CN105503358A - Fertilizer for increasing polysaccharide content in noble dendrobium and applying method thereof - Google Patents

Fertilizer for increasing polysaccharide content in noble dendrobium and applying method thereof Download PDF

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Publication number
CN105503358A
CN105503358A CN201610093557.3A CN201610093557A CN105503358A CN 105503358 A CN105503358 A CN 105503358A CN 201610093557 A CN201610093557 A CN 201610093557A CN 105503358 A CN105503358 A CN 105503358A
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China
Prior art keywords
dendrobium
fertilizer
stem
noble dendrobium
special fertilizer
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CN201610093557.3A
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Chinese (zh)
Inventor
龚庆芳
何金祥
黄宁珍
付传明
冼康华
苏江
黄惠锦
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Guangxi Institute of Botany of CAS
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Guangxi Institute of Botany of CAS
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Priority to CN201610093557.3A priority Critical patent/CN105503358A/en
Publication of CN105503358A publication Critical patent/CN105503358A/en
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B7/00Fertilisers based essentially on alkali or ammonium orthophosphates
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C21/00Methods of fertilising, sowing or planting
    • A01C21/005Following a specific plan, e.g. pattern
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D9/00Other inorganic fertilisers
    • C05D9/02Other inorganic fertilisers containing trace elements

Abstract

The invention discloses a fertilizer for increasing the polysaccharide content in noble dendrobium and an applying method thereof. The fertilizer for increasing the polysaccharide content in the noble dendrobium comprises a special fertilizer A and a special fertilizer B, wherein the special fertilizer A is an aqueous solution of monopotassium phosphate, boric acid and zinc sulfate heptahydrate and is prepared from 0.2-4 g/L of monopotassium phosphate, 5-15 mg/L of boric acid, 10-40 mg/L of zinc sulfate heptahydrate and the balance water, and the special fertilizer B is an aqueous solution of sodium selenite, sodium molybdate and manganese sulfate monohydrate and is prepared from 0.001-0.1 mg/L of sodium selenite, 0.2-0.6 mg/L of sodium molybdate, 10-40 mg/L of manganese sulfate monohydrate and the balance water. The fertilizer can effectively promote growth of the noble dendrobium especially medicinal stems of noble dendrobium and greatly increase the content of polysaccharide and mannose in the noble dendrobium especially medicinal stems of noble dendrobium.

