CN101518185B - Method for improving content of various steroid saponins in Paris polyphylla var.yunnanensis plants - Google Patents
Method for improving content of various steroid saponins in Paris polyphylla var.yunnanensis plants Download PDFInfo
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- CN101518185B CN101518185B CN2009100614229A CN200910061422A CN101518185B CN 101518185 B CN101518185 B CN 101518185B CN 2009100614229 A CN2009100614229 A CN 2009100614229A CN 200910061422 A CN200910061422 A CN 200910061422A CN 101518185 B CN101518185 B CN 101518185B
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Abstract
The invention discloses a method for improving the content of various steroid saponins in Paris polyphylla var.yunnanensis plants. The method comprises the following steps: the aminozide, the (2-ChlorThe invention discloses a method for improving the content of various steroid saponins in Paris polyphylla var.yunnanensis plants. The method comprises the following steps: the aminozide, the (2-Chlorively improved by 11 percent, 26 percent and 25 percent, and the required medicine and the instrument equipment are simple and inexpensive, therefore, the investment is less and the cost is low; andctively improved by 11 percent, 26 percent and 25 percent, and the required medicine and the instrument equipment are simple and inexpensive, therefore, the investment is less and the cost is low; and in addition, the implementation process is simple and feasible, and the method is non-toxic to human and animals and has no pollution to the environment.in addition, the implementation process is simple and feasible, and the method is non-toxic to human and animals and has no pollution to the environment.oethyl) trimethylammonium chloride or the mepiquat chloride is dissolved in water to be prepared into aminozide solution with concentration of 50-500mg*L<-1>, (2-Chloroethyl) trimethylammonium chloridoethyl) trimethylammonium chloride or the mepiquat chloride is dissolved in water to be prepared into aminozide solution with concentration of 50-500mg*L<-1>, (2-Chloroethyl) trimethylammonium chloride solution with concentration of 39-2500mg*L<-1> or mepiquat chloride solution with concentration of 0.125-0.5mg*g<-1>; and the aminozide solution, the (2-Chloroethyl) trimethylammonium chloride solute solution with concentration of 39-2500mg*L<-1> or mepiquat chloride solution with concentration of 0.125-0.5mg*g<-1>; and the aminozide solution, the (2-Chloroethyl) trimethylammonium chloride solution or the mepiquat chloride solution is evenly spayed on the fully expanded leaves of the Paris polyphylla var.yunnanensis without stopping until the solution on the leaves drops once a year in Julyion or the mepiquat chloride solution is evenly spayed on the fully expanded leaves of the Paris polyphylla var.yunnanensis without stopping until the solution on the leaves drops once a year in Julyor August until the rhizoma of the paris polyphylla var.yunnanensis can be used as medicine. By adopting the method, the content of the steroid saponin in the Paris polyphylla var.yunnanensis is respeor August until the rhizoma of the paris polyphylla var.yunnanensis can be used as medicine. By adopting the method, the content of the steroid saponin in the Paris polyphylla var.yunnanensis is respect
Description
Technical field
The present invention relates to a kind of method that improves multiple contents of steroid saponin in the plant corpus, specifically, relate to a kind of method of utilizing the external source plant growth regulator to promote multiple contents of steroid saponin in the Yunnan Rhizoma Paridis plant corpus.
Background technology
Yunnan Rhizoma Paridis is the herbaceos perennial of Trilliaceae Paris, mainly is distributed in ground such as southwest China, particularly Yunnan, Sichuan and Guizhou.Yunnan Rhizoma Paridis is famous and precious medicinal plant, and the steroid saponin compound that contains in its root-like stock mainly comprises pennogenin and Dioscin, is its main active, has anticancer, hemostasis, anti-inflammatory, analgesia, relieving cough and asthma and immunological regulation isoreactivity.In recent years, along with market increasing rapidly to its demand, people are predatory wantonly in the open air to excavate, the artificial destruction of ecotope, Yunnan Rhizoma Paridis resource distribution fragmentation, the reproduction rate of Yunnan Rhizoma Paridis own is low in addition etc., and factor has had a strong impact on its growth and procreation in the original producton location, cause Yunnan Rhizoma Paridis progressively to step into the endangered plants ranks, the industrial medicinal raw material of Paris polyphylla occurs seriously in short supply.From the angle of implementing the strategy of sustainable development and preserving the Yunnan Rhizoma Paridis natural resources, it is carried out the road that artificial cultivation is a certainty, the artificial domesticating cultivation that therefore carries out Yunnan Rhizoma Paridis is extremely urgent.
