 B CN101518184 B CN 101518184B
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- methyl dihydrojasmonate
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Abstract
The invention discloses a method for improving the content of various steroid saponins in the rhizome of paris polyphylla var.yunnanensis. The method comprises the following steps: the methyl dihydrojThe invention discloses a method for improving the content of various steroid saponins in the rhizome of paris polyphylla var.yunnanensis. The method comprises the following steps: the methyl dihydrojasmonate is dissolved by using alcohol with concentration being higher than or equal to 75 percent according to the mass percent, and then water is added in for dilution to obtain methyl dihydrojasmonasmonate is dissolved by using alcohol with concentration being higher than or equal to 75 percent according to the mass percent, and then water is added in for dilution to obtain methyl dihydrojasmonate solution with concentration of 30 to 60mg/L; the methyl dihydrojasmonate solution is spayed on the fully-expanded leaves of the paris polyphylla var.yunnanensis without stopping until the methyl date solution with concentration of 30 to 60mg/L; the methyl dihydrojasmonate solution is spayed on the fully-expanded leaves of the paris polyphylla var.yunnanensis without stopping until the methyl dihydrojasmonate solution on the leaves drops once a year in July or August until the rhizoma of the paris polyphylla var.yunnanensis can be used as medicine. The method has the advantages of simple anihydrojasmonate solution on the leaves drops once a year in July or August until the rhizoma of the paris polyphylla var.yunnanensis can be used as medicine. The method has the advantages of simple and inexpensive medicine and the instrument equipment, thereby having less investment and low cost, being easy to implement and remarkably increasing the content of the saponin; and in addition, the metd inexpensive medicine and the instrument equipment, thereby having less investment and low cost, being easy to implement and remarkably increasing the content of the saponin; and in addition, the method is non-toxic to human and animals, has no pollution to the environment and has broad application prospect. hod is non-toxic to human and animals, has no pollution to the environment and has broad application prospect.
Description
Technical field
The present invention relates to a kind of method that improves multiple contents of steroid saponin in the plant materials subterraneous root shape stem portion, specifically, relate to a kind of method of utilizing methyl dihydrojasmonate to promote multiple contents of steroid saponin in the paris polyphylla rhizome.
Background technology
Rhizoma Paridis is the per nnial herb of Trilliaceae Paris, mainly is distributed in ground such as southwest China, particularly Yunnan, Sichuan and Guizhou.Rhizoma Paridis is famous and precious medicinal plant, and the steroid saponin compound that contains in its root stock mainly comprises pennogenin and dioscin, is its main active ingredient, has anticancer, hemostasis, anti-inflammatory, analgesia, relieving cough and asthma and immunomodulatory isoreactivity.The phenology characteristics that Rhizoma Paridis grows show: root stock terminal bud in 4～June unearthed (emerging), open up leaf, exhibition flower, pollen sac open (or leaf, flower, pollen sac are opened up simultaneously and put) subsequently, 5～July, growth stopped, 9～October cracking of fruit, 11～December over-ground part withered, fall seedling.The physiological characteristic of Rhizoma Paridis plant shows as: photosynthetic area is little, and a biennial seedling has only 1 leaf, 4～6 leaves is arranged in 4 years, also has only 5～11 pieces of blades after 5 years maturations; The blade efficiency of light energy utilization is low, a little less than the photosynthetic capacity; Vegetative period is short, only 6～7 months.
This shows that the annual biomass accumulation of Rhizoma Paridis is less, causing is used as medicine from the seedling to the root stock needs the time in 7～10 years at least.In recent years, along with market increasing rapidly to its demand, people are predatory wantonly in the open air to excavate, the artificial destruction of ecotope, Rhizoma Paridis resource distribution fragmentation, the breeding potential of Rhizoma Paridis own is low in addition etc., and factor has had a strong impact on its growth and procreation in the country of origin, cause Rhizoma Paridis progressively to step into the threatened plant ranks, the industrial medicinal raw material of Paris polyphylla occurs seriously in short supply.From the angle of implementing the strategy of sustainable development and preserving the Rhizoma Paridis natural resources, it is carried out the road that artificial culture is a certainty, the artificial domesticating cultivation that therefore carries out Rhizoma Paridis is extremely urgent.
Methyl dihydrojasmonate is the jasmonic analog derivative.Find that in the recent period the jasmine acid plays the signal mediation in the multiple adverse circumstance reaction of vegetable cell, cause the expression of the degeneration-resistant reaction product of cell, multiple secondary metabolite synthetic had inducing action, be considered to very effective elicitor.
Do not occur at present utilizing methyl dihydrojasmonate to improve the report of contents of steroid saponin in the paris polyphylla rhizome part as yet.
Summary of the invention
The objective of the invention is to remedy the deficiencies in the prior art, a kind of method that improves multiple contents of steroid saponin in the paris polyphylla rhizome is provided.
For achieving the above object, the present invention adopts following technical scheme: a kind of method that improves multiple contents of steroid saponin in the paris polyphylla rhizome may further comprise the steps:
(1) alcohol with mass percent concentration 〉=75% dissolves methyl dihydrojasmonate, and thin up is made into the methyl dihydrojasmonate solution that concentration is 30～60mg/L then;
(2) 7～August of a calendar year to the exhibition leaf completely the Rhizoma Paridis blade evenly spray methyl dihydrojasmonate solution one time, the methyl dihydrojasmonate solution on blade stops to spray in the time of will dripping.
Be that alcohol with mass percent concentration 〉=75% dissolves methyl dihydrojasmonate in the above-mentioned steps (1), thin up is made into the methyl dihydrojasmonate solution that concentration is 45mg/L then.
Be in the above-mentioned steps (2) the dusk time-division, to the exhibition leaf completely the Rhizoma Paridis blade evenly spray methyl dihydrojasmonate solution one time.