Description

Improve fertilizer and the application process thereof of polysaccharide content in the stem of noble dendrobium
Technical field
The present invention relates to the fertilizer improving polysaccharide content in medicinal plant, be specifically related to a kind of fertilizer and the application process thereof that improve polysaccharide content in the stem of noble dendrobium.
Background technology
The stem of noble dendrobium, one of the orchid family per nnial herb, the world has orchid 700 to belong to nearly 2000 kinds, and China has 170 genus more than 1200 to plant.There is the stem of noble dendrobium 74 kinds in China, has 40 kinds of stems of noble dendrobium to have pharmaceutical use in the world.Mainly be distributed in Tropical Asian and subtropics, Australia and Pacific Islands, China's major part is distributed in the ground such as southwest, south China, Taiwan.The principal item of dendrobium has Herba Dendrobii, dendrobium stem, purple dendrobium, Dendrobium chrysotoxum etc., can be used as medicine, and the stem of noble dendrobium by name, drives solution abnormal heat to human body, the curative effects such as beneficial smart reinforcing yin essence.
Herba Dendrobii (Dendrobiumofficinale) is the perennial herbaceous plant that grows nonparasitically upon another plant of the orchid family Dendrobium, because its eustipes part is chocolate, has another name called Herba hedyotis costatae.Its fresh or dry stem uses as medicinal material, and be rare crude drugs, medicinal history is long, is classified as top grade in Shennong's Herbal, has nourishing Yin and clearing heat, and promote the production of body fluid beneficial stomach, and the effect such as moisten the lung and relieve the cough.Herba Dendrobii requires harsher to growing environment, needs special ecological environmental condition, and Herba Dendrobii wild resource is few, within 1987, is classified as one of animals and plants medicinal material of dying out in imminent danger by country.
Containing polysaccharide, seminose, alkaloid, lymphatic temperament and various trace elements in Herba Dendrobii.Polyose composition is activeconstituents (Li Manfei etc., herbal medicine, 1990 in Herba Dendrobii with immuno-potentiation and antitumor action,, and content higher (yellow civil rights etc., CHINA JOURNAL OF CHINESE MATERIA MEDICA 21 (10): 10), 1997,22 (2): 74).Polysaccharide and mannose content are the Main Basiss that " Chinese Pharmacopoeia " 2010 editions regulation evaluates Herba Dendrobii quality, and wherein the polysaccharide of regulation Herba Dendrobii is in dextrose anhydrous, and content must not be less than 25%; 13.0-38.0% is should be containing seminose.Visible, the quality of how many direct reflection Herba Dendrobii qualities of polysaccharide and seminose in Herba Dendrobii, the content being improved polysaccharide and seminose in Herba Dendrobii by cultivation management method is had great importance for the Herba Dendrobii of production high-quality.
Publication number is that the patent of invention of CN103704035A discloses a kind of cultivating method improving Dendrobium officinale polysaccharide class and phenols component, comprise and in the course of cultivation UVB radiation is manually increased to Herba Dendrobii, the content of polyose and phenols component in Herba Dendrobii stem can be made to improve 23.1-55.6% and 9.8-65.0% respectively.But, existing result of study shows, increased UV-B radiation can cause blade to produce excess activity oxygen molecule, active oxygen can react with many cellular components, thus cause enzyme deactivation, photosynthetic pigments and lipid peroxidation etc. (Jiang Gaoming. plant physioecology [M]. Beijing: Higher Education Publishing House, 2004:170-178).
Publication number is that the patent of invention of CN102172152A discloses a kind of method improving Dendrobium officinale polysaccharide, concrete employing following methods is cultivated: the growth Herba Dendrobii seedling of 2-3 month is carried out greenhouse cultivation, plant line-spacing is 25-45cm, spacing in the rows is 35-45cm, continue to adopt Light To Dark Ratio to be that 12-15h:2-5h carries out interval light photograph, temperature of shed controls at 19-28 DEG C, and sprayed foliage fertilizer 0.02-0.05% potassium primary phosphates or 3-7% rich Jiamei bamboo vinegar foliage fertilizer once every 16-18 days, watered once permeable every 4-7 days, simultaneously with sound intensity 100-120 decibel, sound wave every day of frequency 950-1100 hertz 1-3 time, sound stimulation is stopped after within each 1-3 hour, carrying out continued stimulus 20-30 days, and combine control flower bud and manually pluck flower bud method.Though this invention can improve the polysaccharide content in Herba Dendrobii by a relatively large margin, but method is more complicated, operate more loaded down with trivial details.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of fertilizer and the application process thereof that improve polysaccharide content in the stem of noble dendrobium.Adopt fertilizer of the present invention and method to use medicinal dendrobium, effectively can improve the polysaccharide in the stem of noble dendrobium and mannose content.
The fertilizer of polysaccharide content in the raising stem of noble dendrobium of the present invention, comprises special fertilizer A and special fertilizer B, wherein:
Described special fertilizer A is the aqueous solution of potassium primary phosphate, boric acid and Zinc Sulphate Heptahydrate, specifically consists of: potassium primary phosphate 0.2-4g/L, and boric acid 5-15mg/L, Zinc Sulphate Heptahydrate 10-40mg/L, surplus are water;
Described special fertilizer B is the aqueous solution of Sodium Selenite, Sodium orthomolybdate and manganese sulfate monohydrate, specifically consists of: Sodium Selenite 0.001-0.1mg/L, Sodium orthomolybdate 0.2-0.6mg/L, manganese sulfate monohydrate 10-40mg/L, surplus are water.
In technique scheme, the concrete composition of described special fertilizer A is preferably: potassium primary phosphate 2-3.5g/L, boric acid 6-12mg/L, Zinc Sulphate Heptahydrate 10-30mg/L, surplus are water.The concrete composition of described special fertilizer B is preferably: Sodium Selenite 0.01-0.05mg/L, Sodium orthomolybdate 0.25-0.5mg/L, manganese sulfate monohydrate 10-30mg/L, surplus are water.