The phenology characteristics that Yunnan Rhizoma Paridis grows show: root-like stock terminal bud in 4～June unearthed (emerging), open up leaf, exhibition flower, pollen sac open (or leaf, flower, pollen sac are opened up simultaneously and put) subsequently, 5～July, growth stopped, 9～October cracking of fruit, 11～December acrial part withered, fall seedling.The physiological characteristic of Yunnan Rhizoma Paridis plant shows as: photosynthetic area is little, and a biennial seedling has only 1 leaf, 4～6 leaves is arranged in 4 years, also has only 5～11 pieces of blades after 5 years maturations; The blade utility rate of luminous energy is low, a little less than the photosynthetic capacity; Vegetative period is short, only 6～7 months.
This shows that the annual biomass accumulation of Yunnan Rhizoma Paridis is less, causing is used as medicine from the seedling to the root-like stock needs the time in 7～10 years at least, and quality of medicinal material has much room for improvement.For improving this situation, the method for studying a kind of simple possible improves contents of steroid saponin in the Yunnan Rhizoma Paridis, has important effect for the artificial domesticating cultivation of Yunnan Rhizoma Paridis.
Summary of the invention
The objective of the invention is to remedy the deficiencies in the prior art, a kind of method that improves multiple contents of steroid saponin in the Yunnan Rhizoma Paridis plant corpus is provided.
For achieving the above object, the present invention adopts following technical scheme: a kind of method that improves multiple contents of steroid saponin in the Yunnan Rhizoma Paridis plant corpus may further comprise the steps:
(1) daminozide or 2-chloroethyl trimethyl ammonium chloride or chlormequat chloride is soluble in water, being mixed with concentration is 50～500mgL
-1Daminozide solution, or concentration is 39～2500mgL
-12-chloroethyl trimethyl ammonium chloride solution, or concentration is 0.125～0.5mgg
-1Chlormequat chloride solution;
(2) 7～August of a calendar year to the exhibition leaf completely the Yunnan Rhizoma Paridis blade evenly spray daminozide solution or 2-chloroethyl trimethyl ammonium chloride solution or chlormequat chloride solution, the solution on blade stops to spray in the time of will dripping.
Be that daminozide or 2-chloroethyl trimethyl ammonium chloride or chlormequat chloride is soluble in water in the above-mentioned steps (1), being mixed with concentration is 500mgL
-1Daminozide solution or concentration be 625mgL
-12-chloroethyl trimethyl ammonium chloride solution, or concentration is 0.125mgg
-1Chlormequat chloride solution.
Be in the above-mentioned steps (2) the dusk time-division, to the exhibition leaf completely the Yunnan Rhizoma Paridis blade evenly spray daminozide solution or 2-chloroethyl trimethyl ammonium chloride solution or chlormequat chloride solution.
In the above-mentioned steps (2) be to the exhibition leaf completely the tow sides of Yunnan Rhizoma Paridis blade evenly spray daminozide solution or 2-chloroethyl trimethyl ammonium chloride solution or chlormequat chloride solution.
In the above-mentioned steps (2) be 7～August in every year to the exhibition leaf completely the Yunnan Rhizoma Paridis blade evenly spray daminozide solution or 2-chloroethyl trimethyl ammonium chloride solution or chlormequat chloride solution, can be used as medicine until its root-like stock.
Experimental result shows that adopting concentration among the present invention is 500mgL
-1Daminozide solution, or concentration is 625mgL
-12-chloroethyl trimethyl ammonium chloride solution, or concentration is 0.125mgg
-1Chlormequat chloride solution, in every year the 7～August in the dusk time-division, to the exhibition leaf completely the blade tow sides of Yunnan Rhizoma Paridis plant evenly spray once, can be used as medicine until its root-like stock, can reach optimum efficiency.
The present invention has following advantage:
1. daminozide, 2-chloroethyl trimethyl ammonium chloride and chlormequat chloride are the plant growth regulator of having many uses, under suitable concentration, they are sprayed respectively on the Yunnan Rhizoma Paridis plant, can regulate plant strain growth, optimize plant type, make plant solid resistant to lodging, improve plant root-like stock output, and can improve the utility rate of luminous energy of blade, increase contents of steroid saponin in the Yunnan Rhizoma Paridis.Adopt above-mentioned three kinds of solution among the present invention, make that contents of steroid saponin has improved 11%, 26% and 25% respectively in the Yunnan Rhizoma Paridis;
2. required medicine, instrument and equipment simple cheap, so less investment, cost is low;
3. implementation process is simple, can significantly improve contents of steroid saponin, to people and animals' nonhazardous, environment is not had influence.