In the above-mentioned steps (2) be to the exhibition leaf completely the tow sides of Rhizoma Paridis blade evenly spray methyl dihydrojasmonate solution one time.
In the above-mentioned steps (2) be 7～August in every year to the exhibition leaf completely the Rhizoma Paridis blade evenly spray methyl dihydrojasmonate solution one time, can be used as medicine until its root stock.
Experimental result shows, among the present invention in every year the 7～August in the dusk time-division, to the exhibition leaf blade tow sides of Rhizoma Paridis plant the methyl dihydrojasmonate solution that evenly to spray concentration be 45mg/L completely, can be used as medicine until its root stock, can reach best effect.
The present invention has the following advantages:
(1) required medicine, plant and instrument simple cheap, so less investment, cost is low.
(2) implementation process is simple, make then give birth to and life in former years root stock in total saponin content improved 15% and 38% respectively, to people and animals' toxicological harmless, environment is not had influence.This method has great importance for the output and the quality that improve the Rhizoma Paridis field planting.
Embodiment
The invention will be further described below by specific embodiment.
Embodiment 1
Alcohol with mass percent concentration 〉=75% dissolves methyl dihydrojasmonate, the alcohol consumption can dissolve methyl dihydrojasmonate fully and gets final product, thin up is made into the methyl dihydrojasmonate solution that concentration is 30mg/L again, in in every year the 7～August in the dusk time-division, Rhizoma Paridis blade tow sides are evenly sprayed methyl dihydrojasmonate solution, other gets the water with the above-mentioned amount of spraying equivalent, blade tow sides on the Rhizoma Paridis plant that does not spray methyl dihydrojasmonate solution are evenly sprayed, with in contrast.Spray every year once, can be used as medicine up to its root stock.In case of rainy, can fill spray.Treat that Rhizoma Paridis falls behind the seedling, detects contents of steroid saponin in the root stock with the RP-HPLC method.Calculate its give birth to then and life in former years root stock in total saponin content rate of increase be respectively 10% and 26%.
Embodiment 2
Adopt with embodiment 1 similar methods to experimentize, it is that the methyl dihydrojasmonate solution of 45mg/L sprays that difference is to adopt concentration.Calculate its give birth to then and life in former years root stock in total saponin content rate of increase be respectively 15% and 38%.
Embodiment 3
Adopt with embodiment 1 similar methods to experimentize, it is that the methyl dihydrojasmonate solution of 60mg/L sprays that difference is to adopt concentration.Calculate its give birth to then and life in former years root stock in total saponin content rate of increase be respectively 10% and 11%.
The inventive method effect is remarkable, for increasing contents of steroid saponin in the paris polyphylla rhizome, improving the approach that the root stock quality provides a simple possible.
Claims (5)
1. method that improves multiple contents of steroid saponin in the paris polyphylla rhizome is characterized in that may further comprise the steps:
(1) alcohol with mass percent concentration 〉=75% dissolves methyl dihydrojasmonate, and thin up is made into the methyl dihydrojasmonate solution that concentration is 30～60mg/L then;
(2) 7～August of a calendar year to the exhibition leaf completely the Rhizoma Paridis blade evenly spray methyl dihydrojasmonate solution one time, the methyl dihydrojasmonate solution on blade stops to spray in the time of will dripping.
2. the method for multiple contents of steroid saponin in the raising paris polyphylla rhizome according to claim 1, it is characterized in that: be that alcohol with mass percent concentration 〉=75% dissolves methyl dihydrojasmonate in the step (1), thin up is made into the methyl dihydrojasmonate solution that concentration is 45mg/L then.
3. the method for multiple contents of steroid saponin in the raising paris polyphylla rhizome according to claim 1 is characterized in that: be in the step (2) the dusk time-division, to the exhibition leaf completely the Rhizoma Paridis blade evenly spray methyl dihydrojasmonate solution one time.
4. according to the method for multiple contents of steroid saponin in claim 1 or the 3 described raising paris polyphylla rhizomes, it is characterized in that: in the step (2) be to the exhibition leaf completely the tow sides of Rhizoma Paridis blade evenly spray methyl dihydrojasmonate solution one time.
5. the method for multiple contents of steroid saponin in the raising paris polyphylla rhizome according to claim 4, it is characterized in that: in the step (2) be 7～August in every year to the exhibition leaf completely the Rhizoma Paridis blade evenly spray methyl dihydrojasmonate solution one time, can be used as medicine until its root stock.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1210663A (en) * | 1998-09-08 | 1999-03-17 | 中国科学院昆明植物研究所 | Seed sprouting and quick propagating technology of Yunnan Rhizoma Paridis |
| CN101142895A (en) * | 2007-10-18 | 2008-03-19 | 云南省农业科学院高山经济植物研究所 | Method for selecting and breeding paris yunnanensis franch cultivated species |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1210663A (en) * | 1998-09-08 | 1999-03-17 | 中国科学院昆明植物研究所 | Seed sprouting and quick propagating technology of Yunnan Rhizoma Paridis |
| CN101142895A (en) * | 2007-10-18 | 2008-03-19 | 云南省农业科学院高山经济植物研究所 | Method for selecting and breeding paris yunnanensis franch cultivated species |
Non-Patent Citations (3)
| Title |
|---|
| 何俊 等.滇重楼植物的研究进展.《云南植物研究 》.2006,第28卷(第3期),271-276. * |
| 杨斌 等.滇重楼的栽培与合理利用.《中国野生植物资源》.2008,第27卷(第6期),70-73. * |
| 陈昌祥 等.滇重楼地上部分的甾体皂甙.《云南植物研究》.1990,第12卷(第3期),323-329. * |
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