In technique scheme, the described stem of noble dendrobium is medicinal dendrobium, is preferably Herba Dendrobii, dendrobium stem, purple dendrobium or Dendrobium chrysotoxum in medicinal dendrobium.When the stem of noble dendrobium is Herba Dendrobii, adopt fertilizer of the present invention and method to use it, the polysaccharide in Herba Dendrobii and the raising of mannose content especially remarkable.
Adopt above-mentioned fertilizer to improve the application process of polysaccharide content in the stem of noble dendrobium, comprising: in the annual 4-6 month, monthly spray special fertilizer A once to next year life or the perennial stem of noble dendrobium, each amount of application is 400-600ml/m 2; And in the annual 10-12 month, monthly spray special fertilizer B once to next year life or the perennial stem of noble dendrobium, each amount of application is 400-600ml/m 2.
In above-mentioned application process, each amount of application of described special fertilizer A is preferably 500ml/m 2, each amount of application of described special fertilizer B is preferably 500ml/m 2.
In above-mentioned application process, the described stem of noble dendrobium is medicinal dendrobium, is preferably Herba Dendrobii, dendrobium stem, purple dendrobium or Dendrobium chrysotoxum in medicinal dendrobium.Described medicinal dendrobium can be Herba Dendrobii, dendrobium stem and Dendrobium chrysotoxum etc.It is raw that seedling age as the stem of noble dendrobium of subject is at least next year, also can be perennial.
Compared with prior art, the present invention adopts the supply source of potassium phosphate fertilizer and healthy trace elements with household boron, zinc, selenium, manganese and molybdenum to combine by specific proportioning and obtains special fertilizer A and special fertilizer B, use in vegetative growth phase of the stem of noble dendrobium especially medicinal dendrobium and polysaccharide accumulation phase simultaneously, thus effectively promote the growth of the stem of noble dendrobium especially medicinal dendrobium, and increase substantially the stem of noble dendrobium especially content of polysaccharide and seminose in medicinal dendrobium.Fertilizer of the present invention and application process especially remarkable to the effect of Herba Dendrobii.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in further detail, and to understand content of the present invention better, but the present invention is not limited to following examples.
Embodiment 1
Special fertilizer A consists of: potassium primary phosphate 2.0g/L, and boric acid 6mg/L, Zinc Sulphate Heptahydrate 10mg/L, surplus are water;
Special fertilizer B consists of: Sodium Selenite 0.01mg/L, Sodium orthomolybdate 0.25mg/L, manganese sulfate monohydrate 10mg/L, surplus are water.
Embodiment 2
Special fertilizer A consists of: potassium primary phosphate 3.0g/L, and boric acid 10mg/L, Zinc Sulphate Heptahydrate 20mg/L, surplus are water;
Special fertilizer B consists of: Sodium Selenite 0.02mg/L, Sodium orthomolybdate 0.35mg/L, manganese sulfate monohydrate 20mg/L, surplus are water.
Embodiment 3
Special fertilizer A consists of: potassium primary phosphate 3.5g/L, and boric acid 12mg/L, Zinc Sulphate Heptahydrate 30mg/L, surplus are water;
Special fertilizer B consists of: Sodium Selenite 0.05mg/L, Sodium orthomolybdate 0.5mg/L, manganese sulfate monohydrate 30mg/L, surplus are water.
Embodiment 4
Special fertilizer A consists of: potassium primary phosphate 4.0g/L, and boric acid 5mg/L, Zinc Sulphate Heptahydrate 40mg/L, surplus are water;
Special fertilizer B consists of: Sodium Selenite 0.08mg/L, Sodium orthomolybdate 0.6mg/L, manganese sulfate monohydrate 20mg/L, surplus are water.
Embodiment 5
Special fertilizer A consists of: potassium primary phosphate 2.5g/L, and boric acid 15mg/L, Zinc Sulphate Heptahydrate 35mg/L, surplus are water;
Special fertilizer B consists of: Sodium Selenite 0.1mg/L, Sodium orthomolybdate 0.2mg/L, manganese sulfate monohydrate 40mg/L, surplus are water.
Experimental example 1: applicant carries out in 2014 to 2015 planting bases in Guangxi Plant Inst., Chinese Academy of Sciences, Guangxi Zhuang Autonomous the test improving polysaccharide and seminose in Herba Dendrobii.
With the Herba Dendrobii plant of normal management, seedling age life in 2 years (kind is that osmanthus plants 5, and plant line-spacing is 10cm, and spacing in the rows is 10cm) for fertilising object, be divided into 4 pieces of pilot regions, every block pilot region 2m 2.
First piece of pilot region: respectively at the special fertilizer A respectively sprayed on May 5th, 2014, June 5 and July 5 described in embodiment 1 once, each amount of application is 500ml/m 2; Respectively spray special fertilizer B described in embodiment 1 once in September 20 the same year, October 20 and November 20, each amount of application is 500ml/m 2.
Second piece of pilot region: respectively at the special fertilizer A respectively sprayed on May 5th, 2014, June 5 and July 5 described in embodiment 2 once, each amount of application is 500ml/m 2; Respectively spray special fertilizer B described in embodiment 2 once in September 20 the same year, October 20 and November 20, each amount of application is 500ml/m 2.
3rd piece of pilot region: respectively at the special fertilizer A respectively sprayed on May 5th, 2014, June 5 and July 5 described in embodiment 3 once, each amount of application is 500ml/m 2; Respectively spray special fertilizer B described in embodiment 3 once in September 20 the same year, October 20 and November 20, each amount of application is 500ml/m 2.
4th piece of pilot region: be control group, daily spray clear water on May 5th, 2014, June 5 and July 5 and September 20 the same year, October 20 and November 20, each amount of application is 500ml/m 2.
In the field management of above-mentioned four pieces of pilot regions, except the above-mentioned applying fertilizer pointed out is different, other management is all identical.Four pieces of pilot regions, all in the fresh bar of Herba Dendrobii of gathering on March 23rd, 2015, calculate output, and the detection method provided according to 2010 " Chinese Pharmacopoeia " detects polysaccharide wherein and mannose content, result as described in Table 1:
Table 1
Pilot region Polysaccharide content % Mannose content % Output (fresh bar) kg/M 2
First piece 41.620 21.0 1.84
Second piece 44.355 20.0 2.0
3rd piece 41.090 21.7 2.12
4th piece 22.687 15.8 1.36
Result shows, and after Herba Dendrobii sprays fertilizer of the present invention, polysaccharide, mannose content improve 81.15-95.5% respectively, 26.58-37.34%.