Embodiment
The invention will be further described below by specific embodiment, and its purpose is to help to understand content of the present invention better, but the protection domain that these specific embodiments do not limit the present invention in any way.
Embodiment 1
Be mixed with that concentration is 50,500mgL with daminozide is water-soluble
-1Solution, in every year the 7～August in dusk time-division to the exhibition leaf completely the tow sides of Yunnan Rhizoma Paridis blade evenly spray once, the daminozide solution on blade stops to spray in the time of will dripping.Other gets respectively the water with the above-mentioned amount of spraying equivalent, and the blade tow sides of adopting in the same way the Yunnan Rhizoma Paridis plant that do not spray daminozide solution evenly spray once, with in contrast.In case of rainy, can fill spray.The Yunnan Rhizoma Paridis plant of waiting to spray water and spraying daminozide solution falls behind the seedling, detects the content of steroid saponin in the Yunnan Rhizoma Paridis plant respectively with the RP-HPLC method, contrasts with the Yunnan Rhizoma Paridis that sprays water, and the result shows and sprays 500mgL
-1The paris polyphylla rhizome pennogenin content of daminozide solution has improved 14%, sprays 50,500mgL
-1The paris polyphylla rhizome Dioscin content of daminozide solution improved 9%, 7% respectively, make total steroid saponin content improve 10%, 11% respectively.
Embodiment 2
Experimentize according to the method identical with embodiment 1, difference is to adopt that concentration is 10,39,625,2500mgL
-12-chloroethyl trimethyl ammonium chloride solution spray.Paris polyphylla rhizome pennogenin content has improved 55%, 12%, 47%, 37% respectively, sprays 625mgL
-1The paris polyphylla rhizome Dioscin content of 2-chloroethyl trimethyl ammonium chloride solution has improved 23%, makes total steroid saponin content improve 26%.
Embodiment 3
Experimentize according to the method identical with embodiment 1, difference is to adopt that concentration is 0.125,0.25,0.5mgg
-1Chlormequat chloride solution spray.Paris polyphylla rhizome pennogenin content has improved 80%, 21%, 49% respectively, and root-like stock Dioscin content has improved 18%, 1%, 3% respectively, and total steroid saponin content has improved 25%, 5%, 8% respectively.
Claims (5)
1. method that improves multiple contents of steroid saponin in the paris polyphylla rhizome is characterized in that may further comprise the steps:
(1) alcohol with mass percent concentration 〉=75% dissolves MDJ, and thin up is made into the MDJ solution that concentration is 30～60mg/L then;
(2) 7～August of a calendar year to the exhibition leaf completely the Yunnan Rhizoma Paridis blade evenly spray MDJ solution one time, the MDJ solution on blade stops to spray in the time of will dripping.
2. the method for multiple contents of steroid saponin in the raising paris polyphylla rhizome according to claim 1, it is characterized in that: be that alcohol with mass percent concentration 〉=75% dissolves MDJ in the step (1), thin up is made into the MDJ solution that concentration is 45mg/L then.
3. the method for multiple contents of steroid saponin in the raising paris polyphylla rhizome according to claim 1 is characterized in that: be in the step (2) the dusk time-division, to the exhibition leaf completely the Yunnan Rhizoma Paridis blade evenly spray MDJ solution one time.
4. according to the method for multiple contents of steroid saponin in each described raising paris polyphylla rhizome in the claim 1～3, it is characterized in that: in the step (2) be to the exhibition leaf completely the tow sides of Yunnan Rhizoma Paridis blade evenly spray MDJ solution one time.
5. according to the method for multiple contents of steroid saponin in each described raising paris polyphylla rhizome in the claim 1～3, it is characterized in that: in the step (2) be 7～August in every year to the exhibition leaf completely the Yunnan Rhizoma Paridis blade evenly spray MDJ solution one time, can be used as medicine until its root-like stock.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100614229A CN101518185B (en) | 2009-04-03 | 2009-04-03 | Method for improving content of various steroid saponins in Paris polyphylla var.yunnanensis plants |
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| CN2009100614229A CN101518185B (en) | 2009-04-03 | 2009-04-03 | Method for improving content of various steroid saponins in Paris polyphylla var.yunnanensis plants |
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| CN101518185A CN101518185A (en) | 2009-09-02 |
| CN101518185B true CN101518185B (en) | 2010-11-10 |
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