Claims (10)

1. improve the fertilizer of polysaccharide content in the stem of noble dendrobium, comprise special fertilizer A and special fertilizer B, it is characterized in that:
Described special fertilizer A is the aqueous solution of potassium primary phosphate, boric acid and Zinc Sulphate Heptahydrate, specifically consists of: potassium primary phosphate 0.2-4g/L, and boric acid 5-15mg/L, Zinc Sulphate Heptahydrate 10-40mg/L, surplus are water;
Described special fertilizer B is the aqueous solution of Sodium Selenite, Sodium orthomolybdate and manganese sulfate monohydrate, specifically consists of: Sodium Selenite 0.001-0.1mg/L, Sodium orthomolybdate 0.2-0.6mg/L, manganese sulfate monohydrate 10-40mg/L, surplus are water.
2. the fertilizer of polysaccharide content in the raising stem of noble dendrobium according to claim 1, is characterized in that:
Described special fertilizer A specifically consists of: potassium primary phosphate 2-3.5g/L, and boric acid 6-12mg/L, Zinc Sulphate Heptahydrate 10-30mg/L, surplus are water.
3. the fertilizer of polysaccharide content in the raising stem of noble dendrobium according to claim 1, is characterized in that:
Described special fertilizer B specifically consists of: Sodium Selenite 0.01-0.05mg/L, Sodium orthomolybdate 0.25-0.5mg/L, manganese sulfate monohydrate 10-30mg/L, surplus are water.
4. the fertilizer of polysaccharide content in the raising stem of noble dendrobium according to any one of claim 1-3, is characterized in that: the described stem of noble dendrobium is medicinal dendrobium.
5. the fertilizer of polysaccharide content in the raising stem of noble dendrobium according to claim 4, is characterized in that: described medicinal dendrobium is Herba Dendrobii, dendrobium stem, purple dendrobium or Dendrobium chrysotoxum.
6. adopt fertilizer described in claim 1 to improve the application process of polysaccharide content in the stem of noble dendrobium, it is characterized in that: in the annual 4-6 month, monthly spray special fertilizer A once to next year life or the perennial stem of noble dendrobium, each amount of application is 400-600ml/m 2; And in the annual 10-12 month, monthly spray special fertilizer B once to next year life or the perennial stem of noble dendrobium, each amount of application is 400-600ml/m 2.
7. application process according to claim 6, is characterized in that: each amount of application of described special fertilizer A is 500ml/m 2.
8. application process according to claim 6, is characterized in that: each amount of application of described special fertilizer B is 500ml/m 2.
9. the application process according to any one of claim 6-8, is characterized in that: the described stem of noble dendrobium is medicinal dendrobium.
10. application process according to claim 9, is characterized in that: described medicinal dendrobium is Herba Dendrobii, dendrobium stem, purple dendrobium or Dendrobium chrysotoxum.
CN201610093557.3A 2016-02-19 2016-02-19 Fertilizer for increasing polysaccharide content in noble dendrobium and applying method thereof Pending CN105503358A (en)

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106565323A (en) * 2016-10-27 2017-04-19 安徽省霍山县祥佑堂中药材开发有限公司 Special liquid fertilizer for dendrobe in field planting period
CN106588385A (en) * 2016-12-28 2017-04-26 刘义存 Raw materials of humic acid water-soluble fertilizer for medicinal dendrobium plants and prepration method thereof
CN106576551A (en) * 2016-12-23 2017-04-26 江苏农林职业技术学院 Fertilizing method for improving polysaccharide content of dendrobium officinale
CN106747813A (en) * 2016-12-28 2017-05-31 刘义存 A kind of medicinal dendrobium biological organic inorganic composite fertiliser and its raw material and preparation method
CN108249981A (en) * 2016-12-28 2018-07-06 广东国方医药科技有限公司 A kind of dendrobium candidum humic acid water-soluble fertilizer and its raw material and preparation method
CN108610170A (en) * 2016-12-28 2018-10-02 广东国方医药科技有限公司 A kind of dendrobium candidum biological organic fertilizer and its raw material and preparation method

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CN104303672A (en) * 2014-11-22 2015-01-28 苏少中 Dendrobium devonianum planting fertilizing method
CN104355739A (en) * 2014-10-31 2015-02-18 恩施茂和食品有限公司 Mung bean foliar fertilizer containing trace element selenium and technique for cultivating selenium-enriched mung beans by utilizing mung bean foliar fertilizer
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CN1765847A (en) * 2005-09-01 2006-05-03 宋家永 Selenium-enriched foliage fertilizer
CN103130559A (en) * 2011-11-25 2013-06-05 施可丰化工股份有限公司 Broad spectrum type leaf fertilizer
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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106565323A (en) * 2016-10-27 2017-04-19 安徽省霍山县祥佑堂中药材开发有限公司 Special liquid fertilizer for dendrobe in field planting period
CN106576551A (en) * 2016-12-23 2017-04-26 江苏农林职业技术学院 Fertilizing method for improving polysaccharide content of dendrobium officinale
CN106588385A (en) * 2016-12-28 2017-04-26 刘义存 Raw materials of humic acid water-soluble fertilizer for medicinal dendrobium plants and prepration method thereof
CN106747813A (en) * 2016-12-28 2017-05-31 刘义存 A kind of medicinal dendrobium biological organic inorganic composite fertiliser and its raw material and preparation method
CN108249981A (en) * 2016-12-28 2018-07-06 广东国方医药科技有限公司 A kind of dendrobium candidum humic acid water-soluble fertilizer and its raw material and preparation method
CN108610170A (en) * 2016-12-28 2018-10-02 广东国方医药科技有限公司 A kind of dendrobium candidum biological organic fertilizer and its raw material and preparation method

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Application publication date: 20